MD08G1050900.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000845
Physical Location & Seq
Reverse (-)
3997534 .. 4001328
3795 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1050900.v1.1.491

Sequence Viewer

Length: 1527 bp
ATGGCAGCAACTCCCGGTATTTCTAGCTCCTCCTCCTCCTCCTCCTCCCTCATGAGCTATTTACTTTACTTCTCTGTAATTCTAACCCTAATTCTCAGCTCTTTGGAGAAGGGCTTTGCCTTGGAAGAGAACCAGCGTCCCCGTACAGTTGAACCCCATCCAGTTTCTCATACAGTTGAACTCAATTCTCTGCTGCCAGCAACCTCATGCAGCCCTAGCTCCTCCGCCAAAGGTGGTGAGAGCAAGGAGTCATCATCTTCATCGGTACTAAAAGTGGTACACAAGCACGGACCATGCTCTCGAATCAACAAGAACAAATCCAAAACTCTAACTCATGCTCAAATCCTCAACCAGGACCAAGCCCGAGTCGACTCAATCCACTCCCTATTCAACACCAATAAATCACCATCCACAACCACGGTCGGTAAACCCCATCCATCCGCCGCCGCCACCATCCCCGCCAAGTCCGGAAGCGTACTGGGCTCGGGCAATTACATTGTCAACGTGGGTCTGGGCTCACCCAAGAAGCAGCTTTCCCTCATTTTCGACACAGGCAGCGATCTAACGTGGACCCAATGCCGCCCATGTGCTGGTTCGTGTTACGACCAGAAGGAGCCCATCTTTGATCCCGCAACCTCCGCCTCATACGTTAATGTCTCATGCAATTCTGCCACATGTTCCCAGCTGGCGTCAGCCACGGGATACAACCCCCGCTGCTACACTTCCACATGCATCTACACCATTGGGTACGGAGACCGGTCCTTCTCCGTCGGATATTTCGGGAAAGAGCGACTGAGCCTGACGTCGACGGATGTTTTTGATGGGTTTTTGTTCGGATGCGGCCAGAACAACCAGGGCCATTTCGGGGGTGCCGCCGGTTTGCTCGGTCTTGGCCGGGATAACATCTCCCTCGTCGAGCAATCCGCCTTGAAATACAACCGGTTCTTCTCCTATTGCCTCCCCTCCACATCTAGCGGCACTGGATACCTCAGATTCGGCAAAGGCGGCCGATCTTCCAAGGGCGTGAAATTCACACCCCTCGTCGACGTCTCCGACAGCGGGTCGTTTTACGGCCTCAACGTGGTCGGGATCAGCGTTGGCGGGAAAAAATTACCGATTTCCGGTGAAGTTTTTTCCTCCTCGGGGACGATTATAGATTCCGGGACGGTGATCACGCGCTTGCAGGCGACGGCGTACAGGGCACTGCGGGACGCGTTTCGGCAGCAGATGAAGAGCTACACCTCGGCTCCGGCGCTTTCTATTCTCGACACTTGCTACGACTTCAGCAATGTTAAAACGCCGACGTATCCGAAGATTTCGTTTGCTTTCGGCGGAGGGATGACGTTGGATTTGGACGAGACGGGGATAATGTATGTGGCGAGTGCCGATCAAGTTTGCCTGGCGTTTGCCGGGAACGGCGACGACAGCGACGTTGGGATATTTGGTAACGTTCAGCAGAAGAGGCTGCAGGTGGTGTATGATGTAGGTGGTCGTAAAATCGGATTTGCCCCTGCAGGATGCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

509

Amino Acids

53.62

Weight (kDa)

8.79

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 164 - 505 2e-23 Eukaryotic aspartyl protease
TAXi_N PF14543 165 - 334 5.4e-52 Xylanase inhibitor N-terminal
TAXi_C PF14541 357 - 503 9.4e-31 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1459
