MD08G1050600.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000845
Physical Location & Seq
Reverse (-)
3960195 .. 3962712
2518 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1050600.v1.1.491

Sequence Viewer

Length: 1491 bp
ATGGCAACTCCCATTTCTAGCTCCTCCTCCTTCCTTAGATTTTCCATTGGTTTATCAGCATTTCTATGCCTAATTCTCTGCTCTTTGGCAGTAAGAGATACAAAAACAAACCTCCTTTCACGTACTTACACAGTTGAAGTCATCTCTCTTCTACCAGCAAACACCTGCAGACCCTCCACCAATGGTTATGACAATAAGGCGGCATCCGTACTGAAAGTGGTCCACAAATACGGCCCATGCAACCAAGTCCACCAAGTCAAATCAAACAGTAACCCTTCCGATCAAGATGAATATCACATACAAATCCTCAAGCAAGACGAAGCCCGAGTCAACTCAATCCACTCCCATTTCAACGACTCAAACCGTAATTACAGAATCCCCGACCCACTTGCACAATCAGAAGCCACCGTGATTCCCGCCCAGTCTGCCAGCGTACTTGGTTCTGGAAACTACATCGTCAGTGTCGGCTTGGGCTCGCCCCAGAAGCAACTCTCGCTTGTATTCGACACCGGCAGCAGTCTCACTTGGACACAGTGCCGACCATGTTCTGTATCTTGTTACAAACAGAAAGATCCCATCTTCGACCCTTCTCTCTCTACCTCTTACTCCAACATCTCCTGCAACTCCGCCGCGTGTTCTCAACTTGCAGCCTCCGGTGTCCCCAGCGATTGTTCCGCCTCCACCTGTGTTTACGGCCAACAGTATGGAGACCGCTCCTTCACAATCGGCTTCTTTGCCAGCGAGAAGTTAACTTTGACTTCAACAGATGTTTTTACTGGTTTCGTCTTCGGCTGCGGCCAGAATAACCAAGGCTTATTTAGGGGCATCGCCGGTTTGCTCGGTCTCGGCCGAAGTAAGATCTCCTTTGTTGAACAAACCGCTCAGAAGTACAACAGATTCTTCTCCTACTGCCTCCCTTCAACTTCAAGCTCCACCGGTTACCTCAGCTTGGGCAACTCCGACAACAGAAGTTCAGGTAACGCCGTCAAGTTCACGCCGCTCACCACATTATCTCAAGGCGCGTCGTATTACAGCCTCGGACTGGTGGGCATCAACATCGGTGGGAGTCAATTGCCAATTTCGGCTTCAGTATTTTCGACTTCGGGGACAATCATCGACTCGGGGACGGTCATCACGCGCCTCCCAGCCACAGCGTACACAGCGCTGCGGGACGCATTTCGAGAAGCGATGAAGAGGTACCCAACTGCTAAGTCGATTTCTTCCCTGCTGGACACGTGTTACGACTTCAGTGGATTCAAAACGATAACCTTTCCAACGATAGGGTTTGTGTTTGGCAATGGGGTTACGGTAGATGTGGACGCAACGGGGATAGTAGTCCCGCAGAGTGCTTCGCAGTTTTGCTTGGCGTTTGCCGGAAACAAAGATGATAGGAATGTTGGGATTATTGGGAACGTTCAACAGAAGAGGTTGCAGGTAGTGTATGACGTTGCTGGTGGAAAGGTTGGATTTGCCCCTGCTGGTTGCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

497

Amino Acids

52.84

Weight (kDa)

8.69

Isoelectric Point (pI)

35.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 150 - 493 5.5e-26 Eukaryotic aspartyl protease
TAXi_N PF14543 151 - 319 5.1e-53 Xylanase inhibitor N-terminal
TAXi_C PF14541 344 - 491 4.3e-30 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 173
