MD16G1020100.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001116
Physical Location & Seq
Reverse (-)
1443621 .. 1447293
3673 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1020100.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGGCGGCGACCACCGTGATTGGCGAACACGACTACGACGGAGCTCAAGAAATCAAGGATTTCGACAACACAAAACTCGGAGTCAAAGGCCTCGTTGACTCTGGCCTCACCTCCATCCCCCGCTTCTTCATCCACCCACCCGAAACTCTACCCGATACAAAATCCGATTCCCAACAAACACCGATCCCCACCATCGACCTCGCCGGCCTGCACACCCATCACCGTTCATCCATCGTTGACCAAATCAACCAGGCGTGTCGAAAGTTCGGTTTCTTCCAGGTCACAAATCACGGAATCCCGTTGGCAGCTCTGGACCGTACGATCTCAGCCATCAAGGCCTTTCACGAGCAGCCGGCGGAGGTCAAAACACGGGTCTACCGTAGGGATACGGTGACGGGCGTAAATTATGCCTCCAACTTCGACCTCTACAGCTCAAAAGCCGCCAGCTGGAGGGACACGCTAGCAGTTAGGTTGGGCCCCACGCCGGCGAATCCTCTAGAGGTGCCTGAGATCTGTCGGGATGCGGTTGCTGAGTGGGATCAGGAGAGCCAACGGCTGGGAGGCGTGTTGATGGAGCTGGTGAGCGAAGGACTGGGATTGAATGCGTGGAGATTGAGGGAGATGACGTGTCTAGAAGGGAGGATGATGGTGGGCCACTACTACCCCTACTGCCCGCAGCCTGATCTGACGGTGGGGATTGCGTCTCATTCTGACCCGGGAGTTTTGACCGTGCTGCTTCAGGACCAAACGGGTGGATTGCAGGTTAAATACGGTGAGGGTTGGCTGGATGTGAAGCCGGTCCCCGGTGCTCTTGTTATCAACGTCGGGGATTTATTTCAGATTATGTCCAACGGAGAATACAAAAGCGTAGATCATCGGGTGTTAGCTAACCCAAAACACGAAGCACGTGTGGCAATTGCAGTGTTCTTCAGGCCAAGTAATACAGAGGATACGTATGGTCCACTATCGGAGATGGTCTCGCCGGAGAAACCGGCACTTTACCAGCAATTTACGTTGTCAGAATTGCTGCAAAGGTTCTTCAAGAAAGGATTGGATGGCAAAGCTTTGGTCAATCACTTTAGGCTTTGGCCAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

366

Amino Acids

40.5

Weight (kDa)

5.84

Isoelectric Point (pI)

30.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 62 - 159 1.9e-20 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 215 - 312 5e-33 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 751
AccB1I GGYRCC 1 cut(s) 502
AccB7I CCANNNNNTGG 1 cut(s) 556
AccI GTMKAC 1 cut(s) 375
AciI CCGC 6 cut(s) 5, 121, 356, 441, 524, 674
AclWI GGATC 2 cut(s) 178, 546
AcoI YGGCCR 1 cut(s) 1088
AcuI CTGAAG 2 cut(s) 722, 913
AcvI CACGTG 1 cut(s) 908
AfaI GTAC 1 cut(s) 319
AfiI CCNNNNNNNGG 5 cut(s) 447, 450, 484, 556, 803
AflIII ACRYGT 2 cut(s) 626, 907
AgsI TTSAA 2 cut(s) 601, 1042
AjiI CACGTC 1 cut(s) 627
AjnI CCWGG 2 cut(s) 249, 276
AluBI AGCT 7 cut(s) 44, 308, 432, 447, 577, 887, 1064
