RchiOBHm_Chr1g0327761

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001116
Physical Location & Seq
Forward (+)
16693438 .. 16694602
1165 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55725

Sequence Viewer

Length: 1086 bp
ATGGTGGCAAATATTGGCGAACAGGGTTATGATAGAGCTGAAGAAGTGAAGCAATTCGACGACTCCAAGCTTGGAGTCAAAGGCCTGGTCGACTCGGGTCTCTCCTCCATCCCTCAAATCTTTATCCACCCGCCCGAAACATTACAACAGTTGCAGTCCAAGACCCGACCCGACGCCCAACCAATCCCCACCATCGACCTCTCCGACCTCCACTCTGATCGCCGCTTTGCCATAGTCGACCAAGTCAGCGCCTTGTGCCGCAACTTTGGTTTCTTCCAGATCACCAACCATGGCATACCTTCGGTGGTAATGGACCGTACGATCGCCGCCATCAAGGTCTTCCATGAGCAGCCGACGGAGGTTAAAGCGCAATTCTACCGTAGAGGGAGGGGCCTAGGTGTGTCATATCTCTCCAACGTCGACCTGTACAAGGCCAAAGCTGCCAGCTGGCGCGACACGCTTCAGGTGAGGCTCGGCCCTGTGCTGGCGGTCGCCGATGTTCCGGAGGTTTGTAGGGACGAAATTGTTGCATGGAACCGAGAGATCCAACGGTTGGGAGAGGACTTGCTGGGGTTGATGAGTGAAGGACTGGGGTTGCGCACGGAGAAGCTGAAGGACATGACGTGTCTGAAAGGAAGGGTGATGTTGGGCCACTACTACCCGCCCTGCCCACAGCCTGATCTAACAGTTGGGCTTACTTCACATAGTGACCCGGGAGTGTTGACCATCCTGCTTCAAGATCATATTGGTGGGTTGCAGGTTAAGCACGATGGGGCTTGGGTGGATGTGAAACCTATCCCCGGTGCTCTTGTGATTAATGTTGGTGATTTACTTCAGATTATTTCAAATGAGGAATACAAAAGCGTGGATCACCGGGTGTTGGCGAATCCACACCACGAGCCTCGTGTCTCAGTAGCCGTGTTTTTGAACCCGAGCAATAGAGAGGGGTTGTTTGGTCCGCTGCCAGAGTTGGTCTCTCCGGAGAAGCCAGCTCTCTACAAAGAGTTTAAGTTTTCGGAATTCATGACAAGGGTATTCAGCAAAGAATTGAGTGGCAAATCCTTAGTAAATTATTCTAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

361

Amino Acids

40.17

Weight (kDa)

6.27

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 62 - 156 3.5e-18 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 214 - 311 2e-33 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 599
Acc36I ACCTGC 1 cut(s) 748
AccB7I CCANNNNNTGG 1 cut(s) 553
AccI GTMKAC 3 cut(s) 90, 237, 420
AccII CGCG 1 cut(s) 453
AccIII TCCGGA 2 cut(s) 502, 979
AciI CCGC 7 cut(s) 131, 223, 259, 327, 488, 662, 959
AclWI GGATC 2 cut(s) 538, 876
AcsI RAATTY 1 cut(s) 1019
AcuI CTGAAG 4 cut(s) 60, 446, 632, 818
AcyI GRCGYC 1 cut(s) 174
AdeI CACNNNGTG 2 cut(s) 707, 877
AfaI GTAC 2 cut(s) 319, 428
AfiI CCNNNNNNNGG 5 cut(s) 430, 484, 553, 800, 880
AflIII ACRYGT 1 cut(s) 623
AgsI TTSAA 3 cut(s) 737, 846, 928
AjiI CACGTC 1 cut(s) 624
AjnI CCWGG 1 cut(s) 84
AluBI AGCT 6 cut(s) 38, 70, 440, 447, 610, 992
AluI AGCT 6 cut(s) 38, 70, 440, 447, 610, 992
Alw21I GWGCWC 1 cut(s) 808
Alw26I GTCTC 3 cut(s) 104, 913, 979
AlwI GGATC 2 cut(s) 538, 876
Ama87I CYCGRG 3 cut(s) 94, 712, 931
Aor13HI TCCGGA 2 cut(s) 502, 979
AoxI GGCC 5 cut(s) 82, 391, 432, 475, 649
ApeKI GCWGC 3 cut(s) 349, 440, 961
ApoI RAATTY 1 cut(s) 1019
ArsI GACNNNNNNTTYG 2 cut(s) 72, 104
AseI ATTAAT 1 cut(s) 816
Asp700I GAANNNNTTC 1 cut(s) 53
AspA2I CCTAGG 1 cut(s) 394
AspLEI GCGC 4 cut(s) 251, 370, 453, 600
AspS9I GGNCC 5 cut(s) 313, 391, 476, 649, 956
AsuC2I CCSGG 4 cut(s) 713, 714, 801, 875
AsuHPI GGTGA 5 cut(s) 274, 478, 652, 836, 863
AvaI CYCGRG 3 cut(s) 94, 712, 931
AvaII GGWCC 2 cut(s) 313, 956
AvrII CCTAGG 1 cut(s) 394
BauI CACGAG 2 cut(s) 896, 903
BbsI GAAGAC 1 cut(s) 331
Bbv12I GWGCWC 1 cut(s) 808
BbvI GCAGC 3 cut(s) 361, 427, 948
BccI CCATC 5 cut(s) 116, 200, 338, 734, 764
BceAI ACGGC 1 cut(s) 902
BcgI CGANNNNNNTGC 2 cut(s) 509, 543
BciT130I CCWGG 1 cut(s) 86
BcnI CCSGG 4 cut(s) 713, 714, 801, 875
BcoDI GTCTC 3 cut(s) 104, 913, 979
BfaI CTAG 1 cut(s) 395
BfoI RGCGCY 1 cut(s) 252
BfuAI ACCTGC 1 cut(s) 748
BisI GCNGC 6 cut(s) 223, 259, 327, 350, 441, 962
BlnI CCTAGG 1 cut(s) 394
BlsI GCNGC 6 cut(s) 224, 260, 328, 351, 442, 963
Bme1390I CCNGG 5 cut(s) 86, 713, 714, 801, 875
Bme18I GGWCC 2 cut(s) 313, 956
BmeT110I CYCGRG 3 cut(s) 94, 712, 931
BmgBI CACGTC 1 cut(s) 624
BmgT120I GGNCC 5 cut(s) 313, 391, 476, 649, 956
BmiI GGNNCC 2 cut(s) 392, 536
BmrFI CCNGG 5 cut(s) 86, 713, 714, 801, 875
BmrI ACTGGG 1 cut(s) 599
BmuI ACTGGG 1 cut(s) 599
BoxI GACNNNNGTC 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 331
BplI GAGNNNNNCTC 2 cut(s) 959, 991
BpuMI CCSGG 4 cut(s) 713, 714, 801, 875
BsaHI GRCGYC 1 cut(s) 174
BsaI GGTCTC 2 cut(s) 104, 979
BsaJI CCNNGG 4 cut(s) 289, 394, 712, 799
BsaWI WCCGGW 2 cut(s) 502, 979
Bsc4I CCNNNNNNNGG 5 cut(s) 430, 484, 553, 800, 880
Bse1I ACTGG 1 cut(s) 594
BseAI TCCGGA 2 cut(s) 502, 979
BseBI CCWGG 1 cut(s) 86
BseDI CCNNGG 4 cut(s) 289, 394, 712, 799
BseGI GGATG 3 cut(s) 108, 726, 790
