MD15G1044100.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000845
Physical Location & Seq
Forward (+)
3044051 .. 3046906
2856 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1044100.v1.1.491

Sequence Viewer

Length: 1467 bp
ATGGCAACTCCCATTTCTATCTCCCCCTCCTTCCTCAGGGTTTTCTTTGGTTGCTTTGTGTTTTTTATCTGCGTAATTCTCCGCTCGCCGGAGAAGGGCTTTGCTTCGGCAGAAGGAGATACAAAAACTGACCTTCTTCCACATACTCACACAGTTGAAGTAAACTCTCTGCAACCAGCAACCACCTGCATCCTCTCCACCCAAGGTCCTTACAATAATAAGGAATCAGTATTGGAAGTGGTACACAAGCATGGGCTATGCAATCAACACAAATCACAAAGTAACCCTACTGATCAGGATCGCACTCAAATCCTAAAGCAAGACGAAGCCCGAGTCAGCTCAATTCACTACCGTCTTAACTCACTTACACTACCTGACGCCGCCACCAATACCATCCCCGTACAGTATGGTCTGGAACTTGGATCATCCAACTACATTGTAACAGTGGGCCTCGGCACACCGGCCAAAAACCTCTCCCTCCTTATCGACACAGGCAGCGACCTAACTTGGACACAATGCCGACCGTGTGTCCGATCTTGTTACAAACAGAAAGAGCCAATGTTCGACCCCTCTCACTCCACCTCATACTCCAACATCTCCTGTGAGTCCGCTGCGTGTTCTCAGCTCACAGTGGCCGGCATTGGCCGCGACTGCTCCGCCGCCACTTGCACTTACAGCGCCAAGTATTTAGACAACTCCTCCTCACTCGGCTTCTTCGGCAATGACAAGCTAACATTAACACCGACGGATGCTTACGATGATTTCGATTTTGGCTGCGCGCAAAACAGCAAAGGCCAGTTCGGCGCGGCCGCCGGTTTGCTCGGACTGGGCCGGACCAGCATCTCCTTCGTCGAACAAACCTCCCAGAAATACAAACGAGTATTCTCCTACTGTCTACCTTCCACTTCAAGCTCCACCGGCTACCTCAAATTCGGCCCCGACGGAGGCTCCGGTAACGCAGTCAAGTTCACGCCGCTCAAAACTTTATCCCAAAGCGCTACATTTTACGCCCTTGACCTAGTTGGCATCACCATCGGTAGCCGTCAACTGAAGATTTCAGCCTCTGTCTTTTCAAAGTCGGGGACAGTCATCGACTCGGGGACAGTGATCACGCGCCTCCCGGCCACCCCATATGCCGCATTACGCGTGGCTTTTAGAAAAGCGATGGAGAAGTACCCTTTTGCGAAGTCGAAGTCTGCCCTACTAGACACTTGCTATGACTTTAGTGGACTTAAAACGGTGTCGTACCCGAAGATAGGGTTTGTATTTGGAGGTGGGGTTACGGTGGACTTGGACGCGACAGGGATACTGTTCCAGACGAGTGGTTCGCAGTTTTGCTTGGCGTTCGCCGGAAACAAGGACGATAATAGCGTCGGGATTATCGGCAACGTTCAGCAGAAGAGGCTGCAAGTGGTGTACGACGTTGCTGGGGGAAGGATCGGATTTGGCTCTGCAGGTTGCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

489

Amino Acids

52.2

Weight (kDa)

8.48

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 144 - 198 5e-08 Eukaryotic aspartyl protease
TAXi_N PF14543 145 - 312 1.8e-52 Xylanase inhibitor N-terminal
Asp PF00026 224 - 484 5e-15 Eukaryotic aspartyl protease
TAXi_C PF14541 336 - 483 1e-28 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 194
