pycom15g04100

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000845
Physical Location & Seq
Forward (+)
2485447 .. 2488611
3165 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g04100.1

Sequence Viewer

Length: 1521 bp
ATGGCGGCAACTCCCAGTTTTTCTAGCTCCTCCTCCTCCTCCGCCTCCTCCTCCCTCATGAGCTATTTGCTTGGCTTTTCTGTATTTCTAACCCTATTTCTCAGCTCTTTGGAGAAGGGCTTTGCCATGGAAGAAAACCAGCTTCTCCATCAACATGCTCATACAGTTGAACTCAATTCTCTGCTGCCGGCAACCTCCTGCAGCCCTAGCTCCTCCGCCAAAGGTCACGACAACAAGGGACCAACGTCATCATCGACCGTACTGAAAGTGGTACACAAGCACGGACCATGCTCTGGAATCAAGAAGAAGAACTCCAAAATTCCAACCCACGCTCAAATCCTCACGCAAGACCAAGCCCGAGTTGATTCAATCCACTCCCGAGTCAACACTAAGAAAGCACCATCCGCAGCCACCGTCGACAAACTCCGCCCATCCGCCGAAACCAAAATTCCGGCCCAGTCCGGAAGCGTAGTGGGCTCAGGCAACTACATTGTCAGCGTGGGCCTGGGCTCGCCCAAGAAGATGCTCAGCCTCATCTTCGACACGGGAAGTGATCTGACGTGGACCCAATGCCGCCCATGTGCCAAATCATGTTACAAACAGAAGGAGCCCATCTTCGACCCCGCAGCATCCGCCTCATACGTCAACATCTCTTGTAGCTCCGCCACGTGTTCCGAACTGGAGTCAGGCACTGGAAACACTCCCCGCTGCTCGTCCTCCACATGCGTCTACGTCATTCAGTACGGAGACCAGTCATTCTCCGCCGGATTTTTCGGGAAGGAACGGCTGAGCTTAACGTCGACGGATATTTTTGACGGGTTTTTGTTCGGGTGCGGCCAGAACAATCAGGGACTCTTCGGGGGCGCCGCCGGTTTGCTCGGTCTCGGCCGGGATAAAATCTCCATCATCGAGCAATCCGCCTTGAAATACAACCGGTTCTTCTCCTACTGCCTCCCCTCCAAATCCAGCGGCACTGGATACCTCAGCTTCGGCAAAGGCGGTGGATCTTCCAGCGCCGTGAAATTCACACCCCTCGCCGACGTCTCCCAAGGCGGGTCGTTTTACGGCCTCGACGTCGTCGGGATCAGCGTTGGCGGGAAAAAATTACCGATTCCCGGTACAATTTTTTCCTCCGCGGGGACGATTATAGATTCCGGGACAGTGATCACGCGCCTGCCGCCGACAGCATACACGGCACTGCGCGACGCGTTTCAGCAGGGGATGAAGAGCTACCCGTCAGCTCCGGCGCTTTCGATTCTCGACACTTGTTACGACTTCAGTAAGGTCGATACGGTGACGTTTCCGAAAATTTCGTTTGCGTTCGGCGGCGGAACGACGTTGGAATTGGACCCGACCGGGATATTCTACGCGGCAAGTGCCGATCAAGTTTGCCTGGCGTTTGCCGCAAACGGTGACGACAGCGACATAGGGATATTTGGGAACGTTCAGCAGAAGAGGGTGCAGGTGGTTTATGATGTGGCTGGTGGAAAAATCGGATTTGCCCCTGCAGGATGCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

507

Amino Acids

52.81

Weight (kDa)

8.63

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 162 - 503 7.8e-24 Eukaryotic aspartyl protease
TAXi_N PF14543 163 - 332 1.1e-53 Xylanase inhibitor N-terminal
TAXi_C PF14541 355 - 501 1.1e-30 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1453
AatII GACGTC 2 cut(s) 1044, 1077
Acc36I ACCTGC 1 cut(s) 1453
AccB1I GGYRCC 1 cut(s) 863
