Prupe.5G207200_v2.0.a1

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001329
Physical Location & Seq
Reverse (-)
16535158 .. 16538509
3352 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G207200.1

Sequence Viewer

Length: 1032 bp
ATGCAGAGGCTCCTTTCTTTGAAACTAGCCTCAAACCTCCCCAAAAATCTCCCAAACTCCTCCACTACCTCTTCACTCTTCCACTTCCAACCTCACAAAACGCTTTGTCTCACCAAACCACCCCAAACCCAAACTCAAAGCTACTTCTTTAGCTCCTTTCCTGCACACCCGATTCGCCATCTTCCTCATTCATGGAGGTTTCAGCACACTCTCACACAAAAAATCCATGGGGTTCTCTCCAACCCATTACTCAAACGGCACTTTTTTTTTGGTTTCCCAAATACCCTTATGAGAGTCTCAAGCAAGAGTCTTTCATCAGAGTTCAAACCTGCTGGTTTTTTCAGAGCCCAAATTCGCCAACACATTTTTAAGTTCAACCCAAATGAGAGTTCCCCCTGGAGATCATGGTTCCGAAGGGTATCAGAGGGTGAGGTGGTTTTGGGATTGATTATAGCTAATGTTGCCATGTTTATGTTGTGGAGGATTGCAGATAATAGCTTCATGGTGAAGAATTTTACCATTTCATTAGACAATTTTACAAGTGGACGTTTGCACACATTGATAACTTGTGCATTCAGTCATATCGATGCTGGGCATGTCACTTCGAACATGCTTGGACTATATGTTTTTGGGAAGCGTATTGAAAGAATTTTTGGGCCCGAGTTTTTTTTTAAGCTGTATCTAGCTGGGGCTGTTGGTGGCTCGGTCTTCTTCTTGGTGCACAAAGCCTACCTGGCTGCGTCATCGAAGGGCGGACAACCGATGAAGATGGACCCATCAAAGATACAGGGACTGGGCTCAAGTGGGGCGGTTACCGCGATCATGTTGCTTGATATATTCCTCTTCCCAAAATCTACCATCTACTTGGAGCTTATCATACCAGTTCCTGCCATGCTGGTGGGGATCTGTATAATTGCGAAAGATCTCATGAGGATAATACGGGGAGACCAACGTATCTCAGGATCTGCACACTTGGGTGGTGCTGCAGTTGCAGCCATAGCCTGGGCTCGAATTTGGAGGGGACGTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

344

Amino Acids

38.52

Weight (kDa)

10.69

Isoelectric Point (pI)

