MD16G1020000.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001116
Physical Location & Seq
Reverse (-)
1440662 .. 1443260
2599 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1020000.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGGCGGCGAACACCACGATCGGCGGTGAACGGGATTACGATAGAGCTGAAGAAGTCAAGAAATTCGACCAATCCAAGTTCGGGGTTAAAGGCCTCGTTGACTCAGGCCTCACCTCCATCCCCCGCTTCTTCGTCCACCCACCCGAATCTCTACCCGACCCCCGACATCATACCAAACCCGATACCCCAATCCCCACCATCGACCTGGCGGATCTTCACACCGATCGCCGCTCCTCCGTCGTTGACCAAATCAGCCGCGCCTGCCGAACGTTCGGGTTCTTCCAGATCACTAATCACGGCATCCCATCGGTAACTCTTGACCGCACGATCGCAGCCGTCAGGGCCTTCCACGAGCAGCCGACGGAGGTGAAAGCGCGGTTCTACCGCAGAGAGATGGGCACGGGCGTATCGTATATCTCCAACGTGGACCTCTACAGCTCAAAAGCCGCCAGCTGGAGGGACACGCTTCAAGTGAGGTTGGGCCCGATTCCCGCTGTTCCTGAAGAAGTACCGGAGATTTGCCGGGAGGATGTAGTTGAGTGGGACCGCGAGATCAAACGGCTGGGGGAGGTGCTGATGGAGCTGGCGAGCGAAGGCCTGGGATTGAACCCGAAGAGGTTGCAGGAGATGACGTGTCTGGAAGGGAGGGTGATGGCGGGCCATTACTACCCGTACTGCCCGCAACCTGATCTGACGGTTGGGCTTACATCTCATTCTGACCCGGGAGTGCTGGCCGTGGTGCTTCAGGACCGCATGGGTGGGCTGCAGGTTAAGTACGGTGACGGTTGGGTGGATGTGAAACCTGTTCCCGGTGCCCTCGTCATCAACGTTGGGGATTTTCTTCAGATTGTGTCCAATGGTGAATACCGAAGTGTAGATCATCGTGTGTTGGCGAACTCATCCCGTGAACCCCGTGTGTCAATCGCTGTGTTTTTCAATGTGAGTAGAAGGGATGACTCATACGGGCCATTGCCGGAGCTGGTCTCCCCAGAGAAACCAGCCCTTTACCGGCAGTTTACGCTTTCAGAGTTCATGCAAAGGTTCTTCAATAAAGGGTTGGATGGCAAAGCTTTGGTAAATCACTTTGAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

365

Amino Acids

40.81

Weight (kDa)

5.97

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 64 - 159 4.3e-20 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 217 - 313 2.3e-29 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 757
AccB1I GGYRCC 1 cut(s) 812
AccBSI CCGCTC 1 cut(s) 231
AccII CGCG 3 cut(s) 258, 376, 549
AclI AACGTT 2 cut(s) 269, 828
AclWI GGATC 1 cut(s) 219
AcoI YGGCCR 1 cut(s) 732
AcsI RAATTY 1 cut(s) 62
AcuI CTGAAG 4 cut(s) 69, 522, 728, 827
AfaI GTAC 3 cut(s) 510, 674, 776
AfiI CCNNNNNNNGG 5 cut(s) 81, 453, 456, 809, 1008
AflIII ACRYGT 1 cut(s) 632
AgsI TTSAA 4 cut(s) 470, 607, 937, 1048
AjiI CACGTC 1 cut(s) 633
AjnI CCWGG 2 cut(s) 204, 597
AluBI AGCT 7 cut(s) 47, 438, 453, 583, 979, 1070, 1090
AluI AGCT 7 cut(s) 47, 438, 453, 583, 979, 1070, 1090
Alw26I GTCTC 1 cut(s) 988
AlwI GGATC 1 cut(s) 219
Ama87I CYCGRG 1 cut(s) 721
AoxI GGCC 8 cut(s) 91, 106, 342, 481, 595, 658, 732, 965
ApaI GGGCCC 1 cut(s) 485
