Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0001335

Physical Location & Seq

Genomic Coordinates Reverse (-)

5561293 .. 5564187

Gene Structure 2895 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g09630.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1059 bp

ATGGACGTCCCAGAAGCTATGCAGCCAACTATTATTGATCAAATATTTCTGGGGGTCGAGGCGGCCGACGTCCCTAGGCGGCCTATGATTTGTGTCCTGATGGATAATACTCTCAGGATGATAAAACCCAAGATCCTCGTTGGTCTAGAATGGCTAGAGAAAGTGAGGCTTGATAGTTTCGAAGATACTGCTAGAGAAGAAGCATGTGTTTTTTGTACAGAGCCCTTTGATCACAATGCGTGTGTAGACGAAGGCATTCATGAGCAGCAACTGATGATTACTCACTTGCCCTGCGAACATGTTTATCATAGAGATTGCATTGTCAATTGGTTTGAGACGAGTAACTCGTGTCCCTTGTGCCGATCCGCAATGCCAACAATAGAGGAAGAAGAGGTGGCTCCGGATTGGTCTATAGTTCTCTCTGTGCTCACTGGTGGTATGTTAGCTGCTATGCTGGCCATGTTTGTTTCATGGAATCAATGCATAGGGAAAGGAAAGTCTTTACTTTCCTGTGTTTGGAAACCAAAGAAAGTTGATGGGAAAGAAAAGTGGTTCCTGGAGAGAAGGAAAATTGCATGGAATCTGCTTCCCTCCAACAACCCCAAGGATTCACTTTCCGTTCCAACTCCTGCAATTAATTTTAATATTGATGCTGTGTTTAAAAATGGAGCTGGTAGTTGGGGATTGTTGGTTCCAGATTCGGCTGGACTTGTTCTTGCAGGTTCTTCTGGCTATGTGCAGTCAGTTCAATCAGCCTCTGTGATGGAGGCTATTGCTGTTAGGAAGGCTTGTGCTATAGTGGAGTCTCTCAACTTGAGTCTGCAGATTTTAGCTACCAATCAAATGTCGGTTGGTACACAGTATCGGCAAAAAAAGTTGGTAACCGTACCGGTTCCAGTCCTAGATTTGATTGTTGATGCGATCTTGGAGTCTCCTTCCACTATGAAATTTGTAAAGTCGTGGTACCAAGCTGCAATGAGACCAGCACTAAGTGCCAAGGCTTATGCTCAAGGGCAGCCTTTGAGCTGTGCCATGTGTGCGGTCACACAACGCCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006355 GO:0006357 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007549 GO:0008150 GO:0008152 GO:0009048 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010498 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032446 GO:0036211 GO:0040029 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043433 GO:0043632 GO:0044092 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051090 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051603 GO:0060255 GO:0060816 GO:0061630 GO:0061659 GO:0065007 GO:0065009 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1900095 GO:1901564 GO:1901565 GO:1901575 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA zf-RING_2

Protein Analysis

353

Amino Acids

38.89

Weight (kDa)

5.65

Isoelectric Point (pI)

