Rh4AG085100

serine carboxypeptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000839
Physical Location & Seq
Forward (+)
17779723 .. 17791769
12047 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG085100.1

Sequence Viewer

Length: 1482 bp
ATGACGAAATTGATGGGTATGCGACTACTTCTTGCATTTGTTTTCACTCTCATACTTGCTTCAACTTCTTCGGCCAATGTCACTACCACCACAATTACCACACTACCTGGTTACTCAGGCGACCTCCCTTTCTCCTTCGAAACTGGGTACGTTGGTGTAGGTGACAATGAAGAAGTGCAGCTATTTTACTACTTTGTCGAGTCTCAGAGGACTCCAGCACAGGATCCTCTGCTGTTGTGGATCCCTGCGGGACCTGGTTGCTCTGGTCAGATTGTACTCTTCTTTGAGAGTGGTCCACTACTTTTCGATTATGGTGAATACGATGGGAGCCTACCAACACTTCACGACAACCCTTTCACATGGACGGAGAGCCTCAACCTCATATACGTGGATGCACCTGTTGGTGCTGGATACTCCTACTCAACAACCCAAAGTGGTTATGTAATGGACGACTATAAACATGTGGCACAACTATATGAATTTCTGCAGAAGTGGTTAGATGCACACCCTACGTACCTGAAAAACATACTGTACATTGGTGGTGATTCCTACTCTGGAATGCCTGTTCCCATGCTTGCACAAACAATAGTCAATGGTAATGAAAATGGAAGTATCCCCATGATGAATTTCAGAGGATATGTGATTGGGAACCCAGTAACTGATACAGCAGTTGATGGTAATGCACAAATTCCTATTGCTCACCGGCTGTCTCTTATATCGGATCAACTATATAAGTCACTTAAAACAAGTTGCCACGGCGAATATGTAACTGTAAATTCCAGCAATCATGCATGCATTGCAGATCTTGATGCCTTTGATGCACTAACGAGTGACATAAATACATTACATGTCTTGGAACCATTATGTAGTAATGGTTTACCTACACAAAAGGAAATGGTTTTGACCCGGAGATATCTCAAGGAAACATCTAGCGGTTTACAAAGATCACGACCTAACGGCCCTGCACTTTGGTGTCGGAGTTATCGTAGCATGCTCAACACCGTTTGGGCTAACAACAAAGGTGTTCAAGATGCTCTTGGTGTCCGAAGGGGCACGAAAAGGCTTTGGCAACACTGTAATACGACATTGGACTACACTGAAGATGTCACCAGCGTAATCATTTATCACCAAAGTCTTTCAAAAATGGCTGATCTACGTGTTCTGATATACAGTGGTGATCATGACATGCAAGTTCCTCATATTGGTACACAAAGATGGATAAGCACACTGAATTTGACTGAGGATGAATCCTGGAGGACATGGTCAGTTGATGGCCAAACTGCAGGATACACGAAGAAGTTGATCAATGAATATATCACTTTGACATATGCGACTGTAAAGGGAGGAGGCCATATTGCTGCAGAGACAAAGTATGAGAACGAAAATAATAACCACAATTTACCTTCTCTTATTTTCATCTTCTCCATCTCAACAACAATCAGCAACCAGCCTAAAGCTGTTGACCACTTTGTCAAAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

493

Amino Acids

54.9

Weight (kDa)

