pycom08g04300

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000845
Physical Location & Seq
Reverse (-)
3310778 .. 3312042
1265 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g04300.1

Sequence Viewer

Length: 1224 bp
ATGGCCCATGCAACCAATTCCACCAACTCAAATCAAACAGTAACCCTGCCGATCAAGATGAATATCACACACAAATCCTCAAGCAAGACGAAGCCCGAGTCAACTCAATCCACTCCCATTTCAACGACCCAATCCATAATTACAGAATCCCCGTCCCACTTCGTACTTGGTTCAGGAAACTACATCGTCAGTGTTGGCTTGGGCTCACCCCAGAAGCAACTCTCGCTTGTATTCGACACCGGCAGCAGTCTCACTTGGACGCAGTGCCGACCATGTTCTGTATCTTGTTACCAACAGAAAGATCCCATCTTCGACCCTTCTCTCTCTACCTCTTACTCCAACATCTCCTGCAACTCCGCCGCGTGTTCCCAACTTGCAGCCTCCGGTGTCGCTAGCAATTGTTCCGCCTCCACCTGTGTTTACGGCCAACAGTATGGAGACCGATCGATCACAGTCGGCTTCTTTGCCAGCGAGAAGTTAACTTTGACTTCAACAGATGTTTTTAATGGTTTCGTCTTCGGCTGCGGCCAGAATAACCAAGGCTTATTTAAGGGCATTGCCGGTTTGCTCGGTCTCGGTCGAAGCAAGATCTCCCTCGTTGAACAAAGAGCTCAGAAGTACAACAGATTCTTCTCCTACTGCCTCCCCTCCACTTCAAGCTCCACCGGCTACCTCAGCTTAGGCAACTCCGACAATGGACGTTCAGGATATAACGCCGTCAAGTTCACACCGCTCACCACATTATCTCGAGGCGAGTCATATTACGGCCTCGGACTGGTCGGTATCAGCATCGGTGGGAGTCAACTGCCAATTTCAGCTTCGGTATTTTCGACTTCGGGAACAATTATCGACTCGGGGACTGTGATCACGCGCCTCCCAGCCACTGCGTACACAGCGCTGCGGAACGCATTTCGAGAAGCGATGAAGAGGTACCCAACTGCTAAGTCGATTTCTTCCCTGCTGGACACGTGTTACGATTTCAGTGGAATCAAAACGATAACCTTTCCAAAGATAGGGTTTGTGTTTGGCAATGGGGTTACGGTAGACGTGGACGCAACAGGAATATTAGTCCAGCAGAGTGCTTCGCAGTTTTGCTTGGCGTTTGCCGGAAACAAAGATGATAGGAGTGTCGGGATTGTTGGGAACGTTCAACAGAAGAGGTTGCAGGTAGTGTATGACGTTGCTGGTGGAAAGGTTGGATTTGCCCCTGATGGTTGCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

408

Amino Acids

42.96

Weight (kDa)

9.0

Isoelectric Point (pI)