AccB7I CCANNNNNTGG 1 cut(s) 293
AccI GTMKAC 3 cut(s) 417, 729, 800
AccII CGCG 5 cut(s) 1136, 1171, 1203, 1208, 1370
AccIII TCCGGA 1 cut(s) 461
AclI AACGTT 1 cut(s) 1443
AclWI GGATC 2 cut(s) 1012, 1091
AcoI YGGCCR 2 cut(s) 835, 886
AcsI RAATTY 4 cut(s) 318, 447, 1022, 1308
AcuI CTGAAG 1 cut(s) 1261
AcvI CACGTG 1 cut(s) 669
AcyI GRCGYC 3 cut(s) 864, 1041, 1074
AfaI GTAC 4 cut(s) 261, 273, 743, 1120
AfiI CCNNNNNNNGG 4 cut(s) 293, 1053, 1115, 1137
AflIII ACRYGT 2 cut(s) 668, 1206
AgeI ACCGGT 1 cut(s) 933
AgsI TTSAA 3 cut(s) 170, 369, 925
AjiI CACGTC 1 cut(s) 561
AjnI CCWGG 2 cut(s) 504, 1392
Alw26I GTCTC 3 cut(s) 741, 887, 1048
AlwI GGATC 2 cut(s) 1012, 1091
AlwNI CAGNNNCTG 1 cut(s) 692
Ama87I CYCGRG 2 cut(s) 357, 378
Aor13HI TCCGGA 1 cut(s) 461
AoxI GGCC 5 cut(s) 453, 502, 835, 886, 1066
ApeKI GCWGC 5 cut(s) 184, 201, 407, 626, 708
ApoI RAATTY 4 cut(s) 318, 447, 1022, 1308
AsiGI ACCGGT 1 cut(s) 933
AspLEI GCGC 5 cut(s) 866, 1016, 1173, 1203, 1249
AspS9I GGNCC 6 cut(s) 239, 284, 454, 502, 564, 1348
AsuC2I CCSGG 4 cut(s) 890, 1116, 1156, 1357
AsuHPI GGTGA 2 cut(s) 1306, 1424
AvaI CYCGRG 2 cut(s) 357, 378
AvaII GGWCC 4 cut(s) 239, 284, 564, 1348
BanI GGYRCC 1 cut(s) 863
BanII GRGCYC 3 cut(s) 479, 512, 612
BbrPI CACGTG 1 cut(s) 669
BbvCI CCTCAGC 1 cut(s) 983
BbvI GCAGC 5 cut(s) 171, 213, 419, 638, 695
BccI CCATC 5 cut(s) 156, 409, 439, 620, 911
BceAI ACGGC 4 cut(s) 800, 1001, 1081, 1209
BciT130I CCWGG 2 cut(s) 506, 1394
BciVI GTATCC 1 cut(s) 971
BclI TGATCA 1 cut(s) 1164
BcnI CCSGG 4 cut(s) 890, 1116, 1156, 1357
BcoDI GTCTC 3 cut(s) 741, 887, 1048
BfaI CTAG 2 cut(s) 24, 207
BfmI CTRYAG 2 cut(s) 199, 1506
BfoI RGCGCY 3 cut(s) 867, 1017, 1250
BfuAI ACCTGC 1 cut(s) 1453
BfuI GTATCC 1 cut(s) 971
BlpI GCTNAGC 2 cut(s) 527, 788
Bme1390I CCNGG 6 cut(s) 506, 890, 1116, 1156, 1357, 1394
Bme18I GGWCC 4 cut(s) 239, 284, 564, 1348
BmeT110I CYCGRG 2 cut(s) 357, 378
BmgBI CACGTC 1 cut(s) 561
BmgT120I GGNCC 6 cut(s) 239, 284, 454, 502, 564, 1348
BmiI GGNNCC 5 cut(s) 240, 566, 609, 865, 1350
BmrFI CCNGG 6 cut(s) 506, 890, 1116, 1156, 1357, 1394
BmrI ACTGGG 2 cut(s) 9, 451
BmsI GCATC 3 cut(s) 513, 638, 1502
BmuI ACTGGG 2 cut(s) 9, 451
BoxI GACNNNNGTC 1 cut(s) 244