Acc36I ACCTGC 2 cut(s) 194, 1445
AccBSI CCGCTC 2 cut(s) 84, 976
AccI GTMKAC 1 cut(s) 895
AccII CGCG 6 cut(s) 648, 779, 806, 1114, 1146, 1298
AclI AACGTT 1 cut(s) 1389
AclWI GGATC 3 cut(s) 306, 430, 1445
AcoI YGGCCR 5 cut(s) 462, 633, 643, 807, 1122
AcsI RAATTY 1 cut(s) 929
AcuI CTGAAG 1 cut(s) 1070
AcyI GRCGYC 1 cut(s) 378
AfaI GTAC 5 cut(s) 243, 402, 1175, 1247, 1418
AfeI AGCGCT 1 cut(s) 997
AfiI CCNNNNNNNGG 5 cut(s) 36, 88, 95, 944, 1256
AflIII ACRYGT 1 cut(s) 1144
AgsI TTSAA 3 cut(s) 158, 909, 1074
AhdI GACNNNNNGTC 1 cut(s) 527
AluBI AGCT 4 cut(s) 339, 625, 730, 912
AluI AGCT 4 cut(s) 339, 625, 730, 912
AlwI GGATC 3 cut(s) 306, 430, 1445
AlwNI CAGNNNCTG 1 cut(s) 1064
Ama87I CYCGRG 2 cut(s) 330, 1096
Aor51HI AGCGCT 1 cut(s) 997
AoxI GGCC 9 cut(s) 448, 462, 633, 643, 793, 807, 829, 934, 1122
ApeKI GCWGC 4 cut(s) 495, 611, 774, 1405
ApoI RAATTY 1 cut(s) 929
ArsI GACNNNNNNTTYG 6 cut(s) 318, 350, 1178, 1210, 1309, 1341
AspLEI GCGC 6 cut(s) 680, 779, 781, 806, 998, 1116
AspS9I GGNCC 5 cut(s) 206, 448, 829, 834, 935
AsuC2I CCSGG 1 cut(s) 1121
AsuHPI GGTGA 1 cut(s) 1021
AvaI CYCGRG 2 cut(s) 330, 1096
AvaII GGWCC 2 cut(s) 206, 834
AxyI CCTNAGG 1 cut(s) 35
BbvI GCAGC 4 cut(s) 507, 598, 761, 1392
BccI CCATC 3 cut(s) 401, 1040, 1159
BceAI ACGGC 1 cut(s) 1026
BcgI CGANNNNNNTGC 4 cut(s) 829, 863, 1015, 1049
BciVI GTATCC 1 cut(s) 1299
BclI TGATCA 2 cut(s) 292, 1107
BcnI CCSGG 1 cut(s) 1121
BfaI CTAG 2 cut(s) 1019, 1205
BfmI CTRYAG 1 cut(s) 1452
BfoI RGCGCY 2 cut(s) 681, 999
BfuAI ACCTGC 2 cut(s) 194, 1445
BfuI GTATCC 1 cut(s) 1299
BglI GCCNNNNNGGC 1 cut(s) 801
Bme1390I CCNGG 1 cut(s) 1121
Bme18I GGWCC 2 cut(s) 206, 834
BmeRI GACNNNNNGTC 1 cut(s) 527
BmeT110I CYCGRG 2 cut(s) 330, 1096
BmgT120I GGNCC 5 cut(s) 206, 448, 829, 834, 935
BmiI GGNNCC 2 cut(s) 937, 949
BmrFI CCNGG 1 cut(s) 1121
BmrI ACTGGG 1 cut(s) 836
BmsI GCATC 4 cut(s) 198, 739, 849, 1035
BmuI ACTGGG 1 cut(s) 836
BpuMI CCSGG 1 cut(s) 1121
BsaBI GATNNNNATC 1 cut(s) 297
BsaHI GRCGYC 1 cut(s) 378
BsaJI CCNNGG 2 cut(s) 202, 451
BsaWI WCCGGW 1 cut(s) 950
BsaXI ACNNNNNCTCC 6 cut(s) 683, 713, 932, 962, 1263, 1293
