pycom15g04110

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000845
Physical Location & Seq
Forward (+)
2489530 .. 2491438
1909 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g04110.2

Sequence Viewer

Length: 1467 bp
ATGGCAACTCCCATTTCTATCTCCCCCTCCTTCCTCAGGGGTTTCTTTGGTTGCTTTGTGTTTTTTATCTGCGTAATTCTCTGCTCGCCGGAGAAGGGCCTTGCTTCGGCAGAAGGAGATACAAAAACTGACCTTCTTCCACATACTCACACAGTTGAAGTAAACTCTCTGCAACCAGCAACCACCTGCATCCTCTCCACCCAAGGTCCTTACAATAACAAGGAATCAGTATTGGAAGTGGTACACAAGCATGGGCCATGCAAACAACACAGATCACAAAGTAACCCCACTGATCAGGATCGCACTCAAATCCTCAAGCAAGACGAAGCCCGAGTCAGCTCAATTCACTACCGTCTTAACTCACTCACACAACCTGACGCCGCCACCAATACCATACCCGTCCAGTACGGTCTGCAACTTGGATCATCCAACTACATTGTAACAGTGGGCCTCGGCACACCGGCCAAAAACCTCTCCCTCCTTATCGACACAGGCAGCGACCTAACTTGGACACAATGCCGACCGTGTGTCCCATCTTGTTACAAACAGAAAGAGCCAATGTTCGACCCCTCCCACTCCACCTCATACTCCAACATCTCCTGTGAGTCCGCTGCGTGTTCTCAGCTCACAGTGGCCGGCATCGGCCGCGACTGCTCTGCAGCCACGTGCACCTACAGCGCCAAGTATTTAGACAACTCTTCCTCACTGGGCTTCTTCGGCAATGACAAGCTAACAATAACACCGACGGATGCTTACGATGATTTCGATTTTGGCTGCGCGCAAAACAGCGAAGTCCAGTTCGGGGCCGCCGCCGGTTTGCTCGGTCTGGGCCGGACCAGCATCTCCTTCGTCGAACAAACCTCCCAGAAATACAAACGAGTGTTCTCCTACTGTCTACCCTCCACTTCAAGCTCCACCGGCTACCTCAAATTCGGCCCCGACGGAGGCTCCGGTAACGCCGTCAAGTTCACGCCGCTCAAAACTTTATCCCAAAGCGCTACATTTTACGCCCTTGACCTAGTTGGAATCACCGTCGGTAGCCGTCAACTGAAGATTTCAGCCTCTATCTTTTCAAAGTCGGGGACAGTCATCGACTCGGGGACGGTGATCACGCGCCTTCCGGCCACCCCGTATGCAGCATTACGCGCGGCTTTTAGAAAAGCGATGGAGAAGTACCCTTTTGCTAAGTCGAAGTCTGCCCTACTAGACACTTGCTACGACTTTAGTGGACTTAAAACGGTGTCGTACCCGAAGATAGGGTTTGTATTTGGAGGTGGGGTTACGGTAGACTTGGACGCGACAGGGGTACTGTTCCAGACTAGTGGTTCGCAGTTTTGCTTGGCGTTTGCCGGAAACAAGGACGATAGTAGCGTCGGGATTATCGGCAACGTTCAGCAGAAGAGGCTGCAAGTGGTGTACGACGTTGCTGGGGGAAAGATCGGATTTGGCCCTGCAGGTTGCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

489

Amino Acids

52.0

Weight (kDa)

8.21

Isoelectric Point (pI)

38.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 194
Acc36I ACCTGC 2 cut(s) 194, 1445
AccBSI CCGCTC 1 cut(s) 976
AccI GTMKAC 2 cut(s) 895, 1287
AccII CGCG 6 cut(s) 648, 779, 1114, 1146, 1148, 1298
AciI CCGC 7 cut(s) 381, 609, 646, 807, 810, 974, 1148
AclI AACGTT 1 cut(s) 1389