AluI AGCT 7 cut(s) 44, 308, 432, 447, 577, 887, 1064
Alw21I GWGCWC 2 cut(s) 46, 811
Alw26I GTCTC 2 cut(s) 708, 982
AlwI GGATC 2 cut(s) 178, 546
Ama87I CYCGRG 1 cut(s) 715
AoxI GGCC 8 cut(s) 88, 103, 205, 336, 475, 652, 932, 1088
ApaI GGGCCC 1 cut(s) 479
ApeKI GCWGC 5 cut(s) 305, 349, 676, 733, 1027
ArsI GACNNNNNNTTYG 4 cut(s) 357, 389, 1053, 1085
AspS9I GGNCC 7 cut(s) 313, 475, 476, 652, 742, 799, 959
AsuC2I CCSGG 3 cut(s) 716, 717, 804
AsuHPI GGTGA 5 cut(s) 100, 212, 403, 592, 785
AsuNHI GCTAGC 1 cut(s) 460
AvaI CYCGRG 1 cut(s) 715
AvaII GGWCC 4 cut(s) 313, 742, 799, 959
BaeGI GKGCMC 1 cut(s) 479
BalI TGGCCA 1 cut(s) 1090
BanI GGYRCC 1 cut(s) 502
BanII GRGCYC 2 cut(s) 46, 479
BauI CACGAG 1 cut(s) 344
BbrPI CACGTG 1 cut(s) 908
Bbv12I GWGCWC 2 cut(s) 46, 811
BbvI GCAGC 5 cut(s) 317, 361, 688, 720, 1014
BccI CCATC 9 cut(s) 122, 200, 225, 239, 338, 565, 640, 967, 1049
BceAI ACGGC 1 cut(s) 569
BciT130I CCWGG 2 cut(s) 251, 278
BciVI GTATCC 2 cut(s) 379, 943
BcnI CCSGG 3 cut(s) 716, 717, 804
BcoDI GTCTC 2 cut(s) 708, 982
BfaI CTAG 3 cut(s) 461, 497, 632
BfmI CTRYAG 1 cut(s) 427
BfuAI ACCTGC 1 cut(s) 751
BfuI GTATCC 2 cut(s) 379, 943
BglI GCCNNNNNGGC 1 cut(s) 335
BglII AGATCT 1 cut(s) 510
BisI GCNGC 7 cut(s) 6, 306, 350, 441, 677, 734, 1028
BlsI GCNGC 7 cut(s) 7, 307, 351, 442, 678, 735, 1029
Bme1390I CCNGG 5 cut(s) 251, 278, 716, 717, 804
Bme18I GGWCC 4 cut(s) 313, 742, 799, 959
BmeT110I CYCGRG 1 cut(s) 715
BmgBI CACGTC 1 cut(s) 627
BmgT120I GGNCC 7 cut(s) 313, 475, 476, 652, 742, 799, 959
BmiI GGNNCC 4 cut(s) 477, 478, 504, 801
BmrFI CCNGG 5 cut(s) 251, 278, 716, 717, 804
BmrI ACTGGG 1 cut(s) 602
BmsI GCATC 1 cut(s) 511
BmtI GCTAGC 1 cut(s) 464
BmuI ACTGGG 1 cut(s) 602
BplI GAGNNNNNCTC 2 cut(s) 962, 994
BpmI CTGGAG 1 cut(s) 469
BpuEI CTTGAG 1 cut(s) 30
BpuMI CCSGG 3 cut(s) 716, 717, 804
BsaAI YACGTR 2 cut(s) 908, 954
BsaI GGTCTC 1 cut(s) 982
BsaJI CCNNGG 2 cut(s) 715, 802
BsaXI ACNNNNNCTCC 2 cut(s) 611, 641
Bsc4I CCNNNNNNNGG 5 cut(s) 447, 450, 484, 556, 803
Bse118I RCCGGY 5 cut(s) 203, 352, 484, 796, 991
Bse1I ACTGG 1 cut(s) 597
BseBI CCWGG 2 cut(s) 251, 278
BseDI CCNNGG 2 cut(s) 715, 802
