FvH4_4g33740

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001116
Physical Location & Seq
Reverse (-)
31847847 .. 31850136
2290 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33740.t1

Sequence Viewer

Length: 1089 bp
ATGGCGGCAAATGGTACCTTGATCGGCGACGAGGGTTACGATCGCGCCGGAGAAGTGAAGCAATTCGACGACTCCAAGCTCGGAGTCAAAGGCCTCGTCGACTCGGGTCTCTCCTCCATCCCCCGGATCTTTATCCACCCGCCCGAAACGTTACAGCAACTGAACCCGACGACCCGACCCGACGCCGAGCCCATCCCCACCATCGACCTCTCCGGCCCCCGCGCCGCAGTCGTCGACCAAGTCAGCGGCGCGTGCCGGAAGTTCGGGTTCTTCCAGATCACCAACCACGGCGTACCGTCGGTGGTTCTGGACCGCACCATCGCCGCCGTGAAAGCCTTCCACGAGCAGCCGGTGGAGGCCAAAGCGCGGTTCTACCGGAGAGAGATGGGCCACGGCGTGTCGTACCTCTCCAACGTCGACCTCTACACCTCCAAGGCCGCCAGCTGGCGCGACACGATTCAGGTGAGGCTGGGCCCGGTCGCGGCGGAGGGTGACGAGGTTCCGGAGATTTGTAGGGAGGAAATCGCGGCGTGGAACAAGGAGATCCAACGGCTGGGAGAGGAGATGCTCGGGATGGTCAGTGAAGGGCTGGGATTGCCCGCCGGGAAGCTCAAGGAGATGACGTGTCTGGAAGGGAGGATCATGGCGGGACACTACTACCCGCCCTGCCCGCAGCCTGACCTTACCGTAGGGCTGACGTCACACAGTGACCCGGGAGTGTTGACCGTGGTGCTTCAGGATCACATTGGTGGGTTGCAGGTCAAGCACGGCGATGCTTGGGTGGATGTGAAGCCCGTCCCCGGTGCTCTTGTGATCAACGTTGGTGATTTACTGCAGATTATATCAAATGAGGAATACAAGAGCGTGGATCACCGGGTGTTGGCGAATCCGAGCGACGAGCCGCGAGTCTCGATTGCCGTGTTTTTGAACCCGAGCAACAGGGAGGGGGTGTTTGGTCCGTTGCCGGAGCTGGTCTCGCCGGAGAAGCCAGCGCTATACAAAAAGTTTACGTTTTCAGAGTTCATGATGAGGTTCTTCACGAAAGGATTGGAAGGCAAAACTTTGGTGAATTACTATAAGCTGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

363

Amino Acids

39.66

Weight (kDa)

5.56

Isoelectric Point (pI)

36.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 65 - 156 1.2e-17 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 214 - 311 8.9e-33 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 701
Acc36I ACCTGC 1 cut(s) 748
Acc65I GGTACC 1 cut(s) 14
AccB1I GGYRCC 1 cut(s) 14
AccB7I CCANNNNNTGG 1 cut(s) 553
AccI GTMKAC 3 cut(s) 99, 234, 417
AccII CGCG 8 cut(s) 45, 222, 251, 367, 450, 482, 527, 904
AccIII TCCGGA 1 cut(s) 502
AclI AACGTT 2 cut(s) 149, 819
AclWI GGATC 5 cut(s) 134, 538, 647, 747, 876
AcuI CTGAAG 1 cut(s) 719
AcyI GRCGYC 2 cut(s) 183, 698
AdeI CACNNNGTG 3 cut(s) 397, 707, 877
AfaI GTAC 3 cut(s) 16, 294, 404
AfeI AGCGCT 1 cut(s) 993
AfiI CCNNNNNNNGG 7 cut(s) 123, 366, 444, 481, 553, 800, 880
AflIII ACRYGT 1 cut(s) 623