BseLI CCNNNNNNNGG 5 cut(s) 430, 484, 553, 800, 880
BseMII CTCAG 1 cut(s) 924
BseNI ACTGG 1 cut(s) 594
BseRI GAGGAG 1 cut(s) 94
BseXI GCAGC 3 cut(s) 361, 427, 948
BseYI CCCAGC 1 cut(s) 568
Bsh1236I CGCG 1 cut(s) 453
Bsh1285I CGRYCG 2 cut(s) 324, 492
BshFI GGCC 5 cut(s) 84, 393, 434, 477, 651
BsiEI CGRYCG 2 cut(s) 324, 492
BsiHKAI GWGCWC 1 cut(s) 808
BsiHKCI CYCGRG 3 cut(s) 94, 712, 931
BsiSI CCGG 5 cut(s) 503, 713, 801, 874, 980
BsiWI CGTACG 1 cut(s) 317
BslFI GGGAC 1 cut(s) 530
BslI CCNNNNNNNGG 5 cut(s) 430, 484, 553, 800, 880
BsmAI GTCTC 3 cut(s) 104, 913, 979
BsmFI GGGAC 1 cut(s) 530
BsnI GGCC 5 cut(s) 84, 393, 434, 477, 651
Bso31I GGTCTC 2 cut(s) 104, 979
BsoBI CYCGRG 3 cut(s) 94, 712, 931
Bsp1286I GDGCHC 1 cut(s) 808
Bsp13I TCCGGA 2 cut(s) 502, 979
Bsp1407I TGTACA 1 cut(s) 426
Bsp143I GATC 7 cut(s) 217, 279, 321, 543, 679, 739, 868
Bsp19I CCATGG 1 cut(s) 289
BspACI CCGC 7 cut(s) 131, 223, 259, 327, 488, 662, 959
BspANI GGCC 5 cut(s) 84, 393, 434, 477, 651
BspCNI CTCAG 1 cut(s) 923
BspEI TCCGGA 2 cut(s) 502, 979
BspFNI CGCG 1 cut(s) 453
BspHI TCATGA 1 cut(s) 1023
BspLI GGNNCC 2 cut(s) 392, 536
BspMI ACCTGC 1 cut(s) 748
BspPI GGATC 2 cut(s) 538, 876
BspTNI GGTCTC 2 cut(s) 104, 979
BsrGI TGTACA 1 cut(s) 426
BsrI ACTGG 1 cut(s) 594
BssECI CCNNGG 4 cut(s) 289, 394, 712, 799
BssMI GATC 7 cut(s) 217, 279, 321, 543, 679, 739, 868
BssNI GRCGYC 1 cut(s) 174
BssSI CACGAG 2 cut(s) 896, 903
BssT1I CCWWGG 2 cut(s) 289, 394
Bst2BI CACGAG 2 cut(s) 896, 903
Bst2UI CCWGG 1 cut(s) 86
Bst4CI ACNGT 5 cut(s) 150, 317, 380, 552, 688
BstACI GRCGYC 1 cut(s) 174
BstAUI TGTACA 1 cut(s) 426
BstC8I GCNNGC 4 cut(s) 445, 449, 486, 990
BstDEI CTNAG 3 cut(s) 910, 1063, 1077
BstDSI CCRYGG 1 cut(s) 289
BstENI CCTNNNNNAGG 1 cut(s) 428
BstF5I GGATG 3 cut(s) 108, 726, 790
BstFNI CGCG 1 cut(s) 453
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 4 cut(s) 251, 370, 453, 600
BstKTI GATC 7 cut(s) 220, 282, 324, 546, 682, 742, 871
BstMAI GTCTC 3 cut(s) 104, 913, 979
BstMBI GATC 7 cut(s) 217, 279, 321, 543, 679, 739, 868
BstMCI CGRYCG 2 cut(s) 324, 492
BstMWI GCNNNNNNNGC 4 cut(s) 255, 440, 457, 763
BstNI CCWGG 1 cut(s) 86
