Rroxscaffold_5G00347200

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000845
Physical Location & Seq
Forward (+)
18432885 .. 18434770
1886 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00347200.1

Sequence Viewer

Length: 1497 bp
ATGGCAGCTATTAGTCCTAGCTCCTCCTCCTCCTCCTCTTCCACCCCCATTATGAGGTATTTCACCGGTTTGTCTCTTATTCTAAGCCTGACTCTGGGTTCTTTGGACAAGGGCTTTGCCTTGGCAGAAGGAGATTCAAAAGAACGACTCCTTCATCAACCATACACCCACAGAGTTGAACTCCGCTCTCTACTGCCAGCAACCACCTGCAACCCCTCCACCAGAGGTAATAAAAGGATGGCATCCCTTGAAGTGGTACACAAGCATGGGCCATGCTCTCATCTCCCTCAACTCAAAGCGACTGCTATGAATCACACTCTAATTTTTGAGCAAGACCAAGCGCGAGCCAACTCAATCAACTCCCGGATTTCCAAGAAATTGAACGGCAATGAGGATCTAAGCCAATCGGAGGCCACCACCGTCCCGGCCCGGTGGGCAAGCAGCCAAAATGCAGGCAACTACATGGTGACGGTGGGATTGGGCACACCTAAAAAACAACTTTCGCTCGTATTTGACACCGGAAGCGACCTCACTTGGACTCAATGCCAACCTTGTTCTGAATTTTGCTACCCACAAGAGGAGCCCATTTTCGATCCCTCGGCCTCTTCCTCATATACCAACGTTTCCTGTAACAGCACAGAGTGCGTCCAACTCAGGACATCTACGGAATTTGACAAATGCTTAGGAGACACATGCCCATATTCCGTGAGGTACGGAGACTCATCTAACACCACTGGATATTTCGCCACAGACAAGCTGGCCTTAACTCCTAACGACGTATTCGATGGGTTCTTCTTCGGCTGCGGCCAAGAAAACGTAGGTTTCCACGACGGCTCGGCCGGTTTACTTGGTCTCTCCCTCAACAAGTTCTCCCTCGTCGAACAAACCGCACAAAAGTACAGCCGCTTCTTCTCCTACTGTCTGCCACCAACCGCAAGCTCCACCGGTTACATCAGCTTCGGTGCACCCAGTGGAGCTTCCAATGCTGCCGTAAAATACACGCGAATCACTAACTTATCCTCGAGCTTAGAGCTTTATTGCCTAGATCTGGTTGGCATCAATGTGAATGGACAAAAGCTCTCAATTCCTGCTTCGGTGTTTTCGTCGCCGGGGACGATCATCGACTCCGGAACCGTCATATCGCGGCTGCCGTCCACCGCGTATACCGCTTTGCGGGATGCGTTTCGGGCAGCGATGACAAGCTATCCGTTAGTTGCGGGGGATAAATTCCTAGATACATGCTACGACCTTAGTGGCTATCCAACGGTGACGTATCCGAAAATAGAATTTGTGTTCGGCGACGGGGTTACGTTGGAATTGGACGCAACCGGAATTGTATACCTTTTGGACGGTGCGAATAAGCAGGTTTGCTTGGCTTTTGCTGGGAATGATGATGCTAACGACATTACGATTTTCGGGAATACTCAGCAGAAGAATATGGAGGTGTTGTATGACGTTGCCGGAGGGAGAGTTGGATTTGCTCCCGGCGGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

498

Amino Acids

53.44

Weight (kDa)

5.35

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 153 - 495 1.6e-24 Eukaryotic aspartyl protease
TAXi_N PF14543 154 - 321 1.1e-51 Xylanase inhibitor N-terminal
TAXi_C PF14541 346 - 494 5.1e-28 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 215
Acc36I ACCTGC 2 cut(s) 215, 1354
AccBSI CCGCTC 1 cut(s) 186
AccI GTMKAC 2 cut(s) 1163, 1338