AgsI TTSAA 1 cut(s) 928
AjiI CACGTC 1 cut(s) 624
AjuI GAANNNNNNNTTGG 2 cut(s) 353, 385
AleI CACNNNNGTG 1 cut(s) 747
AluBI AGCT 5 cut(s) 79, 444, 610, 970, 1081
AluI AGCT 5 cut(s) 79, 444, 610, 970, 1081
Alw21I GWGCWC 1 cut(s) 808
Alw26I GTCTC 3 cut(s) 113, 913, 979
AlwI GGATC 5 cut(s) 134, 538, 647, 747, 876
AlwNI CAGNNNCTG 1 cut(s) 160
Ama87I CYCGRG 4 cut(s) 103, 569, 712, 931
Aor13HI TCCGGA 1 cut(s) 502
Aor51HI AGCGCT 1 cut(s) 993
AoxI GGCC 6 cut(s) 91, 214, 357, 388, 435, 472
ApaI GGGCCC 1 cut(s) 476
ApeKI GCWGC 3 cut(s) 346, 673, 1081
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
Asp700I GAANNNNTTC 2 cut(s) 62, 335
Asp718I GGTACC 1 cut(s) 14
AspLEI GCGC 6 cut(s) 47, 224, 251, 367, 450, 994
AspS9I GGNCC 6 cut(s) 215, 310, 388, 472, 473, 956
AsuC2I CCSGG 7 cut(s) 124, 476, 604, 713, 714, 801, 875
AsuHPI GGTGA 6 cut(s) 271, 475, 503, 836, 863, 1078
AvaI CYCGRG 4 cut(s) 103, 569, 712, 931
AvaII GGWCC 2 cut(s) 310, 956
BaeGI GKGCMC 1 cut(s) 476
BanI GGYRCC 1 cut(s) 14
BanII GRGCYC 2 cut(s) 192, 476
BauI CACGAG 1 cut(s) 341
Bbv12I GWGCWC 1 cut(s) 808
BbvI GCAGC 3 cut(s) 358, 685, 1068
BccI CCATC 6 cut(s) 125, 200, 209, 326, 379, 568
BceAI ACGGC 6 cut(s) 304, 311, 409, 566, 784, 902
BclI TGATCA 1 cut(s) 813
BcnI CCSGG 7 cut(s) 124, 476, 604, 713, 714, 801, 875
BcoDI GTCTC 3 cut(s) 113, 913, 979
BfmI CTRYAG 2 cut(s) 833, 1082
BfoI RGCGCY 1 cut(s) 995
BfuAI ACCTGC 1 cut(s) 748
Bme1390I CCNGG 7 cut(s) 124, 476, 604, 713, 714, 801, 875
Bme18I GGWCC 2 cut(s) 310, 956
BmeT110I CYCGRG 4 cut(s) 103, 569, 712, 931
BmgBI CACGTC 1 cut(s) 624
BmgT120I GGNCC 6 cut(s) 215, 310, 388, 472, 473, 956
BmiI GGNNCC 4 cut(s) 16, 217, 474, 501
BmrFI CCNGG 7 cut(s) 124, 476, 604, 713, 714, 801, 875
BmsI GCATC 2 cut(s) 555, 763
BoxI GACNNNNGTC 1 cut(s) 105
BplI GAGNNNNNCTC 2 cut(s) 959, 991
BpuEI CTTGAG 1 cut(s) 596
BpuMI CCSGG 7 cut(s) 124, 476, 604, 713, 714, 801, 875
BsaBI GATNNNNATC 1 cut(s) 131
BsaHI GRCGYC 2 cut(s) 183, 698
BsaI GGTCTC 2 cut(s) 113, 979
BsaJI CCNNGG 7 cut(s) 122, 286, 391, 432, 712, 726, 799
BsaWI WCCGGW 2 cut(s) 375, 502
BsaXI ACNNNNNCTCC 4 cut(s) 608, 638, 935, 965
Bsc4I CCNNNNNNNGG 7 cut(s) 123, 366, 444, 481, 553, 800, 880
Bse118I RCCGGY 1 cut(s) 349
