Prupe.5G207300_v2.0.a1

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001329
Physical Location & Seq
Reverse (-)
16538594 .. 16542688
4095 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G207300.1

Sequence Viewer

Length: 1035 bp
ATGCAGAGGCTCCTTCGTTTGAAACTAGCCTCAAACCTCCCCAAAAAGCTCTCAAATTCCACCACCACCACCACCTCTTCAATCTTCCACTTTCAACCCCACAAAACGCTTTCTCTCACCAAGCCACCCCAAACCCGAAACTACTTTTTTAGCTCCTTTACCACACCCCCCCTTCACCATCATCTTCATTCATGGAGGTCGCAGCACACTCTCACACAAAAAATTAATGGGTTTCTCTTCAACCCATTACTCAAACGGCAATTTTGTATGGGTTTCTCAAATACCCTTTTGAGAGTCTCAAGCAAGAGTCTTTCAGATTTCAGACTTGGCTTTCTCAGAGCCCAATTTCCCAAACAGAGTTTTCGGTTCAACCCAAATTTTACTTCTCCTCGGAGTCCCTGGAGATCATGGTTCCGAAGGGTATCTGATGGTGATGTGGTTTTGGGATTGATTATAGCTAATGTTGCTGTTTTTATTTTATGGAGGATTGCAGATCCTAGCTTCATGGCGAAGAATTTTACTATTTCATTAGACAATGTTACAAGTGGACGTCTGCACACATTGATAACTTCTGCATTCAGTCATATGGATGTTGGCCATATCATTTCTAACATGATTGGACTATATTTTTTTGGGACGAATATTGGAAGAGTTTTTGGGCCTGAATTTTTGCTGAAGTTGTATCTAGCTGGGGCTGTTGGTGGCTCGGTCTGTTTCTTGGTGCACAAAGCCTACATGGCCGCGTCATCGAAGGGCCGGCAACCATGGAACATGGACCCATCAAAAATACCGGGACTGGGTGCAAGCGGGGCTGTTAATGCTATCATGTTGCTTGATATATTCCTCTTCCCAAAATCTACCCTCTACTTGGAGTTTATCATACCAGTTCCCGCCATCCTGCTGGGGATCTTTCTAATTGGGAAAGATGTCTTGGGGATAATAGAGGGAGACAGTCATATCTCAGGAGCTGCACACTTGGGGGGTGCTGCAGTTGCAGCCATAGCCTGGGCACGACTTCGGAAGGGGCGTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

345

Amino Acids

38.3

Weight (kDa)

