RLG00000009072

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000845
Physical Location & Seq
Reverse (-)
46049521 .. 46052356
2836 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009072

Sequence Viewer

Length: 1479 bp
ATGGCAGATATTATTAGTCGTAGCTCCTCCTCCTCCTCCTCCTCCTCCTCCTTCCCCCTCAGTATGAGGAATTTCATCGGTTTGTCTCTACTTCTATGCCTGACTCTCTGCTCTTTGGACAAAGGCTTTGCCTTGGCAGAAGCAGATACCCACACAGTTGATCTCCGCTCTCTACTACCAGCAACGACCTGCAGCTCCTCCACCAAAGGTAATAAGAGGAAGGCATCTCTTGAAGTGGTACACAAGCATGGGCCATGCTCTCATCTCCCTCAACACAAAGCGACTGTTAGGAATCACACTCAAATTCTAGAGCAAGACCAAGTGCGAGTCAACTCAATCAACTCCCAGATTTCCAAGAAATTGAACGGCAACGAGGATCTAACCCAATCGGAGGCTACCACCCTCCCGGCCCGGTGGGCTACCAGCCAAGGTGCAAGTAACTACGTGGTGACGGTGGGATTGGGCACACCTAACAAACAACTTTCGCTCGAATTTGACACCGGAAGCTATCTCACTTGGACTCAATGTCAACCTTGTGCTGGCTCTTGCTACCGACAAAAGGAGCCCATTTTCGATCCCTTGGTCTCCAGCTCATATACCAACATTTCCTGTAATAGCACGGATTGCATCCAACTCAGGACAACTACGAAATTCAACAAATGTTTAGTAGACACATGCCTATACGCCGTAGGGTACGGAGACTCATCCTACACCATTGGATTATTCGCCACAGACAAGCTGACCTTAACTCCAAAAGACATGTTCGATGGGTTCCTCTTTGGCTGCGGCCAACAAAACGAAGGCTTCATCAGTGGCTCGGCCGGTATACTTGGTCTCTCCCGCAACAAGTTCTCCATCGTTGAACAAACCGCACAAAAGTACAACCGCTTCTTCTCCTACTGTCTGCCACCAACCGCAAGCTCTACTGGTTACCTCAGCTTCGGGGCACCCGATGGAGCTTCCAATGCTGCTGTAAAATACACACGGCTCACTAATTTATCCTCGAACTCAGTCTTTTACGGCATAGACTTGGTTGGCATCAATGTGAATGGACAAAAGCTCTCAATTCCTGCTTCGGTGTTTTCATCCTCGGGGACGATCATAGACTCCGGGACAGTCATCTCGAGGTTGCCGTCCACAGCGTATACCGCTTTGCGGGATGCGTTTCGGGCAGCGATGACAAGCTATCCGTTAGTGCCAGGACCGAGTAAGCTCCTAGATACATGCTACGACCTTAGTAGCTATCCAAAGGTGACGTATCCGAAAATAGAGTTTGTGTTTGGCGATGGGGTTACGTTGGAATTGGACGCAACCGGAATTGTCTACTTTTTCAATGGTTTGAAGCAGGTTTGCTTGGCTTTTGCTGGGAATGATGATCCTAATGACATTGCGATTTTCGGGAATACTCAGCAAAAGAATATGGAGGTGTTGTATGACGTTGCCGGAGGGAGAGTCGGATTTGCTCCTGGTGGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

493

Amino Acids

52.86

Weight (kDa)

7.41

Isoelectric Point (pI)

34.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 147 - 489 3e-22 Eukaryotic aspartyl protease
TAXi_N PF14543 148 - 315 3e-49 Xylanase inhibitor N-terminal
TAXi_C PF14541 340 - 488 5.2e-29 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 197, 1336
AccB1I GGYRCC 1 cut(s) 946
AccBSI CCGCTC 1 cut(s) 168
