FvH4_4g08280

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000845
Physical Location & Seq
Forward (+)
8162704 .. 8163831
1128 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g08280.t1

Sequence Viewer

Length: 933 bp
ATGGTAGGATTGGGCACACCTAACAAACAACTTTCGCTCGAATTTGACACCGGAAGCTTCCTCACTTGGACTCGATGCCAACCTTGTGTTTCCCCCTGTTGCTACCCACAAAATGATCCCATTTTCGACCCTGCAGCCTCCACCTCATATGCCAAAATTTCATGTAACAGCGCAGAATGCGCCCAACTCAGGAGAAGCCAATGCTCATCAGCCGCAAACACATGCGTATATGAGGTGATCTATGGAGACGAGTCCCACACCAATGGGGTTTTCTCCAAAGACAAGCTGACCTTAACTCCCGCGGACGTATTTGATGGGTTCCTCTTTGGCTGCGGCCAAGATAACGAAGGCTTCCATGATGGCTCGGCCGCAAGCTCCACCGGTTACCTCAGCTTCGGCAGTCCCACCGGAGCTTCCAATGCTGCCATAAAATACACACGGCTCACTACTTTATCCTCTGGCTCAACGTATTACGGCCTTAACCTGGTTGGGATCAATGTGAACGGGCAAAAGCTTTCAATTCCCGCTTCGGTGTTTTCGTCTCCGGGGACGATCATTGACTCCGGGACTGTCATATCACGGCTGCCGCCCACCGCCTTCAGCGCTTTGCGCGACGCATTTCAGGCAGCGATGACAAGCTATCCTTCAGTTCCGGGGGATGGATTCCTAGATACATGCTACGACCTCAACTGCAATCCAACGGTGACGTATCCGAAAATAGAGTTTGTGTTCGGCGACGGGGTTACGTTGACTTTGGACGCAACTGGAATCGTCTACCTTTTGGACGGGACAATGAAGCAGGTTTGCTTGGCTTTTGTTGGGAATGATGATGCAGACGAGCTTACGATATTCGGAAATACTCAGCAGAAGAATATGGAGGTATTGTATGACGTCGCCGGAGAGAGAGTCGGATTTGCTCCCGGTGGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

311

Amino Acids

32.8

Weight (kDa)

4.36

Isoelectric Point (pI)