5.42

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 36 - 458 1.7e-106 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1469
AciI CCGC 2 cut(s) 248, 933
AclWI GGATC 5 cut(s) 218, 231, 235, 248, 729
AcoI YGGCCR 2 cut(s) 72, 1273
AcsI RAATTY 5 cut(s) 479, 625, 687, 775, 1231
AcuI CTGAAG 1 cut(s) 1119
AfaI GTAC 5 cut(s) 149, 276, 515, 533, 1207
AfiI CCNNNNNNNGG 1 cut(s) 1202
AflIII ACRYGT 3 cut(s) 460, 847, 1156
AgsI TTSAA 3 cut(s) 63, 1028, 1140
AjnI CCWGG 3 cut(s) 106, 253, 1250
AleI CACNNNNGTG 1 cut(s) 970
AloI GAACNNNNNNTCC 2 cut(s) 549, 581
AluBI AGCT 2 cut(s) 181, 1457
AluI AGCT 2 cut(s) 181, 1457
Alw26I GTCTC 3 cut(s) 207, 714, 1358
AlwI GGATC 5 cut(s) 218, 231, 235, 248, 729
AlwNI CAGNNNCTG 1 cut(s) 659
AoxI GGCC 4 cut(s) 72, 958, 1273, 1348
ApeKI GCWGC 2 cut(s) 178, 1358
ApoI RAATTY 5 cut(s) 479, 625, 687, 775, 1231
AspS9I GGNCC 3 cut(s) 251, 293, 959
AsuC2I CCSGG 1 cut(s) 907
AsuHPI GGTGA 7 cut(s) 173, 326, 554, 692, 1099, 1118, 1187
AsuII TTCGAA 1 cut(s) 138
AvaII GGWCC 2 cut(s) 251, 293
BaeGI GKGCMC 1 cut(s) 1055
BaeI ACNNNNGTAYC 4 cut(s) 139, 172, 654, 687
BalI TGGCCA 1 cut(s) 1275
BamHI GGATCC 2 cut(s) 223, 240
BbvI GCAGC 2 cut(s) 190, 1345
BccI CCATC 6 cut(s) 7, 317, 668, 1209, 1265, 1433
BceAI ACGGC 2 cut(s) 772, 973
BciT130I CCWGG 3 cut(s) 108, 255, 1252
BciVI GTATCC 3 cut(s) 404, 623, 1280
BclI TGATCA 2 cut(s) 1177, 1302
BcnI CCSGG 1 cut(s) 907
BcoDI GTCTC 3 cut(s) 207, 714, 1358
BfaI CTAG 1 cut(s) 930
BfmI CTRYAG 3 cut(s) 485, 1281, 1359
BfuI GTATCC 3 cut(s) 404, 623, 1280
BglII AGATCT 1 cut(s) 802
BisI GCNGC 2 cut(s) 179, 1359
BlsI GCNGC 2 cut(s) 180, 1360
Bme1390I CCNGG 4 cut(s) 108, 255, 907, 1252
Bme18I GGWCC 2 cut(s) 251, 293
BmgT120I GGNCC 3 cut(s) 251, 293, 959
BmiI GGNNCC 6 cut(s) 225, 242, 252, 329, 650, 858
BmrFI CCNGG 4 cut(s) 108, 255, 907, 1252
BmrI ACTGGG 2 cut(s) 153, 647
BmsI GCATC 5 cut(s) 382, 490, 799, 808, 1021
BmuI ACTGGG 2 cut(s) 153, 647
BpmI CTGGAG 2 cut(s) 198, 1273
Bpu14I TTCGAA 1 cut(s) 138
BpuEI CTTGAG 1 cut(s) 902
BpuMI CCSGG 1 cut(s) 907
BsaAI YACGTR 3 cut(s) 388, 513, 1157
BsaJI CCNNGG 1 cut(s) 754
BsaXI ACNNNNNCTCC 2 cut(s) 970, 1000
Bsc4I CCNNNNNNNGG 1 cut(s) 1202
Bse118I RCCGGY 1 cut(s) 702
Bse1I ACTGG 2 cut(s) 148, 653
Bse3DI GCAATG 1 cut(s) 795
BseBI CCWGG 3 cut(s) 108, 255, 1252
BseDI CCNNGG 1 cut(s) 754
BseGI GGATG 2 cut(s) 397, 1249
BseLI CCNNNNNNNGG 1 cut(s) 1202
BseMI GCAATG 1 cut(s) 795
BseMII CTCAG 3 cut(s) 129, 218, 1230
BseNI ACTGG 2 cut(s) 148, 653
BseRI GAGGAG 1 cut(s) 1359
BseSI GKGCMC 1 cut(s) 1055
BseXI GCAGC 2 cut(s) 190, 1345
BsgI GTGCAG 2 cut(s) 197, 948
