pycom16g01780

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001116
Physical Location & Seq
Reverse (-)
1092629 .. 1095046
2418 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g01780.2

Sequence Viewer

Length: 870 bp
ATGGAGAACGCCGTGATCGGCGAACTGGATTACGATAGAGCTAAAGAAATCAAGCAATTCGACAGCTCCAAGCTCGGAGTCAAAGGCCTCGTTGACTCTGGCCTCACCTCCATCCCCCGCTTCTTCATCCACCCGCCCGAATCTCTACCCGACCTCCGACACCATACCAAACCCGATATCCCAATCCCCACCATCGACCTCGCGGATCTTCACACCCATCGCCGCTCCTCCATCGTCGACCAAATCAGCCGCGCTTGCCGCACGTTCGGGTTCTTCCAGATCACTAATCACGGCATCCCATCCGTAATCCTGGACCGCACGATCGCAGCCATCAGGGACTTCCACGAGCAGCCGACGGAGGTGAAAGCGCGCTTCTACCGTAGAGAGATGGGCACGGGCGTATCGTATTTCTCCAACCTGGACCTCTACAGCTCAAAAGCCGCCAGCTGGAGGGACACGCTTCAAATGAGGTTGGGCCCGATTCCGCCGGTTCCTGAAGCAGTGCCGGAGATTTGCCGAGAGGATGTTGTTGAGTGGGACCGTGAGATCAAACGGCTGGGAGAGGTGCTTATGGAGTTGGCGAGCGAAGGGCTGGGATTGAATTCTAAGAGGTTGCGGGAGATGACGTGTCTGGAAGGGAGGGCGATGGTGGGGAATTATTACCCATACTGCCCGCAACCTGATCTAACGGTTGGGCTTACATCTCATTCTGACCCAGGAGTGCTGACCGTGCTGCTTCAGGATCACATGGGTGGGCTGCAGGTGAAGTACGGTGAGGGTTGGGTAGATGTGAAACCTGTCCCCGGTGCTCTCGTTATCAACGTTGGGGATTTTCTTCAATTATTTCCAATGGTGAATACCAAAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

290

Amino Acids

32.44

Weight (kDa)

