RLG00000020853

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001116
Physical Location & Seq
Reverse (-)
69454191 .. 69454604
414 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020853

Sequence Viewer

Length: 309 bp
ATGGTCGACCAAGTCAGCAGCGCGTGCTGCAACTTCGGTTTCTTTCAGATCACCAACCACGGCATACCGTTGGTGGTTCTCGACCGTACGATCGCAGCCATCAAGGCCTTCCACGAGCAGCCGACGGAGGTAAGGCTCGGCCCTGTTCCGGCGGCCGTCGACGAAGTTTCGGAGATTTGTAGAGATGAAATTGTGGTGTGGAACAAGGAGATCCAAAGGCTGGTAGAGGAGCTGCTGGGGCTGGCAAGTGAGGGGCCGGGATTGCTCGCAGAGAAGCTGAAGGCGATTGGATCTGTAGTTGACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

103

Amino Acids

11.07

Weight (kDa)

4.63

Isoelectric Point (pI)

29.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 2 - 45 2.5e-10 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 220
AccI GTMKAC 2 cut(s) 6, 159
AccII CGCG 1 cut(s) 23
AciI CCGC 1 cut(s) 152
AclWI GGATC 2 cut(s) 205, 298
AcoI YGGCCR 1 cut(s) 153
AcuI CTGAAG 1 cut(s) 299
AfaI GTAC 1 cut(s) 88
AfiI CCNNNNNNNGG 2 cut(s) 148, 220
AluBI AGCT 2 cut(s) 232, 277
AluI AGCT 2 cut(s) 232, 277
AlwI GGATC 2 cut(s) 205, 298
AoxI GGCC 4 cut(s) 105, 139, 153, 254
ApeKI GCWGC 5 cut(s) 18, 27, 95, 118, 232
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 2 cut(s) 140, 254
AsuC2I CCSGG 1 cut(s) 258
AsuHPI GGTGA 1 cut(s) 43
BauI CACGAG 1 cut(s) 113
BbvI GCAGC 5 cut(s) 14, 30, 107, 130, 219
BccI CCATC 1 cut(s) 107
BceAI ACGGC 2 cut(s) 76, 140
BcnI CCSGG 1 cut(s) 258
BfmI CTRYAG 1 cut(s) 294
BglI GCCNNNNNGGC 1 cut(s) 104
BisI GCNGC 6 cut(s) 19, 28, 96, 119, 153, 233
BlsI GCNGC 6 cut(s) 20, 29, 97, 120, 154, 234
Bme1390I CCNGG 1 cut(s) 258
BmgT120I GGNCC 2 cut(s) 140, 254
BmiI GGNNCC 1 cut(s) 255
BmrFI CCNGG 1 cut(s) 258
BpuMI CCSGG 1 cut(s) 258
BsaJI CCNNGG 1 cut(s) 58
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 220
BseDI CCNNGG 1 cut(s) 58
BseLI CCNNNNNNNGG 2 cut(s) 148, 220
BseRI GAGGAG 1 cut(s) 242
BseX3I CGGCCG 1 cut(s) 153
BseXI GCAGC 5 cut(s) 14, 30, 107, 130, 219
BseYI CCCAGC 1 cut(s) 235
Bsh1236I CGCG 1 cut(s) 23
Bsh1285I CGRYCG 3 cut(s) 85, 93, 156
BshFI GGCC 4 cut(s) 107, 141, 155, 256
BsiEI CGRYCG 3 cut(s) 85, 93, 156
BsiSI CCGG 2 cut(s) 149, 257
BsiWI CGTACG 1 cut(s) 86
BslI CCNNNNNNNGG 2 cut(s) 148, 220
BsnI GGCC 4 cut(s) 107, 141, 155, 256
Bsp143I GATC 4 cut(s) 48, 90, 210, 290
BspACI CCGC 1 cut(s) 152
BspANI GGCC 4 cut(s) 107, 141, 155, 256
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 1 cut(s) 255
BspPI GGATC 2 cut(s) 205, 298
BssECI CCNNGG 1 cut(s) 58
BssMI GATC 4 cut(s) 48, 90, 210, 290
BssSI CACGAG 1 cut(s) 113
Bst2BI CACGAG 1 cut(s) 113
Bst4CI ACNGT 2 cut(s) 69, 86
BstAPI GCANNNNNTGC 1 cut(s) 24
BstC8I GCNNGC 3 cut(s) 25, 243, 267
BstDSI CCRYGG 1 cut(s) 58
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 4 cut(s) 51, 93, 213, 293
BstMBI GATC 4 cut(s) 48, 90, 210, 290
BstMCI CGRYCG 3 cut(s) 85, 93, 156
BstMWI GCNNNNNNNGC 5 cut(s) 24, 27, 104, 238, 262
BstSCI CCNGG 1 cut(s) 256
BstSFI CTRYAG 1 cut(s) 294
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 5 cut(s) 14, 30, 107, 130, 219
BstX2I RGATCY 2 cut(s) 210, 290
