Rw5G037460

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000845
Physical Location & Seq
Forward (+)
65926666 .. 65928343
1678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G037460.1

Sequence Viewer

Length: 1476 bp
ATGGCAACTCCCATCAGTTCTACCTCCTCCTCCTTCCTCATTATGAGGTATTTCATCAGTTTGTTCGTACTTCTTGGCCTGAATCTGTGCTCTTTGGACAGGGGCTTTGCCTTGGCAGAAGGAACTTCAAAAAGACACCTCCTTCATCAACAATATACCCACACGGTTGATCTCCACTCTCTTCTGCCAGCAACCACCTGCAGCCCCTCCATCAGAGGTAACAAAAGGAAGGCAACTCTTGAAGTGGTACATAAGCATGGGCCATGCTCTCATCTTCCTCAAAACAAAGATACTGCGACGAATCACACTCAAATTCTGGAGCAAGACCAAGCGCGAGTCAACTCAATCAACTCCCGGACTTCCAAGAAACTGAACGGCGTCGAGGATCTAAGCCAATTGGAGGCCACCACACTCCCGGCCAGCCTAGGCGCAGGCAACTACATAGTGACGGTGGGATTAGGCACACCTACAAAACAACTTTCGCTCGAATTTGACACCGGAAGCTACCTCACTTGGACTCAATGTCAACCCTGTGCTGGCTATTGCTACAACCAAACTGATCCAATTTTCGACCCCGCCTCCTCCACCTCATATGCCAACGTTTCTTGTAACAACGCAGAGTGCGTCGAACTCAGCACGTCTACAAATTACAACTCTTGCTTACGAGACACATGCTTATACCGCGTAGGATACGGAGATTCCTCGTCCACCATTGGACTCTTGGCCAAAGACAAGCTGACCTTAACTCCAAACGACGTGTTTGATGGATTCCTCTTTGGCTGCGGTGAAGAAAATGTACACTTCATTGGCCACTCGGCCGGTTTACTTGGTCTCTCCCGCAACAAGTTCTCCATAATCGAACAAACAGGGCAGAAGTACGGCCGCTTCTTCTCCTACTGTCTGCCACCGACCGCAAGCTCCACTGGTTACCTCAGCTTCGGCGCACCCGGTGGAGCTTCCAATGCCGCCATAAAATACACGCGGCTCACTACTTTATCCTCGGGTTTATCGTTTTACGGCCTTGACGTGGTTGGCATCAATGTGAACGGACAAAAGTTATCCATTCCGCCTTCTGTGTTTTCGTCCCCGGGAACGATCATAGACTCCGGGACCGTCATATCGCGGCTTGGGTCCACCGCCTACAGCGCTTTGCGTGACGCCTTTAAGGAAGCAATGAAAAGCTATCCGGCAGCGGCGCCGAATGAGCTCCTCGATACATGCTACGACCTCAGTGGCTATTCAACGGTGACTTATCCGAAAATAGAATTTGTGTTCGGCGACGGGGTTACGTTGGAGTTGGACGCAACCGGCATTGTCTACCTTTTCGACGGTTCGAATAAGCAGGTTTGCTTGGCTTTTGCTGGGAATGATGATGCTAACGACATTGCGATTTTCGGGAATACTCAGCAGAAGAATATGGAGGTGTTGTATGACGTTGCCGGAGGGAGAGTTGGATTTGCTCCTGGCGGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

491

Amino Acids

52.58

Weight (kDa)

5.81

Isoelectric Point (pI)

29.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 146 - 488 1.2e-20 Eukaryotic aspartyl protease
TAXi_N PF14543 147 - 314 2.5e-49 Xylanase inhibitor N-terminal
TAXi_C PF14541 339 - 487 8.3e-29 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 206
Acc36I ACCTGC 2 cut(s) 206, 1333
