Rw4G034480

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001116
Physical Location & Seq
Reverse (-)
61532608 .. 61533781
1174 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G034480.1

Sequence Viewer

Length: 1098 bp
ATGGCGGCAAATGGTACCTTGATCGGCGAACAGGGTTATGACCGAGCTGAAGAAGTGAAGCAATTCGACGACTCCAAGCTCGGAGTCAAAGGCCTCATCGACTCGGGTCTCTCCTCCATCCCCCGAATCTTTATCCACCCGCCCGAAACATTACAGCAATTCCAGCCCACAACCCGACCCAACGCCCAACCAATCCCCACCATCGACCTCTCCGACCTCCACTCCGATCGTCGCTCCTCCATCGTTGACCAGGTCAGTGGCGCGTGCCGCGACTTCGGTTTCTTCCAGATCACCAACCACGGAATACCGTCGGTGGTTCTCGACCGTACGATCGCCGCCGTCAAGGCCTTCCACGAGCAGCCGACGGAGGTCAAGGCGCGGTTCTACCGTAGAGAGATGGACCTGGGGGTTTCGTATTTCTCCAACATCGACCTGTACACGTCCAAAGCCGCCAGCTGGCGCGACACGCTTCAGATGAGGCTCAGCCCCGTGCCGCCCGCCGCCGACGACGTTCCGGAGGTTTGTAGGGACGAAATTGCGGCATGGAACCAGCAGATCCAACGGCTGGGAGAGGAACTGCTGGGGCTGGTGAGTGAAGGGCTGGGATTGCCTGCGGAGAAGCTGAAGGAGATGACGTGTCTGGAAGGGAGGGCGATGGTGGGCAACTACTACCCGCCCTGCCCGCAACCTGACCTCACCGTAGGGCTTACGTCACACAGTGACCCTGGAGTGTTGACCGTGCTGCTTCAAGATCACCTTGGTGGGTTGCAGGTTAAGCACGAGGATGCTTGGTTGGATGTGAAACCCGTCTCCGGTGCTCTTGTAATCAACGTTGGTGATTTACTCCAGATTATTTCAAACGAGGAATACAAAAGCGTGGATCACCGGGTGATGGCGAATCCACAACACGAGGCGCGTGTCGCAATAGCCGTGTTTTTGAATCCGAGCAATAGAGAGGGCGTGTTTGGTCCGTTGCCGGAGCTGGTCTCGTCAGAGAAGCCAGCTCTCTACAGAGAGTTCAAGTTTACGGAATTTATAATGAGGTTCTTCAAGAAAGAATTGAATGGCAAATCTTTGGTAAATTATTATAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

365

Amino Acids

40.67

Weight (kDa)