AccII CGCG 4 cut(s) 343, 1003, 1144, 1160
AccIII TCCGGA 1 cut(s) 1127
AclI AACGTT 1 cut(s) 621
AclWI GGATC 2 cut(s) 402, 587
AcoI YGGCCR 2 cut(s) 805, 837
AcsI RAATTY 4 cut(s) 560, 668, 1226, 1286
AdeI CACNNNGTG 2 cut(s) 642, 971
AfaI GTAC 3 cut(s) 258, 713, 899
AfiI CCNNNNNNNGG 7 cut(s) 54, 94, 253, 409, 577, 1048, 1173
AgeI ACCGGT 2 cut(s) 65, 944
AgsI TTSAA 4 cut(s) 138, 179, 251, 382
Alw21I GWGCWC 1 cut(s) 967
Alw26I GTCTC 4 cut(s) 78, 681, 711, 857
Alw44I GTGCAC 1 cut(s) 963
AlwI GGATC 2 cut(s) 402, 587
Ama87I CYCGRG 1 cut(s) 1021
Aor13HI TCCGGA 1 cut(s) 1127
AoxI GGCC 7 cut(s) 269, 411, 426, 600, 759, 805, 837
ApaLI GTGCAC 1 cut(s) 963
ApeKI GCWGC 6 cut(s) 5, 441, 801, 986, 1147, 1190
ApoI RAATTY 4 cut(s) 560, 668, 1226, 1286
ArsI GACNNNNNNTTYG 2 cut(s) 98, 130
AsiGI ACCGGT 2 cut(s) 65, 944
AspLEI GCGC 1 cut(s) 343
AspS9I GGNCC 2 cut(s) 269, 427
AsuC2I CCSGG 5 cut(s) 364, 425, 430, 1110, 1485
AsuHPI GGTGA 3 cut(s) 55, 478, 1279
AvaI CYCGRG 1 cut(s) 1021
BaeGI GKGCMC 2 cut(s) 485, 967
BanII GRGCYC 1 cut(s) 585
BarI GAAGNNNNNNTAC 2 cut(s) 890, 922
Bbv12I GWGCWC 1 cut(s) 967
BbvI GCAGC 6 cut(s) 17, 453, 788, 973, 1134, 1202
BccI CCATC 2 cut(s) 232, 779
BceAI ACGGC 4 cut(s) 400, 847, 974, 1135
BciVI GTATCC 1 cut(s) 1284
BcnI CCSGG 5 cut(s) 364, 425, 430, 1110, 1485
BcoDI GTCTC 4 cut(s) 78, 681, 711, 857
BfaI CTAG 3 cut(s) 18, 1043, 1232
BfuAI ACCTGC 2 cut(s) 215, 1354
BfuI GTATCC 1 cut(s) 1284
BglI GCCNNNNNGGC 1 cut(s) 434
BglII AGATCT 1 cut(s) 1045
BisI GCNGC 9 cut(s) 6, 442, 802, 805, 904, 987, 1145, 1148, 1191
BlsI GCNGC 9 cut(s) 7, 443, 803, 806, 905, 988, 1146, 1149, 1192
Bme1390I CCNGG 5 cut(s) 364, 425, 430, 1110, 1485
BmeT110I CYCGRG 1 cut(s) 1021
BmgT120I GGNCC 2 cut(s) 269, 427
BmiI GGNNCC 2 cut(s) 582, 1132
BmrFI CCNGG 5 cut(s) 364, 425, 430, 1110, 1485
BmrI ACTGGG 1 cut(s) 963
BmsI GCATC 4 cut(s) 251, 1065, 1168, 1384
BmuI ACTGGG 1 cut(s) 963
BplI GAGNNNNNCTC 2 cut(s) 165, 197
Bpu10I CCTNAGC 1 cut(s) 682
BpuMI CCSGG 5 cut(s) 364, 425, 430, 1110, 1485
BsaI GGTCTC 1 cut(s) 857
BsaJI CCNNGG 3 cut(s) 120, 597, 1109
BsaWI WCCGGW 5 cut(s) 65, 518, 944, 1127, 1328
BsaXI ACNNNNNCTCC 6 cut(s) 854, 884, 1433, 1455, 1463, 1485