AccB1I GGYRCC 1 cut(s) 1195
AccI GTMKAC 2 cut(s) 641, 1317
AccII CGCG 4 cut(s) 334, 684, 982, 1123
AclI AACGTT 1 cut(s) 600
AclWI GGATC 2 cut(s) 393, 554
AcoI YGGCCR 5 cut(s) 417, 723, 808, 816, 880
AcsI RAATTY 3 cut(s) 312, 488, 1265
AcyI GRCGYC 3 cut(s) 378, 1158, 1196
AdeI CACNNNGTG 1 cut(s) 950
AfaI GTAC 4 cut(s) 69, 249, 798, 878
AfeI AGCGCT 1 cut(s) 1147
AfiI CCNNNNNNNGG 3 cut(s) 400, 536, 1027
AflIII ACRYGT 1 cut(s) 756
AgsI TTSAA 3 cut(s) 129, 242, 1242
AhdI GACNNNNNGTC 1 cut(s) 522
AjiI CACGTC 3 cut(s) 639, 757, 1027
AjnI CCWGG 1 cut(s) 1462
AluBI AGCT 7 cut(s) 504, 736, 918, 936, 956, 1182, 1207
AluI AGCT 7 cut(s) 504, 736, 918, 936, 956, 1182, 1207
Alw21I GWGCWC 2 cut(s) 92, 1209
Alw26I GTCTC 2 cut(s) 660, 836
AlwI GGATC 2 cut(s) 393, 554
Ama87I CYCGRG 2 cut(s) 1000, 1087
Aor51HI AGCGCT 1 cut(s) 1147
AoxI GGCC 9 cut(s) 76, 260, 402, 417, 723, 808, 816, 880, 1018
ApeKI GCWGC 3 cut(s) 201, 780, 1190
ApoI RAATTY 3 cut(s) 312, 488, 1265
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
AspA2I CCTAGG 1 cut(s) 424
AspLEI GCGC 5 cut(s) 334, 431, 944, 1148, 1198
AspS9I GGNCC 3 cut(s) 260, 1110, 1131
AsuC2I CCSGG 6 cut(s) 355, 416, 948, 1088, 1089, 1108
AsuHPI GGTGA 2 cut(s) 797, 1258
AsuII TTCGAA 1 cut(s) 1334
AvaI CYCGRG 2 cut(s) 1000, 1087
AvaII GGWCC 2 cut(s) 1110, 1131
AvrII CCTAGG 1 cut(s) 424
BalI TGGCCA 2 cut(s) 725, 810
BanI GGYRCC 1 cut(s) 1195
BanII GRGCYC 1 cut(s) 1209
BarI GAAGNNNNNNTAC 4 cut(s) 780, 812, 869, 901
Bbv12I GWGCWC 2 cut(s) 92, 1209
BbvCI CCTCAGC 1 cut(s) 932
BbvI GCAGC 3 cut(s) 213, 767, 1202
BccI CCATC 3 cut(s) 20, 218, 758
BceAI ACGGC 3 cut(s) 391, 895, 1033
BcgI CGANNNNNNTGC 2 cut(s) 654, 688
BciT130I CCWGG 1 cut(s) 1464
BciVI GTATCC 1 cut(s) 683
BcnI CCSGG 6 cut(s) 355, 416, 948, 1088, 1089, 1108
BcoDI GTCTC 2 cut(s) 660, 836
BfaI CTAG 1 cut(s) 425
BfmI CTRYAG 2 cut(s) 199, 1141
BfoI RGCGCY 2 cut(s) 1149, 1199
BfuAI ACCTGC 2 cut(s) 206, 1333
BfuI GTATCC 1 cut(s) 683
BisI GCNGC 8 cut(s) 202, 781, 883, 966, 983, 1124, 1191, 1194
BlnI CCTAGG 1 cut(s) 424
BlsI GCNGC 8 cut(s) 203, 782, 884, 967, 984, 1125, 1192, 1195
Bme1390I CCNGG 7 cut(s) 355, 416, 948, 1088, 1089, 1108, 1464
