Rroxscaffold_5G00383840

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001116
Physical Location & Seq
Reverse (-)
63397657 .. 63398839
1183 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00383840.1

Sequence Viewer

Length: 945 bp
ATGGCGGCAAATAGAACCTTGATCGGCGAACAGGGTTATGATCGAGCTGAAGAAGTGAAGCAATTCGACGAGTCCAAGCTCGGAGTCAAAGGCCTCCAGCTCCAGCCCACGACCCGACCGGACGCCCAGCCAATCCCCACCATCGACCTCTCCGACCTCCACTCTGATCGCCGCTCCGCCATCGTCGACCAAGTCAACGGAGCGTGCCGCAACTTCGGTTTCTTCCAGATCACCAACCACGGCATACCGTCGGTGGTTCTGGACCGTACGATCGCCGCCGTCAAGGCCTTCCACGAGCAGCCTACGGAAGTCAAAGCGCAGTTCTACCGTAGAGAGATGATAGGCCATGGCGTAGCTTATCGATCCAACGTTGACCTGTACACCTCCAAAGCCGCCAGCTGGCGCGACACGCTTCAGGAGCTTCTGGGGCTGGTGAGTGAAGGGCTGGGATTGCCAGCGGAGACGCTGAAGGAGATGACGTGTCTGGGTGGGAGGGCGATGGTGGGCCACTACTACCCGCCCTGCCCGCAACCTGATCTAACGGTTGGGCTTACTTCGCATAGTGATCCGGGAGTCTTGACCGTGCTGCTTCAGGATCACATTGGTGGGTTACAGGTTAAGCACGATGGCGCTTGGGTGGATGTGAAGCCTGTCCCCGGTGCTCTTGTGATCAATGTTGGTGATTTACTCCAGATTATTTCAAATGAGGAATACAAAAGTGTCGATCACCGGGTGTTGGCAAATCCACACCACGAGCCGCGTGTCTCGATGGCCGTGTTTTTGAACCCGAGCAACAGAGAGGGGGTGTTTGGTCCGTTGCCAGAGTTCGTCTCACCGGAGAAGCCCGCTCTGTACAAAGAGTTTAAGTTTTCGGAATTCCTATTGAGTTTCTTCAAGAAAGAATTGAATGGCAAATCTTTGGTAAATTACTATAAGCTGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

314

Amino Acids

34.85

Weight (kDa)

6.13

Isoelectric Point (pI)

