pycom08g04240

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000845
Physical Location & Seq
Reverse (-)
3273492 .. 3274682
1191 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g04240.1

Sequence Viewer

Length: 963 bp
ATGTTACCTTGTGATGAGAGCAAGAAGTCATCATCTTCATCGGTACCAAAAGTGGTACACAAGCACGGACCATGCTCTCGAATCAACAAGAACAAATCCAAAACTCCAACTCATGCCCAAATCCTCAACCGGGACCAAGCCCGAGTCGACTCAATCCACTCCCGATTCAACACCAAGAAATCACTATCTACAACCACTGTCGGTAAACTCCGTCCATCCGCCGCCACCACCATCCCGGCCCAGTCCGGAATAGTAGTGGGATCGGGCAATTACATTGTCAATGTCGGCCTGCGCTCCCCCAAGAAGCAGCTCCCCCTCGTTTTCGACCCCGCAACCTCCGCCTCATACATGAACACGTCTCCTGCAATTCCAGCTGAGGTCAGCCACAGGATACACCCCCCGCTGCTCCACCACCTCCACCTGCTGAGCCTGCCGTCGACGGATGTTTTTAACTGGTTTTTCTTCGGGTGCGGCCAGAGAAACAAAGGCCACTTCTGCGAAACCGCCGGTTTGCTCGGTCTGGGCCGGAATAAAATCTCCTTCGTCGAGCAATCCGCCTTGAAATACAAACGGTTCTTCTCCTACTGCCTCCCCTCCACATCAAGCGGCACCGGATACCTCAGGTTCGGCAAAGGAAGCGAATCTTACAAGGCCGTGGAATTCACGCCCTTCGCCGACATTTCTGAAAGCGGGTCGTTTTACGGCCTCGACGTCGTCGGGATCAACGTTGGCGGGAAAACATTACCTATTTCCCGTACAATTTTTTCCTCCTCGGGGACGATTATAGATTCTGGGACGGTGATCACGCGCCTGCCGGCTGCGGTGTACACGGCACTGCGGGACGCGTTTCGGCAGGGGATGAAGAGCTACACGTCGGCTCCGGCGCTTTCTATTCTCGACACTTGCTACGACTTCATCAACGTCGAAACGGTGACGTATCCGAAGATTGCGTTGGCTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

321

Amino Acids

34.71

Weight (kDa)

9.71

Isoelectric Point (pI)