AccI GTMKAC 4 cut(s) 669, 826, 1145, 1323
AciI CCGC 8 cut(s) 166, 786, 841, 870, 886, 915, 1149, 1156
AclWI GGATC 3 cut(s) 384, 569, 1370
AcoI YGGCCR 2 cut(s) 787, 819
AcsI RAATTY 4 cut(s) 70, 303, 491, 650
AfaI GTAC 3 cut(s) 240, 695, 881
AfiI CCNNNNNNNGG 4 cut(s) 391, 539, 559, 1155
AflIII ACRYGT 1 cut(s) 759
AgsI TTSAA 6 cut(s) 233, 364, 655, 863, 1333, 1342
AhdI GACNNNNNGTC 1 cut(s) 525
AjnI CCWGG 2 cut(s) 1198, 1465
Alw26I GTCTC 4 cut(s) 90, 589, 693, 839
AlwI GGATC 3 cut(s) 384, 569, 1370
Ama87I CYCGRG 2 cut(s) 1090, 1123
AoxI GGCC 4 cut(s) 251, 408, 787, 819
ApeKI GCWGC 4 cut(s) 192, 783, 968, 1172
ApoI RAATTY 4 cut(s) 70, 303, 491, 650
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
AspS9I GGNCC 3 cut(s) 251, 409, 1202
AsuC2I CCSGG 3 cut(s) 407, 412, 1111
AsuHPI GGTGA 2 cut(s) 460, 1264
AvaI CYCGRG 2 cut(s) 1090, 1123
AvaII GGWCC 1 cut(s) 1202
BaeGI GKGCMC 2 cut(s) 467, 949
BanI GGYRCC 1 cut(s) 946
BanII GRGCYC 1 cut(s) 567
BarI GAAGNNNNNNTAC 2 cut(s) 872, 904
BbvCI CCTCAGC 1 cut(s) 935
BbvI GCAGC 4 cut(s) 204, 770, 955, 1184
BccI CCATC 4 cut(s) 761, 863, 947, 1280
BceAI ACGGC 5 cut(s) 382, 671, 1001, 1036, 1117
BciT130I CCWGG 2 cut(s) 1200, 1467
BciVI GTATCC 1 cut(s) 1269
BcnI CCSGG 3 cut(s) 407, 412, 1111
BcoDI GTCTC 4 cut(s) 90, 589, 693, 839
BfaI CTAG 2 cut(s) 308, 1217
BfmI CTRYAG 1 cut(s) 190
BfuAI ACCTGC 2 cut(s) 197, 1336
BfuI GTATCC 1 cut(s) 1269
BglI GCCNNNNNGGC 1 cut(s) 416
BisI GCNGC 5 cut(s) 193, 784, 787, 969, 1173
BlsI GCNGC 5 cut(s) 194, 785, 788, 970, 1174
Bme1390I CCNGG 5 cut(s) 407, 412, 1111, 1200, 1467
Bme18I GGWCC 1 cut(s) 1202
BmeRI GACNNNNNGTC 1 cut(s) 525
BmeT110I CYCGRG 2 cut(s) 1090, 1123
BmgT120I GGNCC 3 cut(s) 251, 409, 1202
BmiI GGNNCC 3 cut(s) 564, 773, 948
BmrFI CCNGG 5 cut(s) 407, 412, 1111, 1200, 1467
BmsI GCATC 4 cut(s) 233, 636, 1047, 1150
BpmI CTGGAG 1 cut(s) 571
Bpu10I CCTNAGC 1 cut(s) 935
BpuMI CCSGG 3 cut(s) 407, 412, 1111
BsaAI YACGTR 1 cut(s) 445
BsaI GGTCTC 2 cut(s) 589, 839
BsaJI CCNNGG 4 cut(s) 132, 427, 579, 1089
BsaWI WCCGGW 2 cut(s) 500, 1313
BsaXI ACNNNNNCTCC 8 cut(s) 733, 763, 836, 866, 1415, 1437, 1445, 1467
Bsc4I CCNNNNNNNGG 4 cut(s) 391, 539, 559, 1155
Bse118I RCCGGY 1 cut(s) 821
