Prupe.4G162100_v2.0.a1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000845
Physical Location & Seq
Reverse (-)
9299112 .. 9301208
2097 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G162100.1

Sequence Viewer

Length: 1293 bp
ATGAAAACTCTTAATATTTCCACCTCCTGCTTCCTCAAGTATTTCATTCGTTGCTTTCTTCTTCTATGCCTGAATCTATTCCCTTCGGAACCTGGCATTTCCTTGGCAGAAAGAGACATGGAAACTGACCTTGCTGTTCAACATATTCATAGAGTTGAAGTCAAGTCTCTGCTACCCGAAACCACCTGCAGCCCCTCCACCAAAGACCAAGCCGGGGTCATATCAATCCACTCTCGGCTTGGTTCAAAGAAAATGAACAGCGACGATGCCACCACAATGCCAGTCAATTCCGGTGACTTAGTGAGTTCTGGGGACTACATTGTGACTGTGGGCCTAGGCACACCTCAGAAGCAACTTTCGCTCATATTTGACACAGGAAGCGATCTCTCCTGGACTCGATGTCGGCCATGCATTCGATCTTGCTACAAACAAATGGAGCCCCTCTTTGACCCTTCCATCTCCACTTCATATGTCAATATTTCCTGTAAATCCAATGTCTGCTCTCAGCTCACTCCAGTCACACACAAAATACCTCGTTGCTCAATTGACAACTCTACGTGTATATACGACATGCAATATGGAGACAAATCATTCTCTGTTGGATTTTTCAGCAAAGAAAGACTCACCTTAACTTCAACTGAGGTGTTCGATGAGTTCCTCTTTGGATGCGGCCAAATCAACCAAGGCAACTTTGGGAGCTCCGCCGCCAACAAGTATGGCCGCATTTTCTCTTATTGTCTACCCTCCACTTCAAGCTCCACCGGTTACCTTGGTTTTGGCAAACACCGCAGAGCTTTTAAGCCCGTAAAGTTCACGCCCCTCACCACTGTCTCTCAAGACCCATCTTTTTATGGCCTCAACCTGGTCGGTATCAGTGTTGGTGGACATAAATTGTGGATTTCACCTTCGGTTTTCTTATCTTCTGGGACCATCATTGACACGGGGACTGTCATAACCCGCTTGCCTGCGGCGGCTTACAGCGCTTTGCGGGATGTGTTTCGTCAAGCGTTGACAAAATATCCGTTAACTCGGGCACTTTCGGTACTGGATAAATGCTATGATCTTAGTAGTTATGTGACAGTGAAGTATCCACACATAGCATTTTACTTTCAGGGAGGGCTGAAGTTGAAGTTGGATGCTACGGGTATATTTTACTCATCCAGTGCTTCCCAGGTTTGCTTGGCCTTTGCTGGAAATGATGCTAATAATAAATTTGCAGTACTTGGAAATGTTCAGCAGAAGACGTTCGAGGCTGGGGGTAGGGTTGGATTTGCCCGTGGAGGTTGCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

431

Amino Acids

47.01

Weight (kDa)

8.89

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 194
Acc36I ACCTGC 1 cut(s) 194
AccI GTMKAC 1 cut(s) 739
AciI CCGC 9 cut(s) 669, 702, 705, 721, 787, 958, 968, 971, 988
AcoI YGGCCR 3 cut(s) 404, 670, 718
AcsI RAATTY 1 cut(s) 1211
AcuI CTGAAG 1 cut(s) 1142
AfaI GTAC 2 cut(s) 1044, 1221
AfeI AGCGCT 1 cut(s) 982
AfiI CCNNNNNNNGG 2 cut(s) 214, 862
AflIII ACRYGT 1 cut(s) 557
AgeI ACCGGT 1 cut(s) 761
AgsI TTSAA 6 cut(s) 140, 158, 246, 636, 753, 1129
AhdI GACNNNNNGTC 1 cut(s) 399
AjnI CCWGG 4 cut(s) 91, 389, 861, 1170
AjuI GAANNNNNNNTTGG 2 cut(s) 1115, 1147
AluBI AGCT 4 cut(s) 508, 699, 756, 794
AluI AGCT 4 cut(s) 508, 699, 756, 794
Alw21I GWGCWC 1 cut(s) 701
Alw26I GTCTC 4 cut(s) 108, 171, 576, 835
Ama87I CYCGRG 1 cut(s) 1029
Aor51HI AGCGCT 1 cut(s) 982
AoxI GGCC 6 cut(s) 331, 404, 670, 718, 853, 1182
