MD08G1051100.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000845
Physical Location & Seq
Reverse (-)
4037411 .. 4039621
2211 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1051100.v1.1.491

Sequence Viewer

Length: 1494 bp
ATGGCAACTCCCATTTCTAGCTCCTCCTCGTTCCTTAGATTTTCCATTGGTTTATCAGCATTTCTATGCCTAATTCTCTGCTCTTTGGCAGTAAGAGATACAAAAACCCACCTCCTTTCACGTACTCATACTGTTGAAGTCAACTATCTTCTACCAGCAAACACCTGCAGCCCCTCCACCAAAGATCATGACAATAAGGCGGCATCCGTACTGAAAGTGGTCCACAAGCATGGCCCATGCAACCAAGTCCACCAATCCAAATCAAACAGTAACCCTGCCGATCAAGATGAATATCTCACACAAATCCTTAACCAAGACGAAGCCCGAGTCAACTCAATCCACTCCCATTTCAACTACCCAAACAAAAAGTACAGGATCCCCGACCTACTTGCACATTCTGACGCCACAGTGATTCCCGCCCAGTCCGGCAGCGTACTTGGTTCTGGAAACTACATCGTCAGTGTCGGCTTGGGCTCTCCCCAGAAGCAACTTTCGCTTGTATTCGACACCGGCAGCAGTCTCACTTGGACACAGTGCCGACCATGTTCTGTATCTTGTTACCAACAGAAAGATCCCATCTTCGACCCTTCTCTCTCTACCTCTTACTCCAACATCTCCTGCAACTCCCCCGCGTGTTCTCAACTTGCAGCCTCCGGTGTCGCTAGCGGTTGTTCCGCCTCCACCTGTGTTTACGGCGAACAGTATGGAGACCGCTCCTTCACAATCGGCTTCTTTGCCAGCGAGAAGTTAACTTTGACTTCAACAGATGTTTTTACTGGTTTCGTCTTCGGCTGCGGCCAGAATAACCAAGGCTTATTTCGGGGCATCGCCGGTTTGCTCGGTCTCGGTCGAAGCAAGATCTCCCTTGTTGAACAAACCGCTCAGAAGTACAAGAGATTCTTCTCCTACTGCCTCCCTTCCACTTCAAGCTCCACCGGTTACCTCAGCTTAGGCCACTCCGACAACGGACGTTCAGGTTATAACGCCGTCAAGTTCGTGCCGCTCACCACATTATCTCAAGGCGAGTCGTATTACGGCCTCGGACTGGTCGGTATCAACATCGGTGGGAGTCAACTGCCAATTTCGGCTTCGGTATTTTCGACTTCGGGAACAATTATCGACTCGGGGACGGTGATCACGCGCCTCCCAGCCACTGCGTACACAGCGCTGCGGGACGCATTTCGAGAAGCGATGAAGAGGTACCCAACTGCTAGGTCGATTTCTTCCCTGCTGGACACGTGTTACGATTTCAATGGATTCAAAACGATAACCTTTCCAAAGATAGGGTTTGTGTTTGGCAATGGGGTTACGGTAGACGTGGACGCAACAGGAATATTAGTTCAGCAGAGTGCTTCGCAGTTTTGCTTGGCGTTTGCCGGAAACAAAGATGATAGGAGTGTTGGGATTATTGGGAACGTTCAACAGAAGAGGTTGCAGGTAGTGTATGACGTTGCTGGTGGAAAGGTTGGATTTGCCCCTGCTGGTTGCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

498

Amino Acids

53.03

Weight (kDa)

8.68

Isoelectric Point (pI)

36.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 150 - 204 1.1e-09 Eukaryotic aspartyl protease
TAXi_N PF14543 151 - 318 3.4e-53 Xylanase inhibitor N-terminal
Asp PF00026 234 - 494 5.5e-17 Eukaryotic aspartyl protease
TAXi_C PF14541 345 - 492 5.8e-30 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 981
AarI CACCTGC 1 cut(s) 173
Acc36I ACCTGC 2 cut(s) 173, 1426