Bse1I ACTGG 1 cut(s) 931
Bse3DI GCAATG 1 cut(s) 1386
BseBI CCWGG 2 cut(s) 1200, 1467
BseDI CCNNGG 4 cut(s) 132, 427, 579, 1089
BseGI GGATG 4 cut(s) 627, 704, 1085, 1165
BseLI CCNNNNNNNGG 4 cut(s) 391, 539, 559, 1155
BseMI GCAATG 1 cut(s) 1386
BseMII CTCAG 5 cut(s) 73, 649, 949, 1023, 1421
BseNI ACTGG 1 cut(s) 931
BseRI GAGGAG 9 cut(s) 16, 19, 22, 25, 28, 31, 34, 37, 187
BseSI GKGCMC 2 cut(s) 467, 949
BseX3I CGGCCG 1 cut(s) 819
BseXI GCAGC 4 cut(s) 204, 770, 955, 1184
BseYI CCCAGC 1 cut(s) 1364
Bsh1285I CGRYCG 1 cut(s) 822
BshFI GGCC 4 cut(s) 253, 410, 789, 821
BshNI GGYRCC 1 cut(s) 946
BsiEI CGRYCG 1 cut(s) 822
BsiHKCI CYCGRG 2 cut(s) 1090, 1123
BsiSI CCGG 7 cut(s) 407, 412, 501, 822, 1110, 1314, 1443
BslFI GGGAC 2 cut(s) 1108, 1126
BslI CCNNNNNNNGG 4 cut(s) 391, 539, 559, 1155
BsmAI GTCTC 4 cut(s) 90, 589, 693, 839
BsmFI GGGAC 2 cut(s) 1108, 1126
BsnI GGCC 4 cut(s) 253, 410, 789, 821
Bso31I GGTCTC 2 cut(s) 589, 839
BsoBI CYCGRG 2 cut(s) 1090, 1123
Bsp1286I GDGCHC 3 cut(s) 467, 567, 949
Bsp143I GATC 5 cut(s) 160, 376, 574, 1098, 1375
BspACI CCGC 8 cut(s) 166, 786, 841, 870, 886, 915, 1149, 1156
BspANI GGCC 4 cut(s) 253, 410, 789, 821
BspCNI CTCAG 5 cut(s) 72, 648, 948, 1022, 1420
BspLI GGNNCC 3 cut(s) 564, 773, 948
BspMAI CTGCAG 1 cut(s) 194
BspMI ACCTGC 2 cut(s) 197, 1336
BspPI GGATC 3 cut(s) 384, 569, 1370
BspT107I GGYRCC 1 cut(s) 946
BspTNI GGTCTC 2 cut(s) 589, 839
BsrBI CCGCTC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 1386
BsrFI RCCGGY 1 cut(s) 821
BsrI ACTGG 1 cut(s) 931
BssAI RCCGGY 1 cut(s) 821
BssECI CCNNGG 4 cut(s) 132, 427, 579, 1089
BssMI GATC 5 cut(s) 160, 376, 574, 1098, 1375
BssNAI GTATAC 2 cut(s) 827, 1146
BssT1I CCWWGG 3 cut(s) 132, 427, 579
Bst1107I GTATAC 2 cut(s) 827, 1146
Bst2UI CCWGG 2 cut(s) 1200, 1467
Bst4CI ACNGT 5 cut(s) 157, 286, 454, 902, 1117
BstAPI GCANNNNNTGC 1 cut(s) 624
BstBAI YACGTR 1 cut(s) 445
BstC8I GCNNGC 2 cut(s) 541, 919
BstDEI CTNAG 6 cut(s) 59, 635, 935, 1009, 1235, 1407
BstEII GGTNACC 1 cut(s) 929
BstF5I GGATG 4 cut(s) 627, 704, 1085, 1165
BstKTI GATC 5 cut(s) 163, 379, 577, 1101, 1378
BstMAI GTCTC 4 cut(s) 90, 589, 693, 839
BstMBI GATC 5 cut(s) 160, 376, 574, 1098, 1375
