pycom08g04220

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000845
Physical Location & Seq
Reverse (-)
3248482 .. 3250252
1771 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g04220.6

Sequence Viewer

Length: 1212 bp
ATGCTTGCATGTTGTCTCTTCAATCTTAATGCATCGCACTCTGCTTCGTTTTACACCTGCATTCATGGAGTAATTGGAATGGTGCATGAATATTACTCTTCCAGCTACAAATACACACACAGAAGAGCATCAGTACTAAAAGTGGTACACAGGCATGGGCCATGCAATCAACTCCGCCAACGTAAACCAAAAAGCAGTAATACTGATCAGGATGAGGACCTCACACAAATCCTCGAAATCCCTGAAGGACGACTCAAACAATCTGATGCCACCGTCATCCCCGCCCAGTCAGGCAACCTTAGGGGGGCTATGGCGACTACATGGTCAGCATCGCGACATCTCTTGGACACAATGCCAACCTTGTGTTCGGTCTTGTTACCCACAGAAAGACCCATATTCGACCCCTCTCTCTCTACCTCTTACTCCAACATCTCCTGCAACTCTGCCGCGTGTTCTCAGCTCGCAGCCTCCGGTGTTGCTAGCAGTTGTTCCGCCTCCACCTGTGTTTACGGCGAACGGTTTGGAGACGGCTCCTTCACAGTCGGCTTCTGGGCCAAAGAGAAGCTAACTCTGACTTCAACAGACTTTTTTAACGGTTTTATCTTCAGATGCGGCCAGAACAACCAAGGCTTATTTAGGGGCATCGCCGGTTTACTCGGTCTCGGCCGAAGCAATATCTCCATAGTCGAACAAACCGCCGACAAATATAAACGGTTCTTGTCCTACTGCCTCCCATCCACTTCAAGCTCTACCGGTTACCTCAGCTTCGGCAAAGCTGGTGGCAAAGGTTCGAGAAAGACCGTCAAATTCACACCGCTGACCACATTATCTCAAGGTCCAGGGGTTTACGGCCTCAGACTGGTTGGCATCAACGTCGGCGGGCGGATGGTGATCACGCGCCTCCCGGCCACGGCATACGCCGCACTGCGGGGAGCATTTCAGGCACCAATGAAGAGGTACCCGTCGGCTAACTCGACTTCCACCTTGCTGGACACGTGTTACGATTTCAGCGGATTCCAAACGATGTCGTATCCAAAGATAGGATTTGTGTTTAGCGGCGGGGTTACGGTGGACGTTGACGCTGCCGGGATATTTTTTCAGCAGAGTGCTCCGCAGTTTTGCTTGGCTTTTGCAGGAAACAAGGATGATAGGGACATTGGGATTATTGGGAATGGGAAAGGTGGGATTTGCCCCCCTGCTGGATGCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

404

Amino Acids

43.18

Weight (kDa)

9.16

Isoelectric Point (pI)

37.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 65
AasI GACNNNNNNGTC 1 cut(s) 322
Acc36I ACCTGC 1 cut(s) 65
Acc65I GGTACC 1 cut(s) 957
AccB1I GGYRCC 2 cut(s) 943, 957
AccII CGCG 3 cut(s) 334, 449, 898
AcoI YGGCCR 3 cut(s) 613, 664, 906
AcsI RAATTY 1 cut(s) 806
AcuI CTGAAG 2 cut(s) 264, 589
AcvI CACGTG 1 cut(s) 996
AfaI GTAC 3 cut(s) 135, 147, 959
AfiI CCNNNNNNNGG 6 cut(s) 304, 859, 910, 927, 1040, 1199
AflIII ACRYGT 2 cut(s) 993, 995
AgeI ACCGGT 1 cut(s) 752