34.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 60 - 403 4.8e-24 Eukaryotic aspartyl protease
TAXi_N PF14543 61 - 229 1.2e-52 Xylanase inhibitor N-terminal
TAXi_C PF14541 255 - 402 3.2e-30 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1156
Acc65I GGTACC 1 cut(s) 930
AccB1I GGYRCC 1 cut(s) 930
AccBSI CCGCTC 1 cut(s) 733
AccI GTMKAC 1 cut(s) 1044
AccII CGCG 2 cut(s) 362, 871
AciI CCGC 6 cut(s) 357, 360, 405, 525, 731, 901
AclI AACGTT 1 cut(s) 1146
AclWI GGATC 1 cut(s) 296
AcoI YGGCCR 2 cut(s) 424, 526
AcvI CACGTG 1 cut(s) 969
AfaI GTAC 4 cut(s) 165, 620, 890, 932
AfeI AGCGCT 1 cut(s) 897
AfiI CCNNNNNNNGG 2 cut(s) 775, 1013
AflIII ACRYGT 2 cut(s) 966, 968
AgsI TTSAA 5 cut(s) 123, 492, 602, 657, 1151
AjiI CACGTC 1 cut(s) 1048
AjuI GAANNNNNNNTTGG 2 cut(s) 802, 834
AluBI AGCT 4 cut(s) 611, 660, 678, 818
AluI AGCT 4 cut(s) 611, 660, 678, 818
Alw21I GWGCWC 1 cut(s) 613
Alw26I GTCTC 3 cut(s) 254, 432, 578
AlwI GGATC 1 cut(s) 296
AlwNI CAGNNNCTG 1 cut(s) 884
Ama87I CYCGRG 3 cut(s) 95, 747, 853
Aor51HI AGCGCT 1 cut(s) 897
AoxI GGCC 4 cut(s) 3, 424, 526, 766
ApeKI GCWGC 4 cut(s) 243, 377, 522, 898
ArsI GACNNNNNNTTYG 2 cut(s) 83, 115
Asp718I GGTACC 1 cut(s) 930
AspLEI GCGC 2 cut(s) 873, 898
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 2 cut(s) 198, 727
AsuNHI GCTAGC 1 cut(s) 392
AvaI CYCGRG 3 cut(s) 95, 747, 853
BanI GGYRCC 1 cut(s) 930
BanII GRGCYC 2 cut(s) 206, 613
BbrPI CACGTG 1 cut(s) 969
BbsI GAAGAC 1 cut(s) 508
Bbv12I GWGCWC 1 cut(s) 613
BbvCI CCTCAGC 1 cut(s) 674
BbvI GCAGC 4 cut(s) 255, 389, 509, 885
BccI CCATC 2 cut(s) 314, 1205
BceAI ACGGC 3 cut(s) 439, 701, 781
BclI TGATCA 1 cut(s) 864
BcoDI GTCTC 3 cut(s) 254, 432, 578
BfaI CTAG 1 cut(s) 393
BfoI RGCGCY 1 cut(s) 899
BfuAI ACCTGC 1 cut(s) 1156
BglII AGATCT 1 cut(s) 588
BisI GCNGC 6 cut(s) 244, 360, 378, 523, 526, 899
BlsI GCNGC 6 cut(s) 245, 361, 379, 524, 527, 900
BmeT110I CYCGRG 3 cut(s) 95, 747, 853
BmgBI CACGTC 1 cut(s) 1048
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 932
BmsI GCATC 1 cut(s) 798
BmtI GCTAGC 1 cut(s) 396
BpiI GAAGAC 1 cut(s) 508
Bpu10I CCTNAGC 2 cut(s) 674, 679
BpuEI CTTGAG 1 cut(s) 64
Bsa29I ATCGAT 1 cut(s) 446
BsaAI YACGTR 1 cut(s) 969
BsaBI GATNNNNATC 1 cut(s) 62
BsaI GGTCTC 2 cut(s) 432, 578
BsaJI CCNNGG 2 cut(s) 538, 769
BsaWI WCCGGW 1 cut(s) 383
Bsc4I CCNNNNNNNGG 2 cut(s) 775, 1013
Bse118I RCCGGY 3 cut(s) 239, 560, 665
Bse1I ACTGG 1 cut(s) 780
Bse3DI GCAATG 2 cut(s) 555, 1036
Bse8I GATNNNNATC 1 cut(s) 62
BseCI ATCGAT 1 cut(s) 446
BseDI CCNNGG 2 cut(s) 538, 769
BseJI GATNNNNATC 1 cut(s) 62
