Belongs to the peptidase A1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 1300, 1378 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 766 |
| AatII | GACGTC | 2 cut(s) | 1027, 1404 |
| Acc36I | ACCTGC | 3 cut(s) | 175, 1300, 1378 |
| AccBSI | CCGCTC | 1 cut(s) | 1206 |
| AccI | GTMKAC | 2 cut(s) | 360, 605 |
| AccII | CGCG | 4 cut(s) | 41, 482, 846, 1096 |
| AclWI | GGATC | 1 cut(s) | 328 |
| AcoI | YGGCCR | 3 cut(s) | 804, 857, 1296 |
| AcuI | CTGAAG | 2 cut(s) | 993, 1029 |
| AcvI | CACGTG | 1 cut(s) | 1191 |
| AcyI | GRCGYC | 2 cut(s) | 1024, 1401 |
| AfaI | GTAC | 5 cut(s) | 50, 121, 261, 855, 1115 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 343, 1362 |
| AflIII | ACRYGT | 1 cut(s) | 1188 |
| AgsI | TTSAA | 2 cut(s) | 139, 1373 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 534 |
| AjnI | CCWGG | 3 cut(s) | 223, 667, 993 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 452, 484, 1223, 1255 |
| AluBI | AGCT | 5 cut(s) | 146, 912, 1155, 1166, 1174 |
| AluI | AGCT | 5 cut(s) | 146, 912, 1155, 1166, 1174 |
| AlwI | GGATC | 1 cut(s) | 328 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1085 |
| Ama87I | CYCGRG | 1 cut(s) | 442 |
| AoxI | GGCC | 6 cut(s) | 539, 804, 819, 857, 991, 1296 |
| ApeKI | GCWGC | 4 cut(s) | 27, 170, 429, 1212 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 92, 124, 1252, 1284 |
| AspLEI | GCGC | 3 cut(s) | 846, 848, 896 |
| AspS9I | GGNCC | 4 cut(s) | 272, 346, 929, 981 |
| AsuC2I | CCSGG | 2 cut(s) | 376, 511 |
| AsuHPI | GGTGA | 3 cut(s) | 213, 395, 468 |
| AvaI | CYCGRG | 1 cut(s) | 442 |
| AvaII | GGWCC | 4 cut(s) | 272, 346, 929, 981 |
| AxyI | CCTNAGG | 2 cut(s) | 392, 1060 |
| BalI | TGGCCA | 2 cut(s) | 806, 1298 |
| BanII | GRGCYC | 3 cut(s) | 443, 476, 576 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 846, 878 |
| BbrPI | CACGTG | 1 cut(s) | 1191 |
| BbvCI | CCTCAGC | 2 cut(s) | 908, 1167 |
| BbvI | GCAGC | 4 cut(s) | 14, 182, 441, 1224 |
| BccI | CCATC | 3 cut(s) | 419, 584, 595 |
| BceAI | ACGGC | 3 cut(s) | 872, 926, 1006 |
| BciT130I | CCWGG | 3 cut(s) | 225, 669, 995 |
| BciVI | GTATCC | 2 cut(s) | 731, 1236 |
| BcnI | CCSGG | 2 cut(s) | 376, 511 |
| BfmI | CTRYAG | 2 cut(s) | 168, 836 |
| BfoI | RGCGCY | 1 cut(s) | 897 |
| BfuAI | ACCTGC | 3 cut(s) | 175, 1300, 1378 |
| BfuI | GTATCC | 2 cut(s) | 731, 1236 |