Bse8I GATNNNNATC 1 cut(s) 131
BseAI TCCGGA 1 cut(s) 502
BseDI CCNNGG 7 cut(s) 122, 286, 391, 432, 712, 726, 799
BseGI GGATG 4 cut(s) 117, 192, 579, 790
BseJI GATNNNNATC 1 cut(s) 131
BseLI CCNNNNNNNGG 7 cut(s) 123, 366, 444, 481, 553, 800, 880
BseRI GAGGAG 2 cut(s) 103, 575
BseSI GKGCMC 1 cut(s) 476
BseXI GCAGC 3 cut(s) 358, 685, 1068
BseYI CCCAGC 3 cut(s) 469, 553, 589
Bsh1236I CGCG 8 cut(s) 45, 222, 251, 367, 450, 482, 527, 904
Bsh1285I CGRYCG 2 cut(s) 43, 480
BshFI GGCC 6 cut(s) 93, 216, 359, 390, 437, 474
BshNI GGYRCC 1 cut(s) 14
BsiEI CGRYCG 2 cut(s) 43, 480
BsiHKAI GWGCWC 1 cut(s) 808
BsiHKCI CYCGRG 4 cut(s) 103, 569, 712, 931
BslFI GGGAC 2 cut(s) 663, 782
BslI CCNNNNNNNGG 7 cut(s) 123, 366, 444, 481, 553, 800, 880
BsmAI GTCTC 3 cut(s) 113, 913, 979
BsmFI GGGAC 2 cut(s) 663, 782
BsnI GGCC 6 cut(s) 93, 216, 359, 390, 437, 474
Bso31I GGTCTC 2 cut(s) 113, 979
BsoBI CYCGRG 4 cut(s) 103, 569, 712, 931
Bsp120I GGGCCC 1 cut(s) 472
Bsp1286I GDGCHC 3 cut(s) 192, 476, 808
Bsp13I TCCGGA 1 cut(s) 502
Bsp143I GATC 9 cut(s) 21, 40, 126, 276, 543, 639, 739, 813, 868
BspANI GGCC 6 cut(s) 93, 216, 359, 390, 437, 474
BspEI TCCGGA 1 cut(s) 502
BspFNI CGCG 8 cut(s) 45, 222, 251, 367, 450, 482, 527, 904
BspHI TCATGA 1 cut(s) 1023
BspLI GGNNCC 4 cut(s) 16, 217, 474, 501
BspMAI CTGCAG 2 cut(s) 837, 1086
BspMI ACCTGC 1 cut(s) 748
BspPI GGATC 5 cut(s) 134, 538, 647, 747, 876
BspT107I GGYRCC 1 cut(s) 14
BspTNI GGTCTC 2 cut(s) 113, 979
BsrFI RCCGGY 1 cut(s) 349
BssAI RCCGGY 1 cut(s) 349
BssECI CCNNGG 7 cut(s) 122, 286, 391, 432, 712, 726, 799
BssMI GATC 9 cut(s) 21, 40, 126, 276, 543, 639, 739, 813, 868
BssNI GRCGYC 2 cut(s) 183, 698
BssSI CACGAG 1 cut(s) 341
BssT1I CCWWGG 1 cut(s) 432
Bst2BI CACGAG 1 cut(s) 341
Bst4CI ACNGT 4 cut(s) 297, 688, 707, 727
BstACI GRCGYC 2 cut(s) 183, 698
BstC8I GCNNGC 6 cut(s) 253, 442, 446, 600, 671, 990
BstDSI CCRYGG 3 cut(s) 286, 391, 726
BstF5I GGATG 4 cut(s) 117, 192, 579, 790
BstFNI CGCG 8 cut(s) 45, 222, 251, 367, 450, 482, 527, 904
BstH2I RGCGCY 1 cut(s) 995
BstHHI GCGC 6 cut(s) 47, 224, 251, 367, 450, 994
BstKTI GATC 9 cut(s) 24, 43, 129, 279, 546, 642, 742, 816, 871
BstMAI GTCTC 3 cut(s) 113, 913, 979