BpmI CTGGAG 1 cut(s) 701
Bpu10I CCTNAGC 2 cut(s) 478, 983
Bpu1102I GCTNAGC 2 cut(s) 527, 788
BpuMI CCSGG 4 cut(s) 890, 1116, 1156, 1357
BsaAI YACGTR 1 cut(s) 669
BsaHI GRCGYC 3 cut(s) 864, 1041, 1074
BsaI GGTCTC 2 cut(s) 741, 887
BsaJI CCNNGG 4 cut(s) 126, 505, 1048, 1134
BsaWI WCCGGW 2 cut(s) 461, 933
Bsc4I CCNNNNNNNGG 4 cut(s) 293, 1053, 1115, 1137
Bse118I RCCGGY 3 cut(s) 187, 869, 933
Bse1I ACTGG 6 cut(s) 15, 457, 684, 697, 751, 979
BseAI TCCGGA 1 cut(s) 461
BseBI CCWGG 2 cut(s) 506, 1394
BseDI CCNNGG 4 cut(s) 126, 505, 1048, 1134
BseGI GGATG 5 cut(s) 401, 431, 629, 1227, 1517
BseLI CCNNNNNNNGG 4 cut(s) 293, 1053, 1115, 1137
BseMII CTCAG 5 cut(s) 115, 492, 541, 779, 997
BseNI ACTGG 6 cut(s) 15, 457, 684, 697, 751, 979
BseRI GAGGAG 7 cut(s) 19, 22, 25, 28, 37, 40, 202
BseX3I CGGCCG 1 cut(s) 886
BseXI GCAGC 5 cut(s) 171, 213, 419, 638, 695
BsgI GTGCAG 1 cut(s) 1481
Bsh1236I CGCG 5 cut(s) 1136, 1171, 1203, 1208, 1370
Bsh1285I CGRYCG 3 cut(s) 258, 889, 1356
BshFI GGCC 5 cut(s) 455, 504, 837, 888, 1068
BshNI GGYRCC 1 cut(s) 863
BshTI ACCGGT 1 cut(s) 933
BsiEI CGRYCG 3 cut(s) 258, 889, 1356
BsiHKCI CYCGRG 2 cut(s) 357, 378
BslFI GGGAC 4 cut(s) 252, 864, 1153, 1171
BslI CCNNNNNNNGG 4 cut(s) 293, 1053, 1115, 1137
BsmAI GTCTC 3 cut(s) 741, 887, 1048
BsmBI CGTCTC 1 cut(s) 1048
BsmFI GGGAC 4 cut(s) 252, 864, 1153, 1171
BsnI GGCC 5 cut(s) 455, 504, 837, 888, 1068
Bso31I GGTCTC 2 cut(s) 741, 887
BsoBI CYCGRG 2 cut(s) 357, 378
Bsp1286I GDGCHC 3 cut(s) 479, 512, 612
Bsp13I TCCGGA 1 cut(s) 461
Bsp143I GATC 5 cut(s) 553, 1004, 1083, 1164, 1381
Bsp1720I GCTNAGC 2 cut(s) 527, 788
Bsp19I CCATGG 1 cut(s) 126
BspANI GGCC 5 cut(s) 455, 504, 837, 888, 1068
BspCNI CTCAG 5 cut(s) 114, 491, 540, 780, 996
BspEI TCCGGA 1 cut(s) 461
BspFNI CGCG 5 cut(s) 1136, 1171, 1203, 1208, 1370
BspHI TCATGA 1 cut(s) 57
BspLI GGNNCC 5 cut(s) 240, 566, 609, 865, 1350
BspMAI CTGCAG 2 cut(s) 203, 1510
BspMI ACCTGC 1 cut(s) 1453
BspPI GGATC 2 cut(s) 1012, 1091
BspQI GCTCTTC 1 cut(s) 1220
BspT107I GGYRCC 1 cut(s) 863
BspTNI GGTCTC 2 cut(s) 741, 887
BsrFI RCCGGY 3 cut(s) 187, 869, 933
BsrI ACTGG 6 cut(s) 15, 457, 684, 697, 751, 979
BssAI RCCGGY 3 cut(s) 187, 869, 933
BssECI CCNNGG 4 cut(s) 126, 505, 1048, 1134
