Rw1G023930

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000845
Physical Location & Seq
Reverse (-)
49975985 .. 49978739
2755 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G023930.1

Sequence Viewer

Length: 1455 bp
ATGGCAACTCCCAGTGTTCATTCTGGCTGCTCCTTCCTCGCCAACAAGTACTTGATTTCCTTTTGTGTATTTGTATGCCTTAATATTCTCTCTTCTTTGGACAAGGGCTTCGCTCATCAGACAGATGTTGAACTCAGCTCTCTACTACCAGCATCGACCTGCAGCCCCTCCACCAGAGGTCATGATAAGAAGAAGGCAACACTTAAAGTGGTACACAGGCATGGTCCATGCTCTAAGATGTCCCAGCCTAACGAATACAAGACCACAACTCCAACTCACACTCAGATCCTCCAGCAGGACCAAGCCCGAGTCACCTCAATCCACTCCCGGATGGGTGACAACGTAAGGCAAACCGACGCATCCATCCCCGCCAAGTCCGGCAGCGTAGTGGGCTCGGGAAACTACATTGTGACCGTGGGCTTGGGCTCACCCGCAAAACAACTCTTGCTCATATTTGACACCGGGAGCGACCTCACTTGGACTCAGTGTCAACCTTGTGTCAGGTCATGTTACAAACAGAAGGAGCCCATCTTTGACCCATCCCTCTCCAAATCGTATACCAACATAACCTGTAATTCGCCATTCTGCTCTCAACTCATATCAGCCACAGGTACCAGACCTTATTGCAGAAACGGCACATCAACTTGCGTATACAACATACAATACAGAGATAACTCCTTATCCGTGGGATTCTTTGCTAAAGAAAGGTTGACACTAACGTCAACGGACTATTTCGATGGGTTTCTCTTCGGCTGTGGCCAAAACAATCAAGGCCTTTTTGGCGGGTCTGCCGGGTTATTGGGCCTGGGCCCTAACAAAATCTCCTTTGTCGAACAGAGTGCCCAAAAGTACGGCCGCTTCTTCTCCTACTGCCTACCCTCAACTTCTTGCGCCACCGGTTACCTTAGCTTTGGAAAAGGCGGCGGACCCTCCAAAGCCGTCAAGTTCATGCCGCTCTCCATTGTATCTGGACGCGGGTACTTAGGAGGGTCCTTTTACGGCCTGGATGTCGTTGGAATCAACGTCAGCGGACGTCGGGTATCGATTCCTGCTTCGGTTTTTTCGTCCTCAGGGACCATCATTGACACAGGGACGATGATAACGCGACTTCCGGCAACGGCGTACAATGCTTTGAGGGATGAGTTTAAGCGAGGAATGAAAAGCTACCCGCAAGCTGAGGCGCTTTCGATACTGGACACGTGCTACGACCTGAGCGGGAGCAGCAAGGTGTCGTACCCCAAAATAGCGTTCGCTTTCAGCGGTGGATTGACATTGGATTTGGATGCGACAGGCATATTTTACGTGGCCAATGCTTCACAGGTGTGCTTGGCGTTCGCTGGGAATAGTGATGACAGCGACATTGCCATATTTGGGAATGTTCAACAGAAACGGTTGCAGGTAGTGTATGACGTCGCCGGTGGAAAGATTGGATTTGCCCCAGCTGGTTGCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

484

Amino Acids

51.68

Weight (kDa)

8.94

Isoelectric Point (pI)

37.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 134 - 481 2.5e-19 Eukaryotic aspartyl protease
TAXi_N PF14543 135 - 306 5e-52 Xylanase inhibitor N-terminal
TAXi_C PF14541 333 - 479 7.1e-29 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 718
AatII GACGTC 2 cut(s) 1036, 1413