44.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	68 - 121	2e-09	Ring finger domain
zf-rbx1	PF12678	68 - 121	3.2e-09	RING-H2 zinc finger domain
zf-C3HC4_2	PF13923	92 - 120	1.1e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001335)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g21520 FvH4_5g06850 FvH4_5g06900 FvH4_5g06910 FvH4_5g06931 FvH4_5g06960 FvH4_5g06960 FvH4_5g06960 FvH4_5g06960 FvH4_5g07200 FvH4_5g07200 FvH4_5g07200 FvH4_5g07200 FvH4_5g07200 FvH4_5g08080 FvH4_5g08080 FvH4_5g08121 FvH4_5g09570 FvH4_5g09630
rosa_chinensis	RchiOBHm_Chr7g0189311 RchiOBHm_Chr7g0190371 RchiOBHm_Chr7g0191221 RchiOBHm_Chr7g0191551 RchiOBHm_Chr7g0191591 RchiOBHm_Chr7g0240901
rosa_multiflora	Rmu_co8454287.1_g000001 Rmu_co8455033.1_g000001 Rmu_co8484079.1_g000001 Rmu_sc0001123.1_g000020 Rmu_sc0001123.1_g000024 Rmu_sc0003634.1_g000006 Rmu_sc0003634.1_g000008 Rmu_sc0004860.1_g000013 Rmu_sc0004956.1_g000007 Rmu_sc0010599.1_g000010 Rmu_sc0029757.1_g000001
rosa_roxburghii	Rroxscaffold_157G00438370 Rroxscaffold_3G00263820
rosa_rugosa	Rorug06G0504200 Rorug06G0504300 Rorug06G0511100 Rorug07G0220400 Rorug07G0339900
rosa_samantha	Rh7AG102100 Rh7AG110000 Rh7AG119200 Rh7AG120500 Rh7DG113000 Rh7DG120200 Rh7DG122300 Rh7DG123700
rosa_wichuraiana	Rw7G010180 Rw7G010260 Rw7G010270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	9, 72
Acc36I	ACCTGC	1 cut(s)	710
Acc65I	GGTACC	1 cut(s)	963
AccB1I	GGYRCC	1 cut(s)	963
AccI	GTMKAC	1 cut(s)	246
AccIII	TCCGGA	1 cut(s)	400
AciI	CCGC	4 cut(s)	62, 79, 366, 1040
AclWI	GGATC	2 cut(s)	127, 357
AcoI	YGGCCR	2 cut(s)	63, 456
AcsI	RAATTY	1 cut(s)	947
AcyI	GRCGYC	2 cut(s)	6, 69
AdeI	CACNNNGTG	1 cut(s)	992
AfaI	GTAC	4 cut(s)	217, 856, 888, 965
AfiI	CCNNNNNNNGG	1 cut(s)	516
AflIII	ACRYGT	1 cut(s)	298
AgeI	ACCGGT	1 cut(s)	889
AgsI	TTSAA	1 cut(s)	749
AjnI	CCWGG	1 cut(s)	555
AloI	GAACNNNNNNTCC	2 cut(s)	675, 707
AluBI	AGCT	6 cut(s)	17, 446, 671, 833, 971, 1026
AluI	AGCT	6 cut(s)	17, 446, 671, 833, 971, 1026
Alw21I	GWGCWC	1 cut(s)	429
Alw26I	GTCTC	4 cut(s)	329, 810, 936, 973
AlwI	GGATC	2 cut(s)	127, 357
AlwNI	CAGNNNCTG	2 cut(s)	271, 758
Aor13HI	TCCGGA	1 cut(s)	400
AoxI	GGCC	3 cut(s)	63, 80, 456
ApeKI	GCWGC	5 cut(s)	22, 265, 446, 971, 1015
ApoI	RAATTY	1 cut(s)	947
AseI	ATTAAT	1 cut(s)	636
AsiGI	ACCGGT	1 cut(s)	889
Asp700I	GAANNNNTTC	1 cut(s)	255
Asp718I	GGTACC	1 cut(s)	963