BseLI CCNNNNNNNGG 2 cut(s) 775, 1013
BseMI GCAATG 2 cut(s) 555, 1036
BseMII CTCAG 2 cut(s) 626, 688
BseNI ACTGG 1 cut(s) 780
BseXI GCAGC 4 cut(s) 255, 389, 509, 885
BseYI CCCAGC 1 cut(s) 877
Bsh1236I CGCG 2 cut(s) 362, 871
Bsh1285I CGRYCG 2 cut(s) 446, 580
BshFI GGCC 4 cut(s) 5, 426, 528, 768
BshNI GGYRCC 1 cut(s) 930
BshVI ATCGAT 1 cut(s) 446
BsiEI CGRYCG 2 cut(s) 446, 580
BsiHKAI GWGCWC 1 cut(s) 613
BsiHKCI CYCGRG 3 cut(s) 95, 747, 853
BsiSI CCGG 5 cut(s) 240, 384, 561, 666, 1107
BslFI GGGAC 2 cut(s) 139, 871
BslI CCNNNNNNNGG 2 cut(s) 775, 1013
BsmAI GTCTC 3 cut(s) 254, 432, 578
BsmFI GGGAC 2 cut(s) 139, 871
BsnI GGCC 4 cut(s) 5, 426, 528, 768
Bso31I GGTCTC 2 cut(s) 432, 578
BsoBI CYCGRG 3 cut(s) 95, 747, 853
Bsp1286I GDGCHC 2 cut(s) 206, 613
Bsp143I GATC 6 cut(s) 51, 301, 443, 447, 588, 864
BspACI CCGC 6 cut(s) 357, 360, 405, 525, 731, 901
BspANI GGCC 4 cut(s) 5, 426, 528, 768
BspCNI CTCAG 2 cut(s) 625, 687
BspDI ATCGAT 1 cut(s) 446
BspFNI CGCG 2 cut(s) 362, 871
BspLI GGNNCC 1 cut(s) 932
BspMI ACCTGC 1 cut(s) 1156
BspOI GCTAGC 1 cut(s) 396
BspPI GGATC 1 cut(s) 296
BspT107I GGYRCC 1 cut(s) 930
BspTNI GGTCTC 2 cut(s) 432, 578
BsrBI CCGCTC 1 cut(s) 733
BsrDI GCAATG 2 cut(s) 555, 1036
BsrFI RCCGGY 3 cut(s) 239, 560, 665
BsrI ACTGG 1 cut(s) 780
BssAI RCCGGY 3 cut(s) 239, 560, 665
BssECI CCNNGG 2 cut(s) 538, 769
BssMI GATC 6 cut(s) 51, 301, 443, 447, 588, 864
BssT1I CCWWGG 1 cut(s) 538
Bst4CI ACNGT 5 cut(s) 40, 432, 454, 862, 1042
Bst6I CTCTTC 2 cut(s) 920, 1151
BstBAI YACGTR 1 cut(s) 969
BstC8I GCNNGC 2 cut(s) 394, 469
BstDEI CTNAG 4 cut(s) 612, 674, 679, 942
BstFNI CGCG 2 cut(s) 362, 871
BstH2I RGCGCY 1 cut(s) 899
BstHHI GCGC 2 cut(s) 873, 898
BstKTI GATC 6 cut(s) 54, 304, 446, 450, 591, 867
BstMAI GTCTC 3 cut(s) 254, 432, 578
BstMBI GATC 6 cut(s) 51, 301, 443, 447, 588, 864
BstMCI CGRYCG 2 cut(s) 446, 580
BstMWI GCNNNNNNNGC 4 cut(s) 223, 666, 675, 893
BstUI CGCG 2 cut(s) 362, 871
BstV1I GCAGC 4 cut(s) 255, 389, 509, 885
BstV2I GAAGAC 1 cut(s) 508
BstX2I RGATCY 2 cut(s) 301, 588
BstXI CCANNNNNNTGG 1 cut(s) 434
BstYI RGATCY 2 cut(s) 301, 588
Bsu15I ATCGAT 1 cut(s) 446
BsuRI GGCC 4 cut(s) 5, 426, 528, 768
BsuTUI ATCGAT 1 cut(s) 446
BtgZI GCGATG 1 cut(s) 935
BtrI CACGTC 1 cut(s) 1048
BtsI GCAGTG 2 cut(s) 269, 882
BtsIMutI CAGTG 4 cut(s) 196, 269, 882, 988
BveI ACCTGC 1 cut(s) 1156
Cac8I GCNNGC 2 cut(s) 394, 469
CaiI CAGNNNCTG 1 cut(s) 884
CfoI GCGC 2 cut(s) 873, 898
Cfr10I RCCGGY 3 cut(s) 239, 560, 665
Cfr13I GGNCC 1 cut(s) 4
ClaI ATCGAT 1 cut(s) 446
CseI GACGC 2 cut(s) 268, 1061
Csp6I GTAC 4 cut(s) 164, 619, 889, 931
CviAII CATG 2 cut(s) 8, 273
