Belongs to the peptidase A1 family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 69, 325 |
| AatII | GACGTC | 1 cut(s) | 1593 |
| Acc36I | ACCTGC | 4 cut(s) | 69, 325, 1029, 1489 |
| AccBSI | CCGCTC | 1 cut(s) | 296 |
| AccI | GTMKAC | 1 cut(s) | 1473 |
| AccII | CGCG | 5 cut(s) | 484, 996, 1025, 1311, 1316 |
| AclWI | GGATC | 3 cut(s) | 126, 716, 1199 |
| AcoI | YGGCCR | 2 cut(s) | 940, 972 |
| AcsI | RAATTY | 4 cut(s) | 196, 462, 650, 762 |
| AcuI | CTGAAG | 3 cut(s) | 1212, 1282, 1329 |
| AcyI | GRCGYC | 1 cut(s) | 1590 |
| AdeI | CACNNNGTG | 1 cut(s) | 439 |
| AfaI | GTAC | 3 cut(s) | 399, 1004, 1034 |
| AfeI | AGCGCT | 1 cut(s) | 1303 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 550, 795, 838, 1183, 1358 |
| AflIII | ACRYGT | 1 cut(s) | 1314 |
| AgeI | ACCGGT | 1 cut(s) | 1079 |
| AgsI | TTSAA | 2 cut(s) | 248, 1218 |
| AjnI | CCWGG | 4 cut(s) | 960, 1182, 1365, 1618 |
| AluBI | AGCT | 9 cut(s) | 20, 666, 743, 892, 1074, 1112, 1161, 1213, 1338 |
| AluI | AGCT | 9 cut(s) | 20, 666, 743, 892, 1074, 1112, 1161, 1213, 1338 |
| Alw26I | GTCTC | 4 cut(s) | 262, 846, 992, 1245 |
| AlwI | GGATC | 3 cut(s) | 126, 716, 1199 |
| Ama87I | CYCGRG | 2 cut(s) | 218, 467 |
| Aor51HI | AGCGCT | 1 cut(s) | 1303 |
| AoxI | GGCC | 6 cut(s) | 410, 541, 552, 940, 972, 1174 |
| ApeKI | GCWGC | 3 cut(s) | 176, 740, 936 |
| ApoI | RAATTY | 4 cut(s) | 196, 462, 650, 762 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 899, 931 |
| AseI | ATTAAT | 1 cut(s) | 123 |
| AsiGI | ACCGGT | 1 cut(s) | 1079 |
| AspLEI | GCGC | 5 cut(s) | 484, 779, 788, 1304, 1311 |
| AspS9I | GGNCC | 1 cut(s) | 410 |
| AsuC2I | CCSGG | 4 cut(s) | 571, 1245, 1264, 1353 |
| AsuHPI | GGTGA | 3 cut(s) | 853, 1081, 1414 |
| AvaI | CYCGRG | 2 cut(s) | 218, 467 |
| BaeGI | GKGCMC | 1 cut(s) | 626 |
| BbsI | GAAGAC | 2 cut(s) | 162, 386 |
| BbvI | GCAGC | 3 cut(s) | 163, 752, 923 |
| BccI | CCATC | 3 cut(s) | 370, 914, 1352 |
| BceAI | ACGGC | 6 cut(s) | 541, 982, 1051, 1154, 1189, 1295 |
| BciT130I | CCWGG | 4 cut(s) | 962, 1184, 1367, 1620 |
| BciVI | GTATCC | 2 cut(s) | 1363, 1419 |
| BcnI | CCSGG | 4 cut(s) | 571, 1245, 1264, 1353 |
| BcoDI | GTCTC | 4 cut(s) | 262, 846, 992, 1245 |
| BfaI | CTAG | 2 cut(s) | 539, 1158 |
| BfmI | CTRYAG | 1 cut(s) | 1008 |
| BfoI | RGCGCY | 1 cut(s) | 1305 |