Bme18I GGWCC 2 cut(s) 1110, 1131
BmeRI GACNNNNNGTC 1 cut(s) 522
BmeT110I CYCGRG 2 cut(s) 1000, 1087
BmgBI CACGTC 3 cut(s) 639, 757, 1027
BmgT120I GGNCC 3 cut(s) 260, 1110, 1131
BmiI GGNNCC 3 cut(s) 1111, 1132, 1197
BmrFI CCNGG 7 cut(s) 355, 416, 948, 1088, 1089, 1108, 1464
BmsI GCATC 2 cut(s) 1044, 1363
BpmI CTGGAG 1 cut(s) 338
Bpu10I CCTNAGC 1 cut(s) 932
Bpu14I TTCGAA 1 cut(s) 1334
BpuMI CCSGG 6 cut(s) 355, 416, 948, 1088, 1089, 1108
BsaHI GRCGYC 3 cut(s) 378, 1158, 1196
BsaI GGTCTC 1 cut(s) 836
BsaJI CCNNGG 5 cut(s) 111, 424, 999, 1086, 1087
BsaWI WCCGGW 1 cut(s) 497
Bsc4I CCNNNNNNNGG 3 cut(s) 400, 536, 1027
Bse118I RCCGGY 2 cut(s) 818, 1307
Bse1I ACTGG 1 cut(s) 928
Bse3DI GCAATG 2 cut(s) 1179, 1383
BseBI CCWGG 1 cut(s) 1464
BseDI CCNNGG 5 cut(s) 111, 424, 999, 1086, 1087
BseLI CCNNNNNNNGG 3 cut(s) 400, 536, 1027
BseMI GCAATG 2 cut(s) 1179, 1383
BseMII CTCAG 4 cut(s) 646, 946, 1243, 1418
BseNI ACTGG 1 cut(s) 928
BseRI GAGGAG 4 cut(s) 16, 19, 571, 1199
BseX3I CGGCCG 2 cut(s) 816, 880
BseXI GCAGC 3 cut(s) 213, 767, 1202
BseYI CCCAGC 1 cut(s) 1361
Bsh1236I CGCG 4 cut(s) 334, 684, 982, 1123
Bsh1285I CGRYCG 3 cut(s) 819, 883, 912
BshFI GGCC 9 cut(s) 78, 262, 404, 419, 725, 810, 818, 882, 1020
BshNI GGYRCC 1 cut(s) 1195
BsiEI CGRYCG 3 cut(s) 819, 883, 912
BsiHKAI GWGCWC 2 cut(s) 92, 1209
BsiHKCI CYCGRG 2 cut(s) 1000, 1087
BslFI GGGAC 2 cut(s) 1069, 1123
BslI CCNNNNNNNGG 3 cut(s) 400, 536, 1027
BsmAI GTCTC 2 cut(s) 660, 836
BsmFI GGGAC 2 cut(s) 1069, 1123
BsnI GGCC 9 cut(s) 78, 262, 404, 419, 725, 810, 818, 882, 1020
Bso31I GGTCTC 1 cut(s) 836
BsoBI CYCGRG 2 cut(s) 1000, 1087
Bsp119I TTCGAA 1 cut(s) 1334
Bsp1286I GDGCHC 2 cut(s) 92, 1209
Bsp1407I TGTACA 1 cut(s) 796
Bsp143I GATC 4 cut(s) 169, 385, 559, 1095
BspANI GGCC 9 cut(s) 78, 262, 404, 419, 725, 810, 818, 882, 1020
BspCNI CTCAG 4 cut(s) 645, 945, 1242, 1417
BspFNI CGCG 4 cut(s) 334, 684, 982, 1123
BspLI GGNNCC 3 cut(s) 1111, 1132, 1197
BspMAI CTGCAG 1 cut(s) 203
BspMI ACCTGC 2 cut(s) 206, 1333
BspPI GGATC 2 cut(s) 393, 554
BspT104I TTCGAA 1 cut(s) 1334
BspT107I GGYRCC 1 cut(s) 1195
BspTNI GGTCTC 1 cut(s) 836