Bsc4I CCNNNNNNNGG 5 cut(s) 36, 88, 95, 944, 1256
Bse118I RCCGGY 4 cut(s) 460, 635, 812, 917
Bse1I ACTGG 2 cut(s) 796, 831
Bse21I CCTNAGG 1 cut(s) 35
Bse3DI GCAATG 1 cut(s) 727
Bse8I GATNNNNATC 1 cut(s) 297
BseDI CCNNGG 2 cut(s) 202, 451
BseGI GGATG 4 cut(s) 189, 393, 425, 754
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 5 cut(s) 36, 88, 95, 944, 1256
BseMI GCAATG 1 cut(s) 727
BseMII CTCAG 2 cut(s) 49, 635
BseNI ACTGG 2 cut(s) 796, 831
BsePI GCGCGC 1 cut(s) 777
BseRI GAGGAG 2 cut(s) 688, 691
BseX3I CGGCCG 1 cut(s) 807
BseXI GCAGC 4 cut(s) 507, 598, 761, 1392
BseYI CCCAGC 1 cut(s) 1427
Bsh1236I CGCG 6 cut(s) 648, 779, 806, 1114, 1146, 1298
Bsh1285I CGRYCG 2 cut(s) 524, 810
BshFI GGCC 9 cut(s) 450, 464, 635, 645, 795, 809, 831, 936, 1124
BsiEI CGRYCG 2 cut(s) 524, 810
BsiHKCI CYCGRG 2 cut(s) 330, 1096
BsiSI CCGG 9 cut(s) 89, 461, 636, 813, 832, 918, 951, 1121, 1350
BslFI GGGAC 2 cut(s) 1096, 1114
BslI CCNNNNNNNGG 5 cut(s) 36, 88, 95, 944, 1256
BsmFI GGGAC 2 cut(s) 1096, 1114
BsnI GGCC 9 cut(s) 450, 464, 635, 645, 795, 809, 831, 936, 1124
BsoBI CYCGRG 2 cut(s) 330, 1096
Bsp143I GATC 6 cut(s) 292, 298, 422, 533, 1107, 1437
BspANI GGCC 9 cut(s) 450, 464, 635, 645, 795, 809, 831, 936, 1124
BspCNI CTCAG 2 cut(s) 48, 634
BspFNI CGCG 6 cut(s) 648, 779, 806, 1114, 1146, 1298
BspLI GGNNCC 2 cut(s) 937, 949
BspMAI CTGCAG 1 cut(s) 1456
BspMI ACCTGC 2 cut(s) 194, 1445
BspPI GGATC 3 cut(s) 306, 430, 1445
BsrBI CCGCTC 2 cut(s) 84, 976
BsrDI GCAATG 1 cut(s) 727
BsrFI RCCGGY 4 cut(s) 460, 635, 812, 917
BsrI ACTGG 2 cut(s) 796, 831
BssAI RCCGGY 4 cut(s) 460, 635, 812, 917
BssECI CCNNGG 2 cut(s) 202, 451
BssHII GCGCGC 1 cut(s) 777
BssMI GATC 6 cut(s) 292, 298, 422, 533, 1107, 1437
BssNI GRCGYC 1 cut(s) 378
BssT1I CCWWGG 1 cut(s) 202
Bst6I CTCTTC 1 cut(s) 1394
BstACI GRCGYC 1 cut(s) 378
BstC8I GCNNGC 3 cut(s) 86, 637, 779
BstDEI CTNAG 2 cut(s) 35, 621
BstENI CCTNNNNNAGG 1 cut(s) 34
BstF5I GGATG 4 cut(s) 189, 393, 425, 754
BstFNI CGCG 6 cut(s) 648, 779, 806, 1114, 1146, 1298
BstH2I RGCGCY 2 cut(s) 681, 999
BstHHI GCGC 6 cut(s) 680, 779, 781, 806, 998, 1116