5.37

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 65 - 162 5.2e-20 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 218 - 315 1.1e-31 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1037, 1089
Acc36I ACCTGC 1 cut(s) 760
Acc65I GGTACC 1 cut(s) 14
AccB1I GGYRCC 1 cut(s) 14
AccB7I CCANNNNNTGG 1 cut(s) 565
AccII CGCG 5 cut(s) 263, 270, 379, 462, 916
AccIII TCCGGA 1 cut(s) 514
AclI AACGTT 1 cut(s) 831
AclWI GGATC 2 cut(s) 550, 888
AcsI RAATTY 1 cut(s) 1031
AcuI CTGAAG 3 cut(s) 69, 455, 644
AdeI CACNNNGTG 2 cut(s) 719, 889
AfaI GTAC 3 cut(s) 16, 328, 437
AfiI CCNNNNNNNGG 4 cut(s) 456, 565, 812, 892
AflIII ACRYGT 2 cut(s) 438, 635
AgsI TTSAA 6 cut(s) 749, 858, 940, 1021, 1051, 1063
AjiI CACGTC 2 cut(s) 441, 636
AjnI CCWGG 3 cut(s) 249, 402, 724
AleI CACNNNNGTG 1 cut(s) 759
AluBI AGCT 6 cut(s) 47, 79, 456, 622, 982, 1004
AluI AGCT 6 cut(s) 47, 79, 456, 622, 982, 1004
Alw21I GWGCWC 1 cut(s) 820
Alw26I GTCTC 3 cut(s) 113, 814, 991
AlwI GGATC 2 cut(s) 550, 888
Ama87I CYCGRG 1 cut(s) 103
Aor13HI TCCGGA 1 cut(s) 514
AoxI GGCC 2 cut(s) 91, 345
ApeKI GCWGC 2 cut(s) 358, 742
ApoI RAATTY 1 cut(s) 1031
Asp700I GAANNNNTTC 1 cut(s) 62
Asp718I GGTACC 1 cut(s) 14
AspLEI GCGC 4 cut(s) 263, 379, 462, 916
AspS9I GGNCC 2 cut(s) 400, 968
AsuC2I CCSGG 1 cut(s) 887
AsuHPI GGTGA 7 cut(s) 283, 601, 688, 746, 848, 875, 901
AvaI CYCGRG 1 cut(s) 103
AvaII GGWCC 2 cut(s) 400, 968
BanI GGYRCC 1 cut(s) 14
BauI CACGAG 3 cut(s) 353, 779, 908
Bbv12I GWGCWC 1 cut(s) 820
BbvI GCAGC 2 cut(s) 370, 729
BccI CCATC 6 cut(s) 125, 209, 248, 391, 649, 886
BceAI ACGGC 3 cut(s) 323, 578, 914
BciT130I CCWGG 3 cut(s) 251, 404, 726
BcnI CCSGG 1 cut(s) 887
BcoDI GTCTC 3 cut(s) 113, 814, 991
BfmI CTRYAG 1 cut(s) 1009
BfuAI ACCTGC 1 cut(s) 760
BglI GCCNNNNNGGC 1 cut(s) 344
BisI GCNGC 9 cut(s) 6, 268, 336, 359, 450, 494, 501, 540, 743
BlpI GCTNAGC 1 cut(s) 482
BlsI GCNGC 9 cut(s) 7, 269, 337, 360, 451, 495, 502, 541, 744
Bme1390I CCNGG 4 cut(s) 251, 404, 726, 887
Bme18I GGWCC 2 cut(s) 400, 968
BmeT110I CYCGRG 1 cut(s) 103
BmgBI CACGTC 2 cut(s) 441, 636
BmgT120I GGNCC 2 cut(s) 400, 968
BmiI GGNNCC 2 cut(s) 16, 548
BmrFI CCNGG 4 cut(s) 251, 404, 726, 887
BmsI GCATC 1 cut(s) 775
BoxI GACNNNNGTC 2 cut(s) 105, 368
BplI GAGNNNNNCTC 2 cut(s) 971, 1003
BpmI CTGGAG 2 cut(s) 747, 830
Bpu1102I GCTNAGC 1 cut(s) 482
BpuMI CCSGG 1 cut(s) 887
BsaI GGTCTC 2 cut(s) 113, 991
BsaJI CCNNGG 4 cut(s) 298, 403, 724, 757
BsaWI WCCGGW 2 cut(s) 514, 812
BsaXI ACNNNNNCTCC 4 cut(s) 206, 236, 620, 650
Bsc4I CCNNNNNNNGG 4 cut(s) 456, 565, 812, 892
BseAI TCCGGA 1 cut(s) 514
BseBI CCWGG 3 cut(s) 251, 404, 726
BseDI CCNNGG 4 cut(s) 298, 403, 724, 757
BseGI GGATG 3 cut(s) 117, 790, 802
BseLI CCNNNNNNNGG 4 cut(s) 456, 565, 812, 892
