Prupe.1G087300_v2.0.a1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000845
Physical Location & Seq
Forward (+)
6495720 .. 6497840
2121 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G087300.1

Sequence Viewer

Length: 1494 bp
ATGGAAACTCCCAATATTTCTAGCTCCTCCTTCCTCTGGTATTTCATTCGTTTCCTTGTACTTGTCTGCCTAATTCTTTGCTCTTTGGAGACATGCTTTACCTTGGAAGAAAGTGATGTAAAAAGCCACCTTGGTCTTGAATATAGTCATACTGTTCAAGTCAAGTCTCTGCTACCAGCAACCACTTGCAGCCCCTCCACCCAAGGTCATGACAAGAAGGCATCAGTACTTAAAGTGGTACACAAGCATGGCCCTTGCTCCCACTTCAACCAATCACACAAAGAAAATGCTCCCACTCACACTCAAATCCTCGAGCAAGACCAAGCCCGAGTGAATTCAATCCACTCTCGGCGTAATCCCAAGAAAGCAATTGACCGCCGTGGCCAAAGACAGAGGGACAGCACCACTGTCCCGGCCTTGTCCGGGAGTACAGTGGGTGCGGCCGACTACATTGTGACGGTGGGCCTAGGCACACCTAAGAAACAACTTTCACTCACATTTGACACAGGAAGCGACCTCACTTGGACTCAATGTCGGCCCTGCATTAGGCCTTGCTACAAACAAGTGGACCCCATCTTTGACCCTTCAGTCTCCAGCTCATATGCCAACGTGTCTTGCAATTCTGTGGTGTGCTCTCAACTCAAATCTGGTACGGGCTATGCACCCGCTTGTGCAACATCCACATGCGTATACACCACAACATACGGAGACGGCTCCATCTCCGTCGGATTCTTCGCCAAAGAAACCATTACTTTAACTTCGACGGATGTGTTCGACGGGTTCCTCTTTGGCTGCGGCAAAGTCAACCAAGGCCTCTTTCGTGGCTCTGCGGGGTTGCTTGGCCTTGGTCGCGACAGTATTTCCTTTGTTGAACAAACTGCGGCCAAATACGGCCGCTTTTTCTCCTATTGTCTACCCTCCACCTCAAGCTCCACCGGTTTCCTTAGCTTTGGCAGAGAGAAGCGCGGCAGAGCTTCCAAGGGAGTGAAGTTCACGCGGCTCGCCACCCTCCCACAAAAGGAGTACTCATCGTTATATGGCATTAACCTGGTTGGTATCACTGTGGGCGGACGTAAGTTACCCATTTCAACTTCAGTTTTTAAAACTTCCGGGACGATAATCGACTCGGGTACGGTTATAACACGGCTGCCACCACAGGCGTACAGCGCTTTGCGGGCTGCATTTCAGCAAGGGATGAAAAGCTACCCGAGAGCTCCGGCAATTTCAATATTGGATACATGCTATGACTTTAGTAGTTATGAGACAGTTACTATTCCGAAAATAGCAATTTCTTTTAAGGGTGGATTGACGTTGGATTTGGATGGTACTGGCATAATATATCCGGTGACTGCTTCCCAAGCTTGTTTGGCTTTTGCTGCAAATAAGAATGACAGTGACATTGCCGTATTTGGGAATGTTCAGCAGCTGAAATTCGAGGTGGTGTATGACGTTGATAAAGGGAAGCTTGGATTTGCCTCTGGAGGTTGCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

498

Amino Acids

53.56

Weight (kDa)

9.01

Isoelectric Point (pI)

32.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1139
AasI GACNNNNNNGTC 1 cut(s) 587
AccI GTMKAC 2 cut(s) 690, 913
AccII CGCG 3 cut(s) 852, 966, 997
AcoI YGGCCR 4 cut(s) 382, 441, 882, 892
AcsI RAATTY 2 cut(s) 334, 1430
AcuI CTGAAG 2 cut(s) 570, 1077
AfaI GTAC 9 cut(s) 60, 228, 240, 430, 652, 1025, 1132, 1163, 1327