38.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 45 - 138 4.6e-19 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 166 - 263 5.2e-34 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 174, 848
AccI GTMKAC 1 cut(s) 186
AccII CGCG 2 cut(s) 405, 760
AclI AACGTT 1 cut(s) 369
AclWI GGATC 3 cut(s) 357, 560, 603
AcoI YGGCCR 1 cut(s) 771
AcsI RAATTY 1 cut(s) 875
AcuI CTGAAG 4 cut(s) 69, 398, 488, 575
AcyI GRCGYC 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 733
AfaI GTAC 3 cut(s) 268, 380, 854
AfiI CCNNNNNNNGG 3 cut(s) 399, 656, 736
AflIII ACRYGT 1 cut(s) 479
AgsI TTSAA 4 cut(s) 702, 784, 895, 907
AjiI CACGTC 1 cut(s) 480
AleI CACNNNNGTG 1 cut(s) 603
AluBI AGCT 7 cut(s) 47, 79, 100, 356, 399, 421, 937
AluI AGCT 7 cut(s) 47, 79, 100, 356, 399, 421, 937
Alw21I GWGCWC 1 cut(s) 664
Alw26I GTCTC 3 cut(s) 455, 769, 835
AlwI GGATC 3 cut(s) 357, 560, 603
Ama87I CYCGRG 1 cut(s) 787
AoxI GGCC 5 cut(s) 91, 285, 343, 505, 771
ApeKI GCWGC 3 cut(s) 298, 586, 937
ApoI RAATTY 1 cut(s) 875
Asp700I GAANNNNTTC 1 cut(s) 62
AspLEI GCGC 3 cut(s) 319, 405, 632
AspS9I GGNCC 3 cut(s) 262, 505, 812
AsuC2I CCSGG 3 cut(s) 570, 657, 731
AsuHPI GGTGA 5 cut(s) 223, 445, 692, 719, 825
AvaI CYCGRG 1 cut(s) 787
AvaII GGWCC 2 cut(s) 262, 812
BauI CACGAG 2 cut(s) 293, 752
Bbv12I GWGCWC 1 cut(s) 664
BbvI GCAGC 3 cut(s) 310, 573, 924
BccI CCATC 5 cut(s) 149, 188, 493, 620, 763
BceAI ACGGC 3 cut(s) 256, 263, 758
BclI TGATCA 1 cut(s) 669
BcnI CCSGG 3 cut(s) 570, 657, 731
BcoDI GTCTC 3 cut(s) 455, 769, 835
BfmI CTRYAG 1 cut(s) 938
BfoI RGCGCY 1 cut(s) 633
BglI GCCNNNNNGGC 1 cut(s) 284
BisI GCNGC 9 cut(s) 6, 172, 208, 276, 299, 393, 587, 758, 938
BlsI GCNGC 9 cut(s) 7, 173, 209, 277, 300, 394, 588, 759, 939
Bme1390I CCNGG 3 cut(s) 570, 657, 731
Bme18I GGWCC 2 cut(s) 262, 812
BmeT110I CYCGRG 1 cut(s) 787
BmgBI CACGTC 1 cut(s) 480
BmgT120I GGNCC 3 cut(s) 262, 505, 812
BmrFI CCNGG 3 cut(s) 570, 657, 731
BplI GAGNNNNNCTC 2 cut(s) 815, 847
BpmI CTGGAG 3 cut(s) 80, 86, 674
BpuMI CCSGG 3 cut(s) 570, 657, 731
Bsa29I ATCGAT 1 cut(s) 361
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 3 cut(s) 238, 346, 655
BsaWI WCCGGW 2 cut(s) 118, 835
BsaXI ACNNNNNCTCC 2 cut(s) 464, 494
Bsc4I CCNNNNNNNGG 3 cut(s) 399, 656, 736
BseCI ATCGAT 1 cut(s) 361
BseDI CCNNGG 3 cut(s) 238, 346, 655
BseGI GGATG 1 cut(s) 646
BseLI CCNNNNNNNGG 3 cut(s) 399, 656, 736
BseXI GCAGC 3 cut(s) 310, 573, 924
BseYI CCCAGC 2 cut(s) 126, 445
Bsh1236I CGCG 2 cut(s) 405, 760
Bsh1285I CGRYCG 2 cut(s) 119, 273
BshFI GGCC 5 cut(s) 93, 287, 345, 507, 773
BshVI ATCGAT 1 cut(s) 361
BsiEI CGRYCG 2 cut(s) 119, 273
BsiHKAI GWGCWC 1 cut(s) 664
BsiHKCI CYCGRG 1 cut(s) 787
BsiSI CCGG 5 cut(s) 119, 569, 657, 730, 836
BsiWI CGTACG 1 cut(s) 266
BslFI GGGAC 1 cut(s) 638
BslI CCNNNNNNNGG 3 cut(s) 399, 656, 736
BsmAI GTCTC 3 cut(s) 455, 769, 835
BsmBI CGTCTC 2 cut(s) 455, 835
BsmFI GGGAC 1 cut(s) 638
BsnI GGCC 5 cut(s) 93, 287, 345, 507, 773
BsoBI CYCGRG 1 cut(s) 787
Bsp1286I GDGCHC 1 cut(s) 664