BstPAI GACNNNNGTC 1 cut(s) 96
BstSCI CCNGG 5 cut(s) 84, 711, 712, 799, 873
BstUI CGCG 1 cut(s) 453
BstV1I GCAGC 3 cut(s) 361, 427, 948
BstV2I GAAGAC 1 cut(s) 331
BstX2I RGATCY 1 cut(s) 543
BstYI RGATCY 1 cut(s) 543
BsuRI GGCC 5 cut(s) 84, 393, 434, 477, 651
BtgI CCRYGG 1 cut(s) 289
BtrI CACGTC 1 cut(s) 624
BtsCI GGATG 3 cut(s) 108, 726, 790
BveI ACCTGC 1 cut(s) 748
Cac8I GCNNGC 4 cut(s) 445, 449, 486, 990
CciI TCATGA 1 cut(s) 1023
CfoI GCGC 4 cut(s) 251, 370, 453, 600
Cfr13I GGNCC 5 cut(s) 313, 391, 476, 649, 956
Cfr9I CCCGGG 1 cut(s) 712
CseI GACGC 1 cut(s) 182
Csp6I GTAC 2 cut(s) 318, 427
CviAII CATG 5 cut(s) 290, 344, 531, 619, 1024
CviQI GTAC 2 cut(s) 318, 427
DdeI CTNAG 3 cut(s) 910, 1063, 1077
DpnI GATC 7 cut(s) 219, 281, 323, 545, 681, 741, 870
DpnII GATC 7 cut(s) 217, 279, 321, 543, 679, 739, 868
DraIII CACNNNGTG 2 cut(s) 707, 877
Eco130I CCWWGG 2 cut(s) 289, 394
Eco147I AGGCCT 1 cut(s) 84
Eco31I GGTCTC 2 cut(s) 104, 979
Eco47I GGWCC 2 cut(s) 313, 956
Eco57I CTGAAG 4 cut(s) 60, 446, 632, 818
Eco88I CYCGRG 3 cut(s) 94, 712, 931
EcoNI CCTNNNNNAGG 1 cut(s) 428
EcoO109I RGGNCCY 1 cut(s) 391
EcoRI GAATTC 1 cut(s) 1019
EcoRII CCWGG 1 cut(s) 84
EcoT14I CCWWGG 2 cut(s) 289, 394
ErhI CCWWGG 2 cut(s) 289, 394
FaeI CATG 5 cut(s) 293, 347, 534, 622, 1027
FaqI GGGAC 1 cut(s) 530
FatI CATG 5 cut(s) 289, 343, 530, 618, 1023
FauI CCCGC 2 cut(s) 138, 669
FblI GTMKAC 3 cut(s) 90, 237, 420
Fnu4HI GCNGC 6 cut(s) 223, 259, 327, 350, 441, 962
FokI GGATG 3 cut(s) 95, 713, 797
Fsp4HI GCNGC 6 cut(s) 223, 259, 327, 350, 441, 962
FspBI CTAG 1 cut(s) 395
FspI TGCGCA 1 cut(s) 599
GlaI GCGC 4 cut(s) 250, 369, 452, 599
GluI GCNGC 6 cut(s) 223, 259, 327, 350, 441, 962
GsaI CCCAGC 1 cut(s) 572
HaeII RGCGCY 1 cut(s) 252
HaeIII GGCC 5 cut(s) 84, 393, 434, 477, 651
HapII CCGG 5 cut(s) 503, 713, 801, 874, 980
HgaI GACGC 1 cut(s) 182
HhaI GCGC 4 cut(s) 251, 370, 453, 600
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 5 cut(s) 293, 347, 534, 622, 1027
Hin6I GCGC 4 cut(s) 249, 368, 451, 598
HinP1I GCGC 4 cut(s) 249, 368, 451, 598
HincII GTYRAC 4 cut(s) 91, 238, 421, 723
HindII GTYRAC 4 cut(s) 91, 238, 421, 723
