Rroxscaffold_5G00347230

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000845
Physical Location & Seq
Forward (+)
18521627 .. 18524262
2636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00347230.1

Sequence Viewer

Length: 1416 bp
ATGAGGAATTTCATCAGTTTATCTCTATTTCTATGCCTGACTCTCTGCCTTTTGGACAAAGGCTTTGCCTTGGCAGAAGCAGATGCCCACACAGTTGATCTCCGCTCTCTACTGCCAGCAACGACCTGCAGCCCCTCCACCAAAGGTAATAAGAGGAAGGCATCTCTTGAAGTGGTACACAAACATGGGCCATGCTCTCATCTCGCTCAACACAAAGCGACTGCTATGAATCACACTCAAATTCTAGAGCAAGACCAAGTGCGAGTGAACTCAATCAACTCCCGAATTTTCAAGAAATTGAAAGGCAACGAGGATATAAGCCAATCAGAGGCCACCAGCCTCCCGGCCCGGTGGGCTACCAGCCAAGGTGCAAGCAACTACGTGGTGACAGTGCGATTGGGCACACCTAACAAACAACTTTCGCTTGTATTTGACACCGGAAGCTATCTCACTTGGACTCAATGCCAACCTTGTGCTGGCTCTTGCTACCCACAAAAGGAGCCTATTTTCGATCCCTCAGCCTCCACCTCATATACCAACATTTCCTGTAATAGCATAGACTGCATCCAACTCAGGACAACTACAAATTTCAACAGATGCTTAGCAGACACATGCTTATACGCCGTGGGGTATGGAGACTCATCCTACACCATTGGATTATTCGCCACAGACAAGCTGACCTTAACTCCAAACGACGTGTTCAATGGGTTCTTCTTTGGCTGCGGCCAACAAAACGAAGGGTTCATCAATGGCACGGCCGGTATACTTGGTCTCTCCCGCAACAAGTTCTCCATCATTGAACAAACTATACAAAAGTACAACCGCTTCTTCTCCTACTGTCTGCCACCAACCGCAAGCTCTACTGGTTACCTCAGCTTCGGCGCTCCCAATGGAGCTTCCAATGCTGCTGTAAAATACACACGGCTCACTACTTTATCCTTGAACTCGGAGTTTTACGGCATAGATGTGGTTGGCATCAATGTGAACGGAAAAAAGCTCCCAATTCCTGCTTCGGTGTTTTCATCCCCGGGGACGATCATCGACTCCGGAACCGTCATCTCGCGACTGCCATCCACAGCGTATACCGCTTTACGGGATGCATTTCGCGCAGCGATGACAAGCTATCCGTTAGTTCCGGGGCCGGGTGAGCTCCTAGATACATGCTACGACCTTAGTAGCTATCCAAAGGTGATGTATCCGAAAGTAGAGTTTGTGTTCGGCGACGGGGTTACGTTAGAATTGGACGCAACCGGAATTGTCTACTTTCTCAACGATTTGAAGCAGGTTTGCTTGGCTTTTGCTGGGAATGATGATCCTAACGACATTGCGATTTTCGGGAATACTCAGCAGAAGAATATGGAGGTGTTGTATGACGTTGCCGGAGGGAGACTCGGATTTGCTCCCGGTGGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

471

Amino Acids

50.95

Weight (kDa)

6.95

Isoelectric Point (pI)

30.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 126 - 468 7.4e-23 Eukaryotic aspartyl protease
TAXi_N PF14543 127 - 294 5.1e-50 Xylanase inhibitor N-terminal
TAXi_C PF14541 319 - 467 1.9e-28 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 134, 1273
AccBSI CCGCTC 1 cut(s) 105
AccI GTMKAC 3 cut(s) 763, 1082, 1260
AccII CGCG 2 cut(s) 1063, 1107
AccIII TCCGGA 1 cut(s) 1046
AciI CCGC 6 cut(s) 103, 723, 778, 823, 852, 1086
