RLG00000005057

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001329
Physical Location & Seq
Forward (+)
64737512 .. 64741257
3746 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005057

Sequence Viewer

Length: 1032 bp
ATGCAGAGGCTTCTTTCTGTGAAATTAGCCTCAAACCTCCCCAAAAACCTATCAACCTCCGCCGCCACCACCACCCAAAGCTCTTCCCTTTTCCACTTCCAACCTCACAAAACCTTCTCTCTCACCAAACCACCCCAAACCCAGACCCAAAATCACTTCTTTAGCTCCTTCCCCACACACCCACTTCCCCATCAGCACAATCTCACTCACAAAATTCATGGCTTTCTCTCCAACCCAGTACTCAGAAAACACTTCTCTTTGGATTCCGCAAATACCCTTTTGAGGGTATCGAGCAAGAGGCTTTCGGATTGCAGGCTTGCCTTTCTCCAAAGCAAGTTTCCCAAACAGAGTTTTAACTTCAACCCAAGTCGTATTTCTAATCAGAGTGCCTGGAGAACATGGTTGCGAAGGTTATCAAGCGGTGACATGGTTTTGGGATTGATTGTAGCTAATGTTGCTGTTTTTCTTTTGTGGAGGATAGCAGACCCTATATTCATGTACAAGAATTTTACTATCTCATTAAACAATTTTACAAGTGGACGTCTTCACACATTGATAACTTCTGCATTTAGTCACGTTGATATTGGGCATATCATTTCTAACATGATTGGACTGTATTTTTTTGGGATCAATATTGGAAGAACTTTTGGGCCTGAGTTTTTGCTGAAGTTGTATCTAGCTGGAGCTATTGGTGGCTCAGTCTTTTACTTGGTGCACCATGCCTTCCTGGCTGCGTCATCGAAGAATCGACCATTTGGGATTATGGACGCTTCTAAGGCCCCAGGATTGGGGGCAAGCGGTGCTGTTAATGCTATCATGTTGCTTGATATATTCCTTCACCCCACAGCTACCCTCTACTTTGATTTTATCATACCAGTTCCTGCCATGCTGCTGGGAATCTTTCTAATTGGAAAGGATATCTTAAGAATAATGGAGGGAAACAGTCAGATCTCAGGATCTGCACACTTGGGCGGTGCGGCAGTAGCAGCCATAGCCTGGGCAAGACTTCGAAAGGGGCGTGGTTTATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

344

Amino Acids

37.88

Weight (kDa)

10.9

Isoelectric Point (pI)

