MD16G1019800.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001116
Physical Location & Seq
Reverse (-)
1429430 .. 1431589
2160 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1019800.v1.1.491

Sequence Viewer

Length: 444 bp
ATGGTGGGGAATTACTACCCGTACTGCCCGCAACCTGATCTAACGGTTGGGCTTACATCTCATTCTGACCCAGGAGTGCTGACCGTGCTGCTTCAGGATCACATGGGTGGGCTGCAGGTGAAGTGCGGTGAGGGTTGGGTGGATGTGAAACCTGTCCCCGGTGCTCTCGTTATCAACGTTGGGGATTTTCTTCAGATTATTTCCAATGGTGAATACCAAAGTGTAGCTCATCGTGTGTTGGCGAACTCATCCCGTGAACCACGTGTGGCCATCGCTGTGTTTTTCAATTCGAGCAGAAGGGAGGACTCGTATGGGCCATTGCCGGAGCTAGTCTCCCCGTTGAAGCCGGCACTTTACCGGCAGTTTACGCTCTCGGAGTTCATGCAAAGGTTCTTCCAGAAAGGATTGGATGGCAAAGCTTTGCTAAATCACTTTGAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

148

Amino Acids

16.31

Weight (kDa)

6.04

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 1 - 96 4e-32 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 106
Acc36I ACCTGC 1 cut(s) 106
AciI CCGC 2 cut(s) 29, 126
AclI AACGTT 1 cut(s) 177
AclWI GGATC 1 cut(s) 105
AcoI YGGCCR 1 cut(s) 267
AcuI CTGAAG 2 cut(s) 77, 176
AcvI CACGTG 1 cut(s) 263
AfaI GTAC 1 cut(s) 23
AfiI CCNNNNNNNGG 1 cut(s) 158
AflIII ACRYGT 1 cut(s) 262
AgsI TTSAA 2 cut(s) 286, 343
AjnI CCWGG 1 cut(s) 70
AleI CACNNNNGTG 1 cut(s) 105
AluBI AGCT 4 cut(s) 227, 328, 419, 439
AluI AGCT 4 cut(s) 227, 328, 419, 439
Alw21I GWGCWC 1 cut(s) 166
Alw26I GTCTC 1 cut(s) 337
AlwI GGATC 1 cut(s) 105
AoxI GGCC 2 cut(s) 267, 314
ApeKI GCWGC 2 cut(s) 88, 112
AspS9I GGNCC 1 cut(s) 314
AsuC2I CCSGG 1 cut(s) 159
AsuHPI GGTGA 3 cut(s) 130, 140, 221
BalI TGGCCA 1 cut(s) 269
BbrPI CACGTG 1 cut(s) 263
Bbv12I GWGCWC 1 cut(s) 166
BbvI GCAGC 2 cut(s) 75, 99
BccI CCATC 2 cut(s) 278, 404
BciT130I CCWGG 1 cut(s) 72
BcnI CCSGG 1 cut(s) 159
BcoDI GTCTC 1 cut(s) 337
BfaI CTAG 1 cut(s) 329
BfmI CTRYAG 1 cut(s) 113
BfuAI ACCTGC 1 cut(s) 106
BisI GCNGC 2 cut(s) 89, 113
BlsI GCNGC 2 cut(s) 90, 114
Bme1390I CCNGG 2 cut(s) 72, 159
BmgT120I GGNCC 1 cut(s) 314
BmrFI CCNGG 2 cut(s) 72, 159
BplI GAGNNNNNCTC 2 cut(s) 317, 349
BpuMI CCSGG 1 cut(s) 159
BsaAI YACGTR 1 cut(s) 263
BsaJI CCNNGG 2 cut(s) 70, 157
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 1 cut(s) 158
Bse118I RCCGGY 2 cut(s) 346, 357
Bse3DI GCAATG 1 cut(s) 317
BseBI CCWGG 1 cut(s) 72
