FvH4_5g13640

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001329
Physical Location & Seq
Forward (+)
7714777 .. 7718924
4148 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g13640.t3

Sequence Viewer

Length: 1041 bp
ATGCAGAGGCTTCTTTCTGTGAAATTAGCCTCAAACCTCACCAAAAACCTCTCAACCACCACCTCCACCACCACCACCACCACCACAAGCTCTTCCTTTTTCCACTTCCAACCTCACAAAACCTTCTCTTTCACCAAACCACCCCAAACCCAGTCCCAAAATCACTTCTTTAGCTCCTTCCCCACACACCCACTTCCCCATCAGCACAATCTCACTCACAAAATTCATGGCTTTCTCTCCAACCCAATAATCAGGAGGCACTTTTTGCTGGGTTCCGCAAATACCCTTTTGAGGGTATCAAGCAAGAGACTTTCGGATTGCAGGCTTGCCTTTCTCCAACGCAAATTTCCCAGACAGAGTTTTGACTTGAACCCAAGTCGCATTTCTAATCAGAGTGCCTGGAGATCATGGTTGGGAAGGTTATCAAGCAGTGACATGGTTTTGGGATTGGTTATAGCTAATGTTGCTGTTTTTCTTTTATGGAAGATAGCAGACCCTGTATTCATGTACAAGAATTTTACTCTCTCAGTATACAATGTTACAAGTGGGCGTCTTCACACATTGATAACTTCTGCATTTAGTCACATTGATGTTGGGCATATCATTTCTAACATGATTGGACTGTATTTTTTTGGGATCAATATTGGAAGAACATTCGGGCCTGAGTTTTTGCTAAAGTTGTATCTAGCTGGAGCTATTGGTGGCTCAGTCTTTTACTTGGTGCACCATGCCTTCCTGGCTGCATCATCAAAGAACCGACCATTTGGGATGATGGATGCTTCTAAGACTCCAGCTTTGGGGGCAAGCGGTGCCGTTAATGCTATCATGTTGCTTGATATATTTCTTCACCCCACAGCTACTCTTTACTTAGAGTTTATCATACCAGTTCCTGCCATGCTGCTGGGGATCTTTCTAATTGGAAAGGATATCTTAAGGATAATGGAGGGAAACAGTCAGATTTCAGGAGCTGCACACTTGGGCGGTGCGGCAGTAGCAGCCATAGCCTGGGCAAGACTTCGAAAGGGGCGTCGTTTATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

347

Amino Acids

38.33

Weight (kDa)