31.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 337
AccB7I CCANNNNNTGG 1 cut(s) 1002
AccII CGCG 1 cut(s) 818
AciI CCGC 3 cut(s) 753, 809, 816
AclWI GGATC 2 cut(s) 911, 970
AcsI RAATTY 4 cut(s) 351, 511, 648, 1011
AfiI CCNNNNNNNGG 1 cut(s) 1002
AgsI TTSAA 4 cut(s) 22, 325, 376, 644
AjnI CCWGG 3 cut(s) 395, 732, 1001
AjuI GAANNNNNNNTTGG 4 cut(s) 373, 405, 636, 668
AleI CACNNNNGTG 1 cut(s) 975
AluBI AGCT 7 cut(s) 141, 153, 455, 498, 676, 686, 871
AluI AGCT 7 cut(s) 141, 153, 455, 498, 676, 686, 871
Alw21I GWGCWC 1 cut(s) 723
Alw26I GTCTC 3 cut(s) 113, 301, 939
Alw44I GTGCAC 1 cut(s) 719
AlwI GGATC 2 cut(s) 911, 970
AlwNI CAGNNNCTG 3 cut(s) 793, 887, 965
Ama87I CYCGRG 1 cut(s) 659
AoxI GGCC 1 cut(s) 656
ApaI GGGCCC 1 cut(s) 660
ApaLI GTGCAC 1 cut(s) 719
ApeKI GCWGC 3 cut(s) 737, 983, 992
ApoI RAATTY 4 cut(s) 351, 511, 648, 1011
AspS9I GGNCC 3 cut(s) 656, 657, 772
AsuHPI GGTGA 3 cut(s) 103, 440, 517
AsuII TTCGAA 1 cut(s) 605
AvaI CYCGRG 1 cut(s) 659
AvaII GGWCC 1 cut(s) 772
BaeGI GKGCMC 2 cut(s) 660, 723
BaeI ACNNNNGTAYC 2 cut(s) 937, 970
BanII GRGCYC 4 cut(s) 349, 660, 800, 1009
BbsI GAAGAC 1 cut(s) 700
Bbv12I GWGCWC 1 cut(s) 723
BbvI GCAGC 3 cut(s) 724, 970, 1004
BccI CCATC 4 cut(s) 186, 763, 784, 866
BceAI ACGGC 1 cut(s) 272
BcgI CGANNNNNNTGC 2 cut(s) 808, 842
BciT130I CCWGG 3 cut(s) 397, 734, 1003
BcoDI GTCTC 3 cut(s) 113, 301, 939
BfaI CTAG 2 cut(s) 26, 683
BfmI CTRYAG 1 cut(s) 984
BfuAI ACCTGC 1 cut(s) 337
BglI GCCNNNNNGGC 1 cut(s) 734
BglII AGATCT 1 cut(s) 922
BisI GCNGC 3 cut(s) 738, 984, 993
BlsI GCNGC 3 cut(s) 739, 985, 994
Bme1390I CCNGG 3 cut(s) 397, 734, 1003
Bme18I GGWCC 1 cut(s) 772
BmeT110I CYCGRG 1 cut(s) 659
BmgT120I GGNCC 3 cut(s) 656, 657, 772
BmiI GGNNCC 4 cut(s) 11, 410, 658, 774
BmrFI CCNGG 3 cut(s) 397, 734, 1003
BmrI ACTGGG 1 cut(s) 803
BmsI GCATC 1 cut(s) 577
BmuI ACTGGG 1 cut(s) 803
BpiI GAAGAC 1 cut(s) 700
BpmI CTGGAG 1 cut(s) 418
Bpu14I TTCGAA 1 cut(s) 605
BpuEI CTTGAG 2 cut(s) 283, 784
Bsa29I ATCGAT 1 cut(s) 585
BsaI GGTCTC 1 cut(s) 939
BsaJI CCNNGG 3 cut(s) 226, 395, 1002
BsaXI ACNNNNNCTCC 1 cut(s) 1009
Bsc4I CCNNNNNNNGG 1 cut(s) 1002
Bse1I ACTGG 2 cut(s) 798, 881
BseBI CCWGG 3 cut(s) 397, 734, 1003
BseCI ATCGAT 1 cut(s) 585
BseDI CCNNGG 3 cut(s) 226, 395, 1002
BseLI CCNNNNNNNGG 1 cut(s) 1002
BseMII CTCAG 1 cut(s) 972
BseNI ACTGG 2 cut(s) 798, 881
BseRI GAGGAG 1 cut(s) 49
BseSI GKGCMC 2 cut(s) 660, 723
BseXI GCAGC 3 cut(s) 724, 970, 1004
BseYI CCCAGC 2 cut(s) 590, 686
BsgI GTGCAG 2 cut(s) 147, 951
Bsh1236I CGCG 1 cut(s) 818
BshFI GGCC 1 cut(s) 658
BshVI ATCGAT 1 cut(s) 585
BsiHKAI GWGCWC 1 cut(s) 723
BsiHKCI CYCGRG 1 cut(s) 659
BslFI GGGAC 1 cut(s) 804
BslI CCNNNNNNNGG 1 cut(s) 1002
BsmAI GTCTC 3 cut(s) 113, 301, 939
BsmFI GGGAC 1 cut(s) 804
BsmI GAATGC 1 cut(s) 572
BsnI GGCC 1 cut(s) 658
Bso31I GGTCTC 1 cut(s) 939
BsoBI CYCGRG 1 cut(s) 659
Bsp119I TTCGAA 1 cut(s) 605
Bsp120I GGGCCC 1 cut(s) 656