Bsc4I CCNNNNNNNGG 7 cut(s) 54, 94, 253, 409, 577, 1048, 1173
Bse118I RCCGGY 3 cut(s) 65, 839, 944
Bse1I ACTGG 2 cut(s) 739, 969
Bse3DI GCAATG 1 cut(s) 394
BseAI TCCGGA 1 cut(s) 1127
BseDI CCNNGG 3 cut(s) 120, 597, 1109
BseGI GGATG 3 cut(s) 242, 243, 1183
BseLI CCNNNNNNNGG 7 cut(s) 54, 94, 253, 409, 577, 1048, 1173
BseMI GCAATG 1 cut(s) 394
BseMII CTCAG 2 cut(s) 667, 1439
BseNI ACTGG 2 cut(s) 739, 969
BseRI GAGGAG 6 cut(s) 13, 16, 19, 22, 25, 593
BseSI GKGCMC 2 cut(s) 485, 967
BseX3I CGGCCG 1 cut(s) 837
BseXI GCAGC 6 cut(s) 17, 453, 788, 973, 1134, 1202
BseYI CCCAGC 1 cut(s) 1382
Bsh1236I CGCG 4 cut(s) 343, 1003, 1144, 1160
Bsh1285I CGRYCG 1 cut(s) 840
BshFI GGCC 7 cut(s) 271, 413, 428, 602, 761, 807, 839
BshTI ACCGGT 2 cut(s) 65, 944
BsiEI CGRYCG 1 cut(s) 840
BsiHKAI GWGCWC 1 cut(s) 967
BsiHKCI CYCGRG 1 cut(s) 1021
BslFI GGGAC 2 cut(s) 407, 1126
BslI CCNNNNNNNGG 7 cut(s) 54, 94, 253, 409, 577, 1048, 1173
BsmAI GTCTC 4 cut(s) 78, 681, 711, 857
BsmFI GGGAC 2 cut(s) 407, 1126
BsnI GGCC 7 cut(s) 271, 413, 428, 602, 761, 807, 839
Bso31I GGTCTC 1 cut(s) 857
BsoBI CYCGRG 1 cut(s) 1021
Bsp1286I GDGCHC 3 cut(s) 485, 585, 967
Bsp13I TCCGGA 1 cut(s) 1127
Bsp143I GATC 4 cut(s) 394, 592, 1045, 1116
BspANI GGCC 7 cut(s) 271, 413, 428, 602, 761, 807, 839
BspCNI CTCAG 2 cut(s) 666, 1438
BspEI TCCGGA 1 cut(s) 1127
BspFNI CGCG 4 cut(s) 343, 1003, 1144, 1160
BspLI GGNNCC 2 cut(s) 582, 1132
BspMI ACCTGC 2 cut(s) 215, 1354
BspPI GGATC 2 cut(s) 402, 587
BspTNI GGTCTC 1 cut(s) 857
BsrBI CCGCTC 1 cut(s) 186
BsrDI GCAATG 1 cut(s) 394
BsrFI RCCGGY 3 cut(s) 65, 839, 944
BsrI ACTGG 2 cut(s) 739, 969
BssAI RCCGGY 3 cut(s) 65, 839, 944
BssECI CCNNGG 3 cut(s) 120, 597, 1109
BssMI GATC 4 cut(s) 394, 592, 1045, 1116
BssNAI GTATAC 2 cut(s) 1164, 1339
BssT1I CCWWGG 1 cut(s) 120
Bst1107I GTATAC 2 cut(s) 1164, 1339
Bst4CI ACNGT 6 cut(s) 421, 472, 920, 1135, 1267, 1352
Bst6I CTCTTC 2 cut(s) 43, 610
BstAPI GCANNNNNTGC 1 cut(s) 642
BstC8I GCNNGC 6 cut(s) 198, 345, 439, 454, 759, 937
BstDEI CTNAG 7 cut(s) 83, 398, 653, 682, 1027, 1250, 1425
BstF5I GGATG 3 cut(s) 242, 243, 1183
BstFNI CGCG 4 cut(s) 343, 1003, 1144, 1160
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 4 cut(s) 397, 595, 1048, 1119