27.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 2 - 134 7.1e-33 Xylanase inhibitor N-terminal
Asp PF00026 2 - 99 8.8e-06 Eukaryotic aspartyl protease
Asp PF00026 124 - 307 1.3e-15 Eukaryotic aspartyl protease
TAXi_C PF14541 158 - 306 1.7e-28 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 894
Acc36I ACCTGC 1 cut(s) 790
AccI GTMKAC 1 cut(s) 774
AccII CGCG 2 cut(s) 302, 612
AciI CCGC 8 cut(s) 213, 300, 302, 333, 369, 525, 587, 594
AclWI GGATC 2 cut(s) 110, 500
AcoI YGGCCR 2 cut(s) 334, 366
AcsI RAATTY 2 cut(s) 41, 156
AcuI CTGAAG 2 cut(s) 583, 630
AcyI GRCGYC 1 cut(s) 891
AfeI AGCGCT 1 cut(s) 604
AfiI CCNNNNNNNGG 3 cut(s) 189, 484, 659
AgeI ACCGGT 1 cut(s) 380
AgsI TTSAA 1 cut(s) 519
AjnI CCWGG 1 cut(s) 483
AjuI GAANNNNNNNTTGG 2 cut(s) 254, 286
AluBI AGCT 8 cut(s) 57, 286, 375, 393, 413, 514, 639, 841
AluI AGCT 8 cut(s) 57, 286, 375, 393, 413, 514, 639, 841
Alw26I GTCTC 2 cut(s) 240, 546
AlwI GGATC 2 cut(s) 110, 500
Aor51HI AGCGCT 1 cut(s) 604
AoxI GGCC 3 cut(s) 334, 366, 475
ApeKI GCWGC 5 cut(s) 134, 330, 422, 583, 626
ApoI RAATTY 2 cut(s) 41, 156
AsiGI ACCGGT 1 cut(s) 380
AspLEI GCGC 4 cut(s) 173, 182, 605, 612
AsuC2I CCSGG 4 cut(s) 546, 565, 654, 921
AsuHPI GGTGA 2 cut(s) 247, 715
BaeGI GKGCMC 1 cut(s) 17
BbvCI CCTCAGC 1 cut(s) 389
BbvI GCAGC 5 cut(s) 146, 317, 409, 570, 638
BccI CCATC 3 cut(s) 308, 353, 653
BceAI ACGGC 3 cut(s) 455, 490, 596
BciT130I CCWGG 1 cut(s) 485
BciVI GTATCC 1 cut(s) 720
BcnI CCSGG 4 cut(s) 546, 565, 654, 921
BcoDI GTCTC 2 cut(s) 240, 546
BfaI CTAG 1 cut(s) 668
BfmI CTRYAG 1 cut(s) 132
BfoI RGCGCY 1 cut(s) 606
BfuAI ACCTGC 1 cut(s) 790
BfuI GTATCC 1 cut(s) 720
BisI GCNGC 9 cut(s) 135, 213, 331, 334, 369, 423, 584, 587, 627
BlsI GCNGC 9 cut(s) 136, 214, 332, 335, 370, 424, 585, 588, 628
Bme1390I CCNGG 5 cut(s) 485, 546, 565, 654, 921
BmiI GGNNCC 1 cut(s) 320
BmrFI CCNGG 5 cut(s) 485, 546, 565, 654, 921
BmsI GCATC 2 cut(s) 65, 820
Bpu10I CCTNAGC 1 cut(s) 389
BpuMI CCSGG 4 cut(s) 546, 565, 654, 921
BsaHI GRCGYC 1 cut(s) 891
BsaJI CCNNGG 3 cut(s) 300, 545, 653
BsaWI WCCGGW 3 cut(s) 50, 380, 407
BsaXI ACNNNNNCTCC 6 cut(s) 280, 310, 869, 891, 899, 921
Bsc4I CCNNNNNNNGG 3 cut(s) 189, 484, 659
Bse118I RCCGGY 1 cut(s) 380
Bse1I ACTGG 1 cut(s) 769
BseBI CCWGG 1 cut(s) 485
BseDI CCNNGG 3 cut(s) 300, 545, 653
BseGI GGATG 1 cut(s) 664
BseLI CCNNNNNNNGG 3 cut(s) 189, 484, 659
BseMII CTCAG 3 cut(s) 202, 403, 875