11.01

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 183 - 306 6.8e-20 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 809
AccB7I CCANNNNNTGG 1 cut(s) 1005
AccI GTMKAC 1 cut(s) 531
AciI CCGC 4 cut(s) 276, 807, 981, 986
AclWI GGATC 2 cut(s) 644, 914
AcsI RAATTY 3 cut(s) 222, 344, 514
AcyI GRCGYC 2 cut(s) 550, 1027
AfaI GTAC 1 cut(s) 509
AfiI CCNNNNNNNGG 4 cut(s) 291, 292, 797, 1005
AflII CTTAAG 1 cut(s) 931
AgsI TTSAA 1 cut(s) 370
AjnI CCWGG 3 cut(s) 398, 735, 1004
AjuI GAANNNNNNNTTGG 4 cut(s) 330, 362, 367, 399
AluBI AGCT 8 cut(s) 90, 174, 458, 689, 695, 794, 857, 968
AluI AGCT 8 cut(s) 90, 174, 458, 689, 695, 794, 857, 968
Alw21I GWGCWC 1 cut(s) 726
Alw26I GTCTC 1 cut(s) 301
Alw44I GTGCAC 1 cut(s) 722
AlwI GGATC 2 cut(s) 644, 914
AlwNI CAGNNNCTG 3 cut(s) 497, 890, 968
AoxI GGCC 1 cut(s) 659
ApaLI GTGCAC 1 cut(s) 722
ApeKI GCWGC 4 cut(s) 740, 898, 968, 995
ApoI RAATTY 3 cut(s) 222, 344, 514
ArsI GACNNNNNNTTYG 3 cut(s) 778, 810, 1012
AspS9I GGNCC 1 cut(s) 659
AsuHPI GGTGA 3 cut(s) 31, 124, 839
AsuII TTCGAA 1 cut(s) 1018
BaeGI GKGCMC 1 cut(s) 726
BanI GGYRCC 1 cut(s) 809
BbsI GAAGAC 1 cut(s) 545
Bbv12I GWGCWC 1 cut(s) 726
BbvI GCAGC 4 cut(s) 727, 885, 955, 1007
BccI CCATC 2 cut(s) 207, 766
BceAI ACGGC 1 cut(s) 797
BciT130I CCWGG 3 cut(s) 400, 737, 1006
BcoDI GTCTC 1 cut(s) 301
BfaI CTAG 2 cut(s) 686, 1039
BfrI CTTAAG 1 cut(s) 931
BglI GCCNNNNNGGC 1 cut(s) 737
BisI GCNGC 5 cut(s) 741, 899, 969, 987, 996
BlsI GCNGC 5 cut(s) 742, 900, 970, 988, 997
Bme1390I CCNGG 3 cut(s) 400, 737, 1006
BmgT120I GGNCC 1 cut(s) 659
BmiI GGNNCC 2 cut(s) 274, 811
BmrFI CCNGG 3 cut(s) 400, 737, 1006
BmrI ACTGGG 1 cut(s) 145
BmsI GCATC 2 cut(s) 752, 766
BmuI ACTGGG 1 cut(s) 145
BpiI GAAGAC 1 cut(s) 545
BpmI CTGGAG 3 cut(s) 421, 711, 774
Bpu14I TTCGAA 1 cut(s) 1018
BsaHI GRCGYC 2 cut(s) 550, 1027
BsaJI CCNNGG 1 cut(s) 1005
Bsc4I CCNNNNNNNGG 4 cut(s) 291, 292, 797, 1005
Bse1I ACTGG 2 cut(s) 151, 884
BseBI CCWGG 3 cut(s) 400, 737, 1006
BseDI CCNNGG 1 cut(s) 1005
BseGI GGATG 2 cut(s) 774, 781
BseLI CCNNNNNNNGG 4 cut(s) 291, 292, 797, 1005
BseMII CTCAG 3 cut(s) 540, 654, 720
BseNI ACTGG 2 cut(s) 151, 884
BseSI GKGCMC 1 cut(s) 726
BseXI GCAGC 4 cut(s) 727, 885, 955, 1007
BseYI CCCAGC 2 cut(s) 268, 901
BsgI GTGCAG 1 cut(s) 954
BshFI GGCC 1 cut(s) 661
BshNI GGYRCC 1 cut(s) 809
BsiHKAI GWGCWC 1 cut(s) 726
BslFI GGGAC 1 cut(s) 139
BslI CCNNNNNNNGG 4 cut(s) 291, 292, 797, 1005
BsmAI GTCTC 1 cut(s) 301
BsmFI GGGAC 1 cut(s) 139
BsnI GGCC 1 cut(s) 661
Bsp119I TTCGAA 1 cut(s) 1018
Bsp1286I GDGCHC 1 cut(s) 726
Bsp1407I TGTACA 1 cut(s) 507
Bsp143I GATC 3 cut(s) 404, 636, 906
BspACI CCGC 4 cut(s) 276, 807, 981, 986
BspANI GGCC 1 cut(s) 661
BspCNI CTCAG 3 cut(s) 539, 655, 719
BspLI GGNNCC 2 cut(s) 274, 811
BspPI GGATC 2 cut(s) 644, 914