Bsp1407I TGTACA 2 cut(s) 378, 852
Bsp19I CCATGG 1 cut(s) 346
BspANI GGCC 5 cut(s) 93, 287, 345, 507, 773
BspDI ATCGAT 1 cut(s) 361
BspFNI CGCG 2 cut(s) 405, 760
BspMAI CTGCAG 1 cut(s) 942
BspPI GGATC 3 cut(s) 357, 560, 603
BsrBI CCGCTC 2 cut(s) 174, 848
BsrGI TGTACA 2 cut(s) 378, 852
BssECI CCNNGG 3 cut(s) 238, 346, 655
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 2 cut(s) 293, 752
BssT1I CCWWGG 1 cut(s) 346
Bst2BI CACGAG 2 cut(s) 293, 752
Bst4CI ACNGT 5 cut(s) 249, 266, 329, 544, 583
BstACI GRCGYC 1 cut(s) 123
BstAUI TGTACA 2 cut(s) 378, 852
BstC8I GCNNGC 6 cut(s) 205, 397, 401, 456, 527, 846
BstDSI CCRYGG 2 cut(s) 238, 346
BstF5I GGATG 1 cut(s) 646
BstFNI CGCG 2 cut(s) 405, 760
BstH2I RGCGCY 1 cut(s) 633
BstHHI GCGC 3 cut(s) 319, 405, 632
BstMAI GTCTC 3 cut(s) 455, 769, 835
BstMCI CGRYCG 2 cut(s) 119, 273
BstMWI GCNNNNNNNGC 7 cut(s) 284, 409, 418, 427, 451, 526, 556
BstSCI CCNGG 3 cut(s) 568, 655, 729
BstSFI CTRYAG 1 cut(s) 938
BstUI CGCG 2 cut(s) 405, 760
BstV1I GCAGC 3 cut(s) 310, 573, 924
Bsu15I ATCGAT 1 cut(s) 361
BsuRI GGCC 5 cut(s) 93, 287, 345, 507, 773
BsuTUI ATCGAT 1 cut(s) 361
BtgI CCRYGG 2 cut(s) 238, 346
BtgZI GCGATG 1 cut(s) 512
BtrI CACGTC 1 cut(s) 480
BtsCI GGATG 1 cut(s) 646
Cac8I GCNNGC 6 cut(s) 205, 397, 401, 456, 527, 846
CfoI GCGC 3 cut(s) 319, 405, 632
Cfr13I GGNCC 3 cut(s) 262, 505, 812
ClaI ATCGAT 1 cut(s) 361
CseI GACGC 2 cut(s) 131, 472
Csp6I GTAC 3 cut(s) 267, 379, 853
CviAII CATG 1 cut(s) 347
CviQI GTAC 3 cut(s) 267, 379, 853
DraIII CACNNNGTG 1 cut(s) 733
EaeI YGGCCR 1 cut(s) 771
EciI GGCGGA 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 346
Eco147I AGGCCT 2 cut(s) 93, 287
Eco47I GGWCC 2 cut(s) 262, 812
Eco57I CTGAAG 4 cut(s) 69, 398, 488, 575
Eco88I CYCGRG 1 cut(s) 787
EcoRI GAATTC 1 cut(s) 875
EcoT14I CCWWGG 1 cut(s) 346
ErhI CCWWGG 1 cut(s) 346
Esp3I CGTCTC 2 cut(s) 455, 835
FaeI CATG 1 cut(s) 350
FaiI YATR 5 cut(s) 39, 245, 348, 561, 933
FaqI GGGAC 1 cut(s) 638
FatI CATG 1 cut(s) 346
FauI CCCGC 3 cut(s) 525, 534, 853
FbaI TGATCA 1 cut(s) 669
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 9 cut(s) 6, 172, 208, 276, 299, 393, 587, 758, 938
FokI GGATG 1 cut(s) 653
Fsp4HI GCNGC 9 cut(s) 6, 172, 208, 276, 299, 393, 587, 758, 938
GlaI GCGC 3 cut(s) 318, 404, 631
GluI GCNGC 9 cut(s) 6, 172, 208, 276, 299, 393, 587, 758, 938
GsaI CCCAGC 2 cut(s) 130, 449
GsuI CTGGAG 3 cut(s) 80, 86, 674
HaeII RGCGCY 1 cut(s) 633
HaeIII GGCC 5 cut(s) 93, 287, 345, 507, 773
HapII CCGG 5 cut(s) 119, 569, 657, 730, 836
HgaI GACGC 2 cut(s) 131, 472
HhaI GCGC 3 cut(s) 319, 405, 632
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 1 cut(s) 350
Hin6I GCGC 3 cut(s) 317, 403, 630
HinP1I GCGC 3 cut(s) 317, 403, 630
HincII GTYRAC 3 cut(s) 187, 196, 373
HindII GTYRAC 3 cut(s) 187, 196, 373
HinfI GANTC 3 cut(s) 71, 84, 573
HpaII CCGG 5 cut(s) 119, 569, 657, 730, 836
HphI GGTGA 5 cut(s) 223, 445, 692, 719, 825
Hpy166II GTNNAC 4 cut(s) 187, 196, 373, 381
Hpy188I TCNGA 4 cut(s) 83, 154, 166, 874