| BfuAI | ACCTGC | 4 cut(s) | 69, 325, 1029, 1489 |
| BfuI | GTATCC | 2 cut(s) | 1363, 1419 |
| BisI | GCNGC | 6 cut(s) | 177, 741, 937, 940, 975, 1122 |
| BlsI | GCNGC | 6 cut(s) | 178, 742, 938, 941, 976, 1123 |
| BmcAI | AGTACT | 1 cut(s) | 1004 |
| Bme1390I | CCNGG | 8 cut(s) | 571, 962, 1184, 1245, 1264, 1353, 1367, 1620 |
| BmeT110I | CYCGRG | 2 cut(s) | 218, 467 |
| BmgT120I | GGNCC | 1 cut(s) | 410 |
| BmiI | GGNNCC | 1 cut(s) | 926 |
| BmrFI | CCNGG | 8 cut(s) | 571, 962, 1184, 1245, 1264, 1353, 1367, 1620 |
| BmsI | GCATC | 1 cut(s) | 1519 |
| BpiI | GAAGAC | 2 cut(s) | 162, 386 |
| BpmI | CTGGAG | 1 cut(s) | 1602 |
| BpuMI | CCSGG | 4 cut(s) | 571, 1245, 1264, 1353 |
| BsaHI | GRCGYC | 1 cut(s) | 1590 |
| BsaI | GGTCTC | 1 cut(s) | 992 |
| BsaJI | CCNNGG | 4 cut(s) | 219, 1023, 1244, 1352 |
| BsaWI | WCCGGW | 3 cut(s) | 659, 1079, 1106 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 886, 916, 1568, 1590, 1598, 1620 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 550, 795, 838, 1183, 1358 |
| Bse118I | RCCGGY | 1 cut(s) | 1079 |
| Bse1I | ACTGG | 2 cut(s) | 399, 1468 |
| Bse3DI | GCAATG | 2 cut(s) | 99, 535 |
| BseBI | CCWGG | 4 cut(s) | 962, 1184, 1367, 1620 |
| BseDI | CCNNGG | 4 cut(s) | 219, 1023, 1244, 1352 |
| BseGI | GGATG | 1 cut(s) | 1363 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 550, 795, 838, 1183, 1358 |
| BseMI | GCAATG | 2 cut(s) | 99, 535 |
| BseMII | CTCAG | 3 cut(s) | 808, 1399, 1574 |
| BseNI | ACTGG | 2 cut(s) | 399, 1468 |
| BseRI | GAGGAG | 1 cut(s) | 112 |
| BseSI | GKGCMC | 1 cut(s) | 626 |
| BseX3I | CGGCCG | 1 cut(s) | 972 |
| BseXI | GCAGC | 3 cut(s) | 163, 752, 923 |
| BsgI | GTGCAG | 1 cut(s) | 612 |
| Bsh1236I | CGCG | 5 cut(s) | 484, 996, 1025, 1311, 1316 |
| Bsh1285I | CGRYCG | 2 cut(s) | 975, 1068 |
| BshFI | GGCC | 6 cut(s) | 412, 543, 554, 942, 974, 1176 |
| BshTI | ACCGGT | 1 cut(s) | 1079 |
| BsiEI | CGRYCG | 2 cut(s) | 975, 1068 |
| BsiHKCI | CYCGRG | 2 cut(s) | 218, 467 |
| BsiSI | CCGG | 8 cut(s) | 571, 660, 1080, 1107, 1244, 1263, 1352, 1596 |
| BslFI | GGGAC | 5 cut(s) | 553, 1086, 1261, 1279, 1501 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 550, 795, 838, 1183, 1358 |
| BsmAI | GTCTC | 4 cut(s) | 262, 846, 992, 1245 |
| BsmBI | CGTCTC | 3 cut(s) | 262, 846, 1245 |