AclWI GGATC 2 cut(s) 306, 430
AcoI YGGCCR 4 cut(s) 462, 633, 643, 1122
AcsI RAATTY 1 cut(s) 929
AcuI CTGAAG 1 cut(s) 1070
AcvI CACGTG 1 cut(s) 666
AcyI GRCGYC 1 cut(s) 378
AfaI GTAC 6 cut(s) 243, 407, 1175, 1247, 1308, 1418
AfeI AGCGCT 1 cut(s) 997
AfiI CCNNNNNNNGG 6 cut(s) 36, 95, 106, 802, 944, 1256
AgsI TTSAA 3 cut(s) 158, 909, 1074
AhdI GACNNNNNGTC 1 cut(s) 527
AhlI ACTAGT 1 cut(s) 1319
AluBI AGCT 4 cut(s) 339, 625, 730, 912
AluI AGCT 4 cut(s) 339, 625, 730, 912
Alw21I GWGCWC 1 cut(s) 671
Alw44I GTGCAC 1 cut(s) 667
AlwI GGATC 2 cut(s) 306, 430
Ama87I CYCGRG 2 cut(s) 330, 1096
Aor51HI AGCGCT 1 cut(s) 997
ApaLI GTGCAC 1 cut(s) 667
ApeKI GCWGC 6 cut(s) 495, 611, 659, 774, 1136, 1405
ApoI RAATTY 1 cut(s) 929
ArsI GACNNNNNNTTYG 4 cut(s) 318, 350, 1309, 1341
AspLEI GCGC 6 cut(s) 680, 779, 781, 998, 1116, 1148
AspS9I GGNCC 9 cut(s) 97, 206, 254, 448, 804, 829, 834, 935, 1448
AsuHPI GGTGA 2 cut(s) 1021, 1117
AvaI CYCGRG 2 cut(s) 330, 1096
AvaII GGWCC 2 cut(s) 206, 834
AxyI CCTNAGG 1 cut(s) 35
BaeGI GKGCMC 1 cut(s) 671
BbrPI CACGTG 1 cut(s) 666
Bbv12I GWGCWC 1 cut(s) 671
BbvI GCAGC 6 cut(s) 507, 598, 671, 761, 1148, 1392
BccI CCATC 2 cut(s) 541, 1159
BceAI ACGGC 2 cut(s) 944, 1026
BcgI CGANNNNNNTGC 4 cut(s) 638, 672, 829, 863
BclI TGATCA 2 cut(s) 292, 1107
BcuI ACTAGT 1 cut(s) 1319
BfaI CTAG 3 cut(s) 1019, 1205, 1320
BfmI CTRYAG 3 cut(s) 657, 673, 1452
BfoI RGCGCY 2 cut(s) 681, 999
BfuAI ACCTGC 2 cut(s) 194, 1445
Bme18I GGWCC 2 cut(s) 206, 834
BmeRI GACNNNNNGTC 1 cut(s) 527
BmeT110I CYCGRG 2 cut(s) 330, 1096
BmgT120I GGNCC 9 cut(s) 97, 206, 254, 448, 804, 829, 834, 935, 1448
BmiI GGNNCC 3 cut(s) 805, 937, 949
BmrI ACTGGG 1 cut(s) 716
BmsI GCATC 4 cut(s) 198, 648, 739, 849
BmuI ACTGGG 1 cut(s) 716
BpuEI CTTGAG 1 cut(s) 299
BsaAI YACGTR 1 cut(s) 666
BsaBI GATNNNNATC 1 cut(s) 297
BsaHI GRCGYC 1 cut(s) 378
BsaJI CCNNGG 2 cut(s) 202, 451
BsaWI WCCGGW 1 cut(s) 950
BsaXI ACNNNNNCTCC 4 cut(s) 932, 962, 1263, 1293
Bsc4I CCNNNNNNNGG 6 cut(s) 36, 95, 106, 802, 944, 1256
Bse118I RCCGGY 4 cut(s) 460, 635, 812, 917
Bse1I ACTGG 3 cut(s) 403, 711, 796
Bse21I CCTNAGG 1 cut(s) 35