44.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 148 - 211 1.9e-16 Xylanase inhibitor N-terminal
Asp PF00026 166 - 288 2.2e-06 Eukaryotic aspartyl protease
TAXi_C PF14541 234 - 320 6.6e-14 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 429
AatII GACGTC 1 cut(s) 714
Acc36I ACCTGC 1 cut(s) 429
Acc65I GGTACC 1 cut(s) 43
AccB1I GGYRCC 2 cut(s) 43, 608
AccI GTMKAC 2 cut(s) 147, 437
AccII CGCG 2 cut(s) 808, 845
AccIII TCCGGA 1 cut(s) 245
AclI AACGTT 1 cut(s) 726
AclWI GGATC 2 cut(s) 268, 728
AcoI YGGCCR 1 cut(s) 472
AcsI RAATTY 1 cut(s) 659
AcyI GRCGYC 1 cut(s) 711
AfaI GTAC 4 cut(s) 45, 57, 757, 827
AfiI CCNNNNNNNGG 2 cut(s) 130, 774
AflIII ACRYGT 3 cut(s) 354, 843, 870
AgsI TTSAA 2 cut(s) 169, 562
AjiI CACGTC 2 cut(s) 357, 873
AjuI GAANNNNNNNTTGG 1 cut(s) 935
AluBI AGCT 3 cut(s) 310, 374, 867
AluI AGCT 3 cut(s) 310, 374, 867
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 2 cut(s) 268, 728
Ama87I CYCGRG 2 cut(s) 141, 772
Aor13HI TCCGGA 1 cut(s) 245
AoxI GGCC 7 cut(s) 237, 286, 472, 487, 523, 651, 703
ApeKI GCWGC 3 cut(s) 307, 403, 818
ApoI RAATTY 1 cut(s) 659
Asp718I GGTACC 1 cut(s) 43
AspLEI GCGC 3 cut(s) 294, 810, 886
AspS9I GGNCC 4 cut(s) 68, 133, 238, 523
AsuC2I CCSGG 2 cut(s) 131, 236
AsuHPI GGTGA 2 cut(s) 811, 943
AvaI CYCGRG 2 cut(s) 141, 772
AvaII GGWCC 2 cut(s) 68, 133
AxyI CCTNAGG 1 cut(s) 620
BanI GGYRCC 2 cut(s) 43, 608
BbvCI CCTCAGC 1 cut(s) 375
BbvI GCAGC 3 cut(s) 319, 390, 805
BccI CCATC 2 cut(s) 223, 239
BceAI ACGGC 4 cut(s) 418, 638, 718, 846
BciVI GTATCC 3 cut(s) 384, 608, 948
BclI TGATCA 1 cut(s) 801
BcnI CCSGG 2 cut(s) 131, 236
BcoDI GTCTC 1 cut(s) 363
BfaI CTAG 1 cut(s) 961
BfoI RGCGCY 1 cut(s) 887
BfuAI ACCTGC 1 cut(s) 429
BfuI GTATCC 3 cut(s) 384, 608, 948
BisI GCNGC 6 cut(s) 222, 308, 404, 472, 607, 819
BlpI GCTNAGC 1 cut(s) 425
BlsI GCNGC 6 cut(s) 223, 309, 405, 473, 608, 820
Bme1390I CCNGG 2 cut(s) 131, 236
Bme18I GGWCC 2 cut(s) 68, 133
BmeT110I CYCGRG 2 cut(s) 141, 772
BmgBI CACGTC 2 cut(s) 357, 873
BmgT120I GGNCC 4 cut(s) 68, 133, 238, 523
BmiI GGNNCC 4 cut(s) 45, 134, 610, 879
BmrFI CCNGG 2 cut(s) 131, 236
BmrI ACTGGG 1 cut(s) 235
BmuI ACTGGG 1 cut(s) 235
Bpu10I CCTNAGC 1 cut(s) 375
Bpu1102I GCTNAGC 1 cut(s) 425
BpuMI CCSGG 2 cut(s) 131, 236
BsaHI GRCGYC 1 cut(s) 711
BsaJI CCNNGG 2 cut(s) 654, 771
BsaWI WCCGGW 2 cut(s) 245, 611
BsaXI ACNNNNNCTCC 2 cut(s) 862, 892
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 774
Bse118I RCCGGY 2 cut(s) 506, 814
Bse1I ACTGG 2 cut(s) 241, 458