BsrDI GCAATG 2 cut(s) 1179, 1383
BsrFI RCCGGY 2 cut(s) 818, 1307
BsrGI TGTACA 1 cut(s) 796
BsrI ACTGG 1 cut(s) 928
BssAI RCCGGY 2 cut(s) 818, 1307
BssECI CCNNGG 5 cut(s) 111, 424, 999, 1086, 1087
BssMI GATC 4 cut(s) 169, 385, 559, 1095
BssNI GRCGYC 3 cut(s) 378, 1158, 1196
BssT1I CCWWGG 2 cut(s) 111, 424
Bst2UI CCWGG 1 cut(s) 1464
Bst4CI ACNGT 6 cut(s) 166, 451, 899, 1114, 1246, 1331
Bst6I CTCTTC 1 cut(s) 186
BstACI GRCGYC 3 cut(s) 378, 1158, 1196
BstAUI TGTACA 1 cut(s) 796
BstBI TTCGAA 1 cut(s) 1334
BstC8I GCNNGC 5 cut(s) 189, 421, 433, 538, 916
BstDEI CTNAG 5 cut(s) 389, 632, 932, 1229, 1404
BstEII GGTNACC 1 cut(s) 926
BstFNI CGCG 4 cut(s) 334, 684, 982, 1123
BstH2I RGCGCY 2 cut(s) 1149, 1199
BstHHI GCGC 5 cut(s) 334, 431, 944, 1148, 1198
BstKTI GATC 4 cut(s) 172, 388, 562, 1098
BstMAI GTCTC 2 cut(s) 660, 836
BstMBI GATC 4 cut(s) 169, 385, 559, 1095
BstMCI CGRYCG 3 cut(s) 819, 883, 912
BstMWI GCNNNNNNNGC 4 cut(s) 681, 962, 1145, 1204
BstNI CCWGG 1 cut(s) 1464
BstNSI RCATGY 2 cut(s) 675, 1221
BstPI GGTNACC 1 cut(s) 926
BstSCI CCNGG 7 cut(s) 353, 414, 946, 1086, 1087, 1106, 1462
BstSFI CTRYAG 2 cut(s) 199, 1141
BstUI CGCG 4 cut(s) 334, 684, 982, 1123
BstV1I GCAGC 3 cut(s) 213, 767, 1202
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BstZI CGGCCG 2 cut(s) 816, 880
BsuI GTATCC 1 cut(s) 683
BsuRI GGCC 9 cut(s) 78, 262, 404, 419, 725, 810, 818, 882, 1020
BtrI CACGTC 3 cut(s) 639, 757, 1027
BtsIMutI CAGTG 2 cut(s) 921, 1237
BveI ACCTGC 2 cut(s) 206, 1333
Cac8I GCNNGC 5 cut(s) 189, 421, 433, 538, 916
CfoI GCGC 5 cut(s) 334, 431, 944, 1148, 1198
Cfr10I RCCGGY 2 cut(s) 818, 1307
Cfr13I GGNCC 3 cut(s) 260, 1110, 1131
Cfr9I CCCGGG 1 cut(s) 1087
CseI GACGC 4 cut(s) 367, 613, 1166, 1310
Csp6I GTAC 4 cut(s) 68, 248, 797, 877
CviAII CATG 4 cut(s) 257, 264, 672, 1218
CviQI GTAC 4 cut(s) 68, 248, 797, 877
DdeI CTNAG 5 cut(s) 389, 632, 932, 1229, 1404
DinI GGCGCC 1 cut(s) 1197
DpnI GATC 4 cut(s) 171, 387, 561, 1097
DpnII GATC 4 cut(s) 169, 385, 559, 1095
DraIII CACNNNGTG 1 cut(s) 950
DriI GACNNNNNGTC 1 cut(s) 522
EaeI YGGCCR 5 cut(s) 417, 723, 808, 816, 880
EagI CGGCCG 2 cut(s) 816, 880
