Rw1G023920

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000845
Physical Location & Seq
Forward (+)
49942197 .. 49944947
2751 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G023920.1

Sequence Viewer

Length: 1467 bp
ATGGCAACTCCCAGTGTTCATTCTGGCTGCTCCTTCCTCGCCAACAAGTACTTGATTTCCTTTTGTGTATTTGTATGCCTTAATATTCTCTCTTCTTTGGACAAGGGCTTCGCTTTGAGTACTCAGACAGATGTTGAACTCAGCTCTCTACTACCAGCATCGACCTGCAGCCCCTCCACCAGAGGTCATGATAAGAAGAAGGCAACACTTAAAGTGGTACACAGGCATGGTCCATGCTCTAAGATGTCCCAGCCTAACGAATACAAGACCACAACTCCAACTCACACTCAGATCCTCCAGCAGGACCAAGCCCGAGTCACCTCAATCCACTCCCGGATGGGTGACAACGTAAGGCAAACCGACGCATCCATCCCCGCCAAGTCCGGCAGCGTAGTGGGCTCGGGAAACTACATTGTGACCGTGGGCTTGGGCTCACCCGCAAAACAACTCTCGCTCATATTTGACACCGGGAGCGACCTCACTTGGACTCAGTGTCAACCTTGTGTCAGGTCATGTTACAAACAGAAGGAGCCCATCTTTGACCCATCCCTCTCCAAATCGTATACCAACATAACCTGTAATTCGCCATTCTGCTCTCAACTCATATCAGCCACAGGTACCAGACCTTATTGCACAAACAGCACATCAACTTGCGTATACAACATACAATACAGAGATAACTCCTTATCCGTGGGATTCTTTGCTAAAGAAAGGTTGACACTAACGTCAACGGACTATTTCGATGGGTTTCTCTTCGGCTGTGGCCAAGGCCAAAACAATCAAGGCCTTTTTGGCGGGTCTGCCGGGTTATTGGGCCTGGGCCCTAACAAAATCTCCCTTGTCGAACAGAGTGCCCAAAAGTACGGCCGCTTCTTCTCCTACTGCCTACCCTCAACTTCTTGCGCCACCGGTTACCTCAGCTTTGGAAAAGGCGGCGAACCCTCCAAAGACGTCAAGTTCATGCCGCTCTCCATTGTATCTGGACGCGGGTACTTAGGAGGGTCCTTTTACGGCCTGGATGTCGTTGGAATCAACGTCGGCGGACGTCGGGTATCGATTCCTGCTTCGGTTTTTTCGTCCTCAGGGACCATCATTGACACAGGGACGATGATAACGCGACTTCCGGCAACGGCGTACAATGCTTTGAGGGATGAGTTTAAGCGAGGAATGAAAAGCTACCCGCAAGCTGAGGCGCTTTCGATACTGGACACGTGCTACGACCTGAGCGGGAGCAGCAAGGTGTCGTACCCCAAAATAGCGTTCGCTTTCAGCGGTGGATTGACATTGGATTTGGATGCGACAGGCATATTTTACGTGGCCAATGCTTCACAGGTGTGCTTGGCGTTCGCTGGGAATAGTGATGACAGCGACATTGCCATATTTGGGAATGTTCAACAGAAACGGTTGCAGGTAGTGTATGACGTCGCCGGTGGAAAGATTGGATTTGCCCCAGCTGGTTGCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

488

Amino Acids

52.03

Weight (kDa)

8.76

Isoelectric Point (pI)

37.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 136 - 190 1.8e-07 Eukaryotic aspartyl protease
TAXi_N PF14543 137 - 310 7.3e-53 Xylanase inhibitor N-terminal
Asp PF00026 217 - 485 4.3e-13 Eukaryotic aspartyl protease
TAXi_C PF14541 337 - 483 2.9e-29 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 724