Bse3DI GCAATG 1 cut(s) 727
Bse8I GATNNNNATC 1 cut(s) 297
BseDI CCNNGG 2 cut(s) 202, 451
BseGI GGATG 3 cut(s) 189, 425, 754
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 6 cut(s) 36, 95, 106, 802, 944, 1256
BseMI GCAATG 1 cut(s) 727
BseMII CTCAG 2 cut(s) 49, 635
BseNI ACTGG 3 cut(s) 403, 711, 796
BsePI GCGCGC 1 cut(s) 777
BseSI GKGCMC 1 cut(s) 671
BseX3I CGGCCG 1 cut(s) 643
BseXI GCAGC 6 cut(s) 507, 598, 671, 761, 1148, 1392
BseYI CCCAGC 1 cut(s) 1427
Bsh1236I CGCG 6 cut(s) 648, 779, 1114, 1146, 1148, 1298
Bsh1285I CGRYCG 2 cut(s) 524, 646
BsiEI CGRYCG 2 cut(s) 524, 646
BsiHKAI GWGCWC 1 cut(s) 671
BsiHKCI CYCGRG 2 cut(s) 330, 1096
BsiSI CCGG 9 cut(s) 89, 461, 636, 813, 832, 918, 951, 1121, 1350
BslFI GGGAC 3 cut(s) 515, 1096, 1114
BslI CCNNNNNNNGG 6 cut(s) 36, 95, 106, 802, 944, 1256
BsmFI GGGAC 3 cut(s) 515, 1096, 1114
BsoBI CYCGRG 2 cut(s) 330, 1096
Bsp1286I GDGCHC 1 cut(s) 671
Bsp143I GATC 6 cut(s) 272, 292, 298, 422, 1107, 1437
BspACI CCGC 7 cut(s) 381, 609, 646, 807, 810, 974, 1148
BspCNI CTCAG 2 cut(s) 48, 634
BspFNI CGCG 6 cut(s) 648, 779, 1114, 1146, 1148, 1298
BspLI GGNNCC 3 cut(s) 805, 937, 949
BspMAI CTGCAG 2 cut(s) 661, 1456
BspMI ACCTGC 2 cut(s) 194, 1445
BspPI GGATC 2 cut(s) 306, 430
BsrBI CCGCTC 1 cut(s) 976
BsrDI GCAATG 1 cut(s) 727
BsrFI RCCGGY 4 cut(s) 460, 635, 812, 917
BsrI ACTGG 3 cut(s) 403, 711, 796
BssAI RCCGGY 4 cut(s) 460, 635, 812, 917
BssECI CCNNGG 2 cut(s) 202, 451
BssHII GCGCGC 1 cut(s) 777
BssMI GATC 6 cut(s) 272, 292, 298, 422, 1107, 1437
BssNI GRCGYC 1 cut(s) 378
BssT1I CCWWGG 1 cut(s) 202
Bst6I CTCTTC 2 cut(s) 703, 1394
BstACI GRCGYC 1 cut(s) 378
BstBAI YACGTR 1 cut(s) 666
BstC8I GCNNGC 3 cut(s) 86, 637, 779
BstDEI CTNAG 3 cut(s) 35, 621, 1185
BstENI CCTNNNNNAGG 1 cut(s) 34
BstF5I GGATG 3 cut(s) 189, 425, 754
BstFNI CGCG 6 cut(s) 648, 779, 1114, 1146, 1148, 1298
BstH2I RGCGCY 2 cut(s) 681, 999
BstHHI GCGC 6 cut(s) 680, 779, 781, 998, 1116, 1148
BstKTI GATC 6 cut(s) 275, 295, 301, 425, 1110, 1440
BstMBI GATC 6 cut(s) 272, 292, 298, 422, 1107, 1437
BstMCI CGRYCG 2 cut(s) 524, 646
BstMWI GCNNNNNNNGC 8 cut(s) 645, 651, 675, 717, 837, 918, 1145, 1402
