Rorug07G0030500

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001329
Physical Location & Seq
Reverse (-)
2110056 .. 2111473
1418 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0030500.1

Sequence Viewer

Length: 732 bp
ATGGCTAACCGCACCACCCACCGGCGCATTTTAGCAACACCAGAACCAGAACCACCAAGGCAGCAGACCAAACCCTCCACTCCTTTAGATCCCACCATGCCCTCTAAACCCTCTTCTAGGCTAAACCCAAATCACAGAATCCGTACAACTCAAAGACCCAGTAGTCAAAACCCACTCCCAAGTCTTGTACACACCAACAATGTTGAGAAACAGAGTAAAGAGAAGCTGCCCAAGAAGCCCAATAGAGAAGTGGATAAGAAGAAAGTTGAGGAGGTGGTGGTGAAGATGGAATTGATGGGTGTCAAAGAAGAAGAGCTTGAAGAAAAAGAGTATGAAAGTAGGAGACTTTCTACTTCTTCTTCTTCAAATTCTTTGGGAGGAAGAAGGAAGTCCTTTTGTGGGTCAGGACAGGTTCAATTGAATAGGTTGGCTGATGTGTTTGCAAGCAATGGTGTGAAAGTGGTTTCAGTTGATATGCCTCCATTTATGCAGATCCATGCTGTGGAATGTGCAAGAAAGACTTGTGATAGCTTAGAAAAATTCACCTCCAAGACTCTAGCTTTGACTCTCAAAAAGGAATTTGATGGGGTATATGGACCAGCATGGCACTGCATTGTAGGAACAAGTTTTGGGTCTTTTGTGACACACTCAGTAAGTGGCTTTATGTATTTCTCAATGGATCAAAAGTTGTACATCCTCTTGTTTAAGACCACAGTACAAAGAGCAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

243

Amino Acids

27.4

Weight (kDa)

9.62

Isoelectric Point (pI)