Acc65I GGTACC 1 cut(s) 1200
AccB1I GGYRCC 1 cut(s) 1200
AccBSI CCGCTC 3 cut(s) 714, 881, 1003
AccI GTMKAC 1 cut(s) 1314
AccII CGCG 2 cut(s) 632, 1141
AclI AACGTT 1 cut(s) 1416
AclWI GGATC 3 cut(s) 370, 383, 566
AcoI YGGCCR 1 cut(s) 796
AcvI CACGTG 1 cut(s) 1239
AcyI GRCGYC 1 cut(s) 402
AfaI GTAC 7 cut(s) 124, 210, 371, 435, 890, 1160, 1202
AfeI AGCGCT 1 cut(s) 1167
AfiI CCNNNNNNNGG 2 cut(s) 1045, 1283
AflIII ACRYGT 2 cut(s) 1236, 1238
AgeI ACCGGT 1 cut(s) 935
AgsI TTSAA 8 cut(s) 137, 352, 762, 872, 927, 1252, 1261, 1421
AjiI CACGTC 1 cut(s) 1318
AjuI GAANNNNNNNTTGG 4 cut(s) 801, 833, 1072, 1104
AloI GAACNNNNNNTCC 2 cut(s) 1323, 1355
AluBI AGCT 3 cut(s) 21, 930, 948
AluI AGCT 3 cut(s) 21, 930, 948
Alw26I GTCTC 3 cut(s) 524, 702, 848
AlwI GGATC 3 cut(s) 370, 383, 566
AlwNI CAGNNNCTG 1 cut(s) 1154
Ama87I CYCGRG 2 cut(s) 324, 1123
Aor51HI AGCGCT 1 cut(s) 1167
AoxI GGCC 4 cut(s) 232, 796, 952, 1036
ApeKI GCWGC 6 cut(s) 168, 429, 513, 647, 792, 1168
ArsI GACNNNNNNTTYG 2 cut(s) 312, 344
AsiGI ACCGGT 1 cut(s) 935
Asp718I GGTACC 1 cut(s) 1200
AspLEI GCGC 2 cut(s) 1143, 1168
AspS9I GGNCC 2 cut(s) 220, 233
AsuHPI GGTGA 2 cut(s) 997, 1144
AsuNHI GCTAGC 1 cut(s) 662
AvaI CYCGRG 2 cut(s) 324, 1123
AvaII GGWCC 1 cut(s) 220
BamHI GGATCC 1 cut(s) 375
BanI GGYRCC 1 cut(s) 1200
BanII GRGCYC 1 cut(s) 476
BbrPI CACGTG 1 cut(s) 1239
BbsI GAAGAC 1 cut(s) 778
BbvCI CCTCAGC 1 cut(s) 944
BbvI GCAGC 6 cut(s) 180, 441, 525, 659, 779, 1155
BccI CCATC 1 cut(s) 584
BceAI ACGGC 3 cut(s) 709, 971, 1051
BcgI CGANNNNNNTGC 2 cut(s) 371, 405
BclI TGATCA 1 cut(s) 1134
BcoDI GTCTC 3 cut(s) 524, 702, 848
BfaI CTAG 3 cut(s) 18, 663, 1212
BfmI CTRYAG 1 cut(s) 166
BfoI RGCGCY 1 cut(s) 1169
BfuAI ACCTGC 2 cut(s) 173, 1426
BglII AGATCT 1 cut(s) 858
BisI GCNGC 9 cut(s) 169, 201, 430, 514, 648, 793, 796, 1001, 1169
BlsI GCNGC 9 cut(s) 170, 202, 431, 515, 649, 794, 797, 1002, 1170
Bme18I GGWCC 1 cut(s) 220
BmeT110I CYCGRG 2 cut(s) 324, 1123
BmgBI CACGTC 1 cut(s) 1318
BmgT120I GGNCC 2 cut(s) 220, 233
BmiI GGNNCC 2 cut(s) 377, 1202
BmrI ACTGGG 1 cut(s) 415
BmsI GCATC 2 cut(s) 212, 834
BmtI GCTAGC 1 cut(s) 666
BmuI ACTGGG 1 cut(s) 415
BpiI GAAGAC 1 cut(s) 778
Bpu10I CCTNAGC 2 cut(s) 944, 949
BpuEI CTTGAG 1 cut(s) 1002