BspQI GCTCTTC 1 cut(s) 97
BspT104I TTCGAA 1 cut(s) 1018
BspT107I GGYRCC 1 cut(s) 809
BspTI CTTAAG 1 cut(s) 931
BsrGI TGTACA 1 cut(s) 507
BsrI ACTGG 2 cut(s) 151, 884
BssECI CCNNGG 1 cut(s) 1005
BssMI GATC 3 cut(s) 404, 636, 906
BssNAI GTATAC 1 cut(s) 532
BssNI GRCGYC 2 cut(s) 550, 1027
Bst1107I GTATAC 1 cut(s) 532
Bst2UI CCWGG 3 cut(s) 400, 737, 1006
Bst4CI ACNGT 2 cut(s) 624, 953
Bst6I CTCTTC 1 cut(s) 97
BstACI GRCGYC 2 cut(s) 550, 1027
BstAFI CTTAAG 1 cut(s) 931
BstAPI GCANNNNNTGC 2 cut(s) 265, 809
BstAUI TGTACA 1 cut(s) 507
BstBI TTCGAA 1 cut(s) 1018
BstC8I GCNNGC 3 cut(s) 323, 327, 805
BstDEI CTNAG 5 cut(s) 526, 663, 706, 783, 868
BstF5I GGATG 2 cut(s) 774, 781
BstKTI GATC 3 cut(s) 407, 639, 909
BstMAI GTCTC 1 cut(s) 301
BstMBI GATC 3 cut(s) 404, 636, 906
BstMWI GCNNNNNNNGC 9 cut(s) 265, 464, 737, 800, 809, 818, 992, 995, 1001
BstNI CCWGG 3 cut(s) 400, 737, 1006
BstSCI CCNGG 3 cut(s) 398, 735, 1004
BstSLI GKGCMC 1 cut(s) 726
BstV1I GCAGC 4 cut(s) 727, 885, 955, 1007
BstV2I GAAGAC 1 cut(s) 545
BstX2I RGATCY 1 cut(s) 906
BstXI CCANNNNNNTGG 1 cut(s) 901
BstYI RGATCY 1 cut(s) 906
BstZ17I GTATAC 1 cut(s) 532
BsuRI GGCC 1 cut(s) 661
BtsCI GGATG 2 cut(s) 774, 781
BtsI GCAGTG 1 cut(s) 436
BtsIMutI CAGTG 1 cut(s) 436
Cac8I GCNNGC 3 cut(s) 323, 327, 805
CaiI CAGNNNCTG 3 cut(s) 497, 890, 968
Cfr13I GGNCC 1 cut(s) 659
CseI GACGC 2 cut(s) 539, 1016
Csp6I GTAC 1 cut(s) 508
CviAII CATG 8 cut(s) 227, 408, 436, 505, 613, 728, 826, 895
CviQI GTAC 1 cut(s) 508
DdeI CTNAG 5 cut(s) 526, 663, 706, 783, 868
DpnI GATC 3 cut(s) 406, 638, 908
DpnII GATC 3 cut(s) 404, 636, 906
Eam1104I CTCTTC 1 cut(s) 97
EarI CTCTTC 1 cut(s) 97
Eco32I GATATC 1 cut(s) 928
EcoRII CCWGG 3 cut(s) 398, 735, 1004
EcoRV GATATC 1 cut(s) 928
FaeI CATG 8 cut(s) 230, 411, 439, 508, 616, 731, 829, 898
FalI AAGNNNNNCTT 2 cut(s) 914, 946
FaqI GGGAC 1 cut(s) 139
FatI CATG 8 cut(s) 226, 407, 435, 504, 612, 727, 825, 894
FblI GTMKAC 1 cut(s) 531
Fnu4HI GCNGC 5 cut(s) 741, 899, 969, 987, 996
FokI GGATG 2 cut(s) 781, 788
Fsp4HI GCNGC 5 cut(s) 741, 899, 969, 987, 996
FspBI CTAG 2 cut(s) 686, 1039
GluI GCNGC 5 cut(s) 741, 899, 969, 987, 996
GsaI CCCAGC 2 cut(s) 272, 905
GsuI CTGGAG 3 cut(s) 421, 711, 774
HaeIII GGCC 1 cut(s) 661
HgaI GACGC 2 cut(s) 539, 1016
Hin1I GRCGYC 2 cut(s) 550, 1027
Hin1II CATG 8 cut(s) 230, 411, 439, 508, 616, 731, 829, 898
HinfI GANTC 1 cut(s) 787
HphI GGTGA 3 cut(s) 31, 124, 839
Hpy166II GTNNAC 2 cut(s) 532, 724
Hpy188I TCNGA 3 cut(s) 316, 393, 957
Hpy188III TCNNGA 2 cut(s) 253, 963
Hpy8I GTNNAC 2 cut(s) 532, 724
Hpy99I CGWCG 1 cut(s) 1032
HpyAV CCTTC 4 cut(s) 133, 187, 411, 742
HpyCH4III ACNGT 2 cut(s) 624, 953
HpyCH4V TGCA 6 cut(s) 4, 321, 575, 724, 743, 971
HpyF10VI GCNNNNNNNGC 9 cut(s) 265, 464, 737, 800, 809, 818, 992, 995, 1001