AatII GACGTC 2 cut(s) 806, 1050
Acc36I ACCTGC 1 cut(s) 1459
AccB1I GGYRCC 1 cut(s) 869
AccB7I CCANNNNNTGG 1 cut(s) 590
AccI GTMKAC 3 cut(s) 369, 806, 1044
AccII CGCG 2 cut(s) 1177, 1214
AccIII TCCGGA 1 cut(s) 467
AclI AACGTT 1 cut(s) 1449
AclWI GGATC 2 cut(s) 620, 1097
AcoI YGGCCR 3 cut(s) 841, 892, 1006
AcsI RAATTY 1 cut(s) 1028
AcuI CTGAAG 1 cut(s) 1267
AcyI GRCGYC 3 cut(s) 689, 803, 1047
AfaI GTAC 6 cut(s) 145, 267, 279, 477, 749, 1196
AfiI CCNNNNNNNGG 7 cut(s) 352, 590, 865, 1059, 1081, 1121, 1143
AflIII ACRYGT 2 cut(s) 674, 1212
AgeI ACCGGT 2 cut(s) 756, 939
AgsI TTSAA 4 cut(s) 152, 179, 391, 931
AhdI GACNNNNNGTC 1 cut(s) 1060
AjnI CCWGG 3 cut(s) 351, 852, 1398
AluBI AGCT 7 cut(s) 27, 57, 99, 219, 532, 685, 1236
AluI AGCT 7 cut(s) 27, 57, 99, 219, 532, 685, 1236
Alw26I GTCTC 4 cut(s) 661, 747, 1054, 1352
AlwI GGATC 2 cut(s) 620, 1097
Ama87I CYCGRG 3 cut(s) 363, 484, 1141
Aor13HI TCCGGA 1 cut(s) 467
AoxI GGCC 5 cut(s) 841, 856, 892, 1006, 1072
ApeKI GCWGC 8 cut(s) 5, 193, 210, 529, 555, 714, 1222, 1465
ApoI RAATTY 1 cut(s) 1028
ArsI GACNNNNNNTTYG 2 cut(s) 1333, 1365
AsiGI ACCGGT 2 cut(s) 756, 939
AspLEI GCGC 2 cut(s) 1179, 1255
AspS9I GGNCC 5 cut(s) 290, 355, 570, 759, 856
AsuC2I CCSGG 4 cut(s) 15, 896, 1162, 1411
AsuHPI GGTGA 5 cut(s) 248, 396, 510, 1136, 1180
AvaI CYCGRG 3 cut(s) 363, 484, 1141
AvaII GGWCC 4 cut(s) 290, 355, 570, 759
BaeGI GKGCMC 1 cut(s) 1204
BanI GGYRCC 1 cut(s) 869
BanII GRGCYC 3 cut(s) 485, 518, 618
BbvI GCAGC 8 cut(s) 17, 180, 222, 541, 567, 701, 1234, 1452
BccI CCATC 7 cut(s) 165, 415, 441, 445, 461, 626, 815
BceAI ACGGC 3 cut(s) 1087, 1206, 1432
BciT130I CCWGG 3 cut(s) 353, 854, 1400
BciVI GTATCC 3 cut(s) 695, 977, 1317
BclI TGATCA 1 cut(s) 1170
BcnI CCSGG 4 cut(s) 15, 896, 1162, 1411
BcoDI GTCTC 4 cut(s) 661, 747, 1054, 1352
BfaI CTAG 3 cut(s) 24, 216, 972
BfmI CTRYAG 2 cut(s) 1466, 1512
BfoI RGCGCY 1 cut(s) 1256
BfuAI ACCTGC 1 cut(s) 1459
BfuI GTATCC 3 cut(s) 695, 977, 1317
Bme1390I CCNGG 7 cut(s) 15, 353, 854, 896, 1162, 1400, 1411
Bme18I GGWCC 4 cut(s) 290, 355, 570, 759