5.86

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 751
Acc36I ACCTGC 1 cut(s) 751
AccBSI CCGCTC 1 cut(s) 225
AccI GTMKAC 1 cut(s) 237
AccII CGCG 3 cut(s) 203, 252, 370
AclI AACGTT 1 cut(s) 822
AclWI GGATC 2 cut(s) 213, 750
AcsI RAATTY 1 cut(s) 601
AcuI CTGAAG 2 cut(s) 516, 722
AfaI GTAC 1 cut(s) 770
AfiI CCNNNNNNNGG 3 cut(s) 447, 450, 803
AflIII ACRYGT 1 cut(s) 626
AgsI TTSAA 3 cut(s) 464, 601, 839
AjiI CACGTC 1 cut(s) 627
AjnI CCWGG 3 cut(s) 309, 417, 715
AleI CACNNNNGTG 1 cut(s) 750
AluBI AGCT 5 cut(s) 41, 66, 73, 432, 447
AluI AGCT 5 cut(s) 41, 66, 73, 432, 447
Alw21I GWGCWC 1 cut(s) 811
AlwI GGATC 2 cut(s) 213, 750
AoxI GGCC 3 cut(s) 85, 100, 475
ApaI GGGCCC 1 cut(s) 479
ApeKI GCWGC 4 cut(s) 326, 349, 733, 757
ApoI RAATTY 1 cut(s) 601
AspLEI GCGC 3 cut(s) 254, 370, 372
AspS9I GGNCC 5 cut(s) 313, 421, 475, 476, 538
AsuC2I CCSGG 1 cut(s) 804
AsuHPI GGTGA 5 cut(s) 97, 373, 775, 785, 865
AvaII GGWCC 3 cut(s) 313, 421, 538
BaeGI GKGCMC 2 cut(s) 395, 479
BaeI ACNNNNGTAYC 2 cut(s) 384, 417
BanII GRGCYC 1 cut(s) 479
BauI CACGAG 1 cut(s) 344
Bbv12I GWGCWC 1 cut(s) 811
BbvI GCAGC 4 cut(s) 338, 361, 720, 744
BccI CCATC 8 cut(s) 119, 200, 225, 239, 307, 338, 382, 640
BceAI ACGGC 2 cut(s) 307, 569
BciT130I CCWGG 3 cut(s) 311, 419, 717
BcnI CCSGG 1 cut(s) 804
BfmI CTRYAG 2 cut(s) 427, 758
BfuAI ACCTGC 1 cut(s) 751
BisI GCNGC 8 cut(s) 223, 250, 259, 327, 350, 441, 734, 758
BlsI GCNGC 8 cut(s) 224, 251, 260, 328, 351, 442, 735, 759
Bme1390I CCNGG 4 cut(s) 311, 419, 717, 804
Bme18I GGWCC 3 cut(s) 313, 421, 538
BmgBI CACGTC 1 cut(s) 627
BmgT120I GGNCC 5 cut(s) 313, 421, 475, 476, 538
BmiI GGNNCC 3 cut(s) 477, 492, 539
BmrFI CCNGG 4 cut(s) 311, 419, 717, 804
BmsI GCATC 1 cut(s) 303
BpmI CTGGAG 1 cut(s) 469
BpuMI CCSGG 1 cut(s) 804
BsaJI CCNNGG 2 cut(s) 715, 802
BsaXI ACNNNNNCTCC 4 cut(s) 138, 168, 611, 641
Bsc4I CCNNNNNNNGG 3 cut(s) 447, 450, 803
Bse118I RCCGGY 1 cut(s) 487
Bse1I ACTGG 1 cut(s) 30
BseBI CCWGG 3 cut(s) 311, 419, 717
BseDI CCNNGG 2 cut(s) 715, 802
BseGI GGATG 5 cut(s) 111, 126, 294, 299, 529
BseLI CCNNNNNNNGG 3 cut(s) 447, 450, 803
BseNI ACTGG 1 cut(s) 30
BsePI GCGCGC 1 cut(s) 368
BseRI GAGGAG 1 cut(s) 217
BseSI GKGCMC 2 cut(s) 395, 479
BseXI GCAGC 4 cut(s) 338, 361, 720, 744
BseYI CCCAGC 2 cut(s) 556, 592
Bsh1236I CGCG 3 cut(s) 203, 252, 370
Bsh1285I CGRYCG 1 cut(s) 324
BshFI GGCC 3 cut(s) 87, 102, 477
BsiEI CGRYCG 1 cut(s) 324
BsiHKAI GWGCWC 1 cut(s) 811
BsiSI CCGG 3 cut(s) 488, 506, 804
BslFI GGGAC 4 cut(s) 350, 467, 551, 785
BslI CCNNNNNNNGG 3 cut(s) 447, 450, 803
BsmFI GGGAC 4 cut(s) 350, 467, 551, 785
BsnI GGCC 3 cut(s) 87, 102, 477
Bsp120I GGGCCC 1 cut(s) 475
Bsp1286I GDGCHC 3 cut(s) 395, 479, 811
Bsp143I GATC 7 cut(s) 15, 205, 279, 321, 546, 682, 742
BspANI GGCC 3 cut(s) 87, 102, 477
BspFNI CGCG 3 cut(s) 203, 252, 370
BspLI GGNNCC 3 cut(s) 477, 492, 539
BspMAI CTGCAG 1 cut(s) 762
BspMI ACCTGC 1 cut(s) 751
BspPI GGATC 2 cut(s) 213, 750
BsrBI CCGCTC 1 cut(s) 225
BsrFI RCCGGY 1 cut(s) 487