AatII GACGTC 3 cut(s) 954, 1048, 1425
Acc36I ACCTGC 2 cut(s) 173, 1399
Acc65I GGTACC 1 cut(s) 617
AccB1I GGYRCC 1 cut(s) 617
AccBSI CCGCTC 2 cut(s) 967, 1227
AccI GTMKAC 2 cut(s) 563, 657
AccII CGCG 2 cut(s) 987, 1117
AclWI GGATC 1 cut(s) 286
AcoI YGGCCR 3 cut(s) 763, 865, 1317
AcvI CACGTG 1 cut(s) 1212
AcyI GRCGYC 3 cut(s) 951, 1045, 1422
AfaI GTAC 8 cut(s) 50, 121, 219, 619, 863, 992, 1136, 1247
AfiI CCNNNNNNNGG 2 cut(s) 301, 1383
AflIII ACRYGT 1 cut(s) 1209
AgeI ACCGGT 1 cut(s) 908
AgsI TTSAA 2 cut(s) 137, 1394
AhdI GACNNNNNGTC 1 cut(s) 492
AjnI CCWGG 2 cut(s) 816, 1014
AjuI GAANNNNNNNTTGG 2 cut(s) 1244, 1276
AleI CACNNNNGTG 1 cut(s) 1333
AluBI AGCT 5 cut(s) 144, 921, 1176, 1187, 1454
AluI AGCT 5 cut(s) 144, 921, 1176, 1187, 1454
AlwI GGATC 1 cut(s) 286
Ama87I CYCGRG 2 cut(s) 312, 400
AoxI GGCC 8 cut(s) 763, 769, 784, 814, 820, 865, 1012, 1317
ApaI GGGCCC 1 cut(s) 824
ApeKI GCWGC 4 cut(s) 27, 168, 387, 1233
ArsI GACNNNNNNTTYG 4 cut(s) 92, 124, 1273, 1305
AsiGI ACCGGT 1 cut(s) 908
Asp718I GGTACC 1 cut(s) 617
AspLEI GCGC 2 cut(s) 905, 1195
AspS9I GGNCC 7 cut(s) 230, 304, 814, 820, 821, 1002, 1086
AsuC2I CCSGG 3 cut(s) 334, 469, 805
AsuHPI GGTGA 3 cut(s) 310, 353, 426
AvaI CYCGRG 2 cut(s) 312, 400
AvaII GGWCC 4 cut(s) 230, 304, 1002, 1086
AxyI CCTNAGG 1 cut(s) 1081
BaeGI GKGCMC 2 cut(s) 824, 856
BalI TGGCCA 2 cut(s) 765, 1319
BanI GGYRCC 1 cut(s) 617
BanII GRGCYC 4 cut(s) 401, 434, 534, 824
BarI GAAGNNNNNNTAC 2 cut(s) 854, 886
BbrPI CACGTG 1 cut(s) 1212
BbvCI CCTCAGC 2 cut(s) 917, 1188
BbvI GCAGC 4 cut(s) 14, 180, 399, 1245
BccI CCATC 6 cut(s) 331, 377, 542, 553, 737, 1097
BceAI ACGGC 3 cut(s) 880, 1027, 1146
BcgI CGANNNNNNTGC 2 cut(s) 833, 867
BciT130I CCWGG 2 cut(s) 818, 1016
BcnI CCSGG 3 cut(s) 334, 469, 805
BfmI CTRYAG 1 cut(s) 166
BfoI RGCGCY 1 cut(s) 1196
BfuAI ACCTGC 2 cut(s) 173, 1399
BglI GCCNNNNNGGC 1 cut(s) 792
BisI GCNGC 7 cut(s) 28, 169, 388, 868, 934, 965, 1234
BlsI GCNGC 7 cut(s) 29, 170, 389, 869, 935, 966, 1235
BmcAI AGTACT 2 cut(s) 50, 121
Bme1390I CCNGG 5 cut(s) 334, 469, 805, 818, 1016