| BisI | GCNGC | 8 cut(s) | 28, 171, 430, 682, 860, 956, 1097, 1213 |
| BlsI | GCNGC | 8 cut(s) | 29, 172, 431, 683, 861, 957, 1098, 1214 |
| BmcAI | AGTACT | 2 cut(s) | 50, 121 |
| Bme1390I | CCNGG | 5 cut(s) | 225, 376, 511, 669, 995 |
| Bme18I | GGWCC | 4 cut(s) | 272, 346, 929, 981 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 534 |
| BmeT110I | CYCGRG | 1 cut(s) | 442 |
| BmgT120I | GGNCC | 4 cut(s) | 272, 346, 929, 981 |
| BmiI | GGNNCC | 2 cut(s) | 573, 982 |
| BmrFI | CCNGG | 5 cut(s) | 225, 376, 511, 669, 995 |
| BmrI | ACTGGG | 1 cut(s) | 6 |
| BmsI | GCATC | 4 cut(s) | 169, 254, 1120, 1264 |
| BmuI | ACTGGG | 1 cut(s) | 6 |
| BpmI | CTGGAG | 1 cut(s) | 323 |
| Bpu10I | CCTNAGC | 3 cut(s) | 908, 1167, 1202 |
| BpuEI | CTTGAG | 1 cut(s) | 661 |
| BpuMI | CCSGG | 2 cut(s) | 376, 511 |
| Bsa29I | ATCGAT | 1 cut(s) | 1034 |
| BsaAI | YACGTR | 2 cut(s) | 1191, 1294 |
| BsaHI | GRCGYC | 2 cut(s) | 1024, 1401 |
| BsaJI | CCNNGG | 1 cut(s) | 732 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 301, 331, 713, 743 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 343, 1362 |
| Bse1I | ACTGG | 5 cut(s) | 12, 629, 904, 1188, 1299 |
| Bse21I | CCTNAGG | 2 cut(s) | 392, 1060 |
| Bse3DI | GCAATG | 1 cut(s) | 1350 |
| BseBI | CCWGG | 3 cut(s) | 225, 669, 995 |
| BseCI | ATCGAT | 1 cut(s) | 1034 |
| BseDI | CCNNGG | 1 cut(s) | 732 |
| BseGI | GGATG | 6 cut(s) | 407, 411, 587, 1003, 1135, 1279 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 343, 1362 |
| BseMI | GCAATG | 1 cut(s) | 1350 |
| BseMII | CTCAG | 7 cut(s) | 344, 545, 922, 1074, 1092, 1158, 1193 |
| BseNI | ACTGG | 5 cut(s) | 12, 629, 904, 1188, 1299 |
| BsePI | GCGCGC | 1 cut(s) | 844 |
| BseRI | GAGGAG | 1 cut(s) | 1184 |
| BseX3I | CGGCCG | 1 cut(s) | 857 |
| BseXI | GCAGC | 4 cut(s) | 14, 182, 441, 1224 |
| BseYI | CCCAGC | 1 cut(s) | 1328 |
| Bsh1236I | CGCG | 4 cut(s) | 41, 482, 846, 1096 |
| Bsh1285I | CGRYCG | 1 cut(s) | 860 |
| BshFI | GGCC | 6 cut(s) | 541, 806, 821, 859, 993, 1298 |
| BshVI | ATCGAT | 1 cut(s) | 1034 |
| BsiEI | CGRYCG | 1 cut(s) | 860 |
| BsiHKCI | CYCGRG | 1 cut(s) | 442 |
| BsiSI | CCGG | 4 cut(s) | 376, 426, 510, 1103 |
| BslFI | GGGAC | 2 cut(s) | 1078, 1096 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 343, 1362 |
| BsmFI | GGGAC | 2 cut(s) | 1078, 1096 |
