RchiOBHm_Chr7g0183321

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001329
Physical Location & Seq
Reverse (-)
4252543 .. 4257002
4460 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16355

Sequence Viewer

Length: 1026 bp
ATGCAGAGGATTGTTTCTGTGAAATTAGCCTCAAACCTCCCCAAAAACCTATCAACCTCCACCACCACCCAAAGCTCTTCCCTTCTCCACTTCCAACCTCACAAAACCTTCTCTCTCACGAAACCACCCCAAACCCAGACCCAAAATCACTTCTTTAGCTCCTTCCCCACAAACCCACTTCCCCATCAGCACAATCTCACTCACAAAATTCATGGCTTTCTCTCCAACCCAGTACTCAGAAAACACTTTTCTTTGGATTCCGCAAATACCCTTTTGAGGGTATCGAGCAAGAGGCTTTCGGATTGCAGGCTTGCCTTTCTCCAAAGCAAGTTTCCCAAACAGAGTTTTAACTTCAACCCAAGTCGTATTTCTAATCAGAGTGCCTGGAGAACATGGTTGCGAAGGTTATCAAGCGGTGACATGGTTTTGGGATTGGTTGTAGCTAATGTTGCTGTTTTTCTTTTATGGAGGATAGCAGACCCTATATTCATGTACAAGAATTTTACTATCTCATTAAACAATTTTACAAGTGGACGTCTTCACACATTGATAACTTCTGCATTTAGTCACGTTGATATTTGGCATATCATTTCTAACATGATTGGACTGTATTTTTTTGGGATCAATATTGGAAGAACTTTTGGGCCTGAGTTTTTGCTGAAGTTGTATCTAGCTGGAGCTATTGGTGGCTCAGTCTTTTACTTGGTGCACCATGCCTTCCTGGCTGCATCATCGAAGAATCGACCATTTGGGATTATGGACGCTTCCAAGGCCCCAGGATTGGGGGCAAGCGGTGCTGTTAATGCTATCATGTTGCTTGATATATTCCTTCACCCCACAGCTACCCTCTACTTTGATTTTATCATACCAGTTCCTGCCATGCTGCTGGGAATCTTTCTAATTGGAAAGGATATCTTAAGAATAATGGAGGGAAACAGTCAGATCTCAGGATCTGCACACTTGGGCGGTGCAGCAGTAGCAGCCATAGCCTGGGCAAGACTTCGAAAGGGGCGTGGTTTATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

341

Amino Acids

37.78

Weight (kDa)

10.9

Isoelectric Point (pI)

