Rroxscaffold_5G00383820

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001116
Physical Location & Seq
Reverse (-)
63389940 .. 63391282
1343 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00383820.1

Sequence Viewer

Length: 1065 bp
ATGGCGGCAAATGGTACCTTGATCGGCGAACAGGGTTATGATCGAGCCGAAGAAGTGAAGCAATTCGACGACTCCAAGCTCGGAGTCAAAGGCCTCGTCGACTCGGGTCTCTCCTCCATCCCCCGAATCTTCATCCACCCACCCGAAACCTTACAGCAATTGCAGCCCACGACCCGCCCCCACGCCCAATCAATCCCCACCATAGACCTCTCCGACCTCCACCACTCCGATCGCCGCTCCGCCATCGTTGACCAAGTCGGCGGCGCTTGCCGCAACTTCGGTTTCTTCCAGATCACCAACCACGGCATACCGACGGTGGTTCTGGACCGTACGATCGCCGCCGTCAAGGCCTTCCACGAGCAGCCGACGGAGGTCAAGGCGCGGTTCTACCGTAGAGAGATGGGCCAGGGCGTATCGTATCTCTCCAACGTTGACCTGTACACCTCCAAAGCCGCCAGCTGGCGCGACACCCTTCAGGTGAGGCTCGGCCCTGTTCCGGCGGCCGACGAGGAAGTTCCGGAGATTTGTAGGGATGAAATTGTGGCGTGGAACAAGGAGATCCGACGGCTGGGAGAGGAGCTGCTGGGGCTGGTGAGTGAAGGGCTGGGATTGCCCGCGGAGAAGCTGAAGGAGATGACGTGTCTGGGTGGGAGGGTGATGGCGGCCCACTACTACCCGCCGTGCCCGCAGCCTGATCTAACGGTTGGGCTTACGTCACACAGTGACCCTGGAGTGTTGACCTTAGTGCTTCAAGATCATATTGGTGGGTTGCAGGTTAAGCACGATGAGGCTTGGGTGGATGTGAAACCTATCCCCGGTGCACTCGTGATCAACGTTGGTGATTTACTCCAGATTATTTCAAACGAGGAATACAAAAGCGTGGATCACCGGGTGTTGGCAAATCCAAGCCATGAGCCACGTGTCTCGATCGCCGTGTTTTTGAACCCGAGCAATAGAGAGGGCGTGTTTGGTCCGTTGCCGGAGCTGGTCTCGCCGGAGAAGCCAGCTCTCTACAAAGAGTTCAGGTTTTCGGAATTCATGACGAGCCACTATCAGAAACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

354

Amino Acids

39.18

Weight (kDa)