ApeKI GCWGC 3 cut(s) 332, 355, 763
ApoI RAATTY 1 cut(s) 62
AspLEI GCGC 2 cut(s) 260, 376
AspS9I GGNCC 8 cut(s) 342, 427, 481, 482, 544, 658, 748, 965
AsuC2I CCSGG 4 cut(s) 524, 722, 723, 810
AsuHPI GGTGA 6 cut(s) 38, 103, 379, 661, 791, 872
AvaI CYCGRG 1 cut(s) 721
AvaII GGWCC 3 cut(s) 427, 544, 748
BaeGI GKGCMC 3 cut(s) 401, 485, 817
BaeI ACNNNNGTAYC 2 cut(s) 390, 423
BanI GGYRCC 1 cut(s) 812
BanII GRGCYC 1 cut(s) 485
BauI CACGAG 1 cut(s) 350
BbvI GCAGC 3 cut(s) 344, 367, 750
BccI CCATC 7 cut(s) 125, 206, 313, 388, 571, 646, 1055
BceAI ACGGC 4 cut(s) 313, 320, 575, 719
BcgI CGANNNNNNTGC 2 cut(s) 601, 635
BciT130I CCWGG 2 cut(s) 206, 599
BcnI CCSGG 4 cut(s) 524, 722, 723, 810
BcoDI GTCTC 1 cut(s) 988
BfmI CTRYAG 2 cut(s) 433, 764
BfuAI ACCTGC 1 cut(s) 757
BglI GCCNNNNNGGC 1 cut(s) 341
BisI GCNGC 7 cut(s) 6, 229, 256, 333, 356, 447, 764
BlsI GCNGC 7 cut(s) 7, 230, 257, 334, 357, 448, 765
Bme1390I CCNGG 6 cut(s) 206, 524, 599, 722, 723, 810
Bme18I GGWCC 3 cut(s) 427, 544, 748
BmeT110I CYCGRG 1 cut(s) 721
BmgBI CACGTC 1 cut(s) 633
BmgT120I GGNCC 8 cut(s) 342, 427, 481, 482, 544, 658, 748, 965
BmiI GGNNCC 3 cut(s) 483, 545, 814
BmrFI CCNGG 6 cut(s) 206, 524, 599, 722, 723, 810
BmsI GCATC 1 cut(s) 309
BplI GAGNNNNNCTC 2 cut(s) 968, 1000
BpmI CTGGAG 1 cut(s) 475
BpuMI CCSGG 4 cut(s) 524, 722, 723, 810
BsaI GGTCTC 1 cut(s) 988
BsaJI CCNNGG 3 cut(s) 598, 721, 735
BsaWI WCCGGW 1 cut(s) 511
BsaXI ACNNNNNCTCC 2 cut(s) 617, 647
Bsc4I CCNNNNNNNGG 5 cut(s) 81, 453, 456, 809, 1008
Bse118I RCCGGY 1 cut(s) 1008
Bse3DI GCAATG 1 cut(s) 968
BseBI CCWGG 2 cut(s) 206, 599
BseDI CCNNGG 3 cut(s) 598, 721, 735
BseGI GGATG 7 cut(s) 117, 300, 535, 799, 899, 958, 1066
BseLI CCNNNNNNNGG 5 cut(s) 81, 453, 456, 809, 1008
BseMI GCAATG 1 cut(s) 968
BseMII CTCAG 1 cut(s) 117
BseRI GAGGAG 1 cut(s) 223
BseSI GKGCMC 3 cut(s) 401, 485, 817
BseXI GCAGC 3 cut(s) 344, 367, 750
BseYI CCCAGC 1 cut(s) 562
Bsh1236I CGCG 3 cut(s) 258, 376, 549
Bsh1285I CGRYCG 3 cut(s) 21, 226, 330
BshFI GGCC 8 cut(s) 93, 108, 344, 483, 597, 660, 734, 967
BshNI GGYRCC 1 cut(s) 812
BsiEI CGRYCG 3 cut(s) 21, 226, 330
BsiHKCI CYCGRG 1 cut(s) 721
BsiSI CCGG 6 cut(s) 512, 523, 722, 810, 974, 1009
BslFI GGGAC 2 cut(s) 473, 557
BslI CCNNNNNNNGG 5 cut(s) 81, 453, 456, 809, 1008
BsmAI GTCTC 1 cut(s) 988
BsmFI GGGAC 2 cut(s) 473, 557
BsnI GGCC 8 cut(s) 93, 108, 344, 483, 597, 660, 734, 967