BmeRI GACNNNNNGTC 1 cut(s) 1060
BmeT110I CYCGRG 3 cut(s) 363, 484, 1141
BmgT120I GGNCC 5 cut(s) 290, 355, 570, 759, 856
BmiI GGNNCC 4 cut(s) 572, 615, 871, 1248
BmrFI CCNGG 7 cut(s) 15, 353, 854, 896, 1162, 1400, 1411
BmrI ACTGGG 1 cut(s) 488
BmsI GCATC 3 cut(s) 741, 827, 1508
BmuI ACTGGG 1 cut(s) 488
BpuMI CCSGG 4 cut(s) 15, 896, 1162, 1411
BsaHI GRCGYC 3 cut(s) 689, 803, 1047
BsaI GGTCTC 1 cut(s) 747
BsaJI CCNNGG 7 cut(s) 120, 417, 696, 853, 1017, 1140, 1242
BsaWI WCCGGW 4 cut(s) 467, 756, 939, 1121
BsaXI ACNNNNNCTCC 2 cut(s) 1231, 1261
Bsc4I CCNNNNNNNGG 7 cut(s) 352, 590, 865, 1059, 1081, 1121, 1143
Bse118I RCCGGY 3 cut(s) 756, 875, 939
Bse1I ACTGG 3 cut(s) 161, 483, 985
Bse3DI GCAATG 1 cut(s) 1294
BseAI TCCGGA 1 cut(s) 467
BseBI CCWGG 3 cut(s) 353, 854, 1400
BseDI CCNNGG 7 cut(s) 120, 417, 696, 853, 1017, 1140, 1242
BseGI GGATG 9 cut(s) 157, 407, 433, 437, 453, 817, 842, 1344, 1523
BseLI CCNNNNNNNGG 7 cut(s) 352, 590, 865, 1059, 1081, 1121, 1143
BseMI GCAATG 1 cut(s) 1294
BseMII CTCAG 3 cut(s) 109, 785, 1003
BseNI ACTGG 3 cut(s) 161, 483, 985
BseRI GAGGAG 8 cut(s) 19, 22, 25, 28, 31, 34, 211, 1129
BseSI GKGCMC 1 cut(s) 1204
BseX3I CGGCCG 1 cut(s) 1006
BseXI GCAGC 8 cut(s) 17, 180, 222, 541, 567, 701, 1234, 1452
BseYI CCCAGC 1 cut(s) 681
Bsh1236I CGCG 2 cut(s) 1177, 1214
Bsh1285I CGRYCG 2 cut(s) 423, 1009
BshFI GGCC 5 cut(s) 843, 858, 894, 1008, 1074
BshNI GGYRCC 1 cut(s) 869
BshTI ACCGGT 2 cut(s) 756, 939
BsiEI CGRYCG 2 cut(s) 423, 1009
BsiHKCI CYCGRG 3 cut(s) 363, 484, 1141
BslFI GGGAC 4 cut(s) 123, 1159, 1177, 1223
BslI CCNNNNNNNGG 7 cut(s) 352, 590, 865, 1059, 1081, 1121, 1143
BsmAI GTCTC 4 cut(s) 661, 747, 1054, 1352
BsmBI CGTCTC 2 cut(s) 1054, 1352
BsmFI GGGAC 4 cut(s) 123, 1159, 1177, 1223
BsnI GGCC 5 cut(s) 843, 858, 894, 1008, 1074
Bso31I GGTCTC 1 cut(s) 747
BsoBI CYCGRG 3 cut(s) 363, 484, 1141
Bsp1286I GDGCHC 4 cut(s) 485, 518, 618, 1204
Bsp13I TCCGGA 1 cut(s) 467
Bsp143I GATC 6 cut(s) 559, 625, 1010, 1089, 1170, 1387
BspANI GGCC 5 cut(s) 843, 858, 894, 1008, 1074
BspCNI CTCAG 3 cut(s) 108, 786, 1002
BspEI TCCGGA 1 cut(s) 467