Bsp1286I GDGCHC 5 cut(s) 349, 660, 723, 800, 1009
Bsp143I GATC 5 cut(s) 401, 819, 903, 922, 962
Bsp19I CCATGG 1 cut(s) 226
BspACI CCGC 3 cut(s) 753, 809, 816
BspANI GGCC 1 cut(s) 658
BspCNI CTCAG 1 cut(s) 971
BspDI ATCGAT 1 cut(s) 585
BspFNI CGCG 1 cut(s) 818
BspHI TCATGA 1 cut(s) 927
BspLI GGNNCC 4 cut(s) 11, 410, 658, 774
BspMAI CTGCAG 1 cut(s) 988
BspMI ACCTGC 1 cut(s) 337
BspPI GGATC 2 cut(s) 911, 970
BspT104I TTCGAA 1 cut(s) 605
BspTNI GGTCTC 1 cut(s) 939
BsrI ACTGG 2 cut(s) 798, 881
BssECI CCNNGG 3 cut(s) 226, 395, 1002
BssMI GATC 5 cut(s) 401, 819, 903, 922, 962
BssT1I CCWWGG 1 cut(s) 226
Bst2UI CCWGG 3 cut(s) 397, 734, 1003
Bst6I CTCTTC 3 cut(s) 76, 83, 848
BstBI TTCGAA 1 cut(s) 605
BstDEI CTNAG 1 cut(s) 958
BstDSI CCRYGG 1 cut(s) 226
BstEII GGTNACC 1 cut(s) 811
BstFNI CGCG 1 cut(s) 818
BstKTI GATC 5 cut(s) 404, 822, 906, 925, 965
BstMAI GTCTC 3 cut(s) 113, 301, 939
BstMBI GATC 5 cut(s) 401, 819, 903, 922, 962
BstMWI GCNNNNNNNGC 6 cut(s) 461, 734, 815, 989, 992, 998
BstNI CCWGG 3 cut(s) 397, 734, 1003
BstNSI RCATGY 2 cut(s) 599, 613
BstPI GGTNACC 1 cut(s) 811
BstSCI CCNGG 3 cut(s) 395, 732, 1001
BstSFI CTRYAG 1 cut(s) 984
BstSLI GKGCMC 2 cut(s) 660, 723
BstUI CGCG 1 cut(s) 818
BstV1I GCAGC 3 cut(s) 724, 970, 1004
BstV2I GAAGAC 1 cut(s) 700
BstX2I RGATCY 3 cut(s) 903, 922, 962
BstXI CCANNNNNNTGG 2 cut(s) 865, 898
BstYI RGATCY 3 cut(s) 903, 922, 962
Bsu15I ATCGAT 1 cut(s) 585
BsuRI GGCC 1 cut(s) 658
BsuTUI ATCGAT 1 cut(s) 585
BtgI CCRYGG 1 cut(s) 226
BveI ACCTGC 1 cut(s) 337
CaiI CAGNNNCTG 3 cut(s) 793, 887, 965
CciI TCATGA 1 cut(s) 927
Cfr13I GGNCC 3 cut(s) 656, 657, 772
ClaI ATCGAT 1 cut(s) 585
CseI GACGC 1 cut(s) 729
DdeI CTNAG 1 cut(s) 958
DpnI GATC 5 cut(s) 403, 821, 905, 924, 964
DpnII GATC 5 cut(s) 401, 819, 903, 922, 962
Eam1104I CTCTTC 3 cut(s) 76, 83, 848
EarI CTCTTC 3 cut(s) 76, 83, 848
EciI GGCGGA 1 cut(s) 768
Eco130I CCWWGG 1 cut(s) 226
Eco24I GRGCYC 4 cut(s) 349, 660, 800, 1009
Eco31I GGTCTC 1 cut(s) 939
Eco47I GGWCC 1 cut(s) 772
Eco88I CYCGRG 1 cut(s) 659
Eco91I GGTNACC 1 cut(s) 811
EcoO65I GGTNACC 1 cut(s) 811
EcoRII CCWGG 3 cut(s) 395, 732, 1001
EcoT14I CCWWGG 1 cut(s) 226
EcoT38I GRGCYC 4 cut(s) 349, 660, 800, 1009
ErhI CCWWGG 1 cut(s) 226
FaqI GGGAC 1 cut(s) 804
Fnu4HI GCNGC 3 cut(s) 738, 984, 993
FriOI GRGCYC 4 cut(s) 349, 660, 800, 1009
Fsp4HI GCNGC 3 cut(s) 738, 984, 993
FspBI CTAG 2 cut(s) 26, 683
GluI GCNGC 3 cut(s) 738, 984, 993
GsaI CCCAGC 2 cut(s) 594, 690
GsuI CTGGAG 1 cut(s) 418
HaeIII GGCC 1 cut(s) 658
HgaI GACGC 1 cut(s) 729
HinfI GANTC 3 cut(s) 172, 294, 307
HphI GGTGA 3 cut(s) 103, 440, 517
Hpy166II GTNNAC 2 cut(s) 545, 721
Hpy188I TCNGA 5 cut(s) 319, 344, 413, 424, 1031
Hpy188III TCNNGA 2 cut(s) 928, 960
Hpy8I GTNNAC 2 cut(s) 545, 721
HpyAV CCTTC 2 cut(s) 408, 742
HpyCH4IV ACGT 3 cut(s) 547, 952, 1024
HpyCH4V TGCA 9 cut(s) 4, 164, 488, 553, 572, 721, 968, 986, 992
HpyF10VI GCNNNNNNNGC 6 cut(s) 461, 734, 815, 989, 992, 998
