RchiOBHm_Chr1g0333401

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001116
Physical Location & Seq
Reverse (-)
25524450 .. 25524878
429 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56220

Sequence Viewer

Length: 429 bp
ATGACCCGACCTGACGCCCGACCAATCCCCACCATCGACCTCTCCGACCTCCGCTCTACCATCATCGACCAAGTTAGCGGCACGTGCTGCAACTTCGGTTTCTTCCAGATCACCAACCACAGCATACCGTTGGTGGTTCTCGATAGTACGATCGCTGCTGTTAAGGCCTCCCACGAGCAGCCGACGACAGTCAAGGCGCGGTTCTACAGTAGAGAGATGGGCCAGGGCGTATCGTATCTCTCCAACGTCGACCTGTACACCTCCAGAGACGCCAACTGGCACGACACGCTTCAGGTAAGGCTCGGCCCTACTCCGGCGGCCGTTGACGAAGTTCCGGAGATTTGTAGAGATGAAATTGTGGCGTGGAACAAGGAGATCCAACCCACATTATTGGAGGATAATAATATGATTATTGGGGTAATAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

142

Amino Acids

15.8

Weight (kDa)

4.76

Isoelectric Point (pI)

32.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 9 - 100 6.4e-12 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 54
AccI GTMKAC 1 cut(s) 249
AccII CGCG 1 cut(s) 199
AccIII TCCGGA 1 cut(s) 334
AciI CCGC 4 cut(s) 52, 78, 199, 317
AclWI GGATC 1 cut(s) 370
AcoI YGGCCR 1 cut(s) 318
AcuI CTGAAG 1 cut(s) 275
AcvI CACGTG 1 cut(s) 84
AcyI GRCGYC 2 cut(s) 15, 270
AfaI GTAC 2 cut(s) 148, 257
AfiI CCNNNNNNNGG 1 cut(s) 313
AjnI CCWGG 1 cut(s) 222
Alw26I GTCTC 1 cut(s) 261
AlwI GGATC 1 cut(s) 370
Aor13HI TCCGGA 1 cut(s) 334
AoxI GGCC 4 cut(s) 165, 220, 304, 318
ApeKI GCWGC 3 cut(s) 87, 155, 178
AspLEI GCGC 1 cut(s) 199
AspS9I GGNCC 2 cut(s) 220, 305
AsuHPI GGTGA 1 cut(s) 103
BauI CACGAG 1 cut(s) 173
BbrPI CACGTG 1 cut(s) 84
BbvI GCAGC 3 cut(s) 74, 142, 190
BccI CCATC 3 cut(s) 41, 68, 211
BceAI ACGGC 1 cut(s) 305
BciT130I CCWGG 1 cut(s) 224
BcoDI GTCTC 1 cut(s) 261
BfmI CTRYAG 1 cut(s) 205
BisI GCNGC 5 cut(s) 79, 88, 156, 179, 318
BlsI GCNGC 5 cut(s) 80, 89, 157, 180, 319
Bme1390I CCNGG 1 cut(s) 224
BmgT120I GGNCC 2 cut(s) 220, 305
BmrFI CCNGG 1 cut(s) 224
BoxI GACNNNNGTC 1 cut(s) 188
BpmI CTGGAG 1 cut(s) 247
BsaAI YACGTR 1 cut(s) 84
BsaHI GRCGYC 2 cut(s) 15, 270
BsaJI CCNNGG 1 cut(s) 223
BsaWI WCCGGW 1 cut(s) 334
Bsc4I CCNNNNNNNGG 1 cut(s) 313
Bse1I ACTGG 1 cut(s) 281
BseAI TCCGGA 1 cut(s) 334
BseBI CCWGG 1 cut(s) 224
BseDI CCNNGG 1 cut(s) 223
BseLI CCNNNNNNNGG 1 cut(s) 313
BseNI ACTGG 1 cut(s) 281
BseX3I CGGCCG 1 cut(s) 318
BseXI GCAGC 3 cut(s) 74, 142, 190
Bsh1236I CGCG 1 cut(s) 199
Bsh1285I CGRYCG 2 cut(s) 153, 321
BshFI GGCC 4 cut(s) 167, 222, 306, 320