BseDI CCNNGG 2 cut(s) 70, 157
BseGI GGATG 3 cut(s) 148, 248, 415
BseLI CCNNNNNNNGG 1 cut(s) 158
BseMI GCAATG 1 cut(s) 317
BseXI GCAGC 2 cut(s) 75, 99
BshFI GGCC 2 cut(s) 269, 316
BsiHKAI GWGCWC 1 cut(s) 166
BsiSI CCGG 4 cut(s) 159, 323, 347, 358
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 158
BsmAI GTCTC 1 cut(s) 337
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 2 cut(s) 269, 316
Bsp1286I GDGCHC 1 cut(s) 166
Bsp143I GATC 2 cut(s) 37, 97
BspACI CCGC 2 cut(s) 29, 126
BspANI GGCC 2 cut(s) 269, 316
BspMAI CTGCAG 1 cut(s) 117
BspMI ACCTGC 1 cut(s) 106
BspPI GGATC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 317
BsrFI RCCGGY 2 cut(s) 346, 357
BssAI RCCGGY 2 cut(s) 346, 357
BssECI CCNNGG 2 cut(s) 70, 157
BssMI GATC 2 cut(s) 37, 97
Bst2UI CCWGG 1 cut(s) 72
Bst4CI ACNGT 2 cut(s) 46, 85
BstBAI YACGTR 1 cut(s) 263
BstC8I GCNNGC 2 cut(s) 29, 348
BstF5I GGATG 3 cut(s) 148, 248, 415
BstKTI GATC 2 cut(s) 40, 100
BstMAI GTCTC 1 cut(s) 337
BstMBI GATC 2 cut(s) 37, 97
BstMWI GCNNNNNNNGC 2 cut(s) 85, 367
BstNI CCWGG 1 cut(s) 72
BstSCI CCNGG 2 cut(s) 70, 157
BstSFI CTRYAG 1 cut(s) 113
BstV1I GCAGC 2 cut(s) 75, 99
BsuRI GGCC 2 cut(s) 269, 316
BtgZI GCGATG 1 cut(s) 256
BtsCI GGATG 3 cut(s) 148, 248, 415
BveI ACCTGC 1 cut(s) 106
Cac8I GCNNGC 2 cut(s) 29, 348
Cfr10I RCCGGY 2 cut(s) 346, 357
Cfr13I GGNCC 1 cut(s) 314
Csp6I GTAC 1 cut(s) 22
CviAII CATG 2 cut(s) 103, 382
CviJI RGCY 9 cut(s) 52, 112, 227, 269, 316, 328, 346, 419, 439
CviKI_1 RGCY 9 cut(s) 52, 112, 227, 269, 316, 328, 346, 419, 439
CviQI GTAC 1 cut(s) 22
DpnI GATC 2 cut(s) 39, 99
DpnII GATC 2 cut(s) 37, 97
EaeI YGGCCR 1 cut(s) 267
Eco57I CTGAAG 2 cut(s) 77, 176
Eco72I CACGTG 1 cut(s) 263
EcoRII CCWGG 1 cut(s) 70
FaeI CATG 2 cut(s) 106, 385
FaiI YATR 3 cut(s) 104, 312, 383
FaqI GGGAC 1 cut(s) 140
FatI CATG 2 cut(s) 102, 381
FauI CCCGC 1 cut(s) 36
Fnu4HI GCNGC 2 cut(s) 89, 113
FokI GGATG 3 cut(s) 155, 235, 422
Fsp4HI GCNGC 2 cut(s) 89, 113
FspBI CTAG 1 cut(s) 329
GluI GCNGC 2 cut(s) 89, 113
HaeIII GGCC 2 cut(s) 269, 316
HapII CCGG 4 cut(s) 159, 323, 347, 358
Hin1II CATG 2 cut(s) 106, 385
HindIII AAGCTT 1 cut(s) 417
HinfI GANTC 1 cut(s) 305
HpaII CCGG 4 cut(s) 159, 323, 347, 358
HphI GGTGA 3 cut(s) 130, 140, 221
Hpy166II GTNNAC 2 cut(s) 257, 366