BspFNI CGCG 2 cut(s) 1177, 1214
BspHI TCATGA 1 cut(s) 51
BspLI GGNNCC 4 cut(s) 572, 615, 871, 1248
BspMAI CTGCAG 2 cut(s) 1470, 1516
BspMI ACCTGC 1 cut(s) 1459
BspPI GGATC 2 cut(s) 620, 1097
BspQI GCTCTTC 1 cut(s) 1226
BspT107I GGYRCC 1 cut(s) 869
BspTNI GGTCTC 1 cut(s) 747
BsrDI GCAATG 1 cut(s) 1294
BsrFI RCCGGY 3 cut(s) 756, 875, 939
BsrI ACTGG 3 cut(s) 161, 483, 985
BssAI RCCGGY 3 cut(s) 756, 875, 939
BssECI CCNNGG 7 cut(s) 120, 417, 696, 853, 1017, 1140, 1242
BssMI GATC 6 cut(s) 559, 625, 1010, 1089, 1170, 1387
BssNI GRCGYC 3 cut(s) 689, 803, 1047
BssT1I CCWWGG 2 cut(s) 120, 1017
Bst2UI CCWGG 3 cut(s) 353, 854, 1400
Bst4CI ACNGT 4 cut(s) 148, 175, 421, 1168
Bst6I CTCTTC 3 cut(s) 120, 1226, 1454
BstACI GRCGYC 3 cut(s) 689, 803, 1047
BstC8I GCNNGC 4 cut(s) 198, 687, 1181, 1185
BstDEI CTNAG 3 cut(s) 95, 794, 989
BstDSI CCRYGG 2 cut(s) 417, 696
BstENI CCTNNNNNAGG 1 cut(s) 350
BstF5I GGATG 9 cut(s) 157, 407, 433, 437, 453, 817, 842, 1344, 1523
BstFNI CGCG 2 cut(s) 1177, 1214
BstH2I RGCGCY 1 cut(s) 1256
BstHHI GCGC 2 cut(s) 1179, 1255
BstKTI GATC 6 cut(s) 562, 628, 1013, 1092, 1173, 1390
BstMAI GTCTC 4 cut(s) 661, 747, 1054, 1352
BstMBI GATC 6 cut(s) 559, 625, 1010, 1089, 1170, 1387
BstMCI CGRYCG 2 cut(s) 423, 1009
BstMWI GCNNNNNNNGC 7 cut(s) 216, 480, 638, 1005, 1199, 1425, 1462
BstNI CCWGG 3 cut(s) 353, 854, 1400
BstNSI RCATGY 2 cut(s) 678, 732
BstSCI CCNGG 7 cut(s) 13, 351, 852, 894, 1160, 1398, 1409
BstSFI CTRYAG 2 cut(s) 1466, 1512
BstSLI GKGCMC 1 cut(s) 1204
BstUI CGCG 2 cut(s) 1177, 1214
BstV1I GCAGC 8 cut(s) 17, 180, 222, 541, 567, 701, 1234, 1452
BstZI CGGCCG 1 cut(s) 1006
BsuI GTATCC 3 cut(s) 695, 977, 1317
BsuRI GGCC 5 cut(s) 843, 858, 894, 1008, 1074
BtgI CCRYGG 2 cut(s) 417, 696
BtsCI GGATG 9 cut(s) 157, 407, 433, 437, 453, 817, 842, 1344, 1523
BtsI GCAGTG 1 cut(s) 1202
BtsIMutI CAGTG 2 cut(s) 978, 1202
BveI ACCTGC 1 cut(s) 1459
Cac8I GCNNGC 4 cut(s) 198, 687, 1181, 1185
CciI TCATGA 1 cut(s) 51
CfoI GCGC 2 cut(s) 1179, 1255
Cfr10I RCCGGY 3 cut(s) 756, 875, 939
Cfr13I GGNCC 5 cut(s) 290, 355, 570, 759, 856
CseI GACGC 3 cut(s) 125, 678, 1220
