Rorug04G0036400

Flavin containing amine oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000845
Physical Location & Seq
Forward (+)
5476839 .. 5490512
13674 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0036400.1

Sequence Viewer

Length: 2340 bp
ATGCTTGCTGGAGTGAAGTTCATACCTCGAGACCAGATTGATAAAGATGAGAATTTGAATGCTTCTGCGAAACAGAAACAGAAGAAGAGCTCAGGTAATAAGAAGGGTAAGCGTAGGAGGGAAAGGAGTTCTGGATATAGTAGTTCGGATGGCGAAGAGCTTGAGAGAATAAAGAAGGGATCTAAAAAAAAGTGGTATTCATCGGATGAGTACTCATCATCATCATATTCTTCGGGAAATGAAAGTAAGGAAAGTTCTGACCGAGATAGAAAGAAGTGTCTGAATAAGAAAAAGAAGAGATTGAGTAGCAGTAGAAAAGATTATAGTTCAGAAGATCTTTCATCTGCTTCTGATAGTTATTCTGATAGGAAAGACAAGCGCAAGAAGAGAAGCCGAAAAGATGGAAGGAAAAAGAGTAGAGATAGAAGTCAGGCAGGTATGGAGGATGATGAATATTCTGACGGTGGTAGAGGAACTAGTACGGTGAAAGATGGGGAAATTGTGAGGAAGAAAATTGGGTTGGAGTGGATGCTTAGACCTGAAGGAAAAGAGAGAACACCTACTATTACTGTCGAGGACCAGCCAAAAGAAACTCCAGTTGAAGAGACAAAGAAGGTCAATCCTAGGGAATTAAATCCATATTTTAAGGATGAGGGAAGTGGTTATCCAGAAGACTATGATGAACCTAAAGCTGGTGGGGACCGACTTTTACCTTCCTCTGTTGTTGGGGATGGAGGTGCAAGTTGGAGACTTAAAGCATTGAAGCGGGCAAAAGAGCAAGCAACTCGAGAAGGACAAGGGCTCCACGAGGTTGTTGAGGAGCGTTGGGGTTCTCTCGGTAAACTAGCGGCCTCTGTGGGATCAAATAAAGCAGCCCCATCTCGTGCTCATCTACATGCAATACAAAGTAGAAGGAAAGGGGTGAGTGAGGAACAGCAGCCAGGTTCCAACAATGACATGGATAAGAAGGACACTAGCCGGCCCTACCTAAAGGATGTATCTATCCGACATCCCAAAATGAAGGAGCCTAAAGTTCAAGGTTCTTTATCCTGGGGAAAGCAAAAGGGTCAAAAGAGTCGGGATGTCTCTACCAAGGACTCTGCTCTTCTAGCAGATGCAGTGTCTAGCATAAACAGGTTTTCCAATGATGGAAGCTTCCTGAGTGAACTTATTCGTAAGCAGACTAATGATTCTAGGAATCCTGTTAACGAAAATGTACTCGACACGAGAAAACCCAGCGAAGCCAGTGCTGCTGCCTCTAAGGGTGAACTGAGTGCGAACCAGTTGGCAGCTAAGGCTTTTCAGCTTCGTATGAAAGGGAAGCATGAAGAAGCTGAAGAACTTCTGCAAGAAGTAGAAAACCTAAAGGCAAAGCAGGGTGGTGGTGATAATCCGATGAGACCACAATATGAAAGAGGTACAAACAGATATGCTATGCAGGATATGTCTCTTTGTCAAAAGAAAAAGGATGATGATGGTGACATGCATCTAGCCAAAAAGATAATGCAGAACAAAAAATATAGTATATCTGGTCAGGCAGATGATGAATACGACTTTGATGATGGTCCAATGAGAAAGTCTAAAAAGAAGCAGGGAAGTGATGATCGCAAGGTCACCCAGAAGAATACATTTGCAAATCGTTTCTCCATTCAGCAAGAACGCTGCCTTTTCTGCTTTGAAAATCCAAAGAGACCAGCGCATCTGGTTGTGGCAATTGGAAATTATTCATATTTGATGTTGCCACAGCAGAAGCCTGTTGTGGATGGTCATTGCTACATTTTGCCGATGCAGCATGTACCAGCCACAAGAACTCTTGATGATCATGTGTGGGAAGAAATTCGAAATTTCAAGAAATGCCTTATTATGATGTTTGCGAAGCAAGAGAAGGATGTTGTCTTCCTTGAAACAGTGATGGGGTTAGCTCAACAGCGCCGCCACTGTATAAGCGAGTGTATTCCTTTGCCCCAGGATATAGCTAAAGACGCTCCATTATATTTTAAAAAGGCAATTGATGAAGCTGAAGATGAGTGGAGCCAACACAATGCCAAAAAACTTATTGATACAAGTGTAAAGGGACTGCGAGGTTCAGTTCCAGCTAATTTTCCATATTTCCACGTGGAGTTTAGTTTAAAGAAGGGGTTTGTCCACGTGATTGATGATGAAACACAGTTCAATAGCAGCCTTGGCCTGAATGTTATAAGAGGCATGCTTGAGTTGCCTGCAGAAGACATGCATCGCCGTGGACGATATGAGCCAGTGGAAGCGCAAAAGCAAGCCGTTAAGAGGTTTGATCAAGATTGGGCGCCGTTTGACTGGACAAAACAGCTTCATCAATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase NAD_binding_8

Protein Analysis

779

Amino Acids

88.44

Weight (kDa)

9.34

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CwfJ_C_1 PF04677 547 - 667 6e-42 Protein similar to CwfJ C-terminal
CwfJ_C_2 PF04676 676 - 773 8.5e-27 Protein similar to CwfJ C-terminus 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2198
Acc36I ACCTGC 1 cut(s) 425
AccB1I GGYRCC 1 cut(s) 2302
AciI CCGC 3 cut(s) 766, 848, 1933
AclWI GGATC 2 cut(s) 187, 868
AcsI RAATTY 3 cut(s) 52, 1836, 1843
AcuI CTGAAG 3 cut(s) 561, 1356, 2040
AcvI CACGTG 2 cut(s) 2116, 2149
AcyI GRCGYC 1 cut(s) 2303
AfaI GTAC 5 cut(s) 212, 481, 1218, 1420, 1797
AfiI CCNNNNNNNGG 2 cut(s) 692, 2283
AgsI TTSAA 8 cut(s) 58, 602, 763, 1037, 1679, 1849, 1904, 2173
AhlI ACTAGT 1 cut(s) 476
AjnI CCWGG 3 cut(s) 940, 1049, 1965
AjuI GAANNNNNNNTTGG 2 cut(s) 503, 535
Alw21I GWGCWC 2 cut(s) 92, 889
Alw26I GTCTC 7 cut(s) 24, 599, 742, 1090, 1393, 1452, 1684
AlwI GGATC 2 cut(s) 187, 868
Ama87I CYCGRG 2 cut(s) 27, 786
AoxI GGCC 3 cut(s) 849, 980, 2185
ApeKI GCWGC 8 cut(s) 872, 937, 1250, 1253, 1289, 1662, 1789, 2178
ApoI RAATTY 3 cut(s) 52, 1836, 1843
Asp700I GAANNNNTTC 4 cut(s) 1170, 1341, 1723, 1836
AspA2I CCTAGG 1 cut(s) 623
AspLEI GCGC 5 cut(s) 381, 1699, 1932, 2266, 2305
AspS9I GGNCC 4 cut(s) 577, 700, 981, 1565
AsuHPI GGTGA 6 cut(s) 496, 934, 1277, 1397, 1490, 1606
AsuII TTCGAA 1 cut(s) 1840
AvaI CYCGRG 2 cut(s) 27, 786
AvaII GGWCC 3 cut(s) 577, 700, 1565
AvrII CCTAGG 1 cut(s) 623
BaeI ACNNNNGTAYC 2 cut(s) 2052, 2085
BanI GGYRCC 1 cut(s) 2302
BanII GRGCYC 2 cut(s) 92, 804
BauI CACGAG 3 cut(s) 806, 882, 1225
BbrPI CACGTG 2 cut(s) 2116, 2149
BbsI GAAGAC 3 cut(s) 678, 1888, 2232
Bbv12I GWGCWC 2 cut(s) 92, 889
BbvI GCAGC 8 cut(s) 884, 949, 1237, 1240, 1301, 1649, 1801, 2190
BceAI ACGGC 3 cut(s) 2223, 2261, 2290
BcgI CGANNNNNNTGC 4 cut(s) 767, 801, 1229, 1263
BciT130I CCWGG 3 cut(s) 942, 1051, 1967
BclI TGATCA 2 cut(s) 1819, 2290
BcoDI GTCTC 7 cut(s) 24, 599, 742, 1090, 1393, 1452, 1684