BstSFI CTRYAG 3 cut(s) 657, 673, 1452
BstSLI GKGCMC 1 cut(s) 671
BstUI CGCG 6 cut(s) 648, 779, 1114, 1146, 1148, 1298
BstV1I GCAGC 6 cut(s) 507, 598, 671, 761, 1148, 1392
BstXI CCANNNNNNTGG 1 cut(s) 1322
BstZI CGGCCG 1 cut(s) 643
Bsu36I CCTNAGG 1 cut(s) 35
BtgZI GCGATG 1 cut(s) 1178
BtsCI GGATG 3 cut(s) 189, 425, 754
BtsIMutI CAGTG 4 cut(s) 288, 450, 636, 704
BveI ACCTGC 2 cut(s) 194, 1445
Cac8I GCNNGC 3 cut(s) 86, 637, 779
CfoI GCGC 6 cut(s) 680, 779, 781, 998, 1116, 1148
Cfr10I RCCGGY 4 cut(s) 460, 635, 812, 917
Cfr13I GGNCC 9 cut(s) 97, 206, 254, 448, 804, 829, 834, 935, 1448
CseI GACGC 3 cut(s) 386, 1304, 1360
Csp6I GTAC 6 cut(s) 242, 406, 1174, 1246, 1307, 1417
CviAII CATG 2 cut(s) 251, 258
CviQI GTAC 6 cut(s) 242, 406, 1174, 1246, 1307, 1417
DdeI CTNAG 3 cut(s) 35, 621, 1185
DpnI GATC 6 cut(s) 274, 294, 300, 424, 1109, 1439
DpnII GATC 6 cut(s) 272, 292, 298, 422, 1107, 1437
DriI GACNNNNNGTC 1 cut(s) 527
EaeI YGGCCR 4 cut(s) 462, 633, 643, 1122
EagI CGGCCG 1 cut(s) 643
Eam1104I CTCTTC 2 cut(s) 703, 1394
Eam1105I GACNNNNNGTC 1 cut(s) 527
EarI CTCTTC 2 cut(s) 703, 1394
EclXI CGGCCG 1 cut(s) 643
Eco130I CCWWGG 1 cut(s) 202
Eco47I GGWCC 2 cut(s) 206, 834
Eco47III AGCGCT 1 cut(s) 997
Eco52I CGGCCG 1 cut(s) 643
Eco57I CTGAAG 1 cut(s) 1070
Eco72I CACGTG 1 cut(s) 666
Eco81I CCTNAGG 1 cut(s) 35
Eco88I CYCGRG 2 cut(s) 330, 1096
EcoNI CCTNNNNNAGG 1 cut(s) 34
EcoO109I RGGNCCY 2 cut(s) 97, 206
EcoT14I CCWWGG 1 cut(s) 202
ErhI CCWWGG 1 cut(s) 202
FaeI CATG 2 cut(s) 254, 261
FaiI YATR 6 cut(s) 144, 252, 259, 395, 586, 1134
FaqI GGGAC 3 cut(s) 515, 1096, 1114
FatI CATG 2 cut(s) 250, 257
FbaI TGATCA 2 cut(s) 292, 1107
FblI GTMKAC 2 cut(s) 895, 1287
FokI GGATG 3 cut(s) 176, 412, 761
FspBI CTAG 3 cut(s) 1019, 1205, 1320
GlaI GCGC 6 cut(s) 679, 778, 780, 997, 1115, 1147
GsaI CCCAGC 1 cut(s) 1431
HaeII RGCGCY 2 cut(s) 681, 999
HapII CCGG 9 cut(s) 89, 461, 636, 813, 832, 918, 951, 1121, 1350
HgaI GACGC 3 cut(s) 386, 1304, 1360
HhaI GCGC 6 cut(s) 680, 779, 781, 998, 1116, 1148
Hin1I GRCGYC 1 cut(s) 378
Hin1II CATG 2 cut(s) 254, 261
Hin6I GCGC 6 cut(s) 678, 777, 779, 996, 1114, 1146
