Prupe.3G124800_v2.0.a1

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001329
Physical Location & Seq
Forward (+)
11423928 .. 11427578
3651 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G124800.2

Sequence Viewer

Length: 1047 bp
ATGCAGAGGCTCGCTTCTTTGAAACTGGCCTCAAACTTCCCCAAAAAGCTTTCAAACTCCTCCACCACCACTACTACTTCTTCAATCTTCCACTTTCAACCACACAAATCGGTTTCTCTCACCAAACCACCCCAAACCCAAACCCAAAACTACTTCTTTAGCTCCTTCCCCGCACACCGGCTTCACCGTCTTCCTCATTCATGGAGTTTGCAGCACACTCTGACACAAAAGATTCATGGGTTGCTCTCCAACACATTACTCAGACGGCAGTTTTTGGTGGGTTTCTCAAATACCCTTTTGAGAGCCTCAAGCAAGAGTCTTTCAGATTTCAGGCTTGGCTTTCTCAGAGTCCAATTTTCGAGACAGAGTGCTAAGTTCAAGTTCAACCCAAAGTTTACTTCTCCTCGGAGTGTCGGGAGATCATGGCTCCGAAGGGTATCAGAGGGTGAGGACAATGACGTGGTTTTGGGATTGATTATAGCTAATGCTGCAGTGTTTTTGTTATGGAAGATTGCCGATACTGACTTCATGGTGAAGAATTTTATTAATGCATTAATCAATGTTAGAAGTGGACGTCTTCACACGTTGATAACTTCTGCCTTCAGTCATATCGGCACTGAGCATTTCATTCATAACATGATTGGACTATATGTTTTTGGGGCGCATATTGAAAGAATTTTTGGGCCTCAATTTTTGCTGGGATTGTATCTAGCTGGGGCTCTTGGTGGCTCGGTCTTTTTCTTGGTGCACAAAACCTACCTGGCTGTGTCATCGATGGGCCGGCGAGAGGAGAACAAAGACCCAAACAACCTGGCACTGGGCGCAGGCGGGGCCGTTCATGCTATCATTTTGCTTGATATATTCCTCTTCCCAAAATCCATCTCTACTCCGGGATTCTCAAAACCGTTGTTCCTGGGGATCTTTCTAATTGGGAAAGATATCTTGAGGTTAATACTGGGAGACGAACAAATCTCAGGGGCTACACAATTGGGAGGTGCTGCAGTTGCAGCCATTGCCTGGACACGACTTCGAAAGGGGCGATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

349

Amino Acids

38.71

Weight (kDa)