Bme18I GGWCC 4 cut(s) 230, 304, 1002, 1086
BmeRI GACNNNNNGTC 1 cut(s) 492
BmeT110I CYCGRG 2 cut(s) 312, 400
BmgT120I GGNCC 7 cut(s) 230, 304, 814, 820, 821, 1002, 1086
BmiI GGNNCC 5 cut(s) 531, 619, 822, 1003, 1087
BmrFI CCNGG 5 cut(s) 334, 469, 805, 818, 1016
BmrI ACTGGG 1 cut(s) 6
BmsI GCATC 3 cut(s) 167, 374, 1285
BmuI ACTGGG 1 cut(s) 6
BpmI CTGGAG 1 cut(s) 281
Bpu10I CCTNAGC 3 cut(s) 917, 1188, 1223
BpuMI CCSGG 3 cut(s) 334, 469, 805
Bsa29I ATCGAT 1 cut(s) 1055
BsaAI YACGTR 2 cut(s) 1212, 1315
BsaHI GRCGYC 3 cut(s) 951, 1045, 1422
BsaJI CCNNGG 4 cut(s) 420, 690, 766, 817
BsaWI WCCGGW 1 cut(s) 908
BsaXI ACNNNNNCTCC 4 cut(s) 259, 289, 818, 848
Bsc4I CCNNNNNNNGG 2 cut(s) 301, 1383
Bse118I RCCGGY 2 cut(s) 908, 1427
Bse1I ACTGG 2 cut(s) 12, 1209
Bse21I CCTNAGG 1 cut(s) 1081
Bse3DI GCAATG 1 cut(s) 1371
BseBI CCWGG 2 cut(s) 818, 1016
BseCI ATCGAT 1 cut(s) 1055
BseDI CCNNGG 4 cut(s) 420, 690, 766, 817
BseGI GGATG 7 cut(s) 342, 365, 369, 545, 1024, 1156, 1300
BseLI CCNNNNNNNGG 2 cut(s) 301, 1383
BseMI GCAATG 1 cut(s) 1371
BseMII CTCAG 8 cut(s) 137, 154, 302, 503, 931, 1095, 1179, 1214
BseNI ACTGG 2 cut(s) 12, 1209
BseSI GKGCMC 2 cut(s) 824, 856
BseX3I CGGCCG 1 cut(s) 865
BseXI GCAGC 4 cut(s) 14, 180, 399, 1245
BseYI CCCAGC 3 cut(s) 249, 1349, 1450
Bsh1236I CGCG 2 cut(s) 987, 1117
Bsh1285I CGRYCG 1 cut(s) 868
BshFI GGCC 8 cut(s) 765, 771, 786, 816, 822, 867, 1014, 1319
BshNI GGYRCC 1 cut(s) 617
BshTI ACCGGT 1 cut(s) 908
BshVI ATCGAT 1 cut(s) 1055
BsiEI CGRYCG 1 cut(s) 868
BsiHKCI CYCGRG 2 cut(s) 312, 400
BsiSI CCGG 7 cut(s) 334, 384, 468, 804, 909, 1124, 1428
BslFI GGGAC 3 cut(s) 232, 1099, 1117
BslI CCNNNNNNNGG 2 cut(s) 301, 1383
BsmFI GGGAC 3 cut(s) 232, 1099, 1117
BsnI GGCC 8 cut(s) 765, 771, 786, 816, 822, 867, 1014, 1319
BsoBI CYCGRG 2 cut(s) 312, 400
Bsp120I GGGCCC 1 cut(s) 820
Bsp1286I GDGCHC 5 cut(s) 401, 434, 534, 824, 856
Bsp143I GATC 1 cut(s) 291
BspANI GGCC 8 cut(s) 765, 771, 786, 816, 822, 867, 1014, 1319
BspCNI CTCAG 8 cut(s) 136, 153, 301, 502, 930, 1094, 1180, 1215
BspDI ATCGAT 1 cut(s) 1055
BspFNI CGCG 2 cut(s) 987, 1117
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 5 cut(s) 531, 619, 822, 1003, 1087