HinP1I GCGC 6 cut(s) 678, 777, 779, 996, 1114, 1146
HincII GTYRAC 1 cut(s) 1046
HindII GTYRAC 1 cut(s) 1046
HinfI GANTC 5 cut(s) 224, 333, 605, 1026, 1094
HpaII CCGG 9 cut(s) 89, 461, 636, 813, 832, 918, 951, 1121, 1350
HphI GGTGA 2 cut(s) 1021, 1117
Hpy166II GTNNAC 9 cut(s) 163, 244, 669, 896, 969, 1046, 1229, 1288, 1417
Hpy188I TCNGA 1 cut(s) 1442
Hpy188III TCNNGA 3 cut(s) 296, 1315, 1375
Hpy8I GTNNAC 9 cut(s) 163, 244, 669, 896, 969, 1046, 1229, 1288, 1417
Hpy99I CGWCG 6 cut(s) 748, 854, 944, 1037, 1376, 1424
HpyAV CCTTC 6 cut(s) 40, 88, 107, 143, 856, 1127
HpyCH4IV ACGT 3 cut(s) 665, 1389, 1422
HpyCH4V TGCA 9 cut(s) 172, 189, 261, 415, 659, 669, 1136, 1408, 1454
HpyF10VI GCNNNNNNNGC 8 cut(s) 645, 651, 675, 717, 837, 918, 1145, 1402
HpyF3I CTNAG 3 cut(s) 35, 621, 1185
HpySE526I ACGT 3 cut(s) 665, 1389, 1422
Hsp92I GRCGYC 1 cut(s) 378
Hsp92II CATG 2 cut(s) 254, 261
HspAI GCGC 6 cut(s) 678, 777, 779, 996, 1114, 1146
KroI GCCGGC 1 cut(s) 635
KroNI GCCGGC 1 cut(s) 637
Ksp22I TGATCA 2 cut(s) 292, 1107
Kzo9I GATC 6 cut(s) 272, 292, 298, 422, 1107, 1437
LmnI GCTCC 2 cut(s) 917, 953
Lsp1109I GCAGC 6 cut(s) 507, 598, 671, 761, 1148, 1392
LweI GCATC 4 cut(s) 198, 648, 739, 849
MaeI CTAG 3 cut(s) 1019, 1205, 1320
MaeII ACGT 3 cut(s) 665, 1389, 1422
MaeIII GTNAC 5 cut(s) 281, 439, 539, 953, 1279
MalI GATC 6 cut(s) 274, 294, 300, 424, 1109, 1439
MbiI CCGCTC 1 cut(s) 976
MboI GATC 6 cut(s) 272, 292, 298, 422, 1107, 1437
MboII GAAGA 6 cut(s) 128, 690, 706, 1063, 1264, 1411
MhlI GDGCHC 1 cut(s) 671
MluCI AATT 3 cut(s) 75, 342, 929
MlyI GAGTC 3 cut(s) 342, 614, 1088
MmeI TCCRAC 3 cut(s) 453, 615, 1003
MroNI GCCGGC 1 cut(s) 635
MseI TTAA 2 cut(s) 357, 1233
MslI CAYNNNNRTG 1 cut(s) 249
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 9 cut(s) 89, 461, 636, 813, 832, 918, 951, 1121, 1350
MvnI CGCG 6 cut(s) 648, 779, 1114, 1146, 1148, 1298
MwoI GCNNNNNNNGC 8 cut(s) 645, 651, 675, 717, 837, 918, 1145, 1402
NaeI GCCGGC 1 cut(s) 637
NdeII GATC 6 cut(s) 272, 292, 298, 422, 1107, 1437
NgoMIV GCCGGC 1 cut(s) 635
NlaIII CATG 2 cut(s) 254, 261
NlaIV GGNNCC 3 cut(s) 805, 937, 949