HindIII AAGCTT 1 cut(s) 68
HinfI GANTC 4 cut(s) 62, 75, 92, 886
HpaII CCGG 5 cut(s) 503, 713, 801, 874, 980
HphI GGTGA 5 cut(s) 274, 478, 652, 836, 863
Hpy166II GTNNAC 4 cut(s) 91, 238, 421, 723
Hpy188I TCNGA 5 cut(s) 205, 217, 630, 837, 1018
Hpy188III TCNNGA 5 cut(s) 277, 503, 737, 980, 1024
Hpy8I GTNNAC 4 cut(s) 91, 238, 421, 723
Hpy99I CGWCG 4 cut(s) 62, 176, 358, 422
HpyAV CCTTC 4 cut(s) 309, 578, 607, 630
HpyCH4III ACNGT 5 cut(s) 150, 317, 380, 552, 688
HpyCH4IV ACGT 2 cut(s) 417, 623
HpyCH4V TGCA 3 cut(s) 154, 530, 757
HpyF10VI GCNNNNNNNGC 4 cut(s) 255, 440, 457, 763
HpyF3I CTNAG 3 cut(s) 910, 1063, 1077
HpySE526I ACGT 2 cut(s) 417, 623
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 5 cut(s) 293, 347, 534, 622, 1027
HspAI GCGC 4 cut(s) 249, 368, 451, 598
Kpn2I TCCGGA 2 cut(s) 502, 979
Kzo9I GATC 7 cut(s) 217, 279, 321, 543, 679, 739, 868
Lsp1109I GCAGC 3 cut(s) 361, 427, 948
MaeI CTAG 1 cut(s) 395
MaeII ACGT 2 cut(s) 417, 623
MaeIII GTNAC 1 cut(s) 707
MalI GATC 7 cut(s) 219, 281, 323, 545, 681, 741, 870
MboI GATC 7 cut(s) 217, 279, 321, 543, 679, 739, 868
MboII GAAGA 3 cut(s) 53, 265, 331
MflI RGATCY 1 cut(s) 543
MhlI GDGCHC 1 cut(s) 808
MluCI AATT 6 cut(s) 53, 371, 522, 1019, 1046, 1069
MlyI GAGTC 3 cut(s) 56, 84, 86
MmeI TCCRAC 3 cut(s) 228, 438, 571
MroI TCCGGA 2 cut(s) 502, 979
MroXI GAANNNNTTC 1 cut(s) 53
MseI TTAA 4 cut(s) 363, 762, 816, 1008
MslI CAYNNNNRTG 1 cut(s) 747
MspA1I CMGCKG 2 cut(s) 447, 961
MspI CCGG 5 cut(s) 503, 713, 801, 874, 980
MspR9I CCNGG 5 cut(s) 86, 713, 714, 801, 875
MvaI CCWGG 1 cut(s) 86
MvnI CGCG 1 cut(s) 453
MwoI GCNNNNNNNGC 4 cut(s) 255, 440, 457, 763
NciI CCSGG 4 cut(s) 713, 714, 801, 875
NcoI CCATGG 1 cut(s) 289
NdeII GATC 7 cut(s) 217, 279, 321, 543, 679, 739, 868
NlaIII CATG 5 cut(s) 293, 347, 534, 622, 1027
NlaIV GGNNCC 2 cut(s) 392, 536
NmeAIII GCCGAG 1 cut(s) 453
NmuCI GTSAC 1 cut(s) 707
NsbI TGCGCA 1 cut(s) 599
PagI TCATGA 1 cut(s) 1023
PceI AGGCCT 1 cut(s) 84
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PdmI GAANNNNTTC 1 cut(s) 53
PfeI GAWTC 1 cut(s) 886
Pfl23II CGTACG 1 cut(s) 317
PflFI GACNNNGTC 1 cut(s) 242