BsaAI YACGTR 2 cut(s) 122, 1239
BsaBI GATNNNNATC 1 cut(s) 291
BsaHI GRCGYC 1 cut(s) 402
BsaI GGTCTC 2 cut(s) 702, 848
BsaJI CCNNGG 2 cut(s) 808, 1039
BsaWI WCCGGW 2 cut(s) 653, 935
Bsc4I CCNNNNNNNGG 2 cut(s) 1045, 1283
Bse118I RCCGGY 3 cut(s) 509, 830, 935
Bse1I ACTGG 3 cut(s) 421, 781, 1050
Bse3DI GCAATG 1 cut(s) 1306
Bse8I GATNNNNATC 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 808, 1039
BseGI GGATG 1 cut(s) 203
BseJI GATNNNNATC 1 cut(s) 291
BseLI CCNNNNNNNGG 2 cut(s) 1045, 1283
BseMI GCAATG 1 cut(s) 1306
BseMII CTCAG 2 cut(s) 896, 958
BseNI ACTGG 3 cut(s) 421, 781, 1050
BseRI GAGGAG 2 cut(s) 13, 16
BseXI GCAGC 6 cut(s) 180, 441, 525, 659, 779, 1155
BseYI CCCAGC 1 cut(s) 1147
Bsh1236I CGCG 2 cut(s) 632, 1141
Bsh1285I CGRYCG 1 cut(s) 850
BshFI GGCC 4 cut(s) 234, 798, 954, 1038
BshNI GGYRCC 1 cut(s) 1200
BshTI ACCGGT 1 cut(s) 935
BsiEI CGRYCG 1 cut(s) 850
BsiHKCI CYCGRG 2 cut(s) 324, 1123
BsiSI CCGG 6 cut(s) 426, 510, 654, 831, 936, 1377
BslFI GGGAC 2 cut(s) 1141, 1187
BslI CCNNNNNNNGG 2 cut(s) 1045, 1283
BsmAI GTCTC 3 cut(s) 524, 702, 848
BsmFI GGGAC 2 cut(s) 1141, 1187
BsnI GGCC 4 cut(s) 234, 798, 954, 1038
Bso31I GGTCTC 2 cut(s) 702, 848
BsoBI CYCGRG 2 cut(s) 324, 1123
Bsp1286I GDGCHC 1 cut(s) 476
Bsp143I GATC 6 cut(s) 184, 280, 375, 571, 858, 1134
BspANI GGCC 4 cut(s) 234, 798, 954, 1038
BspCNI CTCAG 2 cut(s) 895, 957
BspFNI CGCG 2 cut(s) 632, 1141
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 2 cut(s) 377, 1202
BspMAI CTGCAG 1 cut(s) 170
BspMI ACCTGC 2 cut(s) 173, 1426
BspOI GCTAGC 1 cut(s) 666
BspPI GGATC 3 cut(s) 370, 383, 566
BspT107I GGYRCC 1 cut(s) 1200
BspTNI GGTCTC 2 cut(s) 702, 848
BsrBI CCGCTC 3 cut(s) 714, 881, 1003
BsrDI GCAATG 1 cut(s) 1306
BsrFI RCCGGY 3 cut(s) 509, 830, 935
BsrI ACTGG 3 cut(s) 421, 781, 1050
BssAI RCCGGY 3 cut(s) 509, 830, 935
BssECI CCNNGG 2 cut(s) 808, 1039
BssMI GATC 6 cut(s) 184, 280, 375, 571, 858, 1134
BssNI GRCGYC 1 cut(s) 402
BssT1I CCWWGG 1 cut(s) 808
Bst4CI ACNGT 7 cut(s) 133, 269, 409, 534, 702, 1132, 1312
Bst6I CTCTTC 2 cut(s) 1190, 1421
BstACI GRCGYC 1 cut(s) 402
BstBAI YACGTR 2 cut(s) 122, 1239
BstC8I GCNNGC 2 cut(s) 664, 739
BstDEI CTNAG 4 cut(s) 35, 882, 944, 949
BstEII GGTNACC 1 cut(s) 938
BstF5I GGATG 1 cut(s) 203