BshFI GGCC 4 cut(s) 74, 960, 1275, 1350
BsiSI CCGG 2 cut(s) 703, 907
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 1 cut(s) 1202
BsmAI GTCTC 3 cut(s) 207, 714, 1358
BsmFI GGGAC 1 cut(s) 264
BsmI GAATGC 1 cut(s) 564
BsnI GGCC 4 cut(s) 74, 960, 1275, 1350
Bsp119I TTCGAA 1 cut(s) 138
Bsp1286I GDGCHC 1 cut(s) 1055
Bsp1407I TGTACA 1 cut(s) 531
Bsp143I GATC 8 cut(s) 223, 240, 721, 802, 944, 1150, 1177, 1302
BspACI CCGC 2 cut(s) 248, 933
BspANI GGCC 4 cut(s) 74, 960, 1275, 1350
BspCNI CTCAG 3 cut(s) 128, 217, 1231
BspHI TCATGA 1 cut(s) 1180
BspLI GGNNCC 6 cut(s) 225, 242, 252, 329, 650, 858
BspMAI CTGCAG 3 cut(s) 489, 1285, 1363
BspPI GGATC 5 cut(s) 218, 231, 235, 248, 729
BspT104I TTCGAA 1 cut(s) 138
BsrDI GCAATG 1 cut(s) 795
BsrFI RCCGGY 1 cut(s) 702
BsrGI TGTACA 1 cut(s) 531
BsrI ACTGG 2 cut(s) 148, 653
BssAI RCCGGY 1 cut(s) 702
BssECI CCNNGG 1 cut(s) 754
BssMI GATC 8 cut(s) 223, 240, 721, 802, 944, 1150, 1177, 1302
Bst2UI CCWGG 3 cut(s) 108, 255, 1252
Bst4CI ACNGT 6 cut(s) 531, 772, 1003, 1076, 1172, 1336
Bst6I CTCTTC 1 cut(s) 284
BstAPI GCANNNNNTGC 1 cut(s) 797
BstAUI TGTACA 1 cut(s) 531
BstBAI YACGTR 3 cut(s) 388, 513, 1157
BstBI TTCGAA 1 cut(s) 138
BstC8I GCNNGC 3 cut(s) 576, 793, 992
BstDEI CTNAG 3 cut(s) 115, 204, 1239
BstDSI CCRYGG 1 cut(s) 754
BstF5I GGATG 2 cut(s) 397, 1249
BstKTI GATC 8 cut(s) 226, 243, 724, 805, 947, 1153, 1180, 1305
BstMAI GTCTC 3 cut(s) 207, 714, 1358
BstMBI GATC 8 cut(s) 223, 240, 721, 802, 944, 1150, 1177, 1302
BstMWI GCNNNNNNNGC 2 cut(s) 797, 818
BstNI CCWGG 3 cut(s) 108, 255, 1252
BstNSI RCATGY 5 cut(s) 464, 795, 851, 994, 1189
BstSCI CCNGG 4 cut(s) 106, 253, 905, 1250
BstSFI CTRYAG 3 cut(s) 485, 1281, 1359
BstSLI GKGCMC 1 cut(s) 1055
BstSNI TACGTA 1 cut(s) 513
BstV1I GCAGC 2 cut(s) 190, 1345
BstX2I RGATCY 3 cut(s) 223, 240, 802
BstYI RGATCY 3 cut(s) 223, 240, 802
BsuI GTATCC 3 cut(s) 404, 623, 1280
BsuRI GGCC 4 cut(s) 74, 960, 1275, 1350
BtgI CCRYGG 1 cut(s) 754
BtsCI GGATG 2 cut(s) 397, 1249
BtsIMutI CAGTG 4 cut(s) 1072, 1095, 1177, 1226
Cac8I GCNNGC 3 cut(s) 576, 793, 992
CaiI CAGNNNCTG 1 cut(s) 659
CciI TCATGA 1 cut(s) 1180
Cfr10I RCCGGY 1 cut(s) 702
Cfr13I GGNCC 3 cut(s) 251, 293, 959
CsiI ACCWGGT 2 cut(s) 106, 253
Csp6I GTAC 5 cut(s) 148, 275, 514, 532, 1206
CviQI GTAC 5 cut(s) 148, 275, 514, 532, 1206
DdeI CTNAG 3 cut(s) 115, 204, 1239
DpnI GATC 8 cut(s) 225, 242, 723, 804, 946, 1152, 1179, 1304
DpnII GATC 8 cut(s) 223, 240, 721, 802, 944, 1150, 1177, 1302
DrdI GACNNNNNNGTC 1 cut(s) 1469
DseDI GACNNNNNNGTC 1 cut(s) 1469
EaeI YGGCCR 2 cut(s) 72, 1273
Eam1104I CTCTTC 1 cut(s) 284
EarI CTCTTC 1 cut(s) 284