BstMBI GATC 9 cut(s) 21, 40, 126, 276, 543, 639, 739, 813, 868
BstMCI CGRYCG 2 cut(s) 43, 480
BstMWI GCNNNNNNNGC 7 cut(s) 252, 332, 595, 670, 763, 976, 985
BstPAI GACNNNNGTC 1 cut(s) 105
BstSCI CCNGG 7 cut(s) 122, 474, 602, 711, 712, 799, 873
BstSFI CTRYAG 2 cut(s) 833, 1082
BstSLI GKGCMC 1 cut(s) 476
BstUI CGCG 8 cut(s) 45, 222, 251, 367, 450, 482, 527, 904
BstV1I GCAGC 3 cut(s) 358, 685, 1068
BstX2I RGATCY 2 cut(s) 126, 543
BstYI RGATCY 2 cut(s) 126, 543
BsuRI GGCC 6 cut(s) 93, 216, 359, 390, 437, 474
BtgI CCRYGG 3 cut(s) 286, 391, 726
BtgZI GCGATG 2 cut(s) 304, 786
BtrI CACGTC 1 cut(s) 624
BtsCI GGATG 4 cut(s) 117, 192, 579, 790
BtsIMutI CAGTG 2 cut(s) 586, 712
BveI ACCTGC 1 cut(s) 748
Cac8I GCNNGC 6 cut(s) 253, 442, 446, 600, 671, 990
CaiI CAGNNNCTG 1 cut(s) 160
CciI TCATGA 1 cut(s) 1023
CfoI GCGC 6 cut(s) 47, 224, 251, 367, 450, 994
Cfr10I RCCGGY 1 cut(s) 349
Cfr13I GGNCC 6 cut(s) 215, 310, 388, 472, 473, 956
Cfr9I CCCGGG 1 cut(s) 712
CseI GACGC 1 cut(s) 191
Csp6I GTAC 3 cut(s) 15, 293, 403
CviAII CATG 2 cut(s) 643, 1024
CviQI GTAC 3 cut(s) 15, 293, 403
DpnI GATC 9 cut(s) 23, 42, 128, 278, 545, 641, 741, 815, 870
DpnII GATC 9 cut(s) 21, 40, 126, 276, 543, 639, 739, 813, 868
DraIII CACNNNGTG 3 cut(s) 397, 707, 877
EciI GGCGGA 1 cut(s) 500
Eco130I CCWWGG 1 cut(s) 432
Eco147I AGGCCT 1 cut(s) 93
Eco24I GRGCYC 2 cut(s) 192, 476
Eco31I GGTCTC 2 cut(s) 113, 979
Eco47I GGWCC 2 cut(s) 310, 956
Eco47III AGCGCT 1 cut(s) 993
Eco57I CTGAAG 1 cut(s) 719
Eco88I CYCGRG 4 cut(s) 103, 569, 712, 931
EcoT14I CCWWGG 1 cut(s) 432
EcoT38I GRGCYC 2 cut(s) 192, 476
ErhI CCWWGG 1 cut(s) 432
FaeI CATG 2 cut(s) 646, 1027
FaiI YATR 5 cut(s) 644, 842, 997, 1025, 1077
FaqI GGGAC 2 cut(s) 663, 782
FatI CATG 2 cut(s) 642, 1023
FauI CCCGC 6 cut(s) 147, 227, 607, 640, 669, 678
FbaI TGATCA 1 cut(s) 813
FblI GTMKAC 3 cut(s) 99, 234, 417
FokI GGATG 4 cut(s) 104, 179, 586, 797
FriOI GRGCYC 2 cut(s) 192, 476
GlaI GCGC 6 cut(s) 46, 223, 250, 366, 449, 993
GsaI CCCAGC 3 cut(s) 473, 557, 593
HaeII RGCGCY 1 cut(s) 995
HaeIII GGCC 6 cut(s) 93, 216, 359, 390, 437, 474
HgaI GACGC 1 cut(s) 191
HhaI GCGC 6 cut(s) 47, 224, 251, 367, 450, 994
Hin1I GRCGYC 2 cut(s) 183, 698
Hin1II CATG 2 cut(s) 646, 1027