BssMI GATC 5 cut(s) 553, 1004, 1083, 1164, 1381
BssNI GRCGYC 3 cut(s) 864, 1041, 1074
BssT1I CCWWGG 2 cut(s) 126, 1048
Bst2UI CCWGG 2 cut(s) 506, 1394
Bst4CI ACNGT 6 cut(s) 166, 259, 415, 1162, 1294, 1412
Bst6I CTCTTC 3 cut(s) 860, 1220, 1448
BstACI GRCGYC 3 cut(s) 864, 1041, 1074
BstBAI YACGTR 1 cut(s) 669
BstC8I GCNNGC 3 cut(s) 189, 512, 1175
BstDEI CTNAG 6 cut(s) 101, 390, 478, 527, 788, 983
BstDSI CCRYGG 2 cut(s) 126, 1134
BstF5I GGATG 5 cut(s) 401, 431, 629, 1227, 1517
BstFNI CGCG 5 cut(s) 1136, 1171, 1203, 1208, 1370
BstH2I RGCGCY 3 cut(s) 867, 1017, 1250
BstHHI GCGC 5 cut(s) 866, 1016, 1173, 1203, 1249
BstKTI GATC 5 cut(s) 556, 1007, 1086, 1167, 1384
BstMAI GTCTC 3 cut(s) 741, 887, 1048
BstMBI GATC 5 cut(s) 553, 1004, 1083, 1164, 1381
BstMCI CGRYCG 3 cut(s) 258, 889, 1356
BstMWI GCNNNNNNNGC 8 cut(s) 207, 404, 474, 632, 1177, 1193, 1376, 1403
BstNI CCWGG 2 cut(s) 506, 1394
BstNSI RCATGY 2 cut(s) 158, 726
BstPAI GACNNNNGTC 1 cut(s) 244
BstSCI CCNGG 6 cut(s) 504, 888, 1114, 1154, 1355, 1392
BstSFI CTRYAG 2 cut(s) 199, 1506
BstUI CGCG 5 cut(s) 1136, 1171, 1203, 1208, 1370
BstV1I GCAGC 5 cut(s) 171, 213, 419, 638, 695
BstX2I RGATCY 1 cut(s) 1004
BstYI RGATCY 1 cut(s) 1004
BstZI CGGCCG 1 cut(s) 886
BsuI GTATCC 1 cut(s) 971
BsuRI GGCC 5 cut(s) 455, 504, 837, 888, 1068
BtgI CCRYGG 2 cut(s) 126, 1134
BtrI CACGTC 1 cut(s) 561
BtsCI GGATG 5 cut(s) 401, 431, 629, 1227, 1517
BtsI GCAGTG 1 cut(s) 1196
BtsIMutI CAGTG 4 cut(s) 690, 972, 1167, 1196
BveI ACCTGC 1 cut(s) 1453
Cac8I GCNNGC 3 cut(s) 189, 512, 1175
CaiI CAGNNNCTG 1 cut(s) 692
CciI TCATGA 1 cut(s) 57
CfoI GCGC 5 cut(s) 866, 1016, 1173, 1203, 1249
Cfr10I RCCGGY 3 cut(s) 187, 869, 933
Cfr13I GGNCC 6 cut(s) 239, 284, 454, 502, 564, 1348
Cfr42I CCGCGG 1 cut(s) 1137
CseI GACGC 2 cut(s) 715, 1214
Csp6I GTAC 4 cut(s) 260, 272, 742, 1119
CspAI ACCGGT 1 cut(s) 933
CspCI CAANNNNNGTGG 2 cut(s) 982, 1017
CviAII CATG 7 cut(s) 58, 127, 155, 288, 579, 591, 723
CviQI GTAC 4 cut(s) 260, 272, 742, 1119
DdeI CTNAG 6 cut(s) 101, 390, 478, 527, 788, 983
DinI GGCGCC 1 cut(s) 865
DpnI GATC 5 cut(s) 555, 1006, 1085, 1166, 1383
DpnII GATC 5 cut(s) 553, 1004, 1083, 1164, 1381
EaeI YGGCCR 2 cut(s) 835, 886
