MD08G1050700.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000845
Physical Location & Seq
Reverse (-)
3970113 .. 3972375
2263 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1050700.v1.1.491

Sequence Viewer

Length: 1482 bp
ATGGCAACTCCCATTTCTAGCTCCTCCTCCTCCCTTAGATTTTCCATTGGTTTATCAGCATTTCTATGCCTAATTCTCTGTTCTTTGGCAGTAAGAGATACAAAAACCAACCTCCTTTCACGTACTTACACAGTTGAAGTCATCTCTCTTCTACCAGCAAACACCTGCAGACCCTCCACCAATGGTCATGACGCATCCGTACTGAAGGTGGTCCACAAATACGGCCCATGCAACCAAGTCCACCAAGTCAAATCAAACACTAACCCTTCCGATCAAGATGAATATCACATACAAATCCTCAAGCAAGACGAAGCCCGAGTCAACTCAATCCACTCCCATTTCAGCGACTCAAACCGTAATTACAGAATCCCCGACCCACTTGCACAATCAGACGCCACCGTGATTCCCGCCCAGTCTGCCAGCGTACTTGGTTCCGGAAACTACATCGTCAGTGTCGGCTTGGGCTCGCCCCAGAAGCAACTCTCGCTTGTATTCGACACCGGCAGCAGTCTCACTTGGACACAGTGCCGACCATGTTCTGTATCTTGTTACAAACAGAAAGATCCCATCTTCGACCCTTCTCTCTCTACCTCTTACTCCAACATCTCCTGCAACTCCCCCGCGTGTTCTCAACTTGCAGCCTCCGGTGTCGCTAGCGGTTGTTCCGCCTCCACCTGTGTATACGGCCAACAGTATGGAGACCGCTCCTTCACAATCGGCTTCTTTGCCAGCGAGAAGTTAACTTTGACTTCAACAGATGTTTTTACTGGTTTCGTCTTCGGCTGCGGCCAGAATAACCAAGGCTTATTTAGGGGCATCGCCGGTTTGCTCGGTCTCGGTCGAAGCAAGATCTCCCTTGTTGAACAAACCGCTCAGAAGTACAAGAGATTCTTCTCCTACTGCCTCCCTTCCACTTCAAGCTCCACTGGTTACCTCAGTTTAGGTAACTCCGACAACAGAAGTTCAGGTAACGCCGTCAAGTTCACGCCGCTCACCACATTATCTCAAGGCGCGTCGTATTACAGCCTCGGACTGGTCGGCATCAACATCGGTGGGAGTCAACTGCCAATTTCGGCTTCAGTATTTTCGATTTCGGGGACAATCATCGACTCGGGGACGGTCATCACTCGCCTCCCAGCCACAGCGTACACAGCGCTGCGGGACGCATTTCGAGAAGTGATGAAGAGATACCCAACTGCTAAGTCGATTTCTTCCCTGCTGGACACGTGTTACGACTTCAGTGGATTCAAAACGATAACCTTTCCAACGATAGGGTTTGTGTTTGGCAATGGGGTTACGGTAGACGTGGACGCAACAGGAATATTATTCCAGCAGAGTGCTTCGCAGTTTTGCTTGGCGTTTGCCGGAAACAAAGATGATAGTAGTGTTGGGATTATTGGGAACGTTCAACAGAAGAGGTTGCAGGTAGTGTATGACGTTGCTGGTGGAAAGGTTGGATTTGCACCTGCTGGTTGCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

494

Amino Acids

52.37

Weight (kDa)

8.69

Isoelectric Point (pI)

36.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 147 - 201 2.2e-09 Eukaryotic aspartyl protease
TAXi_N PF14543 148 - 316 1.1e-53 Xylanase inhibitor N-terminal
Asp PF00026 231 - 490 1.2e-17 Eukaryotic aspartyl protease
