Rorug04G0030500

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000839
Physical Location & Seq
Forward (+)
4399301 .. 4403585
4285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0030500.1

Sequence Viewer

Length: 1632 bp
ATGCTAGGCCTAGCACACAATGTGGCTACCAATAAGGTAGCCTCAGTAGTCCACCCTACTAATCAGAAAACCATTGCTGGGGAGCTTAGCCTGTCTGACAAGAAGATCTTGGAGGAGATTTACACAACTCATGTCCACGCTGATGAATCCTTTGATGACGATTCTCTCTTCGTCATTGTTGAGAACATCCTCAAGCGTGCAACCCAAAATGTTGATAAAATAGTGCAGGGTACTCAAGTGCATGTGGACAGCATAGAGGAGAAAACCCCCAAAGCCAGTTTTAGAGTACCATTGTGCACGCTAAAGGCAATCTCCTGCGAGATGCAATGCAAGCCTCCTGGTGACGAAATCGCCCACAACACAACAGTGGCAATACTCAACAAGCTCTCAAAGTATTCATGGGAAGCAAAGGCATCACTGACCCTAGCAGCTTTTGCTATGGAATATGAGGAGGCAGAGACTTACAGAAGGATCACGAAATTGTTCCGAACCCCTACTGAAATTATGGAGGTTTTCAAGGGATTAATATTTACAAAGGATAATGTCCAGCCACTCATCGATGGTTCAACTAAGCAAACGGTTAAGATCGACATCCTAAGGAGAAAGAATGTGTTATTGTTCATTTCCAGCCTAGACGTCTCCGACGATGACATTTCGATTCTGAAACCGATCCACGAGTTCACAAAGAGAGACGATAAGTATAAGATTGTCTGGATTCCAATTGTCAAGCAATGGACTGATGACCTGAGAAAAAAGTTTGATATCCTGAAGAACAAGATGCCGTGGTTTACGGTGCAGTACTCTGGACCTATAGCAGGCATCAAGTTCATCAAGGAGGAGTGGAATTTCAAGGGTAAGCCTACAGTGGTAGTGATGAACCCACAAGGGAAGGTCGAACACTCTAACGCTCTCCACATGATTCGGGTATGGGGAGCCAAGGCCTACCCTTTCACAAAGACAACAGAAGAAGAACTGTCACATTCTCATGCAGGAAAATGGGTTGGCTCCGTTGTTGAAGGAACTCACCCATCTGTGCACACTTGGATCAAGGAGGATAAGTACATCTTCTTCTATGGCGGTAAAGACAACGAATGGATTCAGCAATTCACAAAGAAAGCGACTGCTCTTGCAAATGATCCAATCTTCAAGGAAGCAAAGATTCACCTAGAGTTGGTCTATGTCGGAAAAGTCAGCAAAGGAGAAGATGACCATGGCGTTCTAGGCCGCTTCTGGACCAGTGTAGAGAGCCTGTTCTTCACCAAAGGCCACAAGCAGGTGGAGTCAGTGAATCAAGAAATCCAAAAGCTTCTCTCTTACAAAAACGAAAGTGGATGGGCGGTGCTTAGCAAAGGGTCGACTGTGTTGGTCACAGGTCATGGTGTCTCGGTGTTGAAGGTGGTTGAGGACTTTGAGAAATGGAAGGACCATGTCAAGGAGAAAGGGTTTGAGTTTTGTTTCAAGGCCTATCATGAGAAGATTAGTCAGGCTAGTCGACCTTGCTGCCGTCTGGACATTCCGGGGTCTAACGGAAAGGTTCCAGAGTCGATGAGGTGCCCTGATTGCCACCGTAATATGGAGACTTTTATCAGTTACAAGTGCTGCCACATTGATGGTCCTACTGCACATCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

543

Amino Acids

61.77

Weight (kDa)

8.31

Isoelectric Point (pI)

25.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_N PF14576 32 - 151 2.9e-36 Sieve element occlusion N-terminus
SEO_C PF14577 337 - 402 2.8e-16 Sieve element occlusion C-terminus
SEO_C PF14577 410 - 535 2.1e-34 Sieve element occlusion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1264