AfeI AGCGCT 1 cut(s) 1168
AfiI CCNNNNNNNGG 5 cut(s) 36, 423, 546, 1018, 1410
AflIII ACRYGT 1 cut(s) 609
AgeI ACCGGT 1 cut(s) 935
AgsI TTSAA 7 cut(s) 140, 158, 268, 339, 872, 1089, 1227
AhdI GACNNNNNGTC 1 cut(s) 531
AjnI CCWGG 1 cut(s) 1047
AjuI GAANNNNNNNTTGG 2 cut(s) 801, 833
Alw21I GWGCWC 2 cut(s) 635, 1216
Alw26I GTCTC 5 cut(s) 83, 171, 595, 702, 1256
AlwNI CAGNNNCTG 1 cut(s) 1426
Ama87I CYCGRG 4 cut(s) 311, 327, 1126, 1207
Aor51HI AGCGCT 1 cut(s) 1168
ApeKI GCWGC 6 cut(s) 189, 792, 1147, 1178, 1376, 1423
ApoI RAATTY 2 cut(s) 334, 1430
ArsI GACNNNNNNTTYG 4 cut(s) 560, 592, 1300, 1332
AsiGI ACCGGT 1 cut(s) 935
AspA2I CCTAGG 1 cut(s) 466
AspLEI GCGC 2 cut(s) 966, 1169
AspS9I GGNCC 4 cut(s) 251, 463, 537, 568
AsuC2I CCSGG 3 cut(s) 413, 424, 1111
AsuHPI GGTGA 1 cut(s) 1357
AvaI CYCGRG 4 cut(s) 311, 327, 1126, 1207
AvaII GGWCC 1 cut(s) 568
AvrII CCTAGG 1 cut(s) 466
BalI TGGCCA 1 cut(s) 384
BanII GRGCYC 1 cut(s) 1216
Bbv12I GWGCWC 2 cut(s) 635, 1216
BbvI GCAGC 6 cut(s) 201, 779, 1134, 1165, 1363, 1435
BccI CCATC 3 cut(s) 581, 725, 1316
BceAI ACGGC 5 cut(s) 363, 727, 907, 1160, 1388
BciT130I CCWGG 1 cut(s) 1049
BciVI GTATCC 1 cut(s) 1228
BcnI CCSGG 3 cut(s) 413, 424, 1111
BcoDI GTCTC 5 cut(s) 83, 171, 595, 702, 1256
BfaI CTAG 2 cut(s) 21, 467
BfoI RGCGCY 1 cut(s) 1170
BfuI GTATCC 1 cut(s) 1228
BlnI CCTAGG 1 cut(s) 466
BmcAI AGTACT 2 cut(s) 228, 1025
Bme1390I CCNGG 4 cut(s) 413, 424, 1049, 1111
Bme18I GGWCC 1 cut(s) 568
BmeRI GACNNNNNGTC 1 cut(s) 531
BmeT110I CYCGRG 4 cut(s) 311, 327, 1126, 1207
BmgT120I GGNCC 4 cut(s) 251, 463, 537, 568
BmiI GGNNCC 3 cut(s) 570, 715, 782
BmrFI CCNGG 4 cut(s) 413, 424, 1049, 1111
BmsI GCATC 1 cut(s) 230
BplI GAGNNNNNCTC 1 cut(s) 1473
BpmI CTGGAG 1 cut(s) 577
Bpu10I CCTNAGC 1 cut(s) 944
BpuEI CTTGAG 1 cut(s) 910
BpuMI CCSGG 3 cut(s) 413, 424, 1111
BsaJI CCNNGG 8 cut(s) 102, 130, 202, 379, 466, 808, 844, 978
BsaWI WCCGGW 2 cut(s) 935, 1342
Bsc4I CCNNNNNNNGG 5 cut(s) 36, 423, 546, 1018, 1410
Bse118I RCCGGY 1 cut(s) 935
Bse1I ACTGG 1 cut(s) 1333
Bse3DI GCAATG 1 cut(s) 1398
BseBI CCWGG 1 cut(s) 1049
BseDI CCNNGG 8 cut(s) 102, 130, 202, 379, 466, 808, 844, 978
