Rorug04G0345100

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001116
Physical Location & Seq
Reverse (-)
51696269 .. 51702067
5799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0345100.1

Sequence Viewer

Length: 2313 bp
ATGGCCCCAACTCAGGAAAAAGCGACGCCTCCATGTTGCCCGAGCCCTCCACCTCCACCTCTATCCTCCAAACCCATTCTCCAGCTTCGAACCGCCTCCTCCGATCCGGCCCAGGATCCGATCTCAACCCGACCCTCCGTCCCGCCGCCCCTCATCCGACCCCTCGACACCACCTCAGCCAACACCGCTGCCGCCAAAGGCCTCCCAGTCATGTTGAGGGCTCAATCCAGCCATCCCTTGGAGCCTTTATCTGCCGCTGAAATCTCGGTAGCCGTGGCCACTGTCAGGGCAGCTGGAGCAACCCCTGAGGTGAGGGATAGTATGCGCTTTGTTGAAGTAGCTTTGGTAGAACCAGATAAGCGTGTTGTTGCATTGGCAGATGCGTATTTCTTCCCTCCTTTCCAGCCATCATTGCTTCCCAGAACAAAAGGAGGACCTATGATTCCTAGTAAACTTCCTCCGAGGCAAGCGAGGCTTGTAGTTTATAACAAAAAGTCAAATGAGACGAGTGTTTGGGTTGTTGAGCTATCGGAAGTTCATGCTGCAACTCGAGGTGGTCACCATAGGGGTAAAGTCATCTCATCCAAAGTTGTTCCAGATGTTCAGCCTCCCATGGATGCTATGGAATATGCTGAATGTGAAGCTGTTGTAAAGGACCATCCTCCATTTCGGGATGCAATGAAGAAGAGGGGTATTGAAGATTTGGACCTCGTAATGGTGGATCCATGGTGCGCAGGATATCACAGTGAGGCTGATGCTCCTAGCCGCAGGCTTGCTAAACCACTTATCTTCTGTAGAACTGAGAGTGATTGCCCTATGGAAAATGGTTATGCTCGTCCAGTGGAAGGAATCCATGTACTAGTTGATATGCAAAATATGGTTGTTCTGGAGTTTGAAGACCGTAAACTTGTTCCCCTGCCTCCTGCTGATCCTTTGAGAAATTATACTCCTGGTGAAACACGAGGAGGTGTTGATCGAAGTGATGTGAAACCCCTAAAGATAGTTCAGCCTGAAGGGCCAAGTTTTCGTGTTAATGGGCACTTTGTTGAATGGCAGAAGTGGAATTTCCGTATTGGCTTTACTTCGAGGGAAGGTTTGGTTATCTACTCTGCTGCTTATATTGACGGTAGTCGAGGCCGGAGGCCTGTGGCCCACAGGTTAAGTTTTGTTGAGATGGTGGTTCCTTATGGAGATCCAAATGATCCCCACTACAGGAAAAATGCATTTGATGCTGGGGAAGATGGGCTGGGTAAAAATGCACATTCTCTTAAAAAGGGTTGTGATTGTTTAGGTTATATCAAGTACTTTGATGCACACTTTACAAATTTCACTGGGGGTGTTGAAACAATTGAGAATTGTGTTTGCTTGCATGAGGAGGATCATGGAATATTATGGAAGCATCAGGATTGGAGAACAGGTTTAGCAGAAGTTCGACGGTCTAGAAGGCTGACGGTGTCTTTTATCTGTACAGTAGCTAACTATGAGTACGGATTTTACTGGCATTTTTATCAGGATGGGAAAATTGAAGCTGAGGTGAGACTTACAGGAATTCTAAGCTTAGGTGCGCTTCAACCAGGAGAAACCCGAAAGTATGGTACAACTATTGCACCTGGACTATATGCTCCTGTACATCAACACTTTTTTGTTGCTCGTATGGACATGGCAGTTGATAGTAAGCCTGGTGAAACTTTTAATCAGGTGGTTGAAGTAAATGTTAAAGTTGAAGAACCAGGAAAGAACAATGTTCATAACAATGCATTCTATGCTGAGGAGAAACTTCTGAAATCAGAATTGCAAGCAATGCGTGATTGTAATCCTTTATCTGCTCGCCATTGGATTGTTAGGCACACAAGAAATGTCAATCGGACAGGACAGCTAACAGGTTACAAGCTGGTACCTGGTTCAAATTGTTTACCATTAGCTGGTTCTGAGGCAAAGTTTCTGAGAAGAGCTGCTTTTTTGAAGCATAATCTTTGGGTCACGTCTTATGCACGTGATGAAATGTATCCTGGAGGAGAATTTCCTAATCAAAATCCACGTATTGGGGAGGGATTAGCCACTTGGGTTCAGCAAGATCGGTCTCTGGAAGAAGCTGACATTGTTCTTTGGTATGTATTTGGAGTGACCCACATTCCTCGACTGGAAGACTGGCCGGTCATGCCTGTGGAACATATTGGTTTTACGCTCATGCCACATGGTTTCTTTAACTGCTCAACGGCAGTGGACGTCCCTCCGAATACATGTGAGATGGACCTCAAGGAAAATGAGATGGCTGCAAAACCTATTCAGAATGGTCTTATAGCCAAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

770

Amino Acids

85.87

Weight (kDa)

6.69

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_amine_oxidN3 PF02728 203 - 306 2e-27 Copper amine oxidase, N3 domain
Cu_amine_oxid PF01179 332 - 739 4.2e-153 Copper amine oxidase, enzyme domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 486
AasI GACNNNNNNGTC 1 cut(s) 2153
AatII GACGTC 1 cut(s) 2229
Acc16I TGCGCA 1 cut(s) 733
Acc65I GGTACC 1 cut(s) 1894
AccB1I GGYRCC 1 cut(s) 1894
AccB7I CCANNNNNTGG 3 cut(s) 238, 1922, 2042
AciI CCGC 7 cut(s) 93, 143, 146, 186, 192, 255, 766
AclWI GGATC 9 cut(s) 98, 110, 123, 716, 729, 923, 1187, 1196, 1386
AcoI YGGCCR 2 cut(s) 276, 2150
AcsI RAATTY 4 cut(s) 1063, 1324, 1548, 2018
AcuI CTGAAG 1 cut(s) 1032
AcvI CACGTG 1 cut(s) 1994
AcyI GRCGYC 2 cut(s) 26, 2226
AfaI GTAC 7 cut(s) 858, 1304, 1468, 1487, 1597, 1629, 1896
AfiI CCNNNNNNNGG 8 cut(s) 13, 238, 285, 715, 845, 1212, 1922, 2042
AflIII ACRYGT 1 cut(s) 2240
AhdI GACNNNNNGTC 1 cut(s) 137
AhlI ACTAGT 1 cut(s) 859
AjiI CACGTC 1 cut(s) 1983
AjnI CCWGG 8 cut(s) 111, 949, 1573, 1609, 1678, 1729, 1897, 2008
Alw26I GTCTC 3 cut(s) 497, 1531, 2085
AlwI GGATC 9 cut(s) 98, 110, 123, 716, 729, 923, 1187, 1196, 1386
Ama87I CYCGRG 2 cut(s) 40, 549
AoxI GGCC 9 cut(s) 3, 108, 199, 276, 1016, 1135, 1142, 1149, 2150
ApeKI GCWGC 6 cut(s) 188, 290, 542, 1112, 1952, 2273
ApoI RAATTY 4 cut(s) 1063, 1324, 1548, 2018
Asp718I GGTACC 1 cut(s) 1894
AspLEI GCGC 3 cut(s) 327, 734, 1567
AspS9I GGNCC 8 cut(s) 4, 109, 434, 655, 706, 1016, 1150, 2251
AsuHPI GGTGA 5 cut(s) 322, 551, 965, 1546, 1694
AsuII TTCGAA 1 cut(s) 88