Acc36I ACCTGC 2 cut(s) 167, 1387
Acc65I GGTACC 1 cut(s) 611
AccB1I GGYRCC 1 cut(s) 611
AccBSI CCGCTC 2 cut(s) 955, 1215
AccI GTMKAC 2 cut(s) 557, 651
AccII CGCG 2 cut(s) 975, 1105
AclWI GGATC 1 cut(s) 280
AcoI YGGCCR 3 cut(s) 757, 853, 1305
AcvI CACGTG 1 cut(s) 1200
AcyI GRCGYC 2 cut(s) 1033, 1410
AfaI GTAC 7 cut(s) 50, 213, 613, 851, 980, 1124, 1235
AfiI CCNNNNNNNGG 2 cut(s) 295, 1371
AflIII ACRYGT 1 cut(s) 1197
AgeI ACCGGT 1 cut(s) 896
AgsI TTSAA 2 cut(s) 131, 1382
AhdI GACNNNNNGTC 1 cut(s) 486
AjnI CCWGG 2 cut(s) 804, 1002
AjuI GAANNNNNNNTTGG 2 cut(s) 1232, 1264
AleI CACNNNNGTG 1 cut(s) 1321
AluBI AGCT 5 cut(s) 138, 909, 1164, 1175, 1442
AluI AGCT 5 cut(s) 138, 909, 1164, 1175, 1442
AlwI GGATC 1 cut(s) 280
Ama87I CYCGRG 2 cut(s) 306, 394
AoxI GGCC 7 cut(s) 757, 772, 802, 808, 853, 1000, 1305
ApaI GGGCCC 1 cut(s) 812
ApeKI GCWGC 4 cut(s) 27, 162, 381, 1221
ArsI GACNNNNNNTTYG 4 cut(s) 92, 124, 1261, 1293
AsiGI ACCGGT 1 cut(s) 896
Asp718I GGTACC 1 cut(s) 611
AspLEI GCGC 2 cut(s) 893, 1183
AspS9I GGNCC 8 cut(s) 224, 298, 802, 808, 809, 926, 990, 1074
AsuC2I CCSGG 3 cut(s) 328, 463, 793
AsuHPI GGTGA 3 cut(s) 304, 347, 420
AvaI CYCGRG 2 cut(s) 306, 394
AvaII GGWCC 5 cut(s) 224, 298, 926, 990, 1074
AxyI CCTNAGG 1 cut(s) 1069
BaeGI GKGCMC 2 cut(s) 812, 844
BalI TGGCCA 2 cut(s) 759, 1307
BanI GGYRCC 1 cut(s) 611
BanII GRGCYC 4 cut(s) 395, 428, 528, 812
BarI GAAGNNNNNNTAC 2 cut(s) 842, 874
BbrPI CACGTG 1 cut(s) 1200
BbvCI CCTCAGC 1 cut(s) 1176
BbvI GCAGC 4 cut(s) 14, 174, 393, 1233
BccI CCATC 6 cut(s) 325, 371, 536, 547, 731, 1085
BceAI ACGGC 5 cut(s) 649, 868, 923, 1015, 1134
BcgI CGANNNNNNTGC 2 cut(s) 821, 855
BciT130I CCWGG 2 cut(s) 806, 1004
BcnI CCSGG 3 cut(s) 328, 463, 793
BfmI CTRYAG 1 cut(s) 160
BfoI RGCGCY 1 cut(s) 1184
BfuAI ACCTGC 2 cut(s) 167, 1387
BglI GCCNNNNNGGC 1 cut(s) 780
BisI GCNGC 7 cut(s) 28, 163, 382, 856, 922, 953, 1222
BlsI GCNGC 7 cut(s) 29, 164, 383, 857, 923, 954, 1223
BmcAI AGTACT 1 cut(s) 50
Bme1390I CCNGG 5 cut(s) 328, 463, 793, 806, 1004
Bme18I GGWCC 5 cut(s) 224, 298, 926, 990, 1074
BmeRI GACNNNNNGTC 1 cut(s) 486
