Rroxscaffold_5G00383850

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001116
Physical Location & Seq
Reverse (-)
63402975 .. 63404252
1278 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00383850.1

Sequence Viewer

Length: 1104 bp
ATGGCGGCAAATAGTACCTTGATCGGCGAACAGGGTTATGATCGAGCTGAAGAAGTGAAGCAATTCGACGAGTCCAAGCTCGGAGTCAAAGGCCTCGTCGACTCGGGTCTCTCCTGCATCCCCCGAATCTTTATCCACCCGCCCGAAACATTACAGCAATTGCAGCCCACGACCCGACCCGACGCCCAACCAATCCCCACCATCGACCTCTCCGACCTCCACTCTGATCGCCGCTCTGCCATCGTCCACCAAGTCAGCGGCGCGTGCCGCAACTTCGGTTTCTTCCAGATCACCAACCATGGCATACCGTCGGTGGTTCTCGACAGTACGATCGCCGCCGTCAAGGCCTTCCACGAGCAGCCGACGGAAGTCAAAGCGCGGTTCTACCGTAGAGAGATGATAGGCCAGGGCGTAGCGTATTTCTCCAACGTCGACCTGTACAAGTCCAAAGCCGCCAGCTGGCGCGACACGCTTCAGGTGAGGTTCGGTCCTGTGCCGGCGGCCACCGACGACGTTCCGGAGATTTGTAGGGACGAAATTGTGAAGTGGAACGAGGAGATCCAACGGCTGGGAGAGGAGCTTCTGGGGCTGGTGAGTGAAGGGCTGGGATTGCGCGCGGAGAAGCTGAAGGAGATGAAGTGTCTGGAAGGAAGGGTGATGGCGGGCCACTACTACCCGCCCTGCCCGCAGCCCCATCTAACGGTTGGGCTTATTTCACACAGTGACCCTGGAGTATTGACCGTGCTGCTTCAAGATCATATTGGTGGGTTGCAGGTTAAGCACGATGGTGCTTGGGTGGATGTGAAACCTCTCCCCGGTGCTCTCGTGATCAACGTTGGTGATGTACTCCGGATTATTTCGAACGAGGAATACAAAAGCGTGGATCACCGGGTGTTGGCGAATCCACACCACGAGGCGCGTCTCTCAATAGCTGTGCTTTTGAATCCGAGCAATAGAGAGGGCGTGTTTGGTCCATTGCCGGAGCTGGTCTCGCCGGAGAAGCCAGCTCTCTACAAGGAGTTCAAGTTCTCGGAATTCCTGATGAGGTTCTTCCAGAAAGAATTGAATGACAAATCTTTGGTGAATTACTTTAAGCTGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

367

Amino Acids

41.0

Weight (kDa)