35.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 178 - 331 1.9e-25 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 538
AccB7I CCANNNNNTGG 1 cut(s) 990
AciI CCGC 4 cut(s) 261, 414, 792, 966
AclWI GGATC 2 cut(s) 629, 958
AcsI RAATTY 2 cut(s) 207, 499
AcuI CTGAAG 1 cut(s) 680
AcyI GRCGYC 1 cut(s) 535
AfaI GTAC 2 cut(s) 234, 494
AfiI CCNNNNNNNGG 5 cut(s) 276, 277, 781, 782, 990
AflII CTTAAG 1 cut(s) 916
AgsI TTSAA 1 cut(s) 355
AjnI CCWGG 4 cut(s) 383, 720, 775, 989
AjuI GAANNNNNNNTTGG 4 cut(s) 315, 347, 352, 384
AluBI AGCT 6 cut(s) 75, 159, 443, 674, 680, 842
AluI AGCT 6 cut(s) 75, 159, 443, 674, 680, 842
Alw21I GWGCWC 1 cut(s) 711
Alw44I GTGCAC 1 cut(s) 707
AlwI GGATC 2 cut(s) 629, 958
AlwNI CAGNNNCTG 2 cut(s) 875, 953
AoxI GGCC 2 cut(s) 644, 771
ApaLI GTGCAC 1 cut(s) 707
ApeKI GCWGC 4 cut(s) 725, 883, 971, 980
ApoI RAATTY 2 cut(s) 207, 499
AspS9I GGNCC 2 cut(s) 644, 772
AsuHPI GGTGA 2 cut(s) 428, 824
AsuII TTCGAA 1 cut(s) 1003
BaeGI GKGCMC 1 cut(s) 711
BbsI GAAGAC 1 cut(s) 530
Bbv12I GWGCWC 1 cut(s) 711
BbvI GCAGC 4 cut(s) 712, 870, 983, 992
BccI CCATC 1 cut(s) 192
BciT130I CCWGG 4 cut(s) 385, 722, 777, 991
BfaI CTAG 2 cut(s) 671, 1024
BfrI CTTAAG 1 cut(s) 916
BglI GCCNNNNNGGC 1 cut(s) 722
BglII AGATCT 1 cut(s) 942
BisI GCNGC 4 cut(s) 726, 884, 972, 981
BlsI GCNGC 4 cut(s) 727, 885, 973, 982
BmcAI AGTACT 1 cut(s) 234
Bme1390I CCNGG 4 cut(s) 385, 722, 777, 991
BmgT120I GGNCC 2 cut(s) 644, 772
BmiI GGNNCC 1 cut(s) 774
BmrFI CCNGG 4 cut(s) 385, 722, 777, 991
BmrI ACTGGG 1 cut(s) 224
BmsI GCATC 1 cut(s) 737
BmuI ACTGGG 1 cut(s) 224
BpiI GAAGAC 1 cut(s) 530
BpmI CTGGAG 2 cut(s) 406, 696
Bpu14I TTCGAA 1 cut(s) 1003
BsaHI GRCGYC 1 cut(s) 535
BsaJI CCNNGG 3 cut(s) 768, 775, 990
Bsc4I CCNNNNNNNGG 5 cut(s) 276, 277, 781, 782, 990
Bse1I ACTGG 2 cut(s) 230, 869
BseBI CCWGG 4 cut(s) 385, 722, 777, 991
BseDI CCNNGG 3 cut(s) 768, 775, 990
BseLI CCNNNNNNNGG 5 cut(s) 276, 277, 781, 782, 990
BseMII CTCAG 4 cut(s) 250, 639, 705, 960
BseNI ACTGG 2 cut(s) 230, 869
BseSI GKGCMC 1 cut(s) 711
BseXI GCAGC 4 cut(s) 712, 870, 983, 992
BseYI CCCAGC 1 cut(s) 886
BsgI GTGCAG 2 cut(s) 939, 990
BshFI GGCC 2 cut(s) 646, 773
BsiHKAI GWGCWC 1 cut(s) 711
BslI CCNNNNNNNGG 5 cut(s) 276, 277, 781, 782, 990
BsnI GGCC 2 cut(s) 646, 773
Bsp119I TTCGAA 1 cut(s) 1003
Bsp1286I GDGCHC 1 cut(s) 711
Bsp1407I TGTACA 1 cut(s) 492
Bsp143I GATC 3 cut(s) 621, 942, 950
BspACI CCGC 4 cut(s) 261, 414, 792, 966
BspANI GGCC 2 cut(s) 646, 773
BspCNI CTCAG 4 cut(s) 249, 640, 704, 959
BspLI GGNNCC 1 cut(s) 774
BspPI GGATC 2 cut(s) 629, 958
BspQI GCTCTTC 1 cut(s) 82
BspT104I TTCGAA 1 cut(s) 1003
BspTI CTTAAG 1 cut(s) 916