ApeKI GCWGC 1 cut(s) 189
ApoI RAATTY 1 cut(s) 1211
AsiGI ACCGGT 1 cut(s) 761
Asp700I GAANNNNTTC 2 cut(s) 77, 1244
AspA2I CCTAGG 1 cut(s) 334
AspLEI GCGC 1 cut(s) 983
AspS9I GGNCC 2 cut(s) 331, 927
AsuC2I CCSGG 1 cut(s) 214
AsuHPI GGTGA 4 cut(s) 305, 616, 814, 894
AvaI CYCGRG 1 cut(s) 1029
AvaII GGWCC 1 cut(s) 927
AvrII CCTAGG 1 cut(s) 334
BaeGI GKGCMC 1 cut(s) 1036
BanII GRGCYC 2 cut(s) 441, 701
BbsI GAAGAC 1 cut(s) 1247
Bbv12I GWGCWC 1 cut(s) 701
BbvI GCAGC 1 cut(s) 201
BccI CCATC 3 cut(s) 464, 850, 938
BciT130I CCWGG 4 cut(s) 93, 391, 863, 1172
BciVI GTATCC 1 cut(s) 1098
BcnI CCSGG 1 cut(s) 214
BcoDI GTCTC 4 cut(s) 108, 171, 576, 835
BfaI CTAG 1 cut(s) 335
BfmI CTRYAG 1 cut(s) 187
BfoI RGCGCY 1 cut(s) 984
BfuAI ACCTGC 1 cut(s) 194
BfuI GTATCC 1 cut(s) 1098
BisI GCNGC 6 cut(s) 190, 670, 705, 721, 969, 972
BlnI CCTAGG 1 cut(s) 334
BlsI GCNGC 6 cut(s) 191, 671, 706, 722, 970, 973
BmcAI AGTACT 1 cut(s) 1221
Bme1390I CCNGG 5 cut(s) 93, 214, 391, 863, 1172
Bme18I GGWCC 1 cut(s) 927
BmeRI GACNNNNNGTC 1 cut(s) 399
BmeT110I CYCGRG 1 cut(s) 1029
BmgT120I GGNCC 2 cut(s) 331, 927
BmiI GGNNCC 3 cut(s) 90, 438, 928
BmrFI CCNGG 5 cut(s) 93, 214, 391, 863, 1172
BmsI GCATC 4 cut(s) 256, 656, 1126, 1189
BpiI GAAGAC 1 cut(s) 1247
BpmI CTGGAG 1 cut(s) 498
BpuEI CTTGAG 2 cut(s) 20, 819
BpuMI CCSGG 1 cut(s) 214
BsaAI YACGTR 1 cut(s) 558
BsaJI CCNNGG 7 cut(s) 102, 213, 334, 682, 769, 1170, 1276
BsaWI WCCGGW 2 cut(s) 290, 761
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 862
Bse118I RCCGGY 1 cut(s) 761
Bse1I ACTGG 4 cut(s) 281, 515, 1050, 1161
BseBI CCWGG 4 cut(s) 93, 391, 863, 1172
BseDI CCNNGG 7 cut(s) 102, 213, 334, 682, 769, 1170, 1276
BseGI GGATG 4 cut(s) 671, 997, 1141, 1157
BseLI CCNNNNNNNGG 2 cut(s) 214, 862
BseMII CTCAG 3 cut(s) 359, 518, 630
BseNI ACTGG 4 cut(s) 281, 515, 1050, 1161
BseSI GKGCMC 1 cut(s) 1036
BseXI GCAGC 1 cut(s) 201
BseYI CCCAGC 1 cut(s) 1253
BshFI GGCC 6 cut(s) 333, 406, 672, 720, 855, 1184
BshTI ACCGGT 1 cut(s) 761
BsiHKAI GWGCWC 1 cut(s) 701
BsiHKCI CYCGRG 1 cut(s) 1029
BsiSI CCGG 3 cut(s) 213, 291, 762
BslFI GGGAC 3 cut(s) 326, 940, 958
BslI CCNNNNNNNGG 2 cut(s) 214, 862
BsmAI GTCTC 4 cut(s) 108, 171, 576, 835
BsmFI GGGAC 3 cut(s) 326, 940, 958
BsmI GAATGC 1 cut(s) 411
BsnI GGCC 6 cut(s) 333, 406, 672, 720, 855, 1184
BsoBI CYCGRG 1 cut(s) 1029
Bsp1286I GDGCHC 3 cut(s) 441, 701, 1036
Bsp143I GATC 3 cut(s) 382, 416, 1060
BspACI CCGC 9 cut(s) 669, 702, 705, 721, 787, 958, 968, 971, 988
