Rw4G009090

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000845
Physical Location & Seq
Forward (+)
20350604 .. 20353718
3115 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G009090.1

Sequence Viewer

Length: 1464 bp
ATGGCAGGTGTTAGTCCTGGCTCCTCCTCCTTCTCCTCTCCCCTCAGTATGAGGAATTTCATCGGTTTGTCTCTACTTCTATGCCTAACTCTCTGCTCTTTGGACAAAGGCTTTGCCTTGGCAGAAGCAGATACCCACACAGTTGATCTCCGTTCTCTATTGCCAGCAACGACCTGTAGCCCCTCCACCAAAGGTAATAAGAGGAAGGCATCTCTTGAAGTGGTACACAAGCATGGGCCATGCTCTCATCTCGCTCAACACAAAGCAACTGCTACGAATCACACTCAAATTCTAGAGCAAGACCAAGTGCGAGTGAACTCAATCAACTCCCGAATTTTGAAGAAATTGAACGGCAACGAGGTTCTAAGCCAATCGAAGGCCACCACCCTCCCGGCCCGATGGGCTACCAGCCAAGGTGCAAGCAACTACGTGGTGACGGTAGGATTGGGCACACCTAACAAACCACTTTCGCTTGTATTTGACACCGGAAGCTATCTCACTTGGACTCAATGCCAACCTTGTGCTGGCTCTTGCTACCCACAAAAGGAGCCCATTTTCGACCCCTCAGCCTCCACCTCATATACCAACATTTCCTGTAATAGCACAGACTGCATCCAACTCAGGACAAGTACAAAATTCAACAAATGCTTAGCAGACACATGCTTATACGCCTTGGTGTATGGAGACTCATCCTACACCATTGGATTATTCGCCACAGACAAGCTGACCTTAACTCCAAACGACGTGTTTGATGGGTTCTTCTTTGGCTGCGGCCAACAAAACGAAGGCTTCATCAATGGTACGGCCGGTATACTTGGTCTCTCCCGCAACAAGTTCTCCATCATTGAACAAACCATACAAAAGTACAACCGCTTCTTCTCCTACTGTCTGCCACCAACCGCAAGCTCTATTGGATACCTCAGCTTCGGCGCACCTGATGGAGCTTCCAATACTGCTGTAAAATACACACGGCTCACTACTTTATCCTTGAGCTCAGATTTTTACGGCATAGACGTGGCTGGTATCAATGTGAACGGAAAAAAGCTCTCAATTCCTGCTTCAGTGTTTTCATCCCCGGGGACGATCATCGACTCTGGGACCGTCATCTCGCGGCTGCCGTCCACAGCGTATACCGCTTTGCGGGATGCATTTCGGGCAGCGATGACAAGCTATCCGTTAGTTCCGGGACCGGGTGAGCTCCTAGATACATGCTATGACCTTAGTAGCTATCCAACGGTGACGTATCCGAAAATAGAGTTTGTGTTCGGCGACGGGGTTACGTTGGAATTGGACGCAACCGGAATTGTCTACTTTCTCAACGGTTTGAAGCAGGTTTGCTTGGCTTTTGCCGGGAATGATGATGCTAATGACATTGCGATTTTCGGGAATACTCAGCAGAAGAATATGGAGGTGCTATATGATGTTGCTGGAGGGAGAGTCGGATTTGCTCCCGGTGGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

487

Amino Acids

52.0

Weight (kDa)

6.95

Isoelectric Point (pI)

33.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 142 - 484 2.5e-23 Eukaryotic aspartyl protease
TAXi_N PF14543 143 - 310 1.8e-49 Xylanase inhibitor N-terminal
TAXi_C PF14541 335 - 483 4.8e-28 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1321
AccI GTMKAC 3 cut(s) 811, 1130, 1308
AccII CGCG 1 cut(s) 1111
AciI CCGC 7 cut(s) 771, 826, 871, 900, 1111, 1134, 1141