BseNI ACTGG 1 cut(s) 769
BseSI GKGCMC 1 cut(s) 17
BseX3I CGGCCG 1 cut(s) 366
BseXI GCAGC 5 cut(s) 146, 317, 409, 570, 638
Bsh1236I CGCG 2 cut(s) 302, 612
Bsh1285I CGRYCG 1 cut(s) 369
BshFI GGCC 3 cut(s) 336, 368, 477
BshTI ACCGGT 1 cut(s) 380
BsiEI CGRYCG 1 cut(s) 369
BsiSI CCGG 8 cut(s) 51, 381, 408, 545, 564, 653, 897, 921
BslFI GGGAC 5 cut(s) 238, 387, 562, 580, 802
BslI CCNNNNNNNGG 3 cut(s) 189, 484, 659
BsmAI GTCTC 2 cut(s) 240, 546
BsmBI CGTCTC 2 cut(s) 240, 546
BsmFI GGGAC 5 cut(s) 238, 387, 562, 580, 802
BsmI GAATGC 1 cut(s) 182
BsnI GGCC 3 cut(s) 336, 368, 477
Bsp1286I GDGCHC 1 cut(s) 17
Bsp143I GATC 4 cut(s) 115, 237, 492, 552
BspACI CCGC 8 cut(s) 213, 300, 302, 333, 369, 525, 587, 594
BspANI GGCC 3 cut(s) 336, 368, 477
BspCNI CTCAG 3 cut(s) 201, 402, 874
BspFNI CGCG 2 cut(s) 302, 612
BspLI GGNNCC 1 cut(s) 320
BspMAI CTGCAG 1 cut(s) 136
BspMI ACCTGC 1 cut(s) 790
BspPI GGATC 2 cut(s) 110, 500
BsrFI RCCGGY 1 cut(s) 380
BsrI ACTGG 1 cut(s) 769
BssAI RCCGGY 1 cut(s) 380
BssECI CCNNGG 3 cut(s) 300, 545, 653
BssMI GATC 4 cut(s) 115, 237, 492, 552
BssNI GRCGYC 1 cut(s) 891
Bst2UI CCWGG 1 cut(s) 485
Bst4CI ACNGT 2 cut(s) 571, 703
BstACI GRCGYC 1 cut(s) 891
BstC8I GCNNGC 1 cut(s) 373
BstDEI CTNAG 3 cut(s) 188, 389, 861
BstDSI CCRYGG 1 cut(s) 300
BstEII GGTNACC 1 cut(s) 383
BstF5I GGATG 1 cut(s) 664
BstFNI CGCG 2 cut(s) 302, 612
BstH2I RGCGCY 1 cut(s) 606
BstHHI GCGC 4 cut(s) 173, 182, 605, 612
BstKTI GATC 4 cut(s) 118, 240, 495, 555
BstMAI GTCTC 2 cut(s) 240, 546
BstMBI GATC 4 cut(s) 115, 237, 492, 552
BstMCI CGRYCG 1 cut(s) 369
BstMWI GCNNNNNNNGC 6 cut(s) 177, 179, 419, 602, 609, 623
BstNI CCWGG 1 cut(s) 485
BstNSI RCATGY 2 cut(s) 225, 678
BstPI GGTNACC 1 cut(s) 383
BstSCI CCNGG 5 cut(s) 483, 544, 563, 652, 919
BstSFI CTRYAG 1 cut(s) 132
BstSLI GKGCMC 1 cut(s) 17
BstUI CGCG 2 cut(s) 302, 612
BstV1I GCAGC 5 cut(s) 146, 317, 409, 570, 638
BstXI CCANNNNNNTGG 1 cut(s) 263
BstZI CGGCCG 1 cut(s) 366
BsuI GTATCC 1 cut(s) 720
BsuRI GGCC 3 cut(s) 336, 368, 477
BtgI CCRYGG 1 cut(s) 300
BtgZI GCGATG 1 cut(s) 644
BtsCI GGATG 1 cut(s) 664
BveI ACCTGC 1 cut(s) 790
Cac8I GCNNGC 1 cut(s) 373
CfoI GCGC 4 cut(s) 173, 182, 605, 612
Cfr10I RCCGGY 1 cut(s) 380
Cfr42I CCGCGG 1 cut(s) 303
CseI GACGC 2 cut(s) 623, 767
CsiI ACCWGGT 1 cut(s) 483
CspAI ACCGGT 1 cut(s) 380
CviAII CATG 4 cut(s) 162, 222, 356, 675
DdeI CTNAG 3 cut(s) 188, 389, 861
DpnI GATC 4 cut(s) 117, 239, 494, 554