BspANI GGCC 6 cut(s) 333, 406, 672, 720, 855, 1184
BspCNI CTCAG 3 cut(s) 358, 517, 631
BspLI GGNNCC 3 cut(s) 90, 438, 928
BspMAI CTGCAG 1 cut(s) 191
BspMI ACCTGC 1 cut(s) 194
BsrFI RCCGGY 1 cut(s) 761
BsrI ACTGG 4 cut(s) 281, 515, 1050, 1161
BssAI RCCGGY 1 cut(s) 761
BssECI CCNNGG 7 cut(s) 102, 213, 334, 682, 769, 1170, 1276
BssMI GATC 3 cut(s) 382, 416, 1060
BssT1I CCWWGG 4 cut(s) 102, 334, 682, 769
Bst2UI CCWGG 4 cut(s) 93, 391, 863, 1172
Bst4CI ACNGT 4 cut(s) 328, 829, 949, 1081
BstBAI YACGTR 1 cut(s) 558
BstC8I GCNNGC 2 cut(s) 962, 966
BstDEI CTNAG 5 cut(s) 298, 345, 504, 639, 1064
BstDSI CCRYGG 1 cut(s) 1276
BstEII GGTNACC 1 cut(s) 764
BstF5I GGATG 4 cut(s) 671, 997, 1141, 1157
BstH2I RGCGCY 1 cut(s) 984
BstHHI GCGC 1 cut(s) 983
BstKTI GATC 3 cut(s) 385, 419, 1063
BstMAI GTCTC 4 cut(s) 108, 171, 576, 835
BstMBI GATC 3 cut(s) 382, 416, 1060
BstMWI GCNNNNNNNGC 3 cut(s) 358, 786, 980
BstNI CCWGG 4 cut(s) 93, 391, 863, 1172
BstNSI RCATGY 1 cut(s) 574
BstPI GGTNACC 1 cut(s) 764
BstSCI CCNGG 5 cut(s) 91, 212, 389, 861, 1170
BstSFI CTRYAG 1 cut(s) 187
BstSLI GKGCMC 1 cut(s) 1036
BstV1I GCAGC 1 cut(s) 201
BstV2I GAAGAC 1 cut(s) 1247
BsuI GTATCC 1 cut(s) 1098
BsuRI GGCC 6 cut(s) 333, 406, 672, 720, 855, 1184
BtgI CCRYGG 1 cut(s) 1276
BtsCI GGATG 4 cut(s) 671, 997, 1141, 1157
BtsIMutI CAGTG 4 cut(s) 825, 880, 1086, 1168
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 2 cut(s) 962, 966
CfoI GCGC 1 cut(s) 983
Cfr10I RCCGGY 1 cut(s) 761
Cfr13I GGNCC 2 cut(s) 331, 927
CsiI ACCWGGT 1 cut(s) 861
Csp6I GTAC 2 cut(s) 1043, 1220
CspAI ACCGGT 1 cut(s) 761
CviAII CATG 3 cut(s) 118, 408, 571
CviQI GTAC 2 cut(s) 1043, 1220
DdeI CTNAG 5 cut(s) 298, 345, 504, 639, 1064
DpnI GATC 3 cut(s) 384, 418, 1062
DpnII GATC 3 cut(s) 382, 416, 1060
DriI GACNNNNNGTC 1 cut(s) 399
EaeI YGGCCR 3 cut(s) 404, 670, 718
Eam1105I GACNNNNNGTC 1 cut(s) 399
EciI GGCGGA 1 cut(s) 691
Ecl136II GAGCTC 1 cut(s) 699
Eco130I CCWWGG 4 cut(s) 102, 334, 682, 769
Eco24I GRGCYC 2 cut(s) 441, 701
Eco47I GGWCC 1 cut(s) 927
Eco47III AGCGCT 1 cut(s) 982
Eco53kI GAGCTC 1 cut(s) 699
Eco57I CTGAAG 1 cut(s) 1142
Eco88I CYCGRG 1 cut(s) 1029
Eco91I GGTNACC 1 cut(s) 764
EcoICRI GAGCTC 1 cut(s) 699
EcoO65I GGTNACC 1 cut(s) 764
EcoRII CCWGG 4 cut(s) 91, 389, 861, 1170
EcoT14I CCWWGG 4 cut(s) 102, 334, 682, 769
EcoT22I ATGCAT 1 cut(s) 413
EcoT38I GRGCYC 2 cut(s) 441, 701
ErhI CCWWGG 4 cut(s) 102, 334, 682, 769
FaeI CATG 3 cut(s) 121, 411, 574
FaqI GGGAC 3 cut(s) 326, 940, 958