NmeAIII GCCGAG 1 cut(s) 432
PaqCI CACCTGC 1 cut(s) 194
PauI GCGCGC 1 cut(s) 777
PcsI WCGNNNNNNNCGW 2 cut(s) 762, 1368
PdiI GCCGGC 1 cut(s) 637
PfeI GAWTC 2 cut(s) 224, 1026
PleI GAGTC 3 cut(s) 341, 613, 1088
PmaCI CACGTG 1 cut(s) 666
PmlI CACGTG 1 cut(s) 666
PpsI GAGTC 3 cut(s) 341, 613, 1088
Ppu21I YACGTR 1 cut(s) 666
PpuMI RGGWCCY 1 cut(s) 206
Psp1406I AACGTT 1 cut(s) 1389
Psp5II RGGWCCY 1 cut(s) 206
PspCI CACGTG 1 cut(s) 666
PspFI CCCAGC 1 cut(s) 1427
PspN4I GGNNCC 3 cut(s) 805, 937, 949
PspPI GGNCC 9 cut(s) 97, 206, 254, 448, 804, 829, 834, 935, 1448
PspPPI RGGWCCY 1 cut(s) 206
PstI CTGCAG 2 cut(s) 661, 1456
PteI GCGCGC 1 cut(s) 777
RsaI GTAC 6 cut(s) 243, 407, 1175, 1247, 1308, 1418
RsaNI GTAC 6 cut(s) 242, 406, 1174, 1246, 1307, 1417
RseI CAYNNNNRTG 1 cut(s) 249
SaqAI TTAA 2 cut(s) 357, 1233
Sau3AI GATC 6 cut(s) 272, 292, 298, 422, 1107, 1437
Sau96I GGNCC 9 cut(s) 97, 206, 254, 448, 804, 829, 834, 935, 1448
SbfI CCTGCAGG 1 cut(s) 1456
SchI GAGTC 3 cut(s) 342, 614, 1088
SdaI CCTGCAGG 1 cut(s) 1456
SduI GDGCHC 1 cut(s) 671
SfaNI GCATC 4 cut(s) 198, 648, 739, 849
SfcI CTRYAG 3 cut(s) 657, 673, 1452
SinI GGWCC 2 cut(s) 206, 834
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
SpeI ACTAGT 1 cut(s) 1319
Sse8387I CCTGCAGG 1 cut(s) 1456
Sse9I AATT 3 cut(s) 75, 342, 929
SsiI CCGC 7 cut(s) 381, 609, 646, 807, 810, 974, 1148
SspMI CTAG 3 cut(s) 1019, 1205, 1320
StyI CCWWGG 1 cut(s) 202
TaiI ACGT 3 cut(s) 668, 1392, 1425
TaqI TCGA 6 cut(s) 486, 564, 765, 852, 1092, 1190
TaqII GACCGA 1 cut(s) 812
TasI AATT 3 cut(s) 75, 342, 929
TauI GCSGC 6 cut(s) 383, 648, 809, 812, 976, 1151
TfiI GAWTC 2 cut(s) 224, 1026
Tru1I TTAA 2 cut(s) 357, 1233
Tru9I TTAA 2 cut(s) 357, 1233
TscAI CASTG 4 cut(s) 295, 450, 636, 711
TseI GCWGC 6 cut(s) 495, 611, 659, 774, 1136, 1405
TspGWI ACGGA 2 cut(s) 761, 957
TspRI CASTG 4 cut(s) 295, 450, 636, 711
VneI GTGCAC 1 cut(s) 667
VpaK11BI GGWCC 2 cut(s) 206, 834
XagI CCTNNNNNAGG 1 cut(s) 34
XapI RAATTY 1 cut(s) 929
XmiI GTMKAC 2 cut(s) 895, 1287
XspI CTAG 3 cut(s) 1019, 1205, 1320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.