Hin6I GCGC 6 cut(s) 45, 222, 249, 365, 448, 992
HinP1I GCGC 6 cut(s) 45, 222, 249, 365, 448, 992
HincII GTYRAC 4 cut(s) 100, 235, 418, 723
HindII GTYRAC 4 cut(s) 100, 235, 418, 723
HinfI GANTC 6 cut(s) 71, 84, 101, 457, 886, 906
HphI GGTGA 6 cut(s) 271, 475, 503, 836, 863, 1078
Hpy166II GTNNAC 5 cut(s) 100, 235, 418, 723, 1008
Hpy188I TCNGA 3 cut(s) 83, 891, 1018
Hpy188III TCNNGA 9 cut(s) 274, 308, 503, 571, 629, 737, 910, 1024, 1039
Hpy8I GTNNAC 5 cut(s) 100, 235, 418, 723, 1008
Hpy99I CGWCG 9 cut(s) 32, 71, 101, 172, 185, 236, 301, 419, 899
HpyAV CCTTC 4 cut(s) 346, 578, 626, 1046
HpyCH4III ACNGT 4 cut(s) 297, 688, 707, 727
HpyCH4IV ACGT 6 cut(s) 149, 414, 623, 698, 819, 1010
HpyCH4V TGCA 3 cut(s) 757, 835, 1084
HpyF10VI GCNNNNNNNGC 7 cut(s) 252, 332, 595, 670, 763, 976, 985
HpySE526I ACGT 6 cut(s) 149, 414, 623, 698, 819, 1010
Hsp92I GRCGYC 2 cut(s) 183, 698
Hsp92II CATG 2 cut(s) 646, 1027
HspAI GCGC 6 cut(s) 45, 222, 249, 365, 448, 992
Kpn2I TCCGGA 1 cut(s) 502
KpnI GGTACC 1 cut(s) 18
Ksp22I TGATCA 1 cut(s) 813
Kzo9I GATC 9 cut(s) 21, 40, 126, 276, 543, 639, 739, 813, 868
LmnI GCTCC 1 cut(s) 967
Lsp1109I GCAGC 3 cut(s) 358, 685, 1068
LweI GCATC 2 cut(s) 555, 763
MaeII ACGT 6 cut(s) 149, 414, 623, 698, 819, 1010
MaeIII GTNAC 5 cut(s) 35, 150, 491, 699, 707
MalI GATC 9 cut(s) 23, 42, 128, 278, 545, 641, 741, 815, 870
MboI GATC 9 cut(s) 21, 40, 126, 276, 543, 639, 739, 813, 868
MboII GAAGA 2 cut(s) 262, 1027
MflI RGATCY 2 cut(s) 126, 543
MhlI GDGCHC 3 cut(s) 192, 476, 808
MluCI AATT 2 cut(s) 62, 1069
MlyI GAGTC 4 cut(s) 65, 93, 95, 915
MmeI TCCRAC 2 cut(s) 435, 571
MroI TCCGGA 1 cut(s) 502
MroXI GAANNNNTTC 2 cut(s) 62, 335
MslI CAYNNNNRTG 2 cut(s) 747, 771
MspA1I CMGCKG 2 cut(s) 246, 444
MspR9I CCNGG 7 cut(s) 124, 476, 604, 713, 714, 801, 875
MvnI CGCG 8 cut(s) 45, 222, 251, 367, 450, 482, 527, 904
MwoI GCNNNNNNNGC 7 cut(s) 252, 332, 595, 670, 763, 976, 985
NciI CCSGG 7 cut(s) 124, 476, 604, 713, 714, 801, 875
NdeII GATC 9 cut(s) 21, 40, 126, 276, 543, 639, 739, 813, 868
NlaIII CATG 2 cut(s) 646, 1027
NlaIV GGNNCC 4 cut(s) 16, 217, 474, 501
NmeAIII GCCGAG 1 cut(s) 211
NmuCI GTSAC 3 cut(s) 491, 699, 707
OliI CACNNNNGTG 1 cut(s) 747
PagI TCATGA 1 cut(s) 1023