BstMCI CGRYCG 1 cut(s) 822
BstMWI GCNNNNNNNGC 5 cut(s) 416, 624, 965, 1148, 1169
BstNI CCWGG 2 cut(s) 1200, 1467
BstNSI RCATGY 3 cut(s) 678, 763, 1227
BstPI GGTNACC 1 cut(s) 929
BstSCI CCNGG 5 cut(s) 405, 410, 1109, 1198, 1465
BstSFI CTRYAG 1 cut(s) 190
BstSLI GKGCMC 2 cut(s) 467, 949
BstV1I GCAGC 4 cut(s) 204, 770, 955, 1184
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BstZ17I GTATAC 2 cut(s) 827, 1146
BstZI CGGCCG 1 cut(s) 819
BsuI GTATCC 1 cut(s) 1269
BsuRI GGCC 4 cut(s) 253, 410, 789, 821
BtgZI GCGATG 2 cut(s) 1190, 1299
BtsCI GGATG 4 cut(s) 627, 704, 1085, 1165
BtsIMutI CAGTG 1 cut(s) 817
BveI ACCTGC 2 cut(s) 197, 1336
Cac8I GCNNGC 2 cut(s) 541, 919
Cfr10I RCCGGY 1 cut(s) 821
Cfr13I GGNCC 3 cut(s) 251, 409, 1202
CseI GACGC 1 cut(s) 1316
Csp6I GTAC 3 cut(s) 239, 694, 880
CviAII CATG 5 cut(s) 248, 255, 675, 760, 1224
CviQI GTAC 3 cut(s) 239, 694, 880
DdeI CTNAG 6 cut(s) 59, 635, 935, 1009, 1235, 1407
DpnI GATC 5 cut(s) 162, 378, 576, 1100, 1377
DpnII GATC 5 cut(s) 160, 376, 574, 1098, 1375
DriI GACNNNNNGTC 1 cut(s) 525
EaeI YGGCCR 2 cut(s) 787, 819
EagI CGGCCG 1 cut(s) 819
Eam1105I GACNNNNNGTC 1 cut(s) 525
EclXI CGGCCG 1 cut(s) 819
Eco130I CCWWGG 3 cut(s) 132, 427, 579
Eco24I GRGCYC 1 cut(s) 567
Eco31I GGTCTC 2 cut(s) 589, 839
Eco47I GGWCC 1 cut(s) 1202
Eco52I CGGCCG 1 cut(s) 819
Eco88I CYCGRG 2 cut(s) 1090, 1123
Eco91I GGTNACC 1 cut(s) 929
EcoO65I GGTNACC 1 cut(s) 929
EcoRII CCWGG 2 cut(s) 1198, 1465
EcoT14I CCWWGG 3 cut(s) 132, 427, 579
EcoT38I GRGCYC 1 cut(s) 567
ErhI CCWWGG 3 cut(s) 132, 427, 579
FaeI CATG 5 cut(s) 251, 258, 678, 763, 1227
FalI AAGNNNNNCTT 2 cut(s) 728, 760
FaqI GGGAC 2 cut(s) 1108, 1126
FatI CATG 5 cut(s) 247, 254, 674, 759, 1223
FauI CCCGC 2 cut(s) 848, 1149
FblI GTMKAC 4 cut(s) 669, 826, 1145, 1323
Fnu4HI GCNGC 5 cut(s) 193, 784, 787, 969, 1173
FokI GGATG 4 cut(s) 614, 691, 1072, 1172
FriOI GRGCYC 1 cut(s) 567
Fsp4HI GCNGC 5 cut(s) 193, 784, 787, 969, 1173
FspBI CTAG 2 cut(s) 308, 1217
GluI GCNGC 5 cut(s) 193, 784, 787, 969, 1173
GsaI CCCAGC 1 cut(s) 1368
GsuI CTGGAG 1 cut(s) 571
HaeIII GGCC 4 cut(s) 253, 410, 789, 821
HapII CCGG 7 cut(s) 407, 412, 501, 822, 1110, 1314, 1443