PflMI CCANNNNNTGG 1 cut(s) 553
PkrI GCNGC 6 cut(s) 224, 260, 328, 351, 442, 963
Ple19I CGATCG 1 cut(s) 324
PleI GAGTC 3 cut(s) 56, 83, 86
PpsI GAGTC 3 cut(s) 56, 83, 86
PshAI GACNNNNGTC 1 cut(s) 96
PshBI ATTAAT 1 cut(s) 816
Psp6I CCWGG 1 cut(s) 84
PspFI CCCAGC 1 cut(s) 568
PspGI CCWGG 1 cut(s) 84
PspLI CGTACG 1 cut(s) 317
PspN4I GGNNCC 2 cut(s) 392, 536
PspPI GGNCC 5 cut(s) 313, 391, 476, 649, 956
PsuI RGATCY 1 cut(s) 543
PsyI GACNNNGTC 1 cut(s) 242
PvuI CGATCG 1 cut(s) 324
PvuII CAGCTG 1 cut(s) 447
RsaI GTAC 2 cut(s) 319, 428
RsaNI GTAC 2 cut(s) 318, 427
RseI CAYNNNNRTG 1 cut(s) 747
SalI GTCGAC 3 cut(s) 89, 236, 419
SaqAI TTAA 4 cut(s) 363, 762, 816, 1008
SatI GCNGC 6 cut(s) 223, 259, 327, 350, 441, 962
Sau3AI GATC 7 cut(s) 217, 279, 321, 543, 679, 739, 868
Sau96I GGNCC 5 cut(s) 313, 391, 476, 649, 956
SchI GAGTC 3 cut(s) 56, 84, 86
ScrFI CCNGG 5 cut(s) 86, 713, 714, 801, 875
SduI GDGCHC 1 cut(s) 808
SinI GGWCC 2 cut(s) 313, 956
SmaI CCCGGG 1 cut(s) 714
SmiMI CAYNNNNRTG 1 cut(s) 747
Sse9I AATT 6 cut(s) 53, 371, 522, 1019, 1046, 1069
SseBI AGGCCT 1 cut(s) 84
SsiI CCGC 7 cut(s) 131, 223, 259, 327, 488, 662, 959
SspI AATATT 1 cut(s) 13
SspMI CTAG 1 cut(s) 395
StuI AGGCCT 1 cut(s) 84
StyD4I CCNGG 5 cut(s) 84, 711, 712, 799, 873
StyI CCWWGG 2 cut(s) 289, 394
TaaI ACNGT 5 cut(s) 150, 317, 380, 552, 688
TaiI ACGT 2 cut(s) 420, 626
TaqI TCGA 5 cut(s) 57, 90, 195, 237, 420
TasI AATT 6 cut(s) 53, 371, 522, 1019, 1046, 1069
TatI WGTACW 1 cut(s) 426
TauI GCSGC 3 cut(s) 225, 261, 329
TfiI GAWTC 1 cut(s) 886
Tru1I TTAA 4 cut(s) 363, 762, 816, 1008
Tru9I TTAA 4 cut(s) 363, 762, 816, 1008
TseFI GTSAC 1 cut(s) 707
TseI GCWGC 3 cut(s) 349, 440, 961
Tsp45I GTSAC 1 cut(s) 707
TspDTI ATGAA 1 cut(s) 1012
TspGWI ACGGA 2 cut(s) 371, 617
TspMI CCCGGG 1 cut(s) 712
Tth111I GACNNNGTC 1 cut(s) 242
Van91I CCANNNNNTGG 1 cut(s) 553
VpaK11BI GGWCC 2 cut(s) 313, 956
VspI ATTAAT 1 cut(s) 816
XagI CCTNNNNNAGG 1 cut(s) 428
XapI RAATTY 1 cut(s) 1019
XmaI CCCGGG 1 cut(s) 712
XmaJI CCTAGG 1 cut(s) 394
XmiI GTMKAC 3 cut(s) 90, 237, 420
XmnI GAANNNNTTC 1 cut(s) 53
XspI CTAG 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.