Acc36I ACCTGC 2 cut(s) 173, 1423
Acc65I GGTACC 1 cut(s) 1197
AccB1I GGYRCC 1 cut(s) 1197
AccBSI CCGCTC 3 cut(s) 714, 881, 1000
AccII CGCG 3 cut(s) 632, 1022, 1138
AclI AACGTT 1 cut(s) 1413
AclWI GGATC 1 cut(s) 566
AcoI YGGCCR 3 cut(s) 694, 796, 847
AcuI CTGAAG 2 cut(s) 1071, 1231
AcvI CACGTG 1 cut(s) 1236
AfaI GTAC 6 cut(s) 124, 210, 435, 890, 1157, 1199
AfeI AGCGCT 1 cut(s) 1164
AfiI CCNNNNNNNGG 3 cut(s) 949, 1042, 1280
AflIII ACRYGT 2 cut(s) 1233, 1235
AgeI ACCGGT 1 cut(s) 935
AgsI TTSAA 8 cut(s) 137, 352, 762, 872, 921, 927, 1258, 1418
AjuI GAANNNNNNNTTGG 2 cut(s) 1069, 1101
AluBI AGCT 3 cut(s) 21, 930, 948
AluI AGCT 3 cut(s) 21, 930, 948
Alw26I GTCTC 3 cut(s) 524, 702, 848
AlwI GGATC 1 cut(s) 566
Ama87I CYCGRG 2 cut(s) 324, 1120
Aor51HI AGCGCT 1 cut(s) 1164
AoxI GGCC 4 cut(s) 232, 694, 796, 847
ApeKI GCWGC 4 cut(s) 513, 647, 792, 1165
ArsI GACNNNNNNTTYG 2 cut(s) 312, 344
AsiGI ACCGGT 1 cut(s) 935
Asp718I GGTACC 1 cut(s) 1197
AspLEI GCGC 3 cut(s) 1022, 1140, 1165
AspS9I GGNCC 2 cut(s) 220, 233
AsuHPI GGTGA 1 cut(s) 994
AvaI CYCGRG 2 cut(s) 324, 1120
AvaII GGWCC 1 cut(s) 220
BanI GGYRCC 1 cut(s) 1197
BanII GRGCYC 1 cut(s) 476
BbrPI CACGTG 1 cut(s) 1236
BbsI GAAGAC 1 cut(s) 778
BbvCI CCTCAGC 1 cut(s) 944
BbvI GCAGC 4 cut(s) 525, 659, 779, 1152
BccI CCATC 1 cut(s) 584
BceAI ACGGC 3 cut(s) 247, 709, 968
BcgI CGANNNNNNTGC 4 cut(s) 371, 405, 1039, 1073
BcoDI GTCTC 3 cut(s) 524, 702, 848
BfaI CTAG 1 cut(s) 18
BfmI CTRYAG 1 cut(s) 166
BfoI RGCGCY 1 cut(s) 1166
BfuAI ACCTGC 2 cut(s) 173, 1423
BglII AGATCT 1 cut(s) 858
BisI GCNGC 8 cut(s) 201, 514, 630, 648, 793, 796, 998, 1166
BlsI GCNGC 8 cut(s) 202, 515, 631, 649, 794, 797, 999, 1167
Bme18I GGWCC 1 cut(s) 220
BmeT110I CYCGRG 2 cut(s) 324, 1120
BmgT120I GGNCC 2 cut(s) 220, 233
BmiI GGNNCC 1 cut(s) 1199
BmrI ACTGGG 1 cut(s) 415
BmsI GCATC 3 cut(s) 212, 834, 1059
BmuI ACTGGG 1 cut(s) 415
BpiI GAAGAC 1 cut(s) 778
Bpu10I CCTNAGC 1 cut(s) 944
BpuEI CTTGAG 2 cut(s) 293, 999
BsaAI YACGTR 2 cut(s) 122, 1236
BsaBI GATNNNNATC 1 cut(s) 291
BsaI GGTCTC 2 cut(s) 702, 848
BsaJI CCNNGG 2 cut(s) 808, 1036