AgsI TTSAA 3 cut(s) 22, 579, 744
AjnI CCWGG 1 cut(s) 838
AjuI GAANNNNNNNTTGG 2 cut(s) 349, 381
AluBI AGCT 6 cut(s) 105, 460, 565, 747, 765, 776
AluI AGCT 6 cut(s) 105, 460, 565, 747, 765, 776
Alw21I GWGCWC 1 cut(s) 1111
Alw26I GTCTC 3 cut(s) 20, 519, 665
AoxI GGCC 6 cut(s) 158, 552, 613, 664, 850, 906
ApeKI GCWGC 2 cut(s) 464, 1082
ApoI RAATTY 1 cut(s) 806
AsiGI ACCGGT 1 cut(s) 752
Asp718I GGTACC 1 cut(s) 957
AspLEI GCGC 1 cut(s) 900
AspS9I GGNCC 4 cut(s) 158, 217, 552, 836
AsuC2I CCSGG 2 cut(s) 905, 1087
AsuHPI GGTGA 1 cut(s) 901
AsuNHI GCTAGC 1 cut(s) 479
AvaII GGWCC 2 cut(s) 217, 836
AxyI CCTNAGG 1 cut(s) 299
BanI GGYRCC 2 cut(s) 943, 957
BbrPI CACGTG 1 cut(s) 996
Bbv12I GWGCWC 1 cut(s) 1111
BbvCI CCTCAGC 1 cut(s) 761
BbvI GCAGC 2 cut(s) 476, 1069
BccI CCATC 2 cut(s) 742, 880
BceAI ACGGC 4 cut(s) 526, 544, 865, 927
BcgI CGANNNNNNTGC 2 cut(s) 856, 890
BciT130I CCWGG 1 cut(s) 840
BciVI GTATCC 1 cut(s) 1041
BclI TGATCA 2 cut(s) 205, 891
BcnI CCSGG 2 cut(s) 905, 1087
BcoDI GTCTC 3 cut(s) 20, 519, 665
BfaI CTAG 1 cut(s) 480
BfuAI ACCTGC 1 cut(s) 65
BfuI GTATCC 1 cut(s) 1041
BisI GCNGC 6 cut(s) 447, 465, 613, 921, 1057, 1083
BlsI GCNGC 6 cut(s) 448, 466, 614, 922, 1058, 1084
BmcAI AGTACT 1 cut(s) 135
Bme1390I CCNGG 3 cut(s) 840, 905, 1087
Bme18I GGWCC 2 cut(s) 217, 836
BmgT120I GGNCC 4 cut(s) 158, 217, 552, 836
BmiI GGNNCC 3 cut(s) 532, 945, 959
BmrFI CCNGG 3 cut(s) 840, 905, 1087
BmrI ACTGGG 1 cut(s) 280
BmsI GCATC 8 cut(s) 41, 137, 256, 338, 599, 651, 876, 1193
BmtI GCTAGC 1 cut(s) 483
BmuI ACTGGG 1 cut(s) 280
Bpu10I CCTNAGC 1 cut(s) 761
BpuEI CTTGAG 1 cut(s) 816
BpuMI CCSGG 2 cut(s) 905, 1087
BsaAI YACGTR 1 cut(s) 996
BsaBI GATNNNNATC 1 cut(s) 890
BsaI GGTCTC 1 cut(s) 665
BsaJI CCNNGG 3 cut(s) 625, 839, 909
BsaWI WCCGGW 2 cut(s) 470, 752
Bsc4I CCNNNNNNNGG 6 cut(s) 304, 859, 910, 927, 1040, 1199
Bse118I RCCGGY 2 cut(s) 647, 752
Bse1I ACTGG 2 cut(s) 286, 864
Bse21I CCTNAGG 1 cut(s) 299
Bse8I GATNNNNATC 1 cut(s) 890
BseBI CCWGG 1 cut(s) 840
BseDI CCNNGG 3 cut(s) 625, 839, 909
BseGI GGATG 6 cut(s) 217, 276, 734, 891, 1150, 1208