Eco105I TACGTA 1 cut(s) 513
Eco32I GATATC 1 cut(s) 914
Eco47I GGWCC 2 cut(s) 251, 293
Eco57I CTGAAG 1 cut(s) 1119
EcoO109I RGGNCCY 1 cut(s) 251
EcoRII CCWGG 3 cut(s) 106, 253, 1250
EcoRV GATATC 1 cut(s) 914
EcoT22I ATGCAT 2 cut(s) 793, 797
FalI AAGNNNNNCTT 2 cut(s) 1020, 1052
FaqI GGGAC 1 cut(s) 264
FauI CCCGC 1 cut(s) 241
FauNDI CATATG 1 cut(s) 1327
FbaI TGATCA 2 cut(s) 1177, 1302
Fnu4HI GCNGC 2 cut(s) 179, 1359
FokI GGATG 2 cut(s) 404, 1256
Fsp4HI GCNGC 2 cut(s) 179, 1359
FspBI CTAG 1 cut(s) 930
GluI GCNGC 2 cut(s) 179, 1359
GsuI CTGGAG 2 cut(s) 198, 1273
HaeIII GGCC 4 cut(s) 74, 960, 1275, 1350
HapII CCGG 2 cut(s) 703, 907
HincII GTYRAC 1 cut(s) 1462
HindII GTYRAC 1 cut(s) 1462
HinfI GANTC 4 cut(s) 200, 211, 545, 1247
HpaII CCGG 2 cut(s) 703, 907
HphI GGTGA 7 cut(s) 173, 326, 554, 692, 1099, 1118, 1187
Hpy166II GTNNAC 5 cut(s) 296, 878, 938, 1208, 1462
Hpy188I TCNGA 7 cut(s) 207, 270, 632, 721, 978, 1046, 1164
Hpy188III TCNNGA 6 cut(s) 344, 555, 806, 948, 1028, 1181
Hpy8I GTNNAC 5 cut(s) 296, 878, 938, 1208, 1462
HpyAV CCTTC 3 cut(s) 145, 1041, 1413
HpyCH4III ACNGT 6 cut(s) 531, 772, 1003, 1076, 1172, 1336
HpyCH4IV ACGT 4 cut(s) 150, 387, 512, 1156
HpyF10VI GCNNNNNNNGC 2 cut(s) 797, 818
HpyF3I CTNAG 3 cut(s) 115, 204, 1239
HpySE526I ACGT 4 cut(s) 150, 387, 512, 1156
Ksp22I TGATCA 2 cut(s) 1177, 1302
Kzo9I GATC 8 cut(s) 223, 240, 721, 802, 944, 1150, 1177, 1302
LmnI GCTCC 1 cut(s) 327
Lsp1109I GCAGC 2 cut(s) 190, 1345
LweI GCATC 5 cut(s) 382, 490, 799, 808, 1021
MabI ACCWGGT 2 cut(s) 106, 253
MaeI CTAG 1 cut(s) 930
MaeII ACGT 4 cut(s) 150, 387, 512, 1156
MaeIII GTNAC 8 cut(s) 79, 110, 161, 655, 735, 766, 830, 1105
MalI GATC 8 cut(s) 225, 242, 723, 804, 946, 1152, 1179, 1304
MboI GATC 8 cut(s) 223, 240, 721, 802, 944, 1150, 1177, 1302
MboII GAAGA 6 cut(s) 60, 182, 271, 1112, 1306, 1411
MflI RGATCY 3 cut(s) 223, 240, 802
MhlI GDGCHC 1 cut(s) 1055
MlsI TGGCCA 1 cut(s) 1275
MluCI AATT 8 cut(s) 8, 93, 479, 625, 687, 775, 1231, 1396
MluNI TGGCCA 1 cut(s) 1275
MlyI GAGTC 2 cut(s) 205, 209
MmeI TCCRAC 1 cut(s) 956
Mox20I TGGCCA 1 cut(s) 1275
Mph1103I ATGCAT 2 cut(s) 793, 797
MscI TGGCCA 1 cut(s) 1275
MseI TTAA 1 cut(s) 741
MslI CAYNNNNRTG 3 cut(s) 386, 970, 1213
Msp20I TGGCCA 1 cut(s) 1275
MspI CCGG 2 cut(s) 703, 907
MspR9I CCNGG 4 cut(s) 108, 255, 907, 1252
Mva1269I GAATGC 1 cut(s) 564
MvaI CCWGG 3 cut(s) 108, 255, 1252
MwoI GCNNNNNNNGC 2 cut(s) 797, 818
NciI CCSGG 1 cut(s) 907
NdeI CATATG 1 cut(s) 1327
NdeII GATC 8 cut(s) 223, 240, 721, 802, 944, 1150, 1177, 1302
NlaIV GGNNCC 6 cut(s) 225, 242, 252, 329, 650, 858
NmuCI GTSAC 5 cut(s) 79, 161, 735, 830, 1105