BstFNI CGCG 2 cut(s) 632, 1141
BstH2I RGCGCY 1 cut(s) 1169
BstHHI GCGC 2 cut(s) 1143, 1168
BstKTI GATC 6 cut(s) 187, 283, 378, 574, 861, 1137
BstMAI GTCTC 3 cut(s) 524, 702, 848
BstMBI GATC 6 cut(s) 184, 280, 375, 571, 858, 1134
BstMCI CGRYCG 1 cut(s) 850
BstMWI GCNNNNNNNGC 2 cut(s) 493, 1163
BstPI GGTNACC 1 cut(s) 938
BstSFI CTRYAG 1 cut(s) 166
BstUI CGCG 2 cut(s) 632, 1141
BstV1I GCAGC 6 cut(s) 180, 441, 525, 659, 779, 1155
BstV2I GAAGAC 1 cut(s) 778
BstX2I RGATCY 3 cut(s) 375, 571, 858
BstXI CCANNNNNNTGG 1 cut(s) 230
BstYI RGATCY 3 cut(s) 375, 571, 858
BsuRI GGCC 4 cut(s) 234, 798, 954, 1038
BtgZI GCGATG 2 cut(s) 811, 1205
BtrI CACGTC 1 cut(s) 1318
BtsCI GGATG 1 cut(s) 203
BtsI GCAGTG 1 cut(s) 1152
BtsIMutI CAGTG 4 cut(s) 414, 466, 539, 1152
BveI ACCTGC 2 cut(s) 173, 1426
Cac8I GCNNGC 2 cut(s) 664, 739
CaiI CAGNNNCTG 1 cut(s) 1154
CciI TCATGA 1 cut(s) 187
CfoI GCGC 2 cut(s) 1143, 1168
Cfr10I RCCGGY 3 cut(s) 509, 830, 935
Cfr13I GGNCC 2 cut(s) 220, 233
CseI GACGC 3 cut(s) 410, 1184, 1331
Csp6I GTAC 7 cut(s) 123, 209, 370, 434, 889, 1159, 1201
CspAI ACCGGT 1 cut(s) 935
CspCI CAANNNNNGTGG 2 cut(s) 1045, 1080
CviAII CATG 4 cut(s) 188, 230, 237, 543
CviQI GTAC 7 cut(s) 123, 209, 370, 434, 889, 1159, 1201
DdeI CTNAG 4 cut(s) 35, 882, 944, 949
DpnI GATC 6 cut(s) 186, 282, 377, 573, 860, 1136
DpnII GATC 6 cut(s) 184, 280, 375, 571, 858, 1134
EaeI YGGCCR 1 cut(s) 796
Eam1104I CTCTTC 2 cut(s) 1190, 1421
EarI CTCTTC 2 cut(s) 1190, 1421
EciI GGCGGA 1 cut(s) 664
Eco130I CCWWGG 1 cut(s) 808
Eco24I GRGCYC 1 cut(s) 476
Eco31I GGTCTC 2 cut(s) 702, 848
Eco47I GGWCC 1 cut(s) 220
Eco47III AGCGCT 1 cut(s) 1167
Eco72I CACGTG 1 cut(s) 1239
Eco88I CYCGRG 2 cut(s) 324, 1123
Eco91I GGTNACC 1 cut(s) 938
EcoO65I GGTNACC 1 cut(s) 938
EcoT14I CCWWGG 1 cut(s) 808
EcoT38I GRGCYC 1 cut(s) 476
ErhI CCWWGG 1 cut(s) 808
FaeI CATG 4 cut(s) 191, 233, 240, 546
FaiI YATR 9 cut(s) 67, 129, 189, 231, 238, 544, 705, 981, 1446
FalI AAGNNNNNCTT 2 cut(s) 884, 916
FaqI GGGAC 2 cut(s) 1141, 1187
FatI CATG 4 cut(s) 187, 229, 236, 542
FauI CCCGC 3 cut(s) 424, 637, 1164
FbaI TGATCA 1 cut(s) 1134
FblI GTMKAC 1 cut(s) 1314
Fnu4HI GCNGC 9 cut(s) 169, 201, 430, 514, 648, 793, 796, 1001, 1169
FokI GGATG 1 cut(s) 190
FriOI GRGCYC 1 cut(s) 476