TAXi_C PF14541 341 - 488 2.6e-29 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 173, 1474
Acc36I ACCTGC 3 cut(s) 173, 1414, 1474
AccBSI CCGCTC 3 cut(s) 705, 872, 991
AccI GTMKAC 2 cut(s) 681, 1302
AccII CGCG 2 cut(s) 623, 1013
AccIII TCCGGA 1 cut(s) 434
AciI CCGC 9 cut(s) 408, 621, 657, 666, 703, 786, 870, 989, 1159
AclI AACGTT 1 cut(s) 1404
AclWI GGATC 1 cut(s) 557
AcoI YGGCCR 2 cut(s) 685, 787
AcuI CTGAAG 3 cut(s) 224, 1062, 1222
AcvI CACGTG 1 cut(s) 1227
AcyI GRCGYC 1 cut(s) 393
AfaI GTAC 5 cut(s) 124, 201, 426, 881, 1148
AfeI AGCGCT 1 cut(s) 1155
AfiI CCNNNNNNNGG 2 cut(s) 1033, 1271
AflIII ACRYGT 2 cut(s) 1224, 1226
AgsI TTSAA 6 cut(s) 137, 753, 863, 918, 1249, 1409
AjiI CACGTC 1 cut(s) 1306
AjuI GAANNNNNNNTTGG 2 cut(s) 1060, 1092
AluBI AGCT 2 cut(s) 21, 921
AluI AGCT 2 cut(s) 21, 921
Alw26I GTCTC 3 cut(s) 515, 693, 839
AlwI GGATC 1 cut(s) 557
Ama87I CYCGRG 2 cut(s) 315, 1111
Aor13HI TCCGGA 1 cut(s) 434
Aor51HI AGCGCT 1 cut(s) 1155
AoxI GGCC 3 cut(s) 223, 685, 787
ApeKI GCWGC 4 cut(s) 504, 638, 783, 1156
ArsI GACNNNNNNTTYG 2 cut(s) 303, 335
AspLEI GCGC 2 cut(s) 1013, 1156
AspS9I GGNCC 2 cut(s) 211, 224
AsuHPI GGTGA 1 cut(s) 985
AsuNHI GCTAGC 1 cut(s) 653
AvaI CYCGRG 2 cut(s) 315, 1111
AvaII GGWCC 1 cut(s) 211
BanII GRGCYC 1 cut(s) 467
BbrPI CACGTG 1 cut(s) 1227
BbsI GAAGAC 1 cut(s) 769
BbvI GCAGC 4 cut(s) 516, 650, 770, 1143
BccI CCATC 1 cut(s) 575
BceAI ACGGC 3 cut(s) 238, 700, 959
BcgI CGANNNNNNTGC 4 cut(s) 362, 396, 1030, 1064
BcoDI GTCTC 3 cut(s) 515, 693, 839
BfaI CTAG 2 cut(s) 18, 654
BfmI CTRYAG 1 cut(s) 166
BfoI RGCGCY 1 cut(s) 1157
BfuAI ACCTGC 3 cut(s) 173, 1414, 1474
BglII AGATCT 1 cut(s) 849
BisI GCNGC 6 cut(s) 505, 639, 784, 787, 989, 1157
BlsI GCNGC 6 cut(s) 506, 640, 785, 788, 990, 1158
Bme18I GGWCC 1 cut(s) 211
BmeT110I CYCGRG 2 cut(s) 315, 1111
BmgBI CACGTC 1 cut(s) 1306
BmgT120I GGNCC 2 cut(s) 211, 224
BmiI GGNNCC 1 cut(s) 433
BmrI ACTGGG 1 cut(s) 406
BmsI GCATC 3 cut(s) 203, 825, 1050
BmtI GCTAGC 1 cut(s) 657
BmuI ACTGGG 1 cut(s) 406
BpiI GAAGAC 1 cut(s) 769
BpuEI CTTGAG 2 cut(s) 284, 990
BsaAI YACGTR 2 cut(s) 122, 1227
BsaBI GATNNNNATC 1 cut(s) 282
BsaHI GRCGYC 1 cut(s) 393
BsaI GGTCTC 2 cut(s) 693, 839