11.12

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 577
AccB7I CCANNNNNTGG 1 cut(s) 1017
AciI CCGC 2 cut(s) 171, 828
AclWI GGATC 1 cut(s) 926
AcsI RAATTY 2 cut(s) 538, 675
AcuI CTGAAG 1 cut(s) 586
AcyI GRCGYC 1 cut(s) 574
AfiI CCNNNNNNNGG 4 cut(s) 177, 787, 817, 1017
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 7 cut(s) 22, 54, 84, 98, 379, 385, 671
AjiI CACGTC 1 cut(s) 460
AjnI CCWGG 4 cut(s) 759, 810, 912, 1016
AjuI GAANNNNNNNTTGG 4 cut(s) 382, 414, 663, 695
AluBI AGCT 4 cut(s) 49, 162, 482, 713
AluI AGCT 4 cut(s) 49, 162, 482, 713
Alw21I GWGCWC 1 cut(s) 750
Alw26I GTCTC 2 cut(s) 355, 954
Alw44I GTGCAC 1 cut(s) 746
AlwI GGATC 1 cut(s) 926
AoxI GGCC 4 cut(s) 27, 683, 778, 831
ApaLI GTGCAC 1 cut(s) 746
ApeKI GCWGC 4 cut(s) 211, 488, 998, 1007
ApoI RAATTY 2 cut(s) 538, 675
AseI ATTAAT 2 cut(s) 546, 554
AspLEI GCGC 2 cut(s) 664, 824
AspS9I GGNCC 3 cut(s) 683, 778, 831
AsuC2I CCSGG 1 cut(s) 891
AsuHPI GGTGA 4 cut(s) 112, 176, 458, 544
AsuII TTCGAA 1 cut(s) 1030
BaeGI GKGCMC 1 cut(s) 750
BanII GRGCYC 1 cut(s) 721
BbsI GAAGAC 2 cut(s) 182, 569
Bbv12I GWGCWC 1 cut(s) 750
BbvI GCAGC 4 cut(s) 223, 475, 985, 1019
BccI CCATC 2 cut(s) 769, 887
BceAI ACGGC 2 cut(s) 281, 818
BciT130I CCWGG 4 cut(s) 761, 812, 914, 1018
BcnI CCSGG 1 cut(s) 891
BcoDI GTCTC 2 cut(s) 355, 954
BfaI CTAG 1 cut(s) 710
BfmI CTRYAG 2 cut(s) 489, 999
BisI GCNGC 4 cut(s) 212, 489, 999, 1008
BlsI GCNGC 4 cut(s) 213, 490, 1000, 1009
Bme1390I CCNGG 5 cut(s) 761, 812, 891, 914, 1018
BmgBI CACGTC 1 cut(s) 460
BmgT120I GGNCC 3 cut(s) 683, 778, 831
BmiI GGNNCC 2 cut(s) 428, 832
BmrFI CCNGG 5 cut(s) 761, 812, 891, 914, 1018
BmrI ACTGGG 2 cut(s) 827, 965
BmuI ACTGGG 2 cut(s) 827, 965
BpiI GAAGAC 2 cut(s) 182, 569
Bpu14I TTCGAA 1 cut(s) 1030
BpuEI CTTGAG 2 cut(s) 292, 964
BpuMI CCSGG 1 cut(s) 891
Bsa29I ATCGAT 1 cut(s) 773
BsaHI GRCGYC 1 cut(s) 574
BsaJI CCNNGG 2 cut(s) 404, 913
Bsc4I CCNNNNNNNGG 4 cut(s) 177, 787, 817, 1017
Bse118I RCCGGY 2 cut(s) 177, 780
Bse1I ACTGG 3 cut(s) 30, 822, 960
Bse3DI GCAATG 1 cut(s) 1011
BseBI CCWGG 4 cut(s) 761, 812, 914, 1018
BseCI ATCGAT 1 cut(s) 773
BseDI CCNNGG 2 cut(s) 404, 913
BseLI CCNNNNNNNGG 4 cut(s) 177, 787, 817, 1017
BseMI GCAATG 1 cut(s) 1011
BseMII CTCAG 4 cut(s) 274, 358, 609, 987
BseNI ACTGG 3 cut(s) 30, 822, 960
BseRI GAGGAG 3 cut(s) 49, 393, 803
BseSI GKGCMC 1 cut(s) 750
BseXI GCAGC 4 cut(s) 223, 475, 985, 1019
BseYI CCCAGC 2 cut(s) 697, 713
BshFI GGCC 4 cut(s) 29, 685, 780, 833
BshVI ATCGAT 1 cut(s) 773
BsiHKAI GWGCWC 1 cut(s) 750
BsiSI CCGG 3 cut(s) 178, 781, 890
BslI CCNNNNNNNGG 4 cut(s) 177, 787, 817, 1017
BsmAI GTCTC 2 cut(s) 355, 954
BsmBI CGTCTC 1 cut(s) 954
BsnI GGCC 4 cut(s) 29, 685, 780, 833
Bsp119I TTCGAA 1 cut(s) 1030
Bsp1286I GDGCHC 2 cut(s) 721, 750
Bsp143I GATC 2 cut(s) 419, 918
BspACI CCGC 2 cut(s) 171, 828
BspANI GGCC 4 cut(s) 29, 685, 780, 833
BspCNI CTCAG 4 cut(s) 273, 357, 610, 986
BspDI ATCGAT 1 cut(s) 773
BspLI GGNNCC 2 cut(s) 428, 832