BseJI GATNNNNATC 1 cut(s) 890
BseLI CCNNNNNNNGG 6 cut(s) 304, 859, 910, 927, 1040, 1199
BseMII CTCAG 3 cut(s) 470, 775, 868
BseNI ACTGG 2 cut(s) 286, 864
BseX3I CGGCCG 1 cut(s) 664
BseXI GCAGC 2 cut(s) 476, 1069
Bsh1236I CGCG 3 cut(s) 334, 449, 898
Bsh1285I CGRYCG 1 cut(s) 667
BshFI GGCC 6 cut(s) 160, 554, 615, 666, 852, 908
BshNI GGYRCC 2 cut(s) 943, 957
BshTI ACCGGT 1 cut(s) 752
BsiEI CGRYCG 1 cut(s) 667
BsiHKAI GWGCWC 1 cut(s) 1111
BsiSI CCGG 5 cut(s) 471, 648, 753, 905, 1086
BslFI GGGAC 1 cut(s) 1166
BslI CCNNNNNNNGG 6 cut(s) 304, 859, 910, 927, 1040, 1199
BsmAI GTCTC 3 cut(s) 20, 519, 665
BsmBI CGTCTC 1 cut(s) 519
BsmFI GGGAC 1 cut(s) 1166
BsmI GAATGC 1 cut(s) 60
BsnI GGCC 6 cut(s) 160, 554, 615, 666, 852, 908
Bso31I GGTCTC 1 cut(s) 665
Bsp1286I GDGCHC 1 cut(s) 1111
Bsp143I GATC 2 cut(s) 205, 891
Bsp68I TCGCGA 1 cut(s) 334
BspANI GGCC 6 cut(s) 160, 554, 615, 666, 852, 908
BspCNI CTCAG 3 cut(s) 469, 774, 867
BspFNI CGCG 3 cut(s) 334, 449, 898
BspLI GGNNCC 3 cut(s) 532, 945, 959
BspMI ACCTGC 1 cut(s) 65
BspOI GCTAGC 1 cut(s) 483
BspQI GCTCTTC 1 cut(s) 118
BspT107I GGYRCC 2 cut(s) 943, 957
BspTNI GGTCTC 1 cut(s) 665
BsrFI RCCGGY 2 cut(s) 647, 752
BsrI ACTGG 2 cut(s) 286, 864
BssAI RCCGGY 2 cut(s) 647, 752
BssECI CCNNGG 3 cut(s) 625, 839, 909
BssMI GATC 2 cut(s) 205, 891
BssT1I CCWWGG 1 cut(s) 625
Bst2UI CCWGG 1 cut(s) 840
Bst4CI ACNGT 7 cut(s) 274, 519, 541, 596, 714, 802, 1069
Bst6I CTCTTC 4 cut(s) 23, 103, 118, 947
BstBAI YACGTR 1 cut(s) 996
BstC8I GCNNGC 4 cut(s) 6, 462, 481, 881
BstDEI CTNAG 4 cut(s) 299, 456, 761, 854
BstDSI CCRYGG 1 cut(s) 909
BstEII GGTNACC 1 cut(s) 755
BstF5I GGATG 6 cut(s) 217, 276, 734, 891, 1150, 1208
BstFNI CGCG 3 cut(s) 334, 449, 898
BstHHI GCGC 1 cut(s) 900
BstKTI GATC 2 cut(s) 208, 894
BstMAI GTCTC 3 cut(s) 20, 519, 665
BstMBI GATC 2 cut(s) 205, 891
BstMCI CGRYCG 1 cut(s) 667
BstMWI GCNNNNNNNGC 2 cut(s) 920, 941
BstNI CCWGG 1 cut(s) 840
BstNSI RCATGY 1 cut(s) 12
BstPI GGTNACC 1 cut(s) 755
BstSCI CCNGG 3 cut(s) 838, 903, 1085
BstUI CGCG 3 cut(s) 334, 449, 898