6.07

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 65 - 162 2.3e-19 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 219 - 316 8.6e-32 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 763
AccB7I CCANNNNNTGG 1 cut(s) 568
AccBSI CCGCTC 1 cut(s) 234
AccI GTMKAC 2 cut(s) 99, 432
AccII CGCG 6 cut(s) 263, 379, 465, 615, 617, 919
AccIII TCCGGA 2 cut(s) 517, 849
AclI AACGTT 1 cut(s) 834
AclWI GGATC 2 cut(s) 553, 891
AcoI YGGCCR 1 cut(s) 501
AcsI RAATTY 1 cut(s) 1034
AcuI CTGAAG 3 cut(s) 69, 458, 647
AcyI GRCGYC 1 cut(s) 183
AdeI CACNNNGTG 2 cut(s) 722, 892
AfaI GTAC 4 cut(s) 16, 328, 440, 846
AfiI CCNNNNNNNGG 5 cut(s) 459, 568, 700, 815, 895
AgsI TTSAA 4 cut(s) 752, 943, 1024, 1066
AjnI CCWGG 2 cut(s) 405, 727
AleI CACNNNNGTG 1 cut(s) 786
AloI GAACNNNNNNTCC 2 cut(s) 1010, 1042
AluBI AGCT 9 cut(s) 47, 79, 459, 580, 625, 932, 985, 1007, 1096
AluI AGCT 9 cut(s) 47, 79, 459, 580, 625, 932, 985, 1007, 1096
Alw21I GWGCWC 1 cut(s) 823
Alw26I GTCTC 3 cut(s) 113, 926, 994
AlwI GGATC 2 cut(s) 553, 891
Ama87I CYCGRG 1 cut(s) 103
Aor13HI TCCGGA 2 cut(s) 517, 849
AoxI GGCC 5 cut(s) 91, 345, 403, 501, 664
ApeKI GCWGC 5 cut(s) 163, 358, 688, 745, 1096
ApoI RAATTY 1 cut(s) 1034
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
Asp700I GAANNNNTTC 1 cut(s) 62
AspLEI GCGC 6 cut(s) 263, 379, 465, 615, 617, 919
AspS9I GGNCC 3 cut(s) 488, 664, 971
AsuC2I CCSGG 2 cut(s) 816, 890
AsuHPI GGTGA 7 cut(s) 283, 490, 604, 667, 851, 878, 1093
AsuII TTCGAA 1 cut(s) 860
AvaI CYCGRG 1 cut(s) 103
AvaII GGWCC 2 cut(s) 488, 971
BauI CACGAG 3 cut(s) 353, 824, 911
Bbv12I GWGCWC 1 cut(s) 823
BbvI GCAGC 5 cut(s) 175, 370, 700, 732, 1083
BccI CCATC 5 cut(s) 209, 248, 652, 702, 779
BceAI ACGGC 2 cut(s) 323, 581
BciT130I CCWGG 2 cut(s) 407, 729
BclI TGATCA 1 cut(s) 828
BcnI CCSGG 2 cut(s) 816, 890
BcoDI GTCTC 3 cut(s) 113, 926, 994
BfuAI ACCTGC 1 cut(s) 763
BglI GCCNNNNNGGC 1 cut(s) 344
Bme1390I CCNGG 4 cut(s) 407, 729, 816, 890
Bme18I GGWCC 2 cut(s) 488, 971
BmeT110I CYCGRG 1 cut(s) 103
BmgT120I GGNCC 3 cut(s) 488, 664, 971
BmrFI CCNGG 4 cut(s) 407, 729, 816, 890
BmsI GCATC 1 cut(s) 126
BoxI GACNNNNGTC 2 cut(s) 105, 368
BplI GAGNNNNNCTC 2 cut(s) 974, 1006
BpmI CTGGAG 1 cut(s) 750
Bpu14I TTCGAA 1 cut(s) 860
BpuMI CCSGG 2 cut(s) 816, 890
BsaHI GRCGYC 1 cut(s) 183
BsaI GGTCTC 2 cut(s) 113, 994
BsaJI CCNNGG 4 cut(s) 298, 406, 727, 814
BsaWI WCCGGW 2 cut(s) 517, 849
BsaXI ACNNNNNCTCC 4 cut(s) 623, 653, 1010, 1040
Bsc4I CCNNNNNNNGG 5 cut(s) 459, 568, 700, 815, 895
Bse118I RCCGGY 1 cut(s) 496
Bse3DI GCAATG 1 cut(s) 974
BseAI TCCGGA 2 cut(s) 517, 849
BseBI CCWGG 2 cut(s) 407, 729
BseDI CCNNGG 4 cut(s) 298, 406, 727, 814
BseGI GGATG 2 cut(s) 117, 805
BseLI CCNNNNNNNGG 5 cut(s) 459, 568, 700, 815, 895
BseMI GCAATG 1 cut(s) 974
