Rh7AG058300

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001329
Physical Location & Seq
Reverse (-)
4001950 .. 4019017
17068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG058300.1

Sequence Viewer

Length: 1059 bp
ATGGGATCTCCCTCAGTCTCTCACTTTGTCAACTTGACACTTGTTGATCTTCCTGGGCTTACAAAAGTTTTTGTTGAGGGTCAGCTCGATAGTATTATGCAGGACATTGAAAATATGGTTCGCTCCTACATTGAGAAGCCCAACAGTATAATTTCAGCAATTTCACCTGCTAATCAAGATCTTGCTACATCTGATGCAATTAAAATCCCTTGTGAAGTGGATCCAATAGGGCAGAAAAGAGAATATCAGTATCAAACCAAAATTGGGGTTCTTTCTAATTGGGAAGGATATTCCAAGGATAATGGAGGGAAACATTCATGTCTCAGGATCTGGACACTTGGGCGGCACTGCAGTAGCAGCCATAGCCTGGGTACGACTTCGGATGGGGCTTCGGGTATTCTAAATGGTTCTGCAGATTACTTGGCAACATTTTGTAGATGTTCTGGGAGATCATGGCATGGGAGGGTATCCAGTAATGACGTGCTTTTAGGATTGATTATAGCTAATTTTGCTGTTTTTCTTTTATGGAAGATAGCAGATCCTGTATTCATGGTCAAGAATTTTACTCTCTCACTATACATTATCACAAGTGGACGTCTTCACACATTGATAACTTCTGCATTTAGTCACTTGGTTGTTGAGGATATTCTTCTCAACATGTTCCTACTCTCTGTCTTTGGGAAAGATGTTGGAAGAACTTTTGGCCCTGAATTTTTGCTAAAGTTGTATCTAGCTGGAGCTATTGGTGGCTCAGTCTTTTACTTGGTGCAATATGTCCTGCTCGCATCAAAGGACGGAGACCTTTCAAACAGTCCCATATTGACATTGGGCGCACAAGGTGCTGTTACTGCTGTGATGTACTTTGACTTGTTACAACCCTGCACATGCATTCCAGCTTTCCTCTACTATCTGAACATATTACTGATGGCCTATCAAATTGGGGATTCCATTTCAAGGATCATACAGGGAAACAGTAGCTCAGGAAATGCACATGTGGGCGGCGCCGCAGTAGCAACTATTGCCTGGGTACGACTTCGTATGGGGCTTCGGGTATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

352

Amino Acids

38.24

Weight (kDa)

7.06

Isoelectric Point (pI)