35.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 180 - 333 9.5e-25 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 544
AccB7I CCANNNNNTGG 1 cut(s) 996
AciI CCGC 7 cut(s) 60, 63, 267, 420, 798, 972, 977
AclWI GGATC 2 cut(s) 635, 964
AcsI RAATTY 2 cut(s) 213, 505
AcuI CTGAAG 1 cut(s) 686
AcyI GRCGYC 1 cut(s) 541
AfaI GTAC 2 cut(s) 240, 500
AfiI CCNNNNNNNGG 5 cut(s) 282, 283, 787, 788, 996
AflII CTTAAG 1 cut(s) 922
AgsI TTSAA 1 cut(s) 361
AjnI CCWGG 4 cut(s) 389, 726, 781, 995
AjuI GAANNNNNNNTTGG 4 cut(s) 321, 353, 358, 390
AluBI AGCT 6 cut(s) 81, 165, 449, 680, 686, 848
AluI AGCT 6 cut(s) 81, 165, 449, 680, 686, 848
Alw21I GWGCWC 1 cut(s) 717
Alw44I GTGCAC 1 cut(s) 713
AlwI GGATC 2 cut(s) 635, 964
AlwNI CAGNNNCTG 2 cut(s) 881, 959
AoxI GGCC 2 cut(s) 650, 777
ApaLI GTGCAC 1 cut(s) 713
ApeKI GCWGC 3 cut(s) 731, 889, 986
ApoI RAATTY 2 cut(s) 213, 505
AspS9I GGNCC 2 cut(s) 650, 778
AsuHPI GGTGA 3 cut(s) 115, 434, 830
AsuII TTCGAA 1 cut(s) 1009
BaeGI GKGCMC 1 cut(s) 717
BbsI GAAGAC 1 cut(s) 536
Bbv12I GWGCWC 1 cut(s) 717
BbvI GCAGC 3 cut(s) 718, 876, 998
BccI CCATC 1 cut(s) 198
BciT130I CCWGG 4 cut(s) 391, 728, 783, 997
BfaI CTAG 2 cut(s) 677, 1030
BfrI CTTAAG 1 cut(s) 922
BglI GCCNNNNNGGC 1 cut(s) 728
BglII AGATCT 1 cut(s) 948
BisI GCNGC 5 cut(s) 63, 732, 890, 978, 987
BlsI GCNGC 5 cut(s) 64, 733, 891, 979, 988
BmcAI AGTACT 1 cut(s) 240
Bme1390I CCNGG 4 cut(s) 391, 728, 783, 997
BmgT120I GGNCC 2 cut(s) 650, 778
BmiI GGNNCC 1 cut(s) 780
BmrFI CCNGG 4 cut(s) 391, 728, 783, 997
BmrI ACTGGG 1 cut(s) 230
BmuI ACTGGG 1 cut(s) 230
BpiI GAAGAC 1 cut(s) 536
BpmI CTGGAG 2 cut(s) 412, 702
Bpu14I TTCGAA 1 cut(s) 1009
BsaHI GRCGYC 1 cut(s) 541
BsaJI CCNNGG 2 cut(s) 781, 996
Bsc4I CCNNNNNNNGG 5 cut(s) 282, 283, 787, 788, 996
Bse1I ACTGG 2 cut(s) 236, 875
BseBI CCWGG 4 cut(s) 391, 728, 783, 997
BseDI CCNNGG 2 cut(s) 781, 996
BseLI CCNNNNNNNGG 5 cut(s) 282, 283, 787, 788, 996
BseMII CTCAG 4 cut(s) 256, 645, 711, 966
BseNI ACTGG 2 cut(s) 236, 875
BseSI GKGCMC 1 cut(s) 717
BseXI GCAGC 3 cut(s) 718, 876, 998
BseYI CCCAGC 1 cut(s) 892
BsgI GTGCAG 1 cut(s) 945
BshFI GGCC 2 cut(s) 652, 779
BsiHKAI GWGCWC 1 cut(s) 717
BslI CCNNNNNNNGG 5 cut(s) 282, 283, 787, 788, 996
BsnI GGCC 2 cut(s) 652, 779
Bsp119I TTCGAA 1 cut(s) 1009
Bsp1286I GDGCHC 1 cut(s) 717
Bsp1407I TGTACA 1 cut(s) 498
Bsp143I GATC 3 cut(s) 627, 948, 956
BspACI CCGC 7 cut(s) 60, 63, 267, 420, 798, 972, 977
BspANI GGCC 2 cut(s) 652, 779
BspCNI CTCAG 4 cut(s) 255, 646, 710, 965
BspLI GGNNCC 1 cut(s) 780
BspPI GGATC 2 cut(s) 635, 964
BspQI GCTCTTC 1 cut(s) 88
BspT104I TTCGAA 1 cut(s) 1009