Csp6I GTAC 6 cut(s) 144, 266, 278, 476, 748, 1195
CspAI ACCGGT 2 cut(s) 756, 939
CviAII CATG 8 cut(s) 52, 207, 294, 335, 585, 660, 675, 729
CviQI GTAC 6 cut(s) 144, 266, 278, 476, 748, 1195
DdeI CTNAG 3 cut(s) 95, 794, 989
DpnI GATC 6 cut(s) 561, 627, 1012, 1091, 1172, 1389
DpnII GATC 6 cut(s) 559, 625, 1010, 1089, 1170, 1387
DriI GACNNNNNGTC 1 cut(s) 1060
EaeI YGGCCR 3 cut(s) 841, 892, 1006
EagI CGGCCG 1 cut(s) 1006
Eam1104I CTCTTC 3 cut(s) 120, 1226, 1454
Eam1105I GACNNNNNGTC 1 cut(s) 1060
EarI CTCTTC 3 cut(s) 120, 1226, 1454
EciI GGCGGA 5 cut(s) 214, 430, 628, 913, 1347
EclXI CGGCCG 1 cut(s) 1006
Eco130I CCWWGG 2 cut(s) 120, 1017
Eco24I GRGCYC 3 cut(s) 485, 518, 618
Eco31I GGTCTC 1 cut(s) 747
Eco47I GGWCC 4 cut(s) 290, 355, 570, 759
Eco52I CGGCCG 1 cut(s) 1006
Eco57I CTGAAG 1 cut(s) 1267
Eco88I CYCGRG 3 cut(s) 363, 484, 1141
EcoNI CCTNNNNNAGG 1 cut(s) 350
EcoRII CCWGG 3 cut(s) 351, 852, 1398
EcoT14I CCWWGG 2 cut(s) 120, 1017
EcoT22I ATGCAT 1 cut(s) 734
EcoT38I GRGCYC 3 cut(s) 485, 518, 618
ErhI CCWWGG 2 cut(s) 120, 1017
Esp3I CGTCTC 2 cut(s) 1054, 1352
FaeI CATG 8 cut(s) 55, 210, 297, 338, 588, 663, 678, 732
FaqI GGGAC 4 cut(s) 123, 1159, 1177, 1223
FatI CATG 8 cut(s) 51, 206, 293, 334, 584, 659, 674, 728
FauI CCCGC 6 cut(s) 466, 637, 719, 1052, 1094, 1200
FbaI TGATCA 1 cut(s) 1170
FblI GTMKAC 3 cut(s) 369, 806, 1044
FokI GGATG 8 cut(s) 144, 394, 420, 424, 440, 824, 849, 1351
FriOI GRGCYC 3 cut(s) 485, 518, 618
FspBI CTAG 3 cut(s) 24, 216, 972
GlaI GCGC 2 cut(s) 1178, 1254
GsaI CCCAGC 1 cut(s) 685
HaeII RGCGCY 1 cut(s) 1256
HaeIII GGCC 5 cut(s) 843, 858, 894, 1008, 1074
HgaI GACGC 3 cut(s) 125, 678, 1220
HhaI GCGC 2 cut(s) 1179, 1255
Hin1I GRCGYC 3 cut(s) 689, 803, 1047
Hin1II CATG 8 cut(s) 55, 210, 297, 338, 588, 663, 678, 732
Hin6I GCGC 2 cut(s) 1177, 1253
HinP1I GCGC 2 cut(s) 1177, 1253
HincII GTYRAC 4 cut(s) 370, 502, 807, 1045
HindII GTYRAC 4 cut(s) 370, 502, 807, 1045
HinfI GANTC 6 cut(s) 248, 303, 366, 371, 993, 1157
HphI GGTGA 5 cut(s) 248, 396, 510, 1136, 1180
Hpy166II GTNNAC 7 cut(s) 280, 370, 428, 502, 570, 807, 1045