BsrI ACTGG 1 cut(s) 30
BssAI RCCGGY 1 cut(s) 487
BssECI CCNNGG 2 cut(s) 715, 802
BssHII GCGCGC 1 cut(s) 368
BssMI GATC 7 cut(s) 15, 205, 279, 321, 546, 682, 742
BssSI CACGAG 1 cut(s) 344
Bst2BI CACGAG 1 cut(s) 344
Bst2UI CCWGG 3 cut(s) 311, 419, 717
Bst4CI ACNGT 5 cut(s) 380, 542, 691, 730, 773
BstC8I GCNNGC 5 cut(s) 256, 370, 445, 583, 674
BstDEI CTNAG 1 cut(s) 606
BstF5I GGATG 5 cut(s) 111, 126, 294, 299, 529
BstFNI CGCG 3 cut(s) 203, 252, 370
BstHHI GCGC 3 cut(s) 254, 370, 372
BstKTI GATC 7 cut(s) 18, 208, 282, 324, 549, 685, 745
BstMBI GATC 7 cut(s) 15, 205, 279, 321, 546, 682, 742
BstMCI CGRYCG 1 cut(s) 324
BstMWI GCNNNNNNNGC 3 cut(s) 255, 258, 730
BstNI CCWGG 3 cut(s) 311, 419, 717
BstSCI CCNGG 4 cut(s) 309, 417, 715, 802
BstSFI CTRYAG 2 cut(s) 427, 758
BstSLI GKGCMC 2 cut(s) 395, 479
BstUI CGCG 3 cut(s) 203, 252, 370
BstV1I GCAGC 4 cut(s) 338, 361, 720, 744
BstX2I RGATCY 1 cut(s) 205
BstYI RGATCY 1 cut(s) 205
BsuRI GGCC 3 cut(s) 87, 102, 477
BtgZI GCGATG 2 cut(s) 203, 659
BtrI CACGTC 1 cut(s) 627
BtsCI GGATG 5 cut(s) 111, 126, 294, 299, 529
BtsI GCAGTG 1 cut(s) 507
BtsIMutI CAGTG 1 cut(s) 507
BveI ACCTGC 1 cut(s) 751
Cac8I GCNNGC 5 cut(s) 256, 370, 445, 583, 674
CfoI GCGC 3 cut(s) 254, 370, 372
Cfr10I RCCGGY 1 cut(s) 487
Cfr13I GGNCC 5 cut(s) 313, 421, 475, 476, 538
Csp6I GTAC 1 cut(s) 769
CviAII CATG 1 cut(s) 748
CviQI GTAC 1 cut(s) 769
DdeI CTNAG 1 cut(s) 606
DpnI GATC 7 cut(s) 17, 207, 281, 323, 548, 684, 744
DpnII GATC 7 cut(s) 15, 205, 279, 321, 546, 682, 742
EciI GGCGGA 1 cut(s) 474
Eco147I AGGCCT 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 479
Eco32I GATATC 1 cut(s) 178
Eco47I GGWCC 3 cut(s) 313, 421, 538
Eco57I CTGAAG 2 cut(s) 516, 722
EcoRI GAATTC 1 cut(s) 601
EcoRII CCWGG 3 cut(s) 309, 417, 715
EcoRV GATATC 1 cut(s) 178
EcoT38I GRGCYC 1 cut(s) 479
FaeI CATG 1 cut(s) 751
FaiI YATR 4 cut(s) 165, 572, 667, 749
FaqI GGGAC 4 cut(s) 350, 467, 551, 785
FatI CATG 1 cut(s) 747
FauI CCCGC 4 cut(s) 125, 141, 609, 681
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 8 cut(s) 223, 250, 259, 327, 350, 441, 734, 758
FokI GGATG 5 cut(s) 98, 113, 281, 286, 536
FriOI GRGCYC 1 cut(s) 479
Fsp4HI GCNGC 8 cut(s) 223, 250, 259, 327, 350, 441, 734, 758
GlaI GCGC 3 cut(s) 253, 369, 371
GluI GCNGC 8 cut(s) 223, 250, 259, 327, 350, 441, 734, 758
GsaI CCCAGC 2 cut(s) 560, 596
GsuI CTGGAG 1 cut(s) 469
HaeIII GGCC 3 cut(s) 87, 102, 477
HapII CCGG 3 cut(s) 488, 506, 804
HhaI GCGC 3 cut(s) 254, 370, 372
Hin1II CATG 1 cut(s) 751
Hin6I GCGC 3 cut(s) 252, 368, 370
HinP1I GCGC 3 cut(s) 252, 368, 370
HincII GTYRAC 2 cut(s) 94, 238
HindII GTYRAC 2 cut(s) 94, 238
HinfI GANTC 4 cut(s) 78, 95, 140, 481
HpaII CCGG 3 cut(s) 488, 506, 804
HphI GGTGA 5 cut(s) 97, 373, 775, 785, 865
Hpy166II GTNNAC 2 cut(s) 94, 238
Hpy188I TCNGA 3 cut(s) 77, 158, 712
Hpy188III TCNNGA 4 cut(s) 277, 494, 632, 740
Hpy8I GTNNAC 2 cut(s) 94, 238
Hpy99I CGWCG 2 cut(s) 239, 358
HpyAV CCTTC 2 cut(s) 581, 629
HpyCH4III ACNGT 5 cut(s) 380, 542, 691, 730, 773