Bso31I GGTCTC 1 cut(s) 988
BsoBI CYCGRG 1 cut(s) 721
Bsp120I GGGCCC 1 cut(s) 481
Bsp1286I GDGCHC 3 cut(s) 401, 485, 817
Bsp143I GATC 8 cut(s) 18, 211, 223, 285, 327, 552, 688, 877
BspANI GGCC 8 cut(s) 93, 108, 344, 483, 597, 660, 734, 967
BspCNI CTCAG 1 cut(s) 116
BspFNI CGCG 3 cut(s) 258, 376, 549
BspLI GGNNCC 3 cut(s) 483, 545, 814
BspMAI CTGCAG 1 cut(s) 768
BspMI ACCTGC 1 cut(s) 757
BspPI GGATC 1 cut(s) 219
BspT107I GGYRCC 1 cut(s) 812
BspTNI GGTCTC 1 cut(s) 988
BsrBI CCGCTC 1 cut(s) 231
BsrDI GCAATG 1 cut(s) 968
BsrFI RCCGGY 1 cut(s) 1008
BssAI RCCGGY 1 cut(s) 1008
BssECI CCNNGG 3 cut(s) 598, 721, 735
BssMI GATC 8 cut(s) 18, 211, 223, 285, 327, 552, 688, 877
BssSI CACGAG 1 cut(s) 350
Bst2BI CACGAG 1 cut(s) 350
Bst2UI CCWGG 2 cut(s) 206, 599
Bst4CI ACNGT 3 cut(s) 697, 779, 785
Bst6I CTCTTC 1 cut(s) 608
BstC8I GCNNGC 7 cut(s) 262, 451, 585, 589, 658, 680, 732
BstDEI CTNAG 1 cut(s) 103
BstDSI CCRYGG 1 cut(s) 735
BstF5I GGATG 7 cut(s) 117, 300, 535, 799, 899, 958, 1066
BstFNI CGCG 3 cut(s) 258, 376, 549
BstHHI GCGC 2 cut(s) 260, 376
BstKTI GATC 8 cut(s) 21, 214, 226, 288, 330, 555, 691, 880
BstMAI GTCTC 1 cut(s) 988
BstMBI GATC 8 cut(s) 18, 211, 223, 285, 327, 552, 688, 877
BstMCI CGRYCG 3 cut(s) 21, 226, 330
BstMWI GCNNNNNNNGC 4 cut(s) 261, 341, 580, 1018
BstNI CCWGG 2 cut(s) 206, 599
BstSCI CCNGG 6 cut(s) 204, 522, 597, 720, 721, 808
BstSFI CTRYAG 2 cut(s) 433, 764
BstSLI GKGCMC 3 cut(s) 401, 485, 817
BstUI CGCG 3 cut(s) 258, 376, 549
BstV1I GCAGC 3 cut(s) 344, 367, 750
BstX2I RGATCY 1 cut(s) 211
BstXI CCANNNNNNTGG 1 cut(s) 205
BstYI RGATCY 1 cut(s) 211
BsuRI GGCC 8 cut(s) 93, 108, 344, 483, 597, 660, 734, 967
BtgI CCRYGG 1 cut(s) 735
BtrI CACGTC 1 cut(s) 633
BtsCI GGATG 7 cut(s) 117, 300, 535, 799, 899, 958, 1066
BveI ACCTGC 1 cut(s) 757
Cac8I GCNNGC 7 cut(s) 262, 451, 585, 589, 658, 680, 732
CfoI GCGC 2 cut(s) 260, 376
Cfr10I RCCGGY 1 cut(s) 1008
Cfr13I GGNCC 8 cut(s) 342, 427, 481, 482, 544, 658, 748, 965
Cfr9I CCCGGG 1 cut(s) 721
Csp6I GTAC 3 cut(s) 509, 673, 775
CviAII CATG 2 cut(s) 754, 1033
CviQI GTAC 3 cut(s) 509, 673, 775
DdeI CTNAG 1 cut(s) 103
DpnI GATC 8 cut(s) 20, 213, 225, 287, 329, 554, 690, 879
DpnII GATC 8 cut(s) 18, 211, 223, 285, 327, 552, 688, 877
EaeI YGGCCR 1 cut(s) 732
Eam1104I CTCTTC 1 cut(s) 608
EarI CTCTTC 1 cut(s) 608
EciI GGCGGA 1 cut(s) 224