BstYI RGATCY 2 cut(s) 210, 290
BstZI CGGCCG 1 cut(s) 153
BsuRI GGCC 4 cut(s) 107, 141, 155, 256
BtgI CCRYGG 1 cut(s) 58
Cac8I GCNNGC 3 cut(s) 25, 243, 267
CfoI GCGC 1 cut(s) 23
Cfr13I GGNCC 2 cut(s) 140, 254
Csp6I GTAC 1 cut(s) 87
CviQI GTAC 1 cut(s) 87
DpnI GATC 4 cut(s) 50, 92, 212, 292
DpnII GATC 4 cut(s) 48, 90, 210, 290
EaeI YGGCCR 1 cut(s) 153
EagI CGGCCG 1 cut(s) 153
EclXI CGGCCG 1 cut(s) 153
Eco147I AGGCCT 1 cut(s) 107
Eco52I CGGCCG 1 cut(s) 153
Eco57I CTGAAG 1 cut(s) 299
FaiI YATR 1 cut(s) 65
FblI GTMKAC 2 cut(s) 6, 159
Fnu4HI GCNGC 6 cut(s) 19, 28, 96, 119, 153, 233
Fsp4HI GCNGC 6 cut(s) 19, 28, 96, 119, 153, 233
GlaI GCGC 1 cut(s) 22
GluI GCNGC 6 cut(s) 19, 28, 96, 119, 153, 233
GsaI CCCAGC 1 cut(s) 239
HaeIII GGCC 4 cut(s) 107, 141, 155, 256
HapII CCGG 2 cut(s) 149, 257
HhaI GCGC 1 cut(s) 23
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HincII GTYRAC 3 cut(s) 7, 160, 301
HindII GTYRAC 3 cut(s) 7, 160, 301
HpaII CCGG 2 cut(s) 149, 257
HphI GGTGA 1 cut(s) 43
Hpy166II GTNNAC 3 cut(s) 7, 160, 301
Hpy188I TCNGA 2 cut(s) 48, 172
Hpy188III TCNNGA 1 cut(s) 80
Hpy8I GTNNAC 3 cut(s) 7, 160, 301
Hpy99I CGWCG 3 cut(s) 127, 161, 164
HpyAV CCTTC 2 cut(s) 118, 274
HpyCH4III ACNGT 2 cut(s) 69, 86
HpyCH4V TGCA 1 cut(s) 30
HpyF10VI GCNNNNNNNGC 5 cut(s) 24, 27, 104, 238, 262
HspAI GCGC 1 cut(s) 21
Kzo9I GATC 4 cut(s) 48, 90, 210, 290
LmnI GCTCC 1 cut(s) 229
LpnPI CCDG 6 cut(s) 156, 162, 206, 221, 227, 270
Lsp1109I GCAGC 5 cut(s) 14, 30, 107, 130, 219
MalI GATC 4 cut(s) 50, 92, 212, 292
MboI GATC 4 cut(s) 48, 90, 210, 290
MflI RGATCY 2 cut(s) 210, 290
MluCI AATT 1 cut(s) 189
MnlI CCTC 3 cut(s) 121, 220, 244
MspI CCGG 2 cut(s) 149, 257
MspR9I CCNGG 1 cut(s) 258
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 5 cut(s) 24, 27, 104, 238, 262
NciI CCSGG 1 cut(s) 258
NdeII GATC 4 cut(s) 48, 90, 210, 290
NlaIV GGNNCC 1 cut(s) 255
NmeAIII GCCGAG 1 cut(s) 117
PceI AGGCCT 1 cut(s) 107
Pfl23II CGTACG 1 cut(s) 86
PflFI GACNNNGTC 1 cut(s) 11
PflMI CCANNNNNTGG 1 cut(s) 220
PkrI GCNGC 6 cut(s) 20, 29, 97, 120, 154, 234
Ple19I CGATCG 1 cut(s) 93
PspFI CCCAGC 1 cut(s) 235
PspLI CGTACG 1 cut(s) 86
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 2 cut(s) 140, 254
PsuI RGATCY 2 cut(s) 210, 290
PsyI GACNNNGTC 1 cut(s) 11
PvuI CGATCG 1 cut(s) 93
RsaI GTAC 1 cut(s) 88
RsaNI GTAC 1 cut(s) 87
SalI GTCGAC 2 cut(s) 5, 158
SatI GCNGC 6 cut(s) 19, 28, 96, 119, 153, 233
Sau3AI GATC 4 cut(s) 48, 90, 210, 290
Sau96I GGNCC 2 cut(s) 140, 254
ScrFI CCNGG 1 cut(s) 258
SetI ASST 3 cut(s) 132, 234, 279
SfcI CTRYAG 1 cut(s) 294
SgrDI CGTCGACG 1 cut(s) 158
Sse9I AATT 1 cut(s) 189
SseBI AGGCCT 1 cut(s) 107
SsiI CCGC 1 cut(s) 152
StuI AGGCCT 1 cut(s) 107
StyD4I CCNGG 1 cut(s) 256
TaaI ACNGT 2 cut(s) 69, 86
TaqI TCGA 3 cut(s) 6, 81, 159
TasI AATT 1 cut(s) 189
TauI GCSGC 1 cut(s) 155
TseI GCWGC 5 cut(s) 18, 27, 95, 118, 232
TspDTI ATGAA 1 cut(s) 201
TspGWI ACGGA 1 cut(s) 140
Tth111I GACNNNGTC 1 cut(s) 11
Van91I CCANNNNNTGG 1 cut(s) 220
XmiI GTMKAC 2 cut(s) 6, 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.