EagI CGGCCG 1 cut(s) 886
Eam1104I CTCTTC 3 cut(s) 860, 1220, 1448
EarI CTCTTC 3 cut(s) 860, 1220, 1448
EciI GGCGGA 9 cut(s) 31, 205, 416, 424, 622, 652, 751, 907, 1344
EclXI CGGCCG 1 cut(s) 886
Eco130I CCWWGG 2 cut(s) 126, 1048
Eco24I GRGCYC 3 cut(s) 479, 512, 612
Eco31I GGTCTC 2 cut(s) 741, 887
Eco47I GGWCC 4 cut(s) 239, 284, 564, 1348
Eco52I CGGCCG 1 cut(s) 886
Eco57I CTGAAG 1 cut(s) 1261
Eco72I CACGTG 1 cut(s) 669
Eco88I CYCGRG 2 cut(s) 357, 378
EcoRII CCWGG 2 cut(s) 504, 1392
EcoT14I CCWWGG 2 cut(s) 126, 1048
EcoT38I GRGCYC 3 cut(s) 479, 512, 612
EgeI GGCGCC 1 cut(s) 865
EheI GGCGCC 1 cut(s) 865
ErhI CCWWGG 2 cut(s) 126, 1048
Esp3I CGTCTC 1 cut(s) 1048
FaeI CATG 7 cut(s) 61, 130, 158, 291, 582, 594, 726
FaqI GGGAC 4 cut(s) 252, 864, 1153, 1171
FatI CATG 7 cut(s) 57, 126, 154, 287, 578, 590, 722
FauI CCCGC 5 cut(s) 631, 713, 1046, 1088, 1129
FbaI TGATCA 1 cut(s) 1164
FblI GTMKAC 3 cut(s) 417, 729, 800
FokI GGATG 4 cut(s) 388, 418, 616, 1234
FriOI GRGCYC 3 cut(s) 479, 512, 612
FspBI CTAG 2 cut(s) 24, 207
GlaI GCGC 5 cut(s) 865, 1015, 1172, 1202, 1248
GsuI CTGGAG 1 cut(s) 701
HaeII RGCGCY 3 cut(s) 867, 1017, 1250
HaeIII GGCC 5 cut(s) 455, 504, 837, 888, 1068
HgaI GACGC 2 cut(s) 715, 1214
HhaI GCGC 5 cut(s) 866, 1016, 1173, 1203, 1249
Hin1I GRCGYC 3 cut(s) 864, 1041, 1074
Hin1II CATG 7 cut(s) 61, 130, 158, 291, 582, 594, 726
Hin6I GCGC 5 cut(s) 864, 1014, 1171, 1201, 1247
HinP1I GCGC 5 cut(s) 864, 1014, 1171, 1201, 1247
HincII GTYRAC 4 cut(s) 385, 418, 646, 801
HindII GTYRAC 4 cut(s) 385, 418, 646, 801
HinfI GANTC 8 cut(s) 297, 365, 381, 683, 852, 1111, 1151, 1255
HphI GGTGA 2 cut(s) 1306, 1424
Hpy166II GTNNAC 7 cut(s) 274, 385, 418, 564, 646, 730, 801
Hpy188I TCNGA 4 cut(s) 558, 676, 1305, 1496
Hpy188III TCNNGA 9 cut(s) 58, 227, 294, 301, 378, 462, 775, 1081, 1259
Hpy8I GTNNAC 7 cut(s) 274, 385, 418, 564, 646, 730, 801
Hpy99I CGWCG 9 cut(s) 419, 802, 805, 1043, 1076, 1079, 1082, 1208, 1339
HpyAV CCTTC 3 cut(s) 109, 598, 772
HpyCH4III ACNGT 6 cut(s) 166, 259, 415, 1162, 1294, 1412
HpyCH4V TGCA 3 cut(s) 201, 1462, 1508
HpyF10VI GCNNNNNNNGC 8 cut(s) 207, 404, 474, 632, 1177, 1193, 1376, 1403
HpyF3I CTNAG 6 cut(s) 101, 390, 478, 527, 788, 983
