RLG00000008366

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001116
Physical Location & Seq
Reverse (-)
29542695 .. 29543949
1255 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008366

Sequence Viewer

Length: 540 bp
ATGGTCCAAATTGGATCCATGACTGACCCAATTGAACCCTCACTGCAGCACTCCTTTGCTGCTAGCATTGAAAAAAAAAAAGATCATAGACAACTGCGACCCCAACCAATCCCCACCATCGACCTCTCCGACCTCAACTCCGATCGCCGCTCTGCCATCTTGGACCAAGTCAATGGCGCGTGCTGCAACTTCGGTTTCTTCCAGATCACTAACCACGGCATACCGTCGGTGGTTCTCGACAGTACGATCGCCACCGTCGAGGCCTCCCACGAGCAGCCGACGGAGGTCAAGGGGCCGTTCTACCATAGAGAGATGGGCCAGGGCGTATTGTATCTCTCCAACGTCGACCTGTACACCTCTAAAGCCGCCAGCTGGCGCAACACGCTTCAGGTAAGGCTCGGCCCTATTCTAGCGGCCGTTGACGAAGTTCCGGAGATTTGTAGAGATGAAATTGTGGCGTGGAACAAGGAGATCCAACAGCAGGCAGAGGAGCTGCTGGGGCTGGCAAGTGAGGGCTGGGATTGCACGCGGAGAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

180

Amino Acids

19.95

Weight (kDa)

5.06

Isoelectric Point (pI)