HpyCH4IV ACGT 3 cut(s) 263, 626, 822
HpyCH4V TGCA 1 cut(s) 760
HpyF10VI GCNNNNNNNGC 3 cut(s) 255, 258, 730
HpyF3I CTNAG 1 cut(s) 606
HpySE526I ACGT 3 cut(s) 263, 626, 822
Hsp92II CATG 1 cut(s) 751
HspAI GCGC 3 cut(s) 252, 368, 370
Kzo9I GATC 7 cut(s) 15, 205, 279, 321, 546, 682, 742
LmnI GCTCC 2 cut(s) 71, 230
Lsp1109I GCAGC 4 cut(s) 338, 361, 720, 744
LweI GCATC 1 cut(s) 303
MaeII ACGT 3 cut(s) 263, 626, 822
MalI GATC 7 cut(s) 17, 207, 281, 323, 548, 684, 744
MbiI CCGCTC 1 cut(s) 225
MboI GATC 7 cut(s) 15, 205, 279, 321, 546, 682, 742
MboII GAAGA 4 cut(s) 115, 200, 265, 827
MflI RGATCY 1 cut(s) 205
MhlI GDGCHC 3 cut(s) 395, 479, 811
MluCI AATT 4 cut(s) 56, 601, 655, 839
MlyI GAGTC 2 cut(s) 87, 89
MmeI TCCRAC 2 cut(s) 181, 438
MslI CAYNNNNRTG 1 cut(s) 750
MspA1I CMGCKG 1 cut(s) 447
MspI CCGG 3 cut(s) 488, 506, 804
MspR9I CCNGG 4 cut(s) 311, 419, 717, 804
MvaI CCWGG 3 cut(s) 311, 419, 717
MvnI CGCG 3 cut(s) 203, 252, 370
MwoI GCNNNNNNNGC 3 cut(s) 255, 258, 730
NciI CCSGG 1 cut(s) 804
NdeII GATC 7 cut(s) 15, 205, 279, 321, 546, 682, 742
NlaIII CATG 1 cut(s) 751
NlaIV GGNNCC 3 cut(s) 477, 492, 539
NmeAIII GCCGAG 1 cut(s) 542
OliI CACNNNNGTG 1 cut(s) 750
PaqCI CACCTGC 1 cut(s) 751
PauI GCGCGC 1 cut(s) 368
PceI AGGCCT 1 cut(s) 87
PcsI WCGNNNNNNNCGW 2 cut(s) 401, 819
PfeI GAWTC 2 cut(s) 140, 481
PfoI TCCNGGA 1 cut(s) 309
PkrI GCNGC 8 cut(s) 224, 251, 260, 328, 351, 442, 735, 759
Ple19I CGATCG 1 cut(s) 324
PleI GAGTC 2 cut(s) 86, 89
PpsI GAGTC 2 cut(s) 86, 89
Psp1406I AACGTT 1 cut(s) 822
Psp6I CCWGG 3 cut(s) 309, 417, 715
PspFI CCCAGC 2 cut(s) 556, 592
PspGI CCWGG 3 cut(s) 309, 417, 715
PspN4I GGNNCC 3 cut(s) 477, 492, 539
PspOMI GGGCCC 1 cut(s) 475
PspPI GGNCC 5 cut(s) 313, 421, 475, 476, 538
PstI CTGCAG 1 cut(s) 762
PsuI RGATCY 1 cut(s) 205
PteI GCGCGC 1 cut(s) 368
PvuI CGATCG 1 cut(s) 324
PvuII CAGCTG 1 cut(s) 447
RsaI GTAC 1 cut(s) 770
RsaNI GTAC 1 cut(s) 769
RseI CAYNNNNRTG 1 cut(s) 750
SalI GTCGAC 1 cut(s) 236
SatI GCNGC 8 cut(s) 223, 250, 259, 327, 350, 441, 734, 758
Sau3AI GATC 7 cut(s) 15, 205, 279, 321, 546, 682, 742
Sau96I GGNCC 5 cut(s) 313, 421, 475, 476, 538
SchI GAGTC 2 cut(s) 87, 89
ScrFI CCNGG 4 cut(s) 311, 419, 717, 804
SduI GDGCHC 3 cut(s) 395, 479, 811
SfaNI GCATC 1 cut(s) 303
SfcI CTRYAG 2 cut(s) 427, 758
SinI GGWCC 3 cut(s) 313, 421, 538
SmiMI CAYNNNNRTG 1 cut(s) 750
Sse9I AATT 4 cut(s) 56, 601, 655, 839
SseBI AGGCCT 1 cut(s) 87
StuI AGGCCT 1 cut(s) 87
StyD4I CCNGG 4 cut(s) 309, 417, 715, 802
TaaI ACNGT 5 cut(s) 380, 542, 691, 730, 773
TaiI ACGT 3 cut(s) 266, 629, 825
TaqI TCGA 3 cut(s) 60, 195, 237
TasI AATT 4 cut(s) 56, 601, 655, 839
TauI GCSGC 4 cut(s) 225, 252, 261, 443
TfiI GAWTC 2 cut(s) 140, 481
TscAI CASTG 1 cut(s) 507
TseI GCWGC 4 cut(s) 326, 349, 733, 757
TspDTI ATGAA 1 cut(s) 115
TspGWI ACGGA 2 cut(s) 292, 371
TspRI CASTG 1 cut(s) 507
VpaK11BI GGWCC 3 cut(s) 313, 421, 538
XapI RAATTY 1 cut(s) 601
XmiI GTMKAC 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.