10.9

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 553
AccB7I CCANNNNNTGG 1 cut(s) 1005
AccII CGCG 1 cut(s) 743
AciI CCGC 3 cut(s) 741, 807, 891
AclWI GGATC 2 cut(s) 488, 914
AcoI YGGCCR 2 cut(s) 595, 738
AcsI RAATTY 4 cut(s) 55, 376, 514, 665
AcuI CTGAAG 1 cut(s) 695
AcyI GRCGYC 1 cut(s) 550
AfiI CCNNNNNNNGG 3 cut(s) 797, 868, 1005
AgsI TTSAA 5 cut(s) 22, 81, 95, 241, 370
AjnI CCWGG 2 cut(s) 398, 1004
AjuI GAANNNNNNNTTGG 4 cut(s) 367, 399, 914, 946
AluBI AGCT 6 cut(s) 49, 153, 458, 501, 689, 968
AluI AGCT 6 cut(s) 49, 153, 458, 501, 689, 968
Alw21I GWGCWC 1 cut(s) 726
Alw26I GTCTC 2 cut(s) 301, 942
Alw44I GTGCAC 1 cut(s) 722
AlwI GGATC 2 cut(s) 488, 914
AlwNI CAGNNNCTG 1 cut(s) 968
AoxI GGCC 4 cut(s) 595, 659, 738, 754
ApaLI GTGCAC 1 cut(s) 722
ApeKI GCWGC 4 cut(s) 202, 968, 986, 995
ApoI RAATTY 4 cut(s) 55, 376, 514, 665
AseI ATTAAT 1 cut(s) 225
AspS9I GGNCC 3 cut(s) 659, 754, 775
AsuC2I CCSGG 1 cut(s) 792
AsuHPI GGTGA 3 cut(s) 109, 167, 443
AvaII GGWCC 1 cut(s) 775
BaeGI GKGCMC 2 cut(s) 726, 1012
BalI TGGCCA 1 cut(s) 597
BanII GRGCYC 1 cut(s) 343
Bbv12I GWGCWC 1 cut(s) 726
BbvI GCAGC 4 cut(s) 214, 955, 973, 1007
BccI CCATC 4 cut(s) 186, 422, 787, 902
BceAI ACGGC 1 cut(s) 272
BciT130I CCWGG 2 cut(s) 400, 1006
BcnI CCSGG 1 cut(s) 792
BcoDI GTCTC 2 cut(s) 301, 942
BfaI CTAG 3 cut(s) 26, 498, 686
BfmI CTRYAG 1 cut(s) 987
BglI GCCNNNNNGGC 1 cut(s) 737
BisI GCNGC 5 cut(s) 203, 741, 969, 987, 996
BlsI GCNGC 5 cut(s) 204, 742, 970, 988, 997
Bme1390I CCNGG 3 cut(s) 400, 792, 1006
Bme18I GGWCC 1 cut(s) 775
BmgT120I GGNCC 3 cut(s) 659, 754, 775
BmiI GGNNCC 3 cut(s) 11, 413, 777
BmrFI CCNGG 3 cut(s) 400, 792, 1006
BmrI ACTGGG 1 cut(s) 806
BmuI ACTGGG 1 cut(s) 806
BpmI CTGGAG 1 cut(s) 421
BpuEI CTTGAG 1 cut(s) 283
BpuMI CCSGG 1 cut(s) 792
BsaHI GRCGYC 1 cut(s) 550
BsaJI CCNNGG 4 cut(s) 389, 398, 764, 1005
Bsc4I CCNNNNNNNGG 3 cut(s) 797, 868, 1005
Bse118I RCCGGY 1 cut(s) 756
Bse1I ACTGG 2 cut(s) 801, 884
BseBI CCWGG 2 cut(s) 400, 1006
BseDI CCNNGG 4 cut(s) 389, 398, 764, 1005
BseGI GGATG 2 cut(s) 595, 894
BseLI CCNNNNNNNGG 3 cut(s) 797, 868, 1005
BseMII CTCAG 2 cut(s) 349, 975
BseNI ACTGG 2 cut(s) 801, 884
BseRI GAGGAG 1 cut(s) 378
BseSI GKGCMC 2 cut(s) 726, 1012