BspTI CTTAAG 1 cut(s) 922
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 2 cut(s) 236, 875
BssECI CCNNGG 2 cut(s) 781, 996
BssMI GATC 3 cut(s) 627, 948, 956
BssNI GRCGYC 1 cut(s) 541
Bst2UI CCWGG 4 cut(s) 391, 728, 783, 997
Bst4CI ACNGT 2 cut(s) 615, 944
Bst6I CTCTTC 1 cut(s) 88
BstACI GRCGYC 1 cut(s) 541
BstAFI CTTAAG 1 cut(s) 922
BstAPI GCANNNNNTGC 1 cut(s) 800
BstAUI TGTACA 1 cut(s) 498
BstBI TTCGAA 1 cut(s) 1009
BstC8I GCNNGC 3 cut(s) 314, 318, 796
BstDEI CTNAG 5 cut(s) 242, 654, 697, 774, 952
BstKTI GATC 3 cut(s) 630, 951, 959
BstMBI GATC 3 cut(s) 627, 948, 956
BstMWI GCNNNNNNNGC 8 cut(s) 455, 728, 776, 800, 809, 983, 986, 992
BstNI CCWGG 4 cut(s) 391, 728, 783, 997
BstSCI CCNGG 4 cut(s) 389, 726, 781, 995
BstSLI GKGCMC 1 cut(s) 717
BstV1I GCAGC 3 cut(s) 718, 876, 998
BstV2I GAAGAC 1 cut(s) 536
BstX2I RGATCY 2 cut(s) 948, 956
BstXI CCANNNNNNTGG 1 cut(s) 892
BstYI RGATCY 2 cut(s) 948, 956
BsuRI GGCC 2 cut(s) 652, 779
Cac8I GCNNGC 3 cut(s) 314, 318, 796
CaiI CAGNNNCTG 2 cut(s) 881, 959
Cfr13I GGNCC 2 cut(s) 650, 778
CseI GACGC 2 cut(s) 723, 776
Csp6I GTAC 2 cut(s) 239, 499
CviAII CATG 8 cut(s) 218, 399, 427, 496, 604, 719, 817, 886
CviQI GTAC 2 cut(s) 239, 499
DdeI CTNAG 5 cut(s) 242, 654, 697, 774, 952
DpnI GATC 3 cut(s) 629, 950, 958
DpnII GATC 3 cut(s) 627, 948, 956
Eam1104I CTCTTC 1 cut(s) 88
EarI CTCTTC 1 cut(s) 88
EciI GGCGGA 1 cut(s) 49
Eco32I GATATC 1 cut(s) 919
Eco57I CTGAAG 1 cut(s) 686
EcoO109I RGGNCCY 1 cut(s) 778
EcoRII CCWGG 4 cut(s) 389, 726, 781, 995
EcoRV GATATC 1 cut(s) 919
FaeI CATG 8 cut(s) 221, 402, 430, 499, 607, 722, 820, 889
FalI AAGNNNNNCTT 2 cut(s) 905, 937
FatI CATG 8 cut(s) 217, 398, 426, 495, 603, 718, 816, 885
Fnu4HI GCNGC 5 cut(s) 63, 732, 890, 978, 987
Fsp4HI GCNGC 5 cut(s) 63, 732, 890, 978, 987
FspBI CTAG 2 cut(s) 677, 1030
GluI GCNGC 5 cut(s) 63, 732, 890, 978, 987
GsaI CCCAGC 1 cut(s) 896
GsuI CTGGAG 2 cut(s) 412, 702
HaeIII GGCC 2 cut(s) 652, 779
HgaI GACGC 2 cut(s) 723, 776
Hin1I GRCGYC 1 cut(s) 541
Hin1II CATG 8 cut(s) 221, 402, 430, 499, 607, 722, 820, 889
HinfI GANTC 3 cut(s) 263, 745, 897
HphI GGTGA 3 cut(s) 115, 434, 830
Hpy166II GTNNAC 2 cut(s) 539, 715
Hpy188I TCNGA 4 cut(s) 245, 307, 384, 948
Hpy188III TCNNGA 1 cut(s) 954
Hpy8I GTNNAC 2 cut(s) 539, 715
HpyAV CCTTC 5 cut(s) 124, 178, 402, 733, 845
HpyCH4III ACNGT 2 cut(s) 615, 944
HpyCH4IV ACGT 2 cut(s) 541, 576
HpyCH4V TGCA 5 cut(s) 4, 312, 566, 715, 962
HpyF10VI GCNNNNNNNGC 8 cut(s) 455, 728, 776, 800, 809, 983, 986, 992
HpyF3I CTNAG 5 cut(s) 242, 654, 697, 774, 952
HpySE526I ACGT 2 cut(s) 541, 576
Hsp92I GRCGYC 1 cut(s) 541