BsePI GCGCGC 1 cut(s) 613
BseRI GAGGAG 2 cut(s) 569, 590
BseXI GCAGC 5 cut(s) 175, 370, 700, 732, 1083
BseYI CCCAGC 2 cut(s) 568, 604
BsgI GTGCAG 1 cut(s) 1082
Bsh1236I CGCG 6 cut(s) 263, 379, 465, 615, 617, 919
Bsh1285I CGRYCG 1 cut(s) 333
BshFI GGCC 5 cut(s) 93, 347, 405, 503, 666
BsiEI CGRYCG 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 823
BsiHKCI CYCGRG 1 cut(s) 103
BsiSI CCGG 7 cut(s) 497, 518, 816, 850, 889, 980, 995
BslFI GGGAC 1 cut(s) 545
BslI CCNNNNNNNGG 5 cut(s) 459, 568, 700, 815, 895
BsmAI GTCTC 3 cut(s) 113, 926, 994
BsmBI CGTCTC 1 cut(s) 926
BsmFI GGGAC 1 cut(s) 545
BsnI GGCC 5 cut(s) 93, 347, 405, 503, 666
Bso31I GGTCTC 2 cut(s) 113, 994
BsoBI CYCGRG 1 cut(s) 103
Bsp119I TTCGAA 1 cut(s) 860
Bsp1286I GDGCHC 1 cut(s) 823
Bsp13I TCCGGA 2 cut(s) 517, 849
Bsp1407I TGTACA 1 cut(s) 438
Bsp143I GATC 9 cut(s) 21, 40, 226, 288, 330, 558, 754, 828, 883
Bsp19I CCATGG 1 cut(s) 298
BspANI GGCC 5 cut(s) 93, 347, 405, 503, 666
BspEI TCCGGA 2 cut(s) 517, 849
BspFNI CGCG 6 cut(s) 263, 379, 465, 615, 617, 919
BspMI ACCTGC 1 cut(s) 763
BspPI GGATC 2 cut(s) 553, 891
BspT104I TTCGAA 1 cut(s) 860
BspTNI GGTCTC 2 cut(s) 113, 994
BsrBI CCGCTC 1 cut(s) 234
BsrDI GCAATG 1 cut(s) 974
BsrFI RCCGGY 1 cut(s) 496
BsrGI TGTACA 1 cut(s) 438
BssAI RCCGGY 1 cut(s) 496
BssECI CCNNGG 4 cut(s) 298, 406, 727, 814
BssHII GCGCGC 1 cut(s) 613
BssMI GATC 9 cut(s) 21, 40, 226, 288, 330, 558, 754, 828, 883
BssNI GRCGYC 1 cut(s) 183
BssSI CACGAG 3 cut(s) 353, 824, 911
BssT1I CCWWGG 1 cut(s) 298
Bst2BI CACGAG 3 cut(s) 353, 824, 911
Bst2UI CCWGG 2 cut(s) 407, 729
Bst4CI ACNGT 6 cut(s) 309, 326, 389, 703, 722, 742
BstACI GRCGYC 1 cut(s) 183
BstAUI TGTACA 1 cut(s) 438
BstBI TTCGAA 1 cut(s) 860
BstC8I GCNNGC 8 cut(s) 265, 457, 461, 498, 615, 664, 686, 1005
BstDSI CCRYGG 1 cut(s) 298
BstF5I GGATG 2 cut(s) 117, 805
BstFNI CGCG 6 cut(s) 263, 379, 465, 615, 617, 919
BstHHI GCGC 6 cut(s) 263, 379, 465, 615, 617, 919
BstKTI GATC 9 cut(s) 24, 43, 229, 291, 333, 561, 757, 831, 886
BstMAI GTCTC 3 cut(s) 113, 926, 994
BstMBI GATC 9 cut(s) 21, 40, 226, 288, 330, 558, 754, 828, 883
BstMCI CGRYCG 1 cut(s) 333
BstNI CCWGG 2 cut(s) 407, 729
BstPAI GACNNNNGTC 2 cut(s) 105, 368
BstSCI CCNGG 4 cut(s) 405, 727, 814, 888
BstUI CGCG 6 cut(s) 263, 379, 465, 615, 617, 919
BstV1I GCAGC 5 cut(s) 175, 370, 700, 732, 1083
BstX2I RGATCY 1 cut(s) 558
BstYI RGATCY 1 cut(s) 558
BsuRI GGCC 5 cut(s) 93, 347, 405, 503, 666
BtgI CCRYGG 1 cut(s) 298
BtsCI GGATG 2 cut(s) 117, 805
BtsIMutI CAGTG 2 cut(s) 727, 1099
BveI ACCTGC 1 cut(s) 763
Cac8I GCNNGC 8 cut(s) 265, 457, 461, 498, 615, 664, 686, 1005
CfoI GCGC 6 cut(s) 263, 379, 465, 615, 617, 919
Cfr10I RCCGGY 1 cut(s) 496
Cfr13I GGNCC 3 cut(s) 488, 664, 971
CseI GACGC 2 cut(s) 191, 908
Csp6I GTAC 4 cut(s) 15, 327, 439, 845