BseGI GGATG 4 cut(s) 677, 772, 1200, 1327
BseLI CCNNNNNNNGG 5 cut(s) 36, 423, 546, 1018, 1410
BseMI GCAATG 1 cut(s) 1398
BseNI ACTGG 1 cut(s) 1333
BseRI GAGGAG 1 cut(s) 16
BseX3I CGGCCG 2 cut(s) 441, 892
BseXI GCAGC 6 cut(s) 201, 779, 1134, 1165, 1363, 1435
Bsh1236I CGCG 3 cut(s) 852, 966, 997
Bsh1285I CGRYCG 2 cut(s) 444, 895
BshTI ACCGGT 1 cut(s) 935
BsiEI CGRYCG 2 cut(s) 444, 895
BsiHKAI GWGCWC 2 cut(s) 635, 1216
BsiHKCI CYCGRG 4 cut(s) 311, 327, 1126, 1207
BsiSI CCGG 6 cut(s) 413, 423, 936, 1110, 1217, 1343
BslFI GGGAC 3 cut(s) 395, 410, 1126
BslI CCNNNNNNNGG 5 cut(s) 36, 423, 546, 1018, 1410
BsmAI GTCTC 5 cut(s) 83, 171, 595, 702, 1256
BsmBI CGTCTC 1 cut(s) 702
BsmFI GGGAC 3 cut(s) 395, 410, 1126
BsoBI CYCGRG 4 cut(s) 311, 327, 1126, 1207
Bsp1286I GDGCHC 2 cut(s) 635, 1216
Bsp68I TCGCGA 1 cut(s) 852
BspFNI CGCG 3 cut(s) 852, 966, 997
BspHI TCATGA 1 cut(s) 208
BspLI GGNNCC 3 cut(s) 570, 715, 782
BsrDI GCAATG 1 cut(s) 1398
BsrFI RCCGGY 1 cut(s) 935
BsrI ACTGG 1 cut(s) 1333
BssAI RCCGGY 1 cut(s) 935
BssECI CCNNGG 8 cut(s) 102, 130, 202, 379, 466, 808, 844, 978
BssNAI GTATAC 1 cut(s) 691
BssT1I CCWWGG 7 cut(s) 102, 130, 202, 466, 808, 844, 978
Bst1107I GTATAC 1 cut(s) 691
Bst2UI CCWGG 1 cut(s) 1049
Bst4CI ACNGT 9 cut(s) 154, 409, 433, 460, 857, 1063, 1135, 1267, 1394
BstC8I GCNNGC 2 cut(s) 1002, 1176
BstDEI CTNAG 2 cut(s) 477, 944
BstDSI CCRYGG 1 cut(s) 379
BstENI CCTNNNNNAGG 1 cut(s) 544
BstF5I GGATG 4 cut(s) 677, 772, 1200, 1327
BstFNI CGCG 3 cut(s) 852, 966, 997
BstH2I RGCGCY 1 cut(s) 1170
BstHHI GCGC 2 cut(s) 966, 1169
BstMAI GTCTC 5 cut(s) 83, 171, 595, 702, 1256
BstMCI CGRYCG 2 cut(s) 444, 895
BstMWI GCNNNNNNNGC 6 cut(s) 849, 1166, 1175, 1358, 1367, 1376
BstNI CCWGG 1 cut(s) 1049
BstNSI RCATGY 3 cut(s) 96, 687, 1242
BstSCI CCNGG 4 cut(s) 411, 422, 1047, 1109
BstUI CGCG 3 cut(s) 852, 966, 997
BstV1I GCAGC 6 cut(s) 201, 779, 1134, 1165, 1363, 1435
BstZ17I GTATAC 1 cut(s) 691
BstZI CGGCCG 2 cut(s) 441, 892
BsuI GTATCC 1 cut(s) 1228
BtgI CCRYGG 1 cut(s) 379
BtsCI GGATG 4 cut(s) 677, 772, 1200, 1327
BtsIMutI CAGTG 4 cut(s) 405, 438, 1059, 1399
BtuMI TCGCGA 1 cut(s) 852
Cac8I GCNNGC 2 cut(s) 1002, 1176
CaiI CAGNNNCTG 1 cut(s) 1426