5.64

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 65 - 162 2.2e-20 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 219 - 316 4.2e-33 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 763
Acc65I GGTACC 1 cut(s) 14
AccB1I GGYRCC 1 cut(s) 14
AccBSI CCGCTC 1 cut(s) 237
AccI GTMKAC 1 cut(s) 99
AccII CGCG 3 cut(s) 382, 465, 617
AccIII TCCGGA 1 cut(s) 517
AclI AACGTT 2 cut(s) 429, 834
AclWI GGATC 2 cut(s) 553, 891
AcoI YGGCCR 1 cut(s) 501
AcsI RAATTY 1 cut(s) 1034
AcuI CTGAAG 2 cut(s) 458, 647
AcvI CACGTG 1 cut(s) 920
AdeI CACNNNGTG 2 cut(s) 722, 892
AfaI GTAC 3 cut(s) 16, 331, 440
AfiI CCNNNNNNNGG 5 cut(s) 459, 496, 568, 815, 895
AflIII ACRYGT 2 cut(s) 638, 919
AgsI TTSAA 3 cut(s) 752, 861, 943
AjiI CACGTC 1 cut(s) 639
AjnI CCWGG 2 cut(s) 405, 727
AluBI AGCT 6 cut(s) 79, 459, 580, 625, 985, 1007
AluI AGCT 6 cut(s) 79, 459, 580, 625, 985, 1007
Alw21I GWGCWC 1 cut(s) 823
Alw26I GTCTC 3 cut(s) 113, 928, 994
Alw44I GTGCAC 1 cut(s) 819
AlwI GGATC 2 cut(s) 553, 891
Ama87I CYCGRG 2 cut(s) 103, 946
Aor13HI TCCGGA 1 cut(s) 517
AoxI GGCC 6 cut(s) 91, 348, 403, 487, 501, 663
ApaLI GTGCAC 1 cut(s) 819
ApeKI GCWGC 4 cut(s) 163, 361, 580, 688
ApoI RAATTY 1 cut(s) 1034
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
Asp700I GAANNNNTTC 1 cut(s) 62
Asp718I GGTACC 1 cut(s) 14
AspLEI GCGC 3 cut(s) 266, 382, 465
AspS9I GGNCC 5 cut(s) 325, 403, 488, 664, 971
AsuC2I CCSGG 2 cut(s) 816, 890
AsuHPI GGTGA 6 cut(s) 286, 490, 604, 667, 851, 878
AvaI CYCGRG 2 cut(s) 103, 946
AvaII GGWCC 2 cut(s) 325, 971
BaeGI GKGCMC 2 cut(s) 686, 823
BanI GGYRCC 1 cut(s) 14
BauI CACGAG 2 cut(s) 356, 824
BbrPI CACGTG 1 cut(s) 920
Bbv12I GWGCWC 1 cut(s) 823
BbvI GCAGC 4 cut(s) 175, 373, 567, 700
BccI CCATC 4 cut(s) 125, 251, 394, 652
BceAI ACGGC 5 cut(s) 319, 326, 581, 664, 917
BciT130I CCWGG 2 cut(s) 407, 729
BclI TGATCA 1 cut(s) 828
BcnI CCSGG 2 cut(s) 816, 890
BcoDI GTCTC 3 cut(s) 113, 928, 994
BfoI RGCGCY 1 cut(s) 267
BfuAI ACCTGC 1 cut(s) 763
BglI GCCNNNNNGGC 1 cut(s) 347
Bme1390I CCNGG 4 cut(s) 407, 729, 816, 890
Bme18I GGWCC 2 cut(s) 325, 971
BmeT110I CYCGRG 2 cut(s) 103, 946
BmgBI CACGTC 1 cut(s) 639
BmgT120I GGNCC 5 cut(s) 325, 403, 488, 664, 971
BmiI GGNNCC 1 cut(s) 16
BmrFI CCNGG 4 cut(s) 407, 729, 816, 890
BoxI GACNNNNGTC 2 cut(s) 105, 371
BplI GAGNNNNNCTC 2 cut(s) 974, 1006
BpmI CTGGAG 2 cut(s) 750, 833
BpuMI CCSGG 2 cut(s) 816, 890
BsaAI YACGTR 1 cut(s) 920
BsaI GGTCTC 2 cut(s) 113, 994
BsaJI CCNNGG 5 cut(s) 301, 406, 615, 727, 814
BsaWI WCCGGW 1 cut(s) 517
BsaXI ACNNNNNCTCC 2 cut(s) 623, 653