BmeT110I CYCGRG 2 cut(s) 306, 394
BmgT120I GGNCC 8 cut(s) 224, 298, 802, 808, 809, 926, 990, 1074
BmiI GGNNCC 6 cut(s) 525, 613, 810, 928, 991, 1075
BmrFI CCNGG 5 cut(s) 328, 463, 793, 806, 1004
BmrI ACTGGG 1 cut(s) 6
BmsI GCATC 3 cut(s) 161, 368, 1273
BmuI ACTGGG 1 cut(s) 6
BpmI CTGGAG 1 cut(s) 275
Bpu10I CCTNAGC 3 cut(s) 905, 1176, 1211
BpuMI CCSGG 3 cut(s) 328, 463, 793
Bsa29I ATCGAT 1 cut(s) 1043
BsaAI YACGTR 2 cut(s) 1200, 1303
BsaHI GRCGYC 2 cut(s) 1033, 1410
BsaJI CCNNGG 3 cut(s) 414, 684, 805
BsaWI WCCGGW 1 cut(s) 896
BsaXI ACNNNNNCTCC 4 cut(s) 253, 283, 806, 836
Bsc4I CCNNNNNNNGG 2 cut(s) 295, 1371
Bse118I RCCGGY 2 cut(s) 896, 1415
Bse1I ACTGG 2 cut(s) 12, 1197
Bse21I CCTNAGG 1 cut(s) 1069
Bse3DI GCAATG 1 cut(s) 1359
BseBI CCWGG 2 cut(s) 806, 1004
BseCI ATCGAT 1 cut(s) 1043
BseDI CCNNGG 3 cut(s) 414, 684, 805
BseGI GGATG 7 cut(s) 336, 359, 363, 539, 1012, 1144, 1288
BseLI CCNNNNNNNGG 2 cut(s) 295, 1371
BseMI GCAATG 1 cut(s) 1359
BseMII CTCAG 6 cut(s) 148, 296, 497, 1083, 1167, 1202
BseNI ACTGG 2 cut(s) 12, 1197
BseSI GKGCMC 2 cut(s) 812, 844
BseX3I CGGCCG 1 cut(s) 853
BseXI GCAGC 4 cut(s) 14, 174, 393, 1233
BseYI CCCAGC 3 cut(s) 243, 1337, 1438
Bsh1236I CGCG 2 cut(s) 975, 1105
Bsh1285I CGRYCG 1 cut(s) 856
BshFI GGCC 7 cut(s) 759, 774, 804, 810, 855, 1002, 1307
BshNI GGYRCC 1 cut(s) 611
BshTI ACCGGT 1 cut(s) 896
BshVI ATCGAT 1 cut(s) 1043
BsiEI CGRYCG 1 cut(s) 856
BsiHKCI CYCGRG 2 cut(s) 306, 394
BsiSI CCGG 7 cut(s) 328, 378, 462, 792, 897, 1112, 1416
BslFI GGGAC 3 cut(s) 226, 1087, 1105
BslI CCNNNNNNNGG 2 cut(s) 295, 1371
BsmFI GGGAC 3 cut(s) 226, 1087, 1105
BsnI GGCC 7 cut(s) 759, 774, 804, 810, 855, 1002, 1307
BsoBI CYCGRG 2 cut(s) 306, 394
Bsp120I GGGCCC 1 cut(s) 808
Bsp1286I GDGCHC 5 cut(s) 395, 428, 528, 812, 844
Bsp143I GATC 1 cut(s) 285
BspANI GGCC 7 cut(s) 759, 774, 804, 810, 855, 1002, 1307
BspCNI CTCAG 6 cut(s) 147, 295, 496, 1082, 1168, 1203
BspDI ATCGAT 1 cut(s) 1043
BspFNI CGCG 2 cut(s) 975, 1105
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 6 cut(s) 525, 613, 810, 928, 991, 1075
BspMAI CTGCAG 1 cut(s) 164