AcoI YGGCCR 2 cut(s) 772, 804
AcsI RAATTY 4 cut(s) 55, 288, 333, 635
AcuI CTGAAG 1 cut(s) 1044
AfaI GTAC 4 cut(s) 225, 631, 802, 866
AfiI CCNNNNNNNGG 5 cut(s) 376, 524, 544, 1140, 1190
AflIII ACRYGT 1 cut(s) 744
AgsI TTSAA 6 cut(s) 218, 340, 349, 640, 848, 1327
AjiI CACGTC 2 cut(s) 745, 1015
AjnI CCWGG 1 cut(s) 16
Alw21I GWGCWC 2 cut(s) 995, 1200
Alw26I GTCTC 3 cut(s) 75, 678, 824
Ama87I CYCGRG 1 cut(s) 1075
AoxI GGCC 5 cut(s) 236, 378, 393, 772, 804
ApeKI GCWGC 3 cut(s) 768, 1114, 1157
ApoI RAATTY 4 cut(s) 55, 288, 333, 635
ArsI GACNNNNNNTTYG 2 cut(s) 95, 127
AspLEI GCGC 1 cut(s) 932
AspS9I GGNCC 4 cut(s) 236, 394, 1098, 1187
AsuC2I CCSGG 7 cut(s) 392, 1076, 1077, 1185, 1191, 1351, 1452
AsuHPI GGTGA 3 cut(s) 445, 1205, 1249
AvaI CYCGRG 1 cut(s) 1075
AvaII GGWCC 2 cut(s) 1098, 1187
BaeGI GKGCMC 1 cut(s) 452
BanII GRGCYC 3 cut(s) 552, 995, 1200
BarI GAAGNNNNNNTAC 2 cut(s) 857, 889
Bbv12I GWGCWC 2 cut(s) 995, 1200
BbvCI CCTCAGC 2 cut(s) 565, 920
BbvI GCAGC 3 cut(s) 755, 1101, 1169
BccI CCATC 4 cut(s) 393, 746, 848, 932
BceAI ACGGC 5 cut(s) 367, 819, 986, 1021, 1102
BciT130I CCWGG 1 cut(s) 18
BciVI GTATCC 2 cut(s) 908, 1254
BcnI CCSGG 7 cut(s) 392, 1076, 1077, 1185, 1191, 1351, 1452
BcoDI GTCTC 3 cut(s) 75, 678, 824
BfaI CTAG 2 cut(s) 293, 1202
BfmI CTRYAG 1 cut(s) 175
BfuAI ACCTGC 1 cut(s) 1321
BfuI GTATCC 2 cut(s) 908, 1254
BglI GCCNNNNNGGC 1 cut(s) 401
BisI GCNGC 5 cut(s) 769, 772, 1112, 1115, 1158
BlpI GCTNAGC 1 cut(s) 649
BlsI GCNGC 5 cut(s) 770, 773, 1113, 1116, 1159
Bme1390I CCNGG 8 cut(s) 18, 392, 1076, 1077, 1185, 1191, 1351, 1452
Bme18I GGWCC 2 cut(s) 1098, 1187
BmeT110I CYCGRG 1 cut(s) 1075
BmgBI CACGTC 2 cut(s) 745, 1015
BmgT120I GGNCC 4 cut(s) 236, 394, 1098, 1187
BmiI GGNNCC 4 cut(s) 22, 549, 1099, 1188
BmrFI CCNGG 8 cut(s) 18, 392, 1076, 1077, 1185, 1191, 1351, 1452
BmsI GCATC 4 cut(s) 218, 621, 1135, 1351
BpmI CTGGAG 1 cut(s) 1449
Bpu10I CCTNAGC 2 cut(s) 565, 920
Bpu1102I GCTNAGC 1 cut(s) 649
BpuEI CTTGAG 1 cut(s) 1009
BpuMI CCSGG 7 cut(s) 392, 1076, 1077, 1185, 1191, 1351, 1452
BsaAI YACGTR 1 cut(s) 430
BsaI GGTCTC 1 cut(s) 824
BsaJI CCNNGG 6 cut(s) 117, 412, 672, 1074, 1075, 1076
BsaWI WCCGGW 2 cut(s) 485, 1298