BsaWI WCCGGW 2 cut(s) 653, 935
Bsc4I CCNNNNNNNGG 3 cut(s) 949, 1042, 1280
Bse118I RCCGGY 3 cut(s) 509, 830, 935
Bse1I ACTGG 3 cut(s) 421, 781, 1047
Bse3DI GCAATG 1 cut(s) 1303
Bse8I GATNNNNATC 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 808, 1036
BseGI GGATG 1 cut(s) 203
BseJI GATNNNNATC 1 cut(s) 291
BseLI CCNNNNNNNGG 3 cut(s) 949, 1042, 1280
BseMI GCAATG 1 cut(s) 1303
BseMII CTCAG 2 cut(s) 896, 958
BseNI ACTGG 3 cut(s) 421, 781, 1047
BseRI GAGGAG 2 cut(s) 13, 16
BseX3I CGGCCG 1 cut(s) 847
BseXI GCAGC 4 cut(s) 525, 659, 779, 1152
BseYI CCCAGC 2 cut(s) 662, 1144
Bsh1236I CGCG 3 cut(s) 632, 1022, 1138
Bsh1285I CGRYCG 1 cut(s) 850
BshFI GGCC 4 cut(s) 234, 696, 798, 849
BshNI GGYRCC 1 cut(s) 1197
BshTI ACCGGT 1 cut(s) 935
BsiEI CGRYCG 1 cut(s) 850
BsiHKCI CYCGRG 2 cut(s) 324, 1120
BsiSI CCGG 5 cut(s) 510, 654, 831, 936, 1374
BslFI GGGAC 5 cut(s) 644, 1120, 1138, 1184, 1322
BslI CCNNNNNNNGG 3 cut(s) 949, 1042, 1280
BsmAI GTCTC 3 cut(s) 524, 702, 848
BsmFI GGGAC 5 cut(s) 644, 1120, 1138, 1184, 1322
BsnI GGCC 4 cut(s) 234, 696, 798, 849
Bso31I GGTCTC 2 cut(s) 702, 848
BsoBI CYCGRG 2 cut(s) 324, 1120
Bsp1286I GDGCHC 1 cut(s) 476
Bsp143I GATC 3 cut(s) 280, 571, 858
BspANI GGCC 4 cut(s) 234, 696, 798, 849
BspCNI CTCAG 2 cut(s) 895, 957
BspFNI CGCG 3 cut(s) 632, 1022, 1138
BspLI GGNNCC 1 cut(s) 1199
BspMAI CTGCAG 1 cut(s) 170
BspMI ACCTGC 2 cut(s) 173, 1423
BspPI GGATC 1 cut(s) 566
BspT107I GGYRCC 1 cut(s) 1197
BspTNI GGTCTC 2 cut(s) 702, 848
BsrBI CCGCTC 3 cut(s) 714, 881, 1000
BsrDI GCAATG 1 cut(s) 1303
BsrFI RCCGGY 3 cut(s) 509, 830, 935
BsrI ACTGG 3 cut(s) 421, 781, 1047
BssAI RCCGGY 3 cut(s) 509, 830, 935
BssECI CCNNGG 2 cut(s) 808, 1036
BssMI GATC 3 cut(s) 280, 571, 858
BssT1I CCWWGG 1 cut(s) 808
Bst4CI ACNGT 8 cut(s) 133, 269, 365, 409, 534, 702, 1129, 1309
Bst6I CTCTTC 3 cut(s) 153, 1187, 1418
BstBAI YACGTR 2 cut(s) 122, 1236
BstC8I GCNNGC 3 cut(s) 430, 476, 739
BstDEI CTNAG 4 cut(s) 35, 882, 944, 1209
BstEII GGTNACC 1 cut(s) 938
BstF5I GGATG 1 cut(s) 203
BstFNI CGCG 3 cut(s) 632, 1022, 1138
BstH2I RGCGCY 1 cut(s) 1166