BspMI ACCTGC 2 cut(s) 167, 1387
BspPI GGATC 1 cut(s) 280
BspT107I GGYRCC 1 cut(s) 611
BsrBI CCGCTC 2 cut(s) 955, 1215
BsrDI GCAATG 1 cut(s) 1359
BsrFI RCCGGY 2 cut(s) 896, 1415
BsrI ACTGG 2 cut(s) 12, 1197
BssAI RCCGGY 2 cut(s) 896, 1415
BssECI CCNNGG 3 cut(s) 414, 684, 805
BssMI GATC 1 cut(s) 285
BssNAI GTATAC 2 cut(s) 558, 652
BssNI GRCGYC 2 cut(s) 1033, 1410
Bst1107I GTATAC 2 cut(s) 558, 652
Bst2UI CCWGG 2 cut(s) 806, 1004
Bst4CI ACNGT 2 cut(s) 415, 1392
Bst6I CTCTTC 2 cut(s) 97, 752
BstACI GRCGYC 2 cut(s) 1033, 1410
BstBAI YACGTR 2 cut(s) 1200, 1303
BstC8I GCNNGC 1 cut(s) 1173
BstDEI CTNAG 9 cut(s) 134, 234, 282, 483, 905, 982, 1069, 1176, 1211
BstDSI CCRYGG 2 cut(s) 414, 684
BstEII GGTNACC 1 cut(s) 899
BstENI CCTNNNNNAGG 1 cut(s) 293
BstF5I GGATG 7 cut(s) 336, 359, 363, 539, 1012, 1144, 1288
BstFNI CGCG 2 cut(s) 975, 1105
BstH2I RGCGCY 1 cut(s) 1184
BstHHI GCGC 2 cut(s) 893, 1183
BstKTI GATC 1 cut(s) 288
BstMBI GATC 1 cut(s) 285
BstMCI CGRYCG 1 cut(s) 856
BstMWI GCNNNNNNNGC 5 cut(s) 390, 633, 780, 1127, 1221
BstNI CCWGG 2 cut(s) 806, 1004
BstPI GGTNACC 1 cut(s) 899
BstSCI CCNGG 5 cut(s) 326, 461, 791, 804, 1002
BstSFI CTRYAG 1 cut(s) 160
BstSLI GKGCMC 2 cut(s) 812, 844
BstUI CGCG 2 cut(s) 975, 1105
BstV1I GCAGC 4 cut(s) 14, 174, 393, 1233
BstX2I RGATCY 1 cut(s) 285
BstYI RGATCY 1 cut(s) 285
BstZ17I GTATAC 2 cut(s) 558, 652
BstZI CGGCCG 1 cut(s) 853
Bsu15I ATCGAT 1 cut(s) 1043
Bsu36I CCTNAGG 1 cut(s) 1069
BsuRI GGCC 7 cut(s) 759, 774, 804, 810, 855, 1002, 1307
BsuTUI ATCGAT 1 cut(s) 1043
BtgI CCRYGG 2 cut(s) 414, 684
BtsCI GGATG 7 cut(s) 336, 359, 363, 539, 1012, 1144, 1288
BtsIMutI CAGTG 2 cut(s) 19, 491
BveI ACCTGC 2 cut(s) 167, 1387
Cac8I GCNNGC 1 cut(s) 1173
CciI TCATGA 1 cut(s) 181
CfoI GCGC 2 cut(s) 893, 1183
Cfr10I RCCGGY 2 cut(s) 896, 1415
Cfr13I GGNCC 8 cut(s) 224, 298, 802, 808, 809, 926, 990, 1074
ClaI ATCGAT 1 cut(s) 1043
CseI GACGC 2 cut(s) 365, 981
Csp6I GTAC 7 cut(s) 49, 212, 612, 850, 979, 1123, 1234
CspAI ACCGGT 1 cut(s) 896
CviAII CATG 5 cut(s) 182, 221, 228, 507, 949
CviQI GTAC 7 cut(s) 49, 212, 612, 850, 979, 1123, 1234