| BsmFI | GGGAC | 5 cut(s) | 553, 1086, 1261, 1279, 1501 |
| BsmI | GAATGC | 1 cut(s) | 788 |
| BsnI | GGCC | 6 cut(s) | 412, 543, 554, 942, 974, 1176 |
| Bso31I | GGTCTC | 1 cut(s) | 992 |
| BsoBI | CYCGRG | 2 cut(s) | 218, 467 |
| Bsp1286I | GDGCHC | 1 cut(s) | 626 |
| Bsp143I | GATC | 7 cut(s) | 64, 131, 288, 535, 721, 843, 1191 |
| BspANI | GGCC | 6 cut(s) | 412, 543, 554, 942, 974, 1176 |
| BspCNI | CTCAG | 3 cut(s) | 807, 1398, 1573 |
| BspFNI | CGCG | 5 cut(s) | 484, 996, 1025, 1311, 1316 |
| BspHI | TCATGA | 1 cut(s) | 264 |
| BspLI | GGNNCC | 1 cut(s) | 926 |
| BspMI | ACCTGC | 4 cut(s) | 69, 325, 1029, 1489 |
| BspPI | GGATC | 3 cut(s) | 126, 716, 1199 |
| BspTNI | GGTCTC | 1 cut(s) | 992 |
| BsrBI | CCGCTC | 1 cut(s) | 296 |
| BsrDI | GCAATG | 2 cut(s) | 99, 535 |
| BsrFI | RCCGGY | 1 cut(s) | 1079 |
| BsrI | ACTGG | 2 cut(s) | 399, 1468 |
| BssAI | RCCGGY | 1 cut(s) | 1079 |
| BssECI | CCNNGG | 4 cut(s) | 219, 1023, 1244, 1352 |
| BssMI | GATC | 7 cut(s) | 64, 131, 288, 535, 721, 843, 1191 |
| BssNI | GRCGYC | 1 cut(s) | 1590 |
| Bst2UI | CCWGG | 4 cut(s) | 962, 1184, 1367, 1620 |
| Bst4CI | ACNGT | 6 cut(s) | 34, 285, 613, 1055, 1270, 1402 |
| BstACI | GRCGYC | 1 cut(s) | 1590 |
| BstC8I | GCNNGC | 4 cut(s) | 147, 308, 595, 1072 |
| BstDEI | CTNAG | 4 cut(s) | 230, 794, 1385, 1560 |
| BstDSI | CCRYGG | 1 cut(s) | 1023 |
| BstEII | GGTNACC | 1 cut(s) | 1082 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 836 |
| BstF5I | GGATG | 1 cut(s) | 1363 |
| BstFNI | CGCG | 5 cut(s) | 484, 996, 1025, 1311, 1316 |
| BstH2I | RGCGCY | 1 cut(s) | 1305 |
| BstHHI | GCGC | 5 cut(s) | 484, 779, 788, 1304, 1311 |
| BstKTI | GATC | 7 cut(s) | 67, 134, 291, 538, 724, 846, 1194 |
| BstMAI | GTCTC | 4 cut(s) | 262, 846, 992, 1245 |
| BstMBI | GATC | 7 cut(s) | 64, 131, 288, 535, 721, 843, 1191 |
| BstMCI | CGRYCG | 2 cut(s) | 975, 1068 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 783, 785, 1118, 1301, 1308 |
| BstNI | CCWGG | 4 cut(s) | 962, 1184, 1367, 1620 |
| BstNSI | RCATGY | 3 cut(s) | 116, 831, 1377 |
| BstPI | GGTNACC | 1 cut(s) | 1082 |
| BstSCI | CCNGG | 8 cut(s) | 569, 960, 1182, 1243, 1262, 1351, 1365, 1618 |
| BstSFI | CTRYAG | 1 cut(s) | 1008 |
| BstSLI | GKGCMC | 1 cut(s) | 626 |
| BstUI | CGCG | 5 cut(s) | 484, 996, 1025, 1311, 1316 |
| BstV1I | GCAGC | 3 cut(s) | 163, 752, 923 |