BspMAI CTGCAG 2 cut(s) 493, 1003
BspPI GGATC 1 cut(s) 926
BspT104I TTCGAA 1 cut(s) 1030
BsrDI GCAATG 1 cut(s) 1011
BsrFI RCCGGY 2 cut(s) 177, 780
BsrI ACTGG 3 cut(s) 30, 822, 960
BssAI RCCGGY 2 cut(s) 177, 780
BssECI CCNNGG 2 cut(s) 404, 913
BssMI GATC 2 cut(s) 419, 918
BssNI GRCGYC 1 cut(s) 574
Bst2UI CCWGG 4 cut(s) 761, 812, 914, 1018
Bst4CI ACNGT 2 cut(s) 188, 906
Bst6I CTCTTC 1 cut(s) 872
BstACI GRCGYC 1 cut(s) 574
BstAPI GCANNNNNTGC 1 cut(s) 1013
BstBI TTCGAA 1 cut(s) 1030
BstC8I GCNNGC 3 cut(s) 12, 782, 826
BstDEI CTNAG 5 cut(s) 260, 344, 372, 618, 973
BstHHI GCGC 2 cut(s) 664, 824
BstKTI GATC 2 cut(s) 422, 921
BstMAI GTCTC 2 cut(s) 355, 954
BstMBI GATC 2 cut(s) 419, 918
BstMWI GCNNNNNNNGC 7 cut(s) 488, 821, 830, 839, 1004, 1007, 1013
BstNI CCWGG 4 cut(s) 761, 812, 914, 1018
BstSCI CCNGG 5 cut(s) 759, 810, 889, 912, 1016
BstSFI CTRYAG 2 cut(s) 489, 999
BstSLI GKGCMC 1 cut(s) 750
BstV1I GCAGC 4 cut(s) 223, 475, 985, 1019
BstV2I GAAGAC 2 cut(s) 182, 569
BstX2I RGATCY 1 cut(s) 918
BstYI RGATCY 1 cut(s) 918
Bsu15I ATCGAT 1 cut(s) 773
BsuRI GGCC 4 cut(s) 29, 685, 780, 833
BsuTUI ATCGAT 1 cut(s) 773
BtrI CACGTC 1 cut(s) 460
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 3 cut(s) 498, 615, 815
Cac8I GCNNGC 3 cut(s) 12, 782, 826
CfoI GCGC 2 cut(s) 664, 824
Cfr10I RCCGGY 2 cut(s) 177, 780
Cfr13I GGNCC 3 cut(s) 683, 778, 831
ClaI ATCGAT 1 cut(s) 773
CviAII CATG 6 cut(s) 201, 236, 423, 529, 637, 839
DdeI CTNAG 5 cut(s) 260, 344, 372, 618, 973
DpnI GATC 2 cut(s) 421, 920
DpnII GATC 2 cut(s) 419, 918
Eam1104I CTCTTC 1 cut(s) 872
EarI CTCTTC 1 cut(s) 872
Eco24I GRGCYC 1 cut(s) 721
Eco32I GATATC 1 cut(s) 940
Eco57I CTGAAG 1 cut(s) 586
EcoRII CCWGG 4 cut(s) 759, 810, 912, 1016
EcoRV GATATC 1 cut(s) 940
EcoT22I ATGCAT 1 cut(s) 553
EcoT38I GRGCYC 1 cut(s) 721
Esp3I CGTCTC 1 cut(s) 954
FaeI CATG 6 cut(s) 204, 239, 426, 532, 640, 842
FatI CATG 6 cut(s) 200, 235, 422, 528, 636, 838
FauI CCCGC 2 cut(s) 178, 821
Fnu4HI GCNGC 4 cut(s) 212, 489, 999, 1008
FriOI GRGCYC 1 cut(s) 721
Fsp4HI GCNGC 4 cut(s) 212, 489, 999, 1008
FspBI CTAG 1 cut(s) 710
GlaI GCGC 2 cut(s) 663, 823
GluI GCNGC 4 cut(s) 212, 489, 999, 1008
GsaI CCCAGC 2 cut(s) 701, 717
HaeIII GGCC 4 cut(s) 29, 685, 780, 833
HapII CCGG 3 cut(s) 178, 781, 890
HhaI GCGC 2 cut(s) 664, 824
Hin1I GRCGYC 1 cut(s) 574
Hin1II CATG 6 cut(s) 204, 239, 426, 532, 640, 842
Hin6I GCGC 2 cut(s) 662, 822
HinP1I GCGC 2 cut(s) 662, 822
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 5 cut(s) 232, 316, 348, 894, 1041
HpaII CCGG 3 cut(s) 178, 781, 890
HphI GGTGA 4 cut(s) 112, 176, 458, 544
Hpy166II GTNNAC 3 cut(s) 396, 572, 748
Hpy188I TCNGA 7 cut(s) 222, 263, 325, 347, 408, 431, 442
Hpy188III TCNNGA 3 cut(s) 360, 415, 943
Hpy8I GTNNAC 3 cut(s) 396, 572, 748
HpyAV CCTTC 3 cut(s) 175, 426, 610
HpyCH4III ACNGT 2 cut(s) 188, 906
HpyCH4IV ACGT 3 cut(s) 459, 574, 584
HpyCH4V TGCA 7 cut(s) 4, 211, 491, 551, 748, 1001, 1007
HpyF10VI GCNNNNNNNGC 7 cut(s) 488, 821, 830, 839, 1004, 1007, 1013