BstMAI GTCTC 4 cut(s) 78, 681, 711, 857
BstMBI GATC 4 cut(s) 394, 592, 1045, 1116
BstMCI CGRYCG 1 cut(s) 840
BstMWI GCNNNNNNNGC 5 cut(s) 434, 642, 983, 1166, 1187
BstNSI RCATGY 2 cut(s) 696, 1242
BstSCI CCNGG 5 cut(s) 362, 423, 428, 1108, 1483
BstSLI GKGCMC 2 cut(s) 485, 967
BstUI CGCG 4 cut(s) 343, 1003, 1144, 1160
BstV1I GCAGC 6 cut(s) 17, 453, 788, 973, 1134, 1202
BstX2I RGATCY 2 cut(s) 394, 1045
BstYI RGATCY 2 cut(s) 394, 1045
BstZ17I GTATAC 2 cut(s) 1164, 1339
BstZI CGGCCG 1 cut(s) 837
BsuI GTATCC 1 cut(s) 1284
BsuRI GGCC 7 cut(s) 271, 413, 428, 602, 761, 807, 839
BtgZI GCGATG 1 cut(s) 1208
BtsCI GGATG 3 cut(s) 242, 243, 1183
BtsIMutI CAGTG 2 cut(s) 732, 976
BveI ACCTGC 2 cut(s) 215, 1354
Cac8I GCNNGC 6 cut(s) 198, 345, 439, 454, 759, 937
CfoI GCGC 1 cut(s) 343
Cfr10I RCCGGY 3 cut(s) 65, 839, 944
Cfr13I GGNCC 2 cut(s) 269, 427
CseI GACGC 2 cut(s) 634, 1331
Csp6I GTAC 3 cut(s) 257, 712, 898
CspAI ACCGGT 2 cut(s) 65, 944
CviAII CATG 5 cut(s) 266, 273, 463, 693, 1239
CviQI GTAC 3 cut(s) 257, 712, 898
DdeI CTNAG 7 cut(s) 83, 398, 653, 682, 1027, 1250, 1425
DpnI GATC 4 cut(s) 396, 594, 1047, 1118
DpnII GATC 4 cut(s) 394, 592, 1045, 1116
DraIII CACNNNGTG 2 cut(s) 642, 971
EaeI YGGCCR 2 cut(s) 805, 837
EagI CGGCCG 1 cut(s) 837
Eam1104I CTCTTC 2 cut(s) 43, 610
EarI CTCTTC 2 cut(s) 43, 610
EclXI CGGCCG 1 cut(s) 837
Eco130I CCWWGG 1 cut(s) 120
Eco24I GRGCYC 1 cut(s) 585
Eco31I GGTCTC 1 cut(s) 857
Eco52I CGGCCG 1 cut(s) 837
Eco88I CYCGRG 1 cut(s) 1021
EcoT14I CCWWGG 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 585
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 5 cut(s) 269, 276, 466, 696, 1242
FalI AAGNNNNNCTT 2 cut(s) 746, 778
FaqI GGGAC 2 cut(s) 407, 1126
FatI CATG 5 cut(s) 265, 272, 462, 692, 1238
FauI CCCGC 2 cut(s) 1167, 1210
FblI GTMKAC 2 cut(s) 1163, 1338
Fnu4HI GCNGC 9 cut(s) 6, 442, 802, 805, 904, 987, 1145, 1148, 1191
FokI GGATG 3 cut(s) 229, 250, 1190
FriOI GRGCYC 1 cut(s) 585
Fsp4HI GCNGC 9 cut(s) 6, 442, 802, 805, 904, 987, 1145, 1148, 1191
FspBI CTAG 3 cut(s) 18, 1043, 1232
GlaI GCGC 1 cut(s) 342
GluI GCNGC 9 cut(s) 6, 442, 802, 805, 904, 987, 1145, 1148, 1191
GsaI CCCAGC 1 cut(s) 1386