| BstV2I | GAAGAC | 2 cut(s) | 162, 386 |
| BstX2I | RGATCY | 1 cut(s) | 131 |
| BstYI | RGATCY | 1 cut(s) | 131 |
| BstZI | CGGCCG | 1 cut(s) | 972 |
| BsuI | GTATCC | 2 cut(s) | 1363, 1419 |
| BsuRI | GGCC | 6 cut(s) | 412, 543, 554, 942, 974, 1176 |
| BtgI | CCRYGG | 1 cut(s) | 1023 |
| BtsCI | GGATG | 1 cut(s) | 1363 |
| BtsIMutI | CAGTG | 3 cut(s) | 39, 281, 1393 |
| BveI | ACCTGC | 4 cut(s) | 69, 325, 1029, 1489 |
| Cac8I | GCNNGC | 4 cut(s) | 147, 308, 595, 1072 |
| CciI | TCATGA | 1 cut(s) | 264 |
| CfoI | GCGC | 5 cut(s) | 484, 779, 788, 1304, 1311 |
| Cfr10I | RCCGGY | 1 cut(s) | 1079 |
| Cfr13I | GGNCC | 1 cut(s) | 410 |
| Cfr42I | CCGCGG | 1 cut(s) | 1026 |
| CseI | GACGC | 2 cut(s) | 1322, 1466 |
| CsiI | ACCWGGT | 1 cut(s) | 1182 |
| Csp6I | GTAC | 3 cut(s) | 398, 1003, 1033 |
| CspAI | ACCGGT | 1 cut(s) | 1079 |
| CviAII | CATG | 9 cut(s) | 53, 58, 113, 265, 407, 414, 768, 828, 1374 |
| CviQI | GTAC | 3 cut(s) | 398, 1003, 1033 |
| DdeI | CTNAG | 4 cut(s) | 230, 794, 1385, 1560 |
| DpnI | GATC | 7 cut(s) | 66, 133, 290, 537, 723, 845, 1193 |
| DpnII | GATC | 7 cut(s) | 64, 131, 288, 535, 721, 843, 1191 |
| DraIII | CACNNNGTG | 1 cut(s) | 439 |
| EaeI | YGGCCR | 2 cut(s) | 940, 972 |
| EagI | CGGCCG | 1 cut(s) | 972 |
| EclXI | CGGCCG | 1 cut(s) | 972 |
| Eco31I | GGTCTC | 1 cut(s) | 992 |
| Eco47III | AGCGCT | 1 cut(s) | 1303 |
| Eco52I | CGGCCG | 1 cut(s) | 972 |
| Eco57I | CTGAAG | 3 cut(s) | 1212, 1282, 1329 |
| Eco88I | CYCGRG | 2 cut(s) | 218, 467 |
| Eco91I | GGTNACC | 1 cut(s) | 1082 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 836 |
| EcoO65I | GGTNACC | 1 cut(s) | 1082 |
| EcoRI | GAATTC | 1 cut(s) | 196 |
| EcoRII | CCWGG | 4 cut(s) | 960, 1182, 1365, 1618 |
| Esp3I | CGTCTC | 3 cut(s) | 262, 846, 1245 |
| FaeI | CATG | 9 cut(s) | 56, 61, 116, 268, 410, 417, 771, 831, 1377 |
| FalI | AAGNNNNNCTT | 5 cut(s) | 36, 881, 913, 1327, 1359 |
| FaqI | GGGAC | 5 cut(s) | 553, 1086, 1261, 1279, 1501 |
| FatI | CATG | 9 cut(s) | 52, 57, 112, 264, 406, 413, 767, 827, 1373 |
| FauI | CCCGC | 3 cut(s) | 315, 745, 1001 |
| FblI | GTMKAC | 1 cut(s) | 1473 |
| Fnu4HI | GCNGC | 6 cut(s) | 177, 741, 937, 940, 975, 1122 |
| FokI | GGATG | 1 cut(s) | 1370 |
| Fsp4HI | GCNGC | 6 cut(s) | 177, 741, 937, 940, 975, 1122 |
| FspBI | CTAG | 2 cut(s) | 539, 1158 |