Eam1104I CTCTTC 1 cut(s) 186
Eam1105I GACNNNNNGTC 1 cut(s) 522
EarI CTCTTC 1 cut(s) 186
EciI GGCGGA 1 cut(s) 1056
Ecl136II GAGCTC 1 cut(s) 1207
EclXI CGGCCG 2 cut(s) 816, 880
Eco130I CCWWGG 2 cut(s) 111, 424
Eco24I GRGCYC 1 cut(s) 1209
Eco31I GGTCTC 1 cut(s) 836
Eco47I GGWCC 2 cut(s) 1110, 1131
Eco47III AGCGCT 1 cut(s) 1147
Eco52I CGGCCG 2 cut(s) 816, 880
Eco53kI GAGCTC 1 cut(s) 1207
Eco88I CYCGRG 2 cut(s) 1000, 1087
Eco91I GGTNACC 1 cut(s) 926
EcoICRI GAGCTC 1 cut(s) 1207
EcoO65I GGTNACC 1 cut(s) 926
EcoRII CCWGG 1 cut(s) 1462
EcoT14I CCWWGG 2 cut(s) 111, 424
EcoT38I GRGCYC 1 cut(s) 1209
EgeI GGCGCC 1 cut(s) 1197
EheI GGCGCC 1 cut(s) 1197
ErhI CCWWGG 2 cut(s) 111, 424
FaeI CATG 4 cut(s) 260, 267, 675, 1221
FalI AAGNNNNNCTT 2 cut(s) 725, 757
FaqI GGGAC 2 cut(s) 1069, 1123
FatI CATG 4 cut(s) 256, 263, 671, 1217
FauI CCCGC 2 cut(s) 583, 845
FauNDI CATATG 1 cut(s) 592
FblI GTMKAC 2 cut(s) 641, 1317
Fnu4HI GCNGC 8 cut(s) 202, 781, 883, 966, 983, 1124, 1191, 1194
FriOI GRGCYC 1 cut(s) 1209
Fsp4HI GCNGC 8 cut(s) 202, 781, 883, 966, 983, 1124, 1191, 1194
FspBI CTAG 1 cut(s) 425
GlaI GCGC 5 cut(s) 333, 430, 943, 1147, 1197
GluI GCNGC 8 cut(s) 202, 781, 883, 966, 983, 1124, 1191, 1194
GsaI CCCAGC 1 cut(s) 1365
GsuI CTGGAG 1 cut(s) 338
HaeII RGCGCY 2 cut(s) 1149, 1199
HaeIII GGCC 9 cut(s) 78, 262, 404, 419, 725, 810, 818, 882, 1020
HgaI GACGC 4 cut(s) 367, 613, 1166, 1310
HhaI GCGC 5 cut(s) 334, 431, 944, 1148, 1198
Hin1I GRCGYC 3 cut(s) 378, 1158, 1196
Hin1II CATG 4 cut(s) 260, 267, 675, 1221
Hin6I GCGC 5 cut(s) 332, 429, 942, 1146, 1196
HinP1I GCGC 5 cut(s) 332, 429, 942, 1146, 1196
HincII GTYRAC 2 cut(s) 340, 527
HindII GTYRAC 2 cut(s) 340, 527
HinfI GANTC 8 cut(s) 82, 301, 336, 517, 698, 717, 768, 1103
HphI GGTGA 2 cut(s) 797, 1258
Hpy166II GTNNAC 9 cut(s) 340, 527, 642, 708, 799, 824, 1045, 1134, 1318
Hpy188I TCNGA 2 cut(s) 215, 1257
Hpy188III TCNNGA 3 cut(s) 239, 317, 1396
Hpy8I GTNNAC 9 cut(s) 340, 527, 642, 708, 799, 824, 1045, 1134, 1318
Hpy99I CGWCG 6 cut(s) 301, 383, 629, 758, 1283, 1331
HpyAV CCTTC 5 cut(s) 43, 113, 152, 223, 1080
HpyCH4III ACNGT 6 cut(s) 166, 451, 899, 1114, 1246, 1331
