RLG00000005945

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001116
Physical Location & Seq
Forward (+)
2965230 .. 2966406
1177 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005945

Sequence Viewer

Length: 1098 bp
ATGGCGGCAAATGGTACCTTGATCGGCGAACAGGGTTTTGACCGAGCTGACGAAGTGAAGCAATTCGACGACTCCAAGCTCGGAGTCAAAGGCCTCGTCGACTCGGGTCTCTCCTCCATCCCCCGAATCTTTATCCACCCCCTCGAAACCTTACAGCAATTCCAGCCCAAGACCCGACCCGACGCCCAACCAATCCCCACCATCGACCTCTCCAACCTCCACTCCGATCGCCGCTCCGCCATCGTCGACCAAGTCAGCGGCGCGTGCCGCAACTTCGGTTTCTTCCAGATCACCAACCACGGCATACCGTCGGTGGCTCTCGACCGTACGATCGCCGCCGTCAAGGCCTTCCACGAGCAGCCGACGGAGGTCAAGGCGCGGTTCTACCGTAGAGAGATGGGCCTGGGCGTATCGTATTTCTCCAACATCGACCTGTACACGTCCAAAGCCGCCAGCTGGCGCGACACACTTCAGATGAGGCTCAGCCCCGTGCCGGCTGCCGCCGACGACGTTCCGGAGGTTTGTAGGGACGAAATTGCGGCATGGAACCTGGAGATCCAACGGCTGGGAGAGGAGCTGCTGGGGCTGATGAGTGAAGGGCTGGGATTGCCTGCGGAGAAGCTGAAGGAGATGACGTGTCTGGAAGGGAGGGCGATGGTGGGAAACTATTACCCACCCTGCCCGCAACCTGACCTCACCGTAGGGCTTACATCACACAGTGACCCTGGAGTGTTGACCGTGCTGCTGCAAGATCACATCGGTGGGTTGCAGGTTAAGCACGATGATGCTTGGTTGGATGTGAAACCCGTCCCCGGTGCTCTTGTGATCAACGTTGGAGATTTACTCCAGATTATTTCAAACGAGGAATACAAAAGCGTGGATCACCGGGTGACGGCGAATCCACAGCACGAGGCGCGTGTCTCAATAGCCGTGTTTTTGAACCCGAGCAATAGAGAGGGCGTGTTTGGTCCGTTACCGGAGTTGGTCTCGTCGGAGAAGCCAGCTCTCTACAAAGAGTTTAAGTTTACGGAATTCATAATGAGGTTCTTCAAGAAAGAATTGAATGGCAAATCTTTGGTAAATTATTATAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

366

Amino Acids

40.52

Weight (kDa)

5.55

Isoelectric Point (pI)