HgaI GACGC 1 cut(s) 1316
Hin1II CATG 5 cut(s) 251, 258, 678, 763, 1227
HincII GTYRAC 2 cut(s) 331, 530
HindII GTYRAC 2 cut(s) 331, 530
HinfI GANTC 7 cut(s) 103, 292, 327, 520, 701, 1106, 1452
HpaII CCGG 7 cut(s) 407, 412, 501, 822, 1110, 1314, 1443
HphI GGTGA 2 cut(s) 460, 1264
Hpy166II GTNNAC 8 cut(s) 241, 331, 530, 670, 827, 1137, 1146, 1324
Hpy188I TCNGA 3 cut(s) 391, 1263, 1457
Hpy188III TCNNGA 5 cut(s) 230, 308, 637, 1123, 1399
Hpy8I GTNNAC 8 cut(s) 241, 331, 530, 670, 827, 1137, 1146, 1324
HpyAV CCTTC 3 cut(s) 61, 214, 794
HpyCH4III ACNGT 5 cut(s) 157, 286, 454, 902, 1117
HpyCH4IV ACGT 4 cut(s) 444, 1256, 1295, 1437
HpyCH4V TGCA 3 cut(s) 192, 434, 627
HpyF10VI GCNNNNNNNGC 5 cut(s) 416, 624, 965, 1148, 1169
HpyF3I CTNAG 6 cut(s) 59, 635, 935, 1009, 1235, 1407
HpySE526I ACGT 4 cut(s) 444, 1256, 1295, 1437
Hsp92II CATG 5 cut(s) 251, 258, 678, 763, 1227
Kzo9I GATC 5 cut(s) 160, 376, 574, 1098, 1375
LmnI GCTCC 6 cut(s) 29, 200, 562, 956, 1218, 1468
Lsp1109I GCAGC 4 cut(s) 204, 770, 955, 1184
LweI GCATC 4 cut(s) 233, 636, 1047, 1150
MaeI CTAG 2 cut(s) 308, 1217
MaeII ACGT 4 cut(s) 444, 1256, 1295, 1437
MaeIII GTNAC 5 cut(s) 437, 448, 929, 1252, 1291
MalI GATC 5 cut(s) 162, 378, 576, 1100, 1377
MbiI CCGCTC 1 cut(s) 168
MboI GATC 5 cut(s) 160, 376, 574, 1098, 1375
MboII GAAGA 1 cut(s) 883
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 3 cut(s) 467, 567, 949
MluCI AATT 9 cut(s) 70, 303, 359, 491, 650, 994, 1065, 1301, 1317
MlyI GAGTC 6 cut(s) 97, 336, 514, 695, 1100, 1461
MmeI TCCRAC 3 cut(s) 655, 1278, 1435
MseI TTAA 1 cut(s) 746
MslI CAYNNNNRTG 2 cut(s) 246, 1043
MspI CCGG 7 cut(s) 407, 412, 501, 822, 1110, 1314, 1443
MspR9I CCNGG 5 cut(s) 407, 412, 1111, 1200, 1467
MvaI CCWGG 2 cut(s) 1200, 1467
MwoI GCNNNNNNNGC 5 cut(s) 416, 624, 965, 1148, 1169
NciI CCSGG 3 cut(s) 407, 412, 1111
NdeII GATC 5 cut(s) 160, 376, 574, 1098, 1375
NlaIII CATG 5 cut(s) 251, 258, 678, 763, 1227
NlaIV GGNNCC 3 cut(s) 564, 773, 948
NmeAIII GCCGAG 1 cut(s) 797
NmuCI GTSAC 2 cut(s) 448, 1252
NspI RCATGY 3 cut(s) 678, 763, 1227
PaeR7I CTCGAG 1 cut(s) 1123
PciI ACATGT 1 cut(s) 759
PcsI WCGNNNNNNNCGW 2 cut(s) 948, 1130
PfeI GAWTC 1 cut(s) 292