AspA2I	CCTAGG	1 cut(s)	74
AsuII	TTCGAA	1 cut(s)	180
AvrII	CCTAGG	1 cut(s)	74
BalI	TGGCCA	1 cut(s)	458
BanI	GGYRCC	1 cut(s)	963
BanII	GRGCYC	1 cut(s)	225
BauI	CACGAG	1 cut(s)	346
Bbv12I	GWGCWC	1 cut(s)	429
BbvI	GCAGC	5 cut(s)	34, 277, 433, 958, 1027
BccI	CCATC	3 cut(s)	94, 530, 757
BcgI	CGANNNNNNTGC	2 cut(s)	170, 204
BciT130I	CCWGG	1 cut(s)	557
BclI	TGATCA	2 cut(s)	37, 229
BcoDI	GTCTC	4 cut(s)	329, 810, 936, 973
BfaI	CTAG	5 cut(s)	75, 146, 155, 192, 902
BfmI	CTRYAG	3 cut(s)	411, 795, 821
BfuAI	ACCTGC	1 cut(s)	710
BisI	GCNGC	7 cut(s)	23, 63, 80, 266, 447, 972, 1016
BlnI	CCTAGG	1 cut(s)	74
BlsI	GCNGC	7 cut(s)	24, 64, 81, 267, 448, 973, 1017
Bme1390I	CCNGG	1 cut(s)	557
BmiI	GGNNCC	5 cut(s)	399, 554, 693, 894, 965
BmrFI	CCNGG	1 cut(s)	557
BmsI	GCATC	2 cut(s)	640, 907
BpmI	CTGGAG	1 cut(s)	578
Bpu14I	TTCGAA	1 cut(s)	180
BpuEI	CTTGAG	2 cut(s)	835, 993
BsaHI	GRCGYC	2 cut(s)	6, 69
BsaI	GGTCTC	1 cut(s)	973
BsaJI	CCNNGG	3 cut(s)	74, 603, 996
BsaWI	WCCGGW	2 cut(s)	400, 889
Bsc4I	CCNNNNNNNGG	1 cut(s)	516
Bse118I	RCCGGY	1 cut(s)	889
Bse1I	ACTGG	2 cut(s)	436, 896
Bse3DI	GCAATG	2 cut(s)	375, 981
BseAI	TCCGGA	1 cut(s)	400
BseBI	CCWGG	1 cut(s)	557
BseDI	CCNNGG	3 cut(s)	74, 603, 996
BseGI	GGATG	1 cut(s)	123
BseLI	CCNNNNNNNGG	1 cut(s)	516
BseMI	GCAATG	2 cut(s)	375, 981
BseMII	CTCAG	1 cut(s)	127
BseNI	ACTGG	2 cut(s)	436, 896
BseX3I	CGGCCG	1 cut(s)	63
BseXI	GCAGC	5 cut(s)	34, 277, 433, 958, 1027
BsgI	GTGCAG	1 cut(s)	758
Bsh1285I	CGRYCG	1 cut(s)	66
BshFI	GGCC	3 cut(s)	65, 82, 458
BshNI	GGYRCC	1 cut(s)	963
BshTI	ACCGGT	1 cut(s)	889
BsiEI	CGRYCG	1 cut(s)	66
BsiHKAI	GWGCWC	1 cut(s)	429
BsiSI	CCGG	2 cut(s)	401, 890
BslFI	GGGAC	2 cut(s)	56, 336
BslI	CCNNNNNNNGG	1 cut(s)	516
BsmAI	GTCTC	4 cut(s)	329, 810, 936, 973
BsmBI	CGTCTC	1 cut(s)	329
BsmFI	GGGAC	2 cut(s)	56, 336
BsmI	GAATGC	1 cut(s)	255
BsnI	GGCC	3 cut(s)	65, 82, 458
Bso31I	GGTCTC	1 cut(s)	973
Bsp119I	TTCGAA	1 cut(s)	180
Bsp1286I	GDGCHC	2 cut(s)	225, 429
Bsp13I	TCCGGA	1 cut(s)	400
Bsp1407I	TGTACA	1 cut(s)	215
Bsp143I	GATC	5 cut(s)	37, 132, 229, 362, 921
BspACI	CCGC	4 cut(s)	62, 79, 366, 1040
BspANI	GGCC	3 cut(s)	65, 82, 458
BspCNI	CTCAG	1 cut(s)	126
BspEI	TCCGGA	1 cut(s)	400
BspHI	TCATGA	1 cut(s)	259
BspLI	GGNNCC	5 cut(s)	399, 554, 693, 894, 965
BspMAI	CTGCAG	1 cut(s)	825
BspMI	ACCTGC	1 cut(s)	710
BspPI	GGATC	2 cut(s)	127, 357
BspT104I	TTCGAA	1 cut(s)	180