37.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 65 - 162 9.9e-20 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 218 - 315 6.2e-34 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1089
Acc36I ACCTGC 1 cut(s) 760
Acc65I GGTACC 1 cut(s) 14
AccB1I GGYRCC 1 cut(s) 14
AccB7I CCANNNNNTGG 1 cut(s) 565
AccBSI CCGCTC 1 cut(s) 234
AccI GTMKAC 2 cut(s) 99, 246
AccII CGCG 4 cut(s) 263, 379, 462, 916
AccIII TCCGGA 1 cut(s) 514
AclI AACGTT 1 cut(s) 831
AclWI GGATC 2 cut(s) 550, 888
AcsI RAATTY 1 cut(s) 1031
AcuI CTGAAG 2 cut(s) 455, 644
AcyI GRCGYC 1 cut(s) 183
AdeI CACNNNGTG 2 cut(s) 719, 889
AfaI GTAC 3 cut(s) 16, 328, 437
AfiI CCNNNNNNNGG 5 cut(s) 456, 493, 565, 812, 892
AflIII ACRYGT 2 cut(s) 438, 635
AgsI TTSAA 4 cut(s) 858, 940, 1051, 1063
AjiI CACGTC 2 cut(s) 441, 636
AjnI CCWGG 3 cut(s) 402, 549, 724
AleI CACNNNNGTG 1 cut(s) 759
AluBI AGCT 6 cut(s) 47, 79, 456, 577, 622, 1004
AluI AGCT 6 cut(s) 47, 79, 456, 577, 622, 1004
Alw21I GWGCWC 1 cut(s) 820
Alw26I GTCTC 3 cut(s) 113, 925, 991
AlwI GGATC 2 cut(s) 550, 888
Ama87I CYCGRG 2 cut(s) 103, 943
Aor13HI TCCGGA 1 cut(s) 514
AoxI GGCC 3 cut(s) 91, 345, 400
ApeKI GCWGC 5 cut(s) 358, 497, 577, 742, 745
ApoI RAATTY 1 cut(s) 1031
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
Asp700I GAANNNNTTC 1 cut(s) 62
Asp718I GGTACC 1 cut(s) 14
AspLEI GCGC 4 cut(s) 263, 379, 462, 916
AspS9I GGNCC 2 cut(s) 400, 968
AsuC2I CCSGG 2 cut(s) 813, 887
AsuHPI GGTGA 4 cut(s) 283, 688, 875, 901
AvaI CYCGRG 2 cut(s) 103, 943
AvaII GGWCC 1 cut(s) 968
BanI GGYRCC 1 cut(s) 14
BauI CACGAG 2 cut(s) 353, 908
Bbv12I GWGCWC 1 cut(s) 820
BbvI GCAGC 5 cut(s) 370, 484, 564, 729, 732
BccI CCATC 5 cut(s) 125, 209, 248, 391, 649
BceAI ACGGC 5 cut(s) 316, 323, 578, 909, 914
BciT130I CCWGG 3 cut(s) 404, 551, 726
BclI TGATCA 1 cut(s) 825
BcnI CCSGG 2 cut(s) 813, 887
BcoDI GTCTC 3 cut(s) 113, 925, 991
BfuAI ACCTGC 1 cut(s) 760
BglI GCCNNNNNGGC 1 cut(s) 344
BlpI GCTNAGC 1 cut(s) 482
Bme1390I CCNGG 5 cut(s) 404, 551, 726, 813, 887
Bme18I GGWCC 1 cut(s) 968
BmeT110I CYCGRG 2 cut(s) 103, 943
BmgBI CACGTC 2 cut(s) 441, 636
BmgT120I GGNCC 2 cut(s) 400, 968
BmiI GGNNCC 2 cut(s) 16, 548
BmrFI CCNGG 5 cut(s) 404, 551, 726, 813, 887
BmsI GCATC 1 cut(s) 775
BoxI GACNNNNGTC 2 cut(s) 105, 368
BplI GAGNNNNNCTC 4 cut(s) 828, 860, 971, 1003
BpmI CTGGAG 3 cut(s) 572, 747, 830
Bpu1102I GCTNAGC 1 cut(s) 482
BpuMI CCSGG 2 cut(s) 813, 887
BsaHI GRCGYC 1 cut(s) 183
BsaI GGTCTC 2 cut(s) 113, 991
BsaJI CCNNGG 4 cut(s) 298, 403, 724, 811
BsaWI WCCGGW 2 cut(s) 514, 976
BsaXI ACNNNNNCTCC 4 cut(s) 206, 236, 620, 650
Bsc4I CCNNNNNNNGG 5 cut(s) 456, 493, 565, 812, 892
Bse118I RCCGGY 1 cut(s) 493
BseAI TCCGGA 1 cut(s) 514
BseBI CCWGG 3 cut(s) 404, 551, 726
BseDI CCNNGG 4 cut(s) 298, 403, 724, 811