BcuI ACTAGT 1 cut(s) 476
BfaI CTAG 8 cut(s) 477, 624, 845, 975, 1109, 1125, 1194, 1490
BfmI CTRYAG 1 cut(s) 2220
BfoI RGCGCY 2 cut(s) 1933, 2306
BfuAI ACCTGC 1 cut(s) 425
BglII AGATCT 1 cut(s) 334
BlnI CCTAGG 1 cut(s) 623
BmcAI AGTACT 1 cut(s) 212
Bme1390I CCNGG 3 cut(s) 942, 1051, 1967
Bme18I GGWCC 3 cut(s) 577, 700, 1565
BmeT110I CYCGRG 2 cut(s) 27, 786
BmgT120I GGNCC 4 cut(s) 577, 700, 981, 1565
BmiI GGNNCC 6 cut(s) 701, 803, 946, 1026, 2033, 2304
BmrFI CCNGG 3 cut(s) 942, 1051, 1967
BmsI GCATC 6 cut(s) 519, 1105, 1495, 1708, 1776, 2242
BpiI GAAGAC 3 cut(s) 678, 1888, 2232
BpmI CTGGAG 2 cut(s) 30, 579
Bpu10I CCTNAGC 2 cut(s) 91, 1293
Bpu14I TTCGAA 1 cut(s) 1840
BpuEI CTTGAG 2 cut(s) 182, 2231
BsaAI YACGTR 2 cut(s) 2116, 2149
BsaHI GRCGYC 1 cut(s) 2303
BsaI GGTCTC 3 cut(s) 24, 1393, 1684
BsaJI CCNNGG 6 cut(s) 623, 1050, 1092, 1965, 2182, 2239
BsaXI ACNNNNNCTCC 2 cut(s) 786, 816
Bsc4I CCNNNNNNNGG 2 cut(s) 692, 2283
Bse118I RCCGGY 1 cut(s) 978
Bse1I ACTGG 5 cut(s) 596, 1245, 1282, 2255, 2318
Bse3DI GCAATG 1 cut(s) 1768
BseBI CCWGG 3 cut(s) 942, 1051, 1967
BseDI CCNNGG 6 cut(s) 623, 1050, 1092, 1965, 2182, 2239
BseLI CCNNNNNNNGG 2 cut(s) 692, 2283
BseMI GCAATG 1 cut(s) 1768
BseMII CTCAG 3 cut(s) 105, 1151, 1262
BseNI ACTGG 5 cut(s) 596, 1245, 1282, 2255, 2318
BseRI GAGGAG 1 cut(s) 833
BseXI GCAGC 8 cut(s) 884, 949, 1237, 1240, 1301, 1649, 1801, 2190
BseYI CCCAGC 1 cut(s) 1235
BshFI GGCC 3 cut(s) 851, 982, 2187
BshNI GGYRCC 1 cut(s) 2302
BsiHKAI GWGCWC 2 cut(s) 92, 889
BsiHKCI CYCGRG 2 cut(s) 27, 786
BsiSI CCGG 1 cut(s) 979
BslFI GGGAC 2 cut(s) 713, 2088
BslI CCNNNNNNNGG 2 cut(s) 692, 2283
BsmAI GTCTC 7 cut(s) 24, 599, 742, 1090, 1393, 1452, 1684
BsmFI GGGAC 2 cut(s) 713, 2088
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 3 cut(s) 851, 982, 2187
Bso31I GGTCTC 3 cut(s) 24, 1393, 1684
BsoBI CYCGRG 2 cut(s) 27, 786
Bsp119I TTCGAA 1 cut(s) 1840
Bsp1286I GDGCHC 3 cut(s) 92, 804, 889
Bsp143I GATC 6 cut(s) 179, 334, 860, 1603, 1819, 2290
BspACI CCGC 3 cut(s) 766, 848, 1933
BspANI GGCC 3 cut(s) 851, 982, 2187
BspCNI CTCAG 3 cut(s) 104, 1152, 1263
BspLI GGNNCC 6 cut(s) 701, 803, 946, 1026, 2033, 2304
BspMAI CTGCAG 1 cut(s) 2224
BspMI ACCTGC 1 cut(s) 425
BspPI GGATC 2 cut(s) 187, 868
BspQI GCTCTTC 3 cut(s) 80, 150, 1110