49.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dynein_light PF01221 152 - 237 2e-27 Dynein light chain type 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 162
AccB7I CCANNNNNTGG 1 cut(s) 502
AciI CCGC 1 cut(s) 10
AclWI GGATC 3 cut(s) 83, 487, 687
AcsI RAATTY 3 cut(s) 367, 539, 578
AfaI GTAC 4 cut(s) 145, 189, 692, 717
AfiI CCNNNNNNNGG 4 cut(s) 21, 117, 399, 502
AgsI TTSAA 4 cut(s) 320, 366, 416, 421
AluBI AGCT 4 cut(s) 226, 316, 531, 560
AluI AGCT 4 cut(s) 226, 316, 531, 560
Alw26I GTCTC 1 cut(s) 337
AlwI GGATC 3 cut(s) 83, 487, 687
ApeKI GCWGC 2 cut(s) 61, 226
ApoI RAATTY 3 cut(s) 367, 539, 578
ArsI GACNNNNNNTTYG 2 cut(s) 544, 576
AspLEI GCGC 1 cut(s) 27
AspS9I GGNCC 1 cut(s) 596
AsuHPI GGTGA 2 cut(s) 292, 535
AvaII GGWCC 1 cut(s) 596
BbvI GCAGC 2 cut(s) 73, 213
BccI CCATC 3 cut(s) 280, 289, 578
BcoDI GTCTC 1 cut(s) 337
BfaI CTAG 2 cut(s) 117, 557
BisI GCNGC 2 cut(s) 62, 227
BlsI GCNGC 2 cut(s) 63, 228
Bme18I GGWCC 1 cut(s) 596
BmgT120I GGNCC 1 cut(s) 596
BmrI ACTGGG 1 cut(s) 153
BmuI ACTGGG 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 56
Bsc4I CCNNNNNNNGG 4 cut(s) 21, 117, 399, 502
Bse118I RCCGGY 1 cut(s) 21
Bse1I ACTGG 1 cut(s) 159
Bse3DI GCAATG 1 cut(s) 454
BseDI CCNNGG 1 cut(s) 56
BseGI GGATG 1 cut(s) 693
BseLI CCNNNNNNNGG 4 cut(s) 21, 117, 399, 502
BseMI GCAATG 1 cut(s) 454
BseMII CTCAG 1 cut(s) 663
BseNI ACTGG 1 cut(s) 159
BseRI GAGGAG 1 cut(s) 284
BseXI GCAGC 2 cut(s) 73, 213
BsiSI CCGG 1 cut(s) 22
BslI CCNNNNNNNGG 4 cut(s) 21, 117, 399, 502
BsmAI GTCTC 1 cut(s) 337
Bsp1407I TGTACA 2 cut(s) 187, 690
Bsp143I GATC 3 cut(s) 88, 492, 679
BspACI CCGC 1 cut(s) 10
BspCNI CTCAG 1 cut(s) 662
BspPI GGATC 3 cut(s) 83, 487, 687
BspQI GCTCTTC 1 cut(s) 306
BsrDI GCAATG 1 cut(s) 454
BsrFI RCCGGY 1 cut(s) 21
BsrGI TGTACA 2 cut(s) 187, 690
BsrI ACTGG 1 cut(s) 159
BssAI RCCGGY 1 cut(s) 21
BssECI CCNNGG 1 cut(s) 56
BssMI GATC 3 cut(s) 88, 492, 679
BssT1I CCWWGG 1 cut(s) 56
Bst4CI ACNGT 1 cut(s) 715
Bst6I CTCTTC 2 cut(s) 118, 306
BstAUI TGTACA 2 cut(s) 187, 690
BstC8I GCNNGC 1 cut(s) 445
BstDEI CTNAG 2 cut(s) 532, 649
BstENI CCTNNNNNAGG 1 cut(s) 115
BstF5I GGATG 1 cut(s) 693
BstHHI GCGC 1 cut(s) 27
BstKTI GATC 3 cut(s) 91, 495, 682
BstMAI GTCTC 1 cut(s) 337
BstMBI GATC 3 cut(s) 88, 492, 679
BstMWI GCNNNNNNNGC 1 cut(s) 235
BstV1I GCAGC 2 cut(s) 73, 213
BstX2I RGATCY 2 cut(s) 88, 492
BstYI RGATCY 2 cut(s) 88, 492
BtsCI GGATG 1 cut(s) 693
BtsI GCAGTG 1 cut(s) 607
BtsIMutI CAGTG 1 cut(s) 607
Cac8I GCNNGC 1 cut(s) 445
CfoI GCGC 1 cut(s) 27
Cfr10I RCCGGY 1 cut(s) 21
Cfr13I GGNCC 1 cut(s) 596
Csp6I GTAC 4 cut(s) 144, 188, 691, 716
CviAII CATG 3 cut(s) 97, 497, 603
CviJI RGCY 9 cut(s) 5, 121, 226, 238, 316, 431, 531, 560, 660
CviKI_1 RGCY 9 cut(s) 5, 121, 226, 238, 316, 431, 531, 560, 660
CviQI GTAC 4 cut(s) 144, 188, 691, 716
DdeI CTNAG 2 cut(s) 532, 649
DpnI GATC 3 cut(s) 90, 494, 681
DpnII GATC 3 cut(s) 88, 492, 679
DrdI GACNNNNNNGTC 1 cut(s) 162
DseDI GACNNNNNNGTC 1 cut(s) 162
Eam1104I CTCTTC 2 cut(s) 118, 306