BseGI GGATG 2 cut(s) 117, 802
BseLI CCNNNNNNNGG 5 cut(s) 456, 493, 565, 812, 892
BseMII CTCAG 1 cut(s) 496
BseRI GAGGAG 2 cut(s) 103, 587
BseXI GCAGC 5 cut(s) 370, 484, 564, 729, 732
BseYI CCCAGC 3 cut(s) 565, 580, 601
Bsh1236I CGCG 4 cut(s) 263, 379, 462, 916
Bsh1285I CGRYCG 3 cut(s) 229, 325, 333
BshFI GGCC 3 cut(s) 93, 347, 402
BshNI GGYRCC 1 cut(s) 14
BsiEI CGRYCG 3 cut(s) 229, 325, 333
BsiHKAI GWGCWC 1 cut(s) 820
BsiHKCI CYCGRG 2 cut(s) 103, 943
BsiSI CCGG 5 cut(s) 494, 515, 813, 886, 977
BsiWI CGTACG 1 cut(s) 326
BslFI GGGAC 2 cut(s) 542, 794
BslI CCNNNNNNNGG 5 cut(s) 456, 493, 565, 812, 892
BsmAI GTCTC 3 cut(s) 113, 925, 991
BsmFI GGGAC 2 cut(s) 542, 794
BsnI GGCC 3 cut(s) 93, 347, 402
Bso31I GGTCTC 2 cut(s) 113, 991
BsoBI CYCGRG 2 cut(s) 103, 943
Bsp1286I GDGCHC 1 cut(s) 820
Bsp13I TCCGGA 1 cut(s) 514
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 8 cut(s) 21, 226, 288, 330, 555, 751, 825, 880
Bsp1720I GCTNAGC 1 cut(s) 482
BspANI GGCC 3 cut(s) 93, 347, 402
BspCNI CTCAG 1 cut(s) 495
BspEI TCCGGA 1 cut(s) 514
BspFNI CGCG 4 cut(s) 263, 379, 462, 916
BspLI GGNNCC 2 cut(s) 16, 548
BspMI ACCTGC 1 cut(s) 760
BspPI GGATC 2 cut(s) 550, 888
BspT107I GGYRCC 1 cut(s) 14
BspTNI GGTCTC 2 cut(s) 113, 991
BsrBI CCGCTC 1 cut(s) 234
BsrFI RCCGGY 1 cut(s) 493
BsrGI TGTACA 1 cut(s) 435
BssAI RCCGGY 1 cut(s) 493
BssECI CCNNGG 4 cut(s) 298, 403, 724, 811
BssMI GATC 8 cut(s) 21, 226, 288, 330, 555, 751, 825, 880
BssNI GRCGYC 1 cut(s) 183
BssSI CACGAG 2 cut(s) 353, 908
Bst2BI CACGAG 2 cut(s) 353, 908
Bst2UI CCWGG 3 cut(s) 404, 551, 726
Bst4CI ACNGT 6 cut(s) 309, 326, 389, 700, 719, 739
BstACI GRCGYC 1 cut(s) 183
BstAUI TGTACA 1 cut(s) 435
BstC8I GCNNGC 7 cut(s) 265, 454, 458, 495, 612, 683, 1002
BstDEI CTNAG 1 cut(s) 482
BstDSI CCRYGG 1 cut(s) 298
BstF5I GGATG 2 cut(s) 117, 802
BstFNI CGCG 4 cut(s) 263, 379, 462, 916
BstHHI GCGC 4 cut(s) 263, 379, 462, 916
BstKTI GATC 8 cut(s) 24, 229, 291, 333, 558, 754, 828, 883
BstMAI GTCTC 3 cut(s) 113, 925, 991
BstMBI GATC 8 cut(s) 21, 226, 288, 330, 555, 751, 825, 880
BstMCI CGRYCG 3 cut(s) 229, 325, 333
BstMWI GCNNNNNNNGC 8 cut(s) 163, 264, 267, 344, 583, 607, 775, 913
BstNI CCWGG 3 cut(s) 404, 551, 726
BstPAI GACNNNNGTC 2 cut(s) 105, 368
BstSCI CCNGG 5 cut(s) 402, 549, 724, 811, 885
BstUI CGCG 4 cut(s) 263, 379, 462, 916
BstV1I GCAGC 5 cut(s) 370, 484, 564, 729, 732
BstX2I RGATCY 1 cut(s) 555
BstYI RGATCY 1 cut(s) 555
BsuRI GGCC 3 cut(s) 93, 347, 402
BtgI CCRYGG 1 cut(s) 298
BtgZI GCGATG 1 cut(s) 668
BtrI CACGTC 2 cut(s) 441, 636
BtsCI GGATG 2 cut(s) 117, 802
BtsIMutI CAGTG 1 cut(s) 724
BveI ACCTGC 1 cut(s) 760
Cac8I GCNNGC 7 cut(s) 265, 454, 458, 495, 612, 683, 1002
CfoI GCGC 4 cut(s) 263, 379, 462, 916
Cfr10I RCCGGY 1 cut(s) 493
Cfr13I GGNCC 2 cut(s) 400, 968