BseMII CTCAG 1 cut(s) 496
BseRI GAGGAG 2 cut(s) 103, 226
BseXI GCAGC 2 cut(s) 370, 729
BseYI CCCAGC 3 cut(s) 565, 580, 601
Bsh1236I CGCG 5 cut(s) 263, 270, 379, 462, 916
Bsh1285I CGRYCG 3 cut(s) 229, 325, 333
BshFI GGCC 2 cut(s) 93, 347
BshNI GGYRCC 1 cut(s) 14
BsiEI CGRYCG 3 cut(s) 229, 325, 333
BsiHKAI GWGCWC 1 cut(s) 820
BsiHKCI CYCGRG 1 cut(s) 103
BsiSI CCGG 4 cut(s) 515, 813, 886, 977
BsiWI CGTACG 1 cut(s) 326
BslFI GGGAC 1 cut(s) 542
BslI CCNNNNNNNGG 4 cut(s) 456, 565, 812, 892
BsmAI GTCTC 3 cut(s) 113, 814, 991
BsmBI CGTCTC 1 cut(s) 814
BsmFI GGGAC 1 cut(s) 542
BsnI GGCC 2 cut(s) 93, 347
Bso31I GGTCTC 2 cut(s) 113, 991
BsoBI CYCGRG 1 cut(s) 103
Bsp1286I GDGCHC 1 cut(s) 820
Bsp13I TCCGGA 1 cut(s) 514
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 7 cut(s) 21, 226, 288, 330, 555, 751, 880
Bsp1720I GCTNAGC 1 cut(s) 482
BspANI GGCC 2 cut(s) 93, 347
BspCNI CTCAG 1 cut(s) 495
BspEI TCCGGA 1 cut(s) 514
BspFNI CGCG 5 cut(s) 263, 270, 379, 462, 916
BspLI GGNNCC 2 cut(s) 16, 548
BspMI ACCTGC 1 cut(s) 760
BspPI GGATC 2 cut(s) 550, 888
BspT107I GGYRCC 1 cut(s) 14
BspTNI GGTCTC 2 cut(s) 113, 991
BsrGI TGTACA 1 cut(s) 435
BssECI CCNNGG 4 cut(s) 298, 403, 724, 757
BssMI GATC 7 cut(s) 21, 226, 288, 330, 555, 751, 880
BssSI CACGAG 3 cut(s) 353, 779, 908
BssT1I CCWWGG 1 cut(s) 757
Bst2BI CACGAG 3 cut(s) 353, 779, 908
Bst2UI CCWGG 3 cut(s) 251, 404, 726
Bst4CI ACNGT 6 cut(s) 309, 326, 389, 700, 719, 739
BstAUI TGTACA 1 cut(s) 435
BstC8I GCNNGC 7 cut(s) 265, 454, 458, 498, 612, 683, 1002
BstDEI CTNAG 1 cut(s) 482
BstDSI CCRYGG 1 cut(s) 298
BstF5I GGATG 3 cut(s) 117, 790, 802
BstFNI CGCG 5 cut(s) 263, 270, 379, 462, 916
BstHHI GCGC 4 cut(s) 263, 379, 462, 916
BstKTI GATC 7 cut(s) 24, 229, 291, 333, 558, 754, 883
BstMAI GTCTC 3 cut(s) 113, 814, 991
BstMBI GATC 7 cut(s) 21, 226, 288, 330, 555, 751, 880
BstMCI CGRYCG 3 cut(s) 229, 325, 333
BstMWI GCNNNNNNNGC 8 cut(s) 163, 267, 344, 466, 607, 682, 775, 920
BstNI CCWGG 3 cut(s) 251, 404, 726
BstPAI GACNNNNGTC 2 cut(s) 105, 368
BstSCI CCNGG 4 cut(s) 249, 402, 724, 885
BstSFI CTRYAG 1 cut(s) 1009
BstUI CGCG 5 cut(s) 263, 270, 379, 462, 916
BstV1I GCAGC 2 cut(s) 370, 729
BstX2I RGATCY 1 cut(s) 555
BstXI CCANNNNNNTGG 1 cut(s) 257
BstYI RGATCY 1 cut(s) 555
BsuRI GGCC 2 cut(s) 93, 347
BtgI CCRYGG 1 cut(s) 298
BtgZI GCGATG 1 cut(s) 668
BtrI CACGTC 2 cut(s) 441, 636
BtsCI GGATG 3 cut(s) 117, 790, 802
BtsIMutI CAGTG 2 cut(s) 262, 724
BveI ACCTGC 1 cut(s) 760
Cac8I GCNNGC 7 cut(s) 265, 454, 458, 498, 612, 683, 1002
CfoI GCGC 4 cut(s) 263, 379, 462, 916
Cfr13I GGNCC 2 cut(s) 400, 968
CsiI ACCWGGT 1 cut(s) 249
Csp6I GTAC 3 cut(s) 15, 327, 436
CviAII CATG 1 cut(s) 543
CviQI GTAC 3 cut(s) 15, 327, 436