FatI CATG 3 cut(s) 117, 407, 570
FauI CCCGC 2 cut(s) 965, 981
FauNDI CATATG 1 cut(s) 469
FblI GTMKAC 1 cut(s) 739
Fnu4HI GCNGC 6 cut(s) 190, 670, 705, 721, 969, 972
FokI GGATG 4 cut(s) 678, 1004, 1144, 1148
FriOI GRGCYC 2 cut(s) 441, 701
Fsp4HI GCNGC 6 cut(s) 190, 670, 705, 721, 969, 972
FspBI CTAG 1 cut(s) 335
GlaI GCGC 1 cut(s) 982
GluI GCNGC 6 cut(s) 190, 670, 705, 721, 969, 972
GsaI CCCAGC 1 cut(s) 1257
GsuI CTGGAG 1 cut(s) 498
HaeII RGCGCY 1 cut(s) 984
HaeIII GGCC 6 cut(s) 333, 406, 672, 720, 855, 1184
HapII CCGG 3 cut(s) 213, 291, 762
HhaI GCGC 1 cut(s) 983
Hin1II CATG 3 cut(s) 121, 411, 574
Hin6I GCGC 1 cut(s) 981
HinP1I GCGC 1 cut(s) 981
HincII GTYRAC 2 cut(s) 1011, 1026
HindII GTYRAC 2 cut(s) 1011, 1026
HinfI GANTC 3 cut(s) 73, 394, 621
HpaI GTTAAC 1 cut(s) 1026
HpaII CCGG 3 cut(s) 213, 291, 762
HphI GGTGA 4 cut(s) 305, 616, 814, 894
Hpy166II GTNNAC 5 cut(s) 740, 813, 884, 1011, 1026
Hpy188I TCNGA 2 cut(s) 88, 348
Hpy188III TCNNGA 1 cut(s) 836
Hpy8I GTNNAC 5 cut(s) 740, 813, 884, 1011, 1026
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 3 cut(s) 93, 462, 915
HpyCH4III ACNGT 4 cut(s) 328, 829, 949, 1081
HpyCH4IV ACGT 2 cut(s) 557, 1244
HpyCH4V TGCA 4 cut(s) 189, 411, 574, 1217
HpyF10VI GCNNNNNNNGC 3 cut(s) 358, 786, 980
HpyF3I CTNAG 5 cut(s) 298, 345, 504, 639, 1064
HpySE526I ACGT 2 cut(s) 557, 1244
Hsp92II CATG 3 cut(s) 121, 411, 574
HspAI GCGC 1 cut(s) 981
KspAI GTTAAC 1 cut(s) 1026
Kzo9I GATC 3 cut(s) 382, 416, 1060
LmnI GCTCC 4 cut(s) 436, 696, 704, 761
Lsp1109I GCAGC 1 cut(s) 201
LweI GCATC 4 cut(s) 256, 656, 1126, 1189
MabI ACCWGGT 1 cut(s) 861
MaeI CTAG 1 cut(s) 335
MaeII ACGT 2 cut(s) 557, 1244
MaeIII GTNAC 5 cut(s) 293, 322, 517, 764, 1075
MalI GATC 3 cut(s) 384, 418, 1062
MboI GATC 3 cut(s) 382, 416, 1060
MboII GAAGA 4 cut(s) 50, 53, 912, 1252
MfeI CAATTG 1 cut(s) 543
MhlI GDGCHC 3 cut(s) 441, 701, 1036
MluCI AATT 4 cut(s) 286, 543, 890, 1211
MlyI GAGTC 2 cut(s) 388, 615
MmeI TCCRAC 3 cut(s) 580, 1113, 1246
Mph1103I ATGCAT 1 cut(s) 413
MroXI GAANNNNTTC 2 cut(s) 77, 1244
MseI TTAA 4 cut(s) 12, 629, 798, 1025
MslI CAYNNNNRTG 1 cut(s) 275
MspI CCGG 3 cut(s) 213, 291, 762
MspR9I CCNGG 5 cut(s) 93, 214, 391, 863, 1172
MunI CAATTG 1 cut(s) 543
Mva1269I GAATGC 1 cut(s) 411
MvaI CCWGG 4 cut(s) 93, 391, 863, 1172
MwoI GCNNNNNNNGC 3 cut(s) 358, 786, 980
NciI CCSGG 1 cut(s) 214
NdeI CATATG 1 cut(s) 469
NdeII GATC 3 cut(s) 382, 416, 1060
NlaIII CATG 3 cut(s) 121, 411, 574
NlaIV GGNNCC 3 cut(s) 90, 438, 928