BseXI GCAGC 4 cut(s) 214, 955, 973, 1007
BseYI CCCAGC 2 cut(s) 689, 901
BsgI GTGCAG 2 cut(s) 539, 954
Bsh1236I CGCG 1 cut(s) 743
BshFI GGCC 4 cut(s) 597, 661, 740, 756
BsiHKAI GWGCWC 1 cut(s) 726
BsiSI CCGG 2 cut(s) 757, 791
BslFI GGGAC 3 cut(s) 381, 649, 807
BslI CCNNNNNNNGG 3 cut(s) 797, 868, 1005
BsmAI GTCTC 2 cut(s) 301, 942
BsmFI GGGAC 3 cut(s) 381, 649, 807
BsmI GAATGC 1 cut(s) 575
BsnI GGCC 4 cut(s) 597, 661, 740, 756
Bsp1286I GDGCHC 3 cut(s) 343, 726, 1012
Bsp143I GATC 3 cut(s) 404, 493, 906
Bsp19I CCATGG 1 cut(s) 764
BspACI CCGC 3 cut(s) 741, 807, 891
BspANI GGCC 4 cut(s) 597, 661, 740, 756
BspCNI CTCAG 2 cut(s) 348, 974
BspFNI CGCG 1 cut(s) 743
BspLI GGNNCC 3 cut(s) 11, 413, 777
BspMAI CTGCAG 1 cut(s) 991
BspPI GGATC 2 cut(s) 488, 914
BsrFI RCCGGY 1 cut(s) 756
BsrI ACTGG 2 cut(s) 801, 884
BssAI RCCGGY 1 cut(s) 756
BssECI CCNNGG 4 cut(s) 389, 398, 764, 1005
BssMI GATC 3 cut(s) 404, 493, 906
BssNI GRCGYC 1 cut(s) 550
BssT1I CCWWGG 1 cut(s) 764
Bst2UI CCWGG 2 cut(s) 400, 1006
Bst4CI ACNGT 1 cut(s) 953
Bst6I CTCTTC 4 cut(s) 82, 242, 643, 851
BstACI GRCGYC 1 cut(s) 550
BstC8I GCNNGC 2 cut(s) 758, 805
BstDEI CTNAG 2 cut(s) 335, 961
BstDSI CCRYGG 1 cut(s) 764
BstF5I GGATG 2 cut(s) 595, 894
BstFNI CGCG 1 cut(s) 743
BstKTI GATC 3 cut(s) 407, 496, 909
BstMAI GTCTC 2 cut(s) 301, 942
BstMBI GATC 3 cut(s) 404, 493, 906
BstMWI GCNNNNNNNGC 7 cut(s) 464, 737, 809, 818, 992, 995, 1001
BstNI CCWGG 2 cut(s) 400, 1006
BstSCI CCNGG 3 cut(s) 398, 790, 1004
BstSFI CTRYAG 1 cut(s) 987
BstSLI GKGCMC 2 cut(s) 726, 1012
BstUI CGCG 1 cut(s) 743
BstV1I GCAGC 4 cut(s) 214, 955, 973, 1007
BstX2I RGATCY 2 cut(s) 493, 906
BstXI CCANNNNNNTGG 1 cut(s) 901
BstYI RGATCY 2 cut(s) 493, 906
BsuRI GGCC 4 cut(s) 597, 661, 740, 756
BtgI CCRYGG 1 cut(s) 764
BtsCI GGATG 2 cut(s) 595, 894
Cac8I GCNNGC 2 cut(s) 758, 805
CaiI CAGNNNCTG 1 cut(s) 968
Cfr10I RCCGGY 1 cut(s) 756
Cfr13I GGNCC 3 cut(s) 659, 754, 775
CseI GACGC 1 cut(s) 732
CviAII CATG 8 cut(s) 192, 408, 505, 613, 736, 765, 772, 826
DdeI CTNAG 2 cut(s) 335, 961
DpnI GATC 3 cut(s) 406, 495, 908
DpnII GATC 3 cut(s) 404, 493, 906
EaeI YGGCCR 2 cut(s) 595, 738
Eam1104I CTCTTC 4 cut(s) 82, 242, 643, 851
EarI CTCTTC 4 cut(s) 82, 242, 643, 851
Eco130I CCWWGG 1 cut(s) 764