NsiI ATGCAT 2 cut(s) 793, 797
NspI RCATGY 5 cut(s) 464, 795, 851, 994, 1189
NspV TTCGAA 1 cut(s) 138
OliI CACNNNNGTG 1 cut(s) 970
PaeI GCATGC 2 cut(s) 795, 994
PagI TCATGA 1 cut(s) 1180
PciI ACATGT 2 cut(s) 460, 847
PcsI WCGNNNNNNNCGW 1 cut(s) 982
PctI GAATGC 1 cut(s) 564
PfeI GAWTC 2 cut(s) 545, 1247
PflFI GACNNNGTC 1 cut(s) 1261
PfoI TCCNGGA 1 cut(s) 1250
PkrI GCNGC 2 cut(s) 180, 1360
PleI GAGTC 2 cut(s) 205, 208
PpsI GAGTC 2 cut(s) 205, 208
Ppu21I YACGTR 3 cut(s) 388, 513, 1157
PpuMI RGGWCCY 1 cut(s) 251
PscI ACATGT 2 cut(s) 460, 847
Psp5II RGGWCCY 1 cut(s) 251
Psp6I CCWGG 3 cut(s) 106, 253, 1250
PspGI CCWGG 3 cut(s) 106, 253, 1250
PspN4I GGNNCC 6 cut(s) 225, 242, 252, 329, 650, 858
PspPI GGNCC 3 cut(s) 251, 293, 959
PspPPI RGGWCCY 1 cut(s) 251
PstI CTGCAG 3 cut(s) 489, 1285, 1363
PstNI CAGNNNCTG 1 cut(s) 659
PsuI RGATCY 3 cut(s) 223, 240, 802
PsyI GACNNNGTC 1 cut(s) 1261
RsaI GTAC 5 cut(s) 149, 276, 515, 533, 1207
RsaNI GTAC 5 cut(s) 148, 275, 514, 532, 1206
RseI CAYNNNNRTG 3 cut(s) 386, 970, 1213
SaqAI TTAA 1 cut(s) 741
SatI GCNGC 2 cut(s) 179, 1359
Sau3AI GATC 8 cut(s) 223, 240, 721, 802, 944, 1150, 1177, 1302
Sau96I GGNCC 3 cut(s) 251, 293, 959
SchI GAGTC 2 cut(s) 205, 209
ScrFI CCNGG 4 cut(s) 108, 255, 907, 1252
SduI GDGCHC 1 cut(s) 1055
SexAI ACCWGGT 2 cut(s) 106, 253
SfaNI GCATC 5 cut(s) 382, 490, 799, 808, 1021
SfcI CTRYAG 3 cut(s) 485, 1281, 1359
SfuI TTCGAA 1 cut(s) 138
SinI GGWCC 2 cut(s) 251, 293
SmiMI CAYNNNNRTG 3 cut(s) 386, 970, 1213
SmlI CTYRAG 1 cut(s) 917
SmoI CTYRAG 1 cut(s) 917
SnaBI TACGTA 1 cut(s) 513
SphI GCATGC 2 cut(s) 795, 994
Sse9I AATT 8 cut(s) 8, 93, 479, 625, 687, 775, 1231, 1396
SsiI CCGC 2 cut(s) 248, 933
SspMI CTAG 1 cut(s) 930
StyD4I CCNGG 4 cut(s) 106, 253, 905, 1250
TaaI ACNGT 6 cut(s) 531, 772, 1003, 1076, 1172, 1336
TaiI ACGT 4 cut(s) 153, 390, 515, 1159
TaqI TCGA 3 cut(s) 138, 198, 306
TasI AATT 8 cut(s) 8, 93, 479, 625, 687, 775, 1231, 1396
TatI WGTACW 2 cut(s) 274, 531
TfiI GAWTC 2 cut(s) 545, 1247
Tru1I TTAA 1 cut(s) 741
Tru9I TTAA 1 cut(s) 741
TscAI CASTG 4 cut(s) 1079, 1102, 1177, 1233
TseFI GTSAC 5 cut(s) 79, 161, 735, 830, 1105
TseI GCWGC 2 cut(s) 178, 1358
Tsp45I GTSAC 5 cut(s) 79, 161, 735, 830, 1105
TspDTI ATGAA 7 cut(s) 183, 492, 615, 638, 1260, 1323, 1405
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 4 cut(s) 1079, 1102, 1177, 1233
Tth111I GACNNNGTC 1 cut(s) 1261
VpaK11BI GGWCC 2 cut(s) 251, 293
XapI RAATTY 5 cut(s) 479, 625, 687, 775, 1231
XceI RCATGY 5 cut(s) 464, 795, 851, 994, 1189
XspI CTAG 1 cut(s) 930
Zsp2I ATGCAT 2 cut(s) 793, 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.