AclWI GGATC 2 cut(s) 506, 1307
AcoI YGGCCR 2 cut(s) 724, 756
AcsI RAATTY 4 cut(s) 7, 240, 285, 586
AfaI GTAC 2 cut(s) 177, 818
AfiI CCNNNNNNNGG 5 cut(s) 328, 476, 496, 1092, 1142
AflIII ACRYGT 1 cut(s) 696
AgsI TTSAA 8 cut(s) 170, 292, 301, 592, 703, 800, 943, 1279
AjiI CACGTC 1 cut(s) 697
AjuI GAANNNNNNNTTGG 2 cut(s) 994, 1026
AluBI AGCT 9 cut(s) 444, 676, 858, 876, 896, 997, 1122, 1150, 1179
AluI AGCT 9 cut(s) 444, 676, 858, 876, 896, 997, 1122, 1150, 1179
Alw21I GWGCWC 1 cut(s) 1152
Alw26I GTCTC 3 cut(s) 630, 776, 1381
AlwI GGATC 2 cut(s) 506, 1307
Ama87I CYCGRG 1 cut(s) 1027
Aor13HI TCCGGA 1 cut(s) 1046
AoxI GGCC 6 cut(s) 188, 330, 345, 724, 756, 1139
ApeKI GCWGC 4 cut(s) 129, 720, 905, 1109
ApoI RAATTY 4 cut(s) 7, 240, 285, 586
ArsI GACNNNNNNTTYG 2 cut(s) 47, 79
AspLEI GCGC 2 cut(s) 884, 1109
AspS9I GGNCC 3 cut(s) 188, 346, 1139
AsuC2I CCSGG 7 cut(s) 344, 349, 1028, 1029, 1137, 1143, 1404
AsuHPI GGTGA 3 cut(s) 397, 1157, 1201
AvaI CYCGRG 1 cut(s) 1027
BaeGI GKGCMC 1 cut(s) 404
BanII GRGCYC 1 cut(s) 1152
BarI GAAGNNNNNNTAC 2 cut(s) 809, 841
Bbv12I GWGCWC 1 cut(s) 1152
BbvCI CCTCAGC 2 cut(s) 517, 872
BbvI GCAGC 4 cut(s) 141, 707, 892, 1121
BccI CCATC 2 cut(s) 800, 1078
BceAI ACGGC 4 cut(s) 608, 771, 938, 973
BciVI GTATCC 1 cut(s) 1206
BcnI CCSGG 7 cut(s) 344, 349, 1028, 1029, 1137, 1143, 1404
BcoDI GTCTC 3 cut(s) 630, 776, 1381
BfaI CTAG 2 cut(s) 245, 1154
BfmI CTRYAG 1 cut(s) 127
BfoI RGCGCY 1 cut(s) 885
BfuAI ACCTGC 2 cut(s) 134, 1273
BfuI GTATCC 1 cut(s) 1206
BglI GCCNNNNNGGC 1 cut(s) 353
BisI GCNGC 5 cut(s) 130, 721, 724, 906, 1110
BlpI GCTNAGC 1 cut(s) 601
BlsI GCNGC 5 cut(s) 131, 722, 725, 907, 1111
Bme1390I CCNGG 7 cut(s) 344, 349, 1028, 1029, 1137, 1143, 1404
BmeT110I CYCGRG 1 cut(s) 1027
BmgBI CACGTC 1 cut(s) 697
BmgT120I GGNCC 3 cut(s) 188, 346, 1139
BmiI GGNNCC 3 cut(s) 501, 1051, 1140
BmrFI CCNGG 7 cut(s) 344, 349, 1028, 1029, 1137, 1143, 1404
BmsI GCATC 6 cut(s) 73, 170, 573, 587, 984, 1087
BplI GAGNNNNNCTC 2 cut(s) 1374, 1406
Bpu10I CCTNAGC 2 cut(s) 517, 872
Bpu1102I GCTNAGC 1 cut(s) 601
BpuMI CCSGG 7 cut(s) 344, 349, 1028, 1029, 1137, 1143, 1404
BsaAI YACGTR 1 cut(s) 382
BsaI GGTCTC 1 cut(s) 776
BsaJI CCNNGG 7 cut(s) 69, 364, 624, 1026, 1027, 1028, 1136