Hsp92I GRCGYC 3 cut(s) 864, 1041, 1074
Hsp92II CATG 7 cut(s) 61, 130, 158, 291, 582, 594, 726
HspAI GCGC 5 cut(s) 864, 1014, 1171, 1201, 1247
KasI GGCGCC 1 cut(s) 863
Kpn2I TCCGGA 1 cut(s) 461
KroI GCCGGC 1 cut(s) 187
KroNI GCCGGC 1 cut(s) 189
Ksp22I TGATCA 1 cut(s) 1164
KspI CCGCGG 1 cut(s) 1137
Kzo9I GATC 5 cut(s) 553, 1004, 1083, 1164, 1381
LguI GCTCTTC 1 cut(s) 1220
LmnI GCTCC 5 cut(s) 32, 215, 607, 665, 1246
Lsp1109I GCAGC 5 cut(s) 171, 213, 419, 638, 695
LweI GCATC 3 cut(s) 513, 638, 1502
MaeI CTAG 2 cut(s) 24, 207
MaeIII GTNAC 5 cut(s) 224, 593, 1268, 1294, 1412
MalI GATC 5 cut(s) 555, 1006, 1085, 1166, 1383
MboI GATC 5 cut(s) 553, 1004, 1083, 1164, 1381
MflI RGATCY 1 cut(s) 1004
MhlI GDGCHC 3 cut(s) 479, 512, 612
MluCI AATT 8 cut(s) 175, 318, 447, 1022, 1103, 1122, 1308, 1343
MluI ACGCGT 1 cut(s) 1206
Mly113I GGCGCC 1 cut(s) 864
MlyI GAGTC 3 cut(s) 390, 692, 846
MmeI TCCRAC 2 cut(s) 347, 1320
MroI TCCGGA 1 cut(s) 461
MroNI GCCGGC 1 cut(s) 187
MseI TTAA 1 cut(s) 794
MslI CAYNNNNRTG 1 cut(s) 153
MspA1I CMGCKG 3 cut(s) 708, 969, 1136
MspR9I CCNGG 6 cut(s) 506, 890, 1116, 1156, 1357, 1394
MvaI CCWGG 2 cut(s) 506, 1394
MvnI CGCG 5 cut(s) 1136, 1171, 1203, 1208, 1370
MwoI GCNNNNNNNGC 8 cut(s) 207, 404, 474, 632, 1177, 1193, 1376, 1403
NaeI GCCGGC 1 cut(s) 189
NarI GGCGCC 1 cut(s) 864
NciI CCSGG 4 cut(s) 890, 1116, 1156, 1357
NcoI CCATGG 1 cut(s) 126
NdeII GATC 5 cut(s) 553, 1004, 1083, 1164, 1381
NgoMIV GCCGGC 1 cut(s) 187
NlaIII CATG 7 cut(s) 61, 130, 158, 291, 582, 594, 726
NlaIV GGNNCC 5 cut(s) 240, 566, 609, 865, 1350
NmeAIII GCCGAG 1 cut(s) 864
NmuCI GTSAC 3 cut(s) 224, 1294, 1412
NspI RCATGY 2 cut(s) 158, 726
PagI TCATGA 1 cut(s) 57
PaqCI CACCTGC 1 cut(s) 1453
PciSI GCTCTTC 1 cut(s) 1220
PcsI WCGNNNNNNNCGW 3 cut(s) 251, 1071, 1086
PdiI GCCGGC 1 cut(s) 189
PfeI GAWTC 5 cut(s) 297, 365, 1111, 1151, 1255
PflFI GACNNNGTC 1 cut(s) 1076
PflMI CCANNNNNTGG 1 cut(s) 293
PfoI TCCNGGA 1 cut(s) 1154
PinAI ACCGGT 1 cut(s) 933
PleI GAGTC 3 cut(s) 389, 691, 846
PluTI GGCGCC 1 cut(s) 867
PmaCI CACGTG 1 cut(s) 669
PmlI CACGTG 1 cut(s) 669
PpsI GAGTC 3 cut(s) 389, 691, 846
Ppu21I YACGTR 1 cut(s) 669
PshAI GACNNNNGTC 1 cut(s) 244
Psp1406I AACGTT 1 cut(s) 1443