32.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dynamin_N PF00350 9 - 92 8.4e-13 Dynamin family
Rhomboid PF01694 198 - 342 2.6e-14 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 175
AatII GACGTC 1 cut(s) 598
Acc36I ACCTGC 1 cut(s) 175
AccB1I GGYRCC 1 cut(s) 1001
AccB7I CCANNNNNTGG 1 cut(s) 367
AciI CCGC 3 cut(s) 343, 999, 1005
AclWI GGATC 6 cut(s) 13, 215, 228, 335, 533, 965
AcsI RAATTY 2 cut(s) 559, 710
AcyI GRCGYC 2 cut(s) 595, 1002
AdeI CACNNNGTG 1 cut(s) 839
AfaI GTAC 3 cut(s) 373, 860, 1029
AfiI CCNNNNNNNGG 3 cut(s) 264, 367, 954
AflIII ACRYGT 2 cut(s) 657, 991
AgsI TTSAA 3 cut(s) 110, 807, 954
AjiI CACGTC 1 cut(s) 481
AjnI CCWGG 3 cut(s) 52, 366, 1022
AluBI AGCT 6 cut(s) 85, 503, 734, 740, 896, 978
AluI AGCT 6 cut(s) 85, 503, 734, 740, 896, 978
Alw26I GTCTC 3 cut(s) 22, 326, 792
AlwI GGATC 6 cut(s) 13, 215, 228, 335, 533, 965
AlwNI CAGNNNCTG 2 cut(s) 330, 542
AoxI GGCC 2 cut(s) 703, 927
ApeKI GCWGC 1 cut(s) 357
ApoI RAATTY 2 cut(s) 559, 710
AspLEI GCGC 2 cut(s) 833, 1004
AspS9I GGNCC 1 cut(s) 704
AsuHPI GGTGA 1 cut(s) 156
BamHI GGATCC 1 cut(s) 220
BanI GGYRCC 1 cut(s) 1001
BbsI GAAGAC 1 cut(s) 590
BbvI GCAGC 1 cut(s) 369
BccI CCATC 2 cut(s) 377, 919
BciT130I CCWGG 3 cut(s) 54, 368, 1024
BciVI GTATCC 1 cut(s) 478
BcoDI GTCTC 3 cut(s) 22, 326, 792
BfaI CTAG 1 cut(s) 731
BfmI CTRYAG 2 cut(s) 349, 411
BfoI RGCGCY 1 cut(s) 1005
BfuAI ACCTGC 1 cut(s) 175
BfuI GTATCC 1 cut(s) 478
BglII AGATCT 1 cut(s) 178
BisI GCNGC 4 cut(s) 344, 358, 1000, 1005
BlsI GCNGC 4 cut(s) 345, 359, 1001, 1006
Bme1390I CCNGG 3 cut(s) 54, 368, 1024
BmgBI CACGTC 1 cut(s) 481
BmgT120I GGNCC 1 cut(s) 704
BmiI GGNNCC 2 cut(s) 222, 1003
BmrFI CCNGG 3 cut(s) 54, 368, 1024
BmsI GCATC 2 cut(s) 184, 794
BpiI GAAGAC 1 cut(s) 590
BpmI CTGGAG 1 cut(s) 756
Bpu10I CCTNAGC 1 cut(s) 979
BsaHI GRCGYC 2 cut(s) 595, 1002
BsaI GGTCTC 1 cut(s) 792
BsaJI CCNNGG 4 cut(s) 53, 294, 367, 1023
Bsc4I CCNNNNNNNGG 3 cut(s) 264, 367, 954
Bse1I ACTGG 1 cut(s) 471
BseBI CCWGG 3 cut(s) 54, 368, 1024
BseDI CCNNGG 4 cut(s) 53, 294, 367, 1023
BseGI GGATG 1 cut(s) 388
BseLI CCNNNNNNNGG 3 cut(s) 264, 367, 954
BseMII CTCAG 4 cut(s) 27, 337, 765, 993
BseNI ACTGG 1 cut(s) 471
BseXI GCAGC 1 cut(s) 369
BsgI GTGCAG 1 cut(s) 865
BshFI GGCC 2 cut(s) 705, 929
BshNI GGYRCC 1 cut(s) 1001
BslFI GGGAC 1 cut(s) 798
BslI CCNNNNNNNGG 3 cut(s) 264, 367, 954
BsmAI GTCTC 3 cut(s) 22, 326, 792
BsmFI GGGAC 1 cut(s) 798
BsmI GAATGC 1 cut(s) 888
BsnI GGCC 2 cut(s) 705, 929
Bso31I GGTCTC 1 cut(s) 792
Bsp143I GATC 8 cut(s) 5, 46, 178, 220, 327, 449, 538, 957
BspACI CCGC 3 cut(s) 343, 999, 1005
BspANI GGCC 2 cut(s) 705, 929
BspCNI CTCAG 4 cut(s) 26, 336, 764, 992
BspLI GGNNCC 2 cut(s) 222, 1003
BspMAI CTGCAG 2 cut(s) 353, 415
BspMI ACCTGC 1 cut(s) 175
BspPI GGATC 6 cut(s) 13, 215, 228, 335, 533, 965
BspT107I GGYRCC 1 cut(s) 1001
BspTNI GGTCTC 1 cut(s) 792
BsrI ACTGG 1 cut(s) 471
BssECI CCNNGG 4 cut(s) 53, 294, 367, 1023
BssMI GATC 8 cut(s) 5, 46, 178, 220, 327, 449, 538, 957