CciI TCATGA 1 cut(s) 208
CfoI GCGC 2 cut(s) 966, 1169
Cfr10I RCCGGY 1 cut(s) 935
Cfr13I GGNCC 4 cut(s) 251, 463, 537, 568
CsiI ACCWGGT 1 cut(s) 1047
Csp6I GTAC 9 cut(s) 59, 227, 239, 429, 651, 1024, 1131, 1162, 1326
CspAI ACCGGT 1 cut(s) 935
CviAII CATG 5 cut(s) 93, 209, 248, 684, 1239
CviQI GTAC 9 cut(s) 59, 227, 239, 429, 651, 1024, 1131, 1162, 1326
DdeI CTNAG 2 cut(s) 477, 944
DraI TTTAAA 1 cut(s) 1102
DrdI GACNNNNNNGTC 1 cut(s) 587
DriI GACNNNNNGTC 1 cut(s) 531
DseDI GACNNNNNNGTC 1 cut(s) 587
EaeI YGGCCR 4 cut(s) 382, 441, 882, 892
EagI CGGCCG 2 cut(s) 441, 892
Eam1105I GACNNNNNGTC 1 cut(s) 531
EciI GGCGGA 1 cut(s) 1083
Ecl136II GAGCTC 1 cut(s) 1214
EclXI CGGCCG 2 cut(s) 441, 892
Eco130I CCWWGG 7 cut(s) 102, 130, 202, 466, 808, 844, 978
Eco147I AGGCCT 2 cut(s) 550, 813
Eco24I GRGCYC 1 cut(s) 1216
Eco47I GGWCC 1 cut(s) 568
Eco47III AGCGCT 1 cut(s) 1168
Eco52I CGGCCG 2 cut(s) 441, 892
Eco53kI GAGCTC 1 cut(s) 1214
Eco57I CTGAAG 2 cut(s) 570, 1077
Eco88I CYCGRG 4 cut(s) 311, 327, 1126, 1207
EcoICRI GAGCTC 1 cut(s) 1214
EcoNI CCTNNNNNAGG 1 cut(s) 544
EcoRI GAATTC 1 cut(s) 334
EcoRII CCWGG 1 cut(s) 1047
EcoT14I CCWWGG 7 cut(s) 102, 130, 202, 466, 808, 844, 978
EcoT38I GRGCYC 1 cut(s) 1216
ErhI CCWWGG 7 cut(s) 102, 130, 202, 466, 808, 844, 978
Esp3I CGTCTC 1 cut(s) 702
FaeI CATG 5 cut(s) 96, 212, 251, 687, 1242
FalI AAGNNNNNCTT 2 cut(s) 1449, 1481
FaqI GGGAC 3 cut(s) 395, 410, 1126
FatI CATG 5 cut(s) 92, 208, 247, 683, 1238
FauI CCCGC 3 cut(s) 673, 823, 1167
FauNDI CATATG 1 cut(s) 601
FblI GTMKAC 2 cut(s) 690, 913
FokI GGATG 4 cut(s) 664, 779, 1207, 1334
FriOI GRGCYC 1 cut(s) 1216
FspBI CTAG 2 cut(s) 21, 467
GlaI GCGC 2 cut(s) 965, 1168
GsuI CTGGAG 1 cut(s) 577
HaeII RGCGCY 1 cut(s) 1170
HapII CCGG 6 cut(s) 413, 423, 936, 1110, 1217, 1343
HhaI GCGC 2 cut(s) 966, 1169
Hin1II CATG 5 cut(s) 96, 212, 251, 687, 1242
Hin6I GCGC 2 cut(s) 964, 1167
HinP1I GCGC 2 cut(s) 964, 1167
HincII GTYRAC 1 cut(s) 805
HindII GTYRAC 1 cut(s) 805
HindIII AAGCTT 2 cut(s) 1359, 1463
HinfI GANTC 3 cut(s) 526, 729, 1124
HpaII CCGG 6 cut(s) 413, 423, 936, 1110, 1217, 1343
HphI GGTGA 1 cut(s) 1357
Hpy166II GTNNAC 6 cut(s) 241, 568, 691, 805, 914, 993