Bsc4I CCNNNNNNNGG 5 cut(s) 459, 496, 568, 815, 895
BseAI TCCGGA 1 cut(s) 517
BseBI CCWGG 2 cut(s) 407, 729
BseDI CCNNGG 5 cut(s) 301, 406, 615, 727, 814
BseGI GGATG 4 cut(s) 117, 132, 538, 805
BseLI CCNNNNNNNGG 5 cut(s) 459, 496, 568, 815, 895
BseRI GAGGAG 2 cut(s) 103, 590
BseSI GKGCMC 2 cut(s) 686, 823
BseX3I CGGCCG 1 cut(s) 501
BseXI GCAGC 4 cut(s) 175, 373, 567, 700
BseYI CCCAGC 3 cut(s) 568, 583, 604
Bsh1236I CGCG 3 cut(s) 382, 465, 617
Bsh1285I CGRYCG 4 cut(s) 232, 336, 504, 930
BshFI GGCC 6 cut(s) 93, 350, 405, 489, 503, 665
BshNI GGYRCC 1 cut(s) 14
BsiEI CGRYCG 4 cut(s) 232, 336, 504, 930
BsiHKAI GWGCWC 1 cut(s) 823
BsiHKCI CYCGRG 2 cut(s) 103, 946
BsiSI CCGG 6 cut(s) 497, 518, 816, 889, 980, 995
BsiWI CGTACG 1 cut(s) 329
BslI CCNNNNNNNGG 5 cut(s) 459, 496, 568, 815, 895
BsmAI GTCTC 3 cut(s) 113, 928, 994
BsnI GGCC 6 cut(s) 93, 350, 405, 489, 503, 665
Bso31I GGTCTC 2 cut(s) 113, 994
BsoBI CYCGRG 2 cut(s) 103, 946
Bsp1286I GDGCHC 2 cut(s) 686, 823
Bsp13I TCCGGA 1 cut(s) 517
Bsp1407I TGTACA 1 cut(s) 438
BspANI GGCC 6 cut(s) 93, 350, 405, 489, 503, 665
BspEI TCCGGA 1 cut(s) 517
BspFNI CGCG 3 cut(s) 382, 465, 617
BspHI TCATGA 1 cut(s) 1038
BspLI GGNNCC 1 cut(s) 16
BspMI ACCTGC 1 cut(s) 763
BspPI GGATC 2 cut(s) 553, 891
BspT107I GGYRCC 1 cut(s) 14
BspTNI GGTCTC 2 cut(s) 113, 994
BsrBI CCGCTC 1 cut(s) 237
BsrGI TGTACA 1 cut(s) 438
BssECI CCNNGG 5 cut(s) 301, 406, 615, 727, 814
BssSI CACGAG 2 cut(s) 356, 824
Bst2BI CACGAG 2 cut(s) 356, 824
Bst2UI CCWGG 2 cut(s) 407, 729
Bst4CI ACNGT 5 cut(s) 316, 329, 392, 703, 722
BstAUI TGTACA 1 cut(s) 438
BstBAI YACGTR 1 cut(s) 920
BstC8I GCNNGC 6 cut(s) 268, 457, 461, 615, 686, 1005
BstDEI CTNAG 1 cut(s) 742
BstDSI CCRYGG 2 cut(s) 301, 615
BstF5I GGATG 4 cut(s) 117, 132, 538, 805
BstFNI CGCG 3 cut(s) 382, 465, 617
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 3 cut(s) 266, 382, 465
BstMAI GTCTC 3 cut(s) 113, 928, 994
BstMCI CGRYCG 4 cut(s) 232, 336, 504, 930
BstNI CCWGG 2 cut(s) 407, 729
BstPAI GACNNNNGTC 2 cut(s) 105, 371
BstSCI CCNGG 4 cut(s) 405, 727, 814, 888
BstSLI GKGCMC 2 cut(s) 686, 823
BstUI CGCG 3 cut(s) 382, 465, 617
BstV1I GCAGC 4 cut(s) 175, 373, 567, 700
BstX2I RGATCY 1 cut(s) 558
BstYI RGATCY 1 cut(s) 558
BstZI CGGCCG 1 cut(s) 501
BsuRI GGCC 6 cut(s) 93, 350, 405, 489, 503, 665
BtgI CCRYGG 2 cut(s) 301, 615
BtrI CACGTC 1 cut(s) 639
BtsCI GGATG 4 cut(s) 117, 132, 538, 805
BtsIMutI CAGTG 1 cut(s) 727
BveI ACCTGC 1 cut(s) 763
Cac8I GCNNGC 6 cut(s) 268, 457, 461, 615, 686, 1005
CciI TCATGA 1 cut(s) 1038
CfoI GCGC 3 cut(s) 266, 382, 465