BspT104I TTCGAA 1 cut(s) 1840
BspT107I GGYRCC 1 cut(s) 2302
BspTNI GGTCTC 3 cut(s) 24, 1393, 1684
BsrDI GCAATG 1 cut(s) 1768
BsrFI RCCGGY 1 cut(s) 978
BsrI ACTGG 5 cut(s) 596, 1245, 1282, 2255, 2318
BssAI RCCGGY 1 cut(s) 978
BssECI CCNNGG 6 cut(s) 623, 1050, 1092, 1965, 2182, 2239
BssMI GATC 6 cut(s) 179, 334, 860, 1603, 1819, 2290
BssNI GRCGYC 1 cut(s) 2303
BssSI CACGAG 3 cut(s) 806, 882, 1225
BssT1I CCWWGG 3 cut(s) 623, 1092, 2182
Bst2BI CACGAG 3 cut(s) 806, 882, 1225
Bst2UI CCWGG 3 cut(s) 942, 1051, 1967
Bst4CI ACNGT 6 cut(s) 464, 484, 571, 1909, 1940, 2169
Bst6I CTCTTC 6 cut(s) 80, 150, 290, 380, 597, 1110
BstACI GRCGYC 1 cut(s) 2303
BstBAI YACGTR 2 cut(s) 2116, 2149
BstBI TTCGAA 1 cut(s) 1840
BstC8I GCNNGC 7 cut(s) 6, 768, 780, 980, 2207, 2220, 2274
BstDEI CTNAG 6 cut(s) 91, 533, 1160, 1260, 1271, 1293
BstDSI CCRYGG 1 cut(s) 2239
BstEII GGTNACC 1 cut(s) 1612
BstH2I RGCGCY 2 cut(s) 1933, 2306
BstHHI GCGC 5 cut(s) 381, 1699, 1932, 2266, 2305
BstKTI GATC 6 cut(s) 182, 337, 863, 1606, 1822, 2293
BstMAI GTCTC 7 cut(s) 24, 599, 742, 1090, 1393, 1452, 1684
BstMBI GATC 6 cut(s) 179, 334, 860, 1603, 1819, 2290
BstMWI GCNNNNNNNGC 8 cut(s) 1109, 1250, 1295, 1671, 1789, 1982, 2184, 2215
BstNI CCWGG 3 cut(s) 942, 1051, 1967
BstNSI RCATGY 5 cut(s) 899, 1486, 1796, 2209, 2233
BstPI GGTNACC 1 cut(s) 1612
BstSCI CCNGG 3 cut(s) 940, 1049, 1965
BstSFI CTRYAG 1 cut(s) 2220
BstV1I GCAGC 8 cut(s) 884, 949, 1237, 1240, 1301, 1649, 1801, 2190
BstV2I GAAGAC 3 cut(s) 678, 1888, 2232
BstX2I RGATCY 2 cut(s) 179, 334
BstYI RGATCY 2 cut(s) 179, 334
BsuRI GGCC 3 cut(s) 851, 982, 2187
BtgI CCRYGG 1 cut(s) 2239
BtgZI GCGATG 1 cut(s) 2219
BtsI GCAGTG 1 cut(s) 1125
BtsIMutI CAGTG 5 cut(s) 1125, 1252, 1914, 1936, 2262
BveI ACCTGC 1 cut(s) 425
Cac8I GCNNGC 7 cut(s) 6, 768, 780, 980, 2207, 2220, 2274
CfoI GCGC 5 cut(s) 381, 1699, 1932, 2266, 2305
Cfr10I RCCGGY 1 cut(s) 978
Cfr13I GGNCC 4 cut(s) 577, 700, 981, 1565
CseI GACGC 1 cut(s) 1991
Csp6I GTAC 5 cut(s) 211, 480, 1217, 1419, 1796
CviAII CATG 8 cut(s) 896, 958, 1325, 1483, 1793, 1823, 2206, 2230
CviQI GTAC 5 cut(s) 211, 480, 1217, 1419, 1796
DdeI CTNAG 6 cut(s) 91, 533, 1160, 1260, 1271, 1293
DinI GGCGCC 1 cut(s) 2304
DpnI GATC 6 cut(s) 181, 336, 862, 1605, 1821, 2292
DpnII GATC 6 cut(s) 179, 334, 860, 1603, 1819, 2290
DraI TTTAAA 2 cut(s) 1999, 2130