PceI AGGCCT 1 cut(s) 93
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PdmI GAANNNNTTC 2 cut(s) 62, 335
PfeI GAWTC 2 cut(s) 457, 886
PflFI GACNNNGTC 1 cut(s) 239
PflMI CCANNNNNTGG 1 cut(s) 553
Ple19I CGATCG 1 cut(s) 43
PleI GAGTC 4 cut(s) 65, 92, 95, 914
PpsI GAGTC 4 cut(s) 65, 92, 95, 914
PshAI GACNNNNGTC 1 cut(s) 105
Psp1406I AACGTT 2 cut(s) 149, 819
PspFI CCCAGC 3 cut(s) 469, 553, 589
PspN4I GGNNCC 4 cut(s) 16, 217, 474, 501
PspOMI GGGCCC 1 cut(s) 472
PspPI GGNCC 6 cut(s) 215, 310, 388, 472, 473, 956
PstI CTGCAG 2 cut(s) 837, 1086
PstNI CAGNNNCTG 1 cut(s) 160
PsuI RGATCY 2 cut(s) 126, 543
PsyI GACNNNGTC 1 cut(s) 239
PvuI CGATCG 1 cut(s) 43
PvuII CAGCTG 1 cut(s) 444
RsaI GTAC 3 cut(s) 16, 294, 404
RsaNI GTAC 3 cut(s) 15, 293, 403
RseI CAYNNNNRTG 2 cut(s) 747, 771
SalI GTCGAC 3 cut(s) 98, 233, 416
Sau3AI GATC 9 cut(s) 21, 40, 126, 276, 543, 639, 739, 813, 868
Sau96I GGNCC 6 cut(s) 215, 310, 388, 472, 473, 956
SchI GAGTC 4 cut(s) 65, 93, 95, 915
ScrFI CCNGG 7 cut(s) 124, 476, 604, 713, 714, 801, 875
SduI GDGCHC 3 cut(s) 192, 476, 808
SfaNI GCATC 2 cut(s) 555, 763
SfcI CTRYAG 2 cut(s) 833, 1082
SinI GGWCC 2 cut(s) 310, 956
SmaI CCCGGG 1 cut(s) 714
SmiMI CAYNNNNRTG 2 cut(s) 747, 771
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
Sse9I AATT 2 cut(s) 62, 1069
SseBI AGGCCT 1 cut(s) 93
StuI AGGCCT 1 cut(s) 93
StyD4I CCNGG 7 cut(s) 122, 474, 602, 711, 712, 799, 873
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 4 cut(s) 297, 688, 707, 727
TaiI ACGT 6 cut(s) 152, 417, 626, 701, 822, 1013
TaqI TCGA 6 cut(s) 66, 99, 204, 234, 417, 911
TasI AATT 2 cut(s) 62, 1069
TauI GCSGC 8 cut(s) 8, 227, 249, 326, 440, 485, 530, 904
TfiI GAWTC 2 cut(s) 457, 886
TscAI CASTG 2 cut(s) 586, 712
TseFI GTSAC 3 cut(s) 491, 699, 707
TseI GCWGC 3 cut(s) 346, 673, 1081
Tsp45I GTSAC 3 cut(s) 491, 699, 707
TspDTI ATGAA 1 cut(s) 1012
TspGWI ACGGA 1 cut(s) 948
TspMI CCCGGG 1 cut(s) 712
TspRI CASTG 2 cut(s) 586, 712
Tth111I GACNNNGTC 1 cut(s) 239
Van91I CCANNNNNTGG 1 cut(s) 553
VpaK11BI GGWCC 2 cut(s) 310, 956
XmaI CCCGGG 1 cut(s) 712
XmiI GTMKAC 3 cut(s) 99, 234, 417
XmnI GAANNNNTTC 2 cut(s) 62, 335
ZraI GACGTC 1 cut(s) 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.