| BsnI | GGCC | 6 cut(s) | 541, 806, 821, 859, 993, 1298 |
| BsoBI | CYCGRG | 1 cut(s) | 442 |
| Bsp1286I | GDGCHC | 3 cut(s) | 443, 476, 576 |
| Bsp143I | GATC | 2 cut(s) | 333, 1068 |
| Bsp68I | TCGCGA | 1 cut(s) | 41 |
| BspANI | GGCC | 6 cut(s) | 541, 806, 821, 859, 993, 1298 |
| BspCNI | CTCAG | 7 cut(s) | 343, 544, 921, 1073, 1091, 1159, 1194 |
| BspDI | ATCGAT | 1 cut(s) | 1034 |
| BspFNI | CGCG | 4 cut(s) | 41, 482, 846, 1096 |
| BspHI | TCATGA | 1 cut(s) | 229 |
| BspLI | GGNNCC | 2 cut(s) | 573, 982 |
| BspMAI | CTGCAG | 2 cut(s) | 172, 840 |
| BspMI | ACCTGC | 3 cut(s) | 175, 1300, 1378 |
| BspPI | GGATC | 1 cut(s) | 328 |
| BsrBI | CCGCTC | 1 cut(s) | 1206 |
| BsrDI | GCAATG | 1 cut(s) | 1350 |
| BsrI | ACTGG | 5 cut(s) | 12, 629, 904, 1188, 1299 |
| BssECI | CCNNGG | 1 cut(s) | 732 |
| BssHII | GCGCGC | 1 cut(s) | 844 |
| BssMI | GATC | 2 cut(s) | 333, 1068 |
| BssNAI | GTATAC | 1 cut(s) | 606 |
| BssNI | GRCGYC | 2 cut(s) | 1024, 1401 |
| Bst1107I | GTATAC | 1 cut(s) | 606 |
| Bst2UI | CCWGG | 3 cut(s) | 225, 669, 995 |
| Bst4CI | ACNGT | 8 cut(s) | 788, 967, 1087, 1118, 1219, 1337, 1346, 1383 |
| Bst6I | CTCTTC | 3 cut(s) | 97, 799, 1048 |
| BstACI | GRCGYC | 2 cut(s) | 1024, 1401 |
| BstBAI | YACGTR | 2 cut(s) | 1191, 1294 |
| BstC8I | GCNNGC | 1 cut(s) | 846 |
| BstDSI | CCRYGG | 1 cut(s) | 732 |
| BstEII | GGTNACC | 1 cut(s) | 902 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 341 |
| BstF5I | GGATG | 6 cut(s) | 407, 411, 587, 1003, 1135, 1279 |
| BstFNI | CGCG | 4 cut(s) | 41, 482, 846, 1096 |
| BstH2I | RGCGCY | 1 cut(s) | 897 |
| BstHHI | GCGC | 3 cut(s) | 846, 848, 896 |
| BstKTI | GATC | 2 cut(s) | 336, 1071 |
| BstMBI | GATC | 2 cut(s) | 333, 1068 |
| BstMCI | CGRYCG | 1 cut(s) | 860 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 438, 681, 702, 1118, 1212 |
| BstNI | CCWGG | 3 cut(s) | 225, 669, 995 |
| BstNSI | RCATGY | 1 cut(s) | 268 |
| BstPI | GGTNACC | 1 cut(s) | 902 |
| BstSCI | CCNGG | 5 cut(s) | 223, 374, 509, 667, 993 |
| BstSFI | CTRYAG | 2 cut(s) | 168, 836 |
| BstUI | CGCG | 4 cut(s) | 41, 482, 846, 1096 |
| BstV1I | GCAGC | 4 cut(s) | 14, 182, 441, 1224 |
| BstX2I | RGATCY | 1 cut(s) | 333 |
| BstYI | RGATCY | 1 cut(s) | 333 |
| BstZ17I | GTATAC | 1 cut(s) | 606 |
| BstZI | CGGCCG | 1 cut(s) | 857 |
| Bsu15I | ATCGAT | 1 cut(s) | 1034 |