| GlaI | GCGC | 5 cut(s) | 483, 778, 787, 1303, 1310 |
| GluI | GCNGC | 6 cut(s) | 177, 741, 937, 940, 975, 1122 |
| GsuI | CTGGAG | 1 cut(s) | 1602 |
| HaeII | RGCGCY | 1 cut(s) | 1305 |
| HaeIII | GGCC | 6 cut(s) | 412, 543, 554, 942, 974, 1176 |
| HapII | CCGG | 8 cut(s) | 571, 660, 1080, 1107, 1244, 1263, 1352, 1596 |
| HgaI | GACGC | 2 cut(s) | 1322, 1466 |
| HhaI | GCGC | 5 cut(s) | 484, 779, 788, 1304, 1311 |
| Hin1I | GRCGYC | 1 cut(s) | 1590 |
| Hin1II | CATG | 9 cut(s) | 56, 61, 116, 268, 410, 417, 771, 831, 1377 |
| Hin6I | GCGC | 5 cut(s) | 482, 777, 786, 1302, 1309 |
| HinP1I | GCGC | 5 cut(s) | 482, 777, 786, 1302, 1309 |
| HincII | GTYRAC | 3 cut(s) | 339, 490, 1449 |
| HindII | GTYRAC | 3 cut(s) | 339, 490, 1449 |
| HindIII | AAGCTT | 3 cut(s) | 18, 664, 1211 |
| HpaI | GTTAAC | 1 cut(s) | 339 |
| HpaII | CCGG | 8 cut(s) | 571, 660, 1080, 1107, 1244, 1263, 1352, 1596 |
| HphI | GGTGA | 3 cut(s) | 853, 1081, 1414 |
| Hpy166II | GTNNAC | 6 cut(s) | 339, 400, 490, 1201, 1449, 1474 |
| Hpy188I | TCNGA | 6 cut(s) | 202, 550, 1231, 1413, 1553, 1610 |
| Hpy188III | TCNNGA | 6 cut(s) | 265, 389, 467, 796, 905, 1543 |
| Hpy8I | GTNNAC | 6 cut(s) | 339, 400, 490, 1201, 1449, 1474 |
| Hpy99I | CGWCG | 5 cut(s) | 911, 914, 1316, 1439, 1595 |
| HpyAV | CCTTC | 6 cut(s) | 145, 235, 271, 1102, 1306, 1353 |
| HpyCH4III | ACNGT | 6 cut(s) | 34, 285, 613, 1055, 1270, 1402 |
| HpyCH4IV | ACGT | 6 cut(s) | 255, 345, 912, 951, 1406, 1590 |
| HpyCH4V | TGCA | 6 cut(s) | 8, 78, 145, 320, 593, 1532 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 783, 785, 1118, 1301, 1308 |
| HpyF3I | CTNAG | 4 cut(s) | 230, 794, 1385, 1560 |
| HpySE526I | ACGT | 6 cut(s) | 255, 345, 912, 951, 1406, 1590 |
| Hsp92I | GRCGYC | 1 cut(s) | 1590 |
| Hsp92II | CATG | 9 cut(s) | 56, 61, 116, 268, 410, 417, 771, 831, 1377 |
| HspAI | GCGC | 5 cut(s) | 482, 777, 786, 1302, 1309 |
| KspAI | GTTAAC | 1 cut(s) | 339 |
| KspI | CCGCGG | 1 cut(s) | 1026 |
| Kzo9I | GATC | 7 cut(s) | 64, 131, 288, 535, 721, 843, 1191 |
| LmnI | GCTCC | 3 cut(s) | 1079, 1109, 1621 |
| Lsp1109I | GCAGC | 3 cut(s) | 163, 752, 923 |
| LweI | GCATC | 1 cut(s) | 1519 |
| MabI | ACCWGGT | 1 cut(s) | 1182 |
| MaeI | CTAG | 2 cut(s) | 539, 1158 |
| MaeII | ACGT | 6 cut(s) | 255, 345, 912, 951, 1406, 1590 |