CseI GACGC 1 cut(s) 191
Csp6I GTAC 3 cut(s) 15, 327, 436
CviAII CATG 1 cut(s) 543
CviQI GTAC 3 cut(s) 15, 327, 436
DdeI CTNAG 1 cut(s) 482
DpnI GATC 8 cut(s) 23, 228, 290, 332, 557, 753, 827, 882
DpnII GATC 8 cut(s) 21, 226, 288, 330, 555, 751, 825, 880
DraIII CACNNNGTG 2 cut(s) 719, 889
EciI GGCGGA 1 cut(s) 226
Eco147I AGGCCT 2 cut(s) 93, 347
Eco31I GGTCTC 2 cut(s) 113, 991
Eco47I GGWCC 1 cut(s) 968
Eco57I CTGAAG 2 cut(s) 455, 644
Eco88I CYCGRG 2 cut(s) 103, 943
EcoRI GAATTC 1 cut(s) 1031
EcoRII CCWGG 3 cut(s) 402, 549, 724
FaeI CATG 1 cut(s) 546
FaiI YATR 4 cut(s) 305, 544, 1037, 1089
FaqI GGGAC 2 cut(s) 542, 794
FatI CATG 1 cut(s) 542
FauI CCCGC 1 cut(s) 690
FbaI TGATCA 1 cut(s) 825
FblI GTMKAC 2 cut(s) 99, 246
FokI GGATG 2 cut(s) 104, 809
GlaI GCGC 4 cut(s) 262, 378, 461, 915
GsaI CCCAGC 3 cut(s) 569, 584, 605
GsuI CTGGAG 3 cut(s) 572, 747, 830
HaeIII GGCC 3 cut(s) 93, 347, 402
HapII CCGG 5 cut(s) 494, 515, 813, 886, 977
HgaI GACGC 1 cut(s) 191
HhaI GCGC 4 cut(s) 263, 379, 462, 916
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 1 cut(s) 546
Hin6I GCGC 4 cut(s) 261, 377, 460, 914
HinP1I GCGC 4 cut(s) 261, 377, 460, 914
HincII GTYRAC 3 cut(s) 100, 247, 735
HindII GTYRAC 3 cut(s) 100, 247, 735
HinfI GANTC 5 cut(s) 71, 84, 101, 126, 898
HpaII CCGG 5 cut(s) 494, 515, 813, 886, 977
HphI GGTGA 4 cut(s) 283, 688, 875, 901
Hpy166II GTNNAC 5 cut(s) 100, 247, 438, 735, 1026
Hpy188I TCNGA 4 cut(s) 83, 226, 474, 994
Hpy188III TCNNGA 6 cut(s) 286, 320, 515, 641, 847, 1051
Hpy8I GTNNAC 5 cut(s) 100, 247, 438, 735, 1026
Hpy99I CGWCG 9 cut(s) 71, 101, 185, 248, 313, 367, 509, 512, 994
HpyAV CCTTC 4 cut(s) 358, 590, 619, 638
HpyCH4III ACNGT 6 cut(s) 309, 326, 389, 700, 719, 739
HpyCH4IV ACGT 4 cut(s) 440, 510, 635, 831
HpyCH4V TGCA 2 cut(s) 748, 769
HpyF10VI GCNNNNNNNGC 8 cut(s) 163, 264, 267, 344, 583, 607, 775, 913
HpyF3I CTNAG 1 cut(s) 482
HpySE526I ACGT 4 cut(s) 440, 510, 635, 831
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 1 cut(s) 546
HspAI GCGC 4 cut(s) 261, 377, 460, 914
Kpn2I TCCGGA 1 cut(s) 514
KpnI GGTACC 1 cut(s) 18
KroI GCCGGC 1 cut(s) 493
KroNI GCCGGC 1 cut(s) 495
Ksp22I TGATCA 1 cut(s) 825
Kzo9I GATC 8 cut(s) 21, 226, 288, 330, 555, 751, 825, 880
LmnI GCTCC 2 cut(s) 239, 574
Lsp1109I GCAGC 5 cut(s) 370, 484, 564, 729, 732
LweI GCATC 1 cut(s) 775
MaeII ACGT 4 cut(s) 440, 510, 635, 831
MaeIII GTNAC 3 cut(s) 719, 889, 972
MalI GATC 8 cut(s) 23, 228, 290, 332, 557, 753, 827, 882
MbiI CCGCTC 1 cut(s) 234
MboI GATC 8 cut(s) 21, 226, 288, 330, 555, 751, 825, 880
MboII GAAGA 2 cut(s) 274, 1039
MflI RGATCY 1 cut(s) 555
MhlI GDGCHC 1 cut(s) 820
MluCI AATT 6 cut(s) 62, 158, 534, 1031, 1058, 1081
MlyI GAGTC 3 cut(s) 65, 93, 95
MmeI TCCRAC 6 cut(s) 237, 447, 583, 774, 814, 972