56.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 37 - 131 4.9e-15 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 150
AccI GTMKAC 1 cut(s) 345
AccII CGCG 2 cut(s) 179, 529
AccIII TCCGGA 1 cut(s) 430
AciI CCGC 4 cut(s) 148, 366, 413, 529
AclWI GGATC 3 cut(s) 9, 22, 466
AcoI YGGCCR 1 cut(s) 414
AcuI CTGAAG 1 cut(s) 371
AfaI GTAC 2 cut(s) 244, 353
AfiI CCNNNNNNNGG 2 cut(s) 372, 481
AgsI TTSAA 2 cut(s) 35, 71
AjnI CCWGG 1 cut(s) 318
AluBI AGCT 3 cut(s) 372, 493, 537
AluI AGCT 3 cut(s) 372, 493, 537
AlwI GGATC 3 cut(s) 9, 22, 466
Aor13HI TCCGGA 1 cut(s) 430
AoxI GGCC 5 cut(s) 261, 293, 316, 400, 414
ApeKI GCWGC 5 cut(s) 46, 59, 183, 274, 493
AspLEI GCGC 2 cut(s) 179, 378
AspS9I GGNCC 5 cut(s) 4, 163, 293, 316, 401
AsuNHI GCTAGC 1 cut(s) 62
AvaII GGWCC 2 cut(s) 4, 163
BamHI GGATCC 1 cut(s) 14
BauI CACGAG 1 cut(s) 269
BbvI GCAGC 5 cut(s) 46, 58, 170, 286, 480
BccI CCATC 3 cut(s) 125, 164, 307
BceAI ACGGC 3 cut(s) 232, 280, 401
BciT130I CCWGG 1 cut(s) 320
BfaI CTAG 2 cut(s) 63, 410
BfmI CTRYAG 1 cut(s) 44
BisI GCNGC 8 cut(s) 47, 60, 148, 184, 275, 366, 414, 494
BlsI GCNGC 8 cut(s) 48, 61, 149, 185, 276, 367, 415, 495
Bme1390I CCNGG 1 cut(s) 320
Bme18I GGWCC 2 cut(s) 4, 163
BmgT120I GGNCC 5 cut(s) 4, 163, 293, 316, 401
BmiI GGNNCC 2 cut(s) 16, 294
BmrFI CCNGG 1 cut(s) 320
BmtI GCTAGC 1 cut(s) 66
BoxI GACNNNNGTC 1 cut(s) 284
BsaJI CCNNGG 2 cut(s) 214, 319
BsaWI WCCGGW 1 cut(s) 430
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 2 cut(s) 372, 481
BseAI TCCGGA 1 cut(s) 430
BseBI CCWGG 1 cut(s) 320
BseDI CCNNGG 2 cut(s) 214, 319
BseLI CCNNNNNNNGG 2 cut(s) 372, 481
BseRI GAGGAG 1 cut(s) 503
BseX3I CGGCCG 1 cut(s) 414
BseXI GCAGC 5 cut(s) 46, 58, 170, 286, 480
BseYI CCCAGC 2 cut(s) 496, 516
Bsh1236I CGCG 2 cut(s) 179, 529
Bsh1285I CGRYCG 3 cut(s) 145, 249, 417
BshFI GGCC 5 cut(s) 263, 295, 318, 402, 416
BsiEI CGRYCG 3 cut(s) 145, 249, 417
BsiSI CCGG 1 cut(s) 431
BslI CCNNNNNNNGG 2 cut(s) 372, 481
BsnI GGCC 5 cut(s) 263, 295, 318, 402, 416
Bsp13I TCCGGA 1 cut(s) 430
Bsp1407I TGTACA 1 cut(s) 351
Bsp143I GATC 6 cut(s) 14, 82, 142, 204, 246, 471
BspACI CCGC 4 cut(s) 148, 366, 413, 529
BspANI GGCC 5 cut(s) 263, 295, 318, 402, 416
BspEI TCCGGA 1 cut(s) 430
BspFNI CGCG 2 cut(s) 179, 529
BspLI GGNNCC 2 cut(s) 16, 294
BspMAI CTGCAG 1 cut(s) 48
BspOI GCTAGC 1 cut(s) 66
BspPI GGATC 3 cut(s) 9, 22, 466
BsrBI CCGCTC 1 cut(s) 150
BsrGI TGTACA 1 cut(s) 351
BssECI CCNNGG 2 cut(s) 214, 319
BssMI GATC 6 cut(s) 14, 82, 142, 204, 246, 471
BssSI CACGAG 1 cut(s) 269
Bst2BI CACGAG 1 cut(s) 269
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 3 cut(s) 225, 242, 256
BstAUI TGTACA 1 cut(s) 351
BstC8I GCNNGC 7 cut(s) 64, 181, 370, 374, 483, 504, 527
BstDSI CCRYGG 1 cut(s) 214
BstFNI CGCG 2 cut(s) 179, 529
BstHHI GCGC 2 cut(s) 179, 378
BstKTI GATC 6 cut(s) 17, 85, 145, 207, 249, 474
BstMBI GATC 6 cut(s) 14, 82, 142, 204, 246, 471
BstMCI CGRYCG 3 cut(s) 145, 249, 417
BstMWI GCNNNNNNNGC 4 cut(s) 183, 382, 499, 522
BstNI CCWGG 1 cut(s) 320
BstPAI GACNNNNGTC 1 cut(s) 284
BstSCI CCNGG 1 cut(s) 318
BstSFI CTRYAG 1 cut(s) 44
BstUI CGCG 2 cut(s) 179, 529
BstV1I GCAGC 5 cut(s) 46, 58, 170, 286, 480
BstX2I RGATCY 2 cut(s) 14, 471
BstXI CCANNNNNNTGG 1 cut(s) 173
BstYI RGATCY 2 cut(s) 14, 471
BstZI CGGCCG 1 cut(s) 414
BsuRI GGCC 5 cut(s) 263, 295, 318, 402, 416
BtgI CCRYGG 1 cut(s) 214
BtsI GCAGTG 1 cut(s) 41
BtsIMutI CAGTG 1 cut(s) 41
Cac8I GCNNGC 7 cut(s) 64, 181, 370, 374, 483, 504, 527
CfoI GCGC 2 cut(s) 179, 378
Cfr13I GGNCC 5 cut(s) 4, 163, 293, 316, 401
Csp6I GTAC 2 cut(s) 243, 352
CviAII CATG 1 cut(s) 19
CviQI GTAC 2 cut(s) 243, 352
DpnI GATC 6 cut(s) 16, 84, 144, 206, 248, 473
DpnII GATC 6 cut(s) 14, 82, 142, 204, 246, 471