Eco147I AGGCCT 3 cut(s) 93, 108, 597
Eco24I GRGCYC 1 cut(s) 485
Eco31I GGTCTC 1 cut(s) 988
Eco47I GGWCC 3 cut(s) 427, 544, 748
Eco57I CTGAAG 4 cut(s) 69, 522, 728, 827
Eco88I CYCGRG 1 cut(s) 721
EcoO109I RGGNCCY 1 cut(s) 342
EcoRII CCWGG 2 cut(s) 204, 597
EcoT38I GRGCYC 1 cut(s) 485
FaeI CATG 2 cut(s) 757, 1036
FaiI YATR 5 cut(s) 171, 414, 755, 961, 1034
FaqI GGGAC 2 cut(s) 473, 557
FatI CATG 2 cut(s) 753, 1032
FauI CCCGC 4 cut(s) 131, 499, 649, 687
Fnu4HI GCNGC 7 cut(s) 6, 229, 256, 333, 356, 447, 764
FokI GGATG 7 cut(s) 104, 287, 542, 806, 886, 965, 1073
FriOI GRGCYC 1 cut(s) 485
Fsp4HI GCNGC 7 cut(s) 6, 229, 256, 333, 356, 447, 764
GlaI GCGC 2 cut(s) 259, 375
GluI GCNGC 7 cut(s) 6, 229, 256, 333, 356, 447, 764
GsaI CCCAGC 1 cut(s) 566
GsuI CTGGAG 1 cut(s) 475
HaeIII GGCC 8 cut(s) 93, 108, 344, 483, 597, 660, 734, 967
HapII CCGG 6 cut(s) 512, 523, 722, 810, 974, 1009
HhaI GCGC 2 cut(s) 260, 376
Hin1II CATG 2 cut(s) 757, 1036
Hin6I GCGC 2 cut(s) 258, 374
HinP1I GCGC 2 cut(s) 258, 374
HincII GTYRAC 2 cut(s) 100, 244
HindII GTYRAC 2 cut(s) 100, 244
HindIII AAGCTT 1 cut(s) 1068
HinfI GANTC 4 cut(s) 101, 146, 487, 956
HpaII CCGG 6 cut(s) 512, 523, 722, 810, 974, 1009
HphI GGTGA 6 cut(s) 38, 103, 379, 661, 791, 872
Hpy166II GTNNAC 7 cut(s) 29, 100, 136, 244, 427, 908, 1017
Hpy188I TCNGA 4 cut(s) 693, 718, 846, 1027
Hpy188III TCNNGA 6 cut(s) 58, 283, 317, 500, 638, 746
Hpy8I GTNNAC 7 cut(s) 29, 100, 136, 244, 427, 908, 1017
Hpy99I CGWCG 2 cut(s) 242, 364
HpyAV CCTTC 4 cut(s) 355, 587, 635, 942
HpyCH4III ACNGT 3 cut(s) 697, 779, 785
HpyCH4IV ACGT 4 cut(s) 269, 423, 632, 828
HpyCH4V TGCA 3 cut(s) 622, 766, 1036
HpyF10VI GCNNNNNNNGC 4 cut(s) 261, 341, 580, 1018
HpyF3I CTNAG 1 cut(s) 103
HpySE526I ACGT 4 cut(s) 269, 423, 632, 828
Hsp92II CATG 2 cut(s) 757, 1036
HspAI GCGC 2 cut(s) 258, 374
Kzo9I GATC 8 cut(s) 18, 211, 223, 285, 327, 552, 688, 877
LmnI GCTCC 3 cut(s) 236, 580, 976
Lsp1109I GCAGC 3 cut(s) 344, 367, 750
LweI GCATC 1 cut(s) 309
MaeII ACGT 4 cut(s) 269, 423, 632, 828
MaeIII GTNAC 2 cut(s) 310, 779
MalI GATC 8 cut(s) 20, 213, 225, 287, 329, 554, 690, 879
MbiI CCGCTC 1 cut(s) 231
MboI GATC 8 cut(s) 18, 211, 223, 285, 327, 552, 688, 877
MboII GAAGA 8 cut(s) 62, 121, 206, 271, 515, 625, 833, 1036
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 3 cut(s) 401, 485, 817
MluCI AATT 1 cut(s) 62