| Bsu36I | CCTNAGG | 2 cut(s) | 392, 1060 |
| BsuI | GTATCC | 2 cut(s) | 731, 1236 |
| BsuRI | GGCC | 6 cut(s) | 541, 806, 821, 859, 993, 1298 |
| BsuTUI | ATCGAT | 1 cut(s) | 1034 |
| BtgI | CCRYGG | 1 cut(s) | 732 |
| BtsCI | GGATG | 6 cut(s) | 407, 411, 587, 1003, 1135, 1279 |
| BtsIMutI | CAGTG | 9 cut(s) | 19, 539, 897, 963, 1123, 1224, 1306, 1342, 1351 |
| BtuMI | TCGCGA | 1 cut(s) | 41 |
| BveI | ACCTGC | 3 cut(s) | 175, 1300, 1378 |
| Cac8I | GCNNGC | 1 cut(s) | 846 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1085 |
| CciI | TCATGA | 1 cut(s) | 229 |
| CfoI | GCGC | 3 cut(s) | 846, 848, 896 |
| Cfr13I | GGNCC | 4 cut(s) | 272, 346, 929, 981 |
| ClaI | ATCGAT | 1 cut(s) | 1034 |
| CsiI | ACCWGGT | 2 cut(s) | 223, 667 |
| Csp6I | GTAC | 5 cut(s) | 49, 120, 260, 854, 1114 |
| CviAII | CATG | 4 cut(s) | 230, 265, 269, 276 |
| CviQI | GTAC | 5 cut(s) | 49, 120, 260, 854, 1114 |
| DpnI | GATC | 2 cut(s) | 335, 1070 |
| DpnII | GATC | 2 cut(s) | 333, 1068 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 766 |
| DriI | GACNNNNNGTC | 1 cut(s) | 534 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 766 |
| EaeI | YGGCCR | 3 cut(s) | 804, 857, 1296 |
| EagI | CGGCCG | 1 cut(s) | 857 |
| Eam1104I | CTCTTC | 3 cut(s) | 97, 799, 1048 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 534 |
| EarI | CTCTTC | 3 cut(s) | 97, 799, 1048 |
| EciI | GGCGGA | 1 cut(s) | 1035 |
| EclXI | CGGCCG | 1 cut(s) | 857 |
| Eco147I | AGGCCT | 1 cut(s) | 821 |
| Eco24I | GRGCYC | 3 cut(s) | 443, 476, 576 |
| Eco47I | GGWCC | 4 cut(s) | 272, 346, 929, 981 |
| Eco52I | CGGCCG | 1 cut(s) | 857 |
| Eco57I | CTGAAG | 2 cut(s) | 993, 1029 |
| Eco72I | CACGTG | 1 cut(s) | 1191 |
| Eco81I | CCTNAGG | 2 cut(s) | 392, 1060 |
| Eco88I | CYCGRG | 1 cut(s) | 442 |
| Eco91I | GGTNACC | 1 cut(s) | 902 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 341 |
| EcoO109I | RGGNCCY | 1 cut(s) | 981 |
| EcoO65I | GGTNACC | 1 cut(s) | 902 |
| EcoRII | CCWGG | 3 cut(s) | 223, 667, 993 |
| EcoT22I | ATGCAT | 1 cut(s) | 270 |
| EcoT38I | GRGCYC | 3 cut(s) | 443, 476, 576 |
| FaeI | CATG | 4 cut(s) | 233, 268, 272, 279 |
| FaqI | GGGAC | 2 cut(s) | 1078, 1096 |
| FatI | CATG | 4 cut(s) | 229, 264, 268, 275 |
| FauI | CCCGC | 4 cut(s) | 165, 424, 487, 1199 |
| FblI | GTMKAC | 2 cut(s) | 360, 605 |