BsaWI WCCGGW 3 cut(s) 437, 1046, 1250
BsaXI ACNNNNNCTCC 6 cut(s) 670, 700, 773, 803, 1352, 1382
Bsc4I CCNNNNNNNGG 5 cut(s) 328, 476, 496, 1092, 1142
Bse118I RCCGGY 1 cut(s) 758
Bse1I ACTGG 1 cut(s) 868
Bse3DI GCAATG 1 cut(s) 1323
BseAI TCCGGA 1 cut(s) 1046
BseDI CCNNGG 7 cut(s) 69, 364, 624, 1026, 1027, 1028, 1136
BseGI GGATG 5 cut(s) 564, 641, 1022, 1070, 1102
BseLI CCNNNNNNNGG 5 cut(s) 328, 476, 496, 1092, 1142
BseMI GCAATG 1 cut(s) 1323
BseMII CTCAG 4 cut(s) 531, 586, 886, 1358
BseNI ACTGG 1 cut(s) 868
BseSI GKGCMC 1 cut(s) 404
BseX3I CGGCCG 1 cut(s) 756
BseXI GCAGC 4 cut(s) 141, 707, 892, 1121
BseYI CCCAGC 1 cut(s) 1301
Bsh1236I CGCG 2 cut(s) 1063, 1107
Bsh1285I CGRYCG 1 cut(s) 759
BshFI GGCC 6 cut(s) 190, 332, 347, 726, 758, 1141
BsiEI CGRYCG 1 cut(s) 759
BsiHKAI GWGCWC 1 cut(s) 1152
BsiHKCI CYCGRG 1 cut(s) 1027
BslFI GGGAC 1 cut(s) 1045
BslI CCNNNNNNNGG 5 cut(s) 328, 476, 496, 1092, 1142
BsmAI GTCTC 3 cut(s) 630, 776, 1381
BsmFI GGGAC 1 cut(s) 1045
BsnI GGCC 6 cut(s) 190, 332, 347, 726, 758, 1141
Bso31I GGTCTC 1 cut(s) 776
BsoBI CYCGRG 1 cut(s) 1027
Bsp1286I GDGCHC 2 cut(s) 404, 1152
Bsp13I TCCGGA 1 cut(s) 1046
Bsp143I GATC 4 cut(s) 97, 511, 1035, 1312
Bsp1720I GCTNAGC 1 cut(s) 601
Bsp68I TCGCGA 1 cut(s) 1063
BspACI CCGC 6 cut(s) 103, 723, 778, 823, 852, 1086
BspANI GGCC 6 cut(s) 190, 332, 347, 726, 758, 1141
BspCNI CTCAG 4 cut(s) 530, 585, 885, 1357
BspEI TCCGGA 1 cut(s) 1046
BspFNI CGCG 2 cut(s) 1063, 1107
BspLI GGNNCC 3 cut(s) 501, 1051, 1140
BspMAI CTGCAG 1 cut(s) 131
BspMI ACCTGC 2 cut(s) 134, 1273
BspPI GGATC 2 cut(s) 506, 1307
BspTNI GGTCTC 1 cut(s) 776
BsrBI CCGCTC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 1323
BsrFI RCCGGY 1 cut(s) 758
BsrI ACTGG 1 cut(s) 868
BssAI RCCGGY 1 cut(s) 758
BssECI CCNNGG 7 cut(s) 69, 364, 624, 1026, 1027, 1028, 1136
BssMI GATC 4 cut(s) 97, 511, 1035, 1312
BssNAI GTATAC 2 cut(s) 764, 1083
BssT1I CCWWGG 2 cut(s) 69, 364
Bst1107I GTATAC 2 cut(s) 764, 1083
Bst4CI ACNGT 4 cut(s) 94, 391, 839, 1054
BstAPI GCANNNNNTGC 1 cut(s) 561
BstBAI YACGTR 1 cut(s) 382
BstC8I GCNNGC 4 cut(s) 117, 373, 478, 856
BstDEI CTNAG 6 cut(s) 517, 572, 601, 872, 1172, 1344
BstDSI CCRYGG 1 cut(s) 624