AatII GACGTC 1 cut(s) 639
Acc36I ACCTGC 1 cut(s) 1264
AccB1I GGYRCC 1 cut(s) 1551
AccI GTMKAC 2 cut(s) 1355, 1492
AciI CCGC 3 cut(s) 1077, 1225, 1337
AclWI GGATC 4 cut(s) 479, 664, 1052, 1130
AcsI RAATTY 1 cut(s) 844
AcuI CTGAAG 1 cut(s) 788
AcyI GRCGYC 1 cut(s) 636
AdeI CACNNNGTG 1 cut(s) 22
AfaI GTAC 4 cut(s) 232, 288, 800, 1061
AfiI CCNNNNNNNGG 6 cut(s) 78, 815, 1171, 1273, 1432, 1573
AgsI TTSAA 7 cut(s) 517, 567, 850, 1016, 1147, 1393, 1459
AjnI CCWGG 1 cut(s) 337
AleI CACNNNNGTG 1 cut(s) 365
AluBI AGCT 4 cut(s) 85, 385, 431, 1306
AluI AGCT 4 cut(s) 85, 385, 431, 1306
Alw21I GWGCWC 2 cut(s) 299, 1038
Alw26I GTCTC 5 cut(s) 452, 643, 684, 1387, 1571
Alw44I GTGCAC 2 cut(s) 295, 1034
AlwI GGATC 4 cut(s) 479, 664, 1052, 1130
AoxI GGCC 5 cut(s) 7, 939, 1222, 1264, 1461
ApaLI GTGCAC 2 cut(s) 295, 1034
ApeKI GCWGC 3 cut(s) 428, 1500, 1599
ApoI RAATTY 1 cut(s) 844
AseI ATTAAT 1 cut(s) 524
Asp700I GAANNNNTTC 2 cut(s) 482, 1095
AspS9I GGNCC 4 cut(s) 806, 1233, 1423, 1613
AsuC2I CCSGG 1 cut(s) 1518
AsuHPI GGTGA 4 cut(s) 353, 1016, 1154, 1249
AvaII GGWCC 4 cut(s) 806, 1233, 1423, 1613
AxyI CCTNAGG 1 cut(s) 596
BaeGI GKGCMC 3 cut(s) 299, 1038, 1556
BaeI ACNNNNGTAYC 2 cut(s) 222, 255
BanI GGYRCC 1 cut(s) 1551
BarI GAAGNNNNNNTAC 2 cut(s) 1052, 1084
BauI CACGAG 1 cut(s) 674
Bbv12I GWGCWC 2 cut(s) 299, 1038
BbvI GCAGC 3 cut(s) 440, 1487, 1586
BccI CCATC 4 cut(s) 554, 1036, 1326, 1604
BceAI ACGGC 2 cut(s) 766, 1488
BcgI CGANNNNNNTGC 2 cut(s) 1482, 1516
BciT130I CCWGG 1 cut(s) 339
BcnI CCSGG 1 cut(s) 1518
BcoDI GTCTC 5 cut(s) 452, 643, 684, 1387, 1571
BfaI CTAG 7 cut(s) 5, 11, 425, 632, 1166, 1220, 1488
BfmI CTRYAG 2 cut(s) 810, 861
BfuAI ACCTGC 1 cut(s) 1264
BglII AGATCT 1 cut(s) 105
BisI GCNGC 4 cut(s) 429, 1225, 1501, 1600
BlpI GCTNAGC 2 cut(s) 86, 1343
BlsI GCNGC 4 cut(s) 430, 1226, 1502, 1601
BmcAI AGTACT 1 cut(s) 800
Bme1390I CCNGG 2 cut(s) 339, 1518
Bme18I GGWCC 4 cut(s) 806, 1233, 1423, 1613
BmgT120I GGNCC 4 cut(s) 806, 1233, 1423, 1613
BmiI GGNNCC 4 cut(s) 934, 1006, 1536, 1553
BmrFI CCNGG 2 cut(s) 339, 1518
BmsI GCATC 4 cut(s) 312, 422, 768, 828
Bpu1102I GCTNAGC 2 cut(s) 86, 1343
BpuEI CTTGAG 2 cut(s) 176, 219
BpuMI CCSGG 1 cut(s) 1518
Bsa29I ATCGAT 1 cut(s) 558
BsaBI GATNNNNATC 1 cut(s) 590
BsaHI GRCGYC 1 cut(s) 636