BstV1I GCAGC 2 cut(s) 476, 1069
BstXI CCANNNNNNTGG 1 cut(s) 988
BstZI CGGCCG 1 cut(s) 664
Bsu36I CCTNAGG 1 cut(s) 299
BsuI GTATCC 1 cut(s) 1041
BsuRI GGCC 6 cut(s) 160, 554, 615, 666, 852, 908
BtgI CCRYGG 1 cut(s) 909
BtgZI GCGATG 3 cut(s) 18, 315, 628
BtsCI GGATG 6 cut(s) 217, 276, 734, 891, 1150, 1208
BtsI GCAGTG 1 cut(s) 923
BtsIMutI CAGTG 1 cut(s) 923
BtuMI TCGCGA 1 cut(s) 334
BveI ACCTGC 1 cut(s) 65
Cac8I GCNNGC 4 cut(s) 6, 462, 481, 881
CfoI GCGC 1 cut(s) 900
Cfr10I RCCGGY 2 cut(s) 647, 752
Cfr13I GGNCC 4 cut(s) 158, 217, 552, 836
CseI GACGC 1 cut(s) 1088
Csp6I GTAC 3 cut(s) 134, 146, 958
CspAI ACCGGT 1 cut(s) 752
CspCI CAANNNNNGTGG 2 cut(s) 760, 795
CviAII CATG 6 cut(s) 9, 65, 86, 155, 162, 321
CviQI GTAC 3 cut(s) 134, 146, 958
DdeI CTNAG 4 cut(s) 299, 456, 761, 854
DpnI GATC 2 cut(s) 207, 893
DpnII GATC 2 cut(s) 205, 891
DrdI GACNNNNNNGTC 1 cut(s) 322
DseDI GACNNNNNNGTC 1 cut(s) 322
EaeI YGGCCR 3 cut(s) 613, 664, 906
EagI CGGCCG 1 cut(s) 664
Eam1104I CTCTTC 4 cut(s) 23, 103, 118, 947
EarI CTCTTC 4 cut(s) 23, 103, 118, 947
EciI GGCGGA 3 cut(s) 164, 481, 898
EclXI CGGCCG 1 cut(s) 664
Eco130I CCWWGG 1 cut(s) 625
Eco31I GGTCTC 1 cut(s) 665
Eco47I GGWCC 2 cut(s) 217, 836
Eco52I CGGCCG 1 cut(s) 664
Eco57I CTGAAG 2 cut(s) 264, 589
Eco72I CACGTG 1 cut(s) 996
Eco81I CCTNAGG 1 cut(s) 299
Eco91I GGTNACC 1 cut(s) 755
EcoO109I RGGNCCY 1 cut(s) 217
EcoO65I GGTNACC 1 cut(s) 755
EcoRII CCWGG 1 cut(s) 838
EcoT14I CCWWGG 1 cut(s) 625
EcoT22I ATGCAT 1 cut(s) 34
ErhI CCWWGG 1 cut(s) 625
Esp3I CGTCTC 1 cut(s) 519
FaeI CATG 6 cut(s) 12, 68, 89, 158, 165, 324
FaqI GGGAC 1 cut(s) 1166
FatI CATG 6 cut(s) 8, 64, 85, 154, 161, 320
FauI CCCGC 4 cut(s) 289, 872, 921, 1052
FbaI TGATCA 2 cut(s) 205, 891
Fnu4HI GCNGC 6 cut(s) 447, 465, 613, 921, 1057, 1083
FokI GGATG 5 cut(s) 224, 263, 721, 898, 1157
Fsp4HI GCNGC 6 cut(s) 447, 465, 613, 921, 1057, 1083
FspBI CTAG 1 cut(s) 480
GlaI GCGC 1 cut(s) 899
GluI GCNGC 6 cut(s) 447, 465, 613, 921, 1057, 1083
HaeIII GGCC 6 cut(s) 160, 554, 615, 666, 852, 908