DdeI CTNAG 9 cut(s) 134, 234, 282, 483, 905, 982, 1069, 1176, 1211
DpnI GATC 1 cut(s) 287
DpnII GATC 1 cut(s) 285
DrdI GACNNNNNNGTC 1 cut(s) 718
DriI GACNNNNNGTC 1 cut(s) 486
DseDI GACNNNNNNGTC 1 cut(s) 718
EaeI YGGCCR 3 cut(s) 757, 853, 1305
EagI CGGCCG 1 cut(s) 853
Eam1104I CTCTTC 2 cut(s) 97, 752
Eam1105I GACNNNNNGTC 1 cut(s) 486
EarI CTCTTC 2 cut(s) 97, 752
EciI GGCGGA 1 cut(s) 939
EclXI CGGCCG 1 cut(s) 853
Eco147I AGGCCT 1 cut(s) 774
Eco24I GRGCYC 4 cut(s) 395, 428, 528, 812
Eco47I GGWCC 5 cut(s) 224, 298, 926, 990, 1074
Eco52I CGGCCG 1 cut(s) 853
Eco72I CACGTG 1 cut(s) 1200
Eco81I CCTNAGG 1 cut(s) 1069
Eco88I CYCGRG 2 cut(s) 306, 394
Eco91I GGTNACC 1 cut(s) 899
EcoNI CCTNNNNNAGG 1 cut(s) 293
EcoO109I RGGNCCY 2 cut(s) 809, 990
EcoO65I GGTNACC 1 cut(s) 899
EcoRII CCWGG 2 cut(s) 804, 1002
EcoT38I GRGCYC 4 cut(s) 395, 428, 528, 812
FaeI CATG 5 cut(s) 185, 224, 231, 510, 952
FaqI GGGAC 3 cut(s) 226, 1087, 1105
FatI CATG 5 cut(s) 181, 220, 227, 506, 948
FauI CCCGC 6 cut(s) 376, 439, 776, 968, 1176, 1208
FblI GTMKAC 2 cut(s) 557, 651
Fnu4HI GCNGC 7 cut(s) 28, 163, 382, 856, 922, 953, 1222
FokI GGATG 7 cut(s) 343, 346, 350, 526, 1019, 1151, 1295
FriOI GRGCYC 4 cut(s) 395, 428, 528, 812
Fsp4HI GCNGC 7 cut(s) 28, 163, 382, 856, 922, 953, 1222
GlaI GCGC 2 cut(s) 892, 1182
GluI GCNGC 7 cut(s) 28, 163, 382, 856, 922, 953, 1222
GsaI CCCAGC 3 cut(s) 247, 1341, 1442
GsuI CTGGAG 1 cut(s) 275
HaeII RGCGCY 1 cut(s) 1184
HaeIII GGCC 7 cut(s) 759, 774, 804, 810, 855, 1002, 1307
HapII CCGG 7 cut(s) 328, 378, 462, 792, 897, 1112, 1416
HgaI GACGC 2 cut(s) 365, 981
HhaI GCGC 2 cut(s) 893, 1183
Hin1I GRCGYC 2 cut(s) 1033, 1410
Hin1II CATG 5 cut(s) 185, 224, 231, 510, 952
Hin6I GCGC 2 cut(s) 891, 1181
HinP1I GCGC 2 cut(s) 891, 1181
HincII GTYRAC 3 cut(s) 491, 711, 723
HindII GTYRAC 3 cut(s) 491, 711, 723
HinfI GANTC 5 cut(s) 309, 481, 690, 1017, 1045
HpaII CCGG 7 cut(s) 328, 378, 462, 792, 897, 1112, 1416
HphI GGTGA 3 cut(s) 304, 347, 420
Hpy166II GTNNAC 6 cut(s) 214, 491, 558, 652, 711, 723
Hpy188I TCNGA 2 cut(s) 120, 285
Hpy188III TCNNGA 3 cut(s) 182, 396, 969