HaeIII GGCC 7 cut(s) 271, 413, 428, 602, 761, 807, 839
HgaI GACGC 2 cut(s) 634, 1331
HhaI GCGC 1 cut(s) 343
Hin1II CATG 5 cut(s) 269, 276, 466, 696, 1242
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HinfI GANTC 8 cut(s) 91, 134, 147, 310, 538, 719, 1005, 1124
HphI GGTGA 3 cut(s) 55, 478, 1279
Hpy166II GTNNAC 6 cut(s) 259, 845, 965, 1155, 1164, 1339
Hpy188I TCNGA 3 cut(s) 409, 559, 1278
Hpy188III TCNNGA 3 cut(s) 655, 1128, 1417
Hpy8I GTNNAC 6 cut(s) 259, 845, 965, 1155, 1164, 1339
Hpy99I CGWCG 5 cut(s) 779, 833, 881, 1108, 1304
HpyAV CCTTC 2 cut(s) 122, 161
HpyCH4III ACNGT 6 cut(s) 421, 472, 920, 1135, 1267, 1352
HpyCH4IV ACGT 6 cut(s) 621, 777, 816, 1271, 1310, 1455
HpyCH4V TGCA 3 cut(s) 210, 452, 965
HpyF10VI GCNNNNNNNGC 5 cut(s) 434, 642, 983, 1166, 1187
HpyF3I CTNAG 7 cut(s) 83, 398, 653, 682, 1027, 1250, 1425
HpySE526I ACGT 6 cut(s) 621, 777, 816, 1271, 1310, 1455
Hsp92II CATG 5 cut(s) 269, 276, 466, 696, 1242
HspAI GCGC 1 cut(s) 341
Kpn2I TCCGGA 1 cut(s) 1127
Kzo9I GATC 4 cut(s) 394, 592, 1045, 1116
LmnI GCTCC 5 cut(s) 26, 580, 944, 974, 1486
Lsp1109I GCAGC 6 cut(s) 17, 453, 788, 973, 1134, 1202
LweI GCATC 4 cut(s) 251, 1065, 1168, 1384
MaeI CTAG 3 cut(s) 18, 1043, 1232
MaeII ACGT 6 cut(s) 621, 777, 816, 1271, 1310, 1455
MaeIII GTNAC 5 cut(s) 466, 629, 947, 1267, 1306
MalI GATC 4 cut(s) 396, 594, 1047, 1118
MbiI CCGCTC 1 cut(s) 186
MboI GATC 4 cut(s) 394, 592, 1045, 1116
MboII GAAGA 6 cut(s) 30, 597, 784, 787, 901, 1444
MflI RGATCY 2 cut(s) 394, 1045
MhlI GDGCHC 3 cut(s) 485, 585, 967
MluCI AATT 9 cut(s) 321, 377, 560, 668, 1083, 1226, 1286, 1316, 1332
MlyI GAGTC 5 cut(s) 85, 141, 532, 713, 1118
MmeI TCCRAC 4 cut(s) 673, 1286, 1293, 1453
MroI TCCGGA 1 cut(s) 1127
MseI TTAA 1 cut(s) 764
MslI CAYNNNNRTG 2 cut(s) 264, 1061
MspR9I CCNGG 5 cut(s) 364, 425, 430, 1110, 1485
MvnI CGCG 4 cut(s) 343, 1003, 1144, 1160
MwoI GCNNNNNNNGC 5 cut(s) 434, 642, 983, 1166, 1187
NciI CCSGG 5 cut(s) 364, 425, 430, 1110, 1485
NdeII GATC 4 cut(s) 394, 592, 1045, 1116
NlaIII CATG 5 cut(s) 269, 276, 466, 696, 1242
NlaIV GGNNCC 2 cut(s) 582, 1132
NmeAIII GCCGAG 2 cut(s) 578, 815
NmuCI GTSAC 2 cut(s) 466, 1267
NspI RCATGY 2 cut(s) 696, 1242