BssNI GRCGYC 2 cut(s) 595, 1002
BssT1I CCWWGG 1 cut(s) 294
Bst2UI CCWGG 3 cut(s) 54, 368, 1024
Bst4CI ACNGT 3 cut(s) 146, 812, 974
BstACI GRCGYC 2 cut(s) 595, 1002
BstAPI GCANNNNNTGC 2 cut(s) 839, 1019
BstC8I GCNNGC 1 cut(s) 783
BstDEI CTNAG 4 cut(s) 13, 323, 751, 979
BstF5I GGATG 1 cut(s) 388
BstH2I RGCGCY 1 cut(s) 1005
BstHHI GCGC 2 cut(s) 833, 1004
BstKTI GATC 8 cut(s) 8, 49, 181, 223, 330, 452, 541, 960
BstMAI GTCTC 3 cut(s) 22, 326, 792
BstMBI GATC 8 cut(s) 5, 46, 178, 220, 327, 449, 538, 957
BstMWI GCNNNNNNNGC 7 cut(s) 357, 363, 509, 839, 848, 1010, 1019
BstNI CCWGG 3 cut(s) 54, 368, 1024
BstNSI RCATGY 3 cut(s) 661, 888, 995
BstSCI CCNGG 3 cut(s) 52, 366, 1022
BstSFI CTRYAG 2 cut(s) 349, 411
BstV1I GCAGC 1 cut(s) 369
BstV2I GAAGAC 1 cut(s) 590
BstX2I RGATCY 5 cut(s) 5, 178, 220, 327, 538
BstYI RGATCY 5 cut(s) 5, 178, 220, 327, 538
BsuI GTATCC 1 cut(s) 478
BsuRI GGCC 2 cut(s) 705, 929
BtrI CACGTC 1 cut(s) 481
BtsCI GGATG 1 cut(s) 388
BtsI GCAGTG 1 cut(s) 346
BtsIMutI CAGTG 1 cut(s) 346
BveI ACCTGC 1 cut(s) 175
Cac8I GCNNGC 1 cut(s) 783
CaiI CAGNNNCTG 2 cut(s) 330, 542
CfoI GCGC 2 cut(s) 833, 1004
Cfr13I GGNCC 1 cut(s) 704
Csp6I GTAC 3 cut(s) 372, 859, 1028
CviAII CATG 7 cut(s) 318, 453, 458, 550, 658, 885, 992
CviQI GTAC 3 cut(s) 372, 859, 1028
DdeI CTNAG 4 cut(s) 13, 323, 751, 979
DinI GGCGCC 1 cut(s) 1003
DpnI GATC 8 cut(s) 7, 48, 180, 222, 329, 451, 540, 959
DpnII GATC 8 cut(s) 5, 46, 178, 220, 327, 449, 538, 957
DraIII CACNNNGTG 1 cut(s) 839
Eco130I CCWWGG 1 cut(s) 294
Eco31I GGTCTC 1 cut(s) 792
EcoRII CCWGG 3 cut(s) 52, 366, 1022
EcoT14I CCWWGG 1 cut(s) 294
EcoT22I ATGCAT 1 cut(s) 890
EgeI GGCGCC 1 cut(s) 1003
EheI GGCGCC 1 cut(s) 1003
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 7 cut(s) 321, 456, 461, 553, 661, 888, 995
FaqI GGGAC 1 cut(s) 798
FatI CATG 7 cut(s) 317, 452, 457, 549, 657, 884, 991
Fnu4HI GCNGC 4 cut(s) 344, 358, 1000, 1005
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 4 cut(s) 344, 358, 1000, 1005
FspBI CTAG 1 cut(s) 731
GlaI GCGC 2 cut(s) 832, 1003
GluI GCNGC 4 cut(s) 344, 358, 1000, 1005
GsuI CTGGAG 1 cut(s) 756
HaeII RGCGCY 1 cut(s) 1005
HaeIII GGCC 2 cut(s) 705, 929
HhaI GCGC 2 cut(s) 833, 1004
Hin1I GRCGYC 2 cut(s) 595, 1002
Hin1II CATG 7 cut(s) 321, 456, 461, 553, 661, 888, 995
Hin6I GCGC 2 cut(s) 831, 1002
HinP1I GCGC 2 cut(s) 831, 1002
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 1 cut(s) 944
HphI GGTGA 1 cut(s) 156
Hpy166II GTNNAC 2 cut(s) 31, 593
Hpy188I TCNGA 3 cut(s) 193, 382, 912
Hpy188III TCNNGA 5 cut(s) 176, 325, 331, 556, 981
Hpy8I GTNNAC 2 cut(s) 31, 593
HpyAV CCTTC 1 cut(s) 278
HpyCH4III ACNGT 3 cut(s) 146, 812, 974
HpyCH4IV ACGT 2 cut(s) 480, 595
HpyCH4V TGCA 9 cut(s) 100, 197, 351, 413, 620, 769, 882, 888, 989
HpyF10VI GCNNNNNNNGC 7 cut(s) 357, 363, 509, 839, 848, 1010, 1019
HpyF3I CTNAG 4 cut(s) 13, 323, 751, 979
HpySE526I ACGT 2 cut(s) 480, 595
Hsp92I GRCGYC 2 cut(s) 595, 1002