Hpy8I GTNNAC 6 cut(s) 214, 491, 558, 652, 711, 723
Hpy99I CGWCG 3 cut(s) 359, 1038, 1415
HpyAV CCTTC 3 cut(s) 43, 187, 514
HpyCH4III ACNGT 2 cut(s) 415, 1392
HpyCH4IV ACGT 7 cut(s) 342, 719, 1023, 1033, 1199, 1302, 1410
HpyCH4V TGCA 3 cut(s) 162, 627, 1396
HpyF10VI GCNNNNNNNGC 5 cut(s) 390, 633, 780, 1127, 1221
HpyF3I CTNAG 9 cut(s) 134, 234, 282, 483, 905, 982, 1069, 1176, 1211
HpySE526I ACGT 7 cut(s) 342, 719, 1023, 1033, 1199, 1302, 1410
Hsp92I GRCGYC 2 cut(s) 1033, 1410
Hsp92II CATG 5 cut(s) 185, 224, 231, 510, 952
HspAI GCGC 2 cut(s) 891, 1181
KpnI GGTACC 1 cut(s) 615
Kzo9I GATC 1 cut(s) 285
LmnI GCTCC 4 cut(s) 35, 465, 523, 1218
Lsp1109I GCAGC 4 cut(s) 14, 174, 393, 1233
LweI GCATC 3 cut(s) 161, 368, 1273
MaeII ACGT 7 cut(s) 342, 719, 1023, 1033, 1199, 1302, 1410
MaeIII GTNAC 5 cut(s) 310, 335, 409, 509, 899
MalI GATC 1 cut(s) 287
MbiI CCGCTC 2 cut(s) 955, 1215
MboI GATC 1 cut(s) 285
MboII GAAGA 4 cut(s) 84, 202, 739, 853
MflI RGATCY 1 cut(s) 285
MhlI GDGCHC 5 cut(s) 395, 428, 528, 812, 844
MlsI TGGCCA 2 cut(s) 759, 1307
MluCI AATT 1 cut(s) 574
MluNI TGGCCA 2 cut(s) 759, 1307
MlyI GAGTC 2 cut(s) 318, 475
MmeI TCCRAC 2 cut(s) 296, 994
Mox20I TGGCCA 2 cut(s) 759, 1307
MscI TGGCCA 2 cut(s) 759, 1307
MseI TTAA 3 cut(s) 81, 204, 1146
MslI CAYNNNNRTG 2 cut(s) 219, 1321
Msp20I TGGCCA 2 cut(s) 759, 1307
MspA1I CMGCKG 3 cut(s) 1029, 1260, 1442
MspI CCGG 7 cut(s) 328, 378, 462, 792, 897, 1112, 1416
MspR9I CCNGG 5 cut(s) 328, 463, 793, 806, 1004
MvaI CCWGG 2 cut(s) 806, 1004
MvnI CGCG 2 cut(s) 975, 1105
MwoI GCNNNNNNNGC 5 cut(s) 390, 633, 780, 1127, 1221
NciI CCSGG 3 cut(s) 328, 463, 793
NdeII GATC 1 cut(s) 285
NlaIII CATG 5 cut(s) 185, 224, 231, 510, 952
NlaIV GGNNCC 6 cut(s) 525, 613, 810, 928, 991, 1075
NmuCI GTSAC 3 cut(s) 310, 335, 409
OliI CACNNNNGTG 1 cut(s) 1321
PagI TCATGA 1 cut(s) 181
PceI AGGCCT 1 cut(s) 774
PcsI WCGNNNNNNNCGW 1 cut(s) 1061
PfeI GAWTC 3 cut(s) 690, 1017, 1045
PfoI TCCNGGA 1 cut(s) 326
PinAI ACCGGT 1 cut(s) 896
PkrI GCNGC 7 cut(s) 29, 164, 383, 857, 923, 954, 1223
PleI GAGTC 2 cut(s) 317, 475
PmaCI CACGTG 1 cut(s) 1200