PaeR7I CTCGAG 1 cut(s) 1021
PaqCI CACCTGC 1 cut(s) 215
PcsI WCGNNNNNNNCGW 4 cut(s) 780, 1100, 1112, 1148
PfeI GAWTC 3 cut(s) 134, 310, 1005
PfoI TCCNGGA 1 cut(s) 362
PinAI ACCGGT 2 cut(s) 65, 944
PkrI GCNGC 9 cut(s) 7, 443, 803, 806, 905, 988, 1146, 1149, 1192
PleI GAGTC 5 cut(s) 85, 141, 532, 713, 1118
PpsI GAGTC 5 cut(s) 85, 141, 532, 713, 1118
Psp1406I AACGTT 1 cut(s) 621
PspFI CCCAGC 1 cut(s) 1382
PspN4I GGNNCC 2 cut(s) 582, 1132
PspPI GGNCC 2 cut(s) 269, 427
PspXI VCTCGAGB 1 cut(s) 1021
PsuI RGATCY 2 cut(s) 394, 1045
RsaI GTAC 3 cut(s) 258, 713, 899
RsaNI GTAC 3 cut(s) 257, 712, 898
RseI CAYNNNNRTG 2 cut(s) 264, 1061
SaqAI TTAA 1 cut(s) 764
SatI GCNGC 9 cut(s) 6, 442, 802, 805, 904, 987, 1145, 1148, 1191
Sau3AI GATC 4 cut(s) 394, 592, 1045, 1116
Sau96I GGNCC 2 cut(s) 269, 427
SchI GAGTC 5 cut(s) 85, 141, 532, 713, 1118
ScrFI CCNGG 5 cut(s) 364, 425, 430, 1110, 1485
SduI GDGCHC 3 cut(s) 485, 585, 967
SfaNI GCATC 4 cut(s) 251, 1065, 1168, 1384
Sfr274I CTCGAG 1 cut(s) 1021
SlaI CTCGAG 1 cut(s) 1021
SmiMI CAYNNNNRTG 2 cut(s) 264, 1061
SmlI CTYRAG 1 cut(s) 1021
SmoI CTYRAG 1 cut(s) 1021
Sse9I AATT 9 cut(s) 321, 377, 560, 668, 1083, 1226, 1286, 1316, 1332
SspMI CTAG 3 cut(s) 18, 1043, 1232
StyD4I CCNGG 5 cut(s) 362, 423, 428, 1108, 1483
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 6 cut(s) 421, 472, 920, 1135, 1267, 1352
TaiI ACGT 6 cut(s) 624, 780, 819, 1274, 1313, 1458
TaqI TCGA 5 cut(s) 591, 783, 879, 1022, 1122
TasI AATT 9 cut(s) 321, 377, 560, 668, 1083, 1226, 1286, 1316, 1332
TatI WGTACW 1 cut(s) 897
TauI GCSGC 3 cut(s) 807, 906, 1147
TfiI GAWTC 3 cut(s) 134, 310, 1005
Tru1I TTAA 1 cut(s) 764
Tru9I TTAA 1 cut(s) 764
TscAI CASTG 2 cut(s) 739, 976
TseFI GTSAC 2 cut(s) 466, 1267
TseI GCWGC 6 cut(s) 5, 441, 801, 986, 1147, 1190
Tsp45I GTSAC 2 cut(s) 466, 1267
TspDTI ATGAA 2 cut(s) 143, 323
TspGWI ACGGA 4 cut(s) 680, 694, 729, 1197
TspRI CASTG 2 cut(s) 739, 976
VneI GTGCAC 1 cut(s) 963
XapI RAATTY 4 cut(s) 560, 668, 1226, 1286
XceI RCATGY 2 cut(s) 696, 1242
XcmI CCANNNNNNNNNTGG 1 cut(s) 754
XhoI CTCGAG 1 cut(s) 1021
XmiI GTMKAC 2 cut(s) 1163, 1338
XspI CTAG 3 cut(s) 18, 1043, 1232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.