CviAII CATG 1 cut(s) 299
CviQI GTAC 4 cut(s) 15, 327, 439, 845
DpnI GATC 9 cut(s) 23, 42, 228, 290, 332, 560, 756, 830, 885
DpnII GATC 9 cut(s) 21, 40, 226, 288, 330, 558, 754, 828, 883
DraIII CACNNNGTG 2 cut(s) 722, 892
EaeI YGGCCR 1 cut(s) 501
Eco130I CCWWGG 1 cut(s) 298
Eco147I AGGCCT 2 cut(s) 93, 347
Eco31I GGTCTC 2 cut(s) 113, 994
Eco47I GGWCC 2 cut(s) 488, 971
Eco57I CTGAAG 3 cut(s) 69, 458, 647
Eco88I CYCGRG 1 cut(s) 103
EcoRI GAATTC 1 cut(s) 1034
EcoRII CCWGG 2 cut(s) 405, 727
EcoT14I CCWWGG 1 cut(s) 298
ErhI CCWWGG 1 cut(s) 298
Esp3I CGTCTC 1 cut(s) 926
FaeI CATG 1 cut(s) 302
FaiI YATR 4 cut(s) 39, 300, 305, 759
FaqI GGGAC 1 cut(s) 545
FatI CATG 1 cut(s) 298
FauI CCCGC 4 cut(s) 147, 655, 684, 693
FbaI TGATCA 1 cut(s) 828
FblI GTMKAC 2 cut(s) 99, 432
FokI GGATG 2 cut(s) 104, 812
GlaI GCGC 6 cut(s) 262, 378, 464, 614, 616, 918
GsaI CCCAGC 2 cut(s) 572, 608
GsuI CTGGAG 1 cut(s) 750
HaeIII GGCC 5 cut(s) 93, 347, 405, 503, 666
HapII CCGG 7 cut(s) 497, 518, 816, 850, 889, 980, 995
HgaI GACGC 2 cut(s) 191, 908
HhaI GCGC 6 cut(s) 263, 379, 465, 615, 617, 919
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 1 cut(s) 302
Hin6I GCGC 6 cut(s) 261, 377, 463, 613, 615, 917
HinP1I GCGC 6 cut(s) 261, 377, 463, 613, 615, 917
HincII GTYRAC 2 cut(s) 100, 433
HindII GTYRAC 2 cut(s) 100, 433
HinfI GANTC 6 cut(s) 71, 84, 101, 126, 901, 943
HpaII CCGG 7 cut(s) 497, 518, 816, 850, 889, 980, 995
HphI GGTGA 7 cut(s) 283, 490, 604, 667, 851, 878, 1093
Hpy166II GTNNAC 3 cut(s) 100, 247, 433
Hpy188I TCNGA 5 cut(s) 83, 214, 226, 948, 1033
Hpy188III TCNNGA 9 cut(s) 286, 320, 518, 644, 752, 826, 850, 1039, 1054
Hpy8I GTNNAC 3 cut(s) 100, 247, 433
Hpy99I CGWCG 8 cut(s) 71, 101, 185, 313, 367, 434, 512, 515
HpyAV CCTTC 5 cut(s) 358, 593, 622, 641, 645
HpyCH4III ACNGT 6 cut(s) 309, 326, 389, 703, 722, 742
HpyCH4IV ACGT 3 cut(s) 429, 513, 834
HpyCH4V TGCA 4 cut(s) 117, 163, 772, 1099
HpySE526I ACGT 3 cut(s) 429, 513, 834
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 1 cut(s) 302
HspAI GCGC 6 cut(s) 261, 377, 463, 613, 615, 917
Kpn2I TCCGGA 2 cut(s) 517, 849
KroI GCCGGC 1 cut(s) 496
KroNI GCCGGC 1 cut(s) 498
Ksp22I TGATCA 1 cut(s) 828
Kzo9I GATC 9 cut(s) 21, 40, 226, 288, 330, 558, 754, 828, 883
LmnI GCTCC 2 cut(s) 577, 982
Lsp1109I GCAGC 5 cut(s) 175, 370, 700, 732, 1083
LweI GCATC 1 cut(s) 126
MaeII ACGT 3 cut(s) 429, 513, 834
MaeIII GTNAC 1 cut(s) 722
MalI GATC 9 cut(s) 23, 42, 228, 290, 332, 560, 756, 830, 885
MbiI CCGCTC 1 cut(s) 234
MboI GATC 9 cut(s) 21, 40, 226, 288, 330, 558, 754, 828, 883
MboII GAAGA 3 cut(s) 62, 274, 1042
MfeI CAATTG 1 cut(s) 158
MflI RGATCY 1 cut(s) 558
MhlI GDGCHC 1 cut(s) 823
MluCI AATT 6 cut(s) 62, 158, 537, 1034, 1061, 1084
MlyI GAGTC 3 cut(s) 80, 93, 95
MmeI TCCRAC 3 cut(s) 237, 450, 586