Hsp92II CATG 8 cut(s) 221, 402, 430, 499, 607, 722, 820, 889
Kzo9I GATC 3 cut(s) 627, 948, 956
LguI GCTCTTC 1 cut(s) 88
LmnI GCTCC 2 cut(s) 170, 683
Lsp1109I GCAGC 3 cut(s) 718, 876, 998
MaeI CTAG 2 cut(s) 677, 1030
MaeII ACGT 2 cut(s) 541, 576
MaeIII GTNAC 2 cut(s) 422, 572
MalI GATC 3 cut(s) 629, 950, 958
MboI GATC 3 cut(s) 627, 948, 956
MboII GAAGA 4 cut(s) 75, 536, 651, 754
MflI RGATCY 2 cut(s) 948, 956
MhlI GDGCHC 1 cut(s) 717
MluCI AATT 5 cut(s) 23, 213, 505, 526, 906
MmeI TCCRAC 2 cut(s) 124, 255
MnlI CCTC 9 cut(s) 40, 47, 67, 114, 276, 291, 468, 863, 928
MseI TTAA 4 cut(s) 354, 521, 807, 923
MspCI CTTAAG 1 cut(s) 922
MspR9I CCNGG 4 cut(s) 391, 728, 783, 997
MvaI CCWGG 4 cut(s) 391, 728, 783, 997
MwoI GCNNNNNNNGC 8 cut(s) 455, 728, 776, 800, 809, 983, 986, 992
NdeII GATC 3 cut(s) 627, 948, 956
NlaIII CATG 8 cut(s) 221, 402, 430, 499, 607, 722, 820, 889
NlaIV GGNNCC 1 cut(s) 780
NmuCI GTSAC 2 cut(s) 422, 572
NspV TTCGAA 1 cut(s) 1009
PciSI GCTCTTC 1 cut(s) 88
PcsI WCGNNNNNNNCGW 1 cut(s) 1015
PfeI GAWTC 3 cut(s) 263, 745, 897
PflMI CCANNNNNTGG 1 cut(s) 996
PkrI GCNGC 5 cut(s) 64, 733, 891, 979, 988
Psp6I CCWGG 4 cut(s) 389, 726, 781, 995
PspFI CCCAGC 1 cut(s) 892
PspGI CCWGG 4 cut(s) 389, 726, 781, 995
PspN4I GGNNCC 1 cut(s) 780
PspPI GGNCC 2 cut(s) 650, 778
PstNI CAGNNNCTG 2 cut(s) 881, 959
PsuI RGATCY 2 cut(s) 948, 956
RsaI GTAC 2 cut(s) 240, 500
RsaNI GTAC 2 cut(s) 239, 499
SapI GCTCTTC 1 cut(s) 88
SaqAI TTAA 4 cut(s) 354, 521, 807, 923
SatI GCNGC 5 cut(s) 63, 732, 890, 978, 987
Sau3AI GATC 3 cut(s) 627, 948, 956
Sau96I GGNCC 2 cut(s) 650, 778
ScaI AGTACT 1 cut(s) 240
ScrFI CCNGG 4 cut(s) 391, 728, 783, 997
SduI GDGCHC 1 cut(s) 717
SfuI TTCGAA 1 cut(s) 1009
SmlI CTYRAG 1 cut(s) 922
SmoI CTYRAG 1 cut(s) 922
Sse9I AATT 5 cut(s) 23, 213, 505, 526, 906
SsiI CCGC 7 cut(s) 60, 63, 267, 420, 798, 972, 977
SspI AATATT 1 cut(s) 634
SspMI CTAG 2 cut(s) 677, 1030
StyD4I CCNGG 4 cut(s) 389, 726, 781, 995
TaaI ACNGT 2 cut(s) 615, 944
TaiI ACGT 2 cut(s) 544, 579
TaqI TCGA 4 cut(s) 290, 740, 748, 1009
TasI AATT 5 cut(s) 23, 213, 505, 526, 906
TatI WGTACW 2 cut(s) 238, 498
TauI GCSGC 2 cut(s) 65, 980
TfiI GAWTC 3 cut(s) 263, 745, 897
Tru1I TTAA 4 cut(s) 354, 521, 807, 923
Tru9I TTAA 4 cut(s) 354, 521, 807, 923
TseFI GTSAC 2 cut(s) 422, 572
TseI GCWGC 3 cut(s) 731, 889, 986
Tsp45I GTSAC 2 cut(s) 422, 572
TspDTI ATGAA 2 cut(s) 206, 484
Van91I CCANNNNNTGG 1 cut(s) 996
Vha464I CTTAAG 1 cut(s) 922
VneI GTGCAC 1 cut(s) 713
XapI RAATTY 2 cut(s) 213, 505
XspI CTAG 2 cut(s) 677, 1030
ZraI GACGTC 1 cut(s) 542
ZrmI AGTACT 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.