HapII CCGG 5 cut(s) 471, 648, 753, 905, 1086
HgaI GACGC 1 cut(s) 1088
HhaI GCGC 1 cut(s) 900
Hin1II CATG 6 cut(s) 12, 68, 89, 158, 165, 324
Hin6I GCGC 1 cut(s) 898
HinP1I GCGC 1 cut(s) 898
HincII GTYRAC 1 cut(s) 1078
HindII GTYRAC 1 cut(s) 1078
HinfI GANTC 2 cut(s) 252, 1014
HpaII CCGG 5 cut(s) 471, 648, 753, 905, 1086
HphI GGTGA 1 cut(s) 901
Hpy166II GTNNAC 7 cut(s) 148, 185, 508, 653, 847, 1072, 1078
Hpy188I TCNGA 4 cut(s) 265, 573, 608, 857
Hpy188III TCNNGA 4 cut(s) 209, 333, 792, 1209
Hpy8I GTNNAC 7 cut(s) 148, 185, 508, 653, 847, 1072, 1078
Hpy99I CGWCG 2 cut(s) 878, 967
HpyAV CCTTC 2 cut(s) 239, 544
HpyCH4III ACNGT 7 cut(s) 274, 519, 541, 596, 714, 802, 1069
HpyCH4IV ACGT 4 cut(s) 181, 873, 995, 1074
HpyCH4V TGCA 7 cut(s) 8, 32, 60, 85, 165, 438, 1133
HpyF10VI GCNNNNNNNGC 2 cut(s) 920, 941
HpyF3I CTNAG 4 cut(s) 299, 456, 761, 854
HpySE526I ACGT 4 cut(s) 181, 873, 995, 1074
Hsp92II CATG 6 cut(s) 12, 68, 89, 158, 165, 324
HspAI GCGC 1 cut(s) 898
KpnI GGTACC 1 cut(s) 961
Ksp22I TGATCA 2 cut(s) 205, 891
Kzo9I GATC 2 cut(s) 205, 891
LguI GCTCTTC 1 cut(s) 118
LmnI GCTCC 4 cut(s) 536, 932, 1114, 1211
Lsp1109I GCAGC 2 cut(s) 476, 1069
LweI GCATC 8 cut(s) 41, 137, 256, 338, 599, 651, 876, 1193
MaeI CTAG 1 cut(s) 480
MaeII ACGT 4 cut(s) 181, 873, 995, 1074
MaeIII GTNAC 4 cut(s) 375, 755, 998, 1063
MalI GATC 2 cut(s) 207, 893
MboI GATC 2 cut(s) 205, 891
MboII GAAGA 5 cut(s) 10, 90, 135, 595, 964
MhlI GDGCHC 1 cut(s) 1111
MluCI AATT 2 cut(s) 72, 806
MlyI GAGTC 1 cut(s) 246
MmeI TCCRAC 1 cut(s) 450
Mph1103I ATGCAT 1 cut(s) 34
MseI TTAA 2 cut(s) 27, 591
MslI CAYNNNNRTG 1 cut(s) 153
MspA1I CMGCKG 2 cut(s) 817, 1011
MspI CCGG 5 cut(s) 471, 648, 753, 905, 1086
MspR9I CCNGG 3 cut(s) 840, 905, 1087
Mva1269I GAATGC 1 cut(s) 60
MvaI CCWGG 1 cut(s) 840
MvnI CGCG 3 cut(s) 334, 449, 898
MwoI GCNNNNNNNGC 2 cut(s) 920, 941
NciI CCSGG 2 cut(s) 905, 1087
NdeII GATC 2 cut(s) 205, 891
NheI GCTAGC 1 cut(s) 479
NlaIII CATG 6 cut(s) 12, 68, 89, 158, 165, 324
NlaIV GGNNCC 3 cut(s) 532, 945, 959
NmeAIII GCCGAG 1 cut(s) 642
NruI TCGCGA 1 cut(s) 334