BsaXI ACNNNNNCTCC 6 cut(s) 718, 748, 821, 851, 1422, 1452
Bsc4I CCNNNNNNNGG 5 cut(s) 376, 524, 544, 1140, 1190
Bse118I RCCGGY 1 cut(s) 806
Bse3DI GCAATG 1 cut(s) 1371
BseBI CCWGG 1 cut(s) 18
BseDI CCNNGG 6 cut(s) 117, 412, 672, 1074, 1075, 1076
BseGI GGATG 4 cut(s) 612, 689, 1070, 1150
BseLI CCNNNNNNNGG 5 cut(s) 376, 524, 544, 1140, 1190
BseMI GCAATG 1 cut(s) 1371
BseMII CTCAG 6 cut(s) 58, 579, 634, 934, 1008, 1406
BseRI GAGGAG 3 cut(s) 13, 16, 25
BseSI GKGCMC 1 cut(s) 452
BseX3I CGGCCG 1 cut(s) 804
BseXI GCAGC 3 cut(s) 755, 1101, 1169
Bsh1236I CGCG 1 cut(s) 1111
Bsh1285I CGRYCG 1 cut(s) 807
BshFI GGCC 5 cut(s) 238, 380, 395, 774, 806
BsiEI CGRYCG 1 cut(s) 807
BsiHKAI GWGCWC 2 cut(s) 995, 1200
BsiHKCI CYCGRG 1 cut(s) 1075
BsiSI CCGG 9 cut(s) 392, 486, 807, 1076, 1184, 1190, 1299, 1350, 1452
BslFI GGGAC 3 cut(s) 1093, 1111, 1200
BslI CCNNNNNNNGG 5 cut(s) 376, 524, 544, 1140, 1190
BsmAI GTCTC 3 cut(s) 75, 678, 824
BsmFI GGGAC 3 cut(s) 1093, 1111, 1200
BsnI GGCC 5 cut(s) 238, 380, 395, 774, 806
Bso31I GGTCTC 1 cut(s) 824
BsoBI CYCGRG 1 cut(s) 1075
Bsp1286I GDGCHC 4 cut(s) 452, 552, 995, 1200
Bsp143I GATC 2 cut(s) 145, 1083
Bsp1720I GCTNAGC 1 cut(s) 649
BspACI CCGC 7 cut(s) 771, 826, 871, 900, 1111, 1134, 1141
BspANI GGCC 5 cut(s) 238, 380, 395, 774, 806
BspCNI CTCAG 6 cut(s) 57, 578, 633, 933, 1007, 1405
BspFNI CGCG 1 cut(s) 1111
BspLI GGNNCC 4 cut(s) 22, 549, 1099, 1188
BspMI ACCTGC 1 cut(s) 1321
BspTNI GGTCTC 1 cut(s) 824
BsrDI GCAATG 1 cut(s) 1371
BsrFI RCCGGY 1 cut(s) 806
BssAI RCCGGY 1 cut(s) 806
BssECI CCNNGG 6 cut(s) 117, 412, 672, 1074, 1075, 1076
BssMI GATC 2 cut(s) 145, 1083
BssNAI GTATAC 2 cut(s) 812, 1131
BssT1I CCWWGG 3 cut(s) 117, 412, 672
Bst1107I GTATAC 2 cut(s) 812, 1131
Bst2UI CCWGG 1 cut(s) 18
Bst4CI ACNGT 6 cut(s) 142, 439, 887, 1102, 1237, 1322
BstAPI GCANNNNNTGC 1 cut(s) 609
BstBAI YACGTR 1 cut(s) 430
BstC8I GCNNGC 4 cut(s) 165, 421, 526, 904
BstDEI CTNAG 9 cut(s) 44, 365, 565, 620, 649, 920, 994, 1220, 1392
BstF5I GGATG 4 cut(s) 612, 689, 1070, 1150
BstFNI CGCG 1 cut(s) 1111
BstHHI GCGC 1 cut(s) 932
BstKTI GATC 2 cut(s) 148, 1086
BstMAI GTCTC 3 cut(s) 75, 678, 824
BstMBI GATC 2 cut(s) 145, 1083
BstMCI CGRYCG 1 cut(s) 807
BstMWI GCNNNNNNNGC 4 cut(s) 401, 609, 1133, 1154