MroI TCCGGA 2 cut(s) 517, 849
MroNI GCCGGC 1 cut(s) 496
MroXI GAANNNNTTC 1 cut(s) 62
MseI TTAA 2 cut(s) 777, 1092
MslI CAYNNNNRTG 2 cut(s) 762, 786
MspA1I CMGCKG 2 cut(s) 258, 459
MspI CCGG 7 cut(s) 497, 518, 816, 850, 889, 980, 995
MspR9I CCNGG 4 cut(s) 407, 729, 816, 890
MunI CAATTG 1 cut(s) 158
MvaI CCWGG 2 cut(s) 407, 729
MvnI CGCG 6 cut(s) 263, 379, 465, 615, 617, 919
NaeI GCCGGC 1 cut(s) 498
NciI CCSGG 2 cut(s) 816, 890
NcoI CCATGG 1 cut(s) 298
NdeII GATC 9 cut(s) 21, 40, 226, 288, 330, 558, 754, 828, 883
NgoMIV GCCGGC 1 cut(s) 496
NlaIII CATG 1 cut(s) 302
NmuCI GTSAC 1 cut(s) 722
NspV TTCGAA 1 cut(s) 860
OliI CACNNNNGTG 1 cut(s) 786
PauI GCGCGC 1 cut(s) 613
PceI AGGCCT 2 cut(s) 93, 347
PcsI WCGNNNNNNNCGW 2 cut(s) 210, 831
PdiI GCCGGC 1 cut(s) 498
PdmI GAANNNNTTC 1 cut(s) 62
PfeI GAWTC 3 cut(s) 126, 901, 943
PflMI CCANNNNNTGG 1 cut(s) 568
Ple19I CGATCG 1 cut(s) 333
PleI GAGTC 3 cut(s) 79, 92, 95
PpsI GAGTC 3 cut(s) 79, 92, 95
PshAI GACNNNNGTC 2 cut(s) 105, 368
Psp1406I AACGTT 1 cut(s) 834
Psp6I CCWGG 2 cut(s) 405, 727
PspFI CCCAGC 2 cut(s) 568, 604
PspGI CCWGG 2 cut(s) 405, 727
PspPI GGNCC 3 cut(s) 488, 664, 971
PsrI GAACNNNNNNTAC 2 cut(s) 854, 886
PsuI RGATCY 1 cut(s) 558
PteI GCGCGC 1 cut(s) 613
PvuI CGATCG 1 cut(s) 333
PvuII CAGCTG 1 cut(s) 459
RsaI GTAC 4 cut(s) 16, 328, 440, 846
RsaNI GTAC 4 cut(s) 15, 327, 439, 845
RseI CAYNNNNRTG 2 cut(s) 762, 786
SalI GTCGAC 2 cut(s) 98, 431
SaqAI TTAA 2 cut(s) 777, 1092
Sau3AI GATC 9 cut(s) 21, 40, 226, 288, 330, 558, 754, 828, 883
Sau96I GGNCC 3 cut(s) 488, 664, 971
SchI GAGTC 3 cut(s) 80, 93, 95
ScrFI CCNGG 4 cut(s) 407, 729, 816, 890
SduI GDGCHC 1 cut(s) 823
SfaNI GCATC 1 cut(s) 126
SfuI TTCGAA 1 cut(s) 860
SinI GGWCC 2 cut(s) 488, 971
SmiMI CAYNNNNRTG 2 cut(s) 762, 786
Sse9I AATT 6 cut(s) 62, 158, 537, 1034, 1061, 1084
SseBI AGGCCT 2 cut(s) 93, 347
StuI AGGCCT 2 cut(s) 93, 347
StyD4I CCNGG 4 cut(s) 405, 727, 814, 888
StyI CCWWGG 1 cut(s) 298
TaaI ACNGT 6 cut(s) 309, 326, 389, 703, 722, 742
TaiI ACGT 3 cut(s) 432, 516, 837
TaqI TCGA 7 cut(s) 43, 66, 99, 204, 321, 432, 860
TaqII GACCGA 1 cut(s) 476
TasI AATT 6 cut(s) 62, 158, 537, 1034, 1061, 1084
TatI WGTACW 2 cut(s) 438, 844
TauI GCSGC 7 cut(s) 8, 234, 261, 270, 338, 455, 503
TfiI GAWTC 3 cut(s) 126, 901, 943
Tru1I TTAA 2 cut(s) 777, 1092
Tru9I TTAA 2 cut(s) 777, 1092
TscAI CASTG 1 cut(s) 727
TseFI GTSAC 1 cut(s) 722
TseI GCWGC 5 cut(s) 163, 358, 688, 745, 1096
Tsp45I GTSAC 1 cut(s) 722
TspDTI ATGAA 1 cut(s) 650
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 1 cut(s) 727
Van91I CCANNNNNTGG 1 cut(s) 568
VpaK11BI GGWCC 2 cut(s) 488, 971
XapI RAATTY 1 cut(s) 1034
XcmI CCANNNNNNNNNTGG 1 cut(s) 701
XmiI GTMKAC 2 cut(s) 99, 432
XmnI GAANNNNTTC 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.