AvaI CYCGRG 2 cut(s) 40, 549
AvaII GGWCC 4 cut(s) 434, 655, 706, 2251
AxyI CCTNAGG 1 cut(s) 306
BaeGI GKGCMC 1 cut(s) 1041
BalI TGGCCA 1 cut(s) 278
BamHI GGATCC 2 cut(s) 115, 721
BanI GGYRCC 1 cut(s) 1894
BanII GRGCYC 2 cut(s) 47, 223
BauI CACGAG 1 cut(s) 960
BbrPI CACGTG 1 cut(s) 1994
BbsI GAAGAC 2 cut(s) 903, 2151
BbvCI CCTCAGC 3 cut(s) 175, 1530, 1767
BbvI GCAGC 6 cut(s) 175, 302, 529, 1099, 1939, 2260
BccI CCATC 8 cut(s) 240, 415, 666, 1168, 1235, 1508, 2242, 2263
BceAI ACGGC 2 cut(s) 257, 2232
BciT130I CCWGG 8 cut(s) 113, 951, 1575, 1611, 1680, 1731, 1899, 2010
BciVI GTATCC 1 cut(s) 2016
BcoDI GTCTC 3 cut(s) 497, 1531, 2085
BcuI ACTAGT 1 cut(s) 859
BfaI CTAG 4 cut(s) 447, 762, 860, 1440
BfmI CTRYAG 2 cut(s) 793, 1210
BfuI GTATCC 1 cut(s) 2016
BglI GCCNNNNNGGC 1 cut(s) 1015
BmcAI AGTACT 1 cut(s) 1304
Bme1390I CCNGG 8 cut(s) 113, 951, 1575, 1611, 1680, 1731, 1899, 2010
Bme18I GGWCC 4 cut(s) 434, 655, 706, 2251
BmeRI GACNNNNNGTC 1 cut(s) 137
BmeT110I CYCGRG 2 cut(s) 40, 549
BmgBI CACGTC 1 cut(s) 1983
BmgT120I GGNCC 8 cut(s) 4, 109, 434, 655, 706, 1016, 1150, 2251
BmiI GGNNCC 6 cut(s) 6, 117, 243, 723, 1182, 1896
BmrFI CCNGG 8 cut(s) 113, 951, 1575, 1611, 1680, 1731, 1899, 2010
BmrI ACTGGG 2 cut(s) 200, 1341
BmsI GCATC 7 cut(s) 370, 607, 664, 745, 1219, 1300, 1408
BmuI ACTGGG 2 cut(s) 200, 1341
BoxI GACNNNNGTC 1 cut(s) 1128
BpiI GAAGAC 2 cut(s) 903, 2151
BpmI CTGGAG 4 cut(s) 65, 315, 908, 2031
Bpu10I CCTNAGC 4 cut(s) 175, 1530, 1558, 1767
Bpu14I TTCGAA 1 cut(s) 88
BpuEI CTTGAG 1 cut(s) 2240
BsaAI YACGTR 2 cut(s) 1994, 2039
BsaBI GATNNNNATC 1 cut(s) 1812
BsaHI GRCGYC 2 cut(s) 26, 2226
BsaI GGTCTC 1 cut(s) 2085
BsaJI CCNNGG 6 cut(s) 111, 237, 273, 461, 612, 725
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 1402, 1432
Bsc4I CCNNNNNNNGG 8 cut(s) 13, 238, 285, 715, 845, 1212, 1922, 2042
Bse118I RCCGGY 1 cut(s) 2152
Bse1I ACTGG 6 cut(s) 206, 839, 1336, 1502, 2145, 2153
Bse21I CCTNAGG 1 cut(s) 306
Bse3DI GCAATG 3 cut(s) 410, 684, 1806
Bse8I GATNNNNATC 1 cut(s) 1812
BseBI CCWGG 8 cut(s) 113, 951, 1575, 1611, 1680, 1731, 1899, 2010