NsiI ATGCAT 1 cut(s) 34
NspI RCATGY 1 cut(s) 12
PaqCI CACCTGC 1 cut(s) 65
PciSI GCTCTTC 1 cut(s) 118
PcsI WCGNNNNNNNCGW 1 cut(s) 971
PctI GAATGC 1 cut(s) 60
PfeI GAWTC 1 cut(s) 1014
PinAI ACCGGT 1 cut(s) 752
PkrI GCNGC 6 cut(s) 448, 466, 614, 922, 1058, 1084
PleI GAGTC 1 cut(s) 246
PmaCI CACGTG 1 cut(s) 996
PmlI CACGTG 1 cut(s) 996
PpsI GAGTC 1 cut(s) 246
Ppu21I YACGTR 1 cut(s) 996
PpuMI RGGWCCY 1 cut(s) 217
Psp5II RGGWCCY 1 cut(s) 217
Psp6I CCWGG 1 cut(s) 838
PspCI CACGTG 1 cut(s) 996
PspEI GGTNACC 1 cut(s) 755
PspGI CCWGG 1 cut(s) 838
PspN4I GGNNCC 3 cut(s) 532, 945, 959
PspPI GGNCC 4 cut(s) 158, 217, 552, 836
PspPPI RGGWCCY 1 cut(s) 217
RruI TCGCGA 1 cut(s) 334
RsaI GTAC 3 cut(s) 135, 147, 959
RsaNI GTAC 3 cut(s) 134, 146, 958
RseI CAYNNNNRTG 1 cut(s) 153
SapI GCTCTTC 1 cut(s) 118
SaqAI TTAA 2 cut(s) 27, 591
SatI GCNGC 6 cut(s) 447, 465, 613, 921, 1057, 1083
Sau3AI GATC 2 cut(s) 205, 891
Sau96I GGNCC 4 cut(s) 158, 217, 552, 836
ScaI AGTACT 1 cut(s) 135
SchI GAGTC 1 cut(s) 246
ScrFI CCNGG 3 cut(s) 840, 905, 1087
SduI GDGCHC 1 cut(s) 1111
SfaNI GCATC 8 cut(s) 41, 137, 256, 338, 599, 651, 876, 1193
SinI GGWCC 2 cut(s) 217, 836
SmiMI CAYNNNNRTG 1 cut(s) 153
SmlI CTYRAG 1 cut(s) 831
SmoI CTYRAG 1 cut(s) 831
Sse9I AATT 2 cut(s) 72, 806
SspI AATATT 1 cut(s) 92
SspMI CTAG 1 cut(s) 480
StyD4I CCNGG 3 cut(s) 838, 903, 1085
StyI CCWWGG 1 cut(s) 625
TaaI ACNGT 7 cut(s) 274, 519, 541, 596, 714, 802, 1069
TaiI ACGT 4 cut(s) 184, 876, 998, 1077
TaqI TCGA 5 cut(s) 234, 399, 687, 791, 974
TaqII GACCGA 2 cut(s) 358, 647
TasI AATT 2 cut(s) 72, 806
TatI WGTACW 1 cut(s) 133
TauI GCSGC 4 cut(s) 449, 615, 923, 1059
TfiI GAWTC 1 cut(s) 1014
Tru1I TTAA 2 cut(s) 27, 591
Tru9I TTAA 2 cut(s) 27, 591
TscAI CASTG 1 cut(s) 930
TseI GCWGC 2 cut(s) 464, 1082
TspDTI ATGAA 3 cut(s) 53, 102, 965
TspRI CASTG 1 cut(s) 930
VpaK11BI GGWCC 2 cut(s) 217, 836
XapI RAATTY 1 cut(s) 806
XceI RCATGY 1 cut(s) 12
XspI CTAG 1 cut(s) 480
ZrmI AGTACT 1 cut(s) 135
Zsp2I ATGCAT 1 cut(s) 34
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.