BstNI CCWGG 1 cut(s) 18
BstNSI RCATGY 2 cut(s) 663, 1212
BstSCI CCNGG 8 cut(s) 16, 390, 1074, 1075, 1183, 1189, 1349, 1450
BstSFI CTRYAG 1 cut(s) 175
BstSLI GKGCMC 1 cut(s) 452
BstUI CGCG 1 cut(s) 1111
BstV1I GCAGC 3 cut(s) 755, 1101, 1169
BstZ17I GTATAC 2 cut(s) 812, 1131
BstZI CGGCCG 1 cut(s) 804
BsuI GTATCC 2 cut(s) 908, 1254
BsuRI GGCC 5 cut(s) 238, 380, 395, 774, 806
BtgZI GCGATG 1 cut(s) 1175
BtrI CACGTC 2 cut(s) 745, 1015
BtsCI GGATG 4 cut(s) 612, 689, 1070, 1150
BtsIMutI CAGTG 1 cut(s) 1068
BveI ACCTGC 1 cut(s) 1321
Cac8I GCNNGC 4 cut(s) 165, 421, 526, 904
CfoI GCGC 1 cut(s) 932
Cfr10I RCCGGY 1 cut(s) 806
Cfr13I GGNCC 4 cut(s) 236, 394, 1098, 1187
Cfr9I CCCGGG 1 cut(s) 1075
CseI GACGC 1 cut(s) 1301
Csp6I GTAC 4 cut(s) 224, 630, 801, 865
CviAII CATG 4 cut(s) 233, 240, 660, 1209
CviQI GTAC 4 cut(s) 224, 630, 801, 865
DdeI CTNAG 9 cut(s) 44, 365, 565, 620, 649, 920, 994, 1220, 1392
DpnI GATC 2 cut(s) 147, 1085
DpnII GATC 2 cut(s) 145, 1083
EaeI YGGCCR 2 cut(s) 772, 804
EagI CGGCCG 1 cut(s) 804
Ecl136II GAGCTC 2 cut(s) 993, 1198
EclXI CGGCCG 1 cut(s) 804
Eco130I CCWWGG 3 cut(s) 117, 412, 672
Eco24I GRGCYC 3 cut(s) 552, 995, 1200
Eco31I GGTCTC 1 cut(s) 824
Eco47I GGWCC 2 cut(s) 1098, 1187
Eco52I CGGCCG 1 cut(s) 804
Eco53kI GAGCTC 2 cut(s) 993, 1198
Eco57I CTGAAG 1 cut(s) 1044
Eco88I CYCGRG 1 cut(s) 1075
EcoICRI GAGCTC 2 cut(s) 993, 1198
EcoRII CCWGG 1 cut(s) 16
EcoT14I CCWWGG 3 cut(s) 117, 412, 672
EcoT22I ATGCAT 1 cut(s) 1150
EcoT38I GRGCYC 3 cut(s) 552, 995, 1200
ErhI CCWWGG 3 cut(s) 117, 412, 672
FaeI CATG 4 cut(s) 236, 243, 663, 1212
FalI AAGNNNNNCTT 2 cut(s) 713, 745
FaqI GGGAC 3 cut(s) 1093, 1111, 1200
FatI CATG 4 cut(s) 232, 239, 659, 1208
FauI CCCGC 2 cut(s) 833, 1134
FblI GTMKAC 3 cut(s) 811, 1130, 1308
Fnu4HI GCNGC 5 cut(s) 769, 772, 1112, 1115, 1158
FokI GGATG 4 cut(s) 599, 676, 1057, 1157
FriOI GRGCYC 3 cut(s) 552, 995, 1200
Fsp4HI GCNGC 5 cut(s) 769, 772, 1112, 1115, 1158
FspBI CTAG 2 cut(s) 293, 1202
GlaI GCGC 1 cut(s) 931
GluI GCNGC 5 cut(s) 769, 772, 1112, 1115, 1158
GsuI CTGGAG 1 cut(s) 1449
HaeIII GGCC 5 cut(s) 238, 380, 395, 774, 806
HapII CCGG 9 cut(s) 392, 486, 807, 1076, 1184, 1190, 1299, 1350, 1452