PfoI TCCNGGA 1 cut(s) 1109
PkrI GCNGC 5 cut(s) 194, 785, 788, 970, 1174
PleI GAGTC 6 cut(s) 97, 335, 514, 695, 1100, 1460
PpsI GAGTC 6 cut(s) 97, 335, 514, 695, 1100, 1460
Ppu21I YACGTR 1 cut(s) 445
PscI ACATGT 1 cut(s) 759
Psp6I CCWGG 2 cut(s) 1198, 1465
PspEI GGTNACC 1 cut(s) 929
PspFI CCCAGC 1 cut(s) 1364
PspGI CCWGG 2 cut(s) 1198, 1465
PspN4I GGNNCC 3 cut(s) 564, 773, 948
PspPI GGNCC 3 cut(s) 251, 409, 1202
PstI CTGCAG 1 cut(s) 194
PsuI RGATCY 1 cut(s) 376
RsaI GTAC 3 cut(s) 240, 695, 881
RsaNI GTAC 3 cut(s) 239, 694, 880
RseI CAYNNNNRTG 2 cut(s) 246, 1043
SaqAI TTAA 1 cut(s) 746
SatI GCNGC 5 cut(s) 193, 784, 787, 969, 1173
Sau3AI GATC 5 cut(s) 160, 376, 574, 1098, 1375
Sau96I GGNCC 3 cut(s) 251, 409, 1202
SchI GAGTC 6 cut(s) 97, 336, 514, 695, 1100, 1461
ScrFI CCNGG 5 cut(s) 407, 412, 1111, 1200, 1467
SduI GDGCHC 3 cut(s) 467, 567, 949
SfaNI GCATC 4 cut(s) 233, 636, 1047, 1150
SfcI CTRYAG 1 cut(s) 190
Sfr274I CTCGAG 1 cut(s) 1123
SinI GGWCC 1 cut(s) 1202
SlaI CTCGAG 1 cut(s) 1123
SmiMI CAYNNNNRTG 2 cut(s) 246, 1043
SmlI CTYRAG 1 cut(s) 1123
SmoI CTYRAG 1 cut(s) 1123
Sse9I AATT 9 cut(s) 70, 303, 359, 491, 650, 994, 1065, 1301, 1317
SsiI CCGC 8 cut(s) 166, 786, 841, 870, 886, 915, 1149, 1156
SspMI CTAG 2 cut(s) 308, 1217
StyD4I CCNGG 5 cut(s) 405, 410, 1109, 1198, 1465
StyI CCWWGG 3 cut(s) 132, 427, 579
TaaI ACNGT 5 cut(s) 157, 286, 454, 902, 1117
TaiI ACGT 4 cut(s) 447, 1259, 1298, 1440
TaqI TCGA 5 cut(s) 489, 573, 765, 1004, 1124
TaqII GACCGA 1 cut(s) 1219
TasI AATT 9 cut(s) 70, 303, 359, 491, 650, 994, 1065, 1301, 1317
TatI WGTACW 1 cut(s) 879
TauI GCSGC 1 cut(s) 789
TfiI GAWTC 1 cut(s) 292
Tru1I TTAA 1 cut(s) 746
Tru9I TTAA 1 cut(s) 746
TscAI CASTG 1 cut(s) 817
TseFI GTSAC 2 cut(s) 448, 1252
TseI GCWGC 4 cut(s) 192, 783, 968, 1172
Tsp45I GTSAC 2 cut(s) 448, 1252
TspDTI ATGAA 3 cut(s) 64, 796, 1074
TspGWI ACGGA 3 cut(s) 635, 711, 1179
TspRI CASTG 1 cut(s) 817
VpaK11BI GGWCC 1 cut(s) 1202
XapI RAATTY 4 cut(s) 70, 303, 491, 650
XbaI TCTAGA 1 cut(s) 307
XceI RCATGY 3 cut(s) 678, 763, 1227
XhoI CTCGAG 1 cut(s) 1123
XmiI GTMKAC 4 cut(s) 669, 826, 1145, 1323
XspI CTAG 2 cut(s) 308, 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.