BseGI GGATG 7 cut(s) 114, 129, 227, 526, 648, 793, 1060
BseLI CCNNNNNNNGG 5 cut(s) 447, 450, 484, 556, 803
BseMII CTCAG 3 cut(s) 339, 498, 522
BseNI ACTGG 1 cut(s) 597
BseSI GKGCMC 1 cut(s) 479
BseXI GCAGC 5 cut(s) 317, 361, 688, 720, 1014
BseYI CCCAGC 1 cut(s) 556
BsgI GTGCAG 1 cut(s) 194
BshFI GGCC 8 cut(s) 90, 105, 207, 338, 477, 654, 934, 1090
BshNI GGYRCC 1 cut(s) 502
BsiHKAI GWGCWC 2 cut(s) 46, 811
BsiHKCI CYCGRG 1 cut(s) 715
BsiSI CCGG 8 cut(s) 204, 353, 485, 716, 797, 804, 983, 992
BsiWI CGTACG 1 cut(s) 317
BslFI GGGAC 2 cut(s) 467, 785
BslI CCNNNNNNNGG 5 cut(s) 447, 450, 484, 556, 803
BsmAI GTCTC 2 cut(s) 708, 982
BsmBI CGTCTC 1 cut(s) 708
BsmFI GGGAC 2 cut(s) 467, 785
BsmI GAATGC 1 cut(s) 607
BsnI GGCC 8 cut(s) 90, 105, 207, 338, 477, 654, 934, 1090
Bso31I GGTCTC 1 cut(s) 982
BsoBI CYCGRG 1 cut(s) 715
Bsp120I GGGCCC 1 cut(s) 475
Bsp1286I GDGCHC 3 cut(s) 46, 479, 811
Bsp143I GATC 6 cut(s) 183, 321, 510, 538, 682, 871
BspACI CCGC 6 cut(s) 5, 121, 356, 441, 524, 674
BspANI GGCC 8 cut(s) 90, 105, 207, 338, 477, 654, 934, 1090
BspCNI CTCAG 3 cut(s) 338, 499, 523
BspLI GGNNCC 4 cut(s) 477, 478, 504, 801
BspMI ACCTGC 1 cut(s) 751
BspOI GCTAGC 1 cut(s) 464
BspPI GGATC 2 cut(s) 178, 546
BspT107I GGYRCC 1 cut(s) 502
BspTNI GGTCTC 1 cut(s) 982
BsrFI RCCGGY 5 cut(s) 203, 352, 484, 796, 991
BsrI ACTGG 1 cut(s) 597
BssAI RCCGGY 5 cut(s) 203, 352, 484, 796, 991
BssECI CCNNGG 2 cut(s) 715, 802
BssMI GATC 6 cut(s) 183, 321, 510, 538, 682, 871
BssSI CACGAG 1 cut(s) 344
Bst2BI CACGAG 1 cut(s) 344
Bst2UI CCWGG 2 cut(s) 251, 278
Bst4CI ACNGT 8 cut(s) 16, 224, 317, 380, 391, 691, 730, 773
BstBAI YACGTR 2 cut(s) 908, 954
BstC8I GCNNGC 7 cut(s) 205, 209, 354, 445, 462, 486, 674
BstDEI CTNAG 3 cut(s) 325, 507, 531
BstF5I GGATG 7 cut(s) 114, 129, 227, 526, 648, 793, 1060
BstKTI GATC 6 cut(s) 186, 324, 513, 541, 685, 874
BstMAI GTCTC 2 cut(s) 708, 982
BstMBI GATC 6 cut(s) 183, 321, 510, 538, 682, 871
BstMWI GCNNNNNNNGC 2 cut(s) 335, 911
BstNI CCWGG 2 cut(s) 251, 278
BstSCI CCNGG 5 cut(s) 249, 276, 714, 715, 802
BstSFI CTRYAG 1 cut(s) 427
BstSLI GKGCMC 1 cut(s) 479
BstSNI TACGTA 1 cut(s) 954
