Rhomboid family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 79 |
| AfaI | GTAC | 2 cut(s) | 74, 104 |
| AluBI | AGCT | 1 cut(s) | 23 |
| AluI | AGCT | 1 cut(s) | 23 |
| ApoI | RAATTY | 1 cut(s) | 79 |
| Bsp1407I | TGTACA | 1 cut(s) | 72 |
| BsrGI | TGTACA | 1 cut(s) | 72 |
| BstAUI | TGTACA | 1 cut(s) | 72 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 29 |
| Csp6I | GTAC | 2 cut(s) | 73, 103 |
| CviAII | CATG | 1 cut(s) | 70 |
| CviJI | RGCY | 2 cut(s) | 23, 113 |
| CviKI_1 | RGCY | 2 cut(s) | 23, 113 |
| CviQI | GTAC | 2 cut(s) | 73, 103 |
| FaeI | CATG | 1 cut(s) | 73 |
| FaiI | YATR | 5 cut(s) | 46, 65, 71, 99, 120 |
| FatI | CATG | 1 cut(s) | 69 |
| FspEI | CC | 8 cut(s) | 31, 34, 54, 74, 75, 95, 119, 120 |
| Hin1II | CATG | 1 cut(s) | 73 |
| Hpy166II | GTNNAC | 1 cut(s) | 103 |
| Hpy8I | GTNNAC | 1 cut(s) | 103 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 29 |
| Hsp92II | CATG | 1 cut(s) | 73 |
| MluCI | AATT | 1 cut(s) | 79 |
| MnlI | CCTC | 1 cut(s) | 42 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 29 |
| NlaIII | CATG | 1 cut(s) | 73 |
| RsaI | GTAC | 2 cut(s) | 74, 104 |
| RsaNI | GTAC | 2 cut(s) | 73, 103 |
| SetI | ASST | 1 cut(s) | 25 |
| SgeI | CNNG | 3 cut(s) | 82, 88, 104 |
| Sse9I | AATT | 1 cut(s) | 79 |
| TasI | AATT | 1 cut(s) | 79 |
| TatI | WGTACW | 1 cut(s) | 72 |
| TspDTI | ATGAA | 2 cut(s) | 58, 107 |
| XapI | RAATTY | 1 cut(s) | 79 |