BsiEI CGRYCG 2 cut(s) 153, 321
BsiSI CCGG 2 cut(s) 314, 335
BslI CCNNNNNNNGG 1 cut(s) 313
BsmAI GTCTC 1 cut(s) 261
BsmBI CGTCTC 1 cut(s) 261
BsnI GGCC 4 cut(s) 167, 222, 306, 320
Bsp13I TCCGGA 1 cut(s) 334
Bsp1407I TGTACA 1 cut(s) 255
Bsp143I GATC 3 cut(s) 108, 150, 375
BspACI CCGC 4 cut(s) 52, 78, 199, 317
BspANI GGCC 4 cut(s) 167, 222, 306, 320
BspEI TCCGGA 1 cut(s) 334
BspFNI CGCG 1 cut(s) 199
BspPI GGATC 1 cut(s) 370
BsrBI CCGCTC 1 cut(s) 54
BsrGI TGTACA 1 cut(s) 255
BsrI ACTGG 1 cut(s) 281
BssECI CCNNGG 1 cut(s) 223
BssMI GATC 3 cut(s) 108, 150, 375
BssNI GRCGYC 2 cut(s) 15, 270
BssSI CACGAG 1 cut(s) 173
Bst2BI CACGAG 1 cut(s) 173
Bst2UI CCWGG 1 cut(s) 224
Bst4CI ACNGT 3 cut(s) 129, 190, 209
BstACI GRCGYC 2 cut(s) 15, 270
BstAPI GCANNNNNTGC 1 cut(s) 87
BstAUI TGTACA 1 cut(s) 255
BstBAI YACGTR 1 cut(s) 84
BstFNI CGCG 1 cut(s) 199
BstHHI GCGC 1 cut(s) 199
BstKTI GATC 3 cut(s) 111, 153, 378
BstMAI GTCTC 1 cut(s) 261
BstMBI GATC 3 cut(s) 108, 150, 375
BstMCI CGRYCG 2 cut(s) 153, 321
BstMWI GCNNNNNNNGC 4 cut(s) 84, 87, 164, 286
BstNI CCWGG 1 cut(s) 224
BstPAI GACNNNNGTC 1 cut(s) 188
BstSCI CCNGG 1 cut(s) 222
BstSFI CTRYAG 1 cut(s) 205
BstUI CGCG 1 cut(s) 199
BstV1I GCAGC 3 cut(s) 74, 142, 190
BstX2I RGATCY 1 cut(s) 375
BstXI CCANNNNNNTGG 1 cut(s) 391
BstYI RGATCY 1 cut(s) 375
BstZI CGGCCG 1 cut(s) 318
BsuRI GGCC 4 cut(s) 167, 222, 306, 320
CfoI GCGC 1 cut(s) 199
Cfr13I GGNCC 2 cut(s) 220, 305
CseI GACGC 2 cut(s) 23, 278
Csp6I GTAC 2 cut(s) 147, 256
CviJI RGCY 6 cut(s) 167, 181, 222, 301, 306, 320
CviKI_1 RGCY 6 cut(s) 167, 181, 222, 301, 306, 320
CviQI GTAC 2 cut(s) 147, 256
DpnI GATC 3 cut(s) 110, 152, 377
DpnII GATC 3 cut(s) 108, 150, 375
EaeI YGGCCR 1 cut(s) 318
EagI CGGCCG 1 cut(s) 318
EclXI CGGCCG 1 cut(s) 318
Eco147I AGGCCT 1 cut(s) 167
Eco52I CGGCCG 1 cut(s) 318
Eco57I CTGAAG 1 cut(s) 275
Eco72I CACGTG 1 cut(s) 84
EcoRII CCWGG 1 cut(s) 222
Esp3I CGTCTC 1 cut(s) 261
FaiI YATR 3 cut(s) 125, 407, 427
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 5 cut(s) 79, 88, 156, 179, 318
Fsp4HI GCNGC 5 cut(s) 79, 88, 156, 179, 318
GlaI GCGC 1 cut(s) 198
GluI GCNGC 5 cut(s) 79, 88, 156, 179, 318
GsuI CTGGAG 1 cut(s) 247
HaeIII GGCC 4 cut(s) 167, 222, 306, 320
HapII CCGG 2 cut(s) 314, 335
HgaI GACGC 2 cut(s) 23, 278
HhaI GCGC 1 cut(s) 199
Hin1I GRCGYC 2 cut(s) 15, 270
Hin6I GCGC 1 cut(s) 197
HinP1I GCGC 1 cut(s) 197