Hpy188I TCNGA 3 cut(s) 728, 1278, 1493
Hpy188III TCNNGA 4 cut(s) 137, 209, 851, 1479
Hpy8I GTNNAC 6 cut(s) 241, 568, 691, 805, 914, 993
Hpy99I CGWCG 3 cut(s) 728, 766, 779
HpyAV CCTTC 3 cut(s) 40, 211, 594
HpyCH4III ACNGT 9 cut(s) 154, 409, 433, 460, 857, 1063, 1135, 1267, 1394
HpyCH4IV ACGT 4 cut(s) 609, 1072, 1310, 1449
HpyCH4V TGCA 7 cut(s) 189, 543, 618, 662, 674, 1181, 1379
HpyF10VI GCNNNNNNNGC 6 cut(s) 849, 1166, 1175, 1358, 1367, 1376
HpyF3I CTNAG 2 cut(s) 477, 944
HpySE526I ACGT 4 cut(s) 609, 1072, 1310, 1449
Hsp92II CATG 5 cut(s) 96, 212, 251, 687, 1242
HspAI GCGC 2 cut(s) 964, 1167
LmnI GCTCC 6 cut(s) 29, 263, 295, 719, 935, 1219
Lsp1109I GCAGC 6 cut(s) 201, 779, 1134, 1165, 1363, 1435
LweI GCATC 1 cut(s) 230
MabI ACCWGGT 1 cut(s) 1047
MaeI CTAG 2 cut(s) 21, 467
MaeII ACGT 4 cut(s) 609, 1072, 1310, 1449
MaeIII GTNAC 5 cut(s) 454, 1077, 1267, 1345, 1394
MboII GAAGA 2 cut(s) 119, 724
MfeI CAATTG 1 cut(s) 369
MhlI GDGCHC 2 cut(s) 635, 1216
MlsI TGGCCA 1 cut(s) 384
MluCI AATT 7 cut(s) 72, 334, 369, 619, 1221, 1287, 1430
MluNI TGGCCA 1 cut(s) 384
MlyI GAGTC 2 cut(s) 520, 1118
MmeI TCCRAC 2 cut(s) 706, 1293
Mox20I TGGCCA 1 cut(s) 384
MscI TGGCCA 1 cut(s) 384
MseI TTAA 5 cut(s) 231, 755, 1044, 1101, 1296
MslI CAYNNNNRTG 2 cut(s) 246, 682
Msp20I TGGCCA 1 cut(s) 384
MspA1I CMGCKG 1 cut(s) 1426
MspI CCGG 6 cut(s) 413, 423, 936, 1110, 1217, 1343
MspR9I CCNGG 4 cut(s) 413, 424, 1049, 1111
MunI CAATTG 1 cut(s) 369
MvaI CCWGG 1 cut(s) 1049
MvnI CGCG 3 cut(s) 852, 966, 997
MwoI GCNNNNNNNGC 6 cut(s) 849, 1166, 1175, 1358, 1367, 1376
NciI CCSGG 3 cut(s) 413, 424, 1111
NdeI CATATG 1 cut(s) 601
NlaIII CATG 5 cut(s) 96, 212, 251, 687, 1242
NlaIV GGNNCC 3 cut(s) 570, 715, 782
NmeAIII GCCGAG 1 cut(s) 328
NmuCI GTSAC 3 cut(s) 454, 1345, 1394
NruI TCGCGA 1 cut(s) 852
NspI RCATGY 3 cut(s) 96, 687, 1242
PaeR7I CTCGAG 1 cut(s) 311
PagI TCATGA 1 cut(s) 208
PceI AGGCCT 2 cut(s) 550, 813
PfeI GAWTC 1 cut(s) 729
PfoI TCCNGGA 2 cut(s) 422, 1109
PinAI ACCGGT 1 cut(s) 935
PleI GAGTC 2 cut(s) 520, 1118
PpsI GAGTC 2 cut(s) 520, 1118
PsiI TTATAA 1 cut(s) 1139
Psp124BI GAGCTC 1 cut(s) 1216
Psp6I CCWGG 1 cut(s) 1047