EarI CTCTTC 2 cut(s) 118, 306
Eco130I CCWWGG 1 cut(s) 56
Eco47I GGWCC 1 cut(s) 596
EcoNI CCTNNNNNAGG 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 56
ErhI CCWWGG 1 cut(s) 56
FaeI CATG 3 cut(s) 100, 500, 606
FaiI YATR 9 cut(s) 98, 333, 476, 488, 498, 592, 594, 604, 665
FalI AAGNNNNNCTT 4 cut(s) 300, 332, 505, 537
FatI CATG 3 cut(s) 96, 496, 602
Fnu4HI GCNGC 2 cut(s) 62, 227
FokI GGATG 1 cut(s) 680
Fsp4HI GCNGC 2 cut(s) 62, 227
FspBI CTAG 2 cut(s) 117, 557
GlaI GCGC 1 cut(s) 26
GluI GCNGC 2 cut(s) 62, 227
HapII CCGG 1 cut(s) 22
HhaI GCGC 1 cut(s) 27
Hin1II CATG 3 cut(s) 100, 500, 606
Hin6I GCGC 1 cut(s) 25
HinP1I GCGC 1 cut(s) 25
HinfI GANTC 3 cut(s) 138, 553, 565
HpaII CCGG 1 cut(s) 22
HphI GGTGA 2 cut(s) 292, 535
Hpy166II GTNNAC 1 cut(s) 190
Hpy188III TCNNGA 1 cut(s) 405
Hpy8I GTNNAC 1 cut(s) 190
HpyAV CCTTC 1 cut(s) 378
HpyCH4III ACNGT 1 cut(s) 715
HpyCH4V TGCA 4 cut(s) 443, 490, 512, 612
HpyF10VI GCNNNNNNNGC 1 cut(s) 235
HpyF3I CTNAG 2 cut(s) 532, 649
Hsp92II CATG 3 cut(s) 100, 500, 606
HspAI GCGC 1 cut(s) 25
Kzo9I GATC 3 cut(s) 88, 492, 679
LguI GCTCTTC 1 cut(s) 306
LpnPI CCDG 7 cut(s) 35, 54, 60, 172, 390, 395, 612
Lsp1109I GCAGC 2 cut(s) 73, 213
MaeI CTAG 2 cut(s) 117, 557
MaeIII GTNAC 1 cut(s) 640
MalI GATC 3 cut(s) 90, 494, 681
MboI GATC 3 cut(s) 88, 492, 679
MfeI CAATTG 1 cut(s) 416
MflI RGATCY 2 cut(s) 88, 492
MluCI AATT 5 cut(s) 290, 367, 416, 539, 578
MlyI GAGTC 2 cut(s) 547, 559
MnlI CCTC 9 cut(s) 85, 112, 121, 262, 265, 371, 489, 556, 707
MseI TTAA 1 cut(s) 705
MspI CCGG 1 cut(s) 22
MunI CAATTG 1 cut(s) 416
MwoI GCNNNNNNNGC 1 cut(s) 235
NdeII GATC 3 cut(s) 88, 492, 679
NlaIII CATG 3 cut(s) 100, 500, 606
NmuCI GTSAC 1 cut(s) 640
PciSI GCTCTTC 1 cut(s) 306
PfeI GAWTC 1 cut(s) 138
PflMI CCANNNNNTGG 1 cut(s) 502
PkrI GCNGC 2 cut(s) 63, 228
PleI GAGTC 2 cut(s) 547, 559
PpsI GAGTC 2 cut(s) 547, 559
PspPI GGNCC 1 cut(s) 596
PsuI RGATCY 2 cut(s) 88, 492
RsaI GTAC 4 cut(s) 145, 189, 692, 717
RsaNI GTAC 4 cut(s) 144, 188, 691, 716
SapI GCTCTTC 1 cut(s) 306
SaqAI TTAA 1 cut(s) 705
SatI GCNGC 2 cut(s) 62, 227
Sau3AI GATC 3 cut(s) 88, 492, 679
Sau96I GGNCC 1 cut(s) 596
SchI GAGTC 2 cut(s) 547, 559
SetI ASST 8 cut(s) 228, 276, 318, 414, 428, 533, 548, 562
SgrAI CRCCGGYG 1 cut(s) 21
SinI GGWCC 1 cut(s) 596
Sse9I AATT 5 cut(s) 290, 367, 416, 539, 578
SsiI CCGC 1 cut(s) 10
SspMI CTAG 2 cut(s) 117, 557
StyI CCWWGG 1 cut(s) 56
TaaI ACNGT 1 cut(s) 715
TasI AATT 5 cut(s) 290, 367, 416, 539, 578
TatI WGTACW 3 cut(s) 187, 690, 715
TfiI GAWTC 1 cut(s) 138
Tru1I TTAA 1 cut(s) 705
Tru9I TTAA 1 cut(s) 705
TscAI CASTG 1 cut(s) 614
TseFI GTSAC 1 cut(s) 640
TseI GCWGC 2 cut(s) 61, 226
Tsp45I GTSAC 1 cut(s) 640
TspDTI ATGAA 1 cut(s) 348
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 1 cut(s) 614
Van91I CCANNNNNTGG 1 cut(s) 502
VpaK11BI GGWCC 1 cut(s) 596
XagI CCTNNNNNAGG 1 cut(s) 115
XapI RAATTY 3 cut(s) 367, 539, 578
XcmI CCANNNNNNNNNTGG 1 cut(s) 247
XspI CTAG 2 cut(s) 117, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.