BsrGI TGTACA 1 cut(s) 492
BsrI ACTGG 2 cut(s) 230, 869
BssECI CCNNGG 3 cut(s) 768, 775, 990
BssMI GATC 3 cut(s) 621, 942, 950
BssNI GRCGYC 1 cut(s) 535
BssT1I CCWWGG 1 cut(s) 768
Bst2UI CCWGG 4 cut(s) 385, 722, 777, 991
Bst4CI ACNGT 2 cut(s) 609, 938
Bst6I CTCTTC 1 cut(s) 82
BstACI GRCGYC 1 cut(s) 535
BstAFI CTTAAG 1 cut(s) 916
BstAPI GCANNNNNTGC 1 cut(s) 794
BstAUI TGTACA 1 cut(s) 492
BstBI TTCGAA 1 cut(s) 1003
BstC8I GCNNGC 3 cut(s) 308, 312, 790
BstDEI CTNAG 4 cut(s) 236, 648, 691, 946
BstKTI GATC 3 cut(s) 624, 945, 953
BstMBI GATC 3 cut(s) 621, 942, 950
BstMWI GCNNNNNNNGC 8 cut(s) 449, 722, 770, 794, 803, 977, 980, 986
BstNI CCWGG 4 cut(s) 385, 722, 777, 991
BstSCI CCNGG 4 cut(s) 383, 720, 775, 989
BstSLI GKGCMC 1 cut(s) 711
BstV1I GCAGC 4 cut(s) 712, 870, 983, 992
BstV2I GAAGAC 1 cut(s) 530
BstX2I RGATCY 2 cut(s) 942, 950
BstXI CCANNNNNNTGG 1 cut(s) 886
BstYI RGATCY 2 cut(s) 942, 950
BsuRI GGCC 2 cut(s) 646, 773
Cac8I GCNNGC 3 cut(s) 308, 312, 790
CaiI CAGNNNCTG 2 cut(s) 875, 953
Cfr13I GGNCC 2 cut(s) 644, 772
CseI GACGC 1 cut(s) 770
Csp6I GTAC 2 cut(s) 233, 493
CviAII CATG 8 cut(s) 212, 393, 421, 490, 598, 713, 811, 880
CviQI GTAC 2 cut(s) 233, 493
DdeI CTNAG 4 cut(s) 236, 648, 691, 946
DpnI GATC 3 cut(s) 623, 944, 952
DpnII GATC 3 cut(s) 621, 942, 950
Eam1104I CTCTTC 1 cut(s) 82
EarI CTCTTC 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 768
Eco32I GATATC 1 cut(s) 913
Eco57I CTGAAG 1 cut(s) 680
EcoO109I RGGNCCY 1 cut(s) 772
EcoRII CCWGG 4 cut(s) 383, 720, 775, 989
EcoRV GATATC 1 cut(s) 913
EcoT14I CCWWGG 1 cut(s) 768
ErhI CCWWGG 1 cut(s) 768
FaeI CATG 8 cut(s) 215, 396, 424, 493, 601, 716, 814, 883
FalI AAGNNNNNCTT 2 cut(s) 899, 931
FatI CATG 8 cut(s) 211, 392, 420, 489, 597, 712, 810, 879
Fnu4HI GCNGC 4 cut(s) 726, 884, 972, 981
Fsp4HI GCNGC 4 cut(s) 726, 884, 972, 981
FspBI CTAG 2 cut(s) 671, 1024
GluI GCNGC 4 cut(s) 726, 884, 972, 981
GsaI CCCAGC 1 cut(s) 890
GsuI CTGGAG 2 cut(s) 406, 696
HaeIII GGCC 2 cut(s) 646, 773
HgaI GACGC 1 cut(s) 770
Hin1I GRCGYC 1 cut(s) 535
Hin1II CATG 8 cut(s) 215, 396, 424, 493, 601, 716, 814, 883
HinfI GANTC 3 cut(s) 257, 739, 891
HphI GGTGA 2 cut(s) 428, 824
Hpy166II GTNNAC 2 cut(s) 533, 709
Hpy188I TCNGA 4 cut(s) 239, 301, 378, 942
Hpy188III TCNNGA 2 cut(s) 118, 948
Hpy8I GTNNAC 2 cut(s) 533, 709
HpyAV CCTTC 6 cut(s) 92, 118, 172, 396, 727, 839
HpyCH4III ACNGT 2 cut(s) 609, 938
HpyCH4IV ACGT 2 cut(s) 535, 570
HpyCH4V TGCA 7 cut(s) 4, 306, 560, 709, 728, 956, 971
HpyF10VI GCNNNNNNNGC 8 cut(s) 449, 722, 770, 794, 803, 977, 980, 986
HpyF3I CTNAG 4 cut(s) 236, 648, 691, 946
HpySE526I ACGT 2 cut(s) 535, 570
Hsp92I GRCGYC 1 cut(s) 535