HpyCH4IV ACGT 6 cut(s) 600, 638, 756, 1026, 1289, 1434
HpyCH4V TGCA 1 cut(s) 201
HpyF10VI GCNNNNNNNGC 4 cut(s) 681, 962, 1145, 1204
HpyF3I CTNAG 5 cut(s) 389, 632, 932, 1229, 1404
HpySE526I ACGT 6 cut(s) 600, 638, 756, 1026, 1289, 1434
Hsp92I GRCGYC 3 cut(s) 378, 1158, 1196
Hsp92II CATG 4 cut(s) 260, 267, 675, 1221
HspAI GCGC 5 cut(s) 332, 429, 942, 1146, 1196
KasI GGCGCC 1 cut(s) 1195
Kzo9I GATC 4 cut(s) 169, 385, 559, 1095
LmnI GCTCC 5 cut(s) 319, 923, 953, 1212, 1465
Lsp1109I GCAGC 3 cut(s) 213, 767, 1202
LweI GCATC 2 cut(s) 1044, 1363
MaeI CTAG 1 cut(s) 425
MaeII ACGT 6 cut(s) 600, 638, 756, 1026, 1289, 1434
MaeIII GTNAC 7 cut(s) 218, 445, 608, 926, 1154, 1246, 1285
MalI GATC 4 cut(s) 171, 387, 561, 1097
MboI GATC 4 cut(s) 169, 385, 559, 1095
MboII GAAGA 5 cut(s) 173, 266, 800, 880, 1423
MfeI CAATTG 1 cut(s) 395
MflI RGATCY 1 cut(s) 385
MhlI GDGCHC 2 cut(s) 92, 1209
MlsI TGGCCA 2 cut(s) 725, 810
MluCI AATT 6 cut(s) 312, 395, 488, 564, 646, 1265
MluNI TGGCCA 2 cut(s) 725, 810
Mly113I GGCGCC 1 cut(s) 1196
MlyI GAGTC 4 cut(s) 345, 511, 711, 1097
MmeI TCCRAC 3 cut(s) 1272, 1278, 1432
Mox20I TGGCCA 2 cut(s) 725, 810
MscI TGGCCA 2 cut(s) 725, 810
MseI TTAA 2 cut(s) 743, 1164
MslI CAYNNNNRTG 2 cut(s) 255, 1040
Msp20I TGGCCA 2 cut(s) 725, 810
MspA1I CMGCKG 1 cut(s) 1193
MspR9I CCNGG 7 cut(s) 355, 416, 948, 1088, 1089, 1108, 1464
MunI CAATTG 1 cut(s) 395
MvaI CCWGG 1 cut(s) 1464
MvnI CGCG 4 cut(s) 334, 684, 982, 1123
MwoI GCNNNNNNNGC 4 cut(s) 681, 962, 1145, 1204
NarI GGCGCC 1 cut(s) 1196
NciI CCSGG 6 cut(s) 355, 416, 948, 1088, 1089, 1108
NdeI CATATG 1 cut(s) 592
NdeII GATC 4 cut(s) 169, 385, 559, 1095
NlaIII CATG 4 cut(s) 260, 267, 675, 1221
NlaIV GGNNCC 3 cut(s) 1111, 1132, 1197
NmeAIII GCCGAG 1 cut(s) 794
NmuCI GTSAC 3 cut(s) 445, 1154, 1246
NspI RCATGY 2 cut(s) 675, 1221
NspV TTCGAA 1 cut(s) 1334
PaqCI CACCTGC 1 cut(s) 206
PcsI WCGNNNNNNNCGW 3 cut(s) 621, 1007, 1023
PfeI GAWTC 4 cut(s) 82, 301, 698, 768
PfoI TCCNGGA 2 cut(s) 353, 1106
PkrI GCNGC 8 cut(s) 203, 782, 884, 967, 984, 1125, 1192, 1195
PleI GAGTC 4 cut(s) 344, 511, 711, 1097
PluTI GGCGCC 1 cut(s) 1199