HgaI GACGC 1 cut(s) 1301
HhaI GCGC 1 cut(s) 932
Hin1II CATG 4 cut(s) 236, 243, 663, 1212
Hin6I GCGC 1 cut(s) 930
HinP1I GCGC 1 cut(s) 930
HinfI GANTC 5 cut(s) 277, 505, 686, 1091, 1437
HpaII CCGG 9 cut(s) 392, 486, 807, 1076, 1184, 1190, 1299, 1350, 1452
HphI GGTGA 3 cut(s) 445, 1205, 1249
Hpy166II GTNNAC 7 cut(s) 226, 316, 812, 1033, 1122, 1131, 1309
Hpy188I TCNGA 3 cut(s) 997, 1248, 1442
Hpy188III TCNNGA 5 cut(s) 215, 293, 330, 622, 1384
Hpy8I GTNNAC 7 cut(s) 226, 316, 812, 1033, 1122, 1131, 1309
Hpy99I CGWCG 2 cut(s) 746, 1274
HpyAV CCTTC 4 cut(s) 40, 199, 370, 779
HpyCH4III ACNGT 6 cut(s) 142, 439, 887, 1102, 1237, 1322
HpyCH4IV ACGT 5 cut(s) 429, 744, 1014, 1241, 1280
HpyCH4V TGCA 3 cut(s) 419, 612, 1148
HpyF10VI GCNNNNNNNGC 4 cut(s) 401, 609, 1133, 1154
HpyF3I CTNAG 9 cut(s) 44, 365, 565, 620, 649, 920, 994, 1220, 1392
HpySE526I ACGT 5 cut(s) 429, 744, 1014, 1241, 1280
Hsp92II CATG 4 cut(s) 236, 243, 663, 1212
HspAI GCGC 1 cut(s) 930
Kzo9I GATC 2 cut(s) 145, 1083
LmnI GCTCC 5 cut(s) 26, 547, 941, 1203, 1453
Lsp1109I GCAGC 3 cut(s) 755, 1101, 1169
LweI GCATC 4 cut(s) 218, 621, 1135, 1351
MaeI CTAG 2 cut(s) 293, 1202
MaeII ACGT 5 cut(s) 429, 744, 1014, 1241, 1280
MaeIII GTNAC 3 cut(s) 433, 1237, 1276
MalI GATC 2 cut(s) 147, 1085
MboI GATC 2 cut(s) 145, 1083
MboII GAAGA 4 cut(s) 352, 751, 868, 1411
MhlI GDGCHC 4 cut(s) 452, 552, 995, 1200
MluCI AATT 8 cut(s) 55, 288, 333, 344, 635, 1050, 1286, 1302
MlyI GAGTC 4 cut(s) 499, 680, 1085, 1446
MmeI TCCRAC 4 cut(s) 640, 1256, 1263, 1420
Mph1103I ATGCAT 1 cut(s) 1150
MseI TTAA 1 cut(s) 731
MslI CAYNNNNRTG 2 cut(s) 231, 1013
MspI CCGG 9 cut(s) 392, 486, 807, 1076, 1184, 1190, 1299, 1350, 1452
MspR9I CCNGG 8 cut(s) 18, 392, 1076, 1077, 1185, 1191, 1351, 1452
MvaI CCWGG 1 cut(s) 18
MvnI CGCG 1 cut(s) 1111
MwoI GCNNNNNNNGC 4 cut(s) 401, 609, 1133, 1154
NciI CCSGG 7 cut(s) 392, 1076, 1077, 1185, 1191, 1351, 1452
NdeII GATC 2 cut(s) 145, 1083
NlaIII CATG 4 cut(s) 236, 243, 663, 1212
NlaIV GGNNCC 4 cut(s) 22, 549, 1099, 1188
NmuCI GTSAC 2 cut(s) 433, 1237
NsiI ATGCAT 1 cut(s) 1150
NspI RCATGY 2 cut(s) 663, 1212
PcsI WCGNNNNNNNCGW 2 cut(s) 1011, 1115
PfeI GAWTC 1 cut(s) 277
PfoI TCCNGGA 1 cut(s) 1183