Hsp92II CATG 7 cut(s) 321, 456, 461, 553, 661, 888, 995
HspAI GCGC 2 cut(s) 831, 1002
KasI GGCGCC 1 cut(s) 1001
Kzo9I GATC 8 cut(s) 5, 46, 178, 220, 327, 449, 538, 957
LmnI GCTCC 2 cut(s) 128, 737
Lsp1109I GCAGC 1 cut(s) 369
LweI GCATC 2 cut(s) 184, 794
MaeI CTAG 1 cut(s) 731
MaeII ACGT 2 cut(s) 480, 595
MaeIII GTNAC 3 cut(s) 626, 844, 870
MalI GATC 8 cut(s) 7, 48, 180, 222, 329, 451, 540, 959
MboI GATC 8 cut(s) 5, 46, 178, 220, 327, 449, 538, 957
MboII GAAGA 5 cut(s) 41, 541, 590, 641, 705
MflI RGATCY 5 cut(s) 5, 178, 220, 327, 538
MluCI AATT 9 cut(s) 150, 159, 198, 261, 277, 505, 559, 710, 936
Mly113I GGCGCC 1 cut(s) 1002
MmeI TCCRAC 1 cut(s) 670
MnlI CCTC 6 cut(s) 22, 70, 299, 456, 634, 911
Mph1103I ATGCAT 1 cut(s) 890
MseI TTAA 1 cut(s) 201
MspR9I CCNGG 3 cut(s) 54, 368, 1024
Mva1269I GAATGC 1 cut(s) 888
MvaI CCWGG 3 cut(s) 54, 368, 1024
MwoI GCNNNNNNNGC 7 cut(s) 357, 363, 509, 839, 848, 1010, 1019
NarI GGCGCC 1 cut(s) 1002
NdeII GATC 8 cut(s) 5, 46, 178, 220, 327, 449, 538, 957
NlaIII CATG 7 cut(s) 321, 456, 461, 553, 661, 888, 995
NlaIV GGNNCC 2 cut(s) 222, 1003
NmuCI GTSAC 1 cut(s) 626
NsiI ATGCAT 1 cut(s) 890
NspI RCATGY 3 cut(s) 661, 888, 995
PaqCI CACCTGC 1 cut(s) 175
PciI ACATGT 2 cut(s) 657, 991
PctI GAATGC 1 cut(s) 888
PfeI GAWTC 1 cut(s) 944
PflMI CCANNNNNTGG 1 cut(s) 367
PkrI GCNGC 4 cut(s) 345, 359, 1001, 1006
PluTI GGCGCC 1 cut(s) 1005
PscI ACATGT 2 cut(s) 657, 991
Psp6I CCWGG 3 cut(s) 52, 366, 1022
PspGI CCWGG 3 cut(s) 52, 366, 1022
PspN4I GGNNCC 2 cut(s) 222, 1003
PspPI GGNCC 1 cut(s) 704
PstI CTGCAG 2 cut(s) 353, 415
PstNI CAGNNNCTG 2 cut(s) 330, 542
PsuI RGATCY 5 cut(s) 5, 178, 220, 327, 538
RsaI GTAC 3 cut(s) 373, 860, 1029
RsaNI GTAC 3 cut(s) 372, 859, 1028
SaqAI TTAA 1 cut(s) 201
SatI GCNGC 4 cut(s) 344, 358, 1000, 1005
Sau3AI GATC 8 cut(s) 5, 46, 178, 220, 327, 449, 538, 957
Sau96I GGNCC 1 cut(s) 704
ScrFI CCNGG 3 cut(s) 54, 368, 1024
SfaNI GCATC 2 cut(s) 184, 794
SfcI CTRYAG 2 cut(s) 349, 411
SfoI GGCGCC 1 cut(s) 1003
Sse9I AATT 9 cut(s) 150, 159, 198, 261, 277, 505, 559, 710, 936
SsiI CCGC 3 cut(s) 343, 999, 1005
SspDI GGCGCC 1 cut(s) 1001
SspMI CTAG 1 cut(s) 731
StyD4I CCNGG 3 cut(s) 52, 366, 1022
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 3 cut(s) 146, 812, 974
TaiI ACGT 2 cut(s) 483, 598
TaqI TCGA 1 cut(s) 87
TasI AATT 9 cut(s) 150, 159, 198, 261, 277, 505, 559, 710, 936
TatI WGTACW 1 cut(s) 858
TauI GCSGC 3 cut(s) 346, 1002, 1007
TfiI GAWTC 1 cut(s) 944
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TscAI CASTG 1 cut(s) 353
TseFI GTSAC 1 cut(s) 626
TseI GCWGC 1 cut(s) 357
Tsp45I GTSAC 1 cut(s) 626
TspDTI ATGAA 2 cut(s) 306, 538
TspGWI ACGGA 1 cut(s) 810
TspRI CASTG 1 cut(s) 353
Van91I CCANNNNNTGG 1 cut(s) 367
XapI RAATTY 2 cut(s) 559, 710
XceI RCATGY 3 cut(s) 661, 888, 995
XcmI CCANNNNNNNNNTGG 1 cut(s) 823
XspI CTAG 1 cut(s) 731
ZraI GACGTC 1 cut(s) 596
Zsp2I ATGCAT 1 cut(s) 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.