BsaJI CCNNGG 4 cut(s) 782, 936, 1210, 1517
BsaXI ACNNNNNCTCC 4 cut(s) 1043, 1073, 1427, 1457
Bsc4I CCNNNNNNNGG 6 cut(s) 78, 815, 1171, 1273, 1432, 1573
Bse1I ACTGG 2 cut(s) 276, 1236
Bse21I CCTNAGG 1 cut(s) 596
Bse3DI GCAATG 3 cut(s) 72, 332, 737
Bse8I GATNNNNATC 1 cut(s) 590
BseBI CCWGG 1 cut(s) 339
BseCI ATCGAT 1 cut(s) 558
BseDI CCNNGG 4 cut(s) 782, 936, 1210, 1517
BseGI GGATG 3 cut(s) 186, 591, 1337
BseJI GATNNNNATC 1 cut(s) 590
BseLI CCNNNNNNNGG 6 cut(s) 78, 815, 1171, 1273, 1432, 1573
BseMI GCAATG 3 cut(s) 72, 332, 737
BseMII CTCAG 2 cut(s) 57, 737
BseNI ACTGG 2 cut(s) 276, 1236
BseRI GAGGAG 4 cut(s) 128, 272, 464, 851
BseSI GKGCMC 3 cut(s) 299, 1038, 1556
BseXI GCAGC 3 cut(s) 440, 1487, 1586
BseYI CCCAGC 1 cut(s) 77
BsgI GTGCAG 3 cut(s) 245, 815, 1605
BshFI GGCC 5 cut(s) 9, 941, 1224, 1266, 1463
BshNI GGYRCC 1 cut(s) 1551
BshVI ATCGAT 1 cut(s) 558
BsiHKAI GWGCWC 2 cut(s) 299, 1038
BsiSI CCGG 1 cut(s) 1517
BslI CCNNNNNNNGG 6 cut(s) 78, 815, 1171, 1273, 1432, 1573
BsmAI GTCTC 5 cut(s) 452, 643, 684, 1387, 1571
BsmBI CGTCTC 2 cut(s) 643, 684
BsnI GGCC 5 cut(s) 9, 941, 1224, 1266, 1463
Bsp1286I GDGCHC 3 cut(s) 299, 1038, 1556
Bsp143I GATC 6 cut(s) 105, 471, 585, 669, 1044, 1135
Bsp1720I GCTNAGC 2 cut(s) 86, 1343
Bsp19I CCATGG 1 cut(s) 1210
BspACI CCGC 3 cut(s) 1077, 1225, 1337
BspANI GGCC 5 cut(s) 9, 941, 1224, 1266, 1463
BspCNI CTCAG 2 cut(s) 56, 738
BspDI ATCGAT 1 cut(s) 558
BspHI TCATGA 1 cut(s) 1468
BspLI GGNNCC 4 cut(s) 934, 1006, 1536, 1553
BspMI ACCTGC 1 cut(s) 1264
BspPI GGATC 4 cut(s) 479, 664, 1052, 1130
BspT107I GGYRCC 1 cut(s) 1551
BsrDI GCAATG 3 cut(s) 72, 332, 737
BsrI ACTGG 2 cut(s) 276, 1236
BssECI CCNNGG 4 cut(s) 782, 936, 1210, 1517
BssMI GATC 6 cut(s) 105, 471, 585, 669, 1044, 1135
BssNI GRCGYC 1 cut(s) 636
BssSI CACGAG 1 cut(s) 674
BssT1I CCWWGG 2 cut(s) 936, 1210
Bst2BI CACGAG 1 cut(s) 674
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 7 cut(s) 367, 580, 793, 865, 975, 1360, 1568
Bst6I CTCTTC 1 cut(s) 173
BstACI GRCGYC 1 cut(s) 636
BstAPI GCANNNNNTGC 1 cut(s) 434
BstC8I GCNNGC 4 cut(s) 198, 299, 332, 817
BstDEI CTNAG 6 cut(s) 43, 86, 570, 596, 746, 1343
BstDSI CCRYGG 2 cut(s) 782, 1210
BstENI CCTNNNNNAGG 1 cut(s) 813
BstF5I GGATG 3 cut(s) 186, 591, 1337
BstKTI GATC 6 cut(s) 108, 474, 588, 672, 1047, 1138