BsaJI CCNNGG 2 cut(s) 799, 1027
BsaWI WCCGGW 2 cut(s) 434, 644
Bsc4I CCNNNNNNNGG 2 cut(s) 1033, 1271
Bse118I RCCGGY 2 cut(s) 500, 821
Bse1I ACTGG 4 cut(s) 412, 772, 931, 1038
Bse3DI GCAATG 1 cut(s) 1294
Bse8I GATNNNNATC 1 cut(s) 282
BseAI TCCGGA 1 cut(s) 434
BseDI CCNNGG 2 cut(s) 799, 1027
BseGI GGATG 1 cut(s) 194
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 2 cut(s) 1033, 1271
BseMI GCAATG 1 cut(s) 1294
BseMII CTCAG 2 cut(s) 887, 949
BseNI ACTGG 4 cut(s) 412, 772, 931, 1038
BseRI GAGGAG 3 cut(s) 13, 16, 19
BseXI GCAGC 4 cut(s) 516, 650, 770, 1143
BseYI CCCAGC 1 cut(s) 1135
Bsh1236I CGCG 2 cut(s) 623, 1013
Bsh1285I CGRYCG 1 cut(s) 841
BshFI GGCC 3 cut(s) 225, 687, 789
BsiEI CGRYCG 1 cut(s) 841
BsiHKCI CYCGRG 2 cut(s) 315, 1111
BsiSI CCGG 5 cut(s) 435, 501, 645, 822, 1365
BslFI GGGAC 3 cut(s) 1111, 1129, 1175
BslI CCNNNNNNNGG 2 cut(s) 1033, 1271
BsmAI GTCTC 3 cut(s) 515, 693, 839
BsmFI GGGAC 3 cut(s) 1111, 1129, 1175
BsnI GGCC 3 cut(s) 225, 687, 789
Bso31I GGTCTC 2 cut(s) 693, 839
BsoBI CYCGRG 2 cut(s) 315, 1111
Bsp1286I GDGCHC 1 cut(s) 467
Bsp13I TCCGGA 1 cut(s) 434
Bsp143I GATC 3 cut(s) 271, 562, 849
BspACI CCGC 9 cut(s) 408, 621, 657, 666, 703, 786, 870, 989, 1159
BspANI GGCC 3 cut(s) 225, 687, 789
BspCNI CTCAG 2 cut(s) 886, 948
BspEI TCCGGA 1 cut(s) 434
BspFNI CGCG 2 cut(s) 623, 1013
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 1 cut(s) 433
BspMAI CTGCAG 1 cut(s) 170
BspMI ACCTGC 3 cut(s) 173, 1414, 1474
BspOI GCTAGC 1 cut(s) 657
BspPI GGATC 1 cut(s) 557
BspTNI GGTCTC 2 cut(s) 693, 839
BsrBI CCGCTC 3 cut(s) 705, 872, 991
BsrDI GCAATG 1 cut(s) 1294
BsrFI RCCGGY 2 cut(s) 500, 821
BsrI ACTGG 4 cut(s) 412, 772, 931, 1038
BssAI RCCGGY 2 cut(s) 500, 821
BssECI CCNNGG 2 cut(s) 799, 1027
BssMI GATC 3 cut(s) 271, 562, 849
BssNAI GTATAC 1 cut(s) 682
BssNI GRCGYC 1 cut(s) 393
BssT1I CCWWGG 1 cut(s) 799
Bst1107I GTATAC 1 cut(s) 682
Bst4CI ACNGT 7 cut(s) 133, 356, 400, 525, 693, 1120, 1300
Bst6I CTCTTC 3 cut(s) 153, 1178, 1409
BstACI GRCGYC 1 cut(s) 393
BstBAI YACGTR 2 cut(s) 122, 1227
BstC8I GCNNGC 4 cut(s) 421, 467, 655, 730
BstDEI CTNAG 4 cut(s) 35, 873, 935, 1200