Cfr13I GGNCC 5 cut(s) 325, 403, 488, 664, 971
Cfr42I CCGCGG 1 cut(s) 618
Csp6I GTAC 3 cut(s) 15, 330, 439
CviAII CATG 2 cut(s) 911, 1039
CviQI GTAC 3 cut(s) 15, 330, 439
DdeI CTNAG 1 cut(s) 742
DraIII CACNNNGTG 2 cut(s) 722, 892
EaeI YGGCCR 1 cut(s) 501
EagI CGGCCG 1 cut(s) 501
EciI GGCGGA 1 cut(s) 229
EclXI CGGCCG 1 cut(s) 501
Eco147I AGGCCT 2 cut(s) 93, 350
Eco31I GGTCTC 2 cut(s) 113, 994
Eco47I GGWCC 2 cut(s) 325, 971
Eco52I CGGCCG 1 cut(s) 501
Eco57I CTGAAG 2 cut(s) 458, 647
Eco72I CACGTG 1 cut(s) 920
Eco88I CYCGRG 2 cut(s) 103, 946
EcoRI GAATTC 1 cut(s) 1034
EcoRII CCWGG 2 cut(s) 405, 727
FaeI CATG 2 cut(s) 914, 1042
FaiI YATR 7 cut(s) 39, 203, 308, 759, 912, 1040, 1063
FatI CATG 2 cut(s) 910, 1038
FauI CCCGC 4 cut(s) 182, 622, 684, 693
FbaI TGATCA 1 cut(s) 828
FblI GTMKAC 1 cut(s) 99
FokI GGATG 4 cut(s) 104, 119, 545, 812
GlaI GCGC 3 cut(s) 265, 381, 464
GsaI CCCAGC 3 cut(s) 572, 587, 608
GsuI CTGGAG 2 cut(s) 750, 833
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 6 cut(s) 93, 350, 405, 489, 503, 665
HapII CCGG 6 cut(s) 497, 518, 816, 889, 980, 995
HhaI GCGC 3 cut(s) 266, 382, 465
Hin1II CATG 2 cut(s) 914, 1042
Hin6I GCGC 3 cut(s) 264, 380, 463
HinP1I GCGC 3 cut(s) 264, 380, 463
HincII GTYRAC 4 cut(s) 100, 250, 433, 738
HindII GTYRAC 4 cut(s) 100, 250, 433, 738
HinfI GANTC 4 cut(s) 71, 84, 101, 126
HpaII CCGG 6 cut(s) 497, 518, 816, 889, 980, 995
HphI GGTGA 6 cut(s) 286, 490, 604, 667, 851, 878
Hpy166II GTNNAC 6 cut(s) 100, 250, 433, 441, 738, 821
Hpy188I TCNGA 6 cut(s) 83, 214, 229, 563, 1033, 1056
Hpy188III TCNNGA 8 cut(s) 289, 323, 518, 752, 826, 850, 925, 1039
Hpy8I GTNNAC 6 cut(s) 100, 250, 433, 441, 738, 821
Hpy99I CGWCG 6 cut(s) 71, 101, 316, 370, 509, 567
HpyAV CCTTC 4 cut(s) 361, 482, 593, 622
HpyCH4III ACNGT 5 cut(s) 316, 329, 392, 703, 722
HpyCH4IV ACGT 5 cut(s) 429, 638, 713, 834, 919
HpyCH4V TGCA 3 cut(s) 163, 772, 821
HpyF3I CTNAG 1 cut(s) 742
HpySE526I ACGT 5 cut(s) 429, 638, 713, 834, 919
Hsp92II CATG 2 cut(s) 914, 1042
HspAI GCGC 3 cut(s) 264, 380, 463
Kpn2I TCCGGA 1 cut(s) 517
KpnI GGTACC 1 cut(s) 18
Ksp22I TGATCA 1 cut(s) 828
KspI CCGCGG 1 cut(s) 618
LmnI GCTCC 3 cut(s) 242, 577, 982
Lsp1109I GCAGC 4 cut(s) 175, 373, 567, 700
MaeII ACGT 5 cut(s) 429, 638, 713, 834, 919
MaeIII GTNAC 2 cut(s) 714, 722
MbiI CCGCTC 1 cut(s) 237
MboII GAAGA 3 cut(s) 62, 121, 277
MfeI CAATTG 1 cut(s) 158
MflI RGATCY 1 cut(s) 558
MhlI GDGCHC 2 cut(s) 686, 823
MluCI AATT 4 cut(s) 62, 158, 537, 1034
MlyI GAGTC 3 cut(s) 65, 93, 95
MmeI TCCRAC 3 cut(s) 237, 450, 586