Psp6I CCWGG 2 cut(s) 504, 1392
PspCI CACGTG 1 cut(s) 669
PspGI CCWGG 2 cut(s) 504, 1392
PspN4I GGNNCC 5 cut(s) 240, 566, 609, 865, 1350
PspPI GGNCC 6 cut(s) 239, 284, 454, 502, 564, 1348
PstI CTGCAG 2 cut(s) 203, 1510
PstNI CAGNNNCTG 1 cut(s) 692
PsuI RGATCY 1 cut(s) 1004
PsyI GACNNNGTC 1 cut(s) 1076
RsaI GTAC 4 cut(s) 261, 273, 743, 1120
RsaNI GTAC 4 cut(s) 260, 272, 742, 1119
RseI CAYNNNNRTG 1 cut(s) 153
SacII CCGCGG 1 cut(s) 1137
SalI GTCGAC 2 cut(s) 416, 799
SapI GCTCTTC 1 cut(s) 1220
SaqAI TTAA 1 cut(s) 794
Sau3AI GATC 5 cut(s) 553, 1004, 1083, 1164, 1381
Sau96I GGNCC 6 cut(s) 239, 284, 454, 502, 564, 1348
SbfI CCTGCAGG 1 cut(s) 1510
SchI GAGTC 3 cut(s) 390, 692, 846
ScrFI CCNGG 6 cut(s) 506, 890, 1116, 1156, 1357, 1394
SdaI CCTGCAGG 1 cut(s) 1510
SduI GDGCHC 3 cut(s) 479, 512, 612
SfaNI GCATC 3 cut(s) 513, 638, 1502
SfcI CTRYAG 2 cut(s) 199, 1506
SfoI GGCGCC 1 cut(s) 865
Sfr303I CCGCGG 1 cut(s) 1137
SgrBI CCGCGG 1 cut(s) 1137
SgrDI CGTCGACG 1 cut(s) 799
SinI GGWCC 4 cut(s) 239, 284, 564, 1348
SmiMI CAYNNNNRTG 1 cut(s) 153
Sse8387I CCTGCAGG 1 cut(s) 1510
Sse9I AATT 8 cut(s) 175, 318, 447, 1022, 1103, 1122, 1308, 1343
SspDI GGCGCC 1 cut(s) 863
SspMI CTAG 2 cut(s) 24, 207
StyD4I CCNGG 6 cut(s) 504, 888, 1114, 1154, 1355, 1392
StyI CCWWGG 2 cut(s) 126, 1048
TaaI ACNGT 6 cut(s) 166, 259, 415, 1162, 1294, 1412
TaqII GACCGA 1 cut(s) 869
TasI AATT 8 cut(s) 175, 318, 447, 1022, 1103, 1122, 1308, 1343
TauI GCSGC 9 cut(s) 8, 576, 837, 869, 972, 1180, 1329, 1373, 1406
TfiI GAWTC 5 cut(s) 297, 365, 1111, 1151, 1255
Tru1I TTAA 1 cut(s) 794
Tru9I TTAA 1 cut(s) 794
TscAI CASTG 4 cut(s) 697, 979, 1167, 1203
TseFI GTSAC 3 cut(s) 224, 1294, 1412
TseI GCWGC 5 cut(s) 184, 201, 407, 626, 708
Tsp45I GTSAC 3 cut(s) 224, 1294, 1412
TspDTI ATGAA 1 cut(s) 1238
TspGWI ACGGA 3 cut(s) 297, 759, 818
TspRI CASTG 4 cut(s) 697, 979, 1167, 1203
Tth111I GACNNNGTC 1 cut(s) 1076
Van91I CCANNNNNTGG 1 cut(s) 293
VpaK11BI GGWCC 4 cut(s) 239, 284, 564, 1348
XapI RAATTY 4 cut(s) 318, 447, 1022, 1308
XceI RCATGY 2 cut(s) 158, 726
XmiI GTMKAC 3 cut(s) 417, 729, 800
XspI CTAG 2 cut(s) 24, 207
ZraI GACGTC 2 cut(s) 1042, 1075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.