BspT107I	GGYRCC	1 cut(s)	963
BspTNI	GGTCTC	1 cut(s)	973
BsrDI	GCAATG	2 cut(s)	375, 981
BsrFI	RCCGGY	1 cut(s)	889
BsrGI	TGTACA	1 cut(s)	215
BsrI	ACTGG	2 cut(s)	436, 896
BssAI	RCCGGY	1 cut(s)	889
BssECI	CCNNGG	3 cut(s)	74, 603, 996
BssMI	GATC	5 cut(s)	37, 132, 229, 362, 921
BssNI	GRCGYC	2 cut(s)	6, 69
BssSI	CACGAG	1 cut(s)	346
BssT1I	CCWWGG	3 cut(s)	74, 603, 996
Bst2BI	CACGAG	1 cut(s)	346
Bst2UI	CCWGG	1 cut(s)	557
Bst4CI	ACNGT	2 cut(s)	861, 886
Bst6I	CTCTTC	1 cut(s)	384
BstACI	GRCGYC	2 cut(s)	6, 69
BstAPI	GCANNNNNTGC	1 cut(s)	992
BstAUI	TGTACA	1 cut(s)	215
BstBI	TTCGAA	1 cut(s)	180
BstC8I	GCNNGC	1 cut(s)	456
BstDEI	CTNAG	2 cut(s)	113, 989
BstEII	GGTNACC	1 cut(s)	880
BstF5I	GGATG	1 cut(s)	123
BstKTI	GATC	5 cut(s)	40, 135, 232, 365, 924
BstMAI	GTCTC	4 cut(s)	329, 810, 936, 973
BstMBI	GATC	5 cut(s)	37, 132, 229, 362, 921
BstMCI	CGRYCG	1 cut(s)	66
BstMWI	GCNNNNNNNGC	3 cut(s)	455, 992, 1037
BstNI	CCWGG	1 cut(s)	557
BstNSI	RCATGY	2 cut(s)	207, 302
BstPI	GGTNACC	1 cut(s)	880
BstSCI	CCNGG	1 cut(s)	555
BstSFI	CTRYAG	3 cut(s)	411, 795, 821
BstV1I	GCAGC	5 cut(s)	34, 277, 433, 958, 1027
BstX2I	RGATCY	1 cut(s)	132
BstYI	RGATCY	1 cut(s)	132
BstZI	CGGCCG	1 cut(s)	63
BsuRI	GGCC	3 cut(s)	65, 82, 458
BtsCI	GGATG	1 cut(s)	123
BtsIMutI	CAGTG	1 cut(s)	429
BveI	ACCTGC	1 cut(s)	710
Cac8I	GCNNGC	1 cut(s)	456
CaiI	CAGNNNCTG	2 cut(s)	271, 758
CciI	TCATGA	1 cut(s)	259
Cfr10I	RCCGGY	1 cut(s)	889
Csp6I	GTAC	4 cut(s)	216, 855, 887, 964
CspAI	ACCGGT	1 cut(s)	889
CviAII	CATG	7 cut(s)	204, 260, 299, 460, 471, 576, 1033
CviQI	GTAC	4 cut(s)	216, 855, 887, 964
DdeI	CTNAG	2 cut(s)	113, 989
DpnI	GATC	5 cut(s)	39, 134, 231, 364, 923
DpnII	GATC	5 cut(s)	37, 132, 229, 362, 921
DraI	TTTAAA	1 cut(s)	661
DraIII	CACNNNGTG	1 cut(s)	992
EaeI	YGGCCR	2 cut(s)	63, 456
EagI	CGGCCG	1 cut(s)	63
Eam1104I	CTCTTC	1 cut(s)	384
EarI	CTCTTC	1 cut(s)	384
EclXI	CGGCCG	1 cut(s)	63
Eco130I	CCWWGG	3 cut(s)	74, 603, 996
Eco24I	GRGCYC	1 cut(s)	225
Eco31I	GGTCTC	1 cut(s)	973
Eco52I	CGGCCG	1 cut(s)	63
Eco91I	GGTNACC	1 cut(s)	880
EcoO65I	GGTNACC	1 cut(s)	880
EcoRII	CCWGG	1 cut(s)	555
EcoT14I	CCWWGG	3 cut(s)	74, 603, 996
EcoT22I	ATGCAT	1 cut(s)	485
EcoT38I	GRGCYC	1 cut(s)	225
ErhI	CCWWGG	3 cut(s)	74, 603, 996
Esp3I	CGTCTC	1 cut(s)	329
FaeI	CATG	7 cut(s)	207, 263, 302, 463, 474, 579, 1036
FalI	AAGNNNNNCTT	2 cut(s)	153, 185
FaqI	GGGAC	2 cut(s)	56, 336