BstEII GGTNACC 1 cut(s) 929
BstF5I GGATG 1 cut(s) 194
BstFNI CGCG 2 cut(s) 623, 1013
BstH2I RGCGCY 1 cut(s) 1157
BstHHI GCGC 2 cut(s) 1013, 1156
BstKTI GATC 3 cut(s) 274, 565, 852
BstMAI GTCTC 3 cut(s) 515, 693, 839
BstMBI GATC 3 cut(s) 271, 562, 849
BstMCI CGRYCG 1 cut(s) 841
BstMWI GCNNNNNNNGC 4 cut(s) 416, 475, 484, 1151
BstPI GGTNACC 1 cut(s) 929
BstSFI CTRYAG 1 cut(s) 166
BstUI CGCG 2 cut(s) 623, 1013
BstV1I GCAGC 4 cut(s) 516, 650, 770, 1143
BstV2I GAAGAC 1 cut(s) 769
BstX2I RGATCY 2 cut(s) 562, 849
BstXI CCANNNNNNTGG 1 cut(s) 695
BstYI RGATCY 2 cut(s) 562, 849
BstZ17I GTATAC 1 cut(s) 682
BsuRI GGCC 3 cut(s) 225, 687, 789
BtgZI GCGATG 1 cut(s) 802
BtrI CACGTC 1 cut(s) 1306
BtsCI GGATG 1 cut(s) 194
BtsIMutI CAGTG 4 cut(s) 457, 530, 924, 1246
BveI ACCTGC 3 cut(s) 173, 1414, 1474
Cac8I GCNNGC 4 cut(s) 421, 467, 655, 730
CciI TCATGA 1 cut(s) 187
CfoI GCGC 2 cut(s) 1013, 1156
Cfr10I RCCGGY 2 cut(s) 500, 821
Cfr13I GGNCC 2 cut(s) 211, 224
CseI GACGC 5 cut(s) 200, 401, 1002, 1172, 1319
Csp6I GTAC 5 cut(s) 123, 200, 425, 880, 1147
CspCI CAANNNNNGTGG 2 cut(s) 1033, 1068
CviAII CATG 3 cut(s) 188, 228, 534
CviQI GTAC 5 cut(s) 123, 200, 425, 880, 1147
DdeI CTNAG 4 cut(s) 35, 873, 935, 1200
DpnI GATC 3 cut(s) 273, 564, 851
DpnII GATC 3 cut(s) 271, 562, 849
EaeI YGGCCR 2 cut(s) 685, 787
Eam1104I CTCTTC 3 cut(s) 153, 1178, 1409
EarI CTCTTC 3 cut(s) 153, 1178, 1409
EciI GGCGGA 1 cut(s) 655
Eco130I CCWWGG 1 cut(s) 799
Eco24I GRGCYC 1 cut(s) 467
Eco31I GGTCTC 2 cut(s) 693, 839
Eco47I GGWCC 1 cut(s) 211
Eco47III AGCGCT 1 cut(s) 1155
Eco57I CTGAAG 3 cut(s) 224, 1062, 1222
Eco72I CACGTG 1 cut(s) 1227
Eco88I CYCGRG 2 cut(s) 315, 1111
Eco91I GGTNACC 1 cut(s) 929
EcoO65I GGTNACC 1 cut(s) 929
EcoT14I CCWWGG 1 cut(s) 799
EcoT38I GRGCYC 1 cut(s) 467
ErhI CCWWGG 1 cut(s) 799
FaeI CATG 3 cut(s) 191, 231, 537
FaiI YATR 8 cut(s) 67, 189, 229, 290, 535, 682, 696, 1434
FalI AAGNNNNNCTT 2 cut(s) 875, 907
FaqI GGGAC 3 cut(s) 1111, 1129, 1175
FatI CATG 3 cut(s) 187, 227, 533
FauI CCCGC 3 cut(s) 415, 628, 1152
FblI GTMKAC 2 cut(s) 681, 1302
Fnu4HI GCNGC 6 cut(s) 505, 639, 784, 787, 989, 1157