MroI TCCGGA 1 cut(s) 514
MroNI GCCGGC 1 cut(s) 493
MroXI GAANNNNTTC 1 cut(s) 62
MseI TTAA 2 cut(s) 774, 1020
MslI CAYNNNNRTG 2 cut(s) 759, 783
MspA1I CMGCKG 2 cut(s) 258, 456
MspI CCGG 5 cut(s) 494, 515, 813, 886, 977
MspR9I CCNGG 5 cut(s) 404, 551, 726, 813, 887
MvaI CCWGG 3 cut(s) 404, 551, 726
MvnI CGCG 4 cut(s) 263, 379, 462, 916
MwoI GCNNNNNNNGC 8 cut(s) 163, 264, 267, 344, 583, 607, 775, 913
NaeI GCCGGC 1 cut(s) 495
NciI CCSGG 2 cut(s) 813, 887
NdeII GATC 8 cut(s) 21, 226, 288, 330, 555, 751, 825, 880
NgoMIV GCCGGC 1 cut(s) 493
NlaIII CATG 1 cut(s) 546
NlaIV GGNNCC 2 cut(s) 16, 548
NmuCI GTSAC 2 cut(s) 719, 889
OliI CACNNNNGTG 1 cut(s) 759
PceI AGGCCT 2 cut(s) 93, 347
PdiI GCCGGC 1 cut(s) 495
PdmI GAANNNNTTC 1 cut(s) 62
PfeI GAWTC 2 cut(s) 126, 898
Pfl23II CGTACG 1 cut(s) 326
PflFI GACNNNGTC 1 cut(s) 251
PflMI CCANNNNNTGG 1 cut(s) 565
Ple19I CGATCG 2 cut(s) 229, 333
PleI GAGTC 3 cut(s) 65, 92, 95
PpsI GAGTC 3 cut(s) 65, 92, 95
PshAI GACNNNNGTC 2 cut(s) 105, 368
PsiI TTATAA 1 cut(s) 1089
Psp1406I AACGTT 1 cut(s) 831
Psp6I CCWGG 3 cut(s) 402, 549, 724
PspFI CCCAGC 3 cut(s) 565, 580, 601
PspGI CCWGG 3 cut(s) 402, 549, 724
PspLI CGTACG 1 cut(s) 326
PspN4I GGNNCC 2 cut(s) 16, 548
PspPI GGNCC 2 cut(s) 400, 968
PsuI RGATCY 1 cut(s) 555
PsyI GACNNNGTC 1 cut(s) 251
PvuI CGATCG 2 cut(s) 229, 333
PvuII CAGCTG 1 cut(s) 456
RsaI GTAC 3 cut(s) 16, 328, 437
RsaNI GTAC 3 cut(s) 15, 327, 436
RseI CAYNNNNRTG 2 cut(s) 759, 783
SalI GTCGAC 2 cut(s) 98, 245
SaqAI TTAA 2 cut(s) 774, 1020
Sau3AI GATC 8 cut(s) 21, 226, 288, 330, 555, 751, 825, 880
Sau96I GGNCC 2 cut(s) 400, 968
SchI GAGTC 3 cut(s) 65, 93, 95
ScrFI CCNGG 5 cut(s) 404, 551, 726, 813, 887
SduI GDGCHC 1 cut(s) 820
SfaNI GCATC 1 cut(s) 775
SinI GGWCC 1 cut(s) 968
SmiMI CAYNNNNRTG 2 cut(s) 759, 783
Sse9I AATT 6 cut(s) 62, 158, 534, 1031, 1058, 1081
SseBI AGGCCT 2 cut(s) 93, 347
StuI AGGCCT 2 cut(s) 93, 347
StyD4I CCNGG 5 cut(s) 402, 549, 724, 811, 885
TaaI ACNGT 6 cut(s) 309, 326, 389, 700, 719, 739
TaiI ACGT 4 cut(s) 443, 513, 638, 834
TaqI TCGA 7 cut(s) 66, 99, 144, 204, 246, 321, 429
TaqII GACCGA 1 cut(s) 57
TasI AATT 6 cut(s) 62, 158, 534, 1031, 1058, 1081
TatI WGTACW 1 cut(s) 435
TauI GCSGC 8 cut(s) 8, 234, 261, 270, 338, 452, 503, 542
TfiI GAWTC 2 cut(s) 126, 898
Tru1I TTAA 2 cut(s) 774, 1020
Tru9I TTAA 2 cut(s) 774, 1020
TscAI CASTG 1 cut(s) 724
TseFI GTSAC 2 cut(s) 719, 889
TseI GCWGC 5 cut(s) 358, 497, 577, 742, 745
Tsp45I GTSAC 2 cut(s) 719, 889
TspDTI ATGAA 1 cut(s) 1024
TspGWI ACGGA 3 cut(s) 380, 960, 1043
TspRI CASTG 1 cut(s) 724
Tth111I GACNNNGTC 1 cut(s) 251
Van91I CCANNNNNTGG 1 cut(s) 565
VpaK11BI GGWCC 1 cut(s) 968
XapI RAATTY 1 cut(s) 1031
XmiI GTMKAC 2 cut(s) 99, 246
XmnI GAANNNNTTC 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.