DdeI CTNAG 1 cut(s) 482
DpnI GATC 7 cut(s) 23, 228, 290, 332, 557, 753, 882
DpnII GATC 7 cut(s) 21, 226, 288, 330, 555, 751, 880
DraIII CACNNNGTG 2 cut(s) 719, 889
Eco130I CCWWGG 1 cut(s) 757
Eco147I AGGCCT 2 cut(s) 93, 347
Eco31I GGTCTC 2 cut(s) 113, 991
Eco47I GGWCC 2 cut(s) 400, 968
Eco57I CTGAAG 3 cut(s) 69, 455, 644
Eco88I CYCGRG 1 cut(s) 103
EcoRII CCWGG 3 cut(s) 249, 402, 724
EcoT14I CCWWGG 1 cut(s) 757
ErhI CCWWGG 1 cut(s) 757
Esp3I CGTCTC 1 cut(s) 814
FaeI CATG 1 cut(s) 546
FaiI YATR 4 cut(s) 39, 544, 1037, 1089
FalI AAGNNNNNCTT 2 cut(s) 741, 773
FaqI GGGAC 1 cut(s) 542
FatI CATG 1 cut(s) 542
FauI CCCGC 4 cut(s) 147, 505, 681, 690
Fnu4HI GCNGC 9 cut(s) 6, 268, 336, 359, 450, 494, 501, 540, 743
FokI GGATG 3 cut(s) 104, 797, 809
Fsp4HI GCNGC 9 cut(s) 6, 268, 336, 359, 450, 494, 501, 540, 743
GlaI GCGC 4 cut(s) 262, 378, 461, 915
GluI GCNGC 9 cut(s) 6, 268, 336, 359, 450, 494, 501, 540, 743
GsaI CCCAGC 3 cut(s) 569, 584, 605
GsuI CTGGAG 2 cut(s) 747, 830
HaeIII GGCC 2 cut(s) 93, 347
HapII CCGG 4 cut(s) 515, 813, 886, 977
HhaI GCGC 4 cut(s) 263, 379, 462, 916
Hin1II CATG 1 cut(s) 546
Hin6I GCGC 4 cut(s) 261, 377, 460, 914
HinP1I GCGC 4 cut(s) 261, 377, 460, 914
HincII GTYRAC 2 cut(s) 247, 735
HindII GTYRAC 2 cut(s) 247, 735
HinfI GANTC 6 cut(s) 71, 84, 101, 126, 898, 940
HpaII CCGG 4 cut(s) 515, 813, 886, 977
HphI GGTGA 7 cut(s) 283, 601, 688, 746, 848, 875, 901
Hpy166II GTNNAC 4 cut(s) 247, 438, 735, 1026
Hpy188I TCNGA 6 cut(s) 83, 214, 226, 474, 945, 994
Hpy188III TCNNGA 7 cut(s) 286, 320, 515, 641, 749, 847, 1051
Hpy8I GTNNAC 4 cut(s) 247, 438, 735, 1026
Hpy99I CGWCG 6 cut(s) 71, 234, 313, 367, 509, 512
HpyAV CCTTC 4 cut(s) 358, 590, 619, 638
HpyCH4III ACNGT 6 cut(s) 309, 326, 389, 700, 719, 739
HpyCH4IV ACGT 5 cut(s) 440, 510, 635, 710, 831
HpyCH4V TGCA 1 cut(s) 769
HpyF10VI GCNNNNNNNGC 8 cut(s) 163, 267, 344, 466, 607, 682, 775, 920
HpyF3I CTNAG 1 cut(s) 482
HpySE526I ACGT 5 cut(s) 440, 510, 635, 710, 831
Hsp92II CATG 1 cut(s) 546
HspAI GCGC 4 cut(s) 261, 377, 460, 914
Kpn2I TCCGGA 1 cut(s) 514
KpnI GGTACC 1 cut(s) 18
Kzo9I GATC 7 cut(s) 21, 226, 288, 330, 555, 751, 880
LmnI GCTCC 2 cut(s) 239, 979
Lsp1109I GCAGC 2 cut(s) 370, 729
LweI GCATC 1 cut(s) 775
MabI ACCWGGT 1 cut(s) 249
MaeII ACGT 5 cut(s) 440, 510, 635, 710, 831
MaeIII GTNAC 2 cut(s) 711, 719
MalI GATC 7 cut(s) 23, 228, 290, 332, 557, 753, 882
MboI GATC 7 cut(s) 21, 226, 288, 330, 555, 751, 880
MboII GAAGA 3 cut(s) 62, 274, 1039
MflI RGATCY 1 cut(s) 555
MhlI GDGCHC 1 cut(s) 820
MluCI AATT 6 cut(s) 62, 158, 534, 1031, 1058, 1081
MlyI GAGTC 3 cut(s) 65, 93, 95
MmeI TCCRAC 4 cut(s) 237, 447, 583, 774
MroI TCCGGA 1 cut(s) 514
MroXI GAANNNNTTC 1 cut(s) 62
MseI TTAA 1 cut(s) 774