| Fnu4HI | GCNGC | 8 cut(s) | 28, 171, 430, 682, 860, 956, 1097, 1213 |
| FokI | GGATG | 6 cut(s) | 394, 398, 574, 1010, 1142, 1286 |
| FriOI | GRGCYC | 3 cut(s) | 443, 476, 576 |
| Fsp4HI | GCNGC | 8 cut(s) | 28, 171, 430, 682, 860, 956, 1097, 1213 |
| GlaI | GCGC | 3 cut(s) | 845, 847, 895 |
| GluI | GCNGC | 8 cut(s) | 28, 171, 430, 682, 860, 956, 1097, 1213 |
| GsaI | CCCAGC | 1 cut(s) | 1332 |
| GsuI | CTGGAG | 1 cut(s) | 323 |
| HaeII | RGCGCY | 1 cut(s) | 897 |
| HaeIII | GGCC | 6 cut(s) | 541, 806, 821, 859, 993, 1298 |
| HapII | CCGG | 4 cut(s) | 376, 426, 510, 1103 |
| HhaI | GCGC | 3 cut(s) | 846, 848, 896 |
| Hin1I | GRCGYC | 2 cut(s) | 1024, 1401 |
| Hin1II | CATG | 4 cut(s) | 233, 268, 272, 279 |
| Hin6I | GCGC | 3 cut(s) | 844, 846, 894 |
| HinP1I | GCGC | 3 cut(s) | 844, 846, 894 |
| HincII | GTYRAC | 4 cut(s) | 361, 759, 771, 1260 |
| HindII | GTYRAC | 4 cut(s) | 361, 759, 771, 1260 |
| HinfI | GANTC | 8 cut(s) | 357, 362, 529, 1008, 1036, 1076, 1261, 1448 |
| HpaII | CCGG | 4 cut(s) | 376, 426, 510, 1103 |
| HphI | GGTGA | 3 cut(s) | 213, 395, 468 |
| Hpy166II | GTNNAC | 9 cut(s) | 262, 361, 460, 606, 759, 771, 929, 951, 1260 |
| Hpy188I | TCNGA | 3 cut(s) | 333, 917, 973 |
| Hpy188III | TCNNGA | 5 cut(s) | 40, 98, 230, 444, 1265 |
| Hpy8I | GTNNAC | 9 cut(s) | 262, 361, 460, 606, 759, 771, 929, 951, 1260 |
| Hpy99I | CGWCG | 3 cut(s) | 788, 1029, 1406 |
| HpyAV | CCTTC | 7 cut(s) | 43, 235, 373, 562, 736, 942, 968 |
| HpyCH4III | ACNGT | 8 cut(s) | 788, 967, 1087, 1118, 1219, 1337, 1346, 1383 |
| HpyCH4IV | ACGT | 6 cut(s) | 462, 767, 1024, 1190, 1293, 1401 |
| HpyCH4V | TGCA | 7 cut(s) | 170, 189, 268, 696, 838, 1277, 1387 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 438, 681, 702, 1118, 1212 |
| HpySE526I | ACGT | 6 cut(s) | 462, 767, 1024, 1190, 1293, 1401 |
| Hsp92I | GRCGYC | 2 cut(s) | 1024, 1401 |
| Hsp92II | CATG | 4 cut(s) | 233, 268, 272, 279 |
| HspAI | GCGC | 3 cut(s) | 844, 846, 894 |
| Kzo9I | GATC | 2 cut(s) | 333, 1068 |
| LmnI | GCTCC | 6 cut(s) | 35, 513, 571, 841, 1171, 1209 |
| Lsp1109I | GCAGC | 4 cut(s) | 14, 182, 441, 1224 |
| LweI | GCATC | 4 cut(s) | 169, 254, 1120, 1264 |
| MabI | ACCWGGT | 2 cut(s) | 223, 667 |
| MaeII | ACGT | 6 cut(s) | 462, 767, 1024, 1190, 1293, 1401 |
| MaeIII | GTNAC | 4 cut(s) | 383, 557, 902, 1346 |
| MalI | GATC | 2 cut(s) | 335, 1070 |