CviQI GTAC 4 cut(s) 164, 619, 889, 931
DdeI CTNAG 4 cut(s) 612, 674, 679, 942
DpnI GATC 6 cut(s) 53, 303, 445, 449, 590, 866
DpnII GATC 6 cut(s) 51, 301, 443, 447, 588, 864
EaeI YGGCCR 2 cut(s) 424, 526
Eam1104I CTCTTC 2 cut(s) 920, 1151
EarI CTCTTC 2 cut(s) 920, 1151
EciI GGCGGA 2 cut(s) 346, 394
Ecl136II GAGCTC 1 cut(s) 611
Eco130I CCWWGG 1 cut(s) 538
Eco24I GRGCYC 2 cut(s) 206, 613
Eco31I GGTCTC 2 cut(s) 432, 578
Eco47III AGCGCT 1 cut(s) 897
Eco53kI GAGCTC 1 cut(s) 611
Eco72I CACGTG 1 cut(s) 969
Eco88I CYCGRG 3 cut(s) 95, 747, 853
EcoICRI GAGCTC 1 cut(s) 611
EcoT14I CCWWGG 1 cut(s) 538
EcoT38I GRGCYC 2 cut(s) 206, 613
ErhI CCWWGG 1 cut(s) 538
FaeI CATG 2 cut(s) 11, 276
FaiI YATR 7 cut(s) 9, 137, 274, 435, 711, 760, 1176
FaqI GGGAC 2 cut(s) 139, 871
FatI CATG 2 cut(s) 7, 272
FbaI TGATCA 1 cut(s) 864
FblI GTMKAC 1 cut(s) 1044
Fnu4HI GCNGC 6 cut(s) 244, 360, 378, 523, 526, 899
FriOI GRGCYC 2 cut(s) 206, 613
Fsp4HI GCNGC 6 cut(s) 244, 360, 378, 523, 526, 899
FspBI CTAG 1 cut(s) 393
GlaI GCGC 2 cut(s) 872, 897
GluI GCNGC 6 cut(s) 244, 360, 378, 523, 526, 899
GsaI CCCAGC 1 cut(s) 881
HaeII RGCGCY 1 cut(s) 899
HaeIII GGCC 4 cut(s) 5, 426, 528, 768
HapII CCGG 5 cut(s) 240, 384, 561, 666, 1107
HgaI GACGC 2 cut(s) 268, 1061
HhaI GCGC 2 cut(s) 873, 898
Hin1II CATG 2 cut(s) 11, 276
Hin6I GCGC 2 cut(s) 871, 896
HinP1I GCGC 2 cut(s) 871, 896
HincII GTYRAC 3 cut(s) 102, 480, 803
HindII GTYRAC 3 cut(s) 102, 480, 803
HinfI GANTC 7 cut(s) 98, 146, 627, 755, 799, 851, 987
HpaI GTTAAC 1 cut(s) 480
HpaII CCGG 5 cut(s) 240, 384, 561, 666, 1107
HphI GGTGA 2 cut(s) 198, 727
Hpy166II GTNNAC 8 cut(s) 102, 421, 480, 726, 803, 891, 1045, 1051
Hpy188I TCNGA 3 cut(s) 615, 691, 773
Hpy188III TCNNGA 7 cut(s) 55, 174, 705, 747, 837, 914, 1132
Hpy8I GTNNAC 8 cut(s) 102, 421, 480, 726, 803, 891, 1045, 1051
HpyAV CCTTC 1 cut(s) 327
HpyCH4III ACNGT 5 cut(s) 40, 432, 454, 862, 1042
HpyCH4IV ACGT 5 cut(s) 700, 968, 1047, 1146, 1179
HpyCH4V TGCA 4 cut(s) 11, 351, 377, 1165
HpyF10VI GCNNNNNNNGC 4 cut(s) 223, 666, 675, 893
HpyF3I CTNAG 4 cut(s) 612, 674, 679, 942
HpySE526I ACGT 5 cut(s) 700, 968, 1047, 1146, 1179
Hsp92II CATG 2 cut(s) 11, 276
HspAI GCGC 2 cut(s) 871, 896
KpnI GGTACC 1 cut(s) 934
Ksp22I TGATCA 1 cut(s) 864
KspAI GTTAAC 1 cut(s) 480
Kzo9I GATC 6 cut(s) 51, 301, 443, 447, 588, 864
LmnI GCTCC 1 cut(s) 665
Lsp1109I GCAGC 4 cut(s) 255, 389, 509, 885
LweI GCATC 1 cut(s) 798
MaeI CTAG 1 cut(s) 393
MaeII ACGT 5 cut(s) 700, 968, 1047, 1146, 1179
MaeIII GTNAC 4 cut(s) 40, 287, 971, 1036
MalI GATC 6 cut(s) 53, 303, 445, 449, 590, 866