Bse21I CCTNAGG 1 cut(s) 620
BseAI TCCGGA 1 cut(s) 245
BseDI CCNNGG 2 cut(s) 654, 771
BseGI GGATG 4 cut(s) 215, 231, 448, 864
BseLI CCNNNNNNNGG 2 cut(s) 130, 774
BseMII CTCAG 3 cut(s) 366, 416, 634
BseNI ACTGG 2 cut(s) 241, 458
BseRI GAGGAG 1 cut(s) 760
BseXI GCAGC 3 cut(s) 319, 390, 805
Bsh1236I CGCG 2 cut(s) 808, 845
BshFI GGCC 7 cut(s) 239, 288, 474, 489, 525, 653, 705
BshNI GGYRCC 2 cut(s) 43, 608
BsiHKCI CYCGRG 2 cut(s) 141, 772
BsiSI CCGG 8 cut(s) 130, 236, 246, 507, 526, 612, 815, 881
BslFI GGGAC 4 cut(s) 146, 790, 808, 854
BslI CCNNNNNNNGG 2 cut(s) 130, 774
BsmAI GTCTC 1 cut(s) 363
BsmBI CGTCTC 1 cut(s) 363
BsmFI GGGAC 4 cut(s) 146, 790, 808, 854
BsnI GGCC 7 cut(s) 239, 288, 474, 489, 525, 653, 705
BsoBI CYCGRG 2 cut(s) 141, 772
Bsp13I TCCGGA 1 cut(s) 245
Bsp1407I TGTACA 1 cut(s) 825
Bsp143I GATC 3 cut(s) 260, 720, 801
Bsp1720I GCTNAGC 1 cut(s) 425
BspANI GGCC 7 cut(s) 239, 288, 474, 489, 525, 653, 705
BspCNI CTCAG 3 cut(s) 367, 417, 633
BspEI TCCGGA 1 cut(s) 245
BspFNI CGCG 2 cut(s) 808, 845
BspLI GGNNCC 4 cut(s) 45, 134, 610, 879
BspMI ACCTGC 1 cut(s) 429
BspPI GGATC 2 cut(s) 268, 728
BspQI GCTCTTC 1 cut(s) 857
BspT107I GGYRCC 2 cut(s) 43, 608
BsrFI RCCGGY 2 cut(s) 506, 814
BsrGI TGTACA 1 cut(s) 825
BsrI ACTGG 2 cut(s) 241, 458
BssAI RCCGGY 2 cut(s) 506, 814
BssECI CCNNGG 2 cut(s) 654, 771
BssMI GATC 3 cut(s) 260, 720, 801
BssNI GRCGYC 1 cut(s) 711
Bst4CI ACNGT 4 cut(s) 199, 573, 799, 931
Bst6I CTCTTC 1 cut(s) 857
BstACI GRCGYC 1 cut(s) 711
BstAUI TGTACA 1 cut(s) 825
BstC8I GCNNGC 4 cut(s) 290, 431, 812, 816
BstDEI CTNAG 3 cut(s) 375, 425, 620
BstDSI CCRYGG 1 cut(s) 654
BstF5I GGATG 4 cut(s) 215, 231, 448, 864
BstFNI CGCG 2 cut(s) 808, 845
BstH2I RGCGCY 1 cut(s) 887
BstHHI GCGC 3 cut(s) 294, 810, 886
BstKTI GATC 3 cut(s) 263, 723, 804
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 3 cut(s) 260, 720, 801
BstMWI GCNNNNNNNGC 5 cut(s) 338, 371, 430, 495, 636
BstSCI CCNGG 2 cut(s) 129, 234
BstUI CGCG 2 cut(s) 808, 845
BstV1I GCAGC 3 cut(s) 319, 390, 805
Bsu36I CCTNAGG 1 cut(s) 620
BsuI GTATCC 3 cut(s) 384, 608, 948
BsuRI GGCC 7 cut(s) 239, 288, 474, 489, 525, 653, 705
BtgI CCRYGG 1 cut(s) 654
BtrI CACGTC 2 cut(s) 357, 873
BtsCI GGATG 4 cut(s) 215, 231, 448, 864
BtsI GCAGTG 1 cut(s) 833
BtsIMutI CAGTG 2 cut(s) 195, 833
BveI ACCTGC 1 cut(s) 429
Cac8I GCNNGC 4 cut(s) 290, 431, 812, 816
CfoI GCGC 3 cut(s) 294, 810, 886
Cfr10I RCCGGY 2 cut(s) 506, 814
Cfr13I GGNCC 4 cut(s) 68, 133, 238, 523
CseI GACGC 1 cut(s) 851