Hpy188I TCNGA 3 cut(s) 67, 195, 376
Hpy188III TCNNGA 2 cut(s) 95, 397
Hpy8I GTNNAC 2 cut(s) 257, 366
HpyAV CCTTC 1 cut(s) 291
HpyCH4III ACNGT 2 cut(s) 46, 85
HpyCH4IV ACGT 2 cut(s) 177, 262
HpyCH4V TGCA 2 cut(s) 115, 385
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 367
HpySE526I ACGT 2 cut(s) 177, 262
Hsp92II CATG 2 cut(s) 106, 385
KroI GCCGGC 1 cut(s) 346
KroNI GCCGGC 1 cut(s) 348
Kzo9I GATC 2 cut(s) 37, 97
LmnI GCTCC 1 cut(s) 325
Lsp1109I GCAGC 2 cut(s) 75, 99
MaeI CTAG 1 cut(s) 329
MaeII ACGT 2 cut(s) 177, 262
MalI GATC 2 cut(s) 39, 99
MboI GATC 2 cut(s) 37, 97
MboII GAAGA 2 cut(s) 182, 385
MhlI GDGCHC 1 cut(s) 166
MlsI TGGCCA 1 cut(s) 269
MluCI AATT 2 cut(s) 10, 286
MluNI TGGCCA 1 cut(s) 269
MlyI GAGTC 1 cut(s) 299
MnlI CCTC 2 cut(s) 124, 295
Mox20I TGGCCA 1 cut(s) 269
MroNI GCCGGC 1 cut(s) 346
MscI TGGCCA 1 cut(s) 269
MslI CAYNNNNRTG 2 cut(s) 105, 275
Msp20I TGGCCA 1 cut(s) 269
MspI CCGG 4 cut(s) 159, 323, 347, 358
MspR9I CCNGG 2 cut(s) 72, 159
MvaI CCWGG 1 cut(s) 72
MwoI GCNNNNNNNGC 2 cut(s) 85, 367
NaeI GCCGGC 1 cut(s) 348
NciI CCSGG 1 cut(s) 159
NdeII GATC 2 cut(s) 37, 97
NgoMIV GCCGGC 1 cut(s) 346
NlaIII CATG 2 cut(s) 106, 385
OliI CACNNNNGTG 1 cut(s) 105
PaqCI CACCTGC 1 cut(s) 106
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PdiI GCCGGC 1 cut(s) 348
PkrI GCNGC 2 cut(s) 90, 114
PleI GAGTC 1 cut(s) 299
PmaCI CACGTG 1 cut(s) 263
PmlI CACGTG 1 cut(s) 263
PpsI GAGTC 1 cut(s) 299
Ppu21I YACGTR 1 cut(s) 263
Psp1406I AACGTT 1 cut(s) 177
Psp6I CCWGG 1 cut(s) 70
PspCI CACGTG 1 cut(s) 263
PspGI CCWGG 1 cut(s) 70
PspPI GGNCC 1 cut(s) 314
PstI CTGCAG 1 cut(s) 117
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
RseI CAYNNNNRTG 2 cut(s) 105, 275
SatI GCNGC 2 cut(s) 89, 113
Sau3AI GATC 2 cut(s) 37, 97
Sau96I GGNCC 1 cut(s) 314
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 2 cut(s) 72, 159
SduI GDGCHC 1 cut(s) 166
SfcI CTRYAG 1 cut(s) 113
SmiMI CAYNNNNRTG 2 cut(s) 105, 275
Sse9I AATT 2 cut(s) 10, 286
SsiI CCGC 2 cut(s) 29, 126
SspMI CTAG 1 cut(s) 329
StyD4I CCNGG 2 cut(s) 70, 157
TaaI ACNGT 2 cut(s) 46, 85
TaiI ACGT 2 cut(s) 180, 265
TaqI TCGA 1 cut(s) 290
TasI AATT 2 cut(s) 10, 286
TseI GCWGC 2 cut(s) 88, 112
TspDTI ATGAA 1 cut(s) 370
XspI CTAG 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.