BstHHI GCGC 3 cut(s) 1022, 1140, 1165
BstKTI GATC 3 cut(s) 283, 574, 861
BstMAI GTCTC 3 cut(s) 524, 702, 848
BstMBI GATC 3 cut(s) 280, 571, 858
BstMCI CGRYCG 1 cut(s) 850
BstMWI GCNNNNNNNGC 4 cut(s) 425, 484, 493, 1160
BstPI GGTNACC 1 cut(s) 938
BstSFI CTRYAG 1 cut(s) 166
BstUI CGCG 3 cut(s) 632, 1022, 1138
BstV1I GCAGC 4 cut(s) 525, 659, 779, 1152
BstV2I GAAGAC 1 cut(s) 778
BstX2I RGATCY 2 cut(s) 571, 858
BstXI CCANNNNNNTGG 1 cut(s) 704
BstYI RGATCY 2 cut(s) 571, 858
BstZI CGGCCG 1 cut(s) 847
BsuRI GGCC 4 cut(s) 234, 696, 798, 849
BtgZI GCGATG 2 cut(s) 811, 1202
BtsCI GGATG 1 cut(s) 203
BtsIMutI CAGTG 3 cut(s) 466, 539, 1255
BveI ACCTGC 2 cut(s) 173, 1423
Cac8I GCNNGC 3 cut(s) 430, 476, 739
CfoI GCGC 3 cut(s) 1022, 1140, 1165
Cfr10I RCCGGY 3 cut(s) 509, 830, 935
Cfr13I GGNCC 2 cut(s) 220, 233
CseI GACGC 3 cut(s) 1011, 1181, 1328
Csp6I GTAC 6 cut(s) 123, 209, 434, 889, 1156, 1198
CspAI ACCGGT 1 cut(s) 935
CspCI CAANNNNNGTGG 2 cut(s) 1042, 1077
CviAII CATG 2 cut(s) 237, 543
CviQI GTAC 6 cut(s) 123, 209, 434, 889, 1156, 1198
DdeI CTNAG 4 cut(s) 35, 882, 944, 1209
DpnI GATC 3 cut(s) 282, 573, 860
DpnII GATC 3 cut(s) 280, 571, 858
EaeI YGGCCR 3 cut(s) 694, 796, 847
EagI CGGCCG 1 cut(s) 847
Eam1104I CTCTTC 3 cut(s) 153, 1187, 1418
EarI CTCTTC 3 cut(s) 153, 1187, 1418
EciI GGCGGA 2 cut(s) 616, 664
EclXI CGGCCG 1 cut(s) 847
Eco130I CCWWGG 1 cut(s) 808
Eco24I GRGCYC 1 cut(s) 476
Eco31I GGTCTC 2 cut(s) 702, 848
Eco47I GGWCC 1 cut(s) 220
Eco47III AGCGCT 1 cut(s) 1164
Eco52I CGGCCG 1 cut(s) 847
Eco57I CTGAAG 2 cut(s) 1071, 1231
Eco72I CACGTG 1 cut(s) 1236
Eco88I CYCGRG 2 cut(s) 324, 1120
Eco91I GGTNACC 1 cut(s) 938
EcoO65I GGTNACC 1 cut(s) 938
EcoT14I CCWWGG 1 cut(s) 808
EcoT38I GRGCYC 1 cut(s) 476
ErhI CCWWGG 1 cut(s) 808
FaeI CATG 2 cut(s) 240, 546
FaiI YATR 7 cut(s) 67, 189, 238, 299, 544, 705, 1443
FalI AAGNNNNNCTT 2 cut(s) 848, 880
FaqI GGGAC 5 cut(s) 644, 1120, 1138, 1184, 1322
FatI CATG 2 cut(s) 236, 542
FauI CCCGC 3 cut(s) 424, 1161, 1347
Fnu4HI GCNGC 8 cut(s) 201, 514, 630, 648, 793, 796, 998, 1166
FokI GGATG 1 cut(s) 190
FriOI GRGCYC 1 cut(s) 476