Eco24I GRGCYC 1 cut(s) 343
Eco47I GGWCC 1 cut(s) 775
Eco57I CTGAAG 1 cut(s) 695
EcoRII CCWGG 2 cut(s) 398, 1004
EcoT14I CCWWGG 1 cut(s) 764
EcoT38I GRGCYC 1 cut(s) 343
ErhI CCWWGG 1 cut(s) 764
FaeI CATG 8 cut(s) 195, 411, 508, 616, 739, 768, 775, 829
FaqI GGGAC 3 cut(s) 381, 649, 807
FatI CATG 8 cut(s) 191, 407, 504, 612, 735, 764, 771, 825
FauI CCCGC 2 cut(s) 800, 898
FauNDI CATATG 1 cut(s) 585
Fnu4HI GCNGC 5 cut(s) 203, 741, 969, 987, 996
FokI GGATG 2 cut(s) 602, 881
FriOI GRGCYC 1 cut(s) 343
Fsp4HI GCNGC 5 cut(s) 203, 741, 969, 987, 996
FspBI CTAG 3 cut(s) 26, 498, 686
GluI GCNGC 5 cut(s) 203, 741, 969, 987, 996
GsaI CCCAGC 2 cut(s) 693, 905
GsuI CTGGAG 1 cut(s) 421
HaeIII GGCC 4 cut(s) 597, 661, 740, 756
HapII CCGG 2 cut(s) 757, 791
HgaI GACGC 1 cut(s) 732
Hin1I GRCGYC 1 cut(s) 550
Hin1II CATG 8 cut(s) 195, 411, 508, 616, 739, 768, 775, 829
HinfI GANTC 3 cut(s) 294, 307, 394
HpaII CCGG 2 cut(s) 757, 791
HphI GGTGA 3 cut(s) 109, 167, 443
Hpy166II GTNNAC 2 cut(s) 548, 724
Hpy188I TCNGA 8 cut(s) 316, 323, 338, 393, 416, 427, 1020, 1034
Hpy188III TCNNGA 1 cut(s) 963
Hpy8I GTNNAC 2 cut(s) 548, 724
HpyAV CCTTC 5 cut(s) 23, 182, 411, 745, 1015
HpyCH4III ACNGT 1 cut(s) 953
HpyCH4IV ACGT 1 cut(s) 550
HpyCH4V TGCA 9 cut(s) 4, 491, 556, 575, 724, 803, 971, 989, 995
HpyF10VI GCNNNNNNNGC 7 cut(s) 464, 737, 809, 818, 992, 995, 1001
HpyF3I CTNAG 2 cut(s) 335, 961
HpySE526I ACGT 1 cut(s) 550
Hsp92I GRCGYC 1 cut(s) 550
Hsp92II CATG 8 cut(s) 195, 411, 508, 616, 739, 768, 775, 829
KroI GCCGGC 1 cut(s) 756
KroNI GCCGGC 1 cut(s) 758
Kzo9I GATC 3 cut(s) 404, 493, 906
LmnI GCTCC 3 cut(s) 15, 158, 965
Lsp1109I GCAGC 4 cut(s) 214, 955, 973, 1007
MaeI CTAG 3 cut(s) 26, 498, 686
MaeII ACGT 1 cut(s) 550
MaeIII GTNAC 1 cut(s) 538
MalI GATC 3 cut(s) 406, 495, 908
MboI GATC 3 cut(s) 404, 493, 906
MboII GAAGA 7 cut(s) 69, 76, 176, 229, 523, 660, 838
MflI RGATCY 2 cut(s) 493, 906
MhlI GDGCHC 3 cut(s) 343, 726, 1012
MlsI TGGCCA 1 cut(s) 597
MluCI AATT 8 cut(s) 55, 222, 260, 344, 376, 514, 665, 915
MluNI TGGCCA 1 cut(s) 597
MlyI GAGTC 3 cut(s) 303, 316, 403
MnlI CCTC 9 cut(s) 40, 47, 85, 189, 399, 477, 854, 872, 937
Mox20I TGGCCA 1 cut(s) 597
MroNI GCCGGC 1 cut(s) 756
MscI TGGCCA 1 cut(s) 597
MseI TTAA 2 cut(s) 225, 816