BstMAI GTCTC 5 cut(s) 452, 643, 684, 1387, 1571
BstMBI GATC 6 cut(s) 105, 471, 585, 669, 1044, 1135
BstMWI GCNNNNNNNGC 4 cut(s) 331, 434, 1221, 1560
BstNI CCWGG 1 cut(s) 339
BstNSI RCATGY 1 cut(s) 245
BstSCI CCNGG 2 cut(s) 337, 1516
BstSFI CTRYAG 2 cut(s) 810, 861
BstSLI GKGCMC 3 cut(s) 299, 1038, 1556
BstV1I GCAGC 3 cut(s) 440, 1487, 1586
BstX2I RGATCY 1 cut(s) 105
BstXI CCANNNNNNTGG 1 cut(s) 1610
BstYI RGATCY 1 cut(s) 105
Bsu15I ATCGAT 1 cut(s) 558
Bsu36I CCTNAGG 1 cut(s) 596
BsuRI GGCC 5 cut(s) 9, 941, 1224, 1266, 1463
BsuTUI ATCGAT 1 cut(s) 558
BtgI CCRYGG 2 cut(s) 782, 1210
BtsCI GGATG 3 cut(s) 186, 591, 1337
BtsIMutI CAGTG 5 cut(s) 372, 416, 870, 1243, 1290
BveI ACCTGC 1 cut(s) 1264
Cac8I GCNNGC 4 cut(s) 198, 299, 332, 817
CciI TCATGA 1 cut(s) 1468
Cfr13I GGNCC 4 cut(s) 806, 1233, 1423, 1613
ClaI ATCGAT 1 cut(s) 558
Csp6I GTAC 4 cut(s) 231, 287, 799, 1060
CviAII CATG 9 cut(s) 131, 242, 399, 916, 986, 1211, 1376, 1427, 1469
CviQI GTAC 4 cut(s) 231, 287, 799, 1060
DdeI CTNAG 6 cut(s) 43, 86, 570, 596, 746, 1343
DpnI GATC 6 cut(s) 107, 473, 587, 671, 1046, 1137
DpnII GATC 6 cut(s) 105, 471, 585, 669, 1044, 1135
DraIII CACNNNGTG 1 cut(s) 22
Eam1104I CTCTTC 1 cut(s) 173
EarI CTCTTC 1 cut(s) 173
Eco130I CCWWGG 2 cut(s) 936, 1210
Eco147I AGGCCT 3 cut(s) 9, 941, 1463
Eco32I GATATC 1 cut(s) 763
Eco47I GGWCC 4 cut(s) 806, 1233, 1423, 1613
Eco57I CTGAAG 1 cut(s) 788
Eco81I CCTNAGG 1 cut(s) 596
EcoNI CCTNNNNNAGG 1 cut(s) 813
EcoRII CCWGG 1 cut(s) 337
EcoRV GATATC 1 cut(s) 763
EcoT14I CCWWGG 2 cut(s) 936, 1210
ErhI CCWWGG 2 cut(s) 936, 1210
Esp3I CGTCTC 2 cut(s) 643, 684
FaeI CATG 9 cut(s) 134, 245, 402, 919, 989, 1214, 1379, 1430, 1472
FalI AAGNNNNNCTT 4 cut(s) 92, 124, 1049, 1081
FatI CATG 9 cut(s) 130, 241, 398, 915, 985, 1210, 1375, 1426, 1468
FblI GTMKAC 2 cut(s) 1355, 1492
Fnu4HI GCNGC 4 cut(s) 429, 1225, 1501, 1600
FokI GGATG 3 cut(s) 173, 578, 1344
Fsp4HI GCNGC 4 cut(s) 429, 1225, 1501, 1600
FspBI CTAG 7 cut(s) 5, 11, 425, 632, 1166, 1220, 1488
GluI GCNGC 4 cut(s) 429, 1225, 1501, 1600
GsaI CCCAGC 1 cut(s) 81
HaeIII GGCC 5 cut(s) 9, 941, 1224, 1266, 1463
HapII CCGG 1 cut(s) 1517
Hin1I GRCGYC 1 cut(s) 636
Hin1II CATG 9 cut(s) 134, 245, 402, 919, 989, 1214, 1379, 1430, 1472
HincII GTYRAC 2 cut(s) 1356, 1493
HindII GTYRAC 2 cut(s) 1356, 1493