| MbiI | CCGCTC | 1 cut(s) | 1206 |
| MboI | GATC | 2 cut(s) | 333, 1068 |
| MboII | GAAGA | 5 cut(s) | 84, 250, 786, 857, 1035 |
| MflI | RGATCY | 1 cut(s) | 333 |
| MhlI | GDGCHC | 3 cut(s) | 443, 476, 576 |
| MlsI | TGGCCA | 2 cut(s) | 806, 1298 |
| MluCI | AATT | 1 cut(s) | 622 |
| MluNI | TGGCCA | 2 cut(s) | 806, 1298 |
| MlyI | GAGTC | 5 cut(s) | 356, 366, 523, 1070, 1255 |
| MmeI | TCCRAC | 4 cut(s) | 344, 960, 985, 1399 |
| Mox20I | TGGCCA | 2 cut(s) | 806, 1298 |
| Mph1103I | ATGCAT | 1 cut(s) | 270 |
| MscI | TGGCCA | 2 cut(s) | 806, 1298 |
| MseI | TTAA | 3 cut(s) | 81, 252, 1137 |
| MslI | CAYNNNNRTG | 1 cut(s) | 267 |
| Msp20I | TGGCCA | 2 cut(s) | 806, 1298 |
| MspA1I | CMGCKG | 1 cut(s) | 1251 |
| MspI | CCGG | 4 cut(s) | 376, 426, 510, 1103 |
| MspR9I | CCNGG | 5 cut(s) | 225, 376, 511, 669, 995 |
| MvaI | CCWGG | 3 cut(s) | 225, 669, 995 |
| MvnI | CGCG | 4 cut(s) | 41, 482, 846, 1096 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 438, 681, 702, 1118, 1212 |
| NciI | CCSGG | 2 cut(s) | 376, 511 |
| NdeII | GATC | 2 cut(s) | 333, 1068 |
| NlaIII | CATG | 4 cut(s) | 233, 268, 272, 279 |
| NlaIV | GGNNCC | 2 cut(s) | 573, 982 |
| NmuCI | GTSAC | 2 cut(s) | 383, 1346 |
| NruI | TCGCGA | 1 cut(s) | 41 |
| NsiI | ATGCAT | 1 cut(s) | 270 |
| NspI | RCATGY | 1 cut(s) | 268 |
| PagI | TCATGA | 1 cut(s) | 229 |
| PaqCI | CACCTGC | 2 cut(s) | 1300, 1378 |
| PauI | GCGCGC | 1 cut(s) | 844 |
| PceI | AGGCCT | 1 cut(s) | 821 |
| PfeI | GAWTC | 3 cut(s) | 1008, 1036, 1448 |
| PkrI | GCNGC | 8 cut(s) | 29, 172, 431, 683, 861, 957, 1098, 1214 |
| PleI | GAGTC | 5 cut(s) | 356, 365, 523, 1070, 1255 |
| PmaCI | CACGTG | 1 cut(s) | 1191 |
| PmlI | CACGTG | 1 cut(s) | 1191 |
| PpsI | GAGTC | 5 cut(s) | 356, 365, 523, 1070, 1255 |
| Ppu21I | YACGTR | 2 cut(s) | 1191, 1294 |
| PpuMI | RGGWCCY | 1 cut(s) | 981 |
| Psp5II | RGGWCCY | 1 cut(s) | 981 |
| Psp6I | CCWGG | 3 cut(s) | 223, 667, 993 |
| PspCI | CACGTG | 1 cut(s) | 1191 |
| PspEI | GGTNACC | 1 cut(s) | 902 |
| PspFI | CCCAGC | 1 cut(s) | 1328 |
| PspGI | CCWGG | 3 cut(s) | 223, 667, 993 |
| PspN4I | GGNNCC | 2 cut(s) | 573, 982 |
| PspPI | GGNCC | 4 cut(s) | 272, 346, 929, 981 |
| PspPPI | RGGWCCY | 1 cut(s) | 981 |
| PstI | CTGCAG | 2 cut(s) | 172, 840 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1085 |