FatI	CATG	7 cut(s)	203, 259, 298, 459, 470, 575, 1032
FbaI	TGATCA	2 cut(s)	37, 229
FblI	GTMKAC	1 cut(s)	246
Fnu4HI	GCNGC	7 cut(s)	23, 63, 80, 266, 447, 972, 1016
FokI	GGATG	1 cut(s)	130
FriOI	GRGCYC	1 cut(s)	225
Fsp4HI	GCNGC	7 cut(s)	23, 63, 80, 266, 447, 972, 1016
FspBI	CTAG	5 cut(s)	75, 146, 155, 192, 902
GluI	GCNGC	7 cut(s)	23, 63, 80, 266, 447, 972, 1016
GsuI	CTGGAG	1 cut(s)	578
HaeIII	GGCC	3 cut(s)	65, 82, 458
HapII	CCGG	2 cut(s)	401, 890
Hin1I	GRCGYC	2 cut(s)	6, 69
Hin1II	CATG	7 cut(s)	207, 263, 302, 463, 474, 579, 1036
HinfI	GANTC	7 cut(s)	475, 580, 608, 698, 803, 817, 929
HpaII	CCGG	2 cut(s)	401, 890
Hpy166II	GTNNAC	2 cut(s)	247, 857
Hpy188III	TCNNGA	6 cut(s)	97, 115, 146, 260, 401, 695
Hpy8I	GTNNAC	2 cut(s)	247, 857
Hpy99I	CGWCG	1 cut(s)	71
HpyAV	CCTTC	4 cut(s)	245, 558, 778, 945
HpyCH4III	ACNGT	2 cut(s)	861, 886
HpyCH4IV	ACGT	2 cut(s)	6, 69
HpyCH4V	TGCA	9 cut(s)	22, 318, 483, 575, 632, 719, 739, 823, 974
HpyF10VI	GCNNNNNNNGC	3 cut(s)	455, 992, 1037
HpyF3I	CTNAG	2 cut(s)	113, 989
HpySE526I	ACGT	2 cut(s)	6, 69
Hsp92I	GRCGYC	2 cut(s)	6, 69
Hsp92II	CATG	7 cut(s)	207, 263, 302, 463, 474, 579, 1036
Kpn2I	TCCGGA	1 cut(s)	400
KpnI	GGTACC	1 cut(s)	967
Ksp22I	TGATCA	2 cut(s)	37, 229
Kzo9I	GATC	5 cut(s)	37, 132, 229, 362, 921
LmnI	GCTCC	2 cut(s)	403, 668
Lsp1109I	GCAGC	5 cut(s)	34, 277, 433, 958, 1027
LweI	GCATC	2 cut(s)	640, 907
MaeI	CTAG	5 cut(s)	75, 146, 155, 192, 902
MaeII	ACGT	2 cut(s)	6, 69
MaeIII	GTNAC	3 cut(s)	341, 880, 1042
MalI	GATC	5 cut(s)	39, 134, 231, 364, 923
MboI	GATC	5 cut(s)	37, 132, 229, 362, 921
MboII	GAAGA	5 cut(s)	194, 209, 398, 401, 717
MfeI	CAATTG	1 cut(s)	325
MflI	RGATCY	1 cut(s)	132
MhlI	GDGCHC	2 cut(s)	225, 429
MlsI	TGGCCA	1 cut(s)	458
MluCI	AATT	5 cut(s)	325, 570, 633, 637, 947
MluNI	TGGCCA	1 cut(s)	458
MlyI	GAGTC	3 cut(s)	812, 826, 938
MmeI	TCCRAC	2 cut(s)	618, 647
MnlI	CCTC	8 cut(s)	52, 146, 159, 376, 385, 601, 760, 766
Mox20I	TGGCCA	1 cut(s)	458
Mph1103I	ATGCAT	1 cut(s)	485
MroI	TCCGGA	1 cut(s)	400
MroXI	GAANNNNTTC	1 cut(s)	255
MscI	TGGCCA	1 cut(s)	458
MseI	TTAA	4 cut(s)	636, 642, 660, 1057
Msp20I	TGGCCA	1 cut(s)	458
MspI	CCGG	2 cut(s)	401, 890
MspR9I	CCNGG	1 cut(s)	557
MunI	CAATTG	1 cut(s)	325
Mva1269I	GAATGC	1 cut(s)	255
MvaI	CCWGG	1 cut(s)	557
MwoI	GCNNNNNNNGC	3 cut(s)	455, 992, 1037
NdeII	GATC	5 cut(s)	37, 132, 229, 362, 921