PkrI GCNGC 5 cut(s) 770, 773, 1113, 1116, 1159
PleI GAGTC 4 cut(s) 499, 680, 1085, 1445
PpsI GAGTC 4 cut(s) 499, 680, 1085, 1445
Ppu21I YACGTR 1 cut(s) 430
Psp124BI GAGCTC 2 cut(s) 995, 1200
Psp6I CCWGG 1 cut(s) 16
PspGI CCWGG 1 cut(s) 16
PspN4I GGNNCC 4 cut(s) 22, 549, 1099, 1188
PspPI GGNCC 4 cut(s) 236, 394, 1098, 1187
PsrI GAACNNNNNNTAC 2 cut(s) 840, 872
RsaI GTAC 4 cut(s) 225, 631, 802, 866
RsaNI GTAC 4 cut(s) 224, 630, 801, 865
RseI CAYNNNNRTG 2 cut(s) 231, 1013
SacI GAGCTC 2 cut(s) 995, 1200
SaqAI TTAA 1 cut(s) 731
SatI GCNGC 5 cut(s) 769, 772, 1112, 1115, 1158
Sau3AI GATC 2 cut(s) 145, 1083
Sau96I GGNCC 4 cut(s) 236, 394, 1098, 1187
SchI GAGTC 4 cut(s) 499, 680, 1085, 1446
ScrFI CCNGG 8 cut(s) 18, 392, 1076, 1077, 1185, 1191, 1351, 1452
SduI GDGCHC 4 cut(s) 452, 552, 995, 1200
SfaNI GCATC 4 cut(s) 218, 621, 1135, 1351
SfcI CTRYAG 1 cut(s) 175
SinI GGWCC 2 cut(s) 1098, 1187
SmaI CCCGGG 1 cut(s) 1077
SmiMI CAYNNNNRTG 2 cut(s) 231, 1013
SmlI CTYRAG 1 cut(s) 988
SmoI CTYRAG 1 cut(s) 988
Sse9I AATT 8 cut(s) 55, 288, 333, 344, 635, 1050, 1286, 1302
SsiI CCGC 7 cut(s) 771, 826, 871, 900, 1111, 1134, 1141
SspMI CTAG 2 cut(s) 293, 1202
SstI GAGCTC 2 cut(s) 995, 1200
StyD4I CCNGG 8 cut(s) 16, 390, 1074, 1075, 1183, 1189, 1349, 1450
StyI CCWWGG 3 cut(s) 117, 412, 672
TaaI ACNGT 6 cut(s) 142, 439, 887, 1102, 1237, 1322
TaiI ACGT 5 cut(s) 432, 747, 1017, 1244, 1283
TaqI TCGA 3 cut(s) 374, 558, 1089
TasI AATT 8 cut(s) 55, 288, 333, 344, 635, 1050, 1286, 1302
TatI WGTACW 2 cut(s) 629, 864
TauI GCSGC 2 cut(s) 774, 1114
TfiI GAWTC 1 cut(s) 277
Tru1I TTAA 1 cut(s) 731
Tru9I TTAA 1 cut(s) 731
TscAI CASTG 1 cut(s) 1068
TseFI GTSAC 2 cut(s) 433, 1237
TseI GCWGC 3 cut(s) 768, 1114, 1157
Tsp45I GTSAC 2 cut(s) 433, 1237
TspDTI ATGAA 3 cut(s) 49, 781, 1059
TspGWI ACGGA 3 cut(s) 140, 1050, 1164
TspMI CCCGGG 1 cut(s) 1075
TspRI CASTG 1 cut(s) 1068
VpaK11BI GGWCC 2 cut(s) 1098, 1187
XapI RAATTY 4 cut(s) 55, 288, 333, 635
XbaI TCTAGA 1 cut(s) 292
XceI RCATGY 2 cut(s) 663, 1212
XcmI CCANNNNNNNNNTGG 1 cut(s) 521
XmaI CCCGGG 1 cut(s) 1075
XmiI GTMKAC 3 cut(s) 811, 1130, 1308
XspI CTAG 2 cut(s) 293, 1202
Zsp2I ATGCAT 1 cut(s) 1150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.