| PsuI | RGATCY | 1 cut(s) | 333 |
| PteI | GCGCGC | 1 cut(s) | 844 |
| RruI | TCGCGA | 1 cut(s) | 41 |
| RsaI | GTAC | 5 cut(s) | 50, 121, 261, 855, 1115 |
| RsaNI | GTAC | 5 cut(s) | 49, 120, 260, 854, 1114 |
| RseI | CAYNNNNRTG | 1 cut(s) | 267 |
| SalI | GTCGAC | 1 cut(s) | 359 |
| SaqAI | TTAA | 3 cut(s) | 81, 252, 1137 |
| SatI | GCNGC | 8 cut(s) | 28, 171, 430, 682, 860, 956, 1097, 1213 |
| Sau3AI | GATC | 2 cut(s) | 333, 1068 |
| Sau96I | GGNCC | 4 cut(s) | 272, 346, 929, 981 |
| ScaI | AGTACT | 2 cut(s) | 50, 121 |
| SchI | GAGTC | 5 cut(s) | 356, 366, 523, 1070, 1255 |
| ScrFI | CCNGG | 5 cut(s) | 225, 376, 511, 669, 995 |
| SduI | GDGCHC | 3 cut(s) | 443, 476, 576 |
| SexAI | ACCWGGT | 2 cut(s) | 223, 667 |
| SfaNI | GCATC | 4 cut(s) | 169, 254, 1120, 1264 |
| SfcI | CTRYAG | 2 cut(s) | 168, 836 |
| SinI | GGWCC | 4 cut(s) | 272, 346, 929, 981 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 267 |
| SmlI | CTYRAG | 1 cut(s) | 676 |
| SmoI | CTYRAG | 1 cut(s) | 676 |
| Sse9I | AATT | 1 cut(s) | 622 |
| SseBI | AGGCCT | 1 cut(s) | 821 |
| SspI | AATATT | 1 cut(s) | 85 |
| StuI | AGGCCT | 1 cut(s) | 821 |
| StyD4I | CCNGG | 5 cut(s) | 223, 374, 509, 667, 993 |
| TaaI | ACNGT | 8 cut(s) | 788, 967, 1087, 1118, 1219, 1337, 1346, 1383 |
| TaiI | ACGT | 6 cut(s) | 465, 770, 1027, 1193, 1296, 1404 |
| TaqI | TCGA | 7 cut(s) | 163, 360, 504, 783, 1034, 1074, 1178 |
| TasI | AATT | 1 cut(s) | 622 |
| TatI | WGTACW | 2 cut(s) | 48, 119 |
| TauI | GCSGC | 4 cut(s) | 684, 862, 958, 1099 |
| TfiI | GAWTC | 3 cut(s) | 1008, 1036, 1448 |
| Tru1I | TTAA | 3 cut(s) | 81, 252, 1137 |
| Tru9I | TTAA | 3 cut(s) | 81, 252, 1137 |
| TscAI | CASTG | 9 cut(s) | 19, 539, 904, 970, 1123, 1224, 1306, 1342, 1351 |
| TseFI | GTSAC | 2 cut(s) | 383, 1346 |
| TseI | GCWGC | 4 cut(s) | 27, 170, 429, 1212 |
| Tsp45I | GTSAC | 2 cut(s) | 383, 1346 |
| TspDTI | ATGAA | 3 cut(s) | 8, 210, 1163 |
| TspGWI | ACGGA | 2 cut(s) | 721, 729 |
| TspRI | CASTG | 9 cut(s) | 19, 539, 904, 970, 1123, 1224, 1306, 1342, 1351 |
| VpaK11BI | GGWCC | 4 cut(s) | 272, 346, 929, 981 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 341 |
| XceI | RCATGY | 1 cut(s) | 268 |
| XmiI | GTMKAC | 2 cut(s) | 360, 605 |
| ZraI | GACGTC | 2 cut(s) | 1025, 1402 |
| ZrmI | AGTACT | 2 cut(s) | 50, 121 |
| Zsp2I | ATGCAT | 1 cut(s) | 270 |