BstV1I GCAGC 5 cut(s) 317, 361, 688, 720, 1014
BstX2I RGATCY 1 cut(s) 510
BstXI CCANNNNNNTGG 1 cut(s) 752
BstYI RGATCY 1 cut(s) 510
BsuI GTATCC 2 cut(s) 379, 943
BsuRI GGCC 8 cut(s) 90, 105, 207, 338, 477, 654, 934, 1090
BtrI CACGTC 1 cut(s) 627
BtsCI GGATG 7 cut(s) 114, 129, 227, 526, 648, 793, 1060
BtsI GCAGTG 1 cut(s) 927
BtsIMutI CAGTG 1 cut(s) 927
BveI ACCTGC 1 cut(s) 751
Cac8I GCNNGC 7 cut(s) 205, 209, 354, 445, 462, 486, 674
Cfr10I RCCGGY 5 cut(s) 203, 352, 484, 796, 991
Cfr13I GGNCC 7 cut(s) 313, 475, 476, 652, 742, 799, 959
Cfr9I CCCGGG 1 cut(s) 715
CseI GACGC 1 cut(s) 690
Csp6I GTAC 1 cut(s) 318
CspCI CAANNNNNGTGG 1 cut(s) 36
CviQI GTAC 1 cut(s) 318
DdeI CTNAG 3 cut(s) 325, 507, 531
DpnI GATC 6 cut(s) 185, 323, 512, 540, 684, 873
DpnII GATC 6 cut(s) 183, 321, 510, 538, 682, 871
EaeI YGGCCR 1 cut(s) 1088
EciI GGCGGA 1 cut(s) 371
Ecl136II GAGCTC 1 cut(s) 44
Eco105I TACGTA 1 cut(s) 954
Eco147I AGGCCT 2 cut(s) 90, 338
Eco24I GRGCYC 2 cut(s) 46, 479
Eco31I GGTCTC 1 cut(s) 982
Eco47I GGWCC 4 cut(s) 313, 742, 799, 959
Eco53kI GAGCTC 1 cut(s) 44
Eco57I CTGAAG 2 cut(s) 722, 913
Eco72I CACGTG 1 cut(s) 908
Eco88I CYCGRG 1 cut(s) 715
EcoICRI GAGCTC 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 476
EcoRII CCWGG 2 cut(s) 249, 276
EcoT38I GRGCYC 2 cut(s) 46, 479
Esp3I CGTCTC 1 cut(s) 708
FaiI YATR 3 cut(s) 408, 845, 957
FaqI GGGAC 2 cut(s) 467, 785
FauI CCCGC 2 cut(s) 128, 681
FblI GTMKAC 1 cut(s) 375
Fnu4HI GCNGC 7 cut(s) 6, 306, 350, 441, 677, 734, 1028
FokI GGATG 7 cut(s) 101, 116, 214, 533, 655, 800, 1067
FriOI GRGCYC 2 cut(s) 46, 479
Fsp4HI GCNGC 7 cut(s) 6, 306, 350, 441, 677, 734, 1028
FspBI CTAG 3 cut(s) 461, 497, 632
GluI GCNGC 7 cut(s) 6, 306, 350, 441, 677, 734, 1028
GsaI CCCAGC 1 cut(s) 560
GsuI CTGGAG 1 cut(s) 469
HaeIII GGCC 8 cut(s) 90, 105, 207, 338, 477, 654, 934, 1090
HapII CCGG 8 cut(s) 204, 353, 485, 716, 797, 804, 983, 992
HgaI GACGC 1 cut(s) 690
HincII GTYRAC 2 cut(s) 97, 238
HindII GTYRAC 2 cut(s) 97, 238
HindIII AAGCTT 1 cut(s) 1062
HinfI GANTC 5 cut(s) 81, 98, 167, 294, 490
HpaII CCGG 8 cut(s) 204, 353, 485, 716, 797, 804, 983, 992
HphI GGTGA 5 cut(s) 100, 212, 403, 592, 785