MroI TCCGGA 1 cut(s) 517
MroXI GAANNNNTTC 1 cut(s) 62
MseI TTAA 1 cut(s) 777
MslI CAYNNNNRTG 1 cut(s) 762
MspA1I CMGCKG 2 cut(s) 459, 617
MspI CCGG 6 cut(s) 497, 518, 816, 889, 980, 995
MspR9I CCNGG 4 cut(s) 407, 729, 816, 890
MunI CAATTG 1 cut(s) 158
MvaI CCWGG 2 cut(s) 407, 729
MvnI CGCG 3 cut(s) 382, 465, 617
NciI CCSGG 2 cut(s) 816, 890
NlaIII CATG 2 cut(s) 914, 1042
NlaIV GGNNCC 1 cut(s) 16
NmeAIII GCCGAG 1 cut(s) 465
NmuCI GTSAC 2 cut(s) 714, 722
PagI TCATGA 1 cut(s) 1038
PceI AGGCCT 2 cut(s) 93, 350
PcsI WCGNNNNNNNCGW 1 cut(s) 831
PdmI GAANNNNTTC 1 cut(s) 62
PfeI GAWTC 1 cut(s) 126
Pfl23II CGTACG 1 cut(s) 329
PflFI GACNNNGTC 1 cut(s) 254
Ple19I CGATCG 3 cut(s) 232, 336, 930
PleI GAGTC 3 cut(s) 65, 92, 95
PmaCI CACGTG 1 cut(s) 920
PmlI CACGTG 1 cut(s) 920
PpsI GAGTC 3 cut(s) 65, 92, 95
Ppu21I YACGTR 1 cut(s) 920
PshAI GACNNNNGTC 2 cut(s) 105, 371
Psp1406I AACGTT 2 cut(s) 429, 834
Psp6I CCWGG 2 cut(s) 405, 727
PspCI CACGTG 1 cut(s) 920
PspFI CCCAGC 3 cut(s) 568, 583, 604
PspGI CCWGG 2 cut(s) 405, 727
PspLI CGTACG 1 cut(s) 329
PspN4I GGNNCC 1 cut(s) 16
PspPI GGNCC 5 cut(s) 325, 403, 488, 664, 971
PsuI RGATCY 1 cut(s) 558
PsyI GACNNNGTC 1 cut(s) 254
PvuI CGATCG 3 cut(s) 232, 336, 930
PvuII CAGCTG 1 cut(s) 459
RsaI GTAC 3 cut(s) 16, 331, 440
RsaNI GTAC 3 cut(s) 15, 330, 439
RseI CAYNNNNRTG 1 cut(s) 762
SacII CCGCGG 1 cut(s) 618
SalI GTCGAC 1 cut(s) 98
SaqAI TTAA 1 cut(s) 777
Sau96I GGNCC 5 cut(s) 325, 403, 488, 664, 971
SchI GAGTC 3 cut(s) 65, 93, 95
ScrFI CCNGG 4 cut(s) 407, 729, 816, 890
SduI GDGCHC 2 cut(s) 686, 823
Sfr303I CCGCGG 1 cut(s) 618
SgrBI CCGCGG 1 cut(s) 618
SinI GGWCC 2 cut(s) 325, 971
SmiMI CAYNNNNRTG 1 cut(s) 762
Sse9I AATT 4 cut(s) 62, 158, 537, 1034
SseBI AGGCCT 2 cut(s) 93, 350
StuI AGGCCT 2 cut(s) 93, 350
StyD4I CCNGG 4 cut(s) 405, 727, 814, 888
TaaI ACNGT 5 cut(s) 316, 329, 392, 703, 722
TaiI ACGT 5 cut(s) 432, 641, 716, 837, 922
TaqI TCGA 4 cut(s) 43, 66, 99, 926
TasI AATT 4 cut(s) 62, 158, 537, 1034
TatI WGTACW 1 cut(s) 438
TauI GCSGC 8 cut(s) 8, 237, 264, 273, 341, 455, 503, 665
TfiI GAWTC 1 cut(s) 126
Tru1I TTAA 1 cut(s) 777
Tru9I TTAA 1 cut(s) 777
TscAI CASTG 1 cut(s) 727
TseFI GTSAC 2 cut(s) 714, 722
TseI GCWGC 4 cut(s) 163, 361, 580, 688
Tsp45I GTSAC 2 cut(s) 714, 722
TspDTI ATGAA 3 cut(s) 121, 549, 1027
TspGWI ACGGA 2 cut(s) 383, 963
TspRI CASTG 1 cut(s) 727
Tth111I GACNNNGTC 1 cut(s) 254
VneI GTGCAC 1 cut(s) 819
VpaK11BI GGWCC 2 cut(s) 325, 971
XapI RAATTY 1 cut(s) 1034
XmiI GTMKAC 1 cut(s) 99
XmnI GAANNNNTTC 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.