PmlI CACGTG 1 cut(s) 1200
PpsI GAGTC 2 cut(s) 317, 475
Ppu21I YACGTR 2 cut(s) 1200, 1303
PpuMI RGGWCCY 1 cut(s) 990
Psp5II RGGWCCY 1 cut(s) 990
Psp6I CCWGG 2 cut(s) 804, 1002
PspCI CACGTG 1 cut(s) 1200
PspEI GGTNACC 1 cut(s) 899
PspFI CCCAGC 3 cut(s) 243, 1337, 1438
PspGI CCWGG 2 cut(s) 804, 1002
PspN4I GGNNCC 6 cut(s) 525, 613, 810, 928, 991, 1075
PspOMI GGGCCC 1 cut(s) 808
PspPI GGNCC 8 cut(s) 224, 298, 802, 808, 809, 926, 990, 1074
PspPPI RGGWCCY 1 cut(s) 990
PstI CTGCAG 1 cut(s) 164
PsuI RGATCY 1 cut(s) 285
PvuII CAGCTG 1 cut(s) 1442
RsaI GTAC 7 cut(s) 50, 213, 613, 851, 980, 1124, 1235
RsaNI GTAC 7 cut(s) 49, 212, 612, 850, 979, 1123, 1234
RseI CAYNNNNRTG 2 cut(s) 219, 1321
SaqAI TTAA 3 cut(s) 81, 204, 1146
SatI GCNGC 7 cut(s) 28, 163, 382, 856, 922, 953, 1222
Sau3AI GATC 1 cut(s) 285
Sau96I GGNCC 8 cut(s) 224, 298, 802, 808, 809, 926, 990, 1074
ScaI AGTACT 1 cut(s) 50
SchI GAGTC 2 cut(s) 318, 475
ScrFI CCNGG 5 cut(s) 328, 463, 793, 806, 1004
SduI GDGCHC 5 cut(s) 395, 428, 528, 812, 844
SfaNI GCATC 3 cut(s) 161, 368, 1273
SfcI CTRYAG 1 cut(s) 160
SgrAI CRCCGGYG 1 cut(s) 1415
SinI GGWCC 5 cut(s) 224, 298, 926, 990, 1074
SmiMI CAYNNNNRTG 2 cut(s) 219, 1321
Sse9I AATT 1 cut(s) 574
SseBI AGGCCT 1 cut(s) 774
SspI AATATT 1 cut(s) 85
StuI AGGCCT 1 cut(s) 774
StyD4I CCNGG 5 cut(s) 326, 461, 791, 804, 1002
TaaI ACNGT 2 cut(s) 415, 1392
TaiI ACGT 7 cut(s) 345, 722, 1026, 1036, 1202, 1305, 1413
TaqI TCGA 5 cut(s) 155, 735, 831, 1043, 1187
TasI AATT 1 cut(s) 574
TatI WGTACW 1 cut(s) 48
TauI GCSGC 3 cut(s) 858, 924, 955
TfiI GAWTC 3 cut(s) 690, 1017, 1045
Tru1I TTAA 3 cut(s) 81, 204, 1146
Tru9I TTAA 3 cut(s) 81, 204, 1146
TscAI CASTG 2 cut(s) 19, 491
TseFI GTSAC 3 cut(s) 310, 335, 409
TseI GCWGC 4 cut(s) 27, 162, 381, 1221
Tsp45I GTSAC 3 cut(s) 310, 335, 409
TspDTI ATGAA 3 cut(s) 8, 937, 1172
TspGWI ACGGA 2 cut(s) 673, 740
TspRI CASTG 2 cut(s) 19, 491
VpaK11BI GGWCC 5 cut(s) 224, 298, 926, 990, 1074
XagI CCTNNNNNAGG 1 cut(s) 293
XmiI GTMKAC 2 cut(s) 557, 651
ZraI GACGTC 2 cut(s) 1034, 1411
ZrmI AGTACT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.