Hpy166II GTNNAC 4 cut(s) 97, 238, 376, 962
Hpy188I TCNGA 7 cut(s) 80, 166, 687, 712, 840, 970, 1021
Hpy188III TCNNGA 9 cut(s) 47, 311, 344, 497, 518, 542, 632, 740, 1042
Hpy8I GTNNAC 4 cut(s) 97, 238, 376, 962
Hpy99I CGWCG 2 cut(s) 41, 827
HpyAV CCTTC 2 cut(s) 581, 629
HpyCH4III ACNGT 8 cut(s) 16, 224, 317, 380, 391, 691, 730, 773
HpyCH4IV ACGT 5 cut(s) 626, 822, 907, 953, 1013
HpyCH4V TGCA 4 cut(s) 211, 760, 920, 1030
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 911
HpyF3I CTNAG 3 cut(s) 325, 507, 531
HpySE526I ACGT 5 cut(s) 626, 822, 907, 953, 1013
KroI GCCGGC 3 cut(s) 203, 352, 484
KroNI GCCGGC 3 cut(s) 205, 354, 486
Kzo9I GATC 6 cut(s) 183, 321, 510, 538, 682, 871
LmnI GCTCC 2 cut(s) 41, 574
Lsp1109I GCAGC 5 cut(s) 317, 361, 688, 720, 1014
LweI GCATC 1 cut(s) 511
MaeI CTAG 3 cut(s) 461, 497, 632
MaeII ACGT 5 cut(s) 626, 822, 907, 953, 1013
MaeIII GTNAC 2 cut(s) 280, 391
MalI GATC 6 cut(s) 185, 323, 512, 540, 684, 873
MboI GATC 6 cut(s) 183, 321, 510, 538, 682, 871
MboII GAAGA 4 cut(s) 118, 265, 919, 1030
MfeI CAATTG 1 cut(s) 915
MflI RGATCY 1 cut(s) 510
MhlI GDGCHC 3 cut(s) 46, 479, 811
MlsI TGGCCA 1 cut(s) 1090
MluCI AATT 4 cut(s) 403, 915, 1007, 1022
MluNI TGGCCA 1 cut(s) 1090
MlyI GAGTC 2 cut(s) 90, 92
MmeI TCCRAC 2 cut(s) 438, 873
Mox20I TGGCCA 1 cut(s) 1090
MreI CGCCGGCG 1 cut(s) 484
MroNI GCCGGC 3 cut(s) 203, 352, 484
MscI TGGCCA 1 cut(s) 1090
MseI TTAA 1 cut(s) 765
Msp20I TGGCCA 1 cut(s) 1090
MspA1I CMGCKG 1 cut(s) 447
MspI CCGG 8 cut(s) 204, 353, 485, 716, 797, 804, 983, 992
MspR9I CCNGG 5 cut(s) 251, 278, 716, 717, 804
MunI CAATTG 1 cut(s) 915
Mva1269I GAATGC 1 cut(s) 607
MvaI CCWGG 2 cut(s) 251, 278
MwoI GCNNNNNNNGC 2 cut(s) 335, 911
NaeI GCCGGC 3 cut(s) 205, 354, 486
NciI CCSGG 3 cut(s) 716, 717, 804
NdeII GATC 6 cut(s) 183, 321, 510, 538, 682, 871
NgoMIV GCCGGC 3 cut(s) 203, 352, 484
NheI GCTAGC 1 cut(s) 460
NlaIV GGNNCC 4 cut(s) 477, 478, 504, 801
NmuCI GTSAC 2 cut(s) 280, 391
PceI AGGCCT 2 cut(s) 90, 338
PcsI WCGNNNNNNNCGW 1 cut(s) 376
PctI GAATGC 1 cut(s) 607
PdiI GCCGGC 3 cut(s) 205, 354, 486
PfeI GAWTC 3 cut(s) 167, 294, 490
Pfl23II CGTACG 1 cut(s) 317
PflMI CCANNNNNTGG 1 cut(s) 556