NlaIII	CATG	7 cut(s)	207, 263, 302, 463, 474, 579, 1036
NlaIV	GGNNCC	5 cut(s)	399, 554, 693, 894, 965
NmuCI	GTSAC	1 cut(s)	1042
NsiI	ATGCAT	1 cut(s)	485
NspI	RCATGY	2 cut(s)	207, 302
NspV	TTCGAA	1 cut(s)	180
PagI	TCATGA	1 cut(s)	259
PciI	ACATGT	1 cut(s)	298
PcsI	WCGNNNNNNNCGW	2 cut(s)	63, 344
PctI	GAATGC	1 cut(s)	255
PdmI	GAANNNNTTC	1 cut(s)	255
PfeI	GAWTC	4 cut(s)	475, 580, 608, 698
PfoI	TCCNGGA	1 cut(s)	555
PinAI	ACCGGT	1 cut(s)	889
PkrI	GCNGC	7 cut(s)	24, 64, 81, 267, 448, 973, 1017
PleI	GAGTC	3 cut(s)	811, 825, 937
PpsI	GAGTC	3 cut(s)	811, 825, 937
PscI	ACATGT	1 cut(s)	298
PshBI	ATTAAT	1 cut(s)	636
Psp6I	CCWGG	1 cut(s)	555
PspEI	GGTNACC	1 cut(s)	880
PspGI	CCWGG	1 cut(s)	555
PspN4I	GGNNCC	5 cut(s)	399, 554, 693, 894, 965
PstI	CTGCAG	1 cut(s)	825
PstNI	CAGNNNCTG	2 cut(s)	271, 758
PsuI	RGATCY	1 cut(s)	132
RsaI	GTAC	4 cut(s)	217, 856, 888, 965
RsaNI	GTAC	4 cut(s)	216, 855, 887, 964
SaqAI	TTAA	4 cut(s)	636, 642, 660, 1057
SatI	GCNGC	7 cut(s)	23, 63, 80, 266, 447, 972, 1016
Sau3AI	GATC	5 cut(s)	37, 132, 229, 362, 921
SchI	GAGTC	3 cut(s)	812, 826, 938
ScrFI	CCNGG	1 cut(s)	557
SduI	GDGCHC	2 cut(s)	225, 429
SfaNI	GCATC	2 cut(s)	640, 907
SfcI	CTRYAG	3 cut(s)	411, 795, 821
SfuI	TTCGAA	1 cut(s)	180
SmlI	CTYRAG	2 cut(s)	814, 1008
SmoI	CTYRAG	2 cut(s)	814, 1008
Sse9I	AATT	5 cut(s)	325, 570, 633, 637, 947
SsiI	CCGC	4 cut(s)	62, 79, 366, 1040
SspI	AATATT	2 cut(s)	45, 646
SspMI	CTAG	5 cut(s)	75, 146, 155, 192, 902
StyD4I	CCNGG	1 cut(s)	555
StyI	CCWWGG	3 cut(s)	74, 603, 996
TaaI	ACNGT	2 cut(s)	861, 886
TaiI	ACGT	2 cut(s)	9, 72
TaqI	TCGA	2 cut(s)	57, 180
TasI	AATT	5 cut(s)	325, 570, 633, 637, 947
TatI	WGTACW	1 cut(s)	215
TauI	GCSGC	2 cut(s)	65, 82
TfiI	GAWTC	4 cut(s)	475, 580, 608, 698
Tru1I	TTAA	4 cut(s)	636, 642, 660, 1057
Tru9I	TTAA	4 cut(s)	636, 642, 660, 1057
TscAI	CASTG	1 cut(s)	436
TseFI	GTSAC	1 cut(s)	1042
TseI	GCWGC	5 cut(s)	22, 265, 446, 971, 1015
Tsp45I	GTSAC	1 cut(s)	1042
TspDTI	ATGAA	3 cut(s)	248, 459, 959
TspGWI	ACGGA	1 cut(s)	607
TspRI	CASTG	1 cut(s)	436
VspI	ATTAAT	1 cut(s)	636
XapI	RAATTY	1 cut(s)	947
XbaI	TCTAGA	1 cut(s)	145
XceI	RCATGY	2 cut(s)	207, 302
XmaJI	CCTAGG	1 cut(s)	74
XmiI	GTMKAC	1 cut(s)	246
XmnI	GAANNNNTTC	1 cut(s)	255
XspI	CTAG	5 cut(s)	75, 146, 155, 192, 902
ZraI	GACGTC	2 cut(s)	7, 70
Zsp2I	ATGCAT	1 cut(s)	485

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.