HindIII AAGCTT 1 cut(s) 1304
HpaII CCGG 1 cut(s) 1517
HphI GGTGA 4 cut(s) 353, 1016, 1154, 1249
Hpy166II GTNNAC 9 cut(s) 52, 136, 247, 297, 681, 789, 1036, 1356, 1493
Hpy188I TCNGA 6 cut(s) 66, 97, 488, 643, 663, 1184
Hpy188III TCNNGA 9 cut(s) 475, 712, 766, 804, 1231, 1292, 1469, 1508, 1538
Hpy8I GTNNAC 9 cut(s) 52, 136, 247, 297, 681, 789, 1036, 1356, 1493
Hpy99I CGWCG 1 cut(s) 647
HpyAV CCTTC 5 cut(s) 462, 883, 1010, 1387, 1414
HpyCH4III ACNGT 7 cut(s) 367, 580, 793, 865, 975, 1360, 1568
HpyCH4IV ACGT 1 cut(s) 636
HpyF10VI GCNNNNNNNGC 4 cut(s) 331, 434, 1221, 1560
HpyF3I CTNAG 6 cut(s) 43, 86, 570, 596, 746, 1343
HpySE526I ACGT 1 cut(s) 636
Hsp92I GRCGYC 1 cut(s) 636
Hsp92II CATG 9 cut(s) 134, 245, 402, 919, 989, 1214, 1379, 1430, 1472
Kzo9I GATC 6 cut(s) 105, 471, 585, 669, 1044, 1135
LmnI GCTCC 3 cut(s) 82, 932, 1010
Lsp1109I GCAGC 3 cut(s) 440, 1487, 1586
LweI GCATC 4 cut(s) 312, 422, 768, 828
MaeI CTAG 7 cut(s) 5, 11, 425, 632, 1166, 1220, 1488
MaeII ACGT 1 cut(s) 636
MaeIII GTNAC 4 cut(s) 341, 975, 1366, 1589
MalI GATC 6 cut(s) 107, 473, 587, 671, 1046, 1137
MboI GATC 6 cut(s) 105, 471, 585, 669, 1044, 1135
MfeI CAATTG 1 cut(s) 720
MflI RGATCY 1 cut(s) 105
MhlI GDGCHC 3 cut(s) 299, 1038, 1556
MluCI AATT 5 cut(s) 479, 501, 720, 844, 1103
MlyI GAGTC 2 cut(s) 1289, 1550
MmeI TCCRAC 2 cut(s) 666, 1162
MroXI GAANNNNTTC 2 cut(s) 482, 1095
MseI TTAA 3 cut(s) 524, 582, 1630
MslI CAYNNNNRTG 4 cut(s) 141, 365, 984, 1608
MspI CCGG 1 cut(s) 1517
MspR9I CCNGG 2 cut(s) 339, 1518
MunI CAATTG 1 cut(s) 720
MvaI CCWGG 1 cut(s) 339
MwoI GCNNNNNNNGC 4 cut(s) 331, 434, 1221, 1560
NciI CCSGG 1 cut(s) 1518
NcoI CCATGG 1 cut(s) 1210
NdeII GATC 6 cut(s) 105, 471, 585, 669, 1044, 1135
NlaIII CATG 9 cut(s) 134, 245, 402, 919, 989, 1214, 1379, 1430, 1472
NlaIV GGNNCC 4 cut(s) 934, 1006, 1536, 1553
NmuCI GTSAC 3 cut(s) 341, 975, 1366
NspI RCATGY 1 cut(s) 245
OliI CACNNNNGTG 1 cut(s) 365
PagI TCATGA 1 cut(s) 1468
PaqCI CACCTGC 1 cut(s) 1264
PceI AGGCCT 3 cut(s) 9, 941, 1463
PcsI WCGNNNNNNNCGW 1 cut(s) 642
PdmI GAANNNNTTC 2 cut(s) 482, 1095
PfeI GAWTC 8 cut(s) 146, 161, 658, 715, 919, 1096, 1159, 1288
PflFI GACNNNGTC 1 cut(s) 1427
PkrI GCNGC 4 cut(s) 430, 1226, 1502, 1601
PleI GAGTC 2 cut(s) 1288, 1549
PpsI GAGTC 2 cut(s) 1288, 1549
PshBI ATTAAT 1 cut(s) 524