MslI CAYNNNNRTG 2 cut(s) 759, 783
MspA1I CMGCKG 1 cut(s) 456
MspI CCGG 4 cut(s) 515, 813, 886, 977
MspR9I CCNGG 4 cut(s) 251, 404, 726, 887
MvaI CCWGG 3 cut(s) 251, 404, 726
MvnI CGCG 5 cut(s) 263, 270, 379, 462, 916
MwoI GCNNNNNNNGC 8 cut(s) 163, 267, 344, 466, 607, 682, 775, 920
NciI CCSGG 1 cut(s) 887
NdeII GATC 7 cut(s) 21, 226, 288, 330, 555, 751, 880
NlaIII CATG 1 cut(s) 546
NlaIV GGNNCC 2 cut(s) 16, 548
NmuCI GTSAC 2 cut(s) 711, 719
OliI CACNNNNGTG 1 cut(s) 759
PceI AGGCCT 2 cut(s) 93, 347
PcsI WCGNNNNNNNCGW 2 cut(s) 210, 927
PdmI GAANNNNTTC 1 cut(s) 62
PfeI GAWTC 3 cut(s) 126, 898, 940
Pfl23II CGTACG 1 cut(s) 326
PflFI GACNNNGTC 1 cut(s) 251
PflMI CCANNNNNTGG 1 cut(s) 565
PkrI GCNGC 9 cut(s) 7, 269, 337, 360, 451, 495, 502, 541, 744
Ple19I CGATCG 2 cut(s) 229, 333
PleI GAGTC 3 cut(s) 65, 92, 95
PpsI GAGTC 3 cut(s) 65, 92, 95
PshAI GACNNNNGTC 2 cut(s) 105, 368
PsiI TTATAA 2 cut(s) 1037, 1089
Psp1406I AACGTT 1 cut(s) 831
Psp6I CCWGG 3 cut(s) 249, 402, 724
PspFI CCCAGC 3 cut(s) 565, 580, 601
PspGI CCWGG 3 cut(s) 249, 402, 724
PspLI CGTACG 1 cut(s) 326
PspN4I GGNNCC 2 cut(s) 16, 548
PspPI GGNCC 2 cut(s) 400, 968
PsuI RGATCY 1 cut(s) 555
PsyI GACNNNGTC 1 cut(s) 251
PvuI CGATCG 2 cut(s) 229, 333
PvuII CAGCTG 1 cut(s) 456
RsaI GTAC 3 cut(s) 16, 328, 437
RsaNI GTAC 3 cut(s) 15, 327, 436
RseI CAYNNNNRTG 2 cut(s) 759, 783
SaqAI TTAA 1 cut(s) 774
SatI GCNGC 9 cut(s) 6, 268, 336, 359, 450, 494, 501, 540, 743
Sau3AI GATC 7 cut(s) 21, 226, 288, 330, 555, 751, 880
Sau96I GGNCC 2 cut(s) 400, 968
SchI GAGTC 3 cut(s) 65, 93, 95
ScrFI CCNGG 4 cut(s) 251, 404, 726, 887
SduI GDGCHC 1 cut(s) 820
SexAI ACCWGGT 1 cut(s) 249
SfaNI GCATC 1 cut(s) 775
SfcI CTRYAG 1 cut(s) 1009
SinI GGWCC 2 cut(s) 400, 968
SmiMI CAYNNNNRTG 2 cut(s) 759, 783
Sse9I AATT 6 cut(s) 62, 158, 534, 1031, 1058, 1081
SseBI AGGCCT 2 cut(s) 93, 347
StuI AGGCCT 2 cut(s) 93, 347
StyD4I CCNGG 4 cut(s) 249, 402, 724, 885
StyI CCWWGG 1 cut(s) 757
TaaI ACNGT 6 cut(s) 309, 326, 389, 700, 719, 739
TaiI ACGT 5 cut(s) 443, 513, 638, 713, 834
TaqI TCGA 5 cut(s) 66, 99, 204, 321, 429
TaqII GACCGA 1 cut(s) 57
TasI AATT 6 cut(s) 62, 158, 534, 1031, 1058, 1081
TatI WGTACW 1 cut(s) 435
TauI GCSGC 7 cut(s) 8, 270, 338, 452, 496, 503, 542
TfiI GAWTC 3 cut(s) 126, 898, 940
Tru1I TTAA 1 cut(s) 774
Tru9I TTAA 1 cut(s) 774
TscAI CASTG 2 cut(s) 262, 724
TseFI GTSAC 2 cut(s) 711, 719
TseI GCWGC 2 cut(s) 358, 742
Tsp45I GTSAC 2 cut(s) 711, 719
TspGWI ACGGA 4 cut(s) 315, 380, 960, 1043
TspRI CASTG 2 cut(s) 262, 724
Tth111I GACNNNGTC 1 cut(s) 251
Van91I CCANNNNNTGG 1 cut(s) 565
VpaK11BI GGWCC 2 cut(s) 400, 968
XapI RAATTY 1 cut(s) 1031
XmnI GAANNNNTTC 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.