BstEII GGTNACC 1 cut(s) 866
BstF5I GGATG 5 cut(s) 564, 641, 1022, 1070, 1102
BstFNI CGCG 2 cut(s) 1063, 1107
BstH2I RGCGCY 1 cut(s) 885
BstHHI GCGC 2 cut(s) 884, 1109
BstKTI GATC 4 cut(s) 100, 514, 1038, 1315
BstMAI GTCTC 3 cut(s) 630, 776, 1381
BstMBI GATC 4 cut(s) 97, 511, 1035, 1312
BstMCI CGRYCG 1 cut(s) 759
BstMWI GCNNNNNNNGC 6 cut(s) 353, 561, 902, 1085, 1106, 1147
BstNSI RCATGY 2 cut(s) 615, 1164
BstPI GGTNACC 1 cut(s) 866
BstSCI CCNGG 7 cut(s) 342, 347, 1026, 1027, 1135, 1141, 1402
BstSFI CTRYAG 1 cut(s) 127
BstSLI GKGCMC 1 cut(s) 404
BstUI CGCG 2 cut(s) 1063, 1107
BstV1I GCAGC 4 cut(s) 141, 707, 892, 1121
BstZ17I GTATAC 2 cut(s) 764, 1083
BstZI CGGCCG 1 cut(s) 756
BsuI GTATCC 1 cut(s) 1206
BsuRI GGCC 6 cut(s) 190, 332, 347, 726, 758, 1141
BtgI CCRYGG 1 cut(s) 624
BtgZI GCGATG 1 cut(s) 1127
BtrI CACGTC 1 cut(s) 697
BtsCI GGATG 5 cut(s) 564, 641, 1022, 1070, 1102
BtsIMutI CAGTG 1 cut(s) 396
BtuMI TCGCGA 1 cut(s) 1063
BveI ACCTGC 2 cut(s) 134, 1273
Cac8I GCNNGC 4 cut(s) 117, 373, 478, 856
CfoI GCGC 2 cut(s) 884, 1109
Cfr10I RCCGGY 1 cut(s) 758
Cfr13I GGNCC 3 cut(s) 188, 346, 1139
Cfr9I CCCGGG 1 cut(s) 1027
CseI GACGC 1 cut(s) 1253
Csp6I GTAC 2 cut(s) 176, 817
CviAII CATG 4 cut(s) 185, 192, 612, 1161
CviQI GTAC 2 cut(s) 176, 817
DdeI CTNAG 6 cut(s) 517, 572, 601, 872, 1172, 1344
DpnI GATC 4 cut(s) 99, 513, 1037, 1314
DpnII GATC 4 cut(s) 97, 511, 1035, 1312
EaeI YGGCCR 2 cut(s) 724, 756
EagI CGGCCG 1 cut(s) 756
Ecl136II GAGCTC 1 cut(s) 1150
EclXI CGGCCG 1 cut(s) 756
Eco130I CCWWGG 2 cut(s) 69, 364
Eco24I GRGCYC 1 cut(s) 1152
Eco31I GGTCTC 1 cut(s) 776
Eco52I CGGCCG 1 cut(s) 756
Eco53kI GAGCTC 1 cut(s) 1150
Eco88I CYCGRG 1 cut(s) 1027
Eco91I GGTNACC 1 cut(s) 866
EcoICRI GAGCTC 1 cut(s) 1150
EcoO65I GGTNACC 1 cut(s) 866
EcoT14I CCWWGG 2 cut(s) 69, 364
EcoT22I ATGCAT 1 cut(s) 1102
EcoT38I GRGCYC 1 cut(s) 1152
ErhI CCWWGG 2 cut(s) 69, 364
FaeI CATG 4 cut(s) 188, 195, 615, 1164
FalI AAGNNNNNCTT 2 cut(s) 665, 697
FaqI GGGAC 1 cut(s) 1045
FatI CATG 4 cut(s) 184, 191, 611, 1160
FauI CCCGC 1 cut(s) 785
FblI GTMKAC 3 cut(s) 763, 1082, 1260
Fnu4HI GCNGC 5 cut(s) 130, 721, 724, 906, 1110
FokI GGATG 5 cut(s) 551, 628, 1009, 1057, 1109
FriOI GRGCYC 1 cut(s) 1152