FokI GGATG 1 cut(s) 181
FriOI GRGCYC 1 cut(s) 467
Fsp4HI GCNGC 6 cut(s) 505, 639, 784, 787, 989, 1157
FspBI CTAG 2 cut(s) 18, 654
GlaI GCGC 2 cut(s) 1012, 1155
GluI GCNGC 6 cut(s) 505, 639, 784, 787, 989, 1157
GsaI CCCAGC 1 cut(s) 1139
HaeII RGCGCY 1 cut(s) 1157
HaeIII GGCC 3 cut(s) 225, 687, 789
HapII CCGG 5 cut(s) 435, 501, 645, 822, 1365
HgaI GACGC 5 cut(s) 200, 401, 1002, 1172, 1319
HhaI GCGC 2 cut(s) 1013, 1156
Hin1I GRCGYC 1 cut(s) 393
Hin1II CATG 3 cut(s) 191, 231, 537
Hin6I GCGC 2 cut(s) 1011, 1154
HinP1I GCGC 2 cut(s) 1011, 1154
HincII GTYRAC 3 cut(s) 322, 741, 1061
HindII GTYRAC 3 cut(s) 322, 741, 1061
HinfI GANTC 8 cut(s) 318, 347, 366, 403, 888, 1057, 1109, 1245
HpaI GTTAAC 1 cut(s) 741
HpaII CCGG 5 cut(s) 435, 501, 645, 822, 1365
HphI GGTGA 1 cut(s) 985
Hpy188I TCNGA 5 cut(s) 271, 391, 876, 952, 1031
Hpy188III TCNNGA 4 cut(s) 188, 275, 435, 1172
Hpy99I CGWCG 1 cut(s) 1018
HpyAV CCTTC 5 cut(s) 199, 276, 588, 718, 918
HpyCH4III ACNGT 7 cut(s) 133, 356, 400, 525, 693, 1120, 1300
HpyCH4IV ACGT 5 cut(s) 121, 1226, 1305, 1404, 1437
HpyCH4V TGCA 7 cut(s) 168, 231, 383, 612, 638, 1423, 1463
HpyF10VI GCNNNNNNNGC 4 cut(s) 416, 475, 484, 1151
HpyF3I CTNAG 4 cut(s) 35, 873, 935, 1200
HpySE526I ACGT 5 cut(s) 121, 1226, 1305, 1404, 1437
Hsp92I GRCGYC 1 cut(s) 393
Hsp92II CATG 3 cut(s) 191, 231, 537
HspAI GCGC 2 cut(s) 1011, 1154
Kpn2I TCCGGA 1 cut(s) 434
KspAI GTTAAC 1 cut(s) 741
Kzo9I GATC 3 cut(s) 271, 562, 849
LmnI GCTCC 3 cut(s) 26, 710, 926
Lsp1109I GCAGC 4 cut(s) 516, 650, 770, 1143
LweI GCATC 3 cut(s) 203, 825, 1050
MaeI CTAG 2 cut(s) 18, 654
MaeII ACGT 5 cut(s) 121, 1226, 1305, 1404, 1437
MaeIII GTNAC 6 cut(s) 548, 929, 944, 968, 1229, 1294
MalI GATC 3 cut(s) 273, 564, 851
MbiI CCGCTC 3 cut(s) 705, 872, 991
MboI GATC 3 cut(s) 271, 562, 849
MboII GAAGA 7 cut(s) 140, 562, 769, 883, 1195, 1203, 1426
MflI RGATCY 2 cut(s) 562, 849
MhlI GDGCHC 1 cut(s) 467
MluCI AATT 3 cut(s) 72, 358, 1068
MlyI GAGTC 4 cut(s) 327, 341, 1066, 1103
MmeI TCCRAC 4 cut(s) 624, 975, 1289, 1435
MroI TCCGGA 1 cut(s) 434
MseI TTAA 1 cut(s) 740
MslI CAYNNNNRTG 1 cut(s) 64
MspI CCGG 5 cut(s) 435, 501, 645, 822, 1365