BspMAI CTGCAG 1 cut(s) 170
BspMI ACCTGC 2 cut(s) 173, 1399
BspPI GGATC 1 cut(s) 286
BspT107I GGYRCC 1 cut(s) 617
BsrBI CCGCTC 2 cut(s) 967, 1227
BsrDI GCAATG 1 cut(s) 1371
BsrFI RCCGGY 2 cut(s) 908, 1427
BsrI ACTGG 2 cut(s) 12, 1209
BssAI RCCGGY 2 cut(s) 908, 1427
BssECI CCNNGG 4 cut(s) 420, 690, 766, 817
BssMI GATC 1 cut(s) 291
BssNAI GTATAC 2 cut(s) 564, 658
BssNI GRCGYC 3 cut(s) 951, 1045, 1422
BssT1I CCWWGG 1 cut(s) 766
Bst1107I GTATAC 2 cut(s) 564, 658
Bst2UI CCWGG 2 cut(s) 818, 1016
Bst4CI ACNGT 2 cut(s) 421, 1404
Bst6I CTCTTC 2 cut(s) 97, 758
BstACI GRCGYC 3 cut(s) 951, 1045, 1422
BstBAI YACGTR 2 cut(s) 1212, 1315
BstC8I GCNNGC 1 cut(s) 1185
BstDSI CCRYGG 2 cut(s) 420, 690
BstEII GGTNACC 1 cut(s) 911
BstENI CCTNNNNNAGG 1 cut(s) 299
BstF5I GGATG 7 cut(s) 342, 365, 369, 545, 1024, 1156, 1300
BstFNI CGCG 2 cut(s) 987, 1117
BstH2I RGCGCY 1 cut(s) 1196
BstHHI GCGC 2 cut(s) 905, 1195
BstKTI GATC 1 cut(s) 294
BstMBI GATC 1 cut(s) 291
BstMCI CGRYCG 1 cut(s) 868
BstMWI GCNNNNNNNGC 5 cut(s) 396, 639, 792, 1139, 1233
BstNI CCWGG 2 cut(s) 818, 1016
BstPI GGTNACC 1 cut(s) 911
BstSCI CCNGG 5 cut(s) 332, 467, 803, 816, 1014
BstSFI CTRYAG 1 cut(s) 166
BstSLI GKGCMC 2 cut(s) 824, 856
BstUI CGCG 2 cut(s) 987, 1117
BstV1I GCAGC 4 cut(s) 14, 180, 399, 1245
BstX2I RGATCY 1 cut(s) 291
BstYI RGATCY 1 cut(s) 291
BstZ17I GTATAC 2 cut(s) 564, 658
BstZI CGGCCG 1 cut(s) 865
Bsu15I ATCGAT 1 cut(s) 1055
Bsu36I CCTNAGG 1 cut(s) 1081
BsuRI GGCC 8 cut(s) 765, 771, 786, 816, 822, 867, 1014, 1319
BsuTUI ATCGAT 1 cut(s) 1055
BtgI CCRYGG 2 cut(s) 420, 690
BtsCI GGATG 7 cut(s) 342, 365, 369, 545, 1024, 1156, 1300
BtsIMutI CAGTG 2 cut(s) 19, 497
BveI ACCTGC 2 cut(s) 173, 1399
Cac8I GCNNGC 1 cut(s) 1185
CciI TCATGA 1 cut(s) 187
CfoI GCGC 2 cut(s) 905, 1195
Cfr10I RCCGGY 2 cut(s) 908, 1427
Cfr13I GGNCC 7 cut(s) 230, 304, 814, 820, 821, 1002, 1086
ClaI ATCGAT 1 cut(s) 1055
CseI GACGC 2 cut(s) 371, 993
Csp6I GTAC 8 cut(s) 49, 120, 218, 618, 862, 991, 1135, 1246
CspAI ACCGGT 1 cut(s) 908
CviAII CATG 5 cut(s) 188, 227, 234, 513, 961