Fsp4HI GCNGC 8 cut(s) 201, 514, 630, 648, 793, 796, 998, 1166
FspBI CTAG 1 cut(s) 18
GlaI GCGC 3 cut(s) 1021, 1139, 1164
GluI GCNGC 8 cut(s) 201, 514, 630, 648, 793, 796, 998, 1166
GsaI CCCAGC 2 cut(s) 666, 1148
HaeII RGCGCY 1 cut(s) 1166
HaeIII GGCC 4 cut(s) 234, 696, 798, 849
HapII CCGG 5 cut(s) 510, 654, 831, 936, 1374
HgaI GACGC 3 cut(s) 1011, 1181, 1328
HhaI GCGC 3 cut(s) 1022, 1140, 1165
Hin1II CATG 2 cut(s) 240, 546
Hin6I GCGC 3 cut(s) 1020, 1138, 1163
HinP1I GCGC 3 cut(s) 1020, 1138, 1163
HincII GTYRAC 2 cut(s) 331, 750
HindII GTYRAC 2 cut(s) 331, 750
HinfI GANTC 8 cut(s) 327, 356, 375, 412, 897, 1066, 1118, 1254
HpaI GTTAAC 1 cut(s) 750
HpaII CCGG 5 cut(s) 510, 654, 831, 936, 1374
HphI GGTGA 1 cut(s) 994
Hpy166II GTNNAC 8 cut(s) 223, 250, 331, 691, 750, 993, 1158, 1318
Hpy188I TCNGA 5 cut(s) 280, 400, 885, 961, 1040
Hpy188III TCNNGA 3 cut(s) 284, 444, 1181
Hpy8I GTNNAC 8 cut(s) 223, 250, 331, 691, 750, 993, 1158, 1318
Hpy99I CGWCG 1 cut(s) 1027
HpyAV CCTTC 5 cut(s) 40, 285, 597, 727, 927
HpyCH4III ACNGT 8 cut(s) 133, 269, 365, 409, 534, 702, 1129, 1309
HpyCH4IV ACGT 4 cut(s) 121, 1235, 1413, 1446
HpyCH4V TGCA 6 cut(s) 168, 240, 392, 621, 647, 1432
HpyF10VI GCNNNNNNNGC 4 cut(s) 425, 484, 493, 1160
HpyF3I CTNAG 4 cut(s) 35, 882, 944, 1209
HpySE526I ACGT 4 cut(s) 121, 1235, 1413, 1446
Hsp92II CATG 2 cut(s) 240, 546
HspAI GCGC 3 cut(s) 1020, 1138, 1163
KpnI GGTACC 1 cut(s) 1201
KspAI GTTAAC 1 cut(s) 750
Kzo9I GATC 3 cut(s) 280, 571, 858
LmnI GCTCC 3 cut(s) 26, 719, 935
Lsp1109I GCAGC 4 cut(s) 525, 659, 779, 1152
LweI GCATC 3 cut(s) 212, 834, 1059
MaeI CTAG 1 cut(s) 18
MaeII ACGT 4 cut(s) 121, 1235, 1413, 1446
MaeIII GTNAC 6 cut(s) 269, 557, 938, 977, 1238, 1303
MalI GATC 3 cut(s) 282, 573, 860
MbiI CCGCTC 3 cut(s) 714, 881, 1000
MboI GATC 3 cut(s) 280, 571, 858
MboII GAAGA 7 cut(s) 140, 571, 778, 892, 1204, 1212, 1435
MfeI CAATTG 1 cut(s) 1070
MflI RGATCY 2 cut(s) 571, 858
MhlI GDGCHC 1 cut(s) 476
MluCI AATT 4 cut(s) 72, 367, 1070, 1077
MlyI GAGTC 4 cut(s) 336, 350, 1075, 1112
MmeI TCCRAC 4 cut(s) 633, 984, 1298, 1444
MseI TTAA 1 cut(s) 749