Fsp4HI GCNGC 5 cut(s) 130, 721, 724, 906, 1110
FspBI CTAG 2 cut(s) 245, 1154
GlaI GCGC 2 cut(s) 883, 1108
GluI GCNGC 5 cut(s) 130, 721, 724, 906, 1110
GsaI CCCAGC 1 cut(s) 1305
HaeII RGCGCY 1 cut(s) 885
HaeIII GGCC 6 cut(s) 190, 332, 347, 726, 758, 1141
HgaI GACGC 1 cut(s) 1253
HhaI GCGC 2 cut(s) 884, 1109
Hin1II CATG 4 cut(s) 188, 195, 615, 1164
Hin6I GCGC 2 cut(s) 882, 1107
HinP1I GCGC 2 cut(s) 882, 1107
HinfI GANTC 6 cut(s) 40, 229, 457, 638, 1043, 1389
HphI GGTGA 3 cut(s) 397, 1157, 1201
Hpy166II GTNNAC 6 cut(s) 178, 268, 764, 985, 1083, 1261
Hpy188I TCNGA 4 cut(s) 328, 949, 1200, 1394
Hpy188III TCNNGA 8 cut(s) 167, 245, 282, 292, 574, 1047, 1062, 1336
Hpy8I GTNNAC 6 cut(s) 178, 268, 764, 985, 1083, 1261
Hpy99I CGWCG 2 cut(s) 698, 1226
HpyAV CCTTC 2 cut(s) 151, 731
HpyCH4III ACNGT 4 cut(s) 94, 391, 839, 1054
HpyCH4IV ACGT 4 cut(s) 381, 696, 1232, 1374
HpyCH4V TGCA 4 cut(s) 129, 371, 564, 1100
HpyF10VI GCNNNNNNNGC 6 cut(s) 353, 561, 902, 1085, 1106, 1147
HpyF3I CTNAG 6 cut(s) 517, 572, 601, 872, 1172, 1344
HpySE526I ACGT 4 cut(s) 381, 696, 1232, 1374
Hsp92II CATG 4 cut(s) 188, 195, 615, 1164
HspAI GCGC 2 cut(s) 882, 1107
Kpn2I TCCGGA 1 cut(s) 1046
Kzo9I GATC 4 cut(s) 97, 511, 1035, 1312
LmnI GCTCC 6 cut(s) 499, 889, 893, 1002, 1155, 1405
Lsp1109I GCAGC 4 cut(s) 141, 707, 892, 1121
LweI GCATC 6 cut(s) 73, 170, 573, 587, 984, 1087
MaeI CTAG 2 cut(s) 245, 1154
MaeII ACGT 4 cut(s) 381, 696, 1232, 1374
MaeIII GTNAC 3 cut(s) 385, 866, 1228
MalI GATC 4 cut(s) 99, 513, 1037, 1314
MbiI CCGCTC 1 cut(s) 105
MboI GATC 4 cut(s) 97, 511, 1035, 1312
MboII GAAGA 3 cut(s) 703, 820, 1363
MhlI GDGCHC 2 cut(s) 404, 1152
MluCI AATT 8 cut(s) 7, 240, 285, 296, 586, 1002, 1238, 1254
MlyI GAGTC 5 cut(s) 34, 451, 632, 1037, 1383
MmeI TCCRAC 1 cut(s) 592
Mph1103I ATGCAT 1 cut(s) 1102
MroI TCCGGA 1 cut(s) 1046
MseI TTAA 1 cut(s) 683
MslI CAYNNNNRTG 3 cut(s) 183, 965, 980
MspR9I CCNGG 7 cut(s) 344, 349, 1028, 1029, 1137, 1143, 1404
MvnI CGCG 2 cut(s) 1063, 1107
MwoI GCNNNNNNNGC 6 cut(s) 353, 561, 902, 1085, 1106, 1147
NciI CCSGG 7 cut(s) 344, 349, 1028, 1029, 1137, 1143, 1404
NdeII GATC 4 cut(s) 97, 511, 1035, 1312
NlaIII CATG 4 cut(s) 188, 195, 615, 1164
NlaIV GGNNCC 3 cut(s) 501, 1051, 1140