CviQI GTAC 8 cut(s) 49, 120, 218, 618, 862, 991, 1135, 1246
DpnI GATC 1 cut(s) 293
DpnII GATC 1 cut(s) 291
DrdI GACNNNNNNGTC 1 cut(s) 724
DriI GACNNNNNGTC 1 cut(s) 492
DseDI GACNNNNNNGTC 1 cut(s) 724
EaeI YGGCCR 3 cut(s) 763, 865, 1317
EagI CGGCCG 1 cut(s) 865
Eam1104I CTCTTC 2 cut(s) 97, 758
Eam1105I GACNNNNNGTC 1 cut(s) 492
EarI CTCTTC 2 cut(s) 97, 758
EciI GGCGGA 1 cut(s) 1056
EclXI CGGCCG 1 cut(s) 865
Eco130I CCWWGG 1 cut(s) 766
Eco147I AGGCCT 1 cut(s) 786
Eco24I GRGCYC 4 cut(s) 401, 434, 534, 824
Eco47I GGWCC 4 cut(s) 230, 304, 1002, 1086
Eco52I CGGCCG 1 cut(s) 865
Eco72I CACGTG 1 cut(s) 1212
Eco81I CCTNAGG 1 cut(s) 1081
Eco88I CYCGRG 2 cut(s) 312, 400
Eco91I GGTNACC 1 cut(s) 911
EcoNI CCTNNNNNAGG 1 cut(s) 299
EcoO109I RGGNCCY 2 cut(s) 821, 1002
EcoO65I GGTNACC 1 cut(s) 911
EcoRII CCWGG 2 cut(s) 816, 1014
EcoT14I CCWWGG 1 cut(s) 766
EcoT38I GRGCYC 4 cut(s) 401, 434, 534, 824
ErhI CCWWGG 1 cut(s) 766
FaeI CATG 5 cut(s) 191, 230, 237, 516, 964
FaqI GGGAC 3 cut(s) 232, 1099, 1117
FatI CATG 5 cut(s) 187, 226, 233, 512, 960
FauI CCCGC 6 cut(s) 382, 445, 788, 980, 1188, 1220
FblI GTMKAC 2 cut(s) 563, 657
Fnu4HI GCNGC 7 cut(s) 28, 169, 388, 868, 934, 965, 1234
FokI GGATG 7 cut(s) 349, 352, 356, 532, 1031, 1163, 1307
FriOI GRGCYC 4 cut(s) 401, 434, 534, 824
Fsp4HI GCNGC 7 cut(s) 28, 169, 388, 868, 934, 965, 1234
GlaI GCGC 2 cut(s) 904, 1194
GluI GCNGC 7 cut(s) 28, 169, 388, 868, 934, 965, 1234
GsaI CCCAGC 3 cut(s) 253, 1353, 1454
GsuI CTGGAG 1 cut(s) 281
HaeII RGCGCY 1 cut(s) 1196
HaeIII GGCC 8 cut(s) 765, 771, 786, 816, 822, 867, 1014, 1319
HapII CCGG 7 cut(s) 334, 384, 468, 804, 909, 1124, 1428
HgaI GACGC 2 cut(s) 371, 993
HhaI GCGC 2 cut(s) 905, 1195
Hin1I GRCGYC 3 cut(s) 951, 1045, 1422
Hin1II CATG 5 cut(s) 191, 230, 237, 516, 964
Hin6I GCGC 2 cut(s) 903, 1193
HinP1I GCGC 2 cut(s) 903, 1193
HincII GTYRAC 3 cut(s) 497, 717, 729
HindII GTYRAC 3 cut(s) 497, 717, 729
HinfI GANTC 5 cut(s) 315, 487, 696, 1029, 1057
HpaII CCGG 7 cut(s) 334, 384, 468, 804, 909, 1124, 1428
HphI GGTGA 3 cut(s) 310, 353, 426
Hpy166II GTNNAC 6 cut(s) 220, 497, 564, 658, 717, 729
Hpy188I TCNGA 2 cut(s) 126, 291