MslI CAYNNNNRTG 1 cut(s) 588
Msp20I TGGCCA 1 cut(s) 597
MspI CCGG 2 cut(s) 757, 791
MspR9I CCNGG 3 cut(s) 400, 792, 1006
Mva1269I GAATGC 1 cut(s) 575
MvaI CCWGG 2 cut(s) 400, 1006
MvnI CGCG 1 cut(s) 743
MwoI GCNNNNNNNGC 7 cut(s) 464, 737, 809, 818, 992, 995, 1001
NaeI GCCGGC 1 cut(s) 758
NciI CCSGG 1 cut(s) 792
NcoI CCATGG 1 cut(s) 764
NdeI CATATG 1 cut(s) 585
NdeII GATC 3 cut(s) 404, 493, 906
NgoMIV GCCGGC 1 cut(s) 756
NlaIII CATG 8 cut(s) 195, 411, 508, 616, 739, 768, 775, 829
NlaIV GGNNCC 3 cut(s) 11, 413, 777
PcsI WCGNNNNNNNCGW 1 cut(s) 1024
PctI GAATGC 1 cut(s) 575
PdiI GCCGGC 1 cut(s) 758
PflMI CCANNNNNTGG 1 cut(s) 1005
PkrI GCNGC 5 cut(s) 204, 742, 970, 988, 997
PleI GAGTC 3 cut(s) 302, 315, 402
PpsI GAGTC 3 cut(s) 302, 315, 402
PshBI ATTAAT 1 cut(s) 225
Psp6I CCWGG 2 cut(s) 398, 1004
PspFI CCCAGC 2 cut(s) 689, 901
PspGI CCWGG 2 cut(s) 398, 1004
PspN4I GGNNCC 3 cut(s) 11, 413, 777
PspPI GGNCC 3 cut(s) 659, 754, 775
PstI CTGCAG 1 cut(s) 991
PstNI CAGNNNCTG 1 cut(s) 968
PsuI RGATCY 2 cut(s) 493, 906
RseI CAYNNNNRTG 1 cut(s) 588
SaqAI TTAA 2 cut(s) 225, 816
SatI GCNGC 5 cut(s) 203, 741, 969, 987, 996
Sau3AI GATC 3 cut(s) 404, 493, 906
Sau96I GGNCC 3 cut(s) 659, 754, 775
SchI GAGTC 3 cut(s) 303, 316, 403
ScrFI CCNGG 3 cut(s) 400, 792, 1006
SduI GDGCHC 3 cut(s) 343, 726, 1012
SfcI CTRYAG 1 cut(s) 987
SinI GGWCC 1 cut(s) 775
SmiMI CAYNNNNRTG 1 cut(s) 588
SmlI CTYRAG 1 cut(s) 298
SmoI CTYRAG 1 cut(s) 298
Sse9I AATT 8 cut(s) 55, 222, 260, 344, 376, 514, 665, 915
SsiI CCGC 3 cut(s) 741, 807, 891
SspI AATATT 1 cut(s) 643
SspMI CTAG 3 cut(s) 26, 498, 686
StyD4I CCNGG 3 cut(s) 398, 790, 1004
StyI CCWWGG 1 cut(s) 764
TaaI ACNGT 1 cut(s) 953
TaiI ACGT 1 cut(s) 553
TaqI TCGA 1 cut(s) 749
TaqII GACCGA 1 cut(s) 697
TasI AATT 8 cut(s) 55, 222, 260, 344, 376, 514, 665, 915
TauI GCSGC 1 cut(s) 743
Tru1I TTAA 2 cut(s) 225, 816
Tru9I TTAA 2 cut(s) 225, 816
TseI GCWGC 4 cut(s) 202, 968, 986, 995
TspDTI ATGAA 4 cut(s) 176, 180, 493, 516
Van91I CCANNNNNTGG 1 cut(s) 1005
VneI GTGCAC 1 cut(s) 722
VpaK11BI GGWCC 1 cut(s) 775
VspI ATTAAT 1 cut(s) 225
XapI RAATTY 4 cut(s) 55, 376, 514, 665
XspI CTAG 3 cut(s) 26, 498, 686
ZraI GACGTC 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.