Eam1104I CTCTTC 6 cut(s) 80, 150, 290, 380, 597, 1110
EarI CTCTTC 6 cut(s) 80, 150, 290, 380, 597, 1110
Ecl136II GAGCTC 1 cut(s) 90
Eco130I CCWWGG 3 cut(s) 623, 1092, 2182
Eco24I GRGCYC 2 cut(s) 92, 804
Eco31I GGTCTC 3 cut(s) 24, 1393, 1684
Eco47I GGWCC 3 cut(s) 577, 700, 1565
Eco53kI GAGCTC 1 cut(s) 90
Eco57I CTGAAG 3 cut(s) 561, 1356, 2040
Eco72I CACGTG 2 cut(s) 2116, 2149
Eco88I CYCGRG 2 cut(s) 27, 786
Eco91I GGTNACC 1 cut(s) 1612
EcoICRI GAGCTC 1 cut(s) 90
EcoO65I GGTNACC 1 cut(s) 1612
EcoRII CCWGG 3 cut(s) 940, 1049, 1965
EcoT14I CCWWGG 3 cut(s) 623, 1092, 2182
EcoT22I ATGCAT 2 cut(s) 1488, 2235
EcoT38I GRGCYC 2 cut(s) 92, 804
EgeI GGCGCC 1 cut(s) 2304
EheI GGCGCC 1 cut(s) 2304
ErhI CCWWGG 3 cut(s) 623, 1092, 2182
FaeI CATG 8 cut(s) 899, 961, 1328, 1486, 1796, 1826, 2209, 2233
FaqI GGGAC 2 cut(s) 713, 2088
FatI CATG 8 cut(s) 895, 957, 1324, 1482, 1792, 1822, 2205, 2229
FauI CCCGC 1 cut(s) 759
FbaI TGATCA 2 cut(s) 1819, 2290
FriOI GRGCYC 2 cut(s) 92, 804
FspBI CTAG 8 cut(s) 477, 624, 845, 975, 1109, 1125, 1194, 1490
GlaI GCGC 5 cut(s) 380, 1698, 1931, 2265, 2304
GsaI CCCAGC 1 cut(s) 1239
GsuI CTGGAG 2 cut(s) 30, 579
HaeII RGCGCY 2 cut(s) 1933, 2306
HaeIII GGCC 3 cut(s) 851, 982, 2187
HapII CCGG 1 cut(s) 979
HgaI GACGC 1 cut(s) 1991
HhaI GCGC 5 cut(s) 381, 1699, 1932, 2266, 2305
Hin1I GRCGYC 1 cut(s) 2303
Hin1II CATG 8 cut(s) 899, 961, 1328, 1486, 1796, 1826, 2209, 2233
Hin6I GCGC 5 cut(s) 379, 1697, 1930, 2264, 2303
HinP1I GCGC 5 cut(s) 379, 1697, 1930, 2264, 2303
HincII GTYRAC 1 cut(s) 1207
HindII GTYRAC 1 cut(s) 1207
HindIII AAGCTT 1 cut(s) 1153
HinfI GANTC 4 cut(s) 1075, 1097, 1190, 1198
HpaI GTTAAC 1 cut(s) 1207
HpaII CCGG 1 cut(s) 979
HphI GGTGA 6 cut(s) 496, 934, 1277, 1397, 1490, 1606
Hpy166II GTNNAC 6 cut(s) 842, 1166, 1207, 1268, 2146, 2243
Hpy8I GTNNAC 6 cut(s) 842, 1166, 1207, 1268, 2146, 2243
HpyCH4III ACNGT 6 cut(s) 464, 484, 571, 1909, 1940, 2169
HpyCH4IV ACGT 2 cut(s) 2115, 2148
HpyF10VI GCNNNNNNNGC 8 cut(s) 1109, 1250, 1295, 1671, 1789, 1982, 2184, 2215
HpyF3I CTNAG 6 cut(s) 91, 533, 1160, 1260, 1271, 1293
HpySE526I ACGT 2 cut(s) 2115, 2148
Hsp92I GRCGYC 1 cut(s) 2303
Hsp92II CATG 8 cut(s) 899, 961, 1328, 1486, 1796, 1826, 2209, 2233
HspAI GCGC 5 cut(s) 379, 1697, 1930, 2264, 2303
KasI GGCGCC 1 cut(s) 2302
KroI GCCGGC 1 cut(s) 978