HpyF3I CTNAG 1 cut(s) 958
HpySE526I ACGT 3 cut(s) 547, 952, 1024
Kzo9I GATC 5 cut(s) 401, 819, 903, 922, 962
LmnI GCTCC 3 cut(s) 15, 158, 868
Lsp1109I GCAGC 3 cut(s) 724, 970, 1004
LweI GCATC 1 cut(s) 577
MaeI CTAG 2 cut(s) 26, 683
MaeII ACGT 3 cut(s) 547, 952, 1024
MaeIII GTNAC 2 cut(s) 598, 811
MalI GATC 5 cut(s) 403, 821, 905, 924, 964
MboI GATC 5 cut(s) 401, 819, 903, 922, 962
MboII GAAGA 8 cut(s) 63, 70, 173, 520, 700, 703, 778, 835
MflI RGATCY 3 cut(s) 903, 922, 962
MhlI GDGCHC 5 cut(s) 349, 660, 723, 800, 1009
MluCI AATT 6 cut(s) 351, 511, 532, 648, 912, 1011
MlyI GAGTC 2 cut(s) 303, 316
MmeI TCCRAC 2 cut(s) 112, 264
MseI TTAA 2 cut(s) 369, 672
MslI CAYNNNNRTG 4 cut(s) 470, 585, 896, 975
MspR9I CCNGG 3 cut(s) 397, 734, 1003
Mva1269I GAATGC 1 cut(s) 572
MvaI CCWGG 3 cut(s) 397, 734, 1003
MvnI CGCG 1 cut(s) 818
MwoI GCNNNNNNNGC 6 cut(s) 461, 734, 815, 989, 992, 998
NcoI CCATGG 1 cut(s) 226
NdeII GATC 5 cut(s) 401, 819, 903, 922, 962
NlaIV GGNNCC 4 cut(s) 11, 410, 658, 774
NmuCI GTSAC 1 cut(s) 598
NspI RCATGY 2 cut(s) 599, 613
NspV TTCGAA 1 cut(s) 605
OliI CACNNNNGTG 1 cut(s) 975
PagI TCATGA 1 cut(s) 927
PctI GAATGC 1 cut(s) 572
PfeI GAWTC 1 cut(s) 172
PflMI CCANNNNNTGG 1 cut(s) 1002
PkrI GCNGC 3 cut(s) 739, 985, 994
PleI GAGTC 2 cut(s) 302, 315
PpsI GAGTC 2 cut(s) 302, 315
Psp6I CCWGG 3 cut(s) 395, 732, 1001
PspEI GGTNACC 1 cut(s) 811
PspFI CCCAGC 2 cut(s) 590, 686
PspGI CCWGG 3 cut(s) 395, 732, 1001
PspN4I GGNNCC 4 cut(s) 11, 410, 658, 774
PspOMI GGGCCC 1 cut(s) 656
PspPI GGNCC 3 cut(s) 656, 657, 772
PstI CTGCAG 1 cut(s) 988
PstNI CAGNNNCTG 3 cut(s) 793, 887, 965
PsuI RGATCY 3 cut(s) 903, 922, 962
RseI CAYNNNNRTG 4 cut(s) 470, 585, 896, 975
SaqAI TTAA 2 cut(s) 369, 672
SatI GCNGC 3 cut(s) 738, 984, 993
Sau3AI GATC 5 cut(s) 401, 819, 903, 922, 962
Sau96I GGNCC 3 cut(s) 656, 657, 772
SchI GAGTC 2 cut(s) 303, 316
ScrFI CCNGG 3 cut(s) 397, 734, 1003
SduI GDGCHC 5 cut(s) 349, 660, 723, 800, 1009
SfaNI GCATC 1 cut(s) 577
SfcI CTRYAG 1 cut(s) 984
SfuI TTCGAA 1 cut(s) 605
SinI GGWCC 1 cut(s) 772
SmiMI CAYNNNNRTG 4 cut(s) 470, 585, 896, 975
SmlI CTYRAG 2 cut(s) 298, 799
SmoI CTYRAG 2 cut(s) 298, 799
Sse9I AATT 6 cut(s) 351, 511, 532, 648, 912, 1011
SsiI CCGC 3 cut(s) 753, 809, 816
SspMI CTAG 2 cut(s) 26, 683
StyD4I CCNGG 3 cut(s) 395, 732, 1001
StyI CCWWGG 1 cut(s) 226
TaiI ACGT 3 cut(s) 550, 955, 1027
TaqI TCGA 4 cut(s) 585, 605, 746, 1009
TaqII GACCGA 1 cut(s) 694
TasI AATT 6 cut(s) 351, 511, 532, 648, 912, 1011
TfiI GAWTC 1 cut(s) 172
Tru1I TTAA 2 cut(s) 369, 672
Tru9I TTAA 2 cut(s) 369, 672
TseFI GTSAC 1 cut(s) 598
TseI GCWGC 3 cut(s) 737, 983, 992
Tsp45I GTSAC 1 cut(s) 598
TspDTI ATGAA 5 cut(s) 180, 303, 490, 513, 779
Van91I CCANNNNNTGG 1 cut(s) 1002
VneI GTGCAC 1 cut(s) 719
VpaK11BI GGWCC 1 cut(s) 772
XapI RAATTY 4 cut(s) 351, 511, 648, 1011
XceI RCATGY 2 cut(s) 599, 613
XspI CTAG 2 cut(s) 26, 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.