HpyF3I CTNAG 5 cut(s) 260, 344, 372, 618, 973
HpySE526I ACGT 3 cut(s) 459, 574, 584
Hsp92I GRCGYC 1 cut(s) 574
Hsp92II CATG 6 cut(s) 204, 239, 426, 532, 640, 842
HspAI GCGC 2 cut(s) 662, 822
KroI GCCGGC 1 cut(s) 780
KroNI GCCGGC 1 cut(s) 782
Kzo9I GATC 2 cut(s) 419, 918
LmnI GCTCC 2 cut(s) 167, 432
Lsp1109I GCAGC 4 cut(s) 223, 475, 985, 1019
MaeI CTAG 1 cut(s) 710
MaeII ACGT 3 cut(s) 459, 574, 584
MalI GATC 2 cut(s) 421, 920
MboI GATC 2 cut(s) 419, 918
MboII GAAGA 7 cut(s) 72, 79, 182, 520, 547, 569, 859
MfeI CAATTG 1 cut(s) 986
MflI RGATCY 1 cut(s) 918
MhlI GDGCHC 2 cut(s) 721, 750
MluCI AATT 6 cut(s) 353, 538, 675, 689, 927, 986
MlyI GAGTC 2 cut(s) 325, 357
MmeI TCCRAC 1 cut(s) 273
Mph1103I ATGCAT 1 cut(s) 553
MroNI GCCGGC 1 cut(s) 780
MseI TTAA 3 cut(s) 546, 554, 950
MspI CCGG 3 cut(s) 178, 781, 890
MspR9I CCNGG 5 cut(s) 761, 812, 891, 914, 1018
MunI CAATTG 1 cut(s) 986
MvaI CCWGG 4 cut(s) 761, 812, 914, 1018
MwoI GCNNNNNNNGC 7 cut(s) 488, 821, 830, 839, 1004, 1007, 1013
NaeI GCCGGC 1 cut(s) 782
NciI CCSGG 1 cut(s) 891
NdeII GATC 2 cut(s) 419, 918
NgoMIV GCCGGC 1 cut(s) 780
NlaIII CATG 6 cut(s) 204, 239, 426, 532, 640, 842
NlaIV GGNNCC 2 cut(s) 428, 832
NsiI ATGCAT 1 cut(s) 553
NspV TTCGAA 1 cut(s) 1030
PcsI WCGNNNNNNNCGW 1 cut(s) 1036
PdiI GCCGGC 1 cut(s) 782
PfeI GAWTC 3 cut(s) 232, 894, 1041
PflMI CCANNNNNTGG 1 cut(s) 1017
PfoI TCCNGGA 1 cut(s) 889
PkrI GCNGC 4 cut(s) 213, 490, 1000, 1009
PleI GAGTC 2 cut(s) 324, 356
PpsI GAGTC 2 cut(s) 324, 356
PshBI ATTAAT 2 cut(s) 546, 554
Psp6I CCWGG 4 cut(s) 759, 810, 912, 1016
PspFI CCCAGC 2 cut(s) 697, 713
PspGI CCWGG 4 cut(s) 759, 810, 912, 1016
PspN4I GGNNCC 2 cut(s) 428, 832
PspPI GGNCC 3 cut(s) 683, 778, 831
PstI CTGCAG 2 cut(s) 493, 1003
PsuI RGATCY 1 cut(s) 918
SaqAI TTAA 3 cut(s) 546, 554, 950
SatI GCNGC 4 cut(s) 212, 489, 999, 1008
Sau3AI GATC 2 cut(s) 419, 918
Sau96I GGNCC 3 cut(s) 683, 778, 831
SchI GAGTC 2 cut(s) 325, 357
ScrFI CCNGG 5 cut(s) 761, 812, 891, 914, 1018
SduI GDGCHC 2 cut(s) 721, 750
SfcI CTRYAG 2 cut(s) 489, 999
SfuI TTCGAA 1 cut(s) 1030
SmlI CTYRAG 2 cut(s) 307, 943
SmoI CTYRAG 2 cut(s) 307, 943
Sse9I AATT 6 cut(s) 353, 538, 675, 689, 927, 986
SsiI CCGC 2 cut(s) 171, 828
SspMI CTAG 1 cut(s) 710
StyD4I CCNGG 5 cut(s) 759, 810, 889, 912, 1016
TaaI ACNGT 2 cut(s) 188, 906
TaiI ACGT 3 cut(s) 462, 577, 587
TaqI TCGA 3 cut(s) 359, 773, 1030
TaqII GACCGA 1 cut(s) 721
TasI AATT 6 cut(s) 353, 538, 675, 689, 927, 986
TfiI GAWTC 3 cut(s) 232, 894, 1041
Tru1I TTAA 3 cut(s) 546, 554, 950
Tru9I TTAA 3 cut(s) 546, 554, 950
TscAI CASTG 3 cut(s) 498, 622, 822
TseI GCWGC 4 cut(s) 211, 488, 998, 1007
TspDTI ATGAA 6 cut(s) 189, 224, 517, 616, 620, 827
TspRI CASTG 3 cut(s) 498, 622, 822
Van91I CCANNNNNTGG 1 cut(s) 1017
VneI GTGCAC 1 cut(s) 746
VspI ATTAAT 2 cut(s) 546, 554
XapI RAATTY 2 cut(s) 538, 675
XspI CTAG 1 cut(s) 710
ZraI GACGTC 1 cut(s) 575
Zsp2I ATGCAT 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.