NmeAIII GCCGAG 1 cut(s) 214
NmuCI GTSAC 4 cut(s) 293, 322, 517, 1075
NsiI ATGCAT 1 cut(s) 413
NspI RCATGY 1 cut(s) 574
PaqCI CACCTGC 1 cut(s) 194
PctI GAATGC 1 cut(s) 411
PdmI GAANNNNTTC 2 cut(s) 77, 1244
PfeI GAWTC 1 cut(s) 73
PfoI TCCNGGA 1 cut(s) 389
PinAI ACCGGT 1 cut(s) 761
PkrI GCNGC 6 cut(s) 191, 671, 706, 722, 970, 973
PleI GAGTC 2 cut(s) 388, 615
PpsI GAGTC 2 cut(s) 388, 615
Ppu21I YACGTR 1 cut(s) 558
Psp124BI GAGCTC 1 cut(s) 701
Psp6I CCWGG 4 cut(s) 91, 389, 861, 1170
PspEI GGTNACC 1 cut(s) 764
PspFI CCCAGC 1 cut(s) 1253
PspGI CCWGG 4 cut(s) 91, 389, 861, 1170
PspN4I GGNNCC 3 cut(s) 90, 438, 928
PspPI GGNCC 2 cut(s) 331, 927
PstI CTGCAG 1 cut(s) 191
RsaI GTAC 2 cut(s) 1044, 1221
RsaNI GTAC 2 cut(s) 1043, 1220
RseI CAYNNNNRTG 1 cut(s) 275
SacI GAGCTC 1 cut(s) 701
SaqAI TTAA 4 cut(s) 12, 629, 798, 1025
SatI GCNGC 6 cut(s) 190, 670, 705, 721, 969, 972
Sau3AI GATC 3 cut(s) 382, 416, 1060
Sau96I GGNCC 2 cut(s) 331, 927
ScaI AGTACT 1 cut(s) 1221
SchI GAGTC 2 cut(s) 388, 615
ScrFI CCNGG 5 cut(s) 93, 214, 391, 863, 1172
SduI GDGCHC 3 cut(s) 441, 701, 1036
SexAI ACCWGGT 1 cut(s) 861
SfaNI GCATC 4 cut(s) 256, 656, 1126, 1189
SfcI CTRYAG 1 cut(s) 187
SinI GGWCC 1 cut(s) 927
SmiMI CAYNNNNRTG 1 cut(s) 275
SmlI CTYRAG 2 cut(s) 35, 834
SmoI CTYRAG 2 cut(s) 35, 834
Sse9I AATT 4 cut(s) 286, 543, 890, 1211
SsiI CCGC 9 cut(s) 669, 702, 705, 721, 787, 958, 968, 971, 988
SspI AATATT 2 cut(s) 16, 478
SspMI CTAG 1 cut(s) 335
SstI GAGCTC 1 cut(s) 701
StyD4I CCNGG 5 cut(s) 91, 212, 389, 861, 1170
StyI CCWWGG 4 cut(s) 102, 334, 682, 769
TaaI ACNGT 4 cut(s) 328, 829, 949, 1081
TaiI ACGT 2 cut(s) 560, 1247
TaqI TCGA 4 cut(s) 397, 415, 648, 1248
TasI AATT 4 cut(s) 286, 543, 890, 1211
TatI WGTACW 1 cut(s) 1219
TauI GCSGC 5 cut(s) 672, 707, 723, 971, 974
TfiI GAWTC 1 cut(s) 73
Tru1I TTAA 4 cut(s) 12, 629, 798, 1025
Tru9I TTAA 4 cut(s) 12, 629, 798, 1025
TscAI CASTG 4 cut(s) 832, 880, 1086, 1168
TseFI GTSAC 4 cut(s) 293, 322, 517, 1075
TseI GCWGC 1 cut(s) 189
Tsp45I GTSAC 4 cut(s) 293, 322, 517, 1075
TspDTI ATGAA 5 cut(s) 17, 34, 137, 269, 456
TspGWI ACGGA 1 cut(s) 1011
TspRI CASTG 4 cut(s) 832, 880, 1086, 1168
VpaK11BI GGWCC 1 cut(s) 927
XapI RAATTY 1 cut(s) 1211
XceI RCATGY 1 cut(s) 574
XcmI CCANNNNNNNNNTGG 2 cut(s) 236, 689
XmaJI CCTAGG 1 cut(s) 334
XmiI GTMKAC 1 cut(s) 739
XmnI GAANNNNTTC 2 cut(s) 77, 1244
XspI CTAG 1 cut(s) 335
ZrmI AGTACT 1 cut(s) 1221
Zsp2I ATGCAT 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.