HincII GTYRAC 2 cut(s) 250, 325
HindII GTYRAC 2 cut(s) 250, 325
HpaII CCGG 2 cut(s) 314, 335
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 3 cut(s) 250, 258, 325
Hpy188I TCNGA 1 cut(s) 46
Hpy188III TCNNGA 4 cut(s) 106, 140, 264, 335
Hpy8I GTNNAC 3 cut(s) 250, 258, 325
Hpy99I CGWCG 2 cut(s) 187, 251
HpyCH4III ACNGT 3 cut(s) 129, 190, 209
HpyCH4IV ACGT 2 cut(s) 83, 246
HpyCH4V TGCA 1 cut(s) 90
HpyF10VI GCNNNNNNNGC 4 cut(s) 84, 87, 164, 286
HpySE526I ACGT 2 cut(s) 83, 246
Hsp92I GRCGYC 2 cut(s) 15, 270
HspAI GCGC 1 cut(s) 197
Kpn2I TCCGGA 1 cut(s) 334
Kzo9I GATC 3 cut(s) 108, 150, 375
Lsp1109I GCAGC 3 cut(s) 74, 142, 190
MaeII ACGT 2 cut(s) 83, 246
MalI GATC 3 cut(s) 110, 152, 377
MbiI CCGCTC 1 cut(s) 54
MboI GATC 3 cut(s) 108, 150, 375
MboII GAAGA 1 cut(s) 94
MflI RGATCY 1 cut(s) 375
MluCI AATT 1 cut(s) 354
MmeI TCCRAC 3 cut(s) 69, 267, 403
MnlI CCTC 5 cut(s) 50, 59, 178, 271, 388
MroI TCCGGA 1 cut(s) 334
MseI TTAA 1 cut(s) 162
MspI CCGG 2 cut(s) 314, 335
MspR9I CCNGG 1 cut(s) 224
MvaI CCWGG 1 cut(s) 224
MvnI CGCG 1 cut(s) 199
MwoI GCNNNNNNNGC 4 cut(s) 84, 87, 164, 286
NdeII GATC 3 cut(s) 108, 150, 375
NmeAIII GCCGAG 1 cut(s) 282
PceI AGGCCT 1 cut(s) 167
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PkrI GCNGC 5 cut(s) 80, 89, 157, 180, 319
Ple19I CGATCG 1 cut(s) 153
PmaCI CACGTG 1 cut(s) 84
PmlI CACGTG 1 cut(s) 84
Ppu21I YACGTR 1 cut(s) 84
PshAI GACNNNNGTC 1 cut(s) 188
Psp6I CCWGG 1 cut(s) 222
PspCI CACGTG 1 cut(s) 84
PspGI CCWGG 1 cut(s) 222
PspPI GGNCC 2 cut(s) 220, 305
PsuI RGATCY 1 cut(s) 375
PvuI CGATCG 1 cut(s) 153
RsaI GTAC 2 cut(s) 148, 257
RsaNI GTAC 2 cut(s) 147, 256
SalI GTCGAC 1 cut(s) 248
SaqAI TTAA 1 cut(s) 162
SatI GCNGC 5 cut(s) 79, 88, 156, 179, 318
Sau3AI GATC 3 cut(s) 108, 150, 375
Sau96I GGNCC 2 cut(s) 220, 305
ScrFI CCNGG 1 cut(s) 224
SetI ASST 8 cut(s) 13, 42, 51, 86, 249, 255, 263, 297
SfcI CTRYAG 1 cut(s) 205
Sse9I AATT 1 cut(s) 354
SseBI AGGCCT 1 cut(s) 167
SsiI CCGC 4 cut(s) 52, 78, 199, 317
StuI AGGCCT 1 cut(s) 167
StyD4I CCNGG 1 cut(s) 222
TaaI ACNGT 3 cut(s) 129, 190, 209
TaiI ACGT 2 cut(s) 86, 249
TaqI TCGA 4 cut(s) 36, 66, 141, 249
TasI AATT 1 cut(s) 354
TatI WGTACW 1 cut(s) 255
TauI GCSGC 2 cut(s) 81, 320
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TseI GCWGC 3 cut(s) 87, 155, 178
TspDTI ATGAA 1 cut(s) 366
XmiI GTMKAC 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.