BseDI CCNNGG 6 cut(s) 111, 237, 273, 461, 612, 725
BseGI GGATG 7 cut(s) 153, 232, 581, 622, 658, 679, 1519
BseJI GATNNNNATC 1 cut(s) 1812
BseLI CCNNNNNNNGG 8 cut(s) 13, 238, 285, 715, 845, 1212, 1922, 2042
BseMI GCAATG 3 cut(s) 410, 684, 1806
BseMII CTCAG 8 cut(s) 26, 189, 297, 792, 1521, 1758, 1920, 1934
BseNI ACTGG 6 cut(s) 206, 839, 1336, 1502, 2145, 2153
BseRI GAGGAG 5 cut(s) 88, 978, 1388, 1784, 2028
BseSI GKGCMC 1 cut(s) 1041
BseXI GCAGC 6 cut(s) 175, 302, 529, 1099, 1939, 2260
BseYI CCCAGC 2 cut(s) 1232, 1246
BshFI GGCC 9 cut(s) 5, 110, 201, 278, 1018, 1137, 1144, 1151, 2152
BshNI GGYRCC 1 cut(s) 1894
BsiHKCI CYCGRG 2 cut(s) 40, 549
BsiSI CCGG 3 cut(s) 107, 1138, 2153
BslFI GGGAC 2 cut(s) 125, 2213
BslI CCNNNNNNNGG 8 cut(s) 13, 238, 285, 715, 845, 1212, 1922, 2042
BsmAI GTCTC 3 cut(s) 497, 1531, 2085
BsmBI CGTCTC 1 cut(s) 497
BsmFI GGGAC 2 cut(s) 125, 2213
BsmI GAATGC 1 cut(s) 1757
BsnI GGCC 9 cut(s) 5, 110, 201, 278, 1018, 1137, 1144, 1151, 2152
Bso31I GGTCTC 1 cut(s) 2085
BsoBI CYCGRG 2 cut(s) 40, 549
Bsp119I TTCGAA 1 cut(s) 88
Bsp1286I GDGCHC 3 cut(s) 47, 223, 1041
Bsp1407I TGTACA 2 cut(s) 1466, 1627
Bsp19I CCATGG 2 cut(s) 612, 725
BspACI CCGC 7 cut(s) 93, 143, 146, 186, 192, 255, 766
BspANI GGCC 9 cut(s) 5, 110, 201, 278, 1018, 1137, 1144, 1151, 2152
BspCNI CTCAG 8 cut(s) 25, 188, 298, 793, 1522, 1759, 1921, 1935
BspLI GGNNCC 6 cut(s) 6, 117, 243, 723, 1182, 1896
BspPI GGATC 9 cut(s) 98, 110, 123, 716, 729, 923, 1187, 1196, 1386
BspQI GCTCTTC 1 cut(s) 1942
BspT104I TTCGAA 1 cut(s) 88
BspT107I GGYRCC 1 cut(s) 1894
BspTNI GGTCTC 1 cut(s) 2085
BsrDI GCAATG 3 cut(s) 410, 684, 1806
BsrFI RCCGGY 1 cut(s) 2152
BsrGI TGTACA 2 cut(s) 1466, 1627
BsrI ACTGG 6 cut(s) 206, 839, 1336, 1502, 2145, 2153
BssAI RCCGGY 1 cut(s) 2152
BssECI CCNNGG 6 cut(s) 111, 237, 273, 461, 612, 725
BssNI GRCGYC 2 cut(s) 26, 2226
BssSI CACGAG 1 cut(s) 960
BssT1I CCWWGG 3 cut(s) 237, 612, 725
Bst2BI CACGAG 1 cut(s) 960
Bst2UI CCWGG 8 cut(s) 113, 951, 1575, 1611, 1680, 1731, 1899, 2010
Bst4CI ACNGT 7 cut(s) 283, 746, 902, 1127, 1437, 1453, 1471
Bst6I CTCTTC 2 cut(s) 680, 1942
BstACI GRCGYC 2 cut(s) 26, 2226