BstKTI GATC 6 cut(s) 295, 301, 425, 536, 1110, 1440
BstMBI GATC 6 cut(s) 292, 298, 422, 533, 1107, 1437
BstMCI CGRYCG 2 cut(s) 524, 810
BstMWI GCNNNNNNNGC 8 cut(s) 645, 651, 675, 717, 801, 837, 918, 1402
BstSCI CCNGG 1 cut(s) 1119
BstSFI CTRYAG 1 cut(s) 1452
BstUI CGCG 6 cut(s) 648, 779, 806, 1114, 1146, 1298
BstV1I GCAGC 4 cut(s) 507, 598, 761, 1392
BstXI CCANNNNNNTGG 1 cut(s) 1322
BstZI CGGCCG 1 cut(s) 807
Bsu36I CCTNAGG 1 cut(s) 35
BsuI GTATCC 1 cut(s) 1299
BsuRI GGCC 9 cut(s) 450, 464, 635, 645, 795, 809, 831, 936, 1124
BtgZI GCGATG 1 cut(s) 1178
BtsCI GGATG 4 cut(s) 189, 393, 425, 754
BtsIMutI CAGTG 3 cut(s) 450, 636, 1110
BveI ACCTGC 2 cut(s) 194, 1445
Cac8I GCNNGC 3 cut(s) 86, 637, 779
CaiI CAGNNNCTG 1 cut(s) 1064
CciNI GCGGCCGC 1 cut(s) 807
CfoI GCGC 6 cut(s) 680, 779, 781, 806, 998, 1116
Cfr10I RCCGGY 4 cut(s) 460, 635, 812, 917
Cfr13I GGNCC 5 cut(s) 206, 448, 829, 834, 935
CseI GACGC 3 cut(s) 386, 1304, 1360
Csp6I GTAC 5 cut(s) 242, 401, 1174, 1246, 1417
CviAII CATG 1 cut(s) 251
CviQI GTAC 5 cut(s) 242, 401, 1174, 1246, 1417
DdeI CTNAG 2 cut(s) 35, 621
DpnI GATC 6 cut(s) 294, 300, 424, 535, 1109, 1439
DpnII GATC 6 cut(s) 292, 298, 422, 533, 1107, 1437
DriI GACNNNNNGTC 1 cut(s) 527
EaeI YGGCCR 5 cut(s) 462, 633, 643, 807, 1122
EagI CGGCCG 1 cut(s) 807
Eam1104I CTCTTC 1 cut(s) 1394
Eam1105I GACNNNNNGTC 1 cut(s) 527
EarI CTCTTC 1 cut(s) 1394
EciI GGCGGA 1 cut(s) 646
EclXI CGGCCG 1 cut(s) 807
Eco130I CCWWGG 1 cut(s) 202
Eco47I GGWCC 2 cut(s) 206, 834
Eco47III AGCGCT 1 cut(s) 997
Eco52I CGGCCG 1 cut(s) 807
Eco57I CTGAAG 1 cut(s) 1070
Eco81I CCTNAGG 1 cut(s) 35
Eco88I CYCGRG 2 cut(s) 330, 1096
EcoNI CCTNNNNNAGG 1 cut(s) 34
EcoO109I RGGNCCY 1 cut(s) 206
EcoT14I CCWWGG 1 cut(s) 202
ErhI CCWWGG 1 cut(s) 202
FaeI CATG 1 cut(s) 254
FaiI YATR 8 cut(s) 144, 252, 259, 408, 586, 1132, 1134, 1218
FaqI GGGAC 2 cut(s) 1096, 1114
FatI CATG 1 cut(s) 250
FauNDI CATATG 1 cut(s) 1132
FbaI TGATCA 2 cut(s) 292, 1107
FblI GTMKAC 1 cut(s) 895
FokI GGATG 4 cut(s) 176, 380, 412, 761
FspBI CTAG 2 cut(s) 1019, 1205
GlaI GCGC 6 cut(s) 679, 778, 780, 805, 997, 1115