Hpy188III TCNNGA 3 cut(s) 188, 402, 981
Hpy8I GTNNAC 6 cut(s) 220, 497, 564, 658, 717, 729
Hpy99I CGWCG 4 cut(s) 365, 1040, 1050, 1427
HpyAV CCTTC 3 cut(s) 43, 193, 520
HpyCH4III ACNGT 2 cut(s) 421, 1404
HpyCH4IV ACGT 8 cut(s) 348, 725, 951, 1035, 1045, 1211, 1314, 1422
HpyCH4V TGCA 3 cut(s) 168, 633, 1408
HpyF10VI GCNNNNNNNGC 5 cut(s) 396, 639, 792, 1139, 1233
HpySE526I ACGT 8 cut(s) 348, 725, 951, 1035, 1045, 1211, 1314, 1422
Hsp92I GRCGYC 3 cut(s) 951, 1045, 1422
Hsp92II CATG 5 cut(s) 191, 230, 237, 516, 964
HspAI GCGC 2 cut(s) 903, 1193
KpnI GGTACC 1 cut(s) 621
Kzo9I GATC 1 cut(s) 291
LmnI GCTCC 4 cut(s) 35, 471, 529, 1230
Lsp1109I GCAGC 4 cut(s) 14, 180, 399, 1245
LweI GCATC 3 cut(s) 167, 374, 1285
MaeII ACGT 8 cut(s) 348, 725, 951, 1035, 1045, 1211, 1314, 1422
MaeIII GTNAC 5 cut(s) 316, 341, 415, 515, 911
MalI GATC 1 cut(s) 293
MbiI CCGCTC 2 cut(s) 967, 1227
MboI GATC 1 cut(s) 291
MboII GAAGA 4 cut(s) 84, 208, 745, 865
MflI RGATCY 1 cut(s) 291
MhlI GDGCHC 5 cut(s) 401, 434, 534, 824, 856
MlsI TGGCCA 2 cut(s) 765, 1319
MluCI AATT 1 cut(s) 580
MluNI TGGCCA 2 cut(s) 765, 1319
MlyI GAGTC 2 cut(s) 324, 481
MmeI TCCRAC 2 cut(s) 302, 1006
Mox20I TGGCCA 2 cut(s) 765, 1319
MscI TGGCCA 2 cut(s) 765, 1319
MseI TTAA 3 cut(s) 81, 210, 1158
MslI CAYNNNNRTG 2 cut(s) 225, 1333
Msp20I TGGCCA 2 cut(s) 765, 1319
MspA1I CMGCKG 2 cut(s) 1272, 1454
MspI CCGG 7 cut(s) 334, 384, 468, 804, 909, 1124, 1428
MspR9I CCNGG 5 cut(s) 334, 469, 805, 818, 1016
MvaI CCWGG 2 cut(s) 818, 1016
MvnI CGCG 2 cut(s) 987, 1117
MwoI GCNNNNNNNGC 5 cut(s) 396, 639, 792, 1139, 1233
NciI CCSGG 3 cut(s) 334, 469, 805
NdeII GATC 1 cut(s) 291
NlaIII CATG 5 cut(s) 191, 230, 237, 516, 964
NlaIV GGNNCC 5 cut(s) 531, 619, 822, 1003, 1087
NmuCI GTSAC 3 cut(s) 316, 341, 415
OliI CACNNNNGTG 1 cut(s) 1333
PagI TCATGA 1 cut(s) 187
PceI AGGCCT 1 cut(s) 786
PcsI WCGNNNNNNNCGW 1 cut(s) 1073
PfeI GAWTC 3 cut(s) 696, 1029, 1057
PfoI TCCNGGA 1 cut(s) 332
PinAI ACCGGT 1 cut(s) 908
PkrI GCNGC 7 cut(s) 29, 170, 389, 869, 935, 966, 1235
PleI GAGTC 2 cut(s) 323, 481
PmaCI CACGTG 1 cut(s) 1212
PmlI CACGTG 1 cut(s) 1212