Hpy188I TCNGA 6 cut(s) 773, 836, 992, 1054, 1311, 1502
Hpy188III TCNNGA 6 cut(s) 52, 300, 468, 781, 1087, 1265
Hpy8I GTNNAC 7 cut(s) 280, 370, 428, 502, 570, 807, 1045
HpyAV CCTTC 3 cut(s) 103, 604, 772
HpyCH4III ACNGT 4 cut(s) 148, 175, 421, 1168
HpyCH4V TGCA 6 cut(s) 210, 663, 732, 1183, 1468, 1514
HpyF10VI GCNNNNNNNGC 7 cut(s) 216, 480, 638, 1005, 1199, 1425, 1462
HpyF3I CTNAG 3 cut(s) 95, 794, 989
Hsp92I GRCGYC 3 cut(s) 689, 803, 1047
Hsp92II CATG 8 cut(s) 55, 210, 297, 338, 588, 663, 678, 732
HspAI GCGC 2 cut(s) 1177, 1253
Kpn2I TCCGGA 1 cut(s) 467
Ksp22I TGATCA 1 cut(s) 1170
Kzo9I GATC 6 cut(s) 559, 625, 1010, 1089, 1170, 1387
LguI GCTCTTC 1 cut(s) 1226
LmnI GCTCC 4 cut(s) 32, 224, 613, 1252
Lsp1109I GCAGC 8 cut(s) 17, 180, 222, 541, 567, 701, 1234, 1452
LweI GCATC 3 cut(s) 741, 827, 1508
MaeI CTAG 3 cut(s) 24, 216, 972
MaeIII GTNAC 2 cut(s) 599, 1445
MalI GATC 6 cut(s) 561, 627, 1012, 1091, 1172, 1389
MboI GATC 6 cut(s) 559, 625, 1010, 1089, 1170, 1387
MboII GAAGA 7 cut(s) 137, 249, 937, 1005, 1243, 1324, 1471
MhlI GDGCHC 4 cut(s) 485, 518, 618, 1204
MluCI AATT 7 cut(s) 78, 90, 184, 490, 664, 1028, 1109
MluI ACGCGT 1 cut(s) 1212
MlyI GAGTC 3 cut(s) 257, 365, 375
MmeI TCCRAC 3 cut(s) 751, 1077, 1326
Mph1103I ATGCAT 1 cut(s) 734
MroI TCCGGA 1 cut(s) 467
MseI TTAA 2 cut(s) 651, 1293
MspA1I CMGCKG 3 cut(s) 685, 714, 1059
MspR9I CCNGG 7 cut(s) 15, 353, 854, 896, 1162, 1400, 1411
MvaI CCWGG 3 cut(s) 353, 854, 1400
MvnI CGCG 2 cut(s) 1177, 1214
MwoI GCNNNNNNNGC 7 cut(s) 216, 480, 638, 1005, 1199, 1425, 1462
NciI CCSGG 4 cut(s) 15, 896, 1162, 1411
NdeII GATC 6 cut(s) 559, 625, 1010, 1089, 1170, 1387
NlaIII CATG 8 cut(s) 55, 210, 297, 338, 588, 663, 678, 732
NlaIV GGNNCC 4 cut(s) 572, 615, 871, 1248
NmeAIII GCCGAG 1 cut(s) 1223
NsiI ATGCAT 1 cut(s) 734
NspI RCATGY 2 cut(s) 678, 732
PagI TCATGA 1 cut(s) 51
PaqCI CACCTGC 1 cut(s) 1459
PciI ACATGT 1 cut(s) 674
PciSI GCTCTTC 1 cut(s) 1226
PcsI WCGNNNNNNNCGW 4 cut(s) 1050, 1077, 1092, 1428
PfeI GAWTC 3 cut(s) 303, 993, 1157
PflMI CCANNNNNTGG 1 cut(s) 590
PfoI TCCNGGA 1 cut(s) 1160