GsaI CCCAGC 1 cut(s) 1431
HaeII RGCGCY 2 cut(s) 681, 999
HaeIII GGCC 9 cut(s) 450, 464, 635, 645, 795, 809, 831, 936, 1124
HapII CCGG 9 cut(s) 89, 461, 636, 813, 832, 918, 951, 1121, 1350
HgaI GACGC 3 cut(s) 386, 1304, 1360
HhaI GCGC 6 cut(s) 680, 779, 781, 806, 998, 1116
Hin1I GRCGYC 1 cut(s) 378
Hin1II CATG 1 cut(s) 254
Hin6I GCGC 6 cut(s) 678, 777, 779, 804, 996, 1114
HinP1I GCGC 6 cut(s) 678, 777, 779, 804, 996, 1114
HincII GTYRAC 1 cut(s) 1046
HindII GTYRAC 1 cut(s) 1046
HinfI GANTC 4 cut(s) 224, 333, 605, 1094
HpaII CCGG 9 cut(s) 89, 461, 636, 813, 832, 918, 951, 1121, 1350
HphI GGTGA 1 cut(s) 1021
Hpy166II GTNNAC 8 cut(s) 163, 244, 896, 969, 1046, 1229, 1288, 1417
Hpy188I TCNGA 3 cut(s) 533, 824, 1442
Hpy188III TCNNGA 4 cut(s) 296, 413, 1315, 1375
Hpy8I GTNNAC 8 cut(s) 163, 244, 896, 969, 1046, 1229, 1288, 1417
Hpy99I CGWCG 5 cut(s) 748, 854, 944, 1376, 1424
HpyAV CCTTC 7 cut(s) 40, 88, 107, 143, 856, 909, 1428
HpyCH4IV ACGT 2 cut(s) 1389, 1422
HpyCH4V TGCA 6 cut(s) 172, 189, 261, 669, 1408, 1454
HpyF10VI GCNNNNNNNGC 8 cut(s) 645, 651, 675, 717, 801, 837, 918, 1402
HpyF3I CTNAG 2 cut(s) 35, 621
HpySE526I ACGT 2 cut(s) 1389, 1422
Hsp92I GRCGYC 1 cut(s) 378
Hsp92II CATG 1 cut(s) 254
HspAI GCGC 6 cut(s) 678, 777, 779, 804, 996, 1114
KroI GCCGGC 1 cut(s) 635
KroNI GCCGGC 1 cut(s) 637
Ksp22I TGATCA 2 cut(s) 292, 1107
Kzo9I GATC 6 cut(s) 292, 298, 422, 533, 1107, 1437
LmnI GCTCC 3 cut(s) 659, 917, 953
Lsp1109I GCAGC 4 cut(s) 507, 598, 761, 1392
LweI GCATC 4 cut(s) 198, 739, 849, 1035
MaeI CTAG 2 cut(s) 1019, 1205
MaeII ACGT 2 cut(s) 1389, 1422
MaeIII GTNAC 5 cut(s) 281, 439, 539, 953, 1279
MalI GATC 6 cut(s) 294, 300, 424, 535, 1109, 1439
MbiI CCGCTC 2 cut(s) 84, 976
MboI GATC 6 cut(s) 292, 298, 422, 533, 1107, 1437
MboII GAAGA 5 cut(s) 128, 706, 1063, 1264, 1411
MluCI AATT 3 cut(s) 75, 342, 929
MluI ACGCGT 1 cut(s) 1144
MlyI GAGTC 3 cut(s) 342, 614, 1088
MmeI TCCRAC 2 cut(s) 453, 615
MroNI GCCGGC 1 cut(s) 635
MseI TTAA 3 cut(s) 357, 737, 1233
MslI CAYNNNNRTG 1 cut(s) 249
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 9 cut(s) 89, 461, 636, 813, 832, 918, 951, 1121, 1350