Psp6I CCWGG 1 cut(s) 337
PspFI CCCAGC 1 cut(s) 77
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 4 cut(s) 934, 1006, 1536, 1553
PspPI GGNCC 4 cut(s) 806, 1233, 1423, 1613
PsuI RGATCY 1 cut(s) 105
PsyI GACNNNGTC 1 cut(s) 1427
RsaI GTAC 4 cut(s) 232, 288, 800, 1061
RsaNI GTAC 4 cut(s) 231, 287, 799, 1060
RseI CAYNNNNRTG 4 cut(s) 141, 365, 984, 1608
SalI GTCGAC 2 cut(s) 1354, 1491
SaqAI TTAA 3 cut(s) 524, 582, 1630
SatI GCNGC 4 cut(s) 429, 1225, 1501, 1600
Sau3AI GATC 6 cut(s) 105, 471, 585, 669, 1044, 1135
Sau96I GGNCC 4 cut(s) 806, 1233, 1423, 1613
ScaI AGTACT 1 cut(s) 800
SchI GAGTC 2 cut(s) 1289, 1550
ScrFI CCNGG 2 cut(s) 339, 1518
SduI GDGCHC 3 cut(s) 299, 1038, 1556
SfaNI GCATC 4 cut(s) 312, 422, 768, 828
SfcI CTRYAG 2 cut(s) 810, 861
SinI GGWCC 4 cut(s) 806, 1233, 1423, 1613
SmiMI CAYNNNNRTG 4 cut(s) 141, 365, 984, 1608
SmlI CTYRAG 2 cut(s) 191, 234
SmoI CTYRAG 2 cut(s) 191, 234
Sse9I AATT 5 cut(s) 479, 501, 720, 844, 1103
SseBI AGGCCT 3 cut(s) 9, 941, 1463
SsiI CCGC 3 cut(s) 1077, 1225, 1337
SspI AATATT 1 cut(s) 528
SspMI CTAG 7 cut(s) 5, 11, 425, 632, 1166, 1220, 1488
StuI AGGCCT 3 cut(s) 9, 941, 1463
StyD4I CCNGG 2 cut(s) 337, 1516
StyI CCWWGG 2 cut(s) 936, 1210
TaaI ACNGT 7 cut(s) 367, 580, 793, 865, 975, 1360, 1568
TaiI ACGT 1 cut(s) 639
TaqI TCGA 7 cut(s) 558, 588, 656, 894, 1355, 1492, 1544
TasI AATT 5 cut(s) 479, 501, 720, 844, 1103
TatI WGTACW 2 cut(s) 798, 1059
TauI GCSGC 1 cut(s) 1227
TfiI GAWTC 8 cut(s) 146, 161, 658, 715, 919, 1096, 1159, 1288
Tru1I TTAA 3 cut(s) 524, 582, 1630
Tru9I TTAA 3 cut(s) 524, 582, 1630
TscAI CASTG 5 cut(s) 372, 423, 870, 1243, 1290
TseFI GTSAC 3 cut(s) 341, 975, 1366
TseI GCWGC 3 cut(s) 428, 1500, 1599
Tsp45I GTSAC 3 cut(s) 341, 975, 1366
TspDTI ATGAA 5 cut(s) 159, 387, 610, 817, 890
TspGWI ACGGA 2 cut(s) 997, 1542
TspRI CASTG 5 cut(s) 372, 423, 870, 1243, 1290
Tth111I GACNNNGTC 1 cut(s) 1427
VneI GTGCAC 2 cut(s) 295, 1034
VpaK11BI GGWCC 4 cut(s) 806, 1233, 1423, 1613
VspI ATTAAT 1 cut(s) 524
XagI CCTNNNNNAGG 1 cut(s) 813
XapI RAATTY 1 cut(s) 844
XceI RCATGY 1 cut(s) 245
XmiI GTMKAC 2 cut(s) 1355, 1492
XmnI GAANNNNTTC 2 cut(s) 482, 1095
XspI CTAG 7 cut(s) 5, 11, 425, 632, 1166, 1220, 1488
ZraI GACGTC 1 cut(s) 637
ZrmI AGTACT 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.