Fsp4HI GCNGC 9 cut(s) 169, 201, 430, 514, 648, 793, 796, 1001, 1169
FspBI CTAG 3 cut(s) 18, 663, 1212
GlaI GCGC 2 cut(s) 1142, 1167
GluI GCNGC 9 cut(s) 169, 201, 430, 514, 648, 793, 796, 1001, 1169
GsaI CCCAGC 1 cut(s) 1151
HaeII RGCGCY 1 cut(s) 1169
HaeIII GGCC 4 cut(s) 234, 798, 954, 1038
HapII CCGG 6 cut(s) 426, 510, 654, 831, 936, 1377
HgaI GACGC 3 cut(s) 410, 1184, 1331
HhaI GCGC 2 cut(s) 1143, 1168
Hin1I GRCGYC 1 cut(s) 402
Hin1II CATG 4 cut(s) 191, 233, 240, 546
Hin6I GCGC 2 cut(s) 1141, 1166
HinP1I GCGC 2 cut(s) 1141, 1166
HincII GTYRAC 4 cut(s) 142, 331, 750, 1073
HindII GTYRAC 4 cut(s) 142, 331, 750, 1073
HinfI GANTC 7 cut(s) 327, 412, 897, 1025, 1069, 1121, 1257
HpaI GTTAAC 1 cut(s) 750
HpaII CCGG 6 cut(s) 426, 510, 654, 831, 936, 1377
HphI GGTGA 2 cut(s) 997, 1144
Hpy188I TCNGA 4 cut(s) 400, 885, 961, 1043
Hpy188III TCNNGA 5 cut(s) 188, 284, 444, 1107, 1184
HpyAV CCTTC 3 cut(s) 597, 727, 927
HpyCH4III ACNGT 7 cut(s) 133, 269, 409, 534, 702, 1132, 1312
HpyCH4IV ACGT 6 cut(s) 121, 970, 1238, 1317, 1416, 1449
HpyCH4V TGCA 6 cut(s) 168, 240, 392, 621, 647, 1435
HpyF10VI GCNNNNNNNGC 2 cut(s) 493, 1163
HpyF3I CTNAG 4 cut(s) 35, 882, 944, 949
HpySE526I ACGT 6 cut(s) 121, 970, 1238, 1317, 1416, 1449
Hsp92I GRCGYC 1 cut(s) 402
Hsp92II CATG 4 cut(s) 191, 233, 240, 546
HspAI GCGC 2 cut(s) 1141, 1166
KpnI GGTACC 1 cut(s) 1204
Ksp22I TGATCA 1 cut(s) 1134
KspAI GTTAAC 1 cut(s) 750
Kzo9I GATC 6 cut(s) 184, 280, 375, 571, 858, 1134
LmnI GCTCC 3 cut(s) 26, 719, 935
Lsp1109I GCAGC 6 cut(s) 180, 441, 525, 659, 779, 1155
LweI GCATC 2 cut(s) 212, 834
MaeI CTAG 3 cut(s) 18, 663, 1212
MaeII ACGT 6 cut(s) 121, 970, 1238, 1317, 1416, 1449
MaeIII GTNAC 5 cut(s) 269, 557, 938, 1241, 1306
MalI GATC 6 cut(s) 186, 282, 377, 573, 860, 1136
MbiI CCGCTC 3 cut(s) 714, 881, 1003
MboI GATC 6 cut(s) 184, 280, 375, 571, 858, 1134
MboII GAAGA 7 cut(s) 140, 571, 778, 892, 1207, 1215, 1438
MflI RGATCY 3 cut(s) 375, 571, 858
MhlI GDGCHC 1 cut(s) 476
MluCI AATT 3 cut(s) 72, 1080, 1113
MlyI GAGTC 4 cut(s) 336, 1034, 1078, 1115
MmeI TCCRAC 3 cut(s) 633, 984, 1447
MseI TTAA 2 cut(s) 309, 749
MslI CAYNNNNRTG 2 cut(s) 64, 228
MspI CCGG 6 cut(s) 426, 510, 654, 831, 936, 1377
MvnI CGCG 2 cut(s) 632, 1141
MwoI GCNNNNNNNGC 2 cut(s) 493, 1163