MspR9I CCNGG 1 cut(s) 1121
MvnI CGCG 6 cut(s) 648, 779, 806, 1114, 1146, 1298
MwoI GCNNNNNNNGC 8 cut(s) 645, 651, 675, 717, 801, 837, 918, 1402
NaeI GCCGGC 1 cut(s) 637
NciI CCSGG 1 cut(s) 1121
NdeI CATATG 1 cut(s) 1132
NdeII GATC 6 cut(s) 292, 298, 422, 533, 1107, 1437
NgoMIV GCCGGC 1 cut(s) 635
NlaIII CATG 1 cut(s) 254
NlaIV GGNNCC 2 cut(s) 937, 949
NmeAIII GCCGAG 2 cut(s) 432, 687
NotI GCGGCCGC 1 cut(s) 807
PaqCI CACCTGC 1 cut(s) 194
PauI GCGCGC 1 cut(s) 777
PcsI WCGNNNNNNNCGW 2 cut(s) 762, 1368
PdiI GCCGGC 1 cut(s) 637
PfeI GAWTC 1 cut(s) 224
PleI GAGTC 3 cut(s) 341, 613, 1088
PpsI GAGTC 3 cut(s) 341, 613, 1088
PpuMI RGGWCCY 1 cut(s) 206
Psp1406I AACGTT 1 cut(s) 1389
Psp5II RGGWCCY 1 cut(s) 206
PspFI CCCAGC 1 cut(s) 1427
PspN4I GGNNCC 2 cut(s) 937, 949
PspPI GGNCC 5 cut(s) 206, 448, 829, 834, 935
PspPPI RGGWCCY 1 cut(s) 206
PstI CTGCAG 1 cut(s) 1456
PstNI CAGNNNCTG 1 cut(s) 1064
PteI GCGCGC 1 cut(s) 777
RsaI GTAC 5 cut(s) 243, 402, 1175, 1247, 1418
RsaNI GTAC 5 cut(s) 242, 401, 1174, 1246, 1417
RseI CAYNNNNRTG 1 cut(s) 249
SaqAI TTAA 3 cut(s) 357, 737, 1233
Sau3AI GATC 6 cut(s) 292, 298, 422, 533, 1107, 1437
Sau96I GGNCC 5 cut(s) 206, 448, 829, 834, 935
SchI GAGTC 3 cut(s) 342, 614, 1088
ScrFI CCNGG 1 cut(s) 1121
SfaNI GCATC 4 cut(s) 198, 739, 849, 1035
SfcI CTRYAG 1 cut(s) 1452
SinI GGWCC 2 cut(s) 206, 834
SmiMI CAYNNNNRTG 1 cut(s) 249
Sse9I AATT 3 cut(s) 75, 342, 929
SspMI CTAG 2 cut(s) 1019, 1205
StyD4I CCNGG 1 cut(s) 1119
StyI CCWWGG 1 cut(s) 202
TaiI ACGT 2 cut(s) 1392, 1425
TaqI TCGA 6 cut(s) 486, 564, 765, 852, 1092, 1190
TasI AATT 3 cut(s) 75, 342, 929
TauI GCSGC 7 cut(s) 383, 648, 662, 809, 812, 976, 1139
TfiI GAWTC 1 cut(s) 224
Tru1I TTAA 3 cut(s) 357, 737, 1233
Tru9I TTAA 3 cut(s) 357, 737, 1233
TscAI CASTG 3 cut(s) 450, 636, 1110
TseI GCWGC 4 cut(s) 495, 611, 774, 1405
TspGWI ACGGA 2 cut(s) 761, 957
TspRI CASTG 3 cut(s) 450, 636, 1110
VpaK11BI GGWCC 2 cut(s) 206, 834
XagI CCTNNNNNAGG 1 cut(s) 34
XapI RAATTY 1 cut(s) 929
XmiI GTMKAC 1 cut(s) 895
XspI CTAG 2 cut(s) 1019, 1205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.