BstAPI GCANNNNNTGC 3 cut(s) 1229, 1763, 1801
BstAUI TGTACA 2 cut(s) 1466, 1627
BstBAI YACGTR 2 cut(s) 1994, 2039
BstBI TTCGAA 1 cut(s) 88
BstC8I GCNNGC 6 cut(s) 468, 770, 774, 1367, 1797, 1828
BstDSI CCRYGG 3 cut(s) 273, 612, 725
BstEII GGTNACC 1 cut(s) 557
BstF5I GGATG 7 cut(s) 153, 232, 581, 622, 658, 679, 1519
BstHHI GCGC 3 cut(s) 327, 734, 1567
BstMAI GTCTC 3 cut(s) 497, 1531, 2085
BstMWI GCNNNNNNNGC 9 cut(s) 185, 296, 412, 472, 1015, 1229, 1763, 1801, 2158
BstNI CCWGG 8 cut(s) 113, 951, 1575, 1611, 1680, 1731, 1899, 2010
BstNSI RCATGY 1 cut(s) 2244
BstPAI GACNNNNGTC 1 cut(s) 1128
BstPI GGTNACC 1 cut(s) 557
BstSCI CCNGG 8 cut(s) 111, 949, 1573, 1609, 1678, 1729, 1897, 2008
BstSFI CTRYAG 2 cut(s) 793, 1210
BstSLI GKGCMC 1 cut(s) 1041
BstV1I GCAGC 6 cut(s) 175, 302, 529, 1099, 1939, 2260
BstV2I GAAGAC 2 cut(s) 903, 2151
BstX2I RGATCY 3 cut(s) 115, 721, 1192
BstYI RGATCY 3 cut(s) 115, 721, 1192
Bsu36I CCTNAGG 1 cut(s) 306
BsuI GTATCC 1 cut(s) 2016
BsuRI GGCC 9 cut(s) 5, 110, 201, 278, 1018, 1137, 1144, 1151, 2152
BtgI CCRYGG 3 cut(s) 273, 612, 725
BtrI CACGTC 1 cut(s) 1983
BtsCI GGATG 7 cut(s) 153, 232, 581, 622, 658, 679, 1519
BtsI GCAGTG 1 cut(s) 2226
BtsIMutI CAGTG 5 cut(s) 279, 751, 846, 1329, 2226
Cac8I GCNNGC 6 cut(s) 468, 770, 774, 1367, 1797, 1828
CfoI GCGC 3 cut(s) 327, 734, 1567
Cfr10I RCCGGY 1 cut(s) 2152
Cfr13I GGNCC 8 cut(s) 4, 109, 434, 655, 706, 1016, 1150, 2251
CseI GACGC 1 cut(s) 34
CsiI ACCWGGT 1 cut(s) 1897
Csp6I GTAC 7 cut(s) 857, 1303, 1467, 1486, 1596, 1628, 1895
CspCI CAANNNNNGTGG 2 cut(s) 2202, 2237
CviQI GTAC 7 cut(s) 857, 1303, 1467, 1486, 1596, 1628, 1895
DrdI GACNNNNNNGTC 1 cut(s) 2153
DriI GACNNNNNGTC 1 cut(s) 137
DseDI GACNNNNNNGTC 1 cut(s) 2153
EaeI YGGCCR 2 cut(s) 276, 2150
Eam1104I CTCTTC 2 cut(s) 680, 1942
Eam1105I GACNNNNNGTC 1 cut(s) 137
EarI CTCTTC 2 cut(s) 680, 1942
Eco130I CCWWGG 3 cut(s) 237, 612, 725
Eco147I AGGCCT 2 cut(s) 201, 1144
Eco24I GRGCYC 2 cut(s) 47, 223
Eco31I GGTCTC 1 cut(s) 2085
Eco32I GATATC 1 cut(s) 740
Eco47I GGWCC 4 cut(s) 434, 655, 706, 2251
Eco57I CTGAAG 1 cut(s) 1032