NdeII GATC 6 cut(s) 184, 280, 375, 571, 858, 1134
NheI GCTAGC 1 cut(s) 662
NlaIII CATG 4 cut(s) 191, 233, 240, 546
NlaIV GGNNCC 2 cut(s) 377, 1202
PagI TCATGA 1 cut(s) 187
PaqCI CACCTGC 1 cut(s) 173
PcsI WCGNNNNNNNCGW 1 cut(s) 1097
PfeI GAWTC 3 cut(s) 412, 897, 1257
PinAI ACCGGT 1 cut(s) 935
PkrI GCNGC 9 cut(s) 170, 202, 431, 515, 649, 794, 797, 1002, 1170
PleI GAGTC 4 cut(s) 335, 1033, 1077, 1115
PmaCI CACGTG 1 cut(s) 1239
PmlI CACGTG 1 cut(s) 1239
PpsI GAGTC 4 cut(s) 335, 1033, 1077, 1115
Ppu21I YACGTR 2 cut(s) 122, 1239
PsiI TTATAA 1 cut(s) 981
Psp1406I AACGTT 1 cut(s) 1416
PspCI CACGTG 1 cut(s) 1239
PspEI GGTNACC 1 cut(s) 938
PspFI CCCAGC 1 cut(s) 1147
PspN4I GGNNCC 2 cut(s) 377, 1202
PspPI GGNCC 2 cut(s) 220, 233
PstI CTGCAG 1 cut(s) 170
PstNI CAGNNNCTG 1 cut(s) 1154
PsuI RGATCY 3 cut(s) 375, 571, 858
RsaI GTAC 7 cut(s) 124, 210, 371, 435, 890, 1160, 1202
RsaNI GTAC 7 cut(s) 123, 209, 370, 434, 889, 1159, 1201
RseI CAYNNNNRTG 2 cut(s) 64, 228
SaqAI TTAA 2 cut(s) 309, 749
SatI GCNGC 9 cut(s) 169, 201, 430, 514, 648, 793, 796, 1001, 1169
Sau3AI GATC 6 cut(s) 184, 280, 375, 571, 858, 1134
Sau96I GGNCC 2 cut(s) 220, 233
SchI GAGTC 4 cut(s) 336, 1034, 1078, 1115
SduI GDGCHC 1 cut(s) 476
SfaNI GCATC 2 cut(s) 212, 834
SfcI CTRYAG 1 cut(s) 166
SinI GGWCC 1 cut(s) 220
SmiMI CAYNNNNRTG 2 cut(s) 64, 228
SmlI CTYRAG 1 cut(s) 1017
SmoI CTYRAG 1 cut(s) 1017
Sse9I AATT 3 cut(s) 72, 1080, 1113
SspI AATATT 1 cut(s) 1335
SspMI CTAG 3 cut(s) 18, 663, 1212
StyI CCWWGG 1 cut(s) 808
TaaI ACNGT 7 cut(s) 133, 269, 409, 534, 702, 1132, 1312
TaiI ACGT 6 cut(s) 124, 973, 1241, 1320, 1419, 1452
TaqI TCGA 7 cut(s) 504, 582, 850, 1100, 1119, 1183, 1217
TaqII GACCGA 2 cut(s) 830, 836
TasI AATT 3 cut(s) 72, 1080, 1113
TatI WGTACW 2 cut(s) 369, 888
TauI GCSGC 3 cut(s) 203, 798, 1003
TfiI GAWTC 3 cut(s) 412, 897, 1257
Tru1I TTAA 2 cut(s) 309, 749
Tru9I TTAA 2 cut(s) 309, 749
TscAI CASTG 4 cut(s) 414, 466, 539, 1159
TseI GCWGC 6 cut(s) 168, 429, 513, 647, 792, 1168
TspDTI ATGAA 2 cut(s) 303, 1208
TspGWI ACGGA 2 cut(s) 196, 981
TspRI CASTG 4 cut(s) 414, 466, 539, 1159
VpaK11BI GGWCC 1 cut(s) 220
XmiI GTMKAC 1 cut(s) 1314
XspI CTAG 3 cut(s) 18, 663, 1212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.