Hsp92II CATG 8 cut(s) 215, 396, 424, 493, 601, 716, 814, 883
Kzo9I GATC 3 cut(s) 621, 942, 950
LguI GCTCTTC 1 cut(s) 82
LmnI GCTCC 2 cut(s) 164, 677
Lsp1109I GCAGC 4 cut(s) 712, 870, 983, 992
LweI GCATC 1 cut(s) 737
MaeI CTAG 2 cut(s) 671, 1024
MaeII ACGT 2 cut(s) 535, 570
MaeIII GTNAC 2 cut(s) 416, 566
MalI GATC 3 cut(s) 623, 944, 952
MboI GATC 3 cut(s) 621, 942, 950
MboII GAAGA 4 cut(s) 69, 530, 645, 748
MflI RGATCY 2 cut(s) 942, 950
MhlI GDGCHC 1 cut(s) 711
MluCI AATT 5 cut(s) 23, 207, 499, 520, 900
MmeI TCCRAC 2 cut(s) 118, 249
MnlI CCTC 9 cut(s) 40, 47, 67, 108, 270, 285, 462, 857, 922
MseI TTAA 4 cut(s) 348, 515, 801, 917
MspCI CTTAAG 1 cut(s) 916
MspR9I CCNGG 4 cut(s) 385, 722, 777, 991
MvaI CCWGG 4 cut(s) 385, 722, 777, 991
MwoI GCNNNNNNNGC 8 cut(s) 449, 722, 770, 794, 803, 977, 980, 986
NdeII GATC 3 cut(s) 621, 942, 950
NlaIII CATG 8 cut(s) 215, 396, 424, 493, 601, 716, 814, 883
NlaIV GGNNCC 1 cut(s) 774
NmuCI GTSAC 2 cut(s) 416, 566
NspV TTCGAA 1 cut(s) 1003
PciSI GCTCTTC 1 cut(s) 82
PcsI WCGNNNNNNNCGW 1 cut(s) 1009
PfeI GAWTC 3 cut(s) 257, 739, 891
PflMI CCANNNNNTGG 1 cut(s) 990
PkrI GCNGC 4 cut(s) 727, 885, 973, 982
Psp6I CCWGG 4 cut(s) 383, 720, 775, 989
PspFI CCCAGC 1 cut(s) 886
PspGI CCWGG 4 cut(s) 383, 720, 775, 989
PspN4I GGNNCC 1 cut(s) 774
PspPI GGNCC 2 cut(s) 644, 772
PstNI CAGNNNCTG 2 cut(s) 875, 953
PsuI RGATCY 2 cut(s) 942, 950
RsaI GTAC 2 cut(s) 234, 494
RsaNI GTAC 2 cut(s) 233, 493
SapI GCTCTTC 1 cut(s) 82
SaqAI TTAA 4 cut(s) 348, 515, 801, 917
SatI GCNGC 4 cut(s) 726, 884, 972, 981
Sau3AI GATC 3 cut(s) 621, 942, 950
Sau96I GGNCC 2 cut(s) 644, 772
ScaI AGTACT 1 cut(s) 234
ScrFI CCNGG 4 cut(s) 385, 722, 777, 991
SduI GDGCHC 1 cut(s) 711
SfaNI GCATC 1 cut(s) 737
SfuI TTCGAA 1 cut(s) 1003
SmlI CTYRAG 1 cut(s) 916
SmoI CTYRAG 1 cut(s) 916
Sse9I AATT 5 cut(s) 23, 207, 499, 520, 900
SsiI CCGC 4 cut(s) 261, 414, 792, 966
SspI AATATT 1 cut(s) 628
SspMI CTAG 2 cut(s) 671, 1024
StyD4I CCNGG 4 cut(s) 383, 720, 775, 989
StyI CCWWGG 1 cut(s) 768
TaaI ACNGT 2 cut(s) 609, 938
TaiI ACGT 2 cut(s) 538, 573
TaqI TCGA 4 cut(s) 284, 734, 742, 1003
TasI AATT 5 cut(s) 23, 207, 499, 520, 900
TatI WGTACW 2 cut(s) 232, 492
TfiI GAWTC 3 cut(s) 257, 739, 891
Tru1I TTAA 4 cut(s) 348, 515, 801, 917
Tru9I TTAA 4 cut(s) 348, 515, 801, 917
TseFI GTSAC 2 cut(s) 416, 566
TseI GCWGC 4 cut(s) 725, 883, 971, 980
Tsp45I GTSAC 2 cut(s) 416, 566
TspDTI ATGAA 2 cut(s) 200, 478
Van91I CCANNNNNTGG 1 cut(s) 990
Vha464I CTTAAG 1 cut(s) 916
VneI GTGCAC 1 cut(s) 707
XapI RAATTY 2 cut(s) 207, 499
XspI CTAG 2 cut(s) 671, 1024
ZraI GACGTC 1 cut(s) 536
ZrmI AGTACT 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.