KroNI GCCGGC 1 cut(s) 980
Ksp22I TGATCA 2 cut(s) 1819, 2290
KspAI GTTAAC 1 cut(s) 1207
Kzo9I GATC 6 cut(s) 179, 334, 860, 1603, 1819, 2290
LguI GCTCTTC 3 cut(s) 80, 150, 1110
LmnI GCTCC 5 cut(s) 807, 820, 1024, 1990, 2031
Lsp1109I GCAGC 8 cut(s) 884, 949, 1237, 1240, 1301, 1649, 1801, 2190
LweI GCATC 6 cut(s) 519, 1105, 1495, 1708, 1776, 2242
MaeI CTAG 8 cut(s) 477, 624, 845, 975, 1109, 1125, 1194, 1490
MaeII ACGT 2 cut(s) 2115, 2148
MaeIII GTNAC 2 cut(s) 1478, 1612
MalI GATC 6 cut(s) 181, 336, 862, 1605, 1821, 2292
MboI GATC 6 cut(s) 179, 334, 860, 1603, 1819, 2290
MfeI CAATTG 2 cut(s) 1713, 2007
MflI RGATCY 2 cut(s) 179, 334
MhlI GDGCHC 3 cut(s) 92, 804, 889
Mly113I GGCGCC 1 cut(s) 2303
MlyI GAGTC 2 cut(s) 1084, 1091
MmeI TCCRAC 4 cut(s) 501, 725, 972, 1030
Mph1103I ATGCAT 2 cut(s) 1488, 2235
MroNI GCCGGC 1 cut(s) 978
MroXI GAANNNNTTC 4 cut(s) 1170, 1341, 1723, 1836
MseI TTAA 7 cut(s) 632, 645, 753, 1206, 1998, 2129, 2280
MslI CAYNNNNRTG 2 cut(s) 894, 2238
MspI CCGG 1 cut(s) 979
MspR9I CCNGG 3 cut(s) 942, 1051, 1967
MunI CAATTG 2 cut(s) 1713, 2007
Mva1269I GAATGC 1 cut(s) 64
MvaI CCWGG 3 cut(s) 942, 1051, 1967
MwoI GCNNNNNNNGC 8 cut(s) 1109, 1250, 1295, 1671, 1789, 1982, 2184, 2215
NaeI GCCGGC 1 cut(s) 980
NarI GGCGCC 1 cut(s) 2303
NdeII GATC 6 cut(s) 179, 334, 860, 1603, 1819, 2290
NgoMIV GCCGGC 1 cut(s) 978
NlaIII CATG 8 cut(s) 899, 961, 1328, 1486, 1796, 1826, 2209, 2233
NlaIV GGNNCC 6 cut(s) 701, 803, 946, 1026, 2033, 2304
NmuCI GTSAC 2 cut(s) 1478, 1612
NsiI ATGCAT 2 cut(s) 1488, 2235
NspI RCATGY 5 cut(s) 899, 1486, 1796, 2209, 2233
NspV TTCGAA 1 cut(s) 1840
PaeI GCATGC 1 cut(s) 2209
PaeR7I CTCGAG 2 cut(s) 27, 786
PciSI GCTCTTC 3 cut(s) 80, 150, 1110
PcsI WCGNNNNNNNCGW 1 cut(s) 2242
PctI GAATGC 1 cut(s) 64
PdiI GCCGGC 1 cut(s) 980
PdmI GAANNNNTTC 4 cut(s) 1170, 1341, 1723, 1836
PfeI GAWTC 2 cut(s) 1190, 1198
PleI GAGTC 2 cut(s) 1083, 1091
PluTI GGCGCC 1 cut(s) 2306
PmaCI CACGTG 2 cut(s) 2116, 2149
PmlI CACGTG 2 cut(s) 2116, 2149
PpsI GAGTC 2 cut(s) 1083, 1091
Ppu21I YACGTR 2 cut(s) 2116, 2149
PsiI TTATAA 1 cut(s) 2198
Psp124BI GAGCTC 1 cut(s) 92
Psp6I CCWGG 3 cut(s) 940, 1049, 1965
PspCI CACGTG 2 cut(s) 2116, 2149
PspEI GGTNACC 1 cut(s) 1612
PspFI CCCAGC 1 cut(s) 1235
PspGI CCWGG 3 cut(s) 940, 1049, 1965
PspN4I GGNNCC 6 cut(s) 701, 803, 946, 1026, 2033, 2304