PinAI ACCGGT 2 cut(s) 756, 939
PleI GAGTC 3 cut(s) 256, 365, 374
PpsI GAGTC 3 cut(s) 256, 365, 374
PscI ACATGT 1 cut(s) 674
Psp1406I AACGTT 1 cut(s) 1449
Psp6I CCWGG 3 cut(s) 351, 852, 1398
PspFI CCCAGC 1 cut(s) 681
PspGI CCWGG 3 cut(s) 351, 852, 1398
PspN4I GGNNCC 4 cut(s) 572, 615, 871, 1248
PspPI GGNCC 5 cut(s) 290, 355, 570, 759, 856
PstI CTGCAG 2 cut(s) 1470, 1516
PvuII CAGCTG 1 cut(s) 685
RsaI GTAC 6 cut(s) 145, 267, 279, 477, 749, 1196
RsaNI GTAC 6 cut(s) 144, 266, 278, 476, 748, 1195
SalI GTCGAC 3 cut(s) 368, 805, 1043
SapI GCTCTTC 1 cut(s) 1226
SaqAI TTAA 2 cut(s) 651, 1293
Sau3AI GATC 6 cut(s) 559, 625, 1010, 1089, 1170, 1387
Sau96I GGNCC 5 cut(s) 290, 355, 570, 759, 856
SbfI CCTGCAGG 1 cut(s) 1516
SchI GAGTC 3 cut(s) 257, 365, 375
ScrFI CCNGG 7 cut(s) 15, 353, 854, 896, 1162, 1400, 1411
SdaI CCTGCAGG 1 cut(s) 1516
SduI GDGCHC 4 cut(s) 485, 518, 618, 1204
SfaNI GCATC 3 cut(s) 741, 827, 1508
SfcI CTRYAG 2 cut(s) 1466, 1512
SgrDI CGTCGACG 2 cut(s) 805, 1043
SinI GGWCC 4 cut(s) 290, 355, 570, 759
Sse8387I CCTGCAGG 1 cut(s) 1516
Sse9I AATT 7 cut(s) 78, 90, 184, 490, 664, 1028, 1109
SspMI CTAG 3 cut(s) 24, 216, 972
StyD4I CCNGG 7 cut(s) 13, 351, 852, 894, 1160, 1398, 1409
StyI CCWWGG 2 cut(s) 120, 1017
TaaI ACNGT 4 cut(s) 148, 175, 421, 1168
TaqI TCGA 7 cut(s) 301, 369, 546, 806, 915, 1044, 1266
TaqII GACCGA 1 cut(s) 875
TasI AATT 7 cut(s) 78, 90, 184, 490, 664, 1028, 1109
TauI GCSGC 7 cut(s) 446, 449, 582, 843, 875, 978, 1008
TfiI GAWTC 3 cut(s) 303, 993, 1157
Tru1I TTAA 2 cut(s) 651, 1293
Tru9I TTAA 2 cut(s) 651, 1293
TscAI CASTG 2 cut(s) 985, 1209
TseI GCWGC 8 cut(s) 5, 193, 210, 529, 555, 714, 1222, 1465
TspDTI ATGAA 2 cut(s) 249, 1244
TspGWI ACGGA 4 cut(s) 303, 757, 765, 824
TspRI CASTG 2 cut(s) 985, 1209
Van91I CCANNNNNTGG 1 cut(s) 590
VpaK11BI GGWCC 4 cut(s) 290, 355, 570, 759
XagI CCTNNNNNAGG 1 cut(s) 350
XapI RAATTY 1 cut(s) 1028
XceI RCATGY 2 cut(s) 678, 732
XmiI GTMKAC 3 cut(s) 369, 806, 1044
XspI CTAG 3 cut(s) 24, 216, 972
ZraI GACGTC 2 cut(s) 804, 1048
Zsp2I ATGCAT 1 cut(s) 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.