Csp6I GTAC 4 cut(s) 44, 56, 756, 826
CviAII CATG 3 cut(s) 72, 113, 349
CviQI GTAC 4 cut(s) 44, 56, 756, 826
DdeI CTNAG 3 cut(s) 375, 425, 620
DpnI GATC 3 cut(s) 262, 722, 803
DpnII GATC 3 cut(s) 260, 720, 801
EaeI YGGCCR 1 cut(s) 472
Eam1104I CTCTTC 1 cut(s) 857
EarI CTCTTC 1 cut(s) 857
EciI GGCGGA 3 cut(s) 208, 328, 544
Eco47I GGWCC 2 cut(s) 68, 133
Eco81I CCTNAGG 1 cut(s) 620
Eco88I CYCGRG 2 cut(s) 141, 772
EcoRI GAATTC 1 cut(s) 659
Esp3I CGTCTC 1 cut(s) 363
FaeI CATG 3 cut(s) 75, 116, 352
FaiI YATR 5 cut(s) 73, 114, 346, 350, 785
FalI AAGNNNNNCTT 2 cut(s) 628, 660
FaqI GGGAC 4 cut(s) 146, 790, 808, 854
FatI CATG 3 cut(s) 71, 112, 348
FauI CCCGC 5 cut(s) 337, 408, 683, 725, 831
FbaI TGATCA 1 cut(s) 801
FblI GTMKAC 2 cut(s) 147, 437
Fnu4HI GCNGC 6 cut(s) 222, 308, 404, 472, 607, 819
FokI GGATG 4 cut(s) 202, 218, 455, 871
Fsp4HI GCNGC 6 cut(s) 222, 308, 404, 472, 607, 819
FspBI CTAG 1 cut(s) 961
GlaI GCGC 3 cut(s) 293, 809, 885
GluI GCNGC 6 cut(s) 222, 308, 404, 472, 607, 819
HaeII RGCGCY 1 cut(s) 887
HaeIII GGCC 7 cut(s) 239, 288, 474, 489, 525, 653, 705
HapII CCGG 8 cut(s) 130, 236, 246, 507, 526, 612, 815, 881
HgaI GACGC 1 cut(s) 851
HhaI GCGC 3 cut(s) 294, 810, 886
Hin1I GRCGYC 1 cut(s) 711
Hin1II CATG 3 cut(s) 75, 116, 352
Hin6I GCGC 3 cut(s) 292, 808, 884
HinP1I GCGC 3 cut(s) 292, 808, 884
HincII GTYRAC 2 cut(s) 148, 438
HindII GTYRAC 2 cut(s) 148, 438
HinfI GANTC 6 cut(s) 81, 144, 149, 165, 641, 788
HpaII CCGG 8 cut(s) 130, 236, 246, 507, 526, 612, 815, 881
HphI GGTGA 2 cut(s) 811, 943
Hpy166II GTNNAC 6 cut(s) 58, 148, 206, 438, 826, 828
Hpy188I TCNGA 2 cut(s) 685, 942
Hpy188III TCNNGA 5 cut(s) 78, 162, 246, 718, 896
Hpy8I GTNNAC 6 cut(s) 58, 148, 206, 438, 826, 828
Hpy99I CGWCG 8 cut(s) 439, 442, 548, 713, 716, 719, 877, 926
HpyAV CCTTC 2 cut(s) 550, 679
HpyCH4III ACNGT 4 cut(s) 199, 573, 799, 931
HpyCH4IV ACGT 6 cut(s) 356, 711, 726, 872, 921, 935
HpyCH4V TGCA 1 cut(s) 365
HpyF10VI GCNNNNNNNGC 5 cut(s) 338, 371, 430, 495, 636
HpyF3I CTNAG 3 cut(s) 375, 425, 620
HpySE526I ACGT 6 cut(s) 356, 711, 726, 872, 921, 935
Hsp92I GRCGYC 1 cut(s) 711
Hsp92II CATG 3 cut(s) 75, 116, 352
HspAI GCGC 3 cut(s) 292, 808, 884
Kpn2I TCCGGA 1 cut(s) 245
KpnI GGTACC 1 cut(s) 47
KroI GCCGGC 1 cut(s) 814
KroNI GCCGGC 1 cut(s) 816
Ksp22I TGATCA 1 cut(s) 801
Kzo9I GATC 3 cut(s) 260, 720, 801
LguI GCTCTTC 1 cut(s) 857
LmnI GCTCC 4 cut(s) 299, 315, 411, 883
Lsp1109I GCAGC 3 cut(s) 319, 390, 805
MaeI CTAG 1 cut(s) 961
MaeII ACGT 6 cut(s) 356, 711, 726, 872, 921, 935