MbiI CCGCTC 1 cut(s) 733
MboI GATC 6 cut(s) 51, 301, 443, 447, 588, 864
MboII GAAGA 6 cut(s) 301, 508, 622, 937, 945, 1168
MfeI CAATTG 1 cut(s) 397
MflI RGATCY 2 cut(s) 301, 588
MhlI GDGCHC 2 cut(s) 206, 613
MluCI AATT 5 cut(s) 16, 138, 397, 810, 843
MlyI GAGTC 4 cut(s) 107, 764, 808, 845
MmeI TCCRAC 3 cut(s) 363, 714, 1177
MseI TTAA 3 cut(s) 479, 504, 549
MspI CCGG 5 cut(s) 240, 384, 561, 666, 1107
MunI CAATTG 1 cut(s) 397
MvnI CGCG 2 cut(s) 362, 871
MwoI GCNNNNNNNGC 4 cut(s) 223, 666, 675, 893
NdeII GATC 6 cut(s) 51, 301, 443, 447, 588, 864
NheI GCTAGC 1 cut(s) 392
NlaIII CATG 2 cut(s) 11, 276
NlaIV GGNNCC 1 cut(s) 932
PaeR7I CTCGAG 1 cut(s) 747
PcsI WCGNNNNNNNCGW 1 cut(s) 827
PfeI GAWTC 3 cut(s) 146, 627, 987
PkrI GCNGC 6 cut(s) 245, 361, 379, 524, 527, 900
Ple19I CGATCG 1 cut(s) 446
PleI GAGTC 4 cut(s) 106, 763, 807, 845
PmaCI CACGTG 1 cut(s) 969
PmlI CACGTG 1 cut(s) 969
PpsI GAGTC 4 cut(s) 106, 763, 807, 845
Ppu21I YACGTR 1 cut(s) 969
Psp124BI GAGCTC 1 cut(s) 613
Psp1406I AACGTT 1 cut(s) 1146
PspCI CACGTG 1 cut(s) 969
PspFI CCCAGC 1 cut(s) 877
PspN4I GGNNCC 1 cut(s) 932
PspPI GGNCC 1 cut(s) 4
PstNI CAGNNNCTG 1 cut(s) 884
PsuI RGATCY 2 cut(s) 301, 588
PvuI CGATCG 1 cut(s) 446
RsaI GTAC 4 cut(s) 165, 620, 890, 932
RsaNI GTAC 4 cut(s) 164, 619, 889, 931
SacI GAGCTC 1 cut(s) 613
SaqAI TTAA 3 cut(s) 479, 504, 549
SatI GCNGC 6 cut(s) 244, 360, 378, 523, 526, 899
Sau3AI GATC 6 cut(s) 51, 301, 443, 447, 588, 864
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 4 cut(s) 107, 764, 808, 845
SduI GDGCHC 2 cut(s) 206, 613
SfaNI GCATC 1 cut(s) 798
Sfr274I CTCGAG 1 cut(s) 747
SlaI CTCGAG 1 cut(s) 747
SmlI CTYRAG 2 cut(s) 79, 747
SmoI CTYRAG 2 cut(s) 79, 747
Sse9I AATT 5 cut(s) 16, 138, 397, 810, 843
SsiI CCGC 6 cut(s) 357, 360, 405, 525, 731, 901
SspI AATATT 1 cut(s) 1065
SspMI CTAG 1 cut(s) 393
SstI GAGCTC 1 cut(s) 613
StyI CCWWGG 1 cut(s) 538
TaaI ACNGT 5 cut(s) 40, 432, 454, 862, 1042
TaiI ACGT 5 cut(s) 703, 971, 1050, 1149, 1182
TaqI TCGA 9 cut(s) 234, 312, 446, 580, 748, 830, 849, 913, 947
TaqII GACCGA 3 cut(s) 456, 560, 566
TasI AATT 5 cut(s) 16, 138, 397, 810, 843
TatI WGTACW 1 cut(s) 618
TauI GCSGC 2 cut(s) 362, 528
TfiI GAWTC 3 cut(s) 146, 627, 987
Tru1I TTAA 3 cut(s) 479, 504, 549
Tru9I TTAA 3 cut(s) 479, 504, 549
TscAI CASTG 4 cut(s) 196, 269, 889, 988
TseI GCWGC 4 cut(s) 243, 377, 522, 898
TspDTI ATGAA 2 cut(s) 74, 938
TspRI CASTG 4 cut(s) 196, 269, 889, 988
XcmI CCANNNNNNNNNTGG 1 cut(s) 164
XhoI CTCGAG 1 cut(s) 747
XmiI GTMKAC 1 cut(s) 1044
XspI CTAG 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.