MvnI CGCG 2 cut(s) 623, 1013
MwoI GCNNNNNNNGC 4 cut(s) 416, 475, 484, 1151
NdeII GATC 3 cut(s) 271, 562, 849
NheI GCTAGC 1 cut(s) 653
NlaIII CATG 3 cut(s) 191, 231, 537
NlaIV GGNNCC 1 cut(s) 433
PagI TCATGA 1 cut(s) 187
PaqCI CACCTGC 2 cut(s) 173, 1474
PfeI GAWTC 4 cut(s) 366, 403, 888, 1245
PkrI GCNGC 6 cut(s) 506, 640, 785, 788, 990, 1158
PleI GAGTC 4 cut(s) 326, 341, 1065, 1103
PmaCI CACGTG 1 cut(s) 1227
PmlI CACGTG 1 cut(s) 1227
PpsI GAGTC 4 cut(s) 326, 341, 1065, 1103
Ppu21I YACGTR 2 cut(s) 122, 1227
Psp1406I AACGTT 1 cut(s) 1404
PspCI CACGTG 1 cut(s) 1227
PspEI GGTNACC 1 cut(s) 929
PspFI CCCAGC 1 cut(s) 1135
PspN4I GGNNCC 1 cut(s) 433
PspPI GGNCC 2 cut(s) 211, 224
PstI CTGCAG 1 cut(s) 170
PsuI RGATCY 2 cut(s) 562, 849
RsaI GTAC 5 cut(s) 124, 201, 426, 881, 1148
RsaNI GTAC 5 cut(s) 123, 200, 425, 880, 1147
RseI CAYNNNNRTG 1 cut(s) 64
SaqAI TTAA 1 cut(s) 740
SatI GCNGC 6 cut(s) 505, 639, 784, 787, 989, 1157
Sau3AI GATC 3 cut(s) 271, 562, 849
Sau96I GGNCC 2 cut(s) 211, 224
SchI GAGTC 4 cut(s) 327, 341, 1066, 1103
SduI GDGCHC 1 cut(s) 467
SfaNI GCATC 3 cut(s) 203, 825, 1050
SfcI CTRYAG 1 cut(s) 166
SinI GGWCC 1 cut(s) 211
SmiMI CAYNNNNRTG 1 cut(s) 64
SmlI CTYRAG 2 cut(s) 299, 1005
SmoI CTYRAG 2 cut(s) 299, 1005
Sse9I AATT 3 cut(s) 72, 358, 1068
SsiI CCGC 9 cut(s) 408, 621, 657, 666, 703, 786, 870, 989, 1159
SspI AATATT 1 cut(s) 1323
SspMI CTAG 2 cut(s) 18, 654
StyI CCWWGG 1 cut(s) 799
TaaI ACNGT 7 cut(s) 133, 356, 400, 525, 693, 1120, 1300
TaiI ACGT 5 cut(s) 124, 1229, 1308, 1407, 1440
TaqI TCGA 7 cut(s) 495, 573, 841, 1088, 1107, 1171, 1205
TaqII GACCGA 2 cut(s) 821, 827
TasI AATT 3 cut(s) 72, 358, 1068
TatI WGTACW 1 cut(s) 879
TauI GCSGC 2 cut(s) 789, 991
TfiI GAWTC 4 cut(s) 366, 403, 888, 1245
Tru1I TTAA 1 cut(s) 740
Tru9I TTAA 1 cut(s) 740
TscAI CASTG 4 cut(s) 457, 530, 931, 1246
TseI GCWGC 4 cut(s) 504, 638, 783, 1156
TspDTI ATGAA 2 cut(s) 294, 1196
TspGWI ACGGA 1 cut(s) 187
TspRI CASTG 4 cut(s) 457, 530, 931, 1246
VpaK11BI GGWCC 1 cut(s) 211
XmiI GTMKAC 2 cut(s) 681, 1302
XspI CTAG 2 cut(s) 18, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.