| MaeIII | GTNAC | 8 cut(s) | 28, 86, 439, 607, 770, 1082, 1402, 1540 |
| MalI | GATC | 7 cut(s) | 66, 133, 290, 537, 723, 845, 1193 |
| MbiI | CCGCTC | 1 cut(s) | 296 |
| MboI | GATC | 7 cut(s) | 64, 131, 288, 535, 721, 843, 1191 |
| MboII | GAAGA | 6 cut(s) | 156, 162, 220, 391, 416, 1579 |
| MflI | RGATCY | 1 cut(s) | 131 |
| MhlI | GDGCHC | 1 cut(s) | 626 |
| MluCI | AATT | 7 cut(s) | 196, 349, 462, 650, 762, 1218, 1254 |
| MluI | ACGCGT | 1 cut(s) | 1314 |
| MlyI | GAGTC | 5 cut(s) | 495, 673, 866, 1253, 1614 |
| MmeI | TCCRAC | 3 cut(s) | 22, 1421, 1588 |
| MseI | TTAA | 6 cut(s) | 105, 123, 338, 348, 899, 1179 |
| MslI | CAYNNNNRTG | 1 cut(s) | 405 |
| MspA1I | CMGCKG | 1 cut(s) | 1025 |
| MspI | CCGG | 8 cut(s) | 571, 660, 1080, 1107, 1244, 1263, 1352, 1596 |
| MspR9I | CCNGG | 8 cut(s) | 571, 962, 1184, 1245, 1264, 1353, 1367, 1620 |
| Mva1269I | GAATGC | 1 cut(s) | 788 |
| MvaI | CCWGG | 4 cut(s) | 962, 1184, 1367, 1620 |
| MvnI | CGCG | 5 cut(s) | 484, 996, 1025, 1311, 1316 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 783, 785, 1118, 1301, 1308 |
| NciI | CCSGG | 4 cut(s) | 571, 1245, 1264, 1353 |
| NdeII | GATC | 7 cut(s) | 64, 131, 288, 535, 721, 843, 1191 |
| NlaIII | CATG | 9 cut(s) | 56, 61, 116, 268, 410, 417, 771, 831, 1377 |
| NlaIV | GGNNCC | 1 cut(s) | 926 |
| NmeAIII | GCCGAG | 1 cut(s) | 950 |
| NmuCI | GTSAC | 5 cut(s) | 28, 439, 607, 1402, 1540 |
| NspI | RCATGY | 3 cut(s) | 116, 831, 1377 |
| PaeR7I | CTCGAG | 1 cut(s) | 467 |
| PagI | TCATGA | 1 cut(s) | 264 |
| PaqCI | CACCTGC | 2 cut(s) | 69, 325 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 915 |
| PctI | GAATGC | 1 cut(s) | 788 |
| PfeI | GAWTC | 7 cut(s) | 154, 211, 224, 358, 451, 1362, 1467 |
| PfoI | TCCNGGA | 3 cut(s) | 960, 1262, 1365 |
| PinAI | ACCGGT | 1 cut(s) | 1079 |
| PkrI | GCNGC | 6 cut(s) | 178, 742, 938, 941, 976, 1123 |
| PleI | GAGTC | 5 cut(s) | 494, 673, 865, 1253, 1613 |
| PpsI | GAGTC | 5 cut(s) | 494, 673, 865, 1253, 1613 |
| PshBI | ATTAAT | 1 cut(s) | 123 |
| Psp6I | CCWGG | 4 cut(s) | 960, 1182, 1365, 1618 |
| PspEI | GGTNACC | 1 cut(s) | 1082 |
| PspGI | CCWGG | 4 cut(s) | 960, 1182, 1365, 1618 |
| PspN4I | GGNNCC | 1 cut(s) | 926 |
| PspPI | GGNCC | 1 cut(s) | 410 |
| PsuI | RGATCY | 1 cut(s) | 131 |
| RsaI | GTAC | 3 cut(s) | 399, 1004, 1034 |