MlyI GAGTC 2 cut(s) 95, 950
MmeI TCCRAC 2 cut(s) 444, 1038
MseI TTAA 2 cut(s) 87, 771
MspA1I CMGCKG 2 cut(s) 453, 494
MspI CCGG 6 cut(s) 512, 523, 722, 810, 974, 1009
MspR9I CCNGG 6 cut(s) 206, 524, 599, 722, 723, 810
MvaI CCWGG 2 cut(s) 206, 599
MvnI CGCG 3 cut(s) 258, 376, 549
MwoI GCNNNNNNNGC 4 cut(s) 261, 341, 580, 1018
NciI CCSGG 4 cut(s) 524, 722, 723, 810
NdeII GATC 8 cut(s) 18, 211, 223, 285, 327, 552, 688, 877
NlaIII CATG 2 cut(s) 757, 1036
NlaIV GGNNCC 3 cut(s) 483, 545, 814
NmuCI GTSAC 1 cut(s) 779
PceI AGGCCT 3 cut(s) 93, 108, 597
PcsI WCGNNNNNNNCGW 2 cut(s) 407, 825
PfeI GAWTC 2 cut(s) 146, 487
PkrI GCNGC 7 cut(s) 7, 230, 257, 334, 357, 448, 765
Ple19I CGATCG 3 cut(s) 21, 226, 330
PleI GAGTC 2 cut(s) 95, 950
PpsI GAGTC 2 cut(s) 95, 950
Psp1406I AACGTT 2 cut(s) 269, 828
Psp6I CCWGG 2 cut(s) 204, 597
PspFI CCCAGC 1 cut(s) 562
PspGI CCWGG 2 cut(s) 204, 597
PspN4I GGNNCC 3 cut(s) 483, 545, 814
PspOMI GGGCCC 1 cut(s) 481
PspPI GGNCC 8 cut(s) 342, 427, 481, 482, 544, 658, 748, 965
PstI CTGCAG 1 cut(s) 768
PsuI RGATCY 1 cut(s) 211
PvuI CGATCG 3 cut(s) 21, 226, 330
PvuII CAGCTG 1 cut(s) 453
RsaI GTAC 3 cut(s) 510, 674, 776
RsaNI GTAC 3 cut(s) 509, 673, 775
SaqAI TTAA 2 cut(s) 87, 771
SatI GCNGC 7 cut(s) 6, 229, 256, 333, 356, 447, 764
Sau3AI GATC 8 cut(s) 18, 211, 223, 285, 327, 552, 688, 877
Sau96I GGNCC 8 cut(s) 342, 427, 481, 482, 544, 658, 748, 965
SchI GAGTC 2 cut(s) 95, 950
ScrFI CCNGG 6 cut(s) 206, 524, 599, 722, 723, 810
SduI GDGCHC 3 cut(s) 401, 485, 817
SfaNI GCATC 1 cut(s) 309
SfcI CTRYAG 2 cut(s) 433, 764
SinI GGWCC 3 cut(s) 427, 544, 748
SmaI CCCGGG 1 cut(s) 723
Sse9I AATT 1 cut(s) 62
SseBI AGGCCT 3 cut(s) 93, 108, 597
StuI AGGCCT 3 cut(s) 93, 108, 597
StyD4I CCNGG 6 cut(s) 204, 522, 597, 720, 721, 808
TaaI ACNGT 3 cut(s) 697, 779, 785
TaiI ACGT 4 cut(s) 272, 426, 635, 831
TaqI TCGA 2 cut(s) 66, 201
TasI AATT 1 cut(s) 62
TauI GCSGC 4 cut(s) 8, 231, 258, 449
TfiI GAWTC 2 cut(s) 146, 487
Tru1I TTAA 2 cut(s) 87, 771
Tru9I TTAA 2 cut(s) 87, 771
TseFI GTSAC 1 cut(s) 779
TseI GCWGC 3 cut(s) 332, 355, 763
Tsp45I GTSAC 1 cut(s) 779
TspDTI ATGAA 1 cut(s) 1021
TspGWI ACGGA 2 cut(s) 226, 377
TspMI CCCGGG 1 cut(s) 721
VpaK11BI GGWCC 3 cut(s) 427, 544, 748
XapI RAATTY 1 cut(s) 62
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XmaI CCCGGG 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.