PpsI GAGTC 4 cut(s) 344, 511, 711, 1097
Psp124BI GAGCTC 1 cut(s) 1209
Psp1406I AACGTT 1 cut(s) 600
Psp6I CCWGG 1 cut(s) 1462
PspEI GGTNACC 1 cut(s) 926
PspFI CCCAGC 1 cut(s) 1361
PspGI CCWGG 1 cut(s) 1462
PspN4I GGNNCC 3 cut(s) 1111, 1132, 1197
PspPI GGNCC 3 cut(s) 260, 1110, 1131
PstI CTGCAG 1 cut(s) 203
PsuI RGATCY 1 cut(s) 385
RsaI GTAC 4 cut(s) 69, 249, 798, 878
RsaNI GTAC 4 cut(s) 68, 248, 797, 877
RseI CAYNNNNRTG 2 cut(s) 255, 1040
SacI GAGCTC 1 cut(s) 1209
SaqAI TTAA 2 cut(s) 743, 1164
SatI GCNGC 8 cut(s) 202, 781, 883, 966, 983, 1124, 1191, 1194
Sau3AI GATC 4 cut(s) 169, 385, 559, 1095
Sau96I GGNCC 3 cut(s) 260, 1110, 1131
SchI GAGTC 4 cut(s) 345, 511, 711, 1097
ScrFI CCNGG 7 cut(s) 355, 416, 948, 1088, 1089, 1108, 1464
SduI GDGCHC 2 cut(s) 92, 1209
SfaNI GCATC 2 cut(s) 1044, 1363
SfcI CTRYAG 2 cut(s) 199, 1141
SfoI GGCGCC 1 cut(s) 1197
SfuI TTCGAA 1 cut(s) 1334
SinI GGWCC 2 cut(s) 1110, 1131
SmaI CCCGGG 1 cut(s) 1089
SmiMI CAYNNNNRTG 2 cut(s) 255, 1040
Sse9I AATT 6 cut(s) 312, 395, 488, 564, 646, 1265
SspDI GGCGCC 1 cut(s) 1195
SspMI CTAG 1 cut(s) 425
SstI GAGCTC 1 cut(s) 1209
StyD4I CCNGG 7 cut(s) 353, 414, 946, 1086, 1087, 1106, 1462
StyI CCWWGG 2 cut(s) 111, 424
TaaI ACNGT 6 cut(s) 166, 451, 899, 1114, 1246, 1331
TaiI ACGT 6 cut(s) 603, 641, 759, 1029, 1292, 1437
TaqI TCGA 8 cut(s) 381, 486, 570, 627, 858, 1212, 1326, 1334
TasI AATT 6 cut(s) 312, 395, 488, 564, 646, 1265
TatI WGTACW 1 cut(s) 796
TauI GCSGC 5 cut(s) 885, 968, 985, 1126, 1196
TfiI GAWTC 4 cut(s) 82, 301, 698, 768
Tru1I TTAA 2 cut(s) 743, 1164
Tru9I TTAA 2 cut(s) 743, 1164
TscAI CASTG 2 cut(s) 928, 1237
TseFI GTSAC 3 cut(s) 445, 1154, 1246
TseI GCWGC 3 cut(s) 201, 780, 1190
Tsp45I GTSAC 3 cut(s) 445, 1154, 1246
TspDTI ATGAA 4 cut(s) 43, 134, 793, 1190
TspGWI ACGGA 2 cut(s) 708, 1062
TspMI CCCGGG 1 cut(s) 1087
TspRI CASTG 2 cut(s) 928, 1237
VpaK11BI GGWCC 2 cut(s) 1110, 1131
XapI RAATTY 3 cut(s) 312, 488, 1265
XceI RCATGY 2 cut(s) 675, 1221
XcmI CCANNNNNNNNNTGG 1 cut(s) 718
XmaI CCCGGG 1 cut(s) 1087
XmaJI CCTAGG 1 cut(s) 424
XmiI GTMKAC 2 cut(s) 641, 1317
XspI CTAG 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.