DpnII GATC 4 cut(s) 115, 237, 492, 552
EaeI YGGCCR 2 cut(s) 334, 366
EagI CGGCCG 1 cut(s) 366
EclXI CGGCCG 1 cut(s) 366
Eco47III AGCGCT 1 cut(s) 604
Eco52I CGGCCG 1 cut(s) 366
Eco57I CTGAAG 2 cut(s) 583, 630
Eco91I GGTNACC 1 cut(s) 383
EcoO65I GGTNACC 1 cut(s) 383
EcoRII CCWGG 1 cut(s) 483
Esp3I CGTCTC 2 cut(s) 240, 546
FaeI CATG 4 cut(s) 165, 225, 359, 678
FalI AAGNNNNNCTT 4 cut(s) 275, 307, 628, 660
FaqI GGGAC 5 cut(s) 238, 387, 562, 580, 802
FatI CATG 4 cut(s) 161, 221, 355, 674
FauI CCCGC 2 cut(s) 307, 532
FauNDI CATATG 1 cut(s) 148
FblI GTMKAC 1 cut(s) 774
Fnu4HI GCNGC 9 cut(s) 135, 213, 331, 334, 369, 423, 584, 587, 627
FokI GGATG 1 cut(s) 671
Fsp4HI GCNGC 9 cut(s) 135, 213, 331, 334, 369, 423, 584, 587, 627
FspBI CTAG 1 cut(s) 668
GlaI GCGC 4 cut(s) 172, 181, 604, 611
GluI GCNGC 9 cut(s) 135, 213, 331, 334, 369, 423, 584, 587, 627
HaeII RGCGCY 1 cut(s) 606
HaeIII GGCC 3 cut(s) 336, 368, 477
HapII CCGG 8 cut(s) 51, 381, 408, 545, 564, 653, 897, 921
HgaI GACGC 2 cut(s) 623, 767
HhaI GCGC 4 cut(s) 173, 182, 605, 612
Hin1I GRCGYC 1 cut(s) 891
Hin1II CATG 4 cut(s) 165, 225, 359, 678
Hin6I GCGC 4 cut(s) 171, 180, 603, 610
HinP1I GCGC 4 cut(s) 171, 180, 603, 610
HincII GTYRAC 1 cut(s) 750
HindII GTYRAC 1 cut(s) 750
HindIII AAGCTT 2 cut(s) 55, 512
HinfI GANTC 6 cut(s) 70, 251, 560, 663, 768, 906
HpaII CCGG 8 cut(s) 51, 381, 408, 545, 564, 653, 897, 921
HphI GGTGA 2 cut(s) 247, 715
Hpy166II GTNNAC 3 cut(s) 502, 750, 775
Hpy188I TCNGA 3 cut(s) 714, 854, 911
Hpy188III TCNNGA 1 cut(s) 190
Hpy8I GTNNAC 3 cut(s) 502, 750, 775
Hpy99I CGWCG 3 cut(s) 617, 740, 896
HpyAV CCTTC 3 cut(s) 341, 607, 654
HpyCH4III ACNGT 2 cut(s) 571, 703
HpyCH4IV ACGT 5 cut(s) 306, 467, 707, 746, 891
HpyCH4V TGCA 3 cut(s) 134, 693, 833
HpyF10VI GCNNNNNNNGC 6 cut(s) 177, 179, 419, 602, 609, 623
HpyF3I CTNAG 3 cut(s) 188, 389, 861
HpySE526I ACGT 5 cut(s) 306, 467, 707, 746, 891
Hsp92I GRCGYC 1 cut(s) 891
Hsp92II CATG 4 cut(s) 165, 225, 359, 678
HspAI GCGC 4 cut(s) 171, 180, 603, 610
KspI CCGCGG 1 cut(s) 303
Kzo9I GATC 4 cut(s) 115, 237, 492, 552
LmnI GCTCC 3 cut(s) 380, 410, 922
Lsp1109I GCAGC 5 cut(s) 146, 317, 409, 570, 638
LweI GCATC 2 cut(s) 65, 820
MabI ACCWGGT 1 cut(s) 483
MaeI CTAG 1 cut(s) 668
MaeII ACGT 5 cut(s) 306, 467, 707, 746, 891
MaeIII GTNAC 4 cut(s) 164, 383, 703, 742
MalI GATC 4 cut(s) 117, 239, 494, 554
MboI GATC 4 cut(s) 115, 237, 492, 552
MboII GAAGA 1 cut(s) 880