MaeIII GTNAC 2 cut(s) 3, 931
MalI GATC 3 cut(s) 262, 722, 803
MboI GATC 3 cut(s) 260, 720, 801
MboII GAAGA 5 cut(s) 27, 454, 568, 874, 955
MluCI AATT 4 cut(s) 268, 366, 659, 759
MluI ACGCGT 1 cut(s) 843
MlyI GAGTC 2 cut(s) 143, 153
MmeI TCCRAC 1 cut(s) 131
MroI TCCGGA 1 cut(s) 245
MroNI GCCGGC 1 cut(s) 814
MseI TTAA 1 cut(s) 450
MspA1I CMGCKG 2 cut(s) 374, 403
MspI CCGG 8 cut(s) 130, 236, 246, 507, 526, 612, 815, 881
MspR9I CCNGG 2 cut(s) 131, 236
MvnI CGCG 2 cut(s) 808, 845
MwoI GCNNNNNNNGC 5 cut(s) 338, 371, 430, 495, 636
NaeI GCCGGC 1 cut(s) 816
NciI CCSGG 2 cut(s) 131, 236
NdeII GATC 3 cut(s) 260, 720, 801
NgoMIV GCCGGC 1 cut(s) 814
NlaIII CATG 3 cut(s) 75, 116, 352
NlaIV GGNNCC 4 cut(s) 45, 134, 610, 879
NmuCI GTSAC 1 cut(s) 931
PaqCI CACCTGC 1 cut(s) 429
PciSI GCTCTTC 1 cut(s) 857
PcsI WCGNNNNNNNCGW 2 cut(s) 708, 723
PdiI GCCGGC 1 cut(s) 816
PfeI GAWTC 4 cut(s) 81, 165, 641, 788
PflFI GACNNNGTC 1 cut(s) 713
PkrI GCNGC 6 cut(s) 223, 309, 405, 473, 608, 820
PleI GAGTC 2 cut(s) 143, 152
PpsI GAGTC 2 cut(s) 143, 152
Psp1406I AACGTT 1 cut(s) 726
PspN4I GGNNCC 4 cut(s) 45, 134, 610, 879
PspPI GGNCC 4 cut(s) 68, 133, 238, 523
PsyI GACNNNGTC 1 cut(s) 713
PvuII CAGCTG 1 cut(s) 374
RsaI GTAC 4 cut(s) 45, 57, 757, 827
RsaNI GTAC 4 cut(s) 44, 56, 756, 826
SalI GTCGAC 2 cut(s) 146, 436
SapI GCTCTTC 1 cut(s) 857
SaqAI TTAA 1 cut(s) 450
SatI GCNGC 6 cut(s) 222, 308, 404, 472, 607, 819
Sau3AI GATC 3 cut(s) 260, 720, 801
Sau96I GGNCC 4 cut(s) 68, 133, 238, 523
SchI GAGTC 2 cut(s) 143, 153
ScrFI CCNGG 2 cut(s) 131, 236
SgrDI CGTCGACG 1 cut(s) 436
SinI GGWCC 2 cut(s) 68, 133
Sse9I AATT 4 cut(s) 268, 366, 659, 759
SspMI CTAG 1 cut(s) 961
StyD4I CCNGG 2 cut(s) 129, 234
TaaI ACNGT 4 cut(s) 199, 573, 799, 931
TaiI ACGT 6 cut(s) 359, 714, 729, 875, 924, 938
TaqI TCGA 8 cut(s) 79, 147, 324, 437, 546, 708, 897, 924
TaqII GACCGA 1 cut(s) 506
TasI AATT 4 cut(s) 268, 366, 659, 759
TatI WGTACW 1 cut(s) 825
TauI GCSGC 3 cut(s) 224, 474, 609
TfiI GAWTC 4 cut(s) 81, 165, 641, 788
Tru1I TTAA 1 cut(s) 450
Tru9I TTAA 1 cut(s) 450
TscAI CASTG 2 cut(s) 202, 840
TseFI GTSAC 1 cut(s) 931
TseI GCWGC 3 cut(s) 307, 403, 818
Tsp45I GTSAC 1 cut(s) 931
TspDTI ATGAA 4 cut(s) 27, 365, 875, 904
TspGWI ACGGA 3 cut(s) 81, 200, 455
TspRI CASTG 2 cut(s) 202, 840
Tth111I GACNNNGTC 1 cut(s) 713
VpaK11BI GGWCC 2 cut(s) 68, 133
XapI RAATTY 1 cut(s) 659
XmiI GTMKAC 2 cut(s) 147, 437
XspI CTAG 1 cut(s) 961
ZraI GACGTC 1 cut(s) 712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.