Eco72I CACGTG 1 cut(s) 1994
Eco81I CCTNAGG 1 cut(s) 306
Eco88I CYCGRG 2 cut(s) 40, 549
Eco91I GGTNACC 1 cut(s) 557
EcoO109I RGGNCCY 1 cut(s) 434
EcoO65I GGTNACC 1 cut(s) 557
EcoRI GAATTC 1 cut(s) 1548
EcoRII CCWGG 8 cut(s) 111, 949, 1573, 1609, 1678, 1729, 1897, 2008
EcoRV GATATC 1 cut(s) 740
EcoT14I CCWWGG 3 cut(s) 237, 612, 725
EcoT22I ATGCAT 2 cut(s) 1225, 1759
EcoT38I GRGCYC 2 cut(s) 47, 223
ErhI CCWWGG 3 cut(s) 237, 612, 725
Esp3I CGTCTC 1 cut(s) 497
FalI AAGNNNNNCTT 4 cut(s) 459, 491, 1939, 1971
FaqI GGGAC 2 cut(s) 125, 2213
FauI CCCGC 1 cut(s) 150
FokI GGATG 7 cut(s) 140, 219, 568, 629, 645, 686, 1526
FriOI GRGCYC 2 cut(s) 47, 223
FspBI CTAG 4 cut(s) 447, 762, 860, 1440
FspI TGCGCA 1 cut(s) 733
GlaI GCGC 3 cut(s) 326, 733, 1566
GsaI CCCAGC 2 cut(s) 1236, 1250
GsuI CTGGAG 4 cut(s) 65, 315, 908, 2031
HaeIII GGCC 9 cut(s) 5, 110, 201, 278, 1018, 1137, 1144, 1151, 2152
HapII CCGG 3 cut(s) 107, 1138, 2153
HgaI GACGC 1 cut(s) 34
HhaI GCGC 3 cut(s) 327, 734, 1567
Hin1I GRCGYC 2 cut(s) 26, 2226
Hin6I GCGC 3 cut(s) 325, 732, 1565
HinP1I GCGC 3 cut(s) 325, 732, 1565
HindIII AAGCTT 1 cut(s) 1555
HinfI GANTC 2 cut(s) 442, 849
HpaII CCGG 3 cut(s) 107, 1138, 2153
HphI GGTGA 5 cut(s) 322, 551, 965, 1546, 1694
Hpy166II GTNNAC 4 cut(s) 452, 905, 1913, 2224
Hpy188III TCNNGA 8 cut(s) 14, 596, 671, 887, 1403, 1440, 1511, 2084
Hpy8I GTNNAC 4 cut(s) 452, 905, 1913, 2224
Hpy99I CGWCG 2 cut(s) 28, 1437
HpyAV CCTTC 4 cut(s) 839, 1007, 1085, 1437
HpyCH4III ACNGT 7 cut(s) 283, 746, 902, 1127, 1437, 1453, 1471
HpyCH4IV ACGT 4 cut(s) 1982, 1993, 2038, 2226
HpyF10VI GCNNNNNNNGC 9 cut(s) 185, 296, 412, 472, 1015, 1229, 1763, 1801, 2158
HpySE526I ACGT 4 cut(s) 1982, 1993, 2038, 2226
Hsp92I GRCGYC 2 cut(s) 26, 2226
HspAI GCGC 3 cut(s) 325, 732, 1565
KpnI GGTACC 1 cut(s) 1898
LguI GCTCTTC 1 cut(s) 1942
LmnI GCTCC 4 cut(s) 241, 296, 763, 1627
Lsp1109I GCAGC 6 cut(s) 175, 302, 529, 1099, 1939, 2260
LweI GCATC 7 cut(s) 370, 607, 664, 745, 1219, 1300, 1408
MabI ACCWGGT 1 cut(s) 1897
MaeI CTAG 4 cut(s) 447, 762, 860, 1440
MaeII ACGT 4 cut(s) 1982, 1993, 2038, 2226