EaeI YGGCCR 1 cut(s) 414
EagI CGGCCG 1 cut(s) 414
EclXI CGGCCG 1 cut(s) 414
Eco147I AGGCCT 1 cut(s) 263
Eco47I GGWCC 2 cut(s) 4, 163
Eco52I CGGCCG 1 cut(s) 414
Eco57I CTGAAG 1 cut(s) 371
EcoRII CCWGG 1 cut(s) 318
FaeI CATG 1 cut(s) 22
FaiI YATR 4 cut(s) 20, 87, 221, 306
FatI CATG 1 cut(s) 18
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 8 cut(s) 47, 60, 148, 184, 275, 366, 414, 494
Fsp4HI GCNGC 8 cut(s) 47, 60, 148, 184, 275, 366, 414, 494
FspBI CTAG 2 cut(s) 63, 410
GlaI GCGC 2 cut(s) 178, 377
GluI GCNGC 8 cut(s) 47, 60, 148, 184, 275, 366, 414, 494
GsaI CCCAGC 2 cut(s) 500, 520
HaeIII GGCC 5 cut(s) 263, 295, 318, 402, 416
HapII CCGG 1 cut(s) 431
HhaI GCGC 2 cut(s) 179, 378
Hin1II CATG 1 cut(s) 22
Hin6I GCGC 2 cut(s) 177, 376
HinP1I GCGC 2 cut(s) 177, 376
HincII GTYRAC 2 cut(s) 346, 421
HindII GTYRAC 2 cut(s) 346, 421
HpaII CCGG 1 cut(s) 431
Hpy166II GTNNAC 3 cut(s) 346, 354, 421
Hpy188I TCNGA 2 cut(s) 130, 142
Hpy188III TCNNGA 3 cut(s) 202, 236, 431
Hpy8I GTNNAC 3 cut(s) 346, 354, 421
Hpy99I CGWCG 4 cut(s) 229, 260, 283, 347
HpyCH4III ACNGT 3 cut(s) 225, 242, 256
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 3 cut(s) 46, 186, 525
HpyF10VI GCNNNNNNNGC 4 cut(s) 183, 382, 499, 522
HpySE526I ACGT 1 cut(s) 342
Hsp92II CATG 1 cut(s) 22
HspAI GCGC 2 cut(s) 177, 376
Kpn2I TCCGGA 1 cut(s) 430
Kzo9I GATC 6 cut(s) 14, 82, 142, 204, 246, 471
LmnI GCTCC 1 cut(s) 490
Lsp1109I GCAGC 5 cut(s) 46, 58, 170, 286, 480
MaeI CTAG 2 cut(s) 63, 410
MaeII ACGT 1 cut(s) 342
MalI GATC 6 cut(s) 16, 84, 144, 206, 248, 473
MbiI CCGCTC 1 cut(s) 150
MboI GATC 6 cut(s) 14, 82, 142, 204, 246, 471
MboII GAAGA 1 cut(s) 190
MfeI CAATTG 1 cut(s) 30
MflI RGATCY 2 cut(s) 14, 471
MluCI AATT 3 cut(s) 9, 30, 450
MmeI TCCRAC 3 cut(s) 153, 363, 499
MnlI CCTC 9 cut(s) 49, 134, 143, 253, 274, 277, 367, 481, 505
MroI TCCGGA 1 cut(s) 430
MspA1I CMGCKG 1 cut(s) 372
MspI CCGG 1 cut(s) 431
MspR9I CCNGG 1 cut(s) 320
MunI CAATTG 1 cut(s) 30
MvaI CCWGG 1 cut(s) 320
MvnI CGCG 2 cut(s) 179, 529
MwoI GCNNNNNNNGC 4 cut(s) 183, 382, 499, 522
NdeII GATC 6 cut(s) 14, 82, 142, 204, 246, 471
NheI GCTAGC 1 cut(s) 62
NlaIII CATG 1 cut(s) 22
NlaIV GGNNCC 2 cut(s) 16, 294
NmeAIII GCCGAG 1 cut(s) 378
PceI AGGCCT 1 cut(s) 263
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 255
PflFI GACNNNGTC 1 cut(s) 167
PkrI GCNGC 8 cut(s) 48, 61, 149, 185, 276, 367, 415, 495
Ple19I CGATCG 2 cut(s) 145, 249
PshAI GACNNNNGTC 1 cut(s) 284
Psp6I CCWGG 1 cut(s) 318
PspFI CCCAGC 2 cut(s) 496, 516
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 16, 294
PspPI GGNCC 5 cut(s) 4, 163, 293, 316, 401
PstI CTGCAG 1 cut(s) 48
PsuI RGATCY 2 cut(s) 14, 471
PsyI GACNNNGTC 1 cut(s) 167
PvuI CGATCG 2 cut(s) 145, 249
PvuII CAGCTG 1 cut(s) 372
RsaI GTAC 2 cut(s) 244, 353
RsaNI GTAC 2 cut(s) 243, 352
SalI GTCGAC 1 cut(s) 344
SatI GCNGC 8 cut(s) 47, 60, 148, 184, 275, 366, 414, 494
Sau3AI GATC 6 cut(s) 14, 82, 142, 204, 246, 471
Sau96I GGNCC 5 cut(s) 4, 163, 293, 316, 401
ScrFI CCNGG 1 cut(s) 320
SfcI CTRYAG 1 cut(s) 44
SinI GGWCC 2 cut(s) 4, 163
Sse9I AATT 3 cut(s) 9, 30, 450
SseBI AGGCCT 1 cut(s) 263
SsiI CCGC 4 cut(s) 148, 366, 413, 529
SspMI CTAG 2 cut(s) 63, 410
StuI AGGCCT 1 cut(s) 263
StyD4I CCNGG 1 cut(s) 318
TaaI ACNGT 3 cut(s) 225, 242, 256
TaiI ACGT 1 cut(s) 345
TaqI TCGA 4 cut(s) 120, 237, 258, 345
TasI AATT 3 cut(s) 9, 30, 450
TatI WGTACW 1 cut(s) 351
TauI GCSGC 3 cut(s) 150, 368, 416
TscAI CASTG 1 cut(s) 48
TseI GCWGC 5 cut(s) 46, 59, 183, 274, 493
TspDTI ATGAA 1 cut(s) 462
TspGWI ACGGA 1 cut(s) 296
TspRI CASTG 1 cut(s) 48
Tth111I GACNNNGTC 1 cut(s) 167
VpaK11BI GGWCC 2 cut(s) 4, 163
XmiI GTMKAC 1 cut(s) 345
XspI CTAG 2 cut(s) 63, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.