| RsaNI | GTAC | 3 cut(s) | 398, 1003, 1033 |
| RseI | CAYNNNNRTG | 1 cut(s) | 405 |
| SacII | CCGCGG | 1 cut(s) | 1026 |
| SaqAI | TTAA | 6 cut(s) | 105, 123, 338, 348, 899, 1179 |
| SatI | GCNGC | 6 cut(s) | 177, 741, 937, 940, 975, 1122 |
| Sau3AI | GATC | 7 cut(s) | 64, 131, 288, 535, 721, 843, 1191 |
| Sau96I | GGNCC | 1 cut(s) | 410 |
| ScaI | AGTACT | 1 cut(s) | 1004 |
| SchI | GAGTC | 5 cut(s) | 495, 673, 866, 1253, 1614 |
| ScrFI | CCNGG | 8 cut(s) | 571, 962, 1184, 1245, 1264, 1353, 1367, 1620 |
| SduI | GDGCHC | 1 cut(s) | 626 |
| SexAI | ACCWGGT | 1 cut(s) | 1182 |
| SfaNI | GCATC | 1 cut(s) | 1519 |
| SfcI | CTRYAG | 1 cut(s) | 1008 |
| Sfr274I | CTCGAG | 1 cut(s) | 467 |
| Sfr303I | CCGCGG | 1 cut(s) | 1026 |
| SgrBI | CCGCGG | 1 cut(s) | 1026 |
| SlaI | CTCGAG | 1 cut(s) | 467 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 405 |
| SmlI | CTYRAG | 1 cut(s) | 467 |
| SmoI | CTYRAG | 1 cut(s) | 467 |
| Sse9I | AATT | 7 cut(s) | 196, 349, 462, 650, 762, 1218, 1254 |
| SspMI | CTAG | 2 cut(s) | 539, 1158 |
| StyD4I | CCNGG | 8 cut(s) | 569, 960, 1182, 1243, 1262, 1351, 1365, 1618 |
| TaaI | ACNGT | 6 cut(s) | 34, 285, 613, 1055, 1270, 1402 |
| TaiI | ACGT | 6 cut(s) | 258, 348, 915, 954, 1409, 1593 |
| TaqI | TCGA | 6 cut(s) | 186, 468, 648, 732, 918, 1014 |
| TasI | AATT | 7 cut(s) | 196, 349, 462, 650, 762, 1218, 1254 |
| TatI | WGTACW | 1 cut(s) | 1002 |
| TauI | GCSGC | 3 cut(s) | 942, 977, 1124 |
| TfiI | GAWTC | 7 cut(s) | 154, 211, 224, 358, 451, 1362, 1467 |
| Tru1I | TTAA | 6 cut(s) | 105, 123, 338, 348, 899, 1179 |
| Tru9I | TTAA | 6 cut(s) | 105, 123, 338, 348, 899, 1179 |
| TscAI | CASTG | 3 cut(s) | 39, 288, 1393 |
| TseFI | GTSAC | 5 cut(s) | 28, 439, 607, 1402, 1540 |
| TseI | GCWGC | 3 cut(s) | 176, 740, 936 |
| Tsp45I | GTSAC | 5 cut(s) | 28, 439, 607, 1402, 1540 |
| TspDTI | ATGAA | 8 cut(s) | 41, 74, 209, 253, 281, 756, 1346, 1508 |
| TspRI | CASTG | 3 cut(s) | 39, 288, 1393 |
| VspI | ATTAAT | 1 cut(s) | 123 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 836 |
| XapI | RAATTY | 4 cut(s) | 196, 462, 650, 762 |
| XceI | RCATGY | 3 cut(s) | 116, 831, 1377 |
| XhoI | CTCGAG | 1 cut(s) | 467 |
| XmiI | GTMKAC | 1 cut(s) | 1473 |
| XspI | CTAG | 2 cut(s) | 539, 1158 |
| ZraI | GACGTC | 1 cut(s) | 1591 |
| ZrmI | AGTACT | 1 cut(s) | 1004 |