PspPI GGNCC 4 cut(s) 577, 700, 981, 1565
PsrI GAACNNNNNNTAC 2 cut(s) 238, 270
PstI CTGCAG 1 cut(s) 2224
PsuI RGATCY 2 cut(s) 179, 334
RsaI GTAC 5 cut(s) 212, 481, 1218, 1420, 1797
RsaNI GTAC 5 cut(s) 211, 480, 1217, 1419, 1796
RseI CAYNNNNRTG 2 cut(s) 894, 2238
SacI GAGCTC 1 cut(s) 92
SapI GCTCTTC 3 cut(s) 80, 150, 1110
SaqAI TTAA 7 cut(s) 632, 645, 753, 1206, 1998, 2129, 2280
Sau3AI GATC 6 cut(s) 179, 334, 860, 1603, 1819, 2290
Sau96I GGNCC 4 cut(s) 577, 700, 981, 1565
ScaI AGTACT 1 cut(s) 212
SchI GAGTC 2 cut(s) 1084, 1091
ScrFI CCNGG 3 cut(s) 942, 1051, 1967
SduI GDGCHC 3 cut(s) 92, 804, 889
SfaNI GCATC 6 cut(s) 519, 1105, 1495, 1708, 1776, 2242
SfcI CTRYAG 1 cut(s) 2220
SfoI GGCGCC 1 cut(s) 2304
Sfr274I CTCGAG 2 cut(s) 27, 786
SfuI TTCGAA 1 cut(s) 1840
SinI GGWCC 3 cut(s) 577, 700, 1565
SlaI CTCGAG 2 cut(s) 27, 786
SmiMI CAYNNNNRTG 2 cut(s) 894, 2238
SmlI CTYRAG 4 cut(s) 27, 161, 786, 2210
SmoI CTYRAG 4 cut(s) 27, 161, 786, 2210
SpeI ACTAGT 1 cut(s) 476
SphI GCATGC 1 cut(s) 2209
SsiI CCGC 3 cut(s) 766, 848, 1933
SspDI GGCGCC 1 cut(s) 2302
SspI AATATT 1 cut(s) 455
SspMI CTAG 8 cut(s) 477, 624, 845, 975, 1109, 1125, 1194, 1490
SstI GAGCTC 1 cut(s) 92
StyD4I CCNGG 3 cut(s) 940, 1049, 1965
StyI CCWWGG 3 cut(s) 623, 1092, 2182
TaaI ACNGT 6 cut(s) 464, 484, 571, 1909, 1940, 2169
TaiI ACGT 2 cut(s) 2118, 2151
TaqI TCGA 5 cut(s) 28, 573, 787, 1221, 1840
TaqII GACCGA 2 cut(s) 276, 717
TatI WGTACW 2 cut(s) 210, 1216
TauI GCSGC 2 cut(s) 851, 1935
TfiI GAWTC 2 cut(s) 1190, 1198
Tru1I TTAA 7 cut(s) 632, 645, 753, 1206, 1998, 2129, 2280
Tru9I TTAA 7 cut(s) 632, 645, 753, 1206, 1998, 2129, 2280
TscAI CASTG 5 cut(s) 1125, 1252, 1914, 1943, 2262
TseFI GTSAC 2 cut(s) 1478, 1612
TseI GCWGC 8 cut(s) 872, 937, 1250, 1253, 1289, 1662, 1789, 2178
Tsp45I GTSAC 2 cut(s) 1478, 1612
TspRI CASTG 5 cut(s) 1125, 1252, 1914, 1943, 2262
VpaK11BI GGWCC 3 cut(s) 577, 700, 1565
XapI RAATTY 3 cut(s) 52, 1836, 1843
XceI RCATGY 5 cut(s) 899, 1486, 1796, 2209, 2233
XcmI CCANNNNNNNNNTGG 1 cut(s) 955
XhoI CTCGAG 2 cut(s) 27, 786
XmaJI CCTAGG 1 cut(s) 623
XmnI GAANNNNTTC 4 cut(s) 1170, 1341, 1723, 1836
XspI CTAG 8 cut(s) 477, 624, 845, 975, 1109, 1125, 1194, 1490
ZrmI AGTACT 1 cut(s) 212
Zsp2I ATGCAT 2 cut(s) 1488, 2235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.