PspGI CCWGG 1 cut(s) 1047
PspN4I GGNNCC 3 cut(s) 570, 715, 782
PspPI GGNCC 4 cut(s) 251, 463, 537, 568
PspXI VCTCGAGB 1 cut(s) 311
PstNI CAGNNNCTG 1 cut(s) 1426
PvuII CAGCTG 1 cut(s) 1426
RruI TCGCGA 1 cut(s) 852
RsaI GTAC 9 cut(s) 60, 228, 240, 430, 652, 1025, 1132, 1163, 1327
RsaNI GTAC 9 cut(s) 59, 227, 239, 429, 651, 1024, 1131, 1162, 1326
RseI CAYNNNNRTG 2 cut(s) 246, 682
SacI GAGCTC 1 cut(s) 1216
SaqAI TTAA 5 cut(s) 231, 755, 1044, 1101, 1296
Sau96I GGNCC 4 cut(s) 251, 463, 537, 568
ScaI AGTACT 2 cut(s) 228, 1025
SchI GAGTC 2 cut(s) 520, 1118
ScrFI CCNGG 4 cut(s) 413, 424, 1049, 1111
SduI GDGCHC 2 cut(s) 635, 1216
SexAI ACCWGGT 1 cut(s) 1047
SfaNI GCATC 1 cut(s) 230
Sfr274I CTCGAG 1 cut(s) 311
SinI GGWCC 1 cut(s) 568
SlaI CTCGAG 1 cut(s) 311
SmiMI CAYNNNNRTG 2 cut(s) 246, 682
SmlI CTYRAG 2 cut(s) 311, 925
SmoI CTYRAG 2 cut(s) 311, 925
Sse9I AATT 7 cut(s) 72, 334, 369, 619, 1221, 1287, 1430
SseBI AGGCCT 2 cut(s) 550, 813
SspI AATATT 2 cut(s) 16, 1230
SspMI CTAG 2 cut(s) 21, 467
SstI GAGCTC 1 cut(s) 1216
StuI AGGCCT 2 cut(s) 550, 813
StyD4I CCNGG 4 cut(s) 411, 422, 1047, 1109
StyI CCWWGG 7 cut(s) 102, 130, 202, 466, 808, 844, 978
TaaI ACNGT 9 cut(s) 154, 409, 433, 460, 857, 1063, 1135, 1267, 1394
TaiI ACGT 4 cut(s) 612, 1075, 1313, 1452
TaqI TCGA 5 cut(s) 312, 761, 774, 1122, 1434
TasI AATT 7 cut(s) 72, 334, 369, 619, 1221, 1287, 1430
TatI WGTACW 4 cut(s) 58, 226, 428, 1023
TauI GCSGC 6 cut(s) 443, 798, 884, 897, 969, 1000
TfiI GAWTC 1 cut(s) 729
Tru1I TTAA 5 cut(s) 231, 755, 1044, 1101, 1296
Tru9I TTAA 5 cut(s) 231, 755, 1044, 1101, 1296
TscAI CASTG 4 cut(s) 412, 438, 1066, 1399
TseFI GTSAC 3 cut(s) 454, 1345, 1394
TseI GCWGC 6 cut(s) 189, 792, 1147, 1178, 1376, 1423
Tsp45I GTSAC 3 cut(s) 454, 1345, 1394
TspDTI ATGAA 2 cut(s) 34, 1211
TspGWI ACGGA 3 cut(s) 712, 720, 779
TspRI CASTG 4 cut(s) 412, 438, 1066, 1399
VpaK11BI GGWCC 1 cut(s) 568
XagI CCTNNNNNAGG 1 cut(s) 544
XapI RAATTY 2 cut(s) 334, 1430
XceI RCATGY 3 cut(s) 96, 687, 1242
XhoI CTCGAG 1 cut(s) 311
XmaJI CCTAGG 1 cut(s) 466
XmiI GTMKAC 2 cut(s) 690, 913
XspI CTAG 2 cut(s) 21, 467
ZrmI AGTACT 2 cut(s) 228, 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.