HpyF3I CTNAG 5 cut(s) 526, 663, 706, 783, 868
Hsp92I GRCGYC 2 cut(s) 550, 1027
Hsp92II CATG 8 cut(s) 230, 411, 439, 508, 616, 731, 829, 898
Kzo9I GATC 3 cut(s) 404, 636, 906
LguI GCTCTTC 1 cut(s) 97
LmnI GCTCC 3 cut(s) 179, 692, 965
Lsp1109I GCAGC 4 cut(s) 727, 885, 955, 1007
LweI GCATC 2 cut(s) 752, 766
MaeI CTAG 2 cut(s) 686, 1039
MaeIII GTNAC 3 cut(s) 431, 538, 581
MalI GATC 3 cut(s) 406, 638, 908
MboI GATC 3 cut(s) 404, 636, 906
MboII GAAGA 5 cut(s) 84, 496, 545, 660, 836
MflI RGATCY 1 cut(s) 906
MhlI GDGCHC 1 cut(s) 726
MluCI AATT 5 cut(s) 23, 222, 344, 514, 915
MlyI GAGTC 1 cut(s) 781
MmeI TCCRAC 3 cut(s) 133, 264, 361
MnlI CCTC 8 cut(s) 40, 47, 59, 73, 123, 249, 285, 937
MseI TTAA 2 cut(s) 816, 932
MslI CAYNNNNRTG 1 cut(s) 588
MspCI CTTAAG 1 cut(s) 931
MspR9I CCNGG 3 cut(s) 400, 737, 1006
MvaI CCWGG 3 cut(s) 400, 737, 1006
MwoI GCNNNNNNNGC 9 cut(s) 265, 464, 737, 800, 809, 818, 992, 995, 1001
NdeII GATC 3 cut(s) 404, 636, 906
NlaIII CATG 8 cut(s) 230, 411, 439, 508, 616, 731, 829, 898
NlaIV GGNNCC 2 cut(s) 274, 811
NmuCI GTSAC 2 cut(s) 431, 581
NspV TTCGAA 1 cut(s) 1018
PciSI GCTCTTC 1 cut(s) 97
PcsI WCGNNNNNNNCGW 1 cut(s) 1024
PflMI CCANNNNNTGG 1 cut(s) 1005
PkrI GCNGC 5 cut(s) 742, 900, 970, 988, 997
PleI GAGTC 1 cut(s) 781
PpsI GAGTC 1 cut(s) 781
Psp6I CCWGG 3 cut(s) 398, 735, 1004
PspFI CCCAGC 2 cut(s) 268, 901
PspGI CCWGG 3 cut(s) 398, 735, 1004
PspN4I GGNNCC 2 cut(s) 274, 811
PspPI GGNCC 1 cut(s) 659
PstNI CAGNNNCTG 3 cut(s) 497, 890, 968
PsuI RGATCY 1 cut(s) 906
RsaI GTAC 1 cut(s) 509
RsaNI GTAC 1 cut(s) 508
RseI CAYNNNNRTG 1 cut(s) 588
SapI GCTCTTC 1 cut(s) 97
SaqAI TTAA 2 cut(s) 816, 932
SatI GCNGC 5 cut(s) 741, 899, 969, 987, 996
Sau3AI GATC 3 cut(s) 404, 636, 906
Sau96I GGNCC 1 cut(s) 659
SchI GAGTC 1 cut(s) 781
ScrFI CCNGG 3 cut(s) 400, 737, 1006
SduI GDGCHC 1 cut(s) 726
SfaNI GCATC 2 cut(s) 752, 766
SfuI TTCGAA 1 cut(s) 1018
SmiMI CAYNNNNRTG 1 cut(s) 588
SmlI CTYRAG 1 cut(s) 931
SmoI CTYRAG 1 cut(s) 931
Sse9I AATT 5 cut(s) 23, 222, 344, 514, 915
SsiI CCGC 4 cut(s) 276, 807, 981, 986
SspI AATATT 1 cut(s) 643
SspMI CTAG 2 cut(s) 686, 1039
StyD4I CCNGG 3 cut(s) 398, 735, 1004
TaaI ACNGT 2 cut(s) 624, 953
TaqI TCGA 1 cut(s) 1018
TasI AATT 5 cut(s) 23, 222, 344, 514, 915
TatI WGTACW 1 cut(s) 507
TauI GCSGC 1 cut(s) 989
Tru1I TTAA 2 cut(s) 816, 932
Tru9I TTAA 2 cut(s) 816, 932
TscAI CASTG 1 cut(s) 436
TseFI GTSAC 2 cut(s) 431, 581
TseI GCWGC 4 cut(s) 740, 898, 968, 995
Tsp45I GTSAC 2 cut(s) 431, 581
TspDTI ATGAA 2 cut(s) 215, 493
TspRI CASTG 1 cut(s) 436
Van91I CCANNNNNTGG 1 cut(s) 1005
Vha464I CTTAAG 1 cut(s) 931
VneI GTGCAC 1 cut(s) 722
XapI RAATTY 3 cut(s) 222, 344, 514
XmiI GTMKAC 1 cut(s) 531
XspI CTAG 2 cut(s) 686, 1039
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.