Hpy188III TCNNGA 8 cut(s) 226, 260, 416, 577, 593, 691, 766, 895
Hpy8I GTNNAC 4 cut(s) 187, 196, 373, 381
Hpy99I CGWCG 3 cut(s) 71, 188, 253
HpyAV CCTTC 3 cut(s) 298, 434, 463
HpyCH4III ACNGT 5 cut(s) 249, 266, 329, 544, 583
HpyCH4IV ACGT 2 cut(s) 369, 479
HpyCH4V TGCA 1 cut(s) 940
HpyF10VI GCNNNNNNNGC 7 cut(s) 284, 409, 418, 427, 451, 526, 556
HpySE526I ACGT 2 cut(s) 369, 479
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 1 cut(s) 350
HspAI GCGC 3 cut(s) 317, 403, 630
Ksp22I TGATCA 1 cut(s) 669
LmnI GCTCC 4 cut(s) 105, 179, 200, 418
Lsp1109I GCAGC 3 cut(s) 310, 573, 924
MaeII ACGT 2 cut(s) 369, 479
MaeIII GTNAC 1 cut(s) 609
MbiI CCGCTC 2 cut(s) 174, 848
MboII GAAGA 3 cut(s) 62, 214, 883
MhlI GDGCHC 1 cut(s) 664
MluCI AATT 4 cut(s) 62, 875, 902, 925
MlyI GAGTC 3 cut(s) 80, 93, 582
MmeI TCCRAC 2 cut(s) 177, 390
MnlI CCTC 7 cut(s) 104, 158, 167, 394, 486, 700, 793
MroXI GAANNNNTTC 1 cut(s) 62
MseI TTAA 2 cut(s) 618, 864
MslI CAYNNNNRTG 1 cut(s) 603
MspA1I CMGCKG 2 cut(s) 399, 458
MspI CCGG 5 cut(s) 119, 569, 657, 730, 836
MspR9I CCNGG 3 cut(s) 570, 657, 731
MvnI CGCG 2 cut(s) 405, 760
MwoI GCNNNNNNNGC 7 cut(s) 284, 409, 418, 427, 451, 526, 556
NciI CCSGG 3 cut(s) 570, 657, 731
NcoI CCATGG 1 cut(s) 346
NlaIII CATG 1 cut(s) 350
OliI CACNNNNGTG 1 cut(s) 603
PceI AGGCCT 2 cut(s) 93, 287
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PdmI GAANNNNTTC 1 cut(s) 62
Pfl23II CGTACG 1 cut(s) 266
PflFI GACNNNGTC 1 cut(s) 191
PfoI TCCNGGA 1 cut(s) 568
PkrI GCNGC 9 cut(s) 7, 173, 209, 277, 300, 394, 588, 759, 939
Ple19I CGATCG 1 cut(s) 273
PleI GAGTC 3 cut(s) 79, 92, 581
PpsI GAGTC 3 cut(s) 79, 92, 581
Psp1406I AACGTT 1 cut(s) 369
PspFI CCCAGC 2 cut(s) 126, 445
PspLI CGTACG 1 cut(s) 266
PspPI GGNCC 3 cut(s) 262, 505, 812
PstI CTGCAG 1 cut(s) 942
PsyI GACNNNGTC 1 cut(s) 191
PvuI CGATCG 1 cut(s) 273
PvuII CAGCTG 1 cut(s) 399
RsaI GTAC 3 cut(s) 268, 380, 854
RsaNI GTAC 3 cut(s) 267, 379, 853
RseI CAYNNNNRTG 1 cut(s) 603
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 2 cut(s) 618, 864
SatI GCNGC 9 cut(s) 6, 172, 208, 276, 299, 393, 587, 758, 938
Sau96I GGNCC 3 cut(s) 262, 505, 812
SchI GAGTC 3 cut(s) 80, 93, 582
ScrFI CCNGG 3 cut(s) 570, 657, 731
SduI GDGCHC 1 cut(s) 664
SfcI CTRYAG 1 cut(s) 938
SinI GGWCC 2 cut(s) 262, 812
SmiMI CAYNNNNRTG 1 cut(s) 603
Sse9I AATT 4 cut(s) 62, 875, 902, 925
SseBI AGGCCT 2 cut(s) 93, 287
StuI AGGCCT 2 cut(s) 93, 287
StyD4I CCNGG 3 cut(s) 568, 655, 729
StyI CCWWGG 1 cut(s) 346
TaaI ACNGT 5 cut(s) 249, 266, 329, 544, 583
TaiI ACGT 2 cut(s) 372, 482
TaqI TCGA 7 cut(s) 43, 66, 144, 186, 361, 723, 767
TasI AATT 4 cut(s) 62, 875, 902, 925
TatI WGTACW 2 cut(s) 378, 852
TauI GCSGC 6 cut(s) 8, 174, 210, 278, 395, 760
Tru1I TTAA 2 cut(s) 618, 864
Tru9I TTAA 2 cut(s) 618, 864
TseI GCWGC 3 cut(s) 298, 586, 937
TspGWI ACGGA 3 cut(s) 213, 320, 804
Tth111I GACNNNGTC 1 cut(s) 191
VpaK11BI GGWCC 2 cut(s) 262, 812
XapI RAATTY 1 cut(s) 875
XmiI GTMKAC 1 cut(s) 186
XmnI GAANNNNTTC 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.