MslI CAYNNNNRTG 1 cut(s) 64
MspI CCGG 5 cut(s) 510, 654, 831, 936, 1374
MunI CAATTG 1 cut(s) 1070
MvnI CGCG 3 cut(s) 632, 1022, 1138
MwoI GCNNNNNNNGC 4 cut(s) 425, 484, 493, 1160
NdeII GATC 3 cut(s) 280, 571, 858
NlaIII CATG 2 cut(s) 240, 546
NlaIV GGNNCC 1 cut(s) 1199
NmeAIII GCCGAG 1 cut(s) 825
PaqCI CACCTGC 1 cut(s) 173
PfeI GAWTC 4 cut(s) 375, 412, 897, 1254
PinAI ACCGGT 1 cut(s) 935
PkrI GCNGC 8 cut(s) 202, 515, 631, 649, 794, 797, 999, 1167
PleI GAGTC 4 cut(s) 335, 350, 1074, 1112
PmaCI CACGTG 1 cut(s) 1236
PmlI CACGTG 1 cut(s) 1236
PpsI GAGTC 4 cut(s) 335, 350, 1074, 1112
Ppu21I YACGTR 2 cut(s) 122, 1236
Psp1406I AACGTT 1 cut(s) 1413
PspCI CACGTG 1 cut(s) 1236
PspEI GGTNACC 1 cut(s) 938
PspFI CCCAGC 2 cut(s) 662, 1144
PspN4I GGNNCC 1 cut(s) 1199
PspPI GGNCC 2 cut(s) 220, 233
PstI CTGCAG 1 cut(s) 170
PsuI RGATCY 2 cut(s) 571, 858
RsaI GTAC 6 cut(s) 124, 210, 435, 890, 1157, 1199
RsaNI GTAC 6 cut(s) 123, 209, 434, 889, 1156, 1198
RseI CAYNNNNRTG 1 cut(s) 64
SaqAI TTAA 1 cut(s) 749
SatI GCNGC 8 cut(s) 201, 514, 630, 648, 793, 796, 998, 1166
Sau3AI GATC 3 cut(s) 280, 571, 858
Sau96I GGNCC 2 cut(s) 220, 233
SchI GAGTC 4 cut(s) 336, 350, 1075, 1112
SduI GDGCHC 1 cut(s) 476
SfaNI GCATC 3 cut(s) 212, 834, 1059
SfcI CTRYAG 1 cut(s) 166
SinI GGWCC 1 cut(s) 220
SmiMI CAYNNNNRTG 1 cut(s) 64
SmlI CTYRAG 2 cut(s) 308, 1014
SmoI CTYRAG 2 cut(s) 308, 1014
Sse9I AATT 4 cut(s) 72, 367, 1070, 1077
SspMI CTAG 1 cut(s) 18
StyI CCWWGG 1 cut(s) 808
TaaI ACNGT 8 cut(s) 133, 269, 365, 409, 534, 702, 1129, 1309
TaiI ACGT 4 cut(s) 124, 1238, 1416, 1449
TaqI TCGA 6 cut(s) 504, 582, 1097, 1116, 1180, 1214
TaqII GACCGA 1 cut(s) 830
TasI AATT 4 cut(s) 72, 367, 1070, 1077
TatI WGTACW 1 cut(s) 888
TauI GCSGC 4 cut(s) 203, 632, 798, 1000
TfiI GAWTC 4 cut(s) 375, 412, 897, 1254
Tru1I TTAA 1 cut(s) 749
Tru9I TTAA 1 cut(s) 749
TscAI CASTG 3 cut(s) 466, 539, 1255
TseI GCWGC 4 cut(s) 513, 647, 792, 1165
TspDTI ATGAA 2 cut(s) 303, 1205
TspGWI ACGGA 1 cut(s) 196
TspRI CASTG 3 cut(s) 466, 539, 1255
VpaK11BI GGWCC 1 cut(s) 220
XspI CTAG 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.