PkrI GCNGC 7 cut(s) 7, 307, 351, 442, 678, 735, 1029
PleI GAGTC 2 cut(s) 89, 92
PmaCI CACGTG 1 cut(s) 908
PmlI CACGTG 1 cut(s) 908
PpsI GAGTC 2 cut(s) 89, 92
Ppu21I YACGTR 2 cut(s) 908, 954
Psp124BI GAGCTC 1 cut(s) 46
Psp6I CCWGG 2 cut(s) 249, 276
PspCI CACGTG 1 cut(s) 908
PspFI CCCAGC 1 cut(s) 556
PspGI CCWGG 2 cut(s) 249, 276
PspLI CGTACG 1 cut(s) 317
PspN4I GGNNCC 4 cut(s) 477, 478, 504, 801
PspOMI GGGCCC 1 cut(s) 475
PspPI GGNCC 7 cut(s) 313, 475, 476, 652, 742, 799, 959
PsuI RGATCY 1 cut(s) 510
PvuII CAGCTG 1 cut(s) 447
RsaI GTAC 1 cut(s) 319
RsaNI GTAC 1 cut(s) 318
SacI GAGCTC 1 cut(s) 46
SaqAI TTAA 1 cut(s) 765
SatI GCNGC 7 cut(s) 6, 306, 350, 441, 677, 734, 1028
Sau3AI GATC 6 cut(s) 183, 321, 510, 538, 682, 871
Sau96I GGNCC 7 cut(s) 313, 475, 476, 652, 742, 799, 959
SchI GAGTC 2 cut(s) 90, 92
ScrFI CCNGG 5 cut(s) 251, 278, 716, 717, 804
SduI GDGCHC 3 cut(s) 46, 479, 811
SfaNI GCATC 1 cut(s) 511
SfcI CTRYAG 1 cut(s) 427
SgrAI CRCCGGYG 1 cut(s) 484
SinI GGWCC 4 cut(s) 313, 742, 799, 959
SmaI CCCGGG 1 cut(s) 717
SmlI CTYRAG 1 cut(s) 45
SmoI CTYRAG 1 cut(s) 45
SnaBI TACGTA 1 cut(s) 954
Sse9I AATT 4 cut(s) 403, 915, 1007, 1022
SseBI AGGCCT 2 cut(s) 90, 338
SsiI CCGC 6 cut(s) 5, 121, 356, 441, 524, 674
SspMI CTAG 3 cut(s) 461, 497, 632
SstI GAGCTC 1 cut(s) 46
StuI AGGCCT 2 cut(s) 90, 338
StyD4I CCNGG 5 cut(s) 249, 276, 714, 715, 802
TaaI ACNGT 8 cut(s) 16, 224, 317, 380, 391, 691, 730, 773
TaiI ACGT 5 cut(s) 629, 825, 910, 956, 1016
TaqI TCGA 4 cut(s) 63, 195, 259, 420
TasI AATT 4 cut(s) 403, 915, 1007, 1022
TauI GCSGC 2 cut(s) 8, 443
TfiI GAWTC 3 cut(s) 167, 294, 490
Tru1I TTAA 1 cut(s) 765
Tru9I TTAA 1 cut(s) 765
TscAI CASTG 1 cut(s) 927
TseFI GTSAC 2 cut(s) 280, 391
TseI GCWGC 5 cut(s) 305, 349, 676, 733, 1027
Tsp45I GTSAC 2 cut(s) 280, 391
TspDTI ATGAA 2 cut(s) 118, 216
TspGWI ACGGA 3 cut(s) 54, 306, 867
TspMI CCCGGG 1 cut(s) 715
TspRI CASTG 1 cut(s) 927
Van91I CCANNNNNTGG 1 cut(s) 556
VpaK11BI GGWCC 4 cut(s) 313, 742, 799, 959
XbaI TCTAGA 2 cut(s) 496, 631
XmaI CCCGGG 1 cut(s) 715
XmiI GTMKAC 1 cut(s) 375
XspI CTAG 3 cut(s) 461, 497, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.