MaeIII GTNAC 4 cut(s) 557, 1883, 1978, 2122
MfeI CAATTG 1 cut(s) 1347
MflI RGATCY 3 cut(s) 115, 721, 1192
MhlI GDGCHC 3 cut(s) 47, 223, 1041
MlsI TGGCCA 1 cut(s) 278
MluNI TGGCCA 1 cut(s) 278
MmeI TCCRAC 1 cut(s) 181
Mox20I TGGCCA 1 cut(s) 278
Mph1103I ATGCAT 2 cut(s) 1225, 1759
MscI TGGCCA 1 cut(s) 278
MseI TTAA 6 cut(s) 1032, 1160, 1269, 1692, 1716, 2205
MslI CAYNNNNRTG 2 cut(s) 1752, 2162
Msp20I TGGCCA 1 cut(s) 278
MspA1I CMGCKG 3 cut(s) 188, 257, 293
MspI CCGG 3 cut(s) 107, 1138, 2153
MspR9I CCNGG 8 cut(s) 113, 951, 1575, 1611, 1680, 1731, 1899, 2010
MunI CAATTG 1 cut(s) 1347
Mva1269I GAATGC 1 cut(s) 1757
MvaI CCWGG 8 cut(s) 113, 951, 1575, 1611, 1680, 1731, 1899, 2010
MwoI GCNNNNNNNGC 9 cut(s) 185, 296, 412, 472, 1015, 1229, 1763, 1801, 2158
NcoI CCATGG 2 cut(s) 612, 725
NlaIV GGNNCC 6 cut(s) 6, 117, 243, 723, 1182, 1896
NmuCI GTSAC 3 cut(s) 557, 1978, 2122
NsbI TGCGCA 1 cut(s) 733
NsiI ATGCAT 2 cut(s) 1225, 1759
NspI RCATGY 1 cut(s) 2244
NspV TTCGAA 1 cut(s) 88
PaeR7I CTCGAG 1 cut(s) 549
PceI AGGCCT 2 cut(s) 201, 1144
PciI ACATGT 1 cut(s) 2240
PciSI GCTCTTC 1 cut(s) 1942
PctI GAATGC 1 cut(s) 1757
PfeI GAWTC 2 cut(s) 442, 849
PflFI GACNNNGTC 1 cut(s) 1453
PflMI CCANNNNNTGG 3 cut(s) 238, 1922, 2042
PfoI TCCNGGA 1 cut(s) 2008
PmaCI CACGTG 1 cut(s) 1994
PmlI CACGTG 1 cut(s) 1994
Ppu21I YACGTR 2 cut(s) 1994, 2039
PpuMI RGGWCCY 1 cut(s) 434
PscI ACATGT 1 cut(s) 2240
PshAI GACNNNNGTC 1 cut(s) 1128
PsiI TTATAA 1 cut(s) 486
Psp5II RGGWCCY 1 cut(s) 434
Psp6I CCWGG 8 cut(s) 111, 949, 1573, 1609, 1678, 1729, 1897, 2008
PspCI CACGTG 1 cut(s) 1994
PspEI GGTNACC 1 cut(s) 557
PspFI CCCAGC 2 cut(s) 1232, 1246
PspGI CCWGG 8 cut(s) 111, 949, 1573, 1609, 1678, 1729, 1897, 2008
PspN4I GGNNCC 6 cut(s) 6, 117, 243, 723, 1182, 1896
PspPI GGNCC 8 cut(s) 4, 109, 434, 655, 706, 1016, 1150, 2251
PspPPI RGGWCCY 1 cut(s) 434
PspXI VCTCGAGB 1 cut(s) 549
PsuI RGATCY 3 cut(s) 115, 721, 1192
PsyI GACNNNGTC 1 cut(s) 1453
PvuII CAGCTG 1 cut(s) 293
RsaI GTAC 7 cut(s) 858, 1304, 1468, 1487, 1597, 1629, 1896
RsaNI GTAC 7 cut(s) 857, 1303, 1467, 1486, 1596, 1628, 1895
RseI CAYNNNNRTG 2 cut(s) 1752, 2162