PpsI GAGTC 2 cut(s) 323, 481
Ppu21I YACGTR 2 cut(s) 1212, 1315
PpuMI RGGWCCY 1 cut(s) 1002
Psp5II RGGWCCY 1 cut(s) 1002
Psp6I CCWGG 2 cut(s) 816, 1014
PspCI CACGTG 1 cut(s) 1212
PspEI GGTNACC 1 cut(s) 911
PspFI CCCAGC 3 cut(s) 249, 1349, 1450
PspGI CCWGG 2 cut(s) 816, 1014
PspN4I GGNNCC 5 cut(s) 531, 619, 822, 1003, 1087
PspOMI GGGCCC 1 cut(s) 820
PspPI GGNCC 7 cut(s) 230, 304, 814, 820, 821, 1002, 1086
PspPPI RGGWCCY 1 cut(s) 1002
PstI CTGCAG 1 cut(s) 170
PsuI RGATCY 1 cut(s) 291
PvuII CAGCTG 1 cut(s) 1454
RsaI GTAC 8 cut(s) 50, 121, 219, 619, 863, 992, 1136, 1247
RsaNI GTAC 8 cut(s) 49, 120, 218, 618, 862, 991, 1135, 1246
RseI CAYNNNNRTG 2 cut(s) 225, 1333
SaqAI TTAA 3 cut(s) 81, 210, 1158
SatI GCNGC 7 cut(s) 28, 169, 388, 868, 934, 965, 1234
Sau3AI GATC 1 cut(s) 291
Sau96I GGNCC 7 cut(s) 230, 304, 814, 820, 821, 1002, 1086
ScaI AGTACT 2 cut(s) 50, 121
SchI GAGTC 2 cut(s) 324, 481
ScrFI CCNGG 5 cut(s) 334, 469, 805, 818, 1016
SduI GDGCHC 5 cut(s) 401, 434, 534, 824, 856
SfaNI GCATC 3 cut(s) 167, 374, 1285
SfcI CTRYAG 1 cut(s) 166
SgrAI CRCCGGYG 1 cut(s) 1427
SinI GGWCC 4 cut(s) 230, 304, 1002, 1086
SmiMI CAYNNNNRTG 2 cut(s) 225, 1333
Sse9I AATT 1 cut(s) 580
SseBI AGGCCT 1 cut(s) 786
SspI AATATT 1 cut(s) 85
StuI AGGCCT 1 cut(s) 786
StyD4I CCNGG 5 cut(s) 332, 467, 803, 816, 1014
StyI CCWWGG 1 cut(s) 766
TaaI ACNGT 2 cut(s) 421, 1404
TaiI ACGT 8 cut(s) 351, 728, 954, 1038, 1048, 1214, 1317, 1425
TaqI TCGA 5 cut(s) 161, 741, 843, 1055, 1199
TasI AATT 1 cut(s) 580
TatI WGTACW 2 cut(s) 48, 119
TauI GCSGC 3 cut(s) 870, 936, 967
TfiI GAWTC 3 cut(s) 696, 1029, 1057
Tru1I TTAA 3 cut(s) 81, 210, 1158
Tru9I TTAA 3 cut(s) 81, 210, 1158
TscAI CASTG 2 cut(s) 19, 497
TseFI GTSAC 3 cut(s) 316, 341, 415
TseI GCWGC 4 cut(s) 27, 168, 387, 1233
Tsp45I GTSAC 3 cut(s) 316, 341, 415
TspDTI ATGAA 3 cut(s) 8, 949, 1184
TspGWI ACGGA 2 cut(s) 679, 746
TspRI CASTG 2 cut(s) 19, 497
VpaK11BI GGWCC 4 cut(s) 230, 304, 1002, 1086
XagI CCTNNNNNAGG 1 cut(s) 299
XmiI GTMKAC 2 cut(s) 563, 657
ZraI GACGTC 3 cut(s) 952, 1046, 1423
ZrmI AGTACT 2 cut(s) 50, 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.