NmuCI GTSAC 1 cut(s) 385
NruI TCGCGA 1 cut(s) 1063
NsiI ATGCAT 1 cut(s) 1102
NspI RCATGY 2 cut(s) 615, 1164
PfeI GAWTC 1 cut(s) 229
PkrI GCNGC 5 cut(s) 131, 722, 725, 907, 1111
PleI GAGTC 5 cut(s) 34, 451, 632, 1037, 1383
PpsI GAGTC 5 cut(s) 34, 451, 632, 1037, 1383
Ppu21I YACGTR 1 cut(s) 382
Psp124BI GAGCTC 1 cut(s) 1152
PspEI GGTNACC 1 cut(s) 866
PspFI CCCAGC 1 cut(s) 1301
PspN4I GGNNCC 3 cut(s) 501, 1051, 1140
PspPI GGNCC 3 cut(s) 188, 346, 1139
PsrI GAACNNNNNNTAC 2 cut(s) 792, 824
PstI CTGCAG 1 cut(s) 131
RruI TCGCGA 1 cut(s) 1063
RsaI GTAC 2 cut(s) 177, 818
RsaNI GTAC 2 cut(s) 176, 817
RseI CAYNNNNRTG 3 cut(s) 183, 965, 980
SacI GAGCTC 1 cut(s) 1152
SaqAI TTAA 1 cut(s) 683
SatI GCNGC 5 cut(s) 130, 721, 724, 906, 1110
Sau3AI GATC 4 cut(s) 97, 511, 1035, 1312
Sau96I GGNCC 3 cut(s) 188, 346, 1139
SchI GAGTC 5 cut(s) 34, 451, 632, 1037, 1383
ScrFI CCNGG 7 cut(s) 344, 349, 1028, 1029, 1137, 1143, 1404
SduI GDGCHC 2 cut(s) 404, 1152
SfaNI GCATC 6 cut(s) 73, 170, 573, 587, 984, 1087
SfcI CTRYAG 1 cut(s) 127
SmaI CCCGGG 1 cut(s) 1029
SmiMI CAYNNNNRTG 3 cut(s) 183, 965, 980
Sse9I AATT 8 cut(s) 7, 240, 285, 296, 586, 1002, 1238, 1254
SsiI CCGC 6 cut(s) 103, 723, 778, 823, 852, 1086
SspMI CTAG 2 cut(s) 245, 1154
SstI GAGCTC 1 cut(s) 1152
StyD4I CCNGG 7 cut(s) 342, 347, 1026, 1027, 1135, 1141, 1402
StyI CCWWGG 2 cut(s) 69, 364
TaaI ACNGT 4 cut(s) 94, 391, 839, 1054
TaiI ACGT 4 cut(s) 384, 699, 1235, 1377
TaqI TCGA 2 cut(s) 510, 1041
TasI AATT 8 cut(s) 7, 240, 285, 296, 586, 1002, 1238, 1254
TatI WGTACW 1 cut(s) 816
TauI GCSGC 1 cut(s) 726
TfiI GAWTC 1 cut(s) 229
Tru1I TTAA 1 cut(s) 683
Tru9I TTAA 1 cut(s) 683
TscAI CASTG 1 cut(s) 396
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 4 cut(s) 129, 720, 905, 1109
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 3 cut(s) 242, 733, 1011
TspGWI ACGGA 2 cut(s) 1002, 1116
TspMI CCCGGG 1 cut(s) 1027
TspRI CASTG 1 cut(s) 396
XapI RAATTY 4 cut(s) 7, 240, 285, 586
XbaI TCTAGA 1 cut(s) 244
XceI RCATGY 2 cut(s) 615, 1164
XcmI CCANNNNNNNNNTGG 1 cut(s) 473
XmaI CCCGGG 1 cut(s) 1027
XmiI GTMKAC 3 cut(s) 763, 1082, 1260
XspI CTAG 2 cut(s) 245, 1154
Zsp2I ATGCAT 1 cut(s) 1102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.