SapI GCTCTTC 1 cut(s) 1942
SaqAI TTAA 6 cut(s) 1032, 1160, 1269, 1692, 1716, 2205
Sau96I GGNCC 8 cut(s) 4, 109, 434, 655, 706, 1016, 1150, 2251
ScaI AGTACT 1 cut(s) 1304
ScrFI CCNGG 8 cut(s) 113, 951, 1575, 1611, 1680, 1731, 1899, 2010
SduI GDGCHC 3 cut(s) 47, 223, 1041
SexAI ACCWGGT 1 cut(s) 1897
SfaNI GCATC 7 cut(s) 370, 607, 664, 745, 1219, 1300, 1408
SfcI CTRYAG 2 cut(s) 793, 1210
Sfr274I CTCGAG 1 cut(s) 549
SfuI TTCGAA 1 cut(s) 88
SinI GGWCC 4 cut(s) 434, 655, 706, 2251
SlaI CTCGAG 1 cut(s) 549
SmiMI CAYNNNNRTG 2 cut(s) 1752, 2162
SmlI CTYRAG 2 cut(s) 549, 2255
SmoI CTYRAG 2 cut(s) 549, 2255
SpeI ACTAGT 1 cut(s) 859
SseBI AGGCCT 2 cut(s) 201, 1144
SsiI CCGC 7 cut(s) 93, 143, 146, 186, 192, 255, 766
SspI AATATT 1 cut(s) 1389
SspMI CTAG 4 cut(s) 447, 762, 860, 1440
StuI AGGCCT 2 cut(s) 201, 1144
StyD4I CCNGG 8 cut(s) 111, 949, 1573, 1609, 1678, 1729, 1897, 2008
StyI CCWWGG 3 cut(s) 237, 612, 725
TaaI ACNGT 7 cut(s) 283, 746, 902, 1127, 1437, 1453, 1471
TaiI ACGT 4 cut(s) 1985, 1996, 2041, 2229
TaqI TCGA 8 cut(s) 88, 165, 550, 976, 1085, 1132, 1432, 2137
TaqII GACCGA 1 cut(s) 2067
TatI WGTACW 4 cut(s) 856, 1302, 1466, 1627
TauI GCSGC 4 cut(s) 148, 194, 257, 768
TfiI GAWTC 2 cut(s) 442, 849
Tru1I TTAA 6 cut(s) 1032, 1160, 1269, 1692, 1716, 2205
Tru9I TTAA 6 cut(s) 1032, 1160, 1269, 1692, 1716, 2205
TscAI CASTG 5 cut(s) 286, 751, 846, 1336, 2226
TseFI GTSAC 3 cut(s) 557, 1978, 2122
TseI GCWGC 6 cut(s) 188, 290, 542, 1112, 1952, 2273
Tsp45I GTSAC 3 cut(s) 557, 1978, 2122
TspDTI ATGAA 4 cut(s) 527, 695, 1736, 2013
TspGWI ACGGA 3 cut(s) 127, 1058, 1503
TspRI CASTG 5 cut(s) 286, 751, 846, 1336, 2226
Tth111I GACNNNGTC 1 cut(s) 1453
Van91I CCANNNNNTGG 3 cut(s) 238, 1922, 2042
VpaK11BI GGWCC 4 cut(s) 434, 655, 706, 2251
XapI RAATTY 4 cut(s) 1063, 1324, 1548, 2018
XbaI TCTAGA 1 cut(s) 1439
XceI RCATGY 1 cut(s) 2244
XcmI CCANNNNNNNNNTGG 2 cut(s) 235, 619
XhoI CTCGAG 1 cut(s) 549
XspI CTAG 4 cut(s) 447, 762, 860, 1440
ZraI GACGTC 1 cut(s) 2227
ZrmI AGTACT 1 cut(s) 1304
Zsp2I ATGCAT 2 cut(s) 1225, 1759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.