MhlI GDGCHC 1 cut(s) 17
MluCI AATT 3 cut(s) 41, 156, 519
MlyI GAGTC 4 cut(s) 64, 260, 554, 915
MmeI TCCRAC 2 cut(s) 722, 889
MnlI CCTC 9 cut(s) 71, 148, 154, 226, 332, 398, 466, 695, 871
MseI TTAA 2 cut(s) 293, 480
MslI CAYNNNNRTG 1 cut(s) 261
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 8 cut(s) 51, 381, 408, 545, 564, 653, 897, 921
MspR9I CCNGG 5 cut(s) 485, 546, 565, 654, 921
Mva1269I GAATGC 1 cut(s) 182
MvaI CCWGG 1 cut(s) 485
MvnI CGCG 2 cut(s) 302, 612
MwoI GCNNNNNNNGC 6 cut(s) 177, 179, 419, 602, 609, 623
NciI CCSGG 4 cut(s) 546, 565, 654, 921
NdeI CATATG 1 cut(s) 148
NdeII GATC 4 cut(s) 115, 237, 492, 552
NlaIII CATG 4 cut(s) 165, 225, 359, 678
NlaIV GGNNCC 1 cut(s) 320
NmeAIII GCCGAG 1 cut(s) 344
NmuCI GTSAC 1 cut(s) 703
NspI RCATGY 2 cut(s) 225, 678
PcsI WCGNNNNNNNCGW 1 cut(s) 536
PctI GAATGC 1 cut(s) 182
PfeI GAWTC 2 cut(s) 663, 768
PfoI TCCNGGA 1 cut(s) 563
PinAI ACCGGT 1 cut(s) 380
PkrI GCNGC 9 cut(s) 136, 214, 332, 335, 370, 424, 585, 588, 628
PleI GAGTC 4 cut(s) 64, 259, 554, 914
PpsI GAGTC 4 cut(s) 64, 259, 554, 914
Psp6I CCWGG 1 cut(s) 483
PspEI GGTNACC 1 cut(s) 383
PspGI CCWGG 1 cut(s) 483
PspN4I GGNNCC 1 cut(s) 320
PstI CTGCAG 1 cut(s) 136
RseI CAYNNNNRTG 1 cut(s) 261
SacII CCGCGG 1 cut(s) 303
SaqAI TTAA 2 cut(s) 293, 480
SatI GCNGC 9 cut(s) 135, 213, 331, 334, 369, 423, 584, 587, 627
Sau3AI GATC 4 cut(s) 115, 237, 492, 552
SchI GAGTC 4 cut(s) 64, 260, 554, 915
ScrFI CCNGG 5 cut(s) 485, 546, 565, 654, 921
SduI GDGCHC 1 cut(s) 17
SexAI ACCWGGT 1 cut(s) 483
SfaNI GCATC 2 cut(s) 65, 820
SfcI CTRYAG 1 cut(s) 132
Sfr303I CCGCGG 1 cut(s) 303
SgrBI CCGCGG 1 cut(s) 303
SmiMI CAYNNNNRTG 1 cut(s) 261
Sse9I AATT 3 cut(s) 41, 156, 519
SsiI CCGC 8 cut(s) 213, 300, 302, 333, 369, 525, 587, 594
SspMI CTAG 1 cut(s) 668
StyD4I CCNGG 5 cut(s) 483, 544, 563, 652, 919
TaaI ACNGT 2 cut(s) 571, 703
TaiI ACGT 5 cut(s) 309, 470, 710, 749, 894
TaqI TCGA 3 cut(s) 39, 73, 126
TasI AATT 3 cut(s) 41, 156, 519
TauI GCSGC 4 cut(s) 215, 336, 371, 589
TfiI GAWTC 2 cut(s) 663, 768
Tru1I TTAA 2 cut(s) 293, 480
Tru9I TTAA 2 cut(s) 293, 480
TseFI GTSAC 1 cut(s) 703
TseI GCWGC 5 cut(s) 134, 330, 422, 583, 626
Tsp45I GTSAC 1 cut(s) 703
TspDTI ATGAA 2 cut(s) 150, 809
XapI RAATTY 2 cut(s) 41, 156
XceI RCATGY 2 cut(s) 225, 678
XmiI GTMKAC